1. A turn-on fluorescence aptasensor based on carbon dots for sensitive detection of adenosine.
- Author
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Shen, Xin, Xu, Lei, Zhu, Wanying, Li, Bingzhi, Hong, Junli, and Zhou, Xuemin
- Subjects
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ADENOSINES , *FLUORESCENCE resonance energy transfer , *SINGLE-stranded DNA - Abstract
In this paper, a novel turn-on fluorescence aptasensor has been designed for adenosine detection based on fluorescence resonance energy transfer (FRET) from single-stranded DNA labeled carbon dots (ssDNA-CDs) to aptamer modified gold nanoparticles (aptamer-AuNPs). In the absence of adenosine, the fluorescence of ssDNA-CDs was quenched by aptamer-AuNPs via the formation of an aptamer–ssDNA duplex. The introduced adenosine competed to displace ssDNA-CDs by specifically binding to the aptamer, resulting in the recovery of the quenched fluorescence of ssDNA-CDs. Under optimized conditions, the increase in fluorescence intensity was proportional to the concentration of adenosine with a linear range of 10–500 nM and allowed a limit of detection as low as 4.2 nM. In addition, this method was successfully applied to adenosine determination in human serum samples with satisfactory results. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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