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Your search keyword '"Yamauchi, Nobuhiko"' showing total 3 results
Start Over Author "Yamauchi, Nobuhiko" Topic bovine Publisher wiley-blackwell
3 results on '"Yamauchi, Nobuhiko"'

1. Conserved Roles of Fibroblast Growth Factor Receptor 2 Signaling in the Regulation of Inner Cell Mass Development in Bovine Blastocysts

Author

Akizawa, Hiroki, Nagatomo, Hiroaki, Odagiri, Haruka, Kohri, Nanami, Yamauchi, Nobuhiko, Yanagawa, Yojiro, Nagano, Masashi, Takahashi, Masashi, and Kawahara, Manabu

Subjects
blastocyst, bovine, inner cell mass, cell-lineage specification, fibroblast growth factor signaling
Abstract

A common process during preimplantation mammalian development is blastocyst formation, which utilizes signaling through fibroblast growth factor receptor 2 (FGFR2), yet the mechanisms through which FGFR2 signaling affect preimplantation development in bovine embryos remain incompletely understood. Here, we used RNA-interference to investigate the in vitro development, the frequency of blastomere apoptosis, and the mRNA expression of developmental marker genes in FGF receptor 2-knockdown (FGFR2-KD) bovine embryos. A reduction in FGFR2 mRNA did not affect preimplantation development or the frequency of apoptotic blastomeres, but did enhanced proliferation of the inner cell mass in blastocysts (P< 0.05) -which differs from the phenotype reported for bovine embryos using a pharmacological approach (treatment with the pan-FGFR blocker PD173074), but agrees with previous results obtained using mouse embryos. Moreover, the expression of an epiblast marker gene, NANOG, and a primitive endoderm marker gene, GATA6, remained unchanged, whereas the expression of another primitive endoderm marker gene, HNF4A, was significantly reduced in FGFR2-KD embryos. Therefore, FGFR2 signaling appears to be associated with the regulation of inner cell mass development and proliferation during blastocyst formation in cattle.

Published
2016

2. Analysis of differentially expressed genes and the promoters in bovine endometrium throughout estrus cycle and early pregnancy.

Author

Musavi, Sayed A.A., Yamashita, Seiya, Fujihara, Taisuke, Masaka, Hironori, Islam, Md. Rashedul, Kim, Sangwan, Gotoh, Takafumi, Kawahara, Manabu, Tashiro, Kosuke, and Yamauchi, Nobuhiko

Subjects
GENE expression, ESTRUS, SEXUAL cycle, PREGNANCY in animals, ENDOMETRIUM, TRANSCRIPTION factors, CATTLE
Abstract

Endometrial gene expression is primarily regulated by the ovarian steroids and pregnancy recognition factors. This study was aimed to characterize differential expression genes (DEGs) in bovine endometrium together with the analysis of their promoter region. Bovine uteri at follicular stage (FS), luteal stage (LS), and implantation stage (IS) at Day 18 of pregnancy were collected. Total RNA extracted and prepared cDNA were then subjected to high‐throughput sequencing. For promoter analysis, 1 kb upstream promoter region of each DEG was analyzed. The numbers of highly expressed DEGs were 496 and 597 at FS and LS, respectively. When compared the gene expression of IS with LS, 383 and 346 DEGs showed higher and lower expression at IS, respectively. It was also observed that 20–30 transcription factors (TFs) were included in each DEGs. In addition, promoter analyses estimated 150–160 TFs for each stage. DLX4 and interferon regulatory factor 4 (IRF4) at FS, and IRF5, IRF9, STAT1, and STAT2 at IS were in common to DEGs and estimated TFs, respectively. This study highlighted potential molecular mechanisms controlling endometrial function during estrus cycle and IS, which will further guide to better understand the endometrial functions in future studies. [ABSTRACT FROM AUTHOR]

Published
2018
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3. Development of an in vitro model for the analysis of bovine endometrium using simple techniques.

Author

Yamauchi, Keisuke, Yamauchi, Nobuhiko, Yamagami, Kazuki, Nakamura, Nobuhisa, Yamashita, Seiya, Islam, Md. Rashedul, Tabata, Shoji, Yahiro, Kanji, Tamura, Tomoko, Hashizume, Kazuyoshi, and Hattori, Masa‐aki

Subjects
*ENDOMETRIUM, *IMMUNOFLUORESCENCE, *METALLOPROTEINASES, *IN vitro studies, *CONTROL groups, *HEPATOCYTE growth factor, *INTERFERON receptors, *HORMONE receptors
Abstract

This study aimed to develop an in vitro model for the analysis of the bovine endometrium. Immunofluorescent staining revealed that the hetero-spheroids and the cultured explants showed almost similar structure in the localization of bovine endometrial epithelial cells and endometrial stromal cells, except the glandular-like structure of the epithelial cells inside the explants. Gelatin zymography revealed that the hetero-spheroids did not express matrix metalloproteinases ( MMPs) after 4 days of culture, but strong MMP expressions were observed in the cultured explants until 7 days of culture. Additionally, expression of progesterone receptor ( PR), estrogen receptor alpha ( ERα), type I interferon receptor 1 (IFNAR1) and 2 (IFNAR2) messenger RNA was observed both in the homo- and hetero-spheroids. The expression of oxytocin receptor ( OTR) mRNA in E2 and E2+ P4 (1,3,5(10)-Estratrien-3, 17β-diol + 4-Pregnen-3, 20-dinone) treated groups were significantly ( P < 0.05) higher than that of the control group of spheroids. In case of cultured explants, the expression of PR and OTR mRNA were significantly ( P < 0.05) higher in E2 treated groups compared to the control groups. Hepatocyte growth factor ( HGF) mRNA expression was also higher in P4 treated groups at 10 days in culture ( P < 0.05). In a nutshell, the in vitro model developed in this study for the analysis of the endometrium may provide a new platform for extensive research on bovine endometrial function. [ABSTRACT FROM AUTHOR]

Published
2015
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