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In this study, the antioxidant and antimicrobial potentials of the ethanol extract of the aerial parts of Lepidium spinosum Ard., which is distributed in many regions of the world, were determined. The aerial parts of the plant were dried, powdered and extracted with ethanol. Total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) of the plant extract were determined using Rel Assay Diagnostics kits. Antimicrobial activities of the plant extract were determined against standard bacterial and fungal strains by agar dilution method. As a result of the studies, the TAS value of the plant extract was determined as 4.550±0.132, the TOS value as 12.610±0.221, and the OSI value as 0.277±0.007. In addition, it has been found to be effective at 25-200 µg/mL against standard bacterial strains and 100-200 µg/mL concentrations against fungal strains. As a result, it was determined that L. spinosum used in our study could be a natural antioxidant and antimicrobial source. In this context, it is thought that natural material may be used in pharmacological designs.

Plants have been used for centuries to treat various diseases. In this study, Marrubium globosum Montbret & Aucher ex Benth. plant was used as a material. The extract of the plant was extracted with the help of soxhlet device using ethanol, which is a good polar solvent, and Rel Assay kits were used to determine the oxidant and antioxidant levels in the plant extract. Antifungal and antibacterial activities of M. globossum were tested against standard bacteria and fungus strains by agar dilution method. As a result of the analysis, TAS value of plant extract was determined as 7.677±0.231, TOS value as 12.387±0.083 and OSI value as 0.162±0.004. In this context, it has been observed that the plant has an important antioxidant potential. In addition, the plant extract was found to be effective against test microorganisms at 50-200 μg/mL extract concentrations. As a result, it has been determined that M. globossum can be a natural antioxidant and antimicrobial source.

Many people in different parts of the world benefit from alternative medicine in the treatment and prevention of diseases. Plants are among the important natural materials used in alternative medicine. In this study, the antioxidant and antimicrobial potential of Datura stramonium L. was determined. Ethanol extracts of the plant's flower parts were obtained in the soxhlet device. The antioxidant and oxidant potential of the plant extract was determined using Rel Assay TAS and TOS kits. Antimicrobial activity was tested by the agar dilution method. The TAS value of the plant extract was 7.559±0.224 mmol/L, the TOS value was 10.711±0.243 μmol/L, and the OSI value was 0.142±0.002. It was determined that the plant extract was effective against bacteria and fungus strains at 100-400 μg/mL concentration. As a result, it was determined in our study that D. stramonium can be a natural antioxidant and antimicrobial source.

In this study, antioxidant, oxidant and oxidative stress index of Thymbra spicata L. collected from Duhok (Iraq) region were determined. The aerial parts of the plant were extracted with ethanol. Rel Assay Diagnostics kits were used for detect TAS, TOS and OSI values. As a result of the studies, it was determined that TAS value of plant extract was determined 8.399±0.102, TOS value was 6.530±0.115 and OSI value was 0.078±0.001. As a result, it was determined that T. spicata has high antioxidant potential and in this context, can be used as a natural resource.

One of the alternative therapeutic methods is herbal medicine. Leontice leontopetalum belongs to Berberidaceae family. The aim of study was investigated the extract of LL on human pancreatic beta cell-treated with STZ. Materials and methods: The human pancreatic beta cell (1.1B4) line was used the current study. LL’s extracts (1, 10, 100, and 1000 ug/ml) were supplemented in media for twenty-four hours and/or after STZ treatment (10 and 20 mM). Cells survivals (MTT), cells proliferation were shown by using xCelligence. Insulin content and releasing were measured at 1.1, 8.4 and 16.7 mM glucose concentrations. Results: The result of MTT was shown that cell survival was decreased, based on dose-dependent. When looked at xCelligence results, cell proliferation in STZ groups and the lowest and highest concentrations of LL were attenuated in a dose-dependent manner. Also, cotreatments of LL and STZ were decreased as well. The result of insulin-releasing on glucose induction was shown that STZ concentration gave rise to reduce insulin content at low and high glucose levels. Also, co-treatment of LL and STZ attenuated insulin content based on dose. Conclusion: It was considered that LL treatment led to increased insulin realizing, resulting from decreasing insulin content in diabetic beta cells, but decrease cell survival.

The present study aimed to determine antioxidant and antimicrobial activities of Salvia multicaulis Vahl plant collected in Gaziantep province, Turkey. Total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) were determined using Rel Assay kits. Antimicrobial activity was determined with modified agar dilution method. The findings demonstrated that S. multicaulis had high antioxidant activity. However, it is recommended to avoid excessive consumption of the plant due to high OSI. It was also determined that plant extracts possessed antimicrobial potential. As a result, it was determined that the plant can be used as a natural antioxidant and antimicrobial resource.