1. Induction of epithelial-mesenchymal transition with O-glycosylated oncofetal fibronectin
- Author
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Ding, Yao, Gelfenbeyn, Kirill, Freire-de-Lima, Leonardo, Handa, Kazuko, and Hakomori, Sen-itiroh
- Subjects
EPITHELIAL cells ,EMBRYOLOGY ,CANCER invasiveness ,FIBRONECTINS ,LUNG cancer ,TRANSFORMING growth factors ,CANCER cells - Abstract
Abstract: Epithelial-mesenchymal transition (EMT) has been shown to play a key role in embryogenesis and cancer progression. We previously found that fibronectin (FN) carrying O-GalNAc at a specific site is selectively expressed in cancer and fetal cells/tissues, and termed oncofetal FN (onfFN). Here, we show that (i) a newly-established monoclonal antibody against FN lacking the O-GalNAc, termed normalFN (norFN), is useful for isolation of onfFN, (ii) onfFN, but not norFN, can induce EMT in human lung carcinoma cells, (iii) onfFN has a synergistic effect with transforming growth factor (TGF)β1 in EMT induction. [Copyright &y& Elsevier]
- Published
- 2012
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