1. T cell receptor signaling strength establishes the chemotactic properties of effector CD8+ T cells that control tissue-residency.
- Author
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Abdelbary, Mahmoud, Hobbs, Samuel J., Gibbs, James S., Yewdell, Jonathan W., and Nolz, Jeffrey C.
- Subjects
T cells ,CELL communication ,T cell receptors ,INTERFERON receptors ,TISSUE differentiation ,IMMUNE response - Abstract
Tissue-resident memory (T
RM ) CD8+ T cells are largely derived from recently activated effector T cells, but the mechanisms that control the extent of TRM differentiation within tissue microenvironments remain unresolved. Here, using an IFNγ-YFP reporter system to identify CD8+ T cells executing antigen-dependent effector functions, we define the transcriptional consequences and functional mechanisms controlled by TCR-signaling strength that occur within the skin during viral infection to promote TRM differentiation. TCR-signaling both enhances CXCR6-mediated migration and suppresses migration toward sphingosine-1-phosphate, indicating the programming of a 'chemotactic switch' following secondary antigen encounter within non-lymphoid tissues. Blimp1 was identified as the critical target of TCR re-stimulation that is necessary to establish this chemotactic switch and for TRM differentiation to efficiently occur. Collectively, our findings show that access to antigen presentation and strength of TCR-signaling required for Blimp1 expression establishes the chemotactic properties of effector CD8+ T cells to promote residency within non-lymphoid tissues. CD8+ T cells are found within peripheral tissues including the skin. Here, the authors use an interferon-gamma reporter system and viruses expressing agonistic peptides of varying affinities to investigate how T cell receptor signaling strength changes the chemotactic properties of effector CD8+ T cells to promote tissue-residency. [ABSTRACT FROM AUTHOR]- Published
- 2023
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