13 results on '"Teh Ying Chou"'
Search Results
2. Genomic profiling with whole‐exome sequencing revealed distinct mutations and novel pathways in Asian melanoma
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Yu‐Jen Chiu, Hui‐Ying Weng, Yen‐Yu Lin, Yung‐Feng Lin, Yi‐Chen Yeh, Chern‐Kang Perng, Hsu Ma, Shih‐Feng Tsai, and Teh‐Ying Chou
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Skin Neoplasms ,Exome Sequencing ,Mutation ,Humans ,Genomics ,Dermatology ,General Medicine ,Melanoma - Abstract
The clinical characteristics of malignant melanoma are highly variable between patient populations of different ethnicities. To explore the underlining genetic variations, we reviewed the clinical data of 242 malignant melanoma cases from Taiwan and among them submitted formalin-fixed paraffin-embedded tissue samples from 37 patients for whole-exome sequencing to identify the mutational signatures, tumor mutation burden and specific gene mutations. The genomic profiles and clinical outcomes were compared with the information derived from the publicly available TCGA and TGEN databases. Mutation signature 12 was the dominant signature in Taiwanese patients and represented approximately 45% of the mutation signatures observed. In contrast, mutation signature 7 was the most prominent among cases available in the TCGA database. Common gene mutations found in the TCGA melanoma dataset were not frequently found in melanomas from Taiwanese patients. There were a significant number of specific gene mutations that exclusively occurred in acral subtype but not in non-acral subtype melanomas, and vice versa. While certain common mutations form a shared core of genetic features, there appear to be specific genetic pathways that are involved in the occurrence of melanomas that grow in non-UV-exposed areas. Our findings have shed light on the tumorigenesis pathways involved in malignant melanoma.
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- 2022
3. Primary pulmonary hepatoid carcinoma: Report of a case and review of the literature
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Shiou-Fu Lin, Wen-Hu Hsu, and Teh-Ying Chou
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Alpha-fetoprotein ,Hepatoid carcinoma ,Lungs ,Medicine (General) ,R5-920 - Abstract
Hepatoid carcinoma is a rare malignancy defined as extrahepatic primary alpha-fetoprotein-producing carcinoma morphologically resembling hepatocellular carcinoma. It is extremely rare in the lungs, with ambiguous pathological descriptions and variable prognosis. Herein, we present the case of a 66-year-old man with a primary pulmonary hepatoid carcinoma in his right upper lung who received complete curative surgical resection and adjuvant chemotherapy. No signs of recurrence or distant metastasis have been observed for 57 months postoperation. In addition, the literature is reviewed and the pathological diagnostic pitfalls are discussed.
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- 2013
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4. Programmed death‐ligand 1 (PD‐L1)/thyroid transcription factor‐1 double immunohistochemical staining facilitates scoring of tumor PD‐L1 expression in cytopathology specimens from lung adenocarcinoma patients
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Hsiang-Ling Ho, Yen-Yu Lin, Jen-Fan Hang, Teh Ying Chou, Li-Ya Lin, and Chia-Hung Lin
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Adult ,Male ,Cancer Research ,Pathology ,medicine.medical_specialty ,Lung Neoplasms ,Thyroid Nuclear Factor 1 ,030209 endocrinology & metabolism ,Adenocarcinoma ,Stain ,B7-H1 Antigen ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,Aged ,Retrospective Studies ,Aged, 80 and over ,business.industry ,Thyroid ,Middle Aged ,medicine.disease ,Immunohistochemistry ,Immune checkpoint ,Staining ,medicine.anatomical_structure ,Oncology ,Cytopathology ,030220 oncology & carcinogenesis ,Female ,Histopathology ,business - Abstract
BACKGROUND Immune checkpoint inhibitor therapy has revolutionized lung adenocarcinoma therapy. Treatment with antibodies against the immune checkpoint molecules programmed death-1 (PD-1) and programmed death-ligand 1 (PD-L1) can induce a durable response in a subset of patients. Immunohistochemistry characterization of tumor PD-L1 expression using either a histopathology specimen or a cytopathology specimen has been shown to correlate with treatment response. However, the current practice relies on pathologists' visual estimation of tumor PD-L1 staining, which can be variable in certain conditions. Highlighting tumor cells via double immunostaining with PD-L1 and thyroid transcription factor-1 (TTF-1) may improve estimation accuracy. METHODS We performed PD-L1 single staining and PD-L1/TTF-1 double staining in 42 pairs of cytopathology and histopathology specimens from lung adenocarcinoma patients. An experienced pathologist visually estimated PD-L1 expression in each case and placed tumor PD-L1 expression into 1 of 3 categories
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- 2020
5. Decreasing cytokeratin 17 expression in head and neck cancer predicts nodal metastasis and poor prognosis: The first evidence
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E.‐S. Xu, Muh Hwa Yang, Teh Ying Chou, T.‐Z. Hwang, T.‐T. Yang, C.‐Y. Liu, C.‐T. Hsu, and K.‐W. Liu
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0301 basic medicine ,Tissue microarray ,business.industry ,Head and neck cancer ,medicine.disease ,Head and neck squamous-cell carcinoma ,Metastasis ,03 medical and health sciences ,Cytokeratin ,030104 developmental biology ,0302 clinical medicine ,Otorhinolaryngology ,030220 oncology & carcinogenesis ,Cancer cell ,Carcinoma ,medicine ,Cancer research ,Immunohistochemistry ,business - Abstract
OBJECTIVES Cytokeratins (CKs) are mainly expressed in epithelial carcinomas and are valuable for making diagnoses and identifying metastatic status. Changes in the expression of individual CKs in certain carcinoma may be relevant to establishing a prognosis. However, the prognostic significance of CKs in head and neck squamous cell carcinoma (HNSCC) remains elusive. Herein, we investigated the diverse and unique expression patterns of Cytokeratin 13 (CK13) and Cytokeratin 17 (CK17) and assessed the role of CK17 as a predictor for HNSCC metastasis and prognosis. METHODS CK13 and CK17 expressions were evaluated using immunohistochemical tissue microarray (TMA) analysis with 106 patients of HNSCC. To clarify the characterisation of CK17 expression with respect to its ability in predicting metastatic disease, an in vitro study of cells migration/invasion assays was conducted. Furthermore, the correlation of CK17 expression to clinicopathologic variables and prognosis was analyzed using a serial statistical method. RESULTS CK13 was predominately expressed in non-cancerous tissues and was lost in HNSCC. Decreasing expression of CK17 correlated with cancerous cell migration and invasion (P
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- 2018
6. Molecular diagnostic algorithm for epidermal growth factor receptor mutation detection in Asian lung adenocarcinomas: Comprehensive analyses of 445 Taiwanese patients with immunohistochemistry, PCR-direct sequencing and Scorpion/ARMS methods
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Teh Ying Chou, Hsiang-Ling Ho, Yu-Ting Chuang, Hsiu-Hsun Ma, Fu-Pang Chang, Yi-Chun Chang-Chien, Li-Rung Liao, and Kun-Yang Lin
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Pulmonary and Respiratory Medicine ,Lung Neoplasms ,Taiwan ,Adenocarcinoma ,medicine.disease_cause ,Polymerase Chain Reaction ,Sensitivity and Specificity ,law.invention ,Exon ,Asian People ,Antibody Specificity ,law ,Humans ,Point Mutation ,Medicine ,Genetic Testing ,Epidermal growth factor receptor ,Polymerase chain reaction ,Mutation ,biology ,business.industry ,Point mutation ,Exons ,medicine.disease ,Immunohistochemistry ,ErbB Receptors ,Molecular Diagnostic Techniques ,biology.protein ,business ,Tyrosine kinase ,Algorithm ,Algorithms ,Gene Deletion - Abstract
Background and objective Therapeutic responses of lung adenocarcinoma patients to tyrosine kinase inhibitors (TKIs) of epidermal growth factor receptor (EGFR) are closely associated with activating mutations within the EGFR tyrosine kinase domain. Screening activating EGFR mutations prior to selection for therapeutic strategy has been considered extremely valuable for clinical management of lung adenocarcinoma patients in Asian countries including Taiwan, where the EGFR mutation rate is higher than in the rest of the world. Currently there is no consensus on the method of choice to assess EGFR mutations in tumour tissue. Methods We enrolled 445 lung adenocarcinoma patients for analysis of tumour EGFR mutations using polymerase chain reaction (PCR)-direct sequencing, scorpion/amplified refractory mutation system (ARMS) technology and immunohistochemistry with mutation-specific antibodies. Results Two hundred forty-five patients (245/445; 55%) were found to harbour activating EGFR mutations using PCR-direct sequencing method, with a majority of patients (233/245; 95%) carrying exon 19 deletion or p.L858R point mutations. One hundred three of 200 patients were negative for EGFR mutations from PCR-direct sequencing were further analysed using Scorpion/ARMS technology. Up to 30% of the PCR-direct sequencing negative patients turned out to be positive in the Scorpion/ARMS EGFR mutation tests. For immunohistochemistry analysis of EGFR mutations, the p.E746_A750del specific antibody showed a sensitivity of 57% and a specificity of 100% for exon 19 deletions while the p.L858R point mutation specific antibody showed a sensitivity of 68% and a specificity of 95%. Conclusions Based on this study, we proposed an algorithm for comprehensive and efficient testing of EGFR mutations on lung adenocarcinoma patients in Asia.
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- 2013
7. Overexpression of T-LAK cell-originated protein kinase predicts poor prognosis in patients with stage I lung adenocarcinoma
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Yu-Lun Kuo, Jin Mei Lai, Teh Ying Chou, Pei Jung Lu, Hui Chuan Cheng, Yi Chen Yeh, Jung Jyh Hung, Yu Lin Hsu, Yu Chung Wu, Kuan-Yu Chen, and Di Cing Wei
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Adult ,Male ,Cancer Research ,Pathology ,medicine.medical_specialty ,Lung Neoplasms ,Microarray ,T-Lymphocytes ,Adenocarcinoma of Lung ,Adenocarcinoma ,Protein Serine-Threonine Kinases ,medicine.disease_cause ,Recurrence ,Biomarkers, Tumor ,medicine ,Humans ,Killer Cells, Lymphokine-Activated ,Lung cancer ,Clonogenic assay ,Aged ,Aged, 80 and over ,Mitogen-Activated Protein Kinase Kinases ,Lung ,business.industry ,Cancer ,Original Articles ,General Medicine ,Middle Aged ,Prognosis ,medicine.disease ,medicine.anatomical_structure ,Oncology ,Cancer research ,Immunohistochemistry ,Female ,Carcinogenesis ,business - Abstract
Tumor recurrence is the most common cause of disease failure after surgical resection in early‐stage lung adenocarcinoma. Identification of clinically relevant prognostic markers could help to predict patients with high risk of disease recurrence. A meta‐analysis of available lung adenocarcinoma microarray datasets revealed that T‐LAK cell‐originated protein kinase (TOPK), a serine/threonine protein kinase, is overexpressed in lung cancer. Using stable cell lines with overexpression or knockdown of TOPK, we have shown that TOPK can promote cell migration, invasion, and clonogenic activity in lung cancer cells, suggesting its crucial role in lung tumorigenesis. To evaluate the prognostic value of TOPK expression in resected stage I lung adenocarcinoma, a retrospective analysis of 203 patients diagnosed with pathological stage I lung adenocarcinoma was carried out to examine the expression of TOPK by immunohistochemistry (IHC). The prognostic significance of TOPK overexpression was examined. Overexpression of TOPK (IHC score >3) was detected in 67.0% of patients, and these patients were more frequently characterized with disease recurrence and angiolymphatic invasion. Using multivariate analysis, patient age (>65 years old; P = 0.002) and TOPK overexpression (IHC score >3; P 3; P = 0.005) also significantly predicted a reduced time to recurrence in the patients. Our results indicate that overexpression of TOPK could predetermine the metastatic capability of tumors and could serve as a significant prognostic predictor of shortened overall survival and time to recurrence. (Cancer Sci 2012; 103: 731–738)
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- 2012
8. The total number of resected lymph node is not a prognostic factor for recurrence in esophageal squamous cell carcinoma patients undergone transthoracic esophagectomy
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Teh Ying Chou, Po-Kuei Hsu, Bing-Yen Wang, Wen-Hu Hsu, Chien-Sheng Huang, and Yu Chung Wu
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Oncology ,medicine.medical_specialty ,Prognostic factor ,Multivariate analysis ,business.industry ,Proportional hazards model ,General Medicine ,Transthoracic esophagectomy ,Esophageal cancer ,medicine.disease ,Esophageal squamous cell carcinoma ,medicine.anatomical_structure ,Internal medicine ,medicine ,Surgery ,Lymph ,business ,Lymph node - Abstract
Background The total number of resected lymph nodes (TLN) has been shown to predict survival in esophageal cancer, but its relationship with recurrence has been rarely reported. We aim to study the prognostic factors in esophageal squamous cell carcinoma (ESCC) patients, with a particular focus on the role of TLN. Methods Two hundred sixty-eight ESCC patients who underwent transthoracic esophagectomy were selected for the study. A Cox regression model was used to identify prognostic factors. Results Recurrence occurred in 115 of 268 patients. The median time to recurrence was 10 months (range, 1–58). The recurrence-free survival at 1, 3, and 5 years was 62.3%, 32.1%, and 28.5%, respectively. Multivariate analysis identified age (P = 0.001), N stage (N1–3 vs. N0, P = 0.001), tumor length (P = 0.019), and development of recurrence (P
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- 2011
9. Oestrogen-induced epithelial-mesenchymal transition of endometrial epithelial cells contributes to the development of adenomyosis
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Peng-Hui Wang, Ming Shyen Yen, Kuan Chong Chao, Nae Fang Twu, Yen Ni Liu, Teh Ying Chou, Hsin Yang Li, Muh Hwa Yang, Yi Jen Chen, and Chi Hung Huang
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medicine.medical_specialty ,medicine.drug_class ,Endometriosis ,Myometrium ,Biology ,Endometrium ,medicine.disease ,Pathology and Forensic Medicine ,Transplantation ,Endocrinology ,medicine.anatomical_structure ,Estrogen ,Internal medicine ,medicine ,Cancer research ,Adenomyosis ,Raloxifene ,Epithelial–mesenchymal transition ,skin and connective tissue diseases ,medicine.drug - Abstract
Adenomyosis is an oestrogen-dependent disease caused by a downward extension of the endometrium into the uterine myometrium. Epithelial-mesenchymal transition (EMT) endows cells with migratory and invasive properties and can be induced by oestrogen. We hypothesized that oestrogen-induced EMT is critical in the pathogenesis of adenomyosis. We first investigated whether EMT occurred in adenomyotic lesions and whether it correlated with serum 17β-oestradiol (E2) levels. Immunohistochemistry was performed on adenomyotic lesions and corresponding eutopic endometrium samples from women with adenomyosis. Endometria from women without endometrial disorders were used as a control. In the epithelial component of adenomyotic lesions, vimentin expression was up-regulated and E-cadherin expression was down-regulated compared to the eutopic endometrium, suggesting that EMT occurs in adenomyosis. In adenomyosis, the serum E2 level was negatively correlated with E-cadherin expression in the epithelial components of the eutopic endometrium and adenomyotic lesions, suggesting the involvement of oestrogen-induced EMT in endometrial cells. In oestrogen receptor-positive Ishikawa endometrial epithelial cells, oestrogen induced a morphological change to a fibroblast-like phenotype, a shift from epithelial marker expression to mesenchymal marker expression, increased migration and invasion, and up-regulation of the EMT regulator Slug. Raloxifene, a selective oestrogen receptor modulator, abrogated these effects. To determine the role of oestrogen-induced EMT in the implantation of ectopic endometrium, we xenotransplanted eutopic endometrium or adenomyotic lesions from adenomyosis patients into ovariectomized SCID mice. The implantation of endometrium was oestrogen-dependent and was suppressed by raloxifene. Collectively, these data highlight the crucial role of oestrogen-induced EMT in the development of adenomyosis and suggest that raloxifene may be a potential therapeutic agent for adenomyosis patients.
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- 2010
10. Pulmonary adenocarcinoma with microcystic histology and intratumoral heterogeneity of EGFR gene polymorphism
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Yi-Chen Yeh and Teh Ying Chou
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medicine.medical_specialty ,Pathology ,Histology ,Lung ,Cancer ,Anatomical pathology ,General Medicine ,Biology ,medicine.disease ,Pathology and Forensic Medicine ,medicine.anatomical_structure ,medicine ,biology.protein ,Adenocarcinoma ,Gene polymorphism ,Epidermal growth factor receptor ,Laser capture microdissection - Abstract
Yeh Y-C & Chou T-Y (2010) Histopathology 57, 112–120 Pulmonary adenocarcinoma with microcystic histology and intratumoral heterogeneity of EGFR gene polymorphism Aims: To study the clinicopathological features of pulmonary adenocarcinomas with microcystic histology. Materials and results: Two cases of pulmonary adenocarcinoma exhibiting a unique microcystic growth pattern were analysed. The 20-mm tumour of a 59-year-old woman showed a predominantly complex microcystic architecture with a focal papillary configuration, whereas the 15-mm tumour of another 46-year-old woman displayed a solely microcystic growth pattern. The first tumour was found to have metastasized to lymph nodes at surgery and, 4 years later, to the chest wall. The second tumour did not metastasize or recur over a follow-up period of 12 years. The tumour cells were immunoreactive with thyroid transcription factor-1 (TTF-1), which helped to differentiate them from salivary gland-type tumours. No mutations of epidermal growth factor receptor (EGFR) or K-ras genes were detected. However, facilitated by laser microdissection, a Q787Q polymorphism was found in the microcystic component but not in the papillary component. To our knowledge, this is the first report to illustrate intratumoral heterogeneity of EGFR gene polymorphism among different growth patterns within the same tumour. Conclusions: Pulmonary microcystic adenocarcinoma has a variable clinical outcome. The microcystic tumour cells are immunoreactive with TTF-1 and may harbour polymorphisms of the EGFR gene.
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- 2010
11. Extension of poly‐N‐acetyllactosamine chain in human cancer cell lines is under src regulation
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Hsuan-Chen Liu, Kuo-Yuan Hwa, and Teh-Ying Chou
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Poly-N-acetyllactosamine ,Chain (algebraic topology) ,Cell culture ,Chemistry ,Genetics ,Extension (predicate logic) ,Molecular Biology ,Biochemistry ,Molecular biology ,Human cancer ,Biotechnology ,Proto-oncogene tyrosine-protein kinase Src - Published
- 2012
12. ChemInform Abstract: Ubiquitous and Temporal Glycosylation of Nuclear and Cytoplasmic Proteins
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Kenneth D. Greis, R. C. Cole, Elizabeth P. Roquemore, Diane M. Snow, Lisa K. Kreppel, Melissa A. Blomberg, Bradley K. Hayes, Man-shiow Jiang, Teh-Ying Chou, Gerald W. Hart, and L.-Y. D. Dong
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chemistry.chemical_compound ,Glycosylation ,Chemistry ,Cytoplasm ,General Medicine ,Molecular biology - Abstract
Gerzrldk& K C I I I W ~ ~ 11. Greis, I,.-Y. Dermis I h g , Melissa A. Bloinherg Teh-Yirig Chou, MatiSliiow Jiatg Llizaheth I? I ~ ~ I ~ I I I ~ ~ c , loris M. Snow, Lisa K. Kreppel, Itoberl C. Cole atid Bradley K. Hayes. Dept. Biocheni. & Molecular Genetics, University of Alabama at Birmingham, UAB Station, 1918 University Avenue, Birmingham, AL 35294-0005;Phone: (205) 934-4753; FAX: (205) 975-6685; Internet: GWHART@BMG.BHS.UAB.EDU.
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- 2010
13. Efficacy of different platforms in detecting EGFR mutations using cerebrospinal fluid cell‐free DNA from non‐small‐cell lung cancer patients with leptomeningeal metastases
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Chi‐Lu Chiang, Hsiang‐Ling Ho, Yi‐Chen Yeh, Cheng‐Chia Lee, Hsu‐Ching Huang, Chia‐I Shen, Yung‐Hung Luo, Yuh‐Min Chen, Chao‐Hua Chiu, and Teh‐Ying Chou
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cell‐free DNA ,cerebrospinal fluid ,epidermal growth factor receptor ,next‐generation sequencing ,non‐small‐cell lung cancer ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Cell‐free tumor DNA (ctDNA) obtained through liquid biopsy is useful for the molecular analysis of advanced non‐small‐cell lung cancer (NSCLC). Few studies have directly compared analysis platforms in terms of their diagnostic performance in analyzing ctDNA obtained from the cerebrospinal fluid (CSF) of patients with leptomeningeal metastasis (LM). Methods We prospectively analyzed patients with epidermal growth factor receptor (EGFR)‐mutant NSCLC who were subjected to CSF analysis for suspected LM. To detect EGFR mutations, CSF ctDNA was analyzed using the cobas EGFR Mutation Test and droplet digital polymerase chain reaction (ddPCR). CSF samples from osimertinib‐refractory patients with LM were also subjected to next‐generation sequencing (NGS). Results Significantly higher rates of valid results (95.1% vs. 78%, respectively, p = 0.04) and EGFR common mutation detection (94.3% vs. 77.1%, respectively, p = 0.047) were obtained through ddPCR than through the cobas EGFR Mutation Test. The sensitivities of ddPCR and cobas were 94.3% and 75.6%, respectively. The concordance rate for EGFR mutation detection through ddPCR and the cobas EGFR Mutation Test was 75.6% and that for EGFR mutation detection in CSF and plasma ctDNA was 28.1%. In osimertinib‐resistant CSF samples, all original EGFR mutations were detected through NGS. MET amplification and CCDC6‐RET fusion were demonstrated in one patient each (9.1%). Conclusions The cobas EGFR Mutation Test, ddPCR, and NGS appear to be feasible methods for analyzing CSF ctDNA in patients with NSCLC and LM. In addition, NGS may provide comprehensive information regarding the mechanisms underlying osimertinib resistance.
- Published
- 2023
- Full Text
- View/download PDF
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