1. The interaction between serum amyloid A and Toll‐like receptor 2 pathway regulates inflammatory cytokine secretion in human gingival fibroblasts
- Author
-
Changyi Li, Wen Lai, Jiashan Li, Shao-Yun Jiang, Yu-Zhu Mu, and Liting Song
- Subjects
0301 basic medicine ,animal diseases ,Gingiva ,Inflammation ,Proinflammatory cytokine ,03 medical and health sciences ,0302 clinical medicine ,hemic and lymphatic diseases ,medicine ,Humans ,Serum amyloid A ,Cells, Cultured ,Periodontitis ,Serum Amyloid A Protein ,Toll-like receptor ,business.industry ,Interleukin ,030206 dentistry ,Fibroblasts ,medicine.disease ,Toll-Like Receptor 2 ,stomatognathic diseases ,TLR2 ,030104 developmental biology ,Immunology ,Cytokines ,Periodontics ,Cytokine secretion ,medicine.symptom ,business - Abstract
BACKGROUND Serum amyloid A (SAA) has been identified to trigger inflammation response, and play a crucial role in chronic inflammatory diseases. However, the regulatory mechanism of SAA still remains unclear during the development of periodontitis METHODS: SAA mRNA and protein expression were detected in healthy and inflammatory gingival tissues using real-time polymerase chain reaction (PCR) and immunohistochemistry. Human recombinant SAA (Apo-SAA), Pam3CSK4 (a Toll-like receptor (TLR) 2 ligand), siRNA-SAA, or TLR2 neutralizing antibody was applied to treat human gingival fibroblasts, respectively, or combined. SAA, TLRs, and inflammatory cytokines interleukin (IL)-6 and IL-8 were analyzed by real-time PCR, western blotting, or enzyme-linked immunosorbent assay. RESULTS SAA expression increased in human inflammatory gingival tissues from patients with periodontitis (P
- Published
- 2019