1. Expression of CD82 in human trophoblast and its role in trophoblast invasion
- Author
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Tan Yi, Wen-Ping Luo, Junjie Lu, Dongmei Tan, and Qian Zhang
- Subjects
Embryology ,Immunofluorescence ,Fluorescent Antibody Technique ,lcsh:Medicine ,Matrix metalloproteinase ,Kangai-1 Protein ,Biochemistry ,RNA interference ,Cell Movement ,Pregnancy ,Molecular Cell Biology ,Signaling in Cellular Processes ,lcsh:Science ,reproductive and urinary physiology ,Multidisciplinary ,Reverse Transcriptase Polymerase Chain Reaction ,Decidua ,Obstetrics and Gynecology ,Cell Differentiation ,Cell migration ,Immunohistochemistry ,Trophoblasts ,Cell biology ,Nucleic acids ,medicine.anatomical_structure ,embryonic structures ,Medicine ,Female ,Cell Movement Signaling ,Research Article ,Signal Transduction ,Blotting, Western ,Immunology ,In Vitro Techniques ,Biology ,Cell Line ,Placenta ,medicine ,Humans ,Cell Proliferation ,Matrigel ,Cell growth ,lcsh:R ,Proteins ,Trophoblast ,Molecular biology ,Transmembrane Proteins ,Cell culture ,Immunologic Techniques ,RNA ,lcsh:Q ,Developmental Biology - Abstract
Background Well-controlled trophoblast invasion at maternal-fetal interface is a critical event for the normal development of placenta. CD82 is a member of transmembrane 4 superfamily, which showed important role in inhibiting tumor cell invasion and migration. We surmised that CD82 are participates in trophoblast differentiation during placenta development. Methodology/Principal Findings CD82 was found to be strongly expressed in human first trimester placental villous and extravillous trophoblast cells as well as in trophoblast cell lines. To investigate whether CD82 plays a role in trophoblast invasion and migration, we further utilized human villous explants culture model on matrigel and invasion/migration assay of trophoblast cell line HTR8/SVneo. CD82 siRNA significantly promoted outgrowth of villous explants in vitro (P
- Published
- 2012