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Start Over Topic liquid chromatography-mass spectrometry Publisher oxford university press (oup)
2 results

1. Determination of Aromatic Amines Released from Azo Dyes in Paper Packaging by Liquid Chromatography-Tandem Mass Spectrometry

Author

Liu Shanshan, Huimin Deng, Yang Fei, Zhonghao Li, Gangling Tang, Fan Ziyan, Zhaoyang Bian, and Wang Ying

Subjects
Paper, 02 engineering and technology, Tandem mass spectrometry, 01 natural sciences, Analytical Chemistry, Sodium dithionite, chemistry.chemical_compound, Tandem Mass Spectrometry, Liquid chromatography–mass spectrometry, Environmental Chemistry, Amines, Coloring Agents, Pharmacology, Chromatography, 010401 analytical chemistry, Extraction (chemistry), Food Packaging, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Food packaging, Chromatographic separation, chemistry, Mass spectrum, 0210 nano-technology, Citric acid, Azo Compounds, Agronomy and Crop Science, Chromatography, Liquid, Food Science
Abstract

An LC-tandem MS (LC–MS/MS) method for the determination of 21 kinds of carcinogenic aromatic amines released from azo dyes in food wrappers was used in this research. Sodium dithionite was added to a citric acid buffer medium to reduce and decompose possible azo dyes. The extract was analyzed after liquid–liquid extraction (LLE) and dispersive SPE (d-SPE). The conditions for chromatographic separation, mass spectrum, LLE, and d-SPE were optimized. Under optimal conditions, the LOD was in the range of 0.13–0.35 mg/kg and LOQ in the range of 0.38–1.05 mg/kg, with the addition of standard recoveries of most aromatic amines being ≥80% and RSDs ≤10%. The recoveries for 2,4-diaminotoluene and 2,4-diaminoanisole were significantly lower, being ≤40%. The method was successfully used to analyze 30 practical samples, and the results showed that it is user-friendly, with high sensitivity, rapid control, and low matrix interference.

Published
2016

2. A New, Validated Wipe-Sampling Procedure Coupled to LC-MS Analysis for the Simultaneous Determination of 5-Fluorouracil, Doxorubicin and Cyclophosphamide in Surface Contamination

Author

Christelle Audeval, Christelle Percheron, Christine Bobin-Dubigeon, Pierre Leynia, Marie Amiand, Sophie Rochard, and Jean-Marie Bard

Subjects
Paper, Quality Control, Antimetabolites, Antineoplastic, Health, Toxicology and Mutagenesis, Toxicology, Mass Spectrometry, Analytical Chemistry, Limit of Detection, Liquid chromatography–mass spectrometry, Occupational Exposure, medicine, Environmental Chemistry, Doxorubicin, Antineoplastic Agents, Alkylating, Cyclophosphamide, Chromatography, High Pressure Liquid, Detection limit, Antibiotics, Antineoplastic, Chemical Health and Safety, Chromatography, Filter paper, Chemistry, Extraction (chemistry), technology, industry, and agriculture, Reproducibility of Results, Reference Standards, Contamination, Solutions, Fluorouracil, Calibration, Equipment Contamination, Indicators and Reagents, Wipe sampling, medicine.drug
Abstract

A wipe-sampling procedure followed by a simple liquid chromatography-mass spectrometry method was developed and validated for the simultaneous quantification of three cytotoxic drugs [5-fluorouracil (5FU), doxorubicin and cyclophosphamide (CP)] for the determination of surface contamination. After a solid-phase extraction procedure with wiping filter paper, the separation was performed within 30 min using a gradient mobile phase. The method was validated according to the recommendations of the US Food and Drug Administration. Wiping was performed using Whatman(®) filter paper on different surfaces such as stainless steel, polypropylene and glass. The method was linear, between 10 and 500 ng per wiping sample (i.e., 0.1-5 ng/cm(2)) for 5FU and doxorubicin, and between 1-100 ng per wiping sample (i.e., 0.01-1 ng/cm(2)) for CP. The lower limits of detection and quantification were 5 and 10 ng per wiping sample for 5FU and doxorubicin, and 0.5 and 1 ng per wiping sample for CP. This new sensitive methodology for surface contamination studies was successfully applied on commercial vials and different places in a cancer research hospital. This approach is particularly suitable to assess the risk of occupational exposure to cytotoxic drugs and to optimize the cleaning process, especially for the most toxic molecule studied, CP.

Published
2013