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Strain DM2021935 T representing a novel Acinetobacter species was isolated from a spoiled bath lotion in Guangdong, China. Based on 16S rRNA gene phylogenetic analysis, strain DM2021935 T was closely related to 'Acinetobacter thutiue' VNH17 T , Acinetobacter junii CIP 64.5  T , and Acinetobacter tibetensis Y-23  T . Cells of strain DM2021935 T were Gram-stain-negative, non-spore-forming, strictly aerobic, catalase-positive, oxidase-negative, α-hemolytic, and non-motile. Strain DM2021935 T exhibited growth in 1-3% (w/v) NaCl at temperatures ranging from 4 to 37 °C and tolerated pH levels from 6.0 to 8.0. The predominant fatty acids in strain DM2021935 T are C 12:0 , C 16:0 , C 18:1 ω9c, and summed feature 3. Polar lipid profiles included glycolipids, phospholipids, phosphatidylethanolamine, and phosphatidyl-N-methylethanolamine. The identified respiratory quinones were ubiquinone Q-8 and Q-9. The genomic size of DM2021935 T comprised 4.15 Mb, consisting of one chromosome (3,827,633 bp) and two plasmids (241,357 and 83,010 bp). The G + C content was 41.8%. The average nucleotide identity, average amino acid identity, and digital DNA-DNA hybridization values between strain DM2021935 T and phylogenetically related type strains were below the species delineation thresholds (72.2-95.4, 53.1-87.0, and 20.4-66.4%, respectively). AntiSMASH analysis identified four gene clusters: non-ribosomal peptide synthetase, non-alpha poly-amino group acids, YcaO cyclodehydratase, and aryl polyene biosynthesis. Based on genotypic data, strain DM2021935 T represents a novel species within the genus Acinetobacter. The proposed name for the novel species is Acinetobacter corruptisaponis sp. nov. (type strain DM2021935 T  = KCTC 92772  T  = GDMCC 1.3703  T )., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

An actinobacterium, designated as SYSU T00001 T , was isolated from a tidal flat sediment sample from Guangdong province, China. Cells were Gram-stain-positive, aerobic, motile and short rod-shaped. Colonies on marine agar 2216 were smooth, yellow-pigmented, and circular with low convexity. The isolate was able to grow at the temperature range 4-37 °C (optimum 30 °C), at pH 4.0-10.0 (optimum 7.0) and in the presence of 0-10% (w/v) NaCl. The major menaquinones were MK-11 and MK-10. The cell wall contained alanine, glutamic acid, lysine and ornithine. The major fatty acids were C 19:0 cyclo ω8c (35.7%) and anteiso C 15:0 (26.0%). The polar lipids consisted of one diphosphatidyl glycerol, one unidentified glycolipid and one unknown lipid. Whole genome sequencing of strain SYSU T00001 T revealed 2,837,702 bp with a DNA G + C content of 67.8%. Phylogenetic analyses clearly demonstrated that strain SYSU T00001 T belonged to the genus Salinibacterium, and the highest 16S rRNA gene similarity to Salinibacterium hongtaonis 194 T (97.8%). The ANI and dDDH values of strain SYSU T00001 T relative to Salinibacterium hongtaonis 194 T were 74.5% and 19.5%, respectively. According to our data, strain SYSU T00001 T represents a novel species of the genus Salinibacterium, for which the name Salinibacterium sedimenticola sp. nov. is proposed, the type strain is SYSU T00001 T (= GDMCC 1.3283 T  = KCTC 49758 T )., (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

A Gram-stain-negative, aerobic, short rod-shaped strain, designated as SYSU D60009 T , was isolated from a dry sandy soil sample collected from the Gurbantunggut Desert in Xinjiang, northwest China. Strain SYSU D60009 T was observed to grow at 15-42 °C (optimum at 37 °C), pH 4.0-10.0 (optimum at 7.0), and with 0-0.5% (w/v) NaCl (optimum, 0%). The strain grew well on R2A agar, and colonies were smooth, white-pigmented, and circular with low convexity. The polar lipids consisted of phosphatidylethanolamine, aminolipid, aminophospholipid, and unknown lipids. The major cellular fatty acid (> 10%) was C 16:0 and the predominant respiratory quinone was Q-10. Whole genome sequencing of strain SYSU D60009 T revealed 6,132,710 bp with a DNA G + C content of 63.6%. The ANI and dDDH values of strains SYSU D60009 T to Dongia mobilis CGMCC 1.7660  T were 72.8% and 19.0%, respectively. Based on the phenotypic, phylogenetic, and chemotaxonomic properties, strain SYSU D60009 T represents a novel species of the genus Dongia, for which the name Dongia deserti sp. nov. is proposed, the type strain is SYSU D60009 T (= CGMCC 1.16441  T  = KCTC 52790  T )., (© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Egicoccus halophilus EGI 80432 T , a halotolerant bacterium isolated from a saline-alkaline soil, belongs to a member of the class Nitriliruptoria, which exhibits high adaptability to salt environments. At present, the detailed knowledge of the salinity adaptation strategies of Nitriliruptoria was limited except for one research by using comparative genomics analysis. Here, we investigated the salinity adaptation mechanism of E. halophilus EGI 80432 T by comparative physiological and transcriptomic analyses. The results of physiological analyses showed that trehalose and glutamate were accumulated by salt stress and showed the maximum at moderate salinity condition. Furthermore, the contents of histidine, threonine, proline, and ectoine were increased with increasing salt concentration. We found that both 0% and 9% NaCl conditions resulted in increased expressions of genes involved in carbohydrate and energy metabolisms, but negatively affected the Na + efflux, iron, and molybdate transport. Moreover, the high salt condition led to enhancement of transcription of genes required for the synthesis of compatible solutes, e.g., glutamate, histidine, threonine, proline, and ectoine, which agree with the results of physiological analyses. The above results revealed that E. halophilus EGI 80432 T increased inorganic ions uptake and accumulated trehalose and glutamate in response to moderate salinity condition, while the salinity adaptation strategy was changed from a "salt-in-cytoplasm" strategy to a "compatible solute" strategy under high salinity condition. The findings in this study would promote further studies in salt tolerance molecular mechanism of Nitriliruptoria and provide a theoretical support for E. halophilus EGI 80432 T 's application in ecological restoration.Key Points• Salt stress affected gene expressions responsible for carbohydrate and energy metabolisms of E. halophilus EGI 8042 T .• E. halophilus EGI 80432 T significantly accumulated compatible solutes under salt stress.• E. halophilus EGI 80432 T adopted a "compatible solute" strategy to withstand high salt stress.