1. In vivo and in vitro studies using Clonorchis sinensis adult-derived total protein (CsTP) on cellular function and inflammatory effect in mouse and cell model.
- Author
-
Shang M, Sun H, Wu Y, Gong Y, Tang Z, Meng F, He L, Yu X, Huang Y, and Li X
- Subjects
- Animals, Cell Movement physiology, Cell Proliferation physiology, Clonorchiasis parasitology, Clonorchis sinensis genetics, Cytokines metabolism, Foodborne Diseases parasitology, Humans, Hydrogen Peroxide metabolism, Inflammation pathology, Interferon-alpha metabolism, Liver Cirrhosis parasitology, Mice, Microtubule-Associated Proteins metabolism, Proto-Oncogene Proteins c-akt metabolism, Proto-Oncogene Proteins c-bcl-2 biosynthesis, RNA-Binding Proteins metabolism, Signal Transduction, TOR Serine-Threonine Kinases metabolism, Tumor Necrosis Factor-alpha metabolism, Up-Regulation, Apoptosis physiology, Clonorchiasis pathology, Clonorchis sinensis pathogenicity, Liver Cirrhosis pathology, Protozoan Proteins metabolism
- Abstract
Clonorchis sinensis (C. sinensis) can induce a food-borne parasitic disease (clonorchiasis). Numerous studies have analyzed functional proteins, immunologic factors, pro-inflammatory cytokines, and cell signaling transduction that promote the development of clonorchiasis. In a previous study, it was shown that C. sinensis adult-derived total protein (CsTP) might be involved in the pathogenesis and development of liver fibrosis via bringing about Th2 immune response. In the present study, further investigation of CsTP on cellular function and inflammatory effect in vitro and in vivo has been elicited. CsTP induced inflammation and autophagy as evidenced by upregulation of TNF-α, IFN-γ, and autophagic markers LC3B and P62. Exposed to CsTP upregulated the antiapoptotic gene Bcl-2 expression, diminished the apoptosis induced by H
2 O2 , but promoted the proliferation and migration of LX-2 cells in proper concentration range. Additionally, the protein levels of p-AKT and p-mTOR were repressed in response to CsTP, suggesting a correlation of blocking the activation of mTOR/AKT signaling pathway. These results revealed that CsTP might exacerbate hepatic pathological changes by regulating cell proliferation, apoptosis, autophagy, and inflammation in the liver and LX-2 cells. Some effects might be partially involved in the mTOR and AKT pathways.- Published
- 2020
- Full Text
- View/download PDF