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2 results on '"Dionysios Liveris"'

1. Non‐thermal atmospheric plasma treatment of onychomycosis in an in vitro human nail model

Author

Raj K. Tiwari, Marc Zemel, Dionysios Liveris, Sarnath Singh, Jan Geliebter, Gary Friedman, Jeffry M. Bulson, Sin Park, and Irina L. Derkatch

Subjects
0301 basic medicine, medicine.medical_specialty, Plasma Gases, 030106 microbiology, C. albicans, Dermatology, cold atmospheric plasma, dielectric barrier discharge, In vitro model, Trichophyton mentagrophytes, 030207 dermatology & venereal diseases, 03 medical and health sciences, 0302 clinical medicine, Tinea, Trichophyton, Candida albicans, Onychomycosis, Cadaver, Confidence Intervals, medicine, Non thermal atmospheric plasma, Humans, pulsed electric field, Foot Dermatoses, Dose-Response Relationship, Drug, biology, business.industry, fungus, Candidiasis, Original Articles, General Medicine, Nail plate, biology.organism_classification, In vitro, Infectious Diseases, medicine.anatomical_structure, non‐thermal atmospheric plasma, Nail (anatomy), Original Article, business
Abstract

Background Onychomycosis affects almost 6% of the world population. Topical azoles and systemic antifungal agents are of low efficacy and can have undesirable side effects. An effective, non-invasive therapy for onychomycosis is an unmet clinical need. Objective Determine the efficacy threshold of non-thermal atmospheric plasma (NTAP) to treat onychomycosis in an in vitro model. Methods A novel toe/nail-plate model using cadaver nails and agarose media inoculated with Candida albicans was exposed to a range of NTAP doses. Results Direct exposure of C albicans and Trichophyton mentagrophytes to 12 minutes of NTAP results in complete killing at doses of 39 and 15 kPulses, respectively. Onset of reduced viability of C albicans to NTAP treatment through the nail plate occurs at 64 kPulses with 10× and 100× reduction at 212 and 550 kPulses, respectively. Conclusions NTAP is an effective, non-invasive therapeutic approach to onychomycosis that should be evaluated in a clinical setting.

Published
2019

2. Molecular Typing ofBorrelia burgdorferi

Author

Gabriele Margos, P. K. Mukherjee, Guiqing Wang, Ira Schwartz, Sabrina Jungnick, and Dionysios Liveris

Subjects
Spirochaetaceae, Polymerase Chain Reaction, Microbiology, Article, Lyme disease, Virology, Borrelia, medicine, Typing, Borrelia burgdorferi, biology, Molecular epidemiology, General Medicine, bacterial infections and mycoses, biology.organism_classification, medicine.disease, Bacterial Typing Techniques, LYME, Molecular Typing, RNA, Bacterial, RNA, Ribosomal, Multilocus sequence typing, Parasitology, Polymorphism, Restriction Fragment Length, Bacterial Outer Membrane Proteins
Abstract

Borrelia burgdorferi sensu lato is a group of spirochetes belonging to the genus Borrelia in the family of Spirochaetaceae. The spirochete is transmitted between reservoirs and hosts by ticks of the family Ixodidae. Infection with B. burgdorferi in humans causes Lyme disease or Lyme borreliosis. Currently, 20 Lyme disease-associated Borrelia species and more than 20 relapsing fever-associated Borrelia species have been described. Identification and differentiation of different Borrelia species and strains is largely dependent on analyses of their genetic characteristics. A variety of molecular techniques have been described for Borrelia isolate speciation, molecular epidemiology, and pathogenicity studies. In this unit, we focus on three basic protocols, PCR-RFLP-based typing of the rrs-rrlA and rrfA-rrlB ribosomal spacer, ospC typing, and MLST. These protocols can be employed alone or in combination for characterization of B. burgdorferi isolates or directly on uncultivated organisms in ticks, mammalian host reservoirs, and human clinical specimens.

Published
2014

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