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3 results on '"Fangzhi Tan"'

1. Organized cannabinoid receptor distribution in neurons revealed by super-resolution fluorescence imaging

Author

Hui Li, Jie Yang, Cuiping Tian, Min Diao, Quan Wang, Simeng Zhao, Shanshan Li, Fangzhi Tan, Tian Hua, Ya Qin, Chao-Po Lin, Dylan Deska-Gauthier, Garth J. Thompson, Ying Zhang, Wenqing Shui, Zhi-Jie Liu, Tong Wang, and Guisheng Zhong

Subjects
Science
Abstract

Despite the importance of G-protein-coupled receptors in many cellular functions, their intracellular organisation is largely unknown. The authors identified periodically repeating clusters of cannabinoid receptor 1 hotspots within neuronal axons that are dynamically regulated by CB1 agonists.

Published
2020
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2. AAV-ie enables safe and efficient gene transfer to inner ear cells

Author

Fangzhi Tan, Cenfeng Chu, Jieyu Qi, Wenyan Li, Dan You, Ke Li, Xin Chen, Weidong Zhao, Cheng Cheng, Xiaoyi Liu, Yunbo Qiao, Bing Su, Shuijin He, Chao Zhong, Huawei Li, Renjie Chai, and Guisheng Zhong

Subjects
Science
Abstract

There are currently few AAV vectors that can effectively target the diverse cell types of the inner ear. Here the authors design AAV-ie for gene delivery to the mouse cochlea, targeting cochlear supporting cells.

Published
2019
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3. Organized cannabinoid receptor distribution in neurons revealed by super-resolution fluorescence imaging

Author

Jie Yang, Shanshan Li, Simeng Zhao, Tong Wang, Min Diao, Cuiping Tian, Zhi-Jie Liu, Fangzhi Tan, Tian Hua, Garth John Thompson, Ying Zhang, Chao-Po Lin, Dylan Deska-Gauthier, Wenqing Shui, Guisheng Zhong, Ya Qin, Hui Li, and Quan Wang

Subjects
Male, 0301 basic medicine, Agonist, Fluorescence-lifetime imaging microscopy, Cannabinoid receptor, medicine.drug_class, medicine.medical_treatment, Science, General Physics and Astronomy, Article, General Biochemistry, Genetics and Molecular Biology, Rats, Sprague-Dawley, 03 medical and health sciences, 0302 clinical medicine, Receptor, Cannabinoid, CB1, medicine, Animals, Super-resolution microscopy, Receptor, lcsh:Science, Cells, Cultured, Cytoskeleton, G protein-coupled receptor, Mice, Knockout, Neurons, Multidisciplinary, Chemistry, musculoskeletal, neural, and ocular physiology, Brain, Fluorescence recovery after photobleaching, food and beverages, General Chemistry, Cellular neuroscience, Axons, Molecular Imaging, Cell biology, Mice, Inbred C57BL, 030104 developmental biology, Microscopy, Fluorescence, nervous system, Female, lcsh:Q, lipids (amino acids, peptides, and proteins), Cannabinoid, psychological phenomena and processes, 030217 neurology & neurosurgery, Intracellular, Fluorescence Recovery After Photobleaching
Abstract

G-protein-coupled receptors (GPCRs) play important roles in cellular functions. However, their intracellular organization is largely unknown. Through investigation of the cannabinoid receptor 1 (CB1), we discovered periodically repeating clusters of CB1 hotspots within the axons of neurons. We observed these CB1 hotspots interact with the membrane-associated periodic skeleton (MPS) forming a complex crucial in the regulation of CB1 signaling. Furthermore, we found that CB1 hotspot periodicity increased upon CB1 agonist application, and these activated CB1 displayed less dynamic movement compared to non-activated CB1. Our results suggest that CB1 forms periodic hotspots organized by the MPS as a mechanism to increase signaling efficacy upon activation., Despite the importance of G-protein-coupled receptors in many cellular functions, their intracellular organisation is largely unknown. The authors identified periodically repeating clusters of cannabinoid receptor 1 hotspots within neuronal axons that are dynamically regulated by CB1 agonists.

Published
2020

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