52 results on '"Reddy, E. P."'
Search Results
2. Styryl sulfonyl compounds inhibit translation of cyclin D1 in mantle cell lymphoma cells.
- Author
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Prasad A, Park IW, Allen H, Zhang X, Reddy MV, Boominathan R, Reddy EP, and Groopman JE
- Subjects
- Apoptosis drug effects, Cell Line, Tumor, Eukaryotic Initiation Factor-4E physiology, Extracellular Signal-Regulated MAP Kinases physiology, Forkhead Box Protein O1, Forkhead Transcription Factors metabolism, Glycine pharmacology, Humans, Lymphoma, Mantle-Cell genetics, Lymphoma, Mantle-Cell pathology, Mitogen-Activated Protein Kinase Kinases physiology, Phosphoinositide-3 Kinase Inhibitors, Phosphorylation, Protein Kinases physiology, Proto-Oncogene Proteins c-akt physiology, Proto-Oncogene Proteins c-myc antagonists & inhibitors, Signal Transduction drug effects, TOR Serine-Threonine Kinases, p38 Mitogen-Activated Protein Kinases physiology, raf Kinases physiology, Antineoplastic Agents pharmacology, Cyclin D1 genetics, Glycine analogs & derivatives, Lymphoma, Mantle-Cell drug therapy, Protein Biosynthesis drug effects, Sulfones pharmacology
- Abstract
Mantle cell lymphoma (MCL) is characterized by the uncontrolled overexpression of cyclin D1. Styryl sulfonyl compounds have shown potent antitumor activity against MCL by inducing cell-cycle arrest and apoptosis. However, the exact molecular mechanism by which these compounds function is yet to be elucidated. Here, we show that the prototypical styryl sulfonyl compound ON 01910.Na decreased cyclin D1 and c-Myc protein levels in MCL cells, whereas mRNA levels of cyclin D1 were minimally affected. Notably, ON 01910.Na suppressed eukaryotic translation initiation factor 4E (eIF4E)-mediated cyclin D1 mRNA translation, decreased levels of phosphorylated Akt, mammalian target of Rapamycin (mTOR) and eIF4E-binding protein (eIF4E-BP), lowered the cap site binding activity of eIF4E and directly inhibited activity of phosphatidylinositol-3 kinase (PI-3K). Analysis of apoptotic signaling pathways revealed that ON 01910.Na induced the release of cytochrome c from mitochondria, altered expression of Bcl-2 family of proteins and stimulated activation of caspases. Taken together, styryl sulfonyls can cause a rapid decrease of cyclin D1 by blocking cyclin D1 mRNA translation through inhibition of the PI-3K/Akt/mTOR/eIF4E-BP signaling pathway and triggering a cytochrome c-dependent apoptotic pathway in MCL cells.
- Published
- 2009
- Full Text
- View/download PDF
3. Evaluation of the novel mitotic modulator ON 01910.Na in pancreatic cancer and preclinical development of an ex vivo predictive assay.
- Author
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Jimeno A, Chan A, Cusatis G, Zhang X, Wheelhouse J, Solomon A, Chan F, Zhao M, Cosenza SC, Ramana Reddy MV, Rudek MA, Kulesza P, Donehower RC, Reddy EP, and Hidalgo M
- Subjects
- Animals, Antimetabolites, Antineoplastic therapeutic use, Biopsy, Fine-Needle, Cell Line, Tumor, Cyclin B metabolism, Cyclin B1, Deoxycytidine analogs & derivatives, Deoxycytidine pharmacology, Deoxycytidine therapeutic use, Female, Glycine pharmacology, Glycine therapeutic use, Humans, Mice, Mice, Nude, Pancreatic Neoplasms metabolism, Pancreatic Neoplasms pathology, Predictive Value of Tests, Sulfones therapeutic use, cdc25 Phosphatases metabolism, Gemcitabine, Antimetabolites, Antineoplastic pharmacology, Drug Resistance, Neoplasm drug effects, Glycine analogs & derivatives, Pancreatic Neoplasms drug therapy, Sulfones pharmacology, Xenograft Model Antitumor Assays
- Abstract
The pupose of this study was to evaluate the activity of ON 01910.Na, a mitotic inhibitor, in in vitro and in vivo models of pancreatic cancer and to discover biomarkers predictive of efficacy. Successive in vitro and in vivo models were used; these included cell line-derived and patient-derived tumors from our PancXenoBank, a live collection of freshly generated pancreatic cancer xenografts. ON 01910.Na showed equivalent activity to gemcitabine against pancreatic cancer cell lines in vitro. The activity of the agent correlated with suppression of phospho-CDC25C and cyclin B1. These markers were optimized for a fine-needle aspirate ex vivo rapid assay. Cyclin B1 mRNA evaluation yielded the most optimal combination of accuracy and reproducibility. Next, nine patient-derived tumors from the PancXenoBank were profiled using the assay developed in cell lines and treated with ON01910.Na for 28 days. Two cases were cataloged as potential responders and seven as resistants. There was a correlation between the ex vivo assay and sensitivity to the tested agent, as the two cases prospectively identified as sensitive met prespecified criteria for response. Of the seven tumors of predictive resistant, only one was found to be sensitive to ON 01910.Na. In addition, there was a good correlation between cyclin B1 downregulation ex vivo and changes in cyclin B1 protein post-treatment. The novel mitotic inhibitor, ON 01910.Na, showed activity in preclinical model of pancreatic cancer. A rapid assay was rationally developed that not only identified cases sensitive to ON 01910.Na, but also anticipated the pharmacodynamic events occurring after in vivo exposure.
- Published
- 2009
- Full Text
- View/download PDF
4. JNK signaling in apoptosis.
- Author
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Dhanasekaran DN and Reddy EP
- Subjects
- Cell Nucleus enzymology, Humans, Mitochondria enzymology, Phosphorylation, Apoptosis genetics, MAP Kinase Kinase 4 metabolism, Signal Transduction
- Abstract
Jun N-terminal kinases or JNKs play a critical role in death receptor-initiated extrinsic as well as mitochondrial intrinsic apoptotic pathways. JNKs activate apoptotic signaling by the upregulation of pro-apoptotic genes through the transactivation of specific transcription factors or by directly modulating the activities of mitochondrial pro- and antiapoptotic proteins through distinct phosphorylation events. This review analyses our present understanding of the role of JNK in apoptotic signaling and the various mechanisms by which JNK promotes apoptosis.
- Published
- 2008
- Full Text
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5. Celecoxib and a novel COX-2 inhibitor ON09310 upregulate death receptor 5 expression via GADD153/CHOP.
- Author
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He Q, Luo X, Jin W, Huang Y, Reddy MV, Reddy EP, and Sheikh MS
- Subjects
- Apoptosis drug effects, Celecoxib, Cell Line, Tumor, Cyclooxygenase 2 Inhibitors therapeutic use, Drug Screening Assays, Antitumor, Humans, Indoles therapeutic use, Pyrazoles therapeutic use, Sulfonamides therapeutic use, TNF-Related Apoptosis-Inducing Ligand metabolism, Up-Regulation drug effects, Cyclooxygenase 2 Inhibitors pharmacology, Gene Expression Regulation, Neoplastic drug effects, Indoles pharmacology, Neoplasm Proteins metabolism, Neoplasms prevention & control, Pyrazoles pharmacology, Receptors, TNF-Related Apoptosis-Inducing Ligand biosynthesis, Sulfonamides pharmacology, Transcription Factor CHOP metabolism
- Abstract
Cyclooxygenase-2 (COX-2) inhibitors are promising anticancer agents but their long-term use at high doses is associated with adverse cardiovascular events. The molecular mechanisms underlying the anticancer or toxic cardiovascular effects of COX-2 inhibitors remain unknown. Here we report that COX-2-selective celecoxib and a novel COX-2 inhibitor ON09310 upregulate death receptor 5 (DR5) and cooperate with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), the ligand for DR5, to induce apoptosis in COX-2-positive and -negative cancer cells. We also show that both agents engage GADD153/CHOP to transcriptionally upregulate DR5 expression; GADD153/CHOP is a C/EBP homologous transcription factor implicated in cellular stress response and apoptosis. Based on our results, we propose that (1) these agents appear to mediate their effects, at least in part, by engaging GADD153/CHOP to activate DR5-dependent apoptotic pathway and (2) their regulation of GADD153/CHOP and DR5 expression appears to occur independent of their COX-2 inhibitory effects. Our results also indicate that ON09310 is generally more potent than celecoxib and, at lower concentration, strongly cooperates with TRAIL to induce apoptosis. Taken together, our findings form the basis for future in-depth studies to further explore the utility of TRAIL and/or agonistic anti-DR5 antibodies in combination with low-dose COX-2 inhibitors as a rational approach for cancer prevention and treatment.
- Published
- 2008
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6. Disruption of transforming growth factor-beta signaling through beta-spectrin ELF leads to hepatocellular cancer through cyclin D1 activation.
- Author
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Kitisin K, Ganesan N, Tang Y, Jogunoori W, Volpe EA, Kim SS, Katuri V, Kallakury B, Pishvaian M, Albanese C, Mendelson J, Zasloff M, Rashid A, Fishbein T, Evans SR, Sidawy A, Reddy EP, Mishra B, Johnson LB, Shetty K, and Mishra L
- Subjects
- Animals, Carrier Proteins genetics, Cell Line, Tumor, Cyclin D, Cyclins antagonists & inhibitors, Humans, Liver Neoplasms, Experimental metabolism, Mice, Mice, Knockout, Microfilament Proteins deficiency, Microfilament Proteins genetics, Phosphorylation, Receptors, Transforming Growth Factor beta metabolism, Retinoblastoma metabolism, Signal Transduction genetics, Spectrin deficiency, Spectrin genetics, Transforming Growth Factor beta2 metabolism, Transforming Growth Factor beta2 physiology, Tumor Suppressor Proteins genetics, Tumor Suppressor Proteins physiology, Carcinoma, Hepatocellular etiology, Carcinoma, Hepatocellular metabolism, Carrier Proteins physiology, Cyclins metabolism, Liver Neoplasms, Experimental etiology, Microfilament Proteins physiology, Signal Transduction physiology, Spectrin physiology, Transforming Growth Factor beta2 antagonists & inhibitors
- Abstract
Transforming growth factor-beta (TGF-beta) signaling members, TGF-beta receptor type II (TBRII), Smad2, Smad4 and Smad adaptor, embryonic liver fodrin (ELF), are prominent tumor suppressors in gastrointestinal cancers. Here, we show that 40% of elf(+/-) mice spontaneously develop hepatocellular cancer (HCC) with markedly increased cyclin D1, cyclin-dependent kinase 4 (Cdk4), c-Myc and MDM2 expression. Reduced ELF but not TBRII, or Smad4 was observed in 8 of 9 human HCCs (P<0.017). ELF and TBRII are also markedly decreased in human HCC cell lines SNU-398 and SNU-475. Restoration of ELF and TBRII in SNU-398 cells markedly decreases cyclin D1 as well as hyperphosphorylated-retinoblastoma (hyperphosphorylated-pRb). Thus, we show that TGF-beta signaling and Smad adaptor ELF suppress human hepatocarcinogenesis, potentially through cyclin D1 deregulation. Loss of ELF could serve as a primary event in progression toward a fully transformed phenotype and could hold promise for new therapeutic approaches in human HCCs.
- Published
- 2007
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7. Hematopoietic cytokine receptor signaling.
- Author
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Baker SJ, Rane SG, and Reddy EP
- Subjects
- Animals, Cytokines physiology, Hematopoietic Stem Cells enzymology, Hematopoietic Stem Cells metabolism, Humans, Cytokines metabolism, Hematopoiesis physiology, Hematopoietic Stem Cells physiology, Receptors, Cytokine physiology, Signal Transduction physiology
- Abstract
Hematopoiesis is the cumulative result of intricately regulated signaling pathways that are mediated by cytokines and their receptors. Proper culmination of these diverse pathways forms the basis for an orderly generation of different cell types. Recent studies conducted over the past 10-15 years have revealed that hematopoietic cytokine receptor signaling is largely mediated by a family of tyrosine kinases termed Janus kinases (JAKs) and their downstream transcription factors termed STATs (signal transducers and activators of transcription). Aberration in these pathways, such as that caused by the recently identified JAK2V617F mutation, is an underlying cause for diseases such as leukemias and other myeloproliferative disorders. This recent discovery, when coupled with the fact that STATs are activated by oncoproteins such as BCR-ABL, underscores the importance of the JAK-STAT pathway in both normal cellular development and disease states.
- Published
- 2007
- Full Text
- View/download PDF
8. Evaluation of novel cell cycle inhibitors in mantle cell lymphoma.
- Author
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Park IW, Reddy MV, Reddy EP, and Groopman JE
- Subjects
- Antineoplastic Agents chemistry, Apoptosis drug effects, Blotting, Western, Caspase 3 metabolism, Caspase Inhibitors, Cell Cycle Proteins metabolism, Cell Division drug effects, Cell Line, Tumor, Cell Survival drug effects, Cysteine Proteinase Inhibitors pharmacology, Dose-Response Relationship, Drug, Drug Screening Assays, Antitumor, Flow Cytometry, G2 Phase drug effects, Humans, In Situ Nick-End Labeling, Lymphoma, Mantle-Cell metabolism, Lymphoma, Mantle-Cell pathology, Molecular Structure, Styrenes chemistry, Styrenes pharmacology, Sulfones chemistry, Antineoplastic Agents pharmacology, Cell Cycle drug effects, Sulfones pharmacology
- Abstract
Signature abnormalities in the cell cycle and apoptotic pathway have been identified in mantle cell lymphoma (MCL), affording the opportunity to develop targeted therapies. In this study, we tested a novel class of kinase inhibitors, styryl sulfones, which differ from prior cell cycle inhibitors in that they are not related to purines or pyrimidines. We observed that two closely related compounds, ON013100 and ON01370, altered the growth and cell cycle status of MCL lines and potently inhibited the expression of several important molecules, including cyclin-dependent kinase 4, p53, mouse double minute 2 (MDM2), and cyclin D as well as increased cyclin B expression. Using both terminal deoxy transferase uridine triphosphate nick end-labelling and poly ADP-ribose polymerase assays, we found that these compounds caused apoptosis in MCL cells. In addition, using molecular analyses, we observed the modulation of caspase-3 activity but not the expression of B-cell lymphoma family molecules. Next, we investigated the cytotoxicity of the MCL lines upon treatment with styryl sulfone compounds in combination with other currently used chemotherapeutic agents, such as doxorubicin (DOX) or vincristine (VCR). We found that the combination of DOX plus styryl sulfone or VCR plus styryl sulfone increased cytotoxicity by one log scale, compared with the single styryl sulfone compound. Thus, styryl sulfones alone, or in combination with chemotherapeutic agents, present attractive opportunities for new drug development in MCL.
- Published
- 2007
- Full Text
- View/download PDF
9. Scaffold proteins of MAP-kinase modules.
- Author
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Dhanasekaran DN, Kashef K, Lee CM, Xu H, and Reddy EP
- Subjects
- Adaptor Proteins, Signal Transducing chemistry, Adaptor Proteins, Signal Transducing metabolism, Animals, Humans, Mitogen-Activated Protein Kinases chemistry, Mitogen-Activated Protein Kinases metabolism, Nuclear Matrix-Associated Proteins chemistry, Nuclear Matrix-Associated Proteins metabolism, Adaptor Proteins, Signal Transducing physiology, Mitogen-Activated Protein Kinases physiology, Nuclear Matrix-Associated Proteins physiology
- Abstract
Mitogen-activated protein kinases (MAPKs) regulate critical signaling pathways involved in cell proliferation, differentiation and apoptosis. Recent studies have shown that a novel class of scaffold proteins mediates the structural and functional organization of the three-tier MAPK module. By linking the MAP3K, MAP2K and MAPK into a multienzyme complex, these MAPK-specific scaffold proteins provide an insulated physical conduit through which signals from the respective MAPK can be transmitted to the appropriate spatiotemporal cellular loci. Scaffold proteins play a determinant role in modulating the signaling strength of their cognate MAPK module by regulating the signal amplitude and duration. The scaffold proteins themselves are finely regulated resulting in dynamic intra- and inter-molecular interactions that can modulate the signaling outputs of MAPK modules. This review focuses on defining the diverse mechanisms by which these scaffold proteins interact with their respective MAPK modules and the role of such interactions in the spatiotemporal organization as well as context-specific signaling of the different MAPK modules.
- Published
- 2007
- Full Text
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10. Granulocyte colony-stimulating factor-induced upregulation of Jak3 transcription during granulocytic differentiation is mediated by the cooperative action of Sp1 and Stat3.
- Author
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Mangan JK, Tantravahi RV, Rane SG, and Reddy EP
- Subjects
- Animals, Cells, Cultured, Electrophoretic Mobility Shift Assay, Janus Kinase 3, Mice, Protein-Tyrosine Kinases metabolism, Up-Regulation, Cell Differentiation, Gene Expression Regulation, Granulocyte Colony-Stimulating Factor pharmacology, Granulocytes cytology, Protein-Tyrosine Kinases genetics, STAT3 Transcription Factor metabolism, Sp1 Transcription Factor metabolism, Transcription, Genetic
- Abstract
We previously demonstrated that Jak3 is a primary response gene for G-CSF and ectopic overexpression of Jak3 can accelerate granulocytic differentiation of normal mouse bone marrow cells induced by G-CSF and GM-CSF. To gain insight into the regulation of G-CSF-induced transcription of Jak3, we constructed deletion and linker scanning mutants of the Jak3 promoter sequences and performed luciferase reporter assays in the murine myeloid cell line 32Dcl3, with and without G-CSF stimulation. These experiments showed that mutation of a -67 to -85 element, which contained a putative Sp1 binding site, or mutation of a -44 to -53 GAS element resulted in a marked reduction of Jak3 promoter activity. Electrophoretic mobility shift assays revealed that Sp1 and Stat3 present in nuclear lysates of 32Dcl3 cells stimulated with G-CSF can bind to the -67 to -85 element and -44 to -53 GAS element, respectively. In addition, cotransfection of a constitutively active mutant of Stat3 along with a Jak3 promoter/luciferase reporter resulted in enhanced Jak3 promoter activity. Together, these results demonstrate that activation of Jak3 transcription during G-CSF- induced granulocytic differentiation is mediated by the combined action of Sp1 and Stat3, a mechanism also shown to be important in IL-6-induced monocytic differentiation.
- Published
- 2006
- Full Text
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11. Critical interactions between TGF-beta signaling/ELF, and E-cadherin/beta-catenin mediated tumor suppression.
- Author
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Katuri V, Tang Y, Li C, Jogunoori W, Deng CX, Rashid A, Sidawy AN, Evans S, Reddy EP, Mishra B, and Mishra L
- Subjects
- Animals, Cadherins physiology, Carrier Proteins physiology, Cell Adhesion, Epithelial Cells physiology, Gastrointestinal Neoplasms physiopathology, Gene Expression Profiling, Mice, Microfilament Proteins physiology, Signal Transduction, Smad4 Protein biosynthesis, Smad4 Protein genetics, beta Catenin physiology, Carrier Proteins biosynthesis, DNA-Binding Proteins, Gastrointestinal Neoplasms genetics, Microfilament Proteins biosynthesis, Transforming Growth Factor beta physiology
- Abstract
Inactivation of the transforming growth factor-beta (TGF-beta) pathway occurs often in malignancies of the gastrointestinal (GI) system. However, only a fraction of sporadic GI tumors exhibit inactivating mutations in early stages of cancer formation, suggesting that other mechanisms play a critical role in the inactivation of this pathway. Here, we show a wide range of GI tumors, including those of the stomach, liver and colon in elf+/- and elf+/- / Smad4+/- mutant mice. We found that embryonic liver fodrin (ELF), a beta-Spectrin originally identified in endodermal stem/progenitor cells committed to foregut lineage, possesses potent antioncogenic activity and is frequently inactivated in GI cancers. Specifically, E-cadherin accumulation at cell-cell contacts and E-cadherin-beta-catenin-dependent epithelial cell-cell adhesion is disrupted in elf+/- / Smad4+/- mutant gastric epithelial cells, and could be rescued by ectopic expression of full-length elf, but not Smad3 or Smad4. Subcellular fractionation revealed that E-cadherin is expressed mainly at the cell membrane after TGF-beta stimulation. In contrast, elf+/- / Smad4+/- mutant tissues showed abnormal distribution of E-cadherin that could be rescued by overexpression of ELF but not Smad3 or Smad4. Our results identify a group of common lethal malignancies in which inactivation of TGF-beta signaling, which is essential for tumor suppression, is disrupted by inactivation of the ELF adaptor protein.
- Published
- 2006
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12. Characterization of an alternatively spliced AATYK mRNA: expression pattern of AATYK in the brain and neuronal cells.
- Author
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Baker SJ, Sumerson R, Reddy CD, Berrebi AS, Flynn DC, and Reddy EP
- Subjects
- Amino Acid Sequence, Animals, Apoptosis Regulatory Proteins, Base Sequence, Blotting, Western, Brain cytology, Carcinoma genetics, Carcinoma metabolism, Cell Differentiation, Cloning, Molecular, DNA Primers chemistry, DNA, Complementary genetics, Embryonal Carcinoma Stem Cells, Gene Expression, Humans, Immunoenzyme Techniques, Molecular Sequence Data, Neoplastic Stem Cells metabolism, Polymerase Chain Reaction, Protein Biosynthesis, Protein-Tyrosine Kinases metabolism, Rats, Reverse Transcriptase Polymerase Chain Reaction, Transcription, Genetic, Tumor Cells, Cultured, Alternative Splicing genetics, Brain enzymology, Neurons enzymology, Protein-Tyrosine Kinases genetics, RNA, Messenger biosynthesis
- Abstract
The AATYK gene encodes a tyrosine kinase whose expression is up-regulated during the apoptosis and differentiation of 32Dcl3 myeloblastic cells. Because high levels of AATYK mRNA have also been detected in the brain, and because these transcripts differ in size from that observed in the 32Dcl3 cell line, it was of interest to determine whether this gene encodes mRNAs that are alternatively spliced and whether these mRNAs are expressed in a tissue-specific manner. We have isolated a novel, alternatively spliced AATYK mRNA using cDNA library screening and RT-PCR, whose expression is readily detected in the brain but not myeloid cells. Western blot analysis revealed that the AATYK protein was expressed in virtually all regions of the adult rat brain in which neurons are present, including olfactory bulb, forebrain, cortex, midbrain, cerebellum and pons. Immunohistochemical labeling of adult brain sections showed the highest levels of AATYK expression in the cerebellum and olfactory bulb. Expression of AATYK was also up-regulated as a function of RA-induced neuronal differentiation of p19 embryonal carcinoma cells, supporting a role for this protein in mature neurons and neuronal differentiation.
- Published
- 2001
- Full Text
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13. Janus kinases: components of multiple signaling pathways.
- Author
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Rane SG and Reddy EP
- Subjects
- Animals, Cytokines physiology, Enzyme Activation, Humans, Janus Kinase 1, Janus Kinase 2, Protein-Tyrosine Kinases chemistry, Structure-Activity Relationship, Substrate Specificity, Protein-Tyrosine Kinases metabolism, Proto-Oncogene Proteins, Signal Transduction
- Abstract
Cytoplasmic Janus protein tyrosine kinases (JAKs) are crucial components of diverse signal transduction pathways that govern cellular survival, proliferation, differentiation and apoptosis. Evidence to date, indicates that JAK kinase function may integrate components of diverse signaling cascades. While it is likely that activation of STAT proteins may be an important function attributed to the JAK kinases, it is certainly not the only function performed by this key family of cytoplasmic tyrosine kinases. Emerging evidence indicates that phosphorylation of cytokine and growth factor receptors may be the primary functional attribute of JAK kinases. The JAK-triggered receptor phosphorylation can potentially be a rate-limiting event for a successful culmination of downstream signaling events. In support of this hypothesis, it has been found that JAK kinase function is required for optimal activation of the Src-kinase cascade, the Ras-MAP kinase pathway, the PI3K-AKT pathway and STAT signaling following the interaction of cytokine/interferon receptors with their ligands. Aberrations in JAK kinase activity, that may lead to derailment of one or more of the above mentioned pathways could disrupt normal cellular responses and result in disease states. Thus, over-activation of JAK kinases has been implicated in tumorigenesis. In contrast, loss of JAK kinase function has been found to result in disease states such as severe-combined immunodeficiency. In summary, optimal JAK kinase activity is a critical determinant of normal transmission of cytokine and growth factor signals.
- Published
- 2000
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14. IL-3 signaling and the role of Src kinases, JAKs and STATs: a covert liaison unveiled.
- Author
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Reddy EP, Korapati A, Chaturvedi P, and Rane S
- Subjects
- Animals, Apoptosis, Cytokines metabolism, Humans, Janus Kinase 1, Phosphatidylinositol 3-Kinases metabolism, Receptors, Interleukin-3 metabolism, STAT1 Transcription Factor, Transcriptional Activation, DNA-Binding Proteins metabolism, Interleukin-3 metabolism, Protein-Tyrosine Kinases metabolism, Signal Transduction, Trans-Activators metabolism, src-Family Kinases metabolism
- Abstract
Hematopoiesis is the cumulative result of intricately regulated signal transduction cascades that are mediated by cytokines and their cognate receptors. Proper culmination of these diverse signaling pathways forms the basis for an orderly generation of different cell types and aberrations in these pathways is an underlying cause for diseases such as cancer. Over the past several years, downstream events initiated upon cytokine/growth factor stimulation have been a major focus of biomedical research. As a result, several key concepts have emerged allowing a better understanding of the complex signaling processes. A group of novel transcription factors, termed signal transducers and activators of transcription (STATs) appear to orchestrate the downstream events propagated by cytokine/growth factor interactions with their cognate receptors. Until recently, the JAK proteins were considered to be the tyrosine kinases, which dictated the levels of phosphorylation and activation of STAT proteins, forming the basis of the JAK-STAT model. However, over the past few years, increasing evidence has accumulated which indicates that at least some of the STAT protein activation may be mediated by members of the Src gene family following cytokine/growth factor stimulation. Studies have demonstrated that the Src-family of tyrosine kinases can phosphorylate and activate certain STAT proteins, in lieu of JAK kinases. In such a scenario, JAK kinases may be more crucial to phosphorylation of the cytokine/growth factor receptors and in the process create docking sites on the receptors for binding of SH2-containing proteins such as STATs, Src-kinases and other signaling intermediates. Tyrosine phosphorylation and activation of STAT proteins can be achieved either by JAKs or Src-kinases depending on the nature of STAT that is being activated. This forms the basis for the JAK-Src-STAT model proposed in this review. The concerted action of JAK kinases, members of the Src-kinase family and STAT proteins, leads to cell proliferation and cell survival, the end-point of the cytokine/growth factor stimulus. Oncogene (2000).
- Published
- 2000
- Full Text
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15. Oncogenic mutant of Galpha12 stimulates cell proliferation through cycloxygenase-2 signaling pathway.
- Author
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Dermott JM, Reddy MR, Onesime D, Reddy EP, and Dhanasekaran N
- Subjects
- 3T3 Cells, Animals, Cell Division genetics, Cyclooxygenase 2, Fibroblasts physiology, Mice, Mutation, Cell Transformation, Neoplastic genetics, Fibroblasts pathology, GTP-Binding Proteins genetics, Isoenzymes physiology, Prostaglandin-Endoperoxide Synthases physiology, Signal Transduction genetics
- Abstract
Expression of the GTPase-deficient, activated mutant alpha-subunit of the heterotrimeric G protein G12 (Galpha12QL) leads to the neoplastic transformation of fibroblast cell lines. The mitogenic pathway regulated by Galpha12QL includes an extensive signaling network involving several small GTPases and various kinases. In addition, Galpha12QL has been shown to potentiate the serum-induced phospholipase-A2 activity in NIH3T3 cells. In the present study, we demonstrate that cycloxygenase-2 (COX-2) pathway is involved in the mitogenic pathway activated by Galpha12QL. Expression of Galpha12QL and not Galpha13QL, stimulates the serum-induced release of arachidonic acid in NIH3T3 cells. Furthermore, expression of Galpha12QL or the stimulation of wild-type Galpha12 induces the expression of COX-2. Our results also indicate that the COX-2 inhibitor acutely disrupts the DNA-synthesis stimulated by Galpha12QL in NIH3T3 cells. These studies, for the first time, identify the crucial role of COX-2 in Galpha12-mediated regulation of cell proliferation and suggest a role for prostaglandin-derived autocrine loop in Galpha12-mediated signaling pathways.
- Published
- 1999
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16. The myb gene family in cell growth, differentiation and apoptosis.
- Author
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Oh IH and Reddy EP
- Subjects
- Gene Expression, Humans, Apoptosis, Cell Differentiation, Cell Division, Oncogenes genetics
- Abstract
The myb gene family consists of three members, named A, B and c-myb which encode nuclear proteins that function as transcriptional transactivators. Proteins encoded by these three genes exhibit a tripartate structure with an N-terminal DNA-binding domain, a central transactivation domain and a C-terminal regulatory domain. These proteins exhibit highest homology in their DNA binding domains and appear to bind DNA with overlapping sequence specificities. Transactivation by myb gene family varies considerably depending on cell type and promoter context suggesting a dependence on interaction with other cell type specific co-factors. While the C-terminal domains of A-Myb and c-Myb proteins exert a negative regulatory effect on their transcriptional transactivation function, the C-terminal domain of B-Myb appears to function as a positive regulator of this activity. One or more of these proteins interact with other transcription factors such as Ets-2, CEBP and NF-M. In addition, expression of these genes is cell cycle-regulated and inhibition of their expression with antisense oligonucleotides has been found to affect cell cycle-progression, cell division and/or differentiation. Members of the myb gene family exhibit different temporal and spatial expression patterns suggesting a distinctive function for each of these genes. Gene knockout experiments show that these genes play an essential role in development. Loss of c-myb function results in embryonic lethality due to failure of fetal hepatic hematopoiesis. A-myb null mutant mice, on the other hand are viable but exhibit growth abnormalities, and defects in spermatogenesis and female breast development. While the role of c-myb in oncogenesis is well established, future experiments are likely to provide further clues regarding the role of A-myb and B-myb in tumorigenesis.
- Published
- 1999
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17. The v-myc oncogene.
- Author
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Lee CM and Reddy EP
- Subjects
- Animals, Apoptosis, Cell Differentiation, Cell Division, Cell Transformation, Neoplastic genetics, Gene Expression Regulation, Neoplastic, Humans, Macrophage Colony-Stimulating Factor genetics, Genes, myc physiology
- Abstract
v-myc is the viral homolog of c-myc transduced by several acute transforming retroviruses, many of which encode this gene as a Gag-Myc fusion protein. The v-myc oncogene can transform several lineages of mammalian and avian cells either alone or in cooperation with other oncogenes. While the Gag portion of the Gag-Myc fusion protein and the nuclear localization signal each appear to be dispensable for transformation, the N- and C-termini of the Myc sequence have been found to be essential for transformation. All v-myc genes contain point mutations which seem to confer a greater potency to v-myc in the process of transformation, proliferation, and apoptosis. In v-myc-transformed myelomonocytic cells, secondary events occur, such as the expression of colony stimulating factor-1 (CSF-1) which play a critical role in immortalization and subsequent tumor progression. Inhibition of the autocrine loop of CSF-1 was found to induce apoptosis in the immortalized cells. While overexpression of v-Myc blocks terminal differentiation of hematopoietic cells, this is not sufficient to block the differentiation of certain neural and skeletal muscle cells. Recent developments on the effects of v-myc on cell growth, transformation, differentiation and apoptosis are discussed in this review.
- Published
- 1999
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18. Modulation of life and death by the TNF receptor superfamily.
- Author
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Baker SJ and Reddy EP
- Subjects
- Animals, Antigens, CD physiology, CD40 Antigens metabolism, Calcium-Calmodulin-Dependent Protein Kinases physiology, Carrier Proteins physiology, Co-Repressor Proteins, Fas-Associated Death Domain Protein, Humans, JNK Mitogen-Activated Protein Kinases, Molecular Chaperones, Multigene Family, Proteins metabolism, Proteins physiology, Receptor-Interacting Protein Serine-Threonine Kinases, Receptors, Tumor Necrosis Factor, Type I, TNF Receptor-Associated Factor 1, TNF Receptor-Associated Factor 3, Tumor Necrosis Factor-alpha physiology, fas Receptor physiology, Adaptor Proteins, Signal Transducing, Apoptosis genetics, Intracellular Signaling Peptides and Proteins, Mitogen-Activated Protein Kinases, Nuclear Proteins, Receptors, Tumor Necrosis Factor physiology, Signal Transduction
- Abstract
The tumor necrosis factor receptor (TNFR) superfamily represents a growing family, with over 20 members having been identified thus far in mammalian cells. These proteins share significant homologies in their extracellular ligand binding domains and intracellular effector (death) domains. These receptors appear to transmit their signals via protein-protein interactions, which convey either a death or survival signal. Isolation and characterization of death domain containing proteins (TRADD, FADD/MORT-1, RIP), TRAF domain containing proteins (TRAF1-6) as well as new members and adaptor proteins such as DAXX have provided new insights to our understanding of signaling mechanisms associated with this family of receptors. While the death signals seem to be associated with the activation of both the caspase and JUN kinase pathways, the survival signals are mediated via the activation of the NF-kappaB pathway.
- Published
- 1998
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19. Src kinases and not JAKs activate STATs during IL-3 induced myeloid cell proliferation.
- Author
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Chaturvedi P, Reddy MV, and Reddy EP
- Subjects
- Animals, Apoptosis, CSK Tyrosine-Protein Kinase, Cell Division, Cell Line, Cell Line, Transformed, DNA metabolism, Enzyme Activation, Janus Kinase 2, Mice, Mitogen-Activated Protein Kinase 1 metabolism, Mutagenesis, Protein-Tyrosine Kinases genetics, STAT3 Transcription Factor, src-Family Kinases, DNA-Binding Proteins metabolism, Interleukin-3 pharmacology, Mitogens pharmacology, Protein-Tyrosine Kinases metabolism, Proto-Oncogene Proteins, Trans-Activators metabolism
- Abstract
Interaction of IL-3 with its receptor is known to activate STAT-3 via phosphorylation of Tyrosine 701, which facilitates its dimerization and translocation to the nucleus, leading to the transcription of its target genes. In this communication, we have investigated the nature of tyrosine kinases that mediate STAT-3 phosphorylation during IL-3-mediated activation of myeloid cell proliferation. Our results show that interaction of IL-3 with its receptor leads to the activation of c-Src kinase activity, which in turn facilitates the binding of c-Src to STAT-3. This association leads to the phosphorylation of STAT-3, allowing this transcription factor to translocate to the nucleus. Expression of a dominant negative mutant of src (AMSrc) in these cells results in a block to IL-3 mediated phosphorylation of STAT-3, and its ability to bind to DNA. On the other hand, expression of a dominant negative mutant of JAK2 (JAK2KE) had no effect on IL-3-mediated activation of STAT-3. Our results also show that AMSrc does not affect the phosphorylation of JAK2, suggesting that JAK and STAT phosphorylation events are mediated by two independent pathways. Inhibition of c-Src activation by AMSrc, which leads to a block to STAT-3 activation, results in a dramatic inhibition of cell proliferation mediated by IL-3. However, expression of AMSrc does not activate apoptotic pathways. In contrast, expression of JAK2KE results in accelerated apoptosis of 32Dcl3 cells grown in the absence of IL-3 with concomitant down-regulation of Erk-2 kinase activity. These results suggest that Src family kinases mediate the phosphorylation of STATs and play a critical role in signal transduction pathways associated with myeloid cell proliferation while JAK kinases mediate the activation of Erk-2 pathway which appears to provide antiapoptotic signals. Thus the activation of JAKs and STATs appear to be two independent but related events, which dictate two separate biological outcomes, the combination of which results in proliferation and survival of myeloid precursor cells.
- Published
- 1998
- Full Text
- View/download PDF
20. AATYK: a novel tyrosine kinase induced during growth arrest and apoptosis of myeloid cells.
- Author
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Gaozza E, Baker SJ, Vora RK, and Reddy EP
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Cell Differentiation, Cells, Cultured, Cloning, Molecular, Cytokines physiology, Enzyme Induction, Granulocyte Colony-Stimulating Factor physiology, In Vitro Techniques, Mice, Molecular Sequence Data, Protein Biosynthesis, Protein Structure, Tertiary, Protein-Tyrosine Kinases isolation & purification, Transcription, Genetic, Up-Regulation, src Homology Domains, Apoptosis genetics, Bone Marrow Cells enzymology, Protein-Tyrosine Kinases genetics
- Abstract
Apoptosis, or programmed cell death, is a process where developmental or environmental stimuli activate a genetic program to implement a series of events that culminate in cell death. To study the nature of genes that are induced during the apoptotic death of myeloid precursor cells, we utilized the 32Dcl3 cell line, which is derived from normal mouse bone marrow, is non-tumorigenic and diploid. These cells are strictly dependent on IL-3 for growth and apoptose when deprived of IL-3. However, when these cells are transferred to medium containing G-CSF, the cell number increases 4-5-fold and after 12 days the entire population is differentiated into granulocytes followed by apoptotic death. In our search for genes that are induced during apoptosis and/or terminal differentiation of 32Dcl3 cells, we identified a novel gene termed AATYK (Apoptosis Associated Tyrosine Kinase), whose expression is dramatically upregulated during IL-3 deprivation as well as G-CSF-induced terminal differentiation. In this report, we describe the sequence of the cDNA clone, derived from the mRNA transcript of this gene. These studies show that this gene encodes a protein with a tyrosine kinase domain at the N-terminal end and a proline-rich domain at the C-terminal end. We also report that the expression of this gene is blocked in v-abl or bcr-abl transformed myeloid cells which are unable to apoptose when grown in the absence of IL-3. However, AATYK expression is induced in 32D cells transformed by the v-abl gene when these cells are incubated in the presence of DMSO, which induces growth arrest and apoptotic death of the cells. On the other hand, DMSO fails to induce apoptosis or AATYK expression in 32D cells transformed by the bcr-abl oncogene, suggesting that AATYK expression may be a necessary pre-requisite for the induction of growth arrest and/or apoptosis of myeloid precursor cells.
- Published
- 1997
- Full Text
- View/download PDF
21. cAMP-induced NF-kappaB (p50/relB) binding to a c-myb intronic enhancer correlates with c-myb up-regulation and inhibition of erythroleukemia cell differentiation.
- Author
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Suhasini M, Reddy CD, Reddy EP, DiDonato JA, and Pilz RB
- Subjects
- Animals, Colforsin pharmacology, Cyclic AMP analogs & derivatives, Genes, Reporter, Leukemia, Erythroblastic, Acute metabolism, Leukemia, Erythroblastic, Acute pathology, Mice, Protein Binding, RNA, Messenger genetics, RNA, Messenger metabolism, Subcellular Fractions metabolism, Transcription Factors metabolism, Tumor Cells, Cultured, Cell Differentiation genetics, Cyclic AMP pharmacology, Enhancer Elements, Genetic, Introns, Leukemia, Erythroblastic, Acute genetics, NF-kappa B metabolism, Oncogenes
- Abstract
During hexamethylene bisactamide (HMBA)-induced differentiation of murine erythroleukemia (MEL) cells erythroid genes are transcriptionally activated while c-myb and several other nuclear proto-oncogenes are down-regulated. Differentiation is inhibited by cAMP analogues and the adenyl cyclase-stimulating agent forskolin. We found that these drugs prevented the late down-regulation of c-myb which is known to be critical for MEL cell differentiation. Since the increase in c-myb mRNA levels was due to increased mRNA transcription, we examined the transcription factors NF-kappaB and AP-1 which have been implicated in the regulation of c-myb expression. Binding of MEL cell nuclear proteins to a NF-kappaB recognition sequence in c-myb intron I was strongly induced by 8-Br-cAMP or forskolin; induction was delayed and correlated with the up-regulation of c-myb. The cAMP-induced NF-kappaB complex contained p50 and RelB; in co-transfection assays, p50 and RelB transactivated a reporter construct containing the c-myb intronic NF-kappaB site upstream of a minimal promoter. 8-Br-cAMP and forskolin also increased the DNA binding activity of AP-1 complexes containing JunB, JunD and c-Fos in MEL cells which could cooperate with NF-kappaB. We conclude that inhibition of MEL cell differentiation by pharmacological doses of cAMP can be explained by the up-regulation of c-myb which is mediated, at least in part, by NF-kappaB p50/RelB heterodimers.
- Published
- 1997
- Full Text
- View/download PDF
22. Arrest of spermatogenesis and defective breast development in mice lacking A-myb.
- Author
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Toscani A, Mettus RV, Coupland R, Simpkins H, Litvin J, Orth J, Hatton KS, and Reddy EP
- Subjects
- Animals, Female, Gene Expression, Gene Targeting, Germ-Line Mutation, Infertility, Male genetics, Male, Mammary Glands, Animal anatomy & histology, Mammary Glands, Animal embryology, Mammary Glands, Animal growth & development, Meiosis, Mice, Molecular Sequence Data, Pregnancy, Proto-Oncogene Proteins genetics, Proto-Oncogene Proteins c-myb, Spermatogenesis genetics, Stem Cells, Testis anatomy & histology, Testis embryology, Testis growth & development, Trans-Activators genetics, Mammary Glands, Animal physiology, Proto-Oncogene Proteins physiology, Spermatogenesis physiology, Testis physiology, Trans-Activators physiology
- Abstract
The Myb gene family currently consists of three members, named A-, B- and c-myb. These genes encode nuclear proteins that bind DNA in a sequence-specific manner and function as regulators of transcription. In adult male mice, A-myb is expressed predominantly in male germ cells. In female mice, A-myb is expressed in breast ductal epithelium, mainly during pregnancy-induced ductal branching and alveolar development. We report here that mice homozygous for a germline mutation in A-myb develop to term but show defects in growth after birth and male infertility due to a block in spermatogenesis. Morphological examination of the testes of A-myb-/- males revealed that the germ cells enter meiotic prophase and arrest at pachytene. In adult homozygous null A-myb female mice, the breast epithelial compartment showed underdevelopment of breast tissue following pregnancy and the female mice were unable to nurse their newborn pups. These results demonstrate that A-myb plays a critical role in spermatogenesis and mammary gland development.
- Published
- 1997
- Full Text
- View/download PDF
23. Structural organization and chromosomal mapping of JAK3 locus.
- Author
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Kumar A, Toscani A, Rane S, and Reddy EP
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Chromosome Mapping, Cloning, Molecular, Conserved Sequence, Humans, In Situ Hybridization, Fluorescence, Janus Kinase 1, Janus Kinase 2, Janus Kinase 3, Mice, Molecular Sequence Data, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Transcription, Genetic, Chromosomes, Human, Pair 19, Protein-Tyrosine Kinases genetics, Proto-Oncogene Proteins
- Abstract
In this study, we have isolated the genomic DNA clone encoding the murine JAK3 gene and determined its sequence. Partial genomic clones of the JAK1 and JAK2 genes encoding the tyrosine kinase domain were also isolated and compared with JAK3. The genomic structure of JAK3 consists of 23 exons. The exon/intron boundaries and the distribution of coding sequences within the exons of each of the three JAK genes were found to follow a similar pattern suggesting that various members of the JAK family have originated from a single primordial gene by duplication and the structure has been closely maintained through evolution. Using in situ hybridization and FISH analysis, we have mapped the JAK3 gene to human chromosome 19p13.1-p13.2.
- Published
- 1996
24. Transcriptional activation potential of normal and tumor-associated myb isoforms does not correlate with their ability to block GCSF-induced terminal differentiation of murine myeloid precursor cells.
- Author
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Patel G, Tantravahi R, Oh IH, and Reddy EP
- Subjects
- Animals, Cell Differentiation drug effects, Cell Differentiation physiology, Cell Line, Genetic Vectors, Granulocyte Colony-Stimulating Factor pharmacology, Hematopoietic Stem Cells drug effects, Hematopoietic Stem Cells physiology, Isomerism, Mice, Neoplasm Proteins genetics, Phenotype, Proto-Oncogene Proteins genetics, Proto-Oncogene Proteins c-myb, Retroviridae genetics, Trans-Activators genetics, Transformation, Genetic, Granulocyte Colony-Stimulating Factor antagonists & inhibitors, Hematopoietic Stem Cells cytology, Neoplasm Proteins physiology, Proto-Oncogene Proteins physiology, Trans-Activators physiology, Transcriptional Activation
- Abstract
The myb gene has been shown to be an important regulator of hematopoietic cell proliferation, differentiation and apoptosis. Activation of the myb gene into an oncogenic form has involved structural alterations to the coding sequences. Thus, the v-myb gene encoded by the Avian Myeloblastosis Virus, is truncated at both the 5' and 3' ends. Additionally, tumor cells containing rearrangements in the myb locus, such as the ABPL tumors or NFS60 tumor cell line have recently been shown to display a heterogeneity of structure. In this study, we examined the growth and differentiation properties of clonal cell lines derived from 32Dcl3 which harbor myb transgenes; derived from v-myb, and the ABPL-1, ABPL-2, ABPL-4 and NFS-60 cell lines. Retroviral vectors containing the appropriate myb cDNAs were produced, transfected into packaging cell lines, and the viruses were used to generate the 32D derivative cell clones. Abrogation of IL-3 dependence was never observed in any cell line. Expression of c-myb, ABPL-1-myb and ABPL-2-myb isoforms in 32D cells resulted in a block to their ability to terminally differentiate into granulocytes at the pro-myelocytic stage. However, expression of ABPL-4-myb or NFS60-myb in these cells failed to result in a similar effect. These cells differentiated into granulocytes in the presence of G-CSF, albeit more slowly than control 32Dcl3 cells. We also examined the ability of various Myb-isoforms to transactivate transcription of reporter genes containing Myb-binding elements in their promoter/enhancer sequences, to determine whether the phenotypic effects produced by these various isoforms correlate with their ability to transactivate transcription. Our results show that while v-myb and c-myb transactivated transcription equally well, the NFS60-myb exhibited the highest levels of transcriptional transactivation. The ABPL-1, ABPL-2 and ABPL-4-myb isoforms showed very low levels of transcriptional transactivation potential with the same reporter genes. These results suggest that the ability of various Myb-isoforms to transactivate transcription does not by itself correlate with their ability to induce a block to G-CSF-induced terminal differentiation of myeloid precursor cells.
- Published
- 1996
25. Murine myeloid leukemic cells with disrupted myb loci show splicing anomalies that account for heterogeneous sizes in myb proteins.
- Author
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Tantravahi R, Dudek H, Patel G, and Reddy EP
- Subjects
- Amino Acid Sequence, Base Sequence, Chromosome Mapping, Gene Rearrangement, Humans, Molecular Sequence Data, Polymerase Chain Reaction, Protein Biosynthesis, Proto-Oncogene Proteins c-myb, RNA, Messenger genetics, RNA, Messenger metabolism, Ribonucleases metabolism, Transcription, Genetic, Transcriptional Activation, Transformation, Genetic, Tumor Cells, Cultured, Leukemia, Myeloid genetics, Leukemia, Myeloid metabolism, Proto-Oncogene Proteins biosynthesis, Proto-Oncogene Proteins genetics, RNA Splicing, Trans-Activators biosynthesis, Trans-Activators genetics
- Abstract
The ABPL tumor cell lines represent a group of myeloid cell lines which contain an altered myb locus due to viral insertional mutagenesis within the third exon of c-myb. Immunoprecipitation analysis of the proteins produced in three ABPL lines revealed an interesting anomaly. Despite the invariant position of the virus integration event, the three ABPL tumor cell lines we examined (ABPL-1, ABPL-2 and ABPL-4) produced three different sized proteins. In this report, we examined the molecular basis for this protein size heterogeneity. Molecular cloning and sequence analysis of the cDNAs derived from the myb transcripts show that ABPL-1 tumor produces a tripartate mRNA containing sequences derived from the viral gag and env genes fused to the myb coding region. This results in the synthesis of a 74 kd protein. In the ABPL-2 tumor line, a gag-myb fusion protein is produced which is of 68 kd. In ABPL-4 cell line a gag-myb fusion protein is produced which contains an internal deletion of coding sequences derived from exons 13 and 14. This deletion results in the synthesis of a 59 kd protein in ABPL-4 tumor cell line. These observations were further confirmed by RNase protection assays which demonstrate the presence of aberrantly spliced mRNAs in ABPL-1 and ABPL-4 tumor cells but not in cells containing an undisrupted c-myb locus. In vitro translation and immuno-precipitation analysis of the cRNAs derived from the ABPL-1, ABPL-2 and ABPL-4 cDNAs show the synthesis of protein products that were identical to Myb proteins produced by these tumors in vivo. These results suggest that integration of Mo-MuLV within the c-myb locus not only results in deletions of the 5' end of the transcript but splicing aberrations within the encoded mRNA, which results in the synthesis of a heterogeneous array of proteins, not seen in normal hematopoietic cells.
- Published
- 1996
26. Temporal patterns of A-myb and B-myb gene expression during testis development.
- Author
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Latham KE, Litvin J, Orth JM, Patel B, Mettus R, and Reddy EP
- Subjects
- Animals, Cell Division physiology, DNA-Binding Proteins genetics, Down-Regulation, Female, Gene Expression, Male, Meiosis physiology, Mice, Mice, Inbred C57BL, Prophase physiology, Proto-Oncogene Proteins genetics, Proto-Oncogene Proteins c-myb, RNA, Messenger genetics, RNA, Messenger metabolism, Spermatocytes cytology, Spermatocytes metabolism, Spermatogonia cytology, Spermatogonia metabolism, Testis embryology, Trans-Activators genetics, Transcription Factors genetics, Cell Cycle Proteins, DNA-Binding Proteins biosynthesis, Proto-Oncogene Proteins biosynthesis, Spermatogenesis physiology, Testis growth & development, Testis metabolism, Trans-Activators biosynthesis, Transcription Factors biosynthesis
- Abstract
We recently reported the cloning and sequencing of the mouse A-myb proto-oncogene cDNA and the abundant expression of this mRNA primarily in the testis of adult mice. The A-myb mRNA is detectable by in situ hybridization specifically in the spermatogenic cells, and is downregulated during terminal differentiation. A low level of expression is observed in a few other tissues, including ovary, spleen and brain. We have extended those studies by examining A-myb and B-myb expression during testis development in the mouse. The A-myb and B-myb genes are both expressed in a cell- and stage-specific manner during testis development. The B-myb mRNA is expressed most highly in gonocytes of the fetal testis and in spermatogonia and early spermatocytes in the adult. B-myb expression decreases at day 18 post partum, coincident with the initial appearance of late pachytene spermatocytes. B-myb expression was also detectable in some interstitial cells of the fetal and adult testis. The A-myb mRNA was not detectable by in situ hybridization in fetal day 15.5 gonocytes but was detectable at a low abundance by RT-PCR in fetal and newborn mice. A-myb mRNA expression increased at post-natal day 10, when primary spermatocytes first appear. In the adult, the A-myb mRNA was expressed highly in a sub-population of spermatogonia and in primary spermatocytes, but was not detectable in spermatids. This expression of A-myb is consistent with the meiotic arrest that is observed in A-myb-deficient male mice. We conclude that B-myb may play a critical role in controlling the proliferation or differentiation of gonocytes and spermatogonia and possibly the somatic lineages as well, whereas A-myb is required for progression through the first meiotic prophase. These distinct roles for B-myb and A-myb during spermatogenesis may reflect distinct transactivation potentials of the two proteins. Further studies to determine the functions of A-myb and B-myb in the developing testis should improve our understanding of the molecular events associated with spermatogenesis and differentiation of the Sertoli and other somatic cell types of the testis.
- Published
- 1996
27. Identification of a novel Bcl-2 related gene, BRAG-1, in human glioma.
- Author
-
Das R, Reddy EP, Chatterjee D, and Andrews DW
- Subjects
- Amino Acid Sequence, Base Sequence, Gene Expression, Humans, Molecular Sequence Data, Sequence Analysis, DNA, Apoptosis genetics, Brain Neoplasms genetics, Genes genetics, Glioma genetics, Open Reading Frames genetics
- Abstract
Programmed cell death (apoptosis) plays a major role in embryogenesis, in mature organ homeostasis and in many disease states including cancer. Apoptosis occurs as an orderly cell-intrinsic suicide program regulated by a family of genes related to Bcl-2. Here, we describe the cloning and molecular characterization of a gene homologous to Bcl-2 from a human glioma. This gene named BRAG-1 (for brain-related apoptosis gene) has an open reading frame that encodes for a protein of 31 kDa sharing significant sequence homology with the Bcl-2 family of genes in the BH1 and BH2 regions. Northern blot analyses revealed that BRAG-1 is expressed in human gliomas as a 1.8 kb message. This gene, interestingly, was found to be expressed predominantly in normal human brain as a 4.5 kb transcript which is different in size from the message found in tumor tissues. These results suggest that BRAG-1 may be rearranged in human gliomas leading to its over-expression as a truncated transcript. Utilizing a bacterial expression vector, we produced BRAG-1 protein which was found to cross-react with a Bcl-2 monoclonal antibody, further suggesting structural and immunological similarity to Bcl-2.
- Published
- 1996
28. Transducers of life and death: TNF receptor superfamily and associated proteins.
- Author
-
Baker SJ and Reddy EP
- Subjects
- Animals, Apoptosis, Humans, Proteins physiology, TNF Receptor-Associated Factor 1, TNF Receptor-Associated Factor 2, fas Receptor physiology, Receptors, Tumor Necrosis Factor physiology, Signal Transduction
- Abstract
Signal transduction pathways which are initiated by members of the TNF superfamily utilize receptors which are devoid of intrinsic catalytic activity. Isolation and characterization of death domain (TNF-RI, Fas, TRADD, FADD/MORT-1, RIP) and TRAF domain-containing proteins (TRAF-1, TRAF-2, TRAF-3) have partially bridged a large molecular gap within one of several signaling pathways which originate at the plasma membrane and terminate in the nucleus. The ability of these two protein families to selectively dimerize and bind to related receptors allows them to govern diverse cellular responses which culminate in cellular proliferation, differentiation, effector functions, and apoptosis.
- Published
- 1996
29. B cell differentiation: role of E2A and Pax5/BSAP transcription factors.
- Author
-
Baker SJ and Reddy EP
- Subjects
- Animals, B-Lymphocytes cytology, Base Sequence, Cell Differentiation, Consensus Sequence, Enhancer Elements, Genetic, Gene Expression Regulation, Genes, Immunoglobulin, Genes, Switch, Helix-Loop-Helix Motifs, Hematopoiesis, Mice, Mice, Transgenic, Molecular Sequence Data, PAX5 Transcription Factor, Promoter Regions, Genetic, RNA, Messenger genetics, TCF Transcription Factors, Transcription Factor 7-Like 1 Protein, B-Lymphocytes physiology, DNA-Binding Proteins physiology, Nuclear Proteins physiology, Transcription Factors
- Abstract
Transcriptional regulation of lineage specific genes has the ability to dictate both the proliferative and differentiative potentials of a pluipotent precursor cell. The E2A and Pax5/BSAP genes encode transcription factors which bind to B cell specific promoters and enhancers and guide the development of immature, but committed cells into mature B lymphocytes which express and secrete immunoglobulins. In vitro analysis has consistently suggested that these proteins regulate distinct classes of genes during B cell differentiation; however, recent targeted gene disruption and transgene expression in mice has indicated that these genes may actually be components of a single regulatory mechanism which is essential for both B lymphocyte differentiation and proliferation.
- Published
- 1995
30. Cell cycle control in mammalian cells: role of cyclins, cyclin dependent kinases (CDKs), growth suppressor genes and cyclin-dependent kinase inhibitors (CKIs).
- Author
-
Graña X and Reddy EP
- Subjects
- Animals, Humans, Cell Cycle physiology, Cyclin-Dependent Kinases antagonists & inhibitors, Cyclin-Dependent Kinases physiology, Cyclins physiology, Enzyme Inhibitors pharmacology, Genes, Tumor Suppressor
- Abstract
All eukaryotic cells possess similar mechanisms to regulate the progression of the cell cycle. However, higher eukaryotes have evolved to respond to a large array of positive and negative signals with an intracellular or extracellular origin. These signals are eventually integrated by a conserved protein engine consisting of holoenzymes with kinase activity, which trigger crucial transitions during the cell cycle. In this review, the mechanisms by which the mammalian cell cycle engine integrates intracellular and extracellular signals of different nature are discussed.
- Published
- 1995
31. Anatomy of an integrated avian myeloblastosis provirus: structure and function.
- Author
-
Baluda MA and Reddy EP
- Subjects
- Amino Acid Sequence, Avian Myeloblastosis Virus genetics, Base Sequence, DNA, Viral, Genes, env, Genes, gag, Genes, pol, Genome, Viral, Molecular Sequence Data, Oncogene Proteins v-myb, Proviruses genetics, Retroviridae Proteins, Oncogenic genetics, Avian Myeloblastosis Virus physiology, Proviruses physiology
- Published
- 1994
32. Murine A-myb: evidence for differential splicing and tissue-specific expression.
- Author
-
Mettus RV, Litvin J, Wali A, Toscani A, Latham K, Hatton K, and Reddy EP
- Subjects
- Amino Acid Sequence, Animals, Cloning, Molecular, DNA, Complementary, Down-Regulation, Humans, Male, Mice, Molecular Sequence Data, Proto-Oncogene Proteins biosynthesis, Proto-Oncogene Proteins c-myb, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Spermatogenesis genetics, Tissue Distribution, Trans-Activators biosynthesis, Oncogenes, Proto-Oncogene Proteins genetics, RNA Splicing, Trans-Activators genetics
- Abstract
The myb gene family consists of three members, A-myb, B-myb and c-myb. The proteins encoded by these genes bind DNA in a sequence-specific manner and regulate transcription of target genes. In this communication, we report the nucleotide sequence of murine A-myb cDNA. This gene encodes a protein of 751 amino acids with an estimated molecular weight of 83 kDa. cDNA sequence analysis of multiple independent cDNA clones reveals the presence of alternatively spliced mRNAs that encode smaller proteins. Northern blot analysis of adult mouse tissue RNAs reveals A-myb expression predominantly in the testis, with very low levels of expression in the ovaries, spleen and brain. In situ hybridization analysis of adult mouse testis shows that this gene is expressed at high levels in type A spermatogonia (stem cells), and preleptotene and pachytene spermatocytes, with concomitant down-regulation of expression upon terminal differentiation of these cells into mature spermatozoa. This pattern of expression suggests that A-myb is involved in the control of proliferation and differentiation of spermatogonia. This potential function of A-myb in spermatogenesis is reminiscent of the role of c-myb in hematopoiesis.
- Published
- 1994
33. JAK3: a novel JAK kinase associated with terminal differentiation of hematopoietic cells.
- Author
-
Rane SG and Reddy EP
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Cell Differentiation, Cell Line, Cloning, Molecular, DNA, Complementary chemistry, DNA, Complementary isolation & purification, Gene Expression Regulation, Granulocyte Colony-Stimulating Factor pharmacology, Janus Kinase 2, Janus Kinase 3, Mice, Molecular Sequence Data, Polymerase Chain Reaction, Protein-Tyrosine Kinases chemistry, Protein-Tyrosine Kinases physiology, Hematopoietic Stem Cells physiology, Protein-Tyrosine Kinases genetics, Proto-Oncogene Proteins
- Abstract
The Janus Kinases (JAK) JAK1, JAK2, and TYK2 are protein tyrosine kinases which play a pivotal role in the signal transduction process mediated by cytokines. These kinases appear to transduce signals via their substrates which modulate programs of gene expression specific to the respective signals. It is becoming increasingly evident that certain cytokines such as Granulocyte Colony Stimulating Factor (GCSF) can transmit signals for both cellular proliferation and differentiation. It is at present unclear whether both of these signals are transmitted by the same JAK kinase or whether an entire family of such kinases are involved in this process. To determine if additional members of JAK kinase family exist, we designed a polymerase chain reaction based strategy which resulted in the identification of a new member of the JAK kinase family. This new kinase, which we have named JAK3 is encoded by a 4.3 kb mRNA transcript. Nucleotide sequence analysis of a full length cDNA derived from this mRNA revealed that it encodes an open reading frame of 3897 bp. The protein encoded by this mRNA contains the double catalytic domain characteristic of the JAK family kinases. The most striking difference between JAK3 and the other JAK kinases is the presence of two stretches of additional amino acid sequence of 147 and 28 residues which span between amino acid positions 322 to 469 and 632 to 660 respectively. Expression studies indicate that JAK3 is expressed at very low levels in immature hematopoietic cells, but its expression is dramatically up-regulated during terminal differentiation of these cells. These results suggest that JAK3 plays an important role in the differentiation of hematopoietic cells.
- Published
- 1994
34. The Myb oncogene product induces DNA-bending.
- Author
-
Saikumar P, Gabriel JL, and Reddy EP
- Subjects
- Base Sequence, DNA-Binding Proteins, Electrophoresis, Models, Molecular, Molecular Sequence Data, Protein Conformation, Proto-Oncogene Proteins c-myb, Trans-Activators, Nucleic Acid Conformation, Proto-Oncogene Proteins physiology
- Abstract
The nuclear oncogene v-myb and its cellular counterpart c-myb code for proteins that bind to DNA in a sequence specific manner and act as regulators of transcription. The Myb protein contains DNA binding and trans-regulatory domains which are important for its function. The DNA binding domain of Myb protein has been shown to contain three imperfectly conserved repeats of 50-52 amino acids that constitute the amino terminal end. In this communication, we show that Myb protein induces conformational change in DNA after protein-DNA complex formation. Circular permutation assays indicate that Myb protein induces DNA bending at the site of binding. Phasing analysis confirm the DNA bending and allowed the detection of relative orientation of bend. Myb proteins which comprise only DNA-binding domains either with three repeats or two repeats also bend DNA in the same orientation as the larger proteins with both DNA-binding and transactivating domains. However, the transactivating region seems to influence the magnitude of bend angle. We used molecular modeling to analyse the structure of Myb-DNA complex formation resulting in the bending of DNA. Data presented here show that Myb protein, like other transcriptional regulators, bends DNA upon binding allowing the interaction of regulatory elements.
- Published
- 1994
35. Ribozyme-mediated cleavage of the BCRABL oncogene transcript: in vitro cleavage of RNA and in vivo loss of P210 protein-kinase activity.
- Author
-
Shore SK, Nabissa PM, and Reddy EP
- Subjects
- Base Sequence, Cells, Cultured, Genetic Therapy, Genetic Vectors, Humans, Leukemia, Myelogenous, Chronic, BCR-ABL Positive therapy, Molecular Sequence Data, RNA, Catalytic genetics, Retroviridae genetics, Fusion Proteins, bcr-abl genetics, Oncogenes, RNA, Catalytic physiology, RNA, Messenger metabolism
- Abstract
The t(9,22) chromosomal translocation generating the Philadelphia chromosome and the BCRABL oncogene has been shown both cytogenetically and molecularly to be the etiologic event in chronic myelogenous leukemia (CML). We have designed a ribozyme to cleave the BCRABL mRNA by targeting a GUU triplet adjacent to the junction of the c-BCR and c-ABL fused genes. This ribozyme efficiently cleaved BCRABL RNA transcripts as demonstrated by in vitro cleavage reactions. To determine the effect of constitutive expression of the ribozyme on the elimination of the BCRABL gene product, the ribozyme cDNA sequence was inserted into different retroviral expression vectors. Introduction of the recombinant retroviruses into the CML blast crisis cell-line K562, resulted in the elimination of the P210 protein-kinase activity in several single cell clones infected with the ribozyme expression cassette. Therefore BCR-ABL specific ribozymes may provide a potential genetic therapy for CML.
- Published
- 1993
36. Alternative splicing of the chicken c-myb exon 9A.
- Author
-
Schuur ER, Dasgupta P, Reddy EP, Rabinovich JM, and Baluda MA
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Chickens, DNA chemistry, DNA genetics, Humans, Molecular Sequence Data, Proto-Oncogene Proteins c-myb, RNA, Messenger chemistry, RNA, Messenger genetics, Alternative Splicing, Exons, Proto-Oncogene Proteins genetics, Proto-Oncogenes
- Abstract
The c-myb gene products are thought to be regulators of cellular replication and of differentiation and heterogeneity may underlie their multiple functions. To investigate the possible existence of heterogeneity we have examined the chicken c-myb mRNAs by Northern blot analysis and polymerase chain reaction amplification of cDNAs (RT-PCR). Northern blot analysis with the c-myb cDNA clone pSG3, which contains the entire open reading frame (ORF) plus 500 base pairs of 3' untranslated sequences (Gerondakis & Bishop, 1986), and genomic probes revealed c-myb RNA species of 4.3 kb in addition to the major 4.0 kb species. The 4.3 kb c-myb RNA contained the alternatively spliced exon 9A which is highly conserved and has also been detected in a minor 4.3 kb alternatively spliced c-myb mRNA in murine and human cells. Sequencing of the avian exon 9A revealed 360 bp exon homologous to that found in murine and human mRNAs, which contains three highly conserved sequence regions shared by all three species. RT-PCR demonstrated usage of exon 9A in five hematopoietic tissues and revealed an additional splicing variant which used the 3' portion of exon 9A. Northern blot analysis using splice site-specific oligonucleotide probes spanning the two splice junctions between exon 9 and 9A revealed four additional c-myb RNAs of 4.4 kb, 2.2 kb, 2.0 kb and 1.4 kb.
- Published
- 1993
37. Mutational analysis of Max: role of basic, helix-loop-helix/leucine zipper domains in DNA binding, dimerization and regulation of Myc-mediated transcriptional activation.
- Author
-
Reddy CD, Dasgupta P, Saikumar P, Dudek H, Rauscher FJ 3rd, and Reddy EP
- Subjects
- Base Sequence, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, Basic-Leucine Zipper Transcription Factors, DNA-Binding Proteins genetics, Humans, Molecular Sequence Data, Repressor Proteins, Structure-Activity Relationship, DNA metabolism, DNA-Binding Proteins metabolism, Leucine Zippers, Mutation, Proto-Oncogene Proteins c-myc physiology, Transcription Factors, Transcriptional Activation
- Abstract
The Max protein forms a heterodimeric complex with the Myc family of proteins and binds to DNA in a sequence-specific manner. We investigated the role of the helix-loop-helix (HLH), leucine zipper (LZ) and basic domains of Max in protein complex formation, DNA-binding activity and transcriptional regulation. We mutagenized the basic, HLH and LZ domains of Max and studied the ability of the normal and mutant proteins to bind to DNA as both homo- and heterodimers and their ability to heterodimerize with Myc. Helix-1 and helix-2 regions of Max were found to be critical for homodimer formation and subsequent DNA binding, while the LZ was essential for heterodimer formation. In transient transfection assays the Myc protein functioned as a transcriptional activator while Max protein repressed the trans-activation observed with Myc.
- Published
- 1992
38. Large-scale molecular mapping of human c-myb locus: c-myb proto-oncogene is not involved in 6q- abnormalities of lymphoid tumors.
- Author
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Park JG and Reddy EP
- Subjects
- Blotting, Southern, Chromosomes, Human, Pair 6, Electrophoresis, Gel, Pulsed-Field, Humans, Methylation, Proto-Oncogene Mas, Restriction Mapping, Tumor Cells, Cultured, Chromosome Aberrations, Leukemia, Myelomonocytic, Acute genetics, Leukemia-Lymphoma, Adult T-Cell genetics, Lymphoma, Non-Hodgkin genetics, Oncogenes genetics
- Abstract
Chromosomal abnormalities in the 6q region have been observed frequently in several T-cell and myeloid leukemias. Interestingly, this region was found to contain three cellular oncogenes, c-myb, c-fyn and c-ros. Several of the tumors that exhibit 6q- abnormalities have also been found to express high levels of c-myb and, in some cases, amplification of the c-myb gene, leading to the suggestion that this gene could lie in proximity to the deletions observed in these tumors. To determine if c-myb gene activation is associated with 6q- abnormalities, we developed a megabase map of the human c-myb locus using pulsed-field gel electrophoresis. We then examined the occurrence of abnormalities near the c-myb gene in several hematopoietic tumor cell lines containing well-characterized 6q- abnormalities. Our results show that no rearrangements or deletions occur within a region of 1.0 Mbp of the c-myb locus in these cell lines. However, several of the cell lines exhibited differential and partial methylation patterns which seem to be prevalent amongst different cell lines.
- Published
- 1992
39. Oncogenes and the Nobel Prize.
- Author
-
Vande Woude GF Jr and Reddy EP
- Subjects
- Animals, Humans, Nobel Prize, Oncogenes
- Published
- 1990
40. Nucleotide sequence of chicken c-myb complementary DNA and implications for myb oncogene activation.
- Author
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Rosson D and Reddy EP
- Subjects
- Amino Acid Sequence, Animals, Avian Myeloblastosis Virus genetics, Base Sequence, Chickens, Cloning, Molecular, Genes, Viral, Proto-Oncogene Mas, Proto-Oncogenes, RNA, Messenger genetics, Cell Transformation, Neoplastic, DNA analysis, Oncogenes
- Abstract
Avian myeloblastosis virus (AMV), like other acute transforming viruses, arose by recombination between its helper virus and host cellular sequences. The latter sequences, termed v-myb, are responsible for the oncogenic properties of the virus. AMV causes acute myeloblastic leukaemia in chickens and transforms a specific class of haematopoietic cells in vitro, but does not induce morphological transformation of cultured fibroblasts, suggesting that only a restricted target-cell population is responsive to its transforming gene product. The normal cellular counterpart of v-myb, c-myb, is highly conserved and is present in all vertebrate and some invertebrate species examined. DNA rearrangements and altered expression of the myb oncogene have been reported in mouse lymphoid tumours and human myeloid and colon tumours. The mechanism of activation of the cellular proto-oncogenes is thought to involve the structural alteration of the coding regions that result in either the synthesis of an altered gene product or the enhanced expression of a proto-oncogene caused by alterations in its regulatory elements. To distinguish between these two mechanisms, we have cloned and sequenced the chicken c-myb complementary DNA and compared it with that of v-myb sequences. We demonstrate that during the transduction of the cellular sequences and/or viral passage a substantial portion of the coding region of the c-myb gene has been lost from both the 5' and 3' ends, resulting in the generation of a truncated gene product that mediates the transforming function of the virus.
- Published
- 1986
- Full Text
- View/download PDF
41. A cDNA sequence encoding a rabbit heavy chain variable region of the VHa2 allotype showing homologies with human heavy chain sequences.
- Author
-
Bernstein KE, Reddy EP, Alexander CB, and Mage RG
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Humans, Rabbits, Species Specificity, Binding Sites, Antibody genetics, DNA analysis, Immunoglobulin Allotypes genetics, Immunoglobulin Heavy Chains genetics, Immunoglobulin Variable Region genetics
- Published
- 1982
- Full Text
- View/download PDF
42. Acquisition of transforming properties by alternative point mutations within c-bas/has human proto-oncogene.
- Author
-
Yuasa Y, Srivastava SK, Dunn CY, Rhim JS, Reddy EP, and Aaronson SA
- Subjects
- Base Sequence, Cell Line, Cloning, Molecular, Glutamine genetics, Humans, Leucine genetics, Lung Neoplasms, Proto-Oncogene Mas, Urinary Bladder Neoplasms genetics, Cell Transformation, Neoplastic, Mutation, Oncogenes
- Abstract
The transforming gene of a human lung carcinoma-derived cell line, Hs242, has been cloned in biologically active form, and identified as c-bas/has (otherwise known as c-Ha-ras). The genetic lesion responsible for the transforming activity of the Hs242 oncogene has been localized to a point mutation in the second exon which results in the substitution of leucine for glutamine as amino acid 61 of the predicted protein. No changes were observed in the first exon, the region of c-bas/has in which a point mutation is responsible for activation of the T24 and EJ bladder carcinoma oncogenes.
- Published
- 1983
- Full Text
- View/download PDF
43. Molecular cloning of the breakpoint region on chromosome 6 in cutaneous malignant melanoma: evidence for deletion in the c-myb locus and translocation of a segment of chromosome 12.
- Author
-
Dasgupta P, Linnenbach AJ, Giaccia AJ, Stamato TD, and Reddy EP
- Subjects
- Base Sequence, Cloning, Molecular, Gene Rearrangement, Humans, Molecular Sequence Data, Proto-Oncogene Proteins c-myb, Tumor Cells, Cultured, Chromosome Deletion, Chromosomes, Human, Pair 12, Chromosomes, Human, Pair 6, Melanoma genetics, Proto-Oncogene Proteins genetics, Proto-Oncogenes, Skin Neoplasms genetics, Translocation, Genetic
- Abstract
Nonrandom involvement of chromosome 6 in cutaneous malignant melanoma have been noted by several investigators. Recently an alteration in the c-myb locus (6q22-23) has been identified by Southern analysis in the WM983A cell line which was derived from a primary melanoma of the vertical growth phase. In the present study, the nature of this rearrangement in the WM983A cell line has been further characterized by molecular cloning and nucleotide sequence analysis of the break-point region in the c-myb locus. The results of this investigation demonstrate that the rearrangement in the 6q22-23 region results in deletion of the 3'-end of the c-myb locus with the concomitant translocation of a portion of chromosome 12 to chromosome 6.
- Published
- 1989
44. Effect of Abelson murine leukemia virus on granulocytic differentiation and interleukin-3 dependence of a murine progenitor cell line.
- Author
-
Rovera G, Valtieri M, Mavilio F, and Reddy EP
- Subjects
- Animals, Cell Differentiation drug effects, Cell Division drug effects, Cell Line, Cell Transformation, Viral, Colony-Stimulating Factors pharmacology, Granulocyte Colony-Stimulating Factor, Mice, Abelson murine leukemia virus genetics, Granulocytes cytology, Interleukin-3 physiology, Leukemia Virus, Murine genetics, Oncogenes
- Abstract
The murine diploid hematopoietic cell line 32D Cl3 strictly requires interleukin-3 (IL-3) for proliferation. When 32D Cl3 cells are transferred to IL-3-free medium which contains recombinant human granulocyte colony stimulating factor (rhG-CSF), the cell number increases four- to five-fold, and after 14 days the whole cell population is differentiated into morphologically normal and myeloperoxidase- and lactoferrin-positive metamyelocytes and granulocytes. Infection with Abelson murine leukemia virus (A-MuLV) of 32D Cl3 cells growing in the presence of IL-3 induces, within 2 weeks, the appearance of cells that are IL-3-independent for growth. The latter cells lack myeloid, T and B cell markers, and are unable to differentiate, even in the presence of very high doses of rhG-CSF. However, once the 32D Cl3 cells have been exposed to G-CSF, they become resistant to the transforming effects of A-MuLV as judged by the appearance of the IL-3-independent clones. These findings suggest that the ability of Abelson virus to transform immature progenitor cells is due to interference of the v-abl gene product with the mechanisms that control the commitment of the cells to differentiate.
- Published
- 1987
45. Transformation of NIH3T3 cells by A-MuLV proviral DNA: effect of plasmid linearization and carrier DNA on transformation efficiency.
- Author
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Shore SK and Reddy EP
- Subjects
- Animals, Cells, Cultured, DNA genetics, Mice, Mice, Inbred Strains, Salmon, Transfection, Abelson murine leukemia virus genetics, Cell Transformation, Neoplastic, DNA, Viral genetics, Leukemia Virus, Murine genetics, Plasmids, Proviruses genetics
- Abstract
We have optimized the conditions for efficient NIH3T3 focus formation by calcium phosphate transfection of proviral Abelson-murine leukemia virus (A-MuLV) plasmid DNA. Linearized pA-MuLV, P120 or P160 strains, when transfected with calf thymus carrier DNA, will produce 40-50 foci/100 ng pA-MuLV without co-transfecting Moloney-murine leukemia virus (Mo-MuLV) plasmid DNA.
- Published
- 1989
46. Immunoglobulin synthesis and gene rearrangements in lymphoid cells transformed by replication-competent Rauscher murine leukemia virus: transformation of B cells at various stages of differentiation.
- Author
-
Balachandran R, Reddy EP, Dunn CY, Aaronson SA, and Swan DC
- Subjects
- Animals, B-Lymphocytes immunology, Cell Differentiation, Cell Line, Cell Transformation, Neoplastic, Genes, Immunoglobulins genetics, Mice, Oncogenes, Virus Replication, B-Lymphocytes microbiology, Cell Transformation, Viral, Immunoglobulins biosynthesis, Rauscher Virus genetics
- Abstract
Lymphoid cells transformed by Rauscher murine leukemia virus (R-MuLV) belonged to the B cell lineages. One group of cells exhibited Fc receptors but completely lacked immunoglobulin mu heavy and kappa light chains. The majority of the cells resemble pre-B type. They displayed mu chains but kappa chains were completely absent. Very rarely certain cells synthesized both mu and kappa chains. Based on the presence of Fc receptors and IgM synthesis the cells transformed by R-MuLV belonged to three B cell developmental stages. These cells were tested for immunoglobulin gene rearrangements using JH and CK probes. DNA from cell lines without any detectable levels of IgM mu exhibited embryonic as well as rearranged JH genes, whereas cells expressing IgM possess, in addition, productive and non-productive light chain gene rearrangements. The most terminally differentiated cell possesses JH and CK rearrangement associated with the synthesis of mu and kappa chains. Presumably the cells with rearranged JH and CK genes without immunoglobulin synthesis represent a developmental transition. We conclude that cells transformed by R-MuLV belonged to five step-wise compartments of B cell development. Our findings implicate definite sequential events of immunoglobulin gene rearrangement and expression during B cell development.
- Published
- 1984
- Full Text
- View/download PDF
47. Alteration of growth and differentiation factors response by Kirsten and Harvey sarcoma viruses in the IL-3-dependent murine hematopoietic cell line 32D C13(G).
- Author
-
Mavilio F, Kreider BL, Valtieri M, Naso G, Shirsat N, Venturelli D, Reddy EP, and Rovera G
- Subjects
- Butyrates pharmacology, Butyric Acid, Cell Division, Dimethyl Sulfoxide pharmacology, Gene Expression Regulation, Genes, ras, Granulocyte Colony-Stimulating Factor, Granulocytes cytology, Hematopoiesis, Interleukin-3 pharmacology, Lactoferrin genetics, Peroxidase genetics, Tretinoin pharmacology, Cell Differentiation drug effects, Cell Transformation, Viral, Colony-Stimulating Factors pharmacology, Harvey murine sarcoma virus, Hematopoietic Stem Cells cytology, Kirsten murine sarcoma virus, Sarcoma Viruses, Murine
- Abstract
32D C13(G) is an interleukin 3(IL3)-dependent non-tumorigenic murine hematopoietic cell line which undergoes terminal differentiation into granulocytes when exposed to granulocytic colony stimulating factor (G-CSF). Infections of 32D C13(G) cells with either Kirsten rat sarcoma virus or Balb murine sarcoma virus, both containing a v-ras oncogene, generates clones that can permanently grow in G-CSF without differentiation. 32D-Ki-ras cells show a heterogeneous morphology ranging from the promyelocytic to the myelocytic stage of differentiation, and express high levels of both myeloperoxidase (MPO) and lactoferrin (LF) mRNA. 32D-Ha-ras cells show a more immature phenotype and express MPO but no LF mRNA. The apparent differentiation block of both 32D Ki-ras and 32D Ha ras can be reversed by treatment with the chemical inducers retinoic acid, sodium butyrate or dimethylsulphoxide, which leads to terminal differentiation into granulocytes. When 32D-Ki-ras and 32D-Ha-ras cells are cultured in medium containing IL-3 they become adherent and express some monocyte-macrophage markers. Upon prolonged exposure to IL3, 32D-Ki-ras, but not 32D-Ha-ras, resume suspension growth. Both 32D-Ki-ras and 32D-Ha-ras rapidly die if grown in chemically defined medium in the absence of any growth factor and are non-tumorigenic in immunosuppressed mice. These findings indicate that ras activation may interfere with the normal response to growth and differentiation factors in cells of the granulocytic lineage. These alterations may represent a critical, although non-sufficient, step in leukemogenesis.
- Published
- 1989
48. Murine myeloid leukemias with aberrant myb loci show heterogeneous expression of novel myb proteins.
- Author
-
Dudek H and Reddy EP
- Subjects
- Animals, Antibodies, Cell Line, Cell Nucleus enzymology, Cytoplasm enzymology, Epitopes analysis, Isoenzymes genetics, Leukemia, Experimental enzymology, Mice, Proto-Oncogene Proteins immunology, Proto-Oncogene Proteins c-myb, RNA Splicing, RNA, Messenger genetics, Leukemia, Experimental genetics, Protein-Tyrosine Kinases genetics, Proto-Oncogene Proteins genetics, Proto-Oncogenes
- Abstract
Using a panel of anti-myb antibodies, we have examined the c-myb proteins present in the NFS-60 and ABPL-1, ABPL-2, and ABPL-4 tumor cell lines, all of which have an altered myb locus due to viral insertional mutagenesis. As predicted from previous DNA and RNA analysis, NFS-60 cells produce myb protein with a truncated C-terminus and a normal N-terminus. The three ABPL tumor cell lines studied here were previously shown to have undergone similar rearrangements towards the 5' end of myb locus and were expected to synthesize identical myb RNAs and proteins. These cell lines were found to produce myb proteins with N-terminal modifications. Surprisingly, however, the three cell lines were found to synthesize a heterogenous array of myb proteins of different sizes. These observations suggest that oncogenic activation of myb in ABPL tumors may involve additional changes in the coding region in addition to those occurring at the N-terminus.
- Published
- 1989
49. Identification of alternatively spliced transcripts for human c-myb: molecular cloning and sequence analysis of human c-myb exon 9A sequences.
- Author
-
Dasgupta P and Reddy EP
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Cell Line, Drosophila genetics, Humans, Mice, Molecular Sequence Data, Proto-Oncogene Proteins c-myb, Restriction Mapping, Sequence Homology, Nucleic Acid, Cloning, Molecular, Exons, Protein-Tyrosine Kinases genetics, Proto-Oncogene Proteins genetics, Proto-Oncogenes, RNA Splicing, RNA, Messenger genetics, Transcription, Genetic
- Abstract
The murine c-myb gene has been recently shown to code for two protein products of 75kd and 89kd. The 89kd protein appears to be generated from an alternatively spliced mRNA which contains an additional stretch of 363 bases between exons 9 and 10. In this communication, we have examined whether similar alternatively spliced mRNAs of c-myb occur in human cells. Human c-myb exon 9A has been identified and sequenced in a cDNA clone (ML5) generated from the acute myeloid leukemic cell line ML-2. This alternatively spliced exon of c-myb has been found to contain the same number of nucleotides (363bp) as the corresponding mouse exon. Between murine and human exon 9A sequences, 81% sequence homology was found at the DNA level, while the homology at the predicted amino acid level was found to be 73%. A stretch of 14 amino acid residues at the junction of exons 9A and 10 have been found to be conserved between Drosophila and human sequences indicating that this region might perform an essential biological function which was deemed necessary through evolution.
- Published
- 1989
50. A point mutation is responsible for the acquisition of transforming properties by the T24 human bladder carcinoma oncogene.
- Author
-
Reddy EP, Reynolds RK, Santos E, and Barbacid M
- Subjects
- Amino Acid Sequence, Base Sequence, Cell Line, Gene Expression Regulation, Genes, Viral, Humans, Neoplasm Proteins genetics, Retroviridae genetics, Mutation, Oncogenes, Urinary Bladder Neoplasms genetics
- Abstract
The genetic change that leads to the activation of the oncogene in T24 human bladder carcinoma cells is shown to be a single point mutation of guanosine into thymidine. This substitution results in the incorporation of valine instead of glycine as the twelfth amino acid residue of the T24 oncogene-encoded p21 protein. Thus, a single amino acid substitution appears to be sufficient to confer transforming properties on the gene product of the T24 human bladder carcinoma oncogene.
- Published
- 1982
- Full Text
- View/download PDF
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