1. Loss of ARL13 impedes BBSome-dependent cargo export from Chlamydomonas cilia.
- Author
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Dai J, Zhang G, Alkhofash RA, Mekonnen B, Saravanan S, Xue B, Fan ZC, Betleja E, Cole DG, Liu P, and Lechtreck K
- Subjects
- Cilia metabolism, Dyneins metabolism, Flagella metabolism, Kinesins, Membrane Proteins metabolism, Protein Transport, Chlamydomonas genetics, Chlamydomonas metabolism, GTP Phosphohydrolases metabolism, Phospholipase D metabolism
- Abstract
The GTPase Arl13b participates in ciliary protein transport, but its contribution to intraflagellar transport (IFT), the main motor-based protein shuttle of cilia, remains largely unknown. Chlamydomonas arl13 mutant cilia were characterized by both abnormal reduction and accumulation of select membrane-associated proteins. With respect to the latter, a similar set of proteins including phospholipase D (PLD) also accumulated in BBSome-deficient cilia. IFT and BBSome traffic were apparently normal in arl13. However, transport of PLD, which in control cells moves by BBSome-dependent IFT, was impaired in arl13, causing PLD to accumulate in cilia. ARL13 only rarely and transiently traveled by IFT, indicating that it is not a co-migrating adapter securing PLD to IFT trains. In conclusion, the loss of Chlamydomonas ARL13 impedes BBSome-dependent protein transport, resulting in overlapping biochemical defects in arl13 and bbs mutant cilia., (© 2022 Dai et al.)
- Published
- 2022
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