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The article discusses the results of a study that examines the non-radio-labeled probe-based colony blotting assay detection method for rapid enumeration of Salmonella serovars in seafood and water samples. The probe-based technique using a digoxigenin-based labeling assay is compared with the quantitative polymerase chain reaction (PCR) assay. the Salmonella in spiked seafood and water samples was successfully quantified by the assay.

Aims: This study aimed to determine the effect of packaging [air, modified atmosphere (MA)] on microbial growth, sensory and chemical parameters and also on shelf life of fresh pearl spot ( Etroplus suratensis Bloch) and on the selection of microbial association. Methods and Results: Fresh pearl spot (whole, gutted) were packaged under both 100% air and MAs (40%CO2/60% O2, 50%CO2/50%O2, 60% CO2/40%O2, 70% CO2/30% O2 and 40% CO2/30% O2/30% N2) and stored at 0°C. Microbial growth (counts of total aerobic bacteria, H2S–producing bacteria, Lactic acid bacteria, Brochothrix thermosphacta, yeast and mould), chemical spoilage indicators (pH, total volatile basic nitrogen) and sensory characteristics were monitored. Microbial changes in Pearl spot packed under 100% air and 40% CO2/30%O2/30% N2 were similar. The total volatile basic nitrogen values increased, but the values never exceeded the acceptability limit of 25 mg 100 g−1. Conclusions: MA 60% CO2 : 40%O2 was found to be better with a shelf life of 21 days whereas air stored samples had a shelf-life of 12–14 days only. Significance and Impact of the Study: Storage of pearl spot under MAs 60% CO2 : 40%O2 is a promising method to extend shelf-life. Longer shelf life expands the market potential of pearl spot and reduces waste during distribution and retail display. [ABSTRACT FROM AUTHOR]