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The article discusses Mitochondrial Neurogastrointestinal Encephalomyopathy (MNGIE), a rare autosomal recessive disorder caused by mutations in the thymidine phosphorylase (TP) gene. Patients with MNGIE experience a range of symptoms affecting the gastrointestinal (GI) tract and the nervous system, including chronic intestinal pseudo-obstruction (CIPO). Diagnosis of MNGIE is often delayed due to non-specific GI symptoms, leading to misdiagnoses with conditions like Crohn's disease. Genetic testing is crucial for confirming MNGIE, and treatment options include nutrition support, stem cell transplantation, and liver transplantation. The article highlights the importance of early diagnosis and targeted treatment to improve patient outcomes. [Extracted from the article]

Glycerol kinase (GK) participates in triglyceride (TG) synthesis by catalyzing glycerol metabolism. Whether GK contributes to nonalcoholic fatty liver (NAFL) is unclear. The expression of hepatic Gk is found to be increased in diet‐induced and genetic mouse models of NAFL and is positively associated with hepatic SREBP‐1c expression and TG levels. Cholesterol and fatty acids stimulate GK expression in hepatocytes. In HFD‐induced NAFL mice, knockdown of hepatic Gk decreases expression of SREBP‐1c and its target lipogenic genes as well as DGAT1/2, increases serum glycerol levels, decreases serum TG levels, and attenuates hepatic TG accumulation. Overexpression of GK in hepatocytes in mice or in culture produces opposite results. Mechanistic studies reveal that GK stimulates SREBP‐1c transcription directly by binding to its gene promoter and indirectly by binding to SREBP‐1c protein, thereby increasing lipogenic gene expression and de novo lipogenesis. Studies with truncated GK and mutant GKs indicate that GK induces SREBP‐1c transcription independently of its enzyme activity. GK contributes to lipid homeostasis under physiological conditions by catalyzing glycerol metabolism rather than by regulating SREBP‐1c transcription. Collectively, these results demonstrate that increased hepatic GK promotes de novo lipogenesis and TG synthesis in NAFL by stimulating SREBP‐1c transcription and DGAT1/2 expression and catalyzing glycerol metabolism. [ABSTRACT FROM AUTHOR]

Acylcarnitine (ACar) is a novel fuel source for activating thermogenesis in brown adipose tissue (BAT). However, whether ACar metabolism underlies BAT thermogenesis decline with aging remain unclear. Here, the L‐carnitine‐treated young and aging mice were used to investigate the effects of activation of ACar metabolism on BAT thermogenesis during aging. We showed that long term L‐carnitine feeding, which results in an elevation in circulating ACar levels, failed to improve cold sensitivity of aging mice, which still displayed impaired thermogenesis and ACar metabolism in interscapular BAT (iBAT). The RNA‐sequencing was used to identify the key regulator for the response of aging mice to LCar induced activation of ACar metabolism in BAT, and we identified Sirt3 as a key regulator for the response of aging mice to L‐carnitine induced activation of ACar metabolism in iBAT. Then the adipose‐specific Sirt3 knockout (Sirt3 AKO) mice were used to investigate the role of Sirt3 in ACar metabolism and thermogenesis of BAT and explore the underlying mechanism, and the results showed that Sirt3 AKO mice displayed defective ACar metabolism and thermogenesis in iBAT. Mechanically, Sirt3 regulated ACar metabolism via HIF1α‐PPARα signaling pathway to promote iBAT thermogenesis, and knockdown or inhibition of HIF1α ameliorated impaired ACar metabolism and thermogenesis of iBAT in the absence of Sirt3. Collectively, we propose that Sirt3 regulated ACar metabolism is critical in maintaining thermogenesis in BAT of aging mice, which can promote the development of anti‐aging intervention strategy. [ABSTRACT FROM AUTHOR]

The design of multicomponent materials has captured considerable attention due to its extraordinary ability to tailor functional properties. However, how a single element affects the behavior of the overall material has yet to be explored in depth. In this study, the heteroepitaxy of high entropy (Fe, Co, Ni, Cr, Mn)3O4 films with varying strain states are investigated in magnetic performance. It is discovered that the high entropy oxide thin film with compressive strain exhibits an effect of crystalline magnetic anisotropy. Diverse analyses provide a detailed understanding of high entropy magnetic oxide systems, including X‐ray diffraction, reciprocal space mapping, macroscopic magnetic characterization, X‐ray absorption spectroscopy (XAS), etc. Notably, the element‐specific XAS technique proves effective in uncovering the origin of the crystalline magnetic anisotropy. Due to the substrate‐induced epitaxial strain, the eg orbitals of Mn3+ form different energy levels, leading to different preferred electron occupancy. The exploration of magnetic properties in epitaxial high entropy oxide film is then raveled. By navigating the complexities introduced by the random atom distribution and intricate magnetic interactions, this study pioneers novel methodologies for probing the core physics of high entropy oxides. [ABSTRACT FROM AUTHOR]

Soils are a major source of global nitric oxide (NO) emissions. However, estimates of soil NO emissions have large uncertainties due to limited observations and multifactorial impacts. Here, we mapped global soil NO emissions, integrating 1356 in‐situ NO observations from globally distributed sites with high‐resolution climate, soil, and management practice data. We then calculated global and national total NO budgets and revealed the contributions of cropland, grassland, and forest to global soil NO emissions at the national level. The results showed that soil NO emissions were explained mainly by N input, water input and soil pH. Total above‐soil NO emissions of the three vegetation cover types were 9.4 Tg N year−1 in 2014, including 5.9 Tg N year−1 (1.04, 95% confidence interval [95% CI]: 0.09–1.99 kg N ha−1 year−1) emitted from forest, 1.7 Tg N year−1 (0.68, 95% CI: 0.10–1.26 kg N ha−1 year−1) from grassland, and 1.8 Tg N year−1 (0.98, 95% CI: 0.42–1.53 kg N ha−1 year−1) from cropland. Soil NO emissions in approximately 57% of 213 countries surveyed were dominated by forests. Our results provide updated inventories of global and national soil NO emissions based on robust data‐driven models. These estimates are critical to guiding the mitigation of soil NO emissions and can be used in combination with biogeochemical models. [ABSTRACT FROM AUTHOR]

Perovskite ferroelectric thin films exhibit unique dielectric and piezoelectric properties owing to their internal polarized domains that accommodate the out‐of‐plane (ferroelectric) and in‐plane (ferroelastic) polarization‐induced electrostatic and elastic energy. These domains are generally treated as 2D defects with distinctive differences in domain morphology and domain‐wall characteristics, although they are indeed 3D volumetric defects. Here, by using atomistic simulation and microscopy characterization, a "pseudo‐ferroelectric domain" that has the morphology similar to a ferroelectric domain but holds the same defect character of ferroelastic domain‐wall, i.e., semi‐coherent (100)matrix||(100)domain interface is identified. Such pseudo‐ferroelectric domain walls will play a critical role in the migration kinetics of ferroelastic domains and in the piezoelectric responses of ferroelectric thin films during cyclic mechanical/electrical loading. The study throws light on a novel aspect of domains, namely, the 3D configuration and mobility of domain walls, and their role in the overall domain engineering. [ABSTRACT FROM AUTHOR]

Background: Simian immunodeficiency virus (SIV) infection in rhesus macaques (Macaca mulatta) can lead to the development of SIV encephalitis (SIVE), which is closely related to human immunodeficiency virus (HIV)‐induced dementia. Methods: This was done by analyzing SIV and SIVE encephalitis in infected M. mulatta hippocampus samples from two microarray data sets, identifying two groups of common differentially expressed genes and predicting associated protein interactions. Results: We found that eight genes—MX1, B2M, IFIT1, TYMP, STAT1, IFI44, ISG15, and IFI27—affected the negative regulation of biological processes, hepatitis C and Epstein–Barr viral infection, and the toll‐like receptor signaling pathway, which mediate the development of encephalitis after SIV infection. In particular, STAT1 played a central role in the process by regulating biopathological changes during the development of SIVE. Conclusion: These findings provide a new theoretical basis for the treatment of encephalopathy after HIV infection by targeting STAT1. [ABSTRACT FROM AUTHOR]

Nowadays, the use of topographical features and electrical conductivity of scaffolds at the cell-substrate interface for effectively regulating cell growth and differentiation have gained increasing attention due to great demands for tissue engineering. Herein, a facile approach to the growth of highly disordered graphene nanosheets (HDGNs) is demonstrated on a cheap and weaving quartzbraided structure as a functionalized scaffold for the differentiation of nerve cells. The patterned aligned structure can effectively integrate the advantages of a conductive graphene-functional interface (favorable for cell attachment and growth), topologically woven surface structure, providing a flexible and multifunctional regulatory platform for nerve cell growth. Compared with monocrystal polycrystalline graphene, amorphous graphene has high biocompatibility due to sufficient active sites, and has high conductivity to the composite nonconductive substrate, which can realize electrical stimulation (ES) of cell differentiation. Herein, the HDGN/quartz fabric with high biocompatibility (the cell viability is 98%), and great electrical conductivity, is proved. Then, the applied ES coupled with HDGN/quartz fabric significantly enhances selective neuronal differentiation into neurons (the differentiation growth rate is 131%). Collectively, herein, a new material basis is provided for electric induction of cell growth and differentiation, providing more possibilities for the development of intelligent biological applications. [ABSTRACT FROM AUTHOR]

Photoactive Cu(I) complexes are promising luminescent materials owing to their abundant reserves and marvelous biocompatibility. In this work, we report the synthesis and characterization of two binuclear sulfur‐bridged Cu(I) complexes (POP)2Cu2(μ‐HS/Cl)2 ⋅ 0.92MeOH 1 (POP=(oxybis(2,1‐phenylene))bis(diphenylphosphine)) and (POP)2Cu2(μ‐HS)22, which were prepared by one‐pot facile solvothermal method with copper‐4‐tert‐butylbenzenethiol (CuSC6H4tBu) and POP as precursors. Complex 1 exhibits sky‐blue emission while complex 2 displays blue. Complex 1 was systemically investigated by PXRD, UV‐vis spectrum, IR, TGA and steady‐state spectra. Temperature‐dependent steady‐state emission and decay lifetime indicate that complex 1 is a typical phosphorescent emitter. [ABSTRACT FROM AUTHOR]

Magnetic spinel oxides have attracted extensive research interest due to their rich physics and wide range of applications. However, these materials invariably suffer suppressed magnetization, due to structural imperfections (e.g., disorder, anti‐site defects, etc.). Herein, a dramatic enhanced magnetization is obtained with an increasement of 5 µB/u.c in CoFe2O4 (CFO) through ionic liquid gating induced hydrogen doping. The intercalated hydrogen ions lead to both distinct lattice expansion of ≈0.7% and notable Fe valence state reduction through electron doping, in which ≈17% Fe3+ is reduced into Fe2+. These facts collectively trigger a site‐specific spin‐flip on tetrahedrally coordinated Co2+ sites that enhances the net ferrimagnetic moment nearly to its theoretical maximum for perfect CFO. [ABSTRACT FROM AUTHOR]

Objective: Lenvatinib plus anti‐programmed death‐1 (anti‐PD‐1) antibody combinations have shown potent anti‐tumor effect in phase I/II trials in advanced or unresectable hepatocellular carcinoma (HCC), but real‐world data are limited. Methods: To investigate the effectiveness and safety of lenvatinib plus anti‐PD‐1 antibodies in a real‐world cohort, we retrospectively evaluated 210 patients with unresectable or advanced HCC treated with these regimens between October 2018 and February 2022. Results: The objective response rate and disease control rate per Response Evaluation Criteria in Solid Tumors (RECIST) v1.1 were 28.1% and 75.2%. Median overall survival (OS) and progression‐free survival (PFS) in the overall cohort were 17.2 and 8.4 months, respectively. Median OS and PFS of patients receiving first‐line treatment reached 18.9 and 9.6 months. Median OS was significantly longer in patients with Child‐Pugh class A versus B (18.8 vs. 5.9 months, respectively), as was median PFS (9.1 vs. 4.4 months). Patients with albumin–bilirubin (ALBI) grade 1 versus grade 2/3 also had significantly greater median OS (23.5 vs. 13.4 months). Treatment‐related adverse events (AEs) occurred in 79.5% of patients. Patients with ALBI grade 2/3 had a higher rate of grade 3/4 AEs than patients with ALBI grade 1 (57.5% vs. 38.5%). Conclusion: Lenvatinib combined with anti‐PD‐1 antibody therapy was effective in patients with sufficient liver function reserve. Further study is needed to improve therapeutic efficacy and AE management in patients with Child‐Pugh class B or ALBI grade 2/3. [ABSTRACT FROM AUTHOR]

The polysaccharide chitin is a major scaffolding molecule in the insect cuticle. In order to be functional, both chitin amounts and chitin organization have been shown to be important parameters. Despite great advances in the past decade, the molecular mechanisms of chitin synthesis and organization are not fully understood. Here, we have characterized the function of the Chitinase 6 (Cht6) in the formation of the wing, which is a simple flat cuticle organ, in the fruit fly Drosophila melanogaster. Reduction of Cht6 function by RNA interference during wing development does not affect chitin organization, but entails a thinner cuticle suggesting reduced chitin amounts. This phenotype is opposed to the one reported recently to be caused by reduction of Cht10 expression. Probably as a consequence, cuticle permeability to xenobiotics is enhanced in Cht6‐less wings. We also observed massive deformation of these wings. In addition, the shape of the abdomen is markedly changed upon abdominal suppression of Cht6. Finally, we found that suppression of Cht6 transcript levels influences the expression of genes coding for enzymes of the chitin biosynthesis pathway. This finding indicates that wing epidermal cells respond to activity changes of Cht6 probably trying to adjust chitin amounts. Together, in a working model, we propose that Cht6‐introduced modifications of chitin are needed for chitin synthesis to proceed correctly. Cuticle thickness, according to our hypothesis, is in turn required for correct organ or body part shape. The molecular mechanisms of this processes shall be characterized in the future. [ABSTRACT FROM AUTHOR]

Laser processing of pure Cu is often challenging because of Cu's very low absorptivity to most laser beams, and this difficulty is applicable to the recently developed laser powder bed fusion (LPBF) additive manufacturing (AM). How to realize high‐quality LPBF forming is urgently pursued to maximize its advanced manufacturing potential in directly making various Cu parts. Herein, two approaches have been applied, individually or simultaneously, to address the poor printability problem of the pure Cu: one is to conduct intentional oxidation treatment to the pure Cu powder to vary its surface structure and then enhance its laser absorptivity; and the other is to adopt laser remelting to counter its high thermal conductivity and provide extra liquid phase for better densification. Various research means were used to investigate mechanical performances, microstructural details, and electrical performance of the as‐printed Cu. Results show that a maximum relative density of ≈99.0% can be realized by using the two approaches simultaneously, while oxidation temperature and remelting times are the two important factors to be considered. The methodology developed in this study is expected to be applicable to other highly reflective materials as well. [ABSTRACT FROM AUTHOR]

Background: The tumor‐stromal ratio (TSR) has been verified to be a prognostic factor in many solid tumors. In most studies, it was manually assessed on routinely stained H&E slides. This study aimed to assess the TSR using image analysis algorithms developed by the Qupath software, and integrate the TSR into a nomogram for prediction of the survival in invasive breast cancer (BC) patients. Methods: A modified TSR assessment algorithm based on the recognition of tumor and stroma tissues was developed using the Qupath software. The TSR of 234 invasive BC specimens in H&E‐stained tissue microarrays (TMAs) were assessed with the algorithm and categorized as stroma‐low or stroma‐high. The consistency of TSR estimation between Qupath prediction and pathologist annotation was analyzed. Univariable and multivariable analyses were applied to select potential risk factors and a nomogram for predicting survival in invasive BC patients was constructed and validated. An extra TMA containing 110 specimens was obtained to validate the conclusion as an independent cohort. Results: In the discovery cohort, stroma‐low and stroma‐high were identified in 43.6% and 56.4% cases, respectively. Good concordance was observed between the pathologist annotated and Qupath predicted TSR. The Kaplan–Meier curve showed that stroma‐high patients were associated with worse 5‐DFS compared to stroma‐low patients (p = 0.007). Multivariable analysis identified age, T stage, N status, histological grade, ER status, HER‐2 gene, and TSR as potential risk predictors, which were included in the nomogram. The nomogram was well calibrated and showed a favorable predictive value for the recurrence of BC. Kaplan–Meier curves showed that the nomogram had a better risk stratification capability than the TNM staging system. In the external validation of the nomogram, the results were further validated. Conclusions: Based on H&E‐stained TMAs, this study successfully developed image analysis algorithms for TSR assessment and constructed a nomogram for predicting survival in invasive BC. [ABSTRACT FROM AUTHOR]

In condensed matter physics, oxide materials show various intriguing physical properties. Therefore, many efforts are made in this field to develop functional oxides. Due to the excellent potential for tin‐based perovskite oxides, an expansion of new related functional compounds is crucial. This work uses a heteroepitaxial approach supported by theoretical calculation to stabilize PbSnO3 thin films with different orientations. The analyses of X‐ray diffraction and transmission electron microscopy unveil the structural information. A typical antiferroelectric feature with double hysteresis and butterfly loops is observed through electrical characterizations consistent with the theoretical prediction. The phase transition is monitored, and the transition temperatures are determined based on temperature‐dependent structural and electrical characterizations. Furthermore, the microscopic antiferroelectric order is noticed under atomic resolution images via scanning transmission electron microscopy. This work offers a breakthrough in synthesizing epitaxial PbSnO3 thin films and comprehensively understanding its anisotropic antiferroelectric behavior. [ABSTRACT FROM AUTHOR]

What is already known and objective: Sedation is routinely provided for patients undergoing gastrointestinal endoscopy. Remimazolam tosilate is a novel and short‐acting sedative agent that has been used for sedation during endoscopic procedures. The optimal dose of remimazolam in gastrointestinal endoscopy for patients with liver cirrhosis has not been elucidated. Background: To determine the effective dose of remimazolam tosilate with adjuvant sufentanil for sedation in patients with liver cirrhosis undergoing oesophagogastric varices screening endoscopy. Material and Methods: Patients aged 18–65 years with liver cirrhosis undergoing screening endoscopy for oesophagogastric varices were recruited. Sufentanil 0.15 μg/kg was given intravenously at 2 min before administration of remimazolam tosilate. The initial dose of remimazolam was 0.1 mg/kg and adjusted by 0.025 mg/kg as a step size, based on the Dixon and Massay up‐and‐down sequential method. Inclusion of patients was stopped after eight crossovers and the calculated median effective dose (ED50) of remimazolam for successful endoscopy was obtained by calculating the mean of midpoint of all crossovers. Furthermore, a probit regression was applied to establish the dose–response curve of remimazolam and further assess the 95% effective dose (ED95) of remimazolam. Results: The calculated ED50 of remimazolam for successful endoscopy using the mean of midpoint of all crossovers was 0.097 mg/kg (95% CI, 0.004–0.099 mg/kg). Using the probit regression analysis, the ED50 and ED95 of remimazolam for successful endoscopy was 0.097 mg/kg (95% CI, 0.004–0.099 mg/kg) and 0.107 mg/kg (95% CI, 0.103–0.336 mg/kg), respectively. No adverse events were observed throughout the study period. Conclusions: This pilot study suggests that the ED50 and ED95 of remimazolam tosilate with adjuvant sufentanil for sedation in liver cirrhosis patients undergoing oesophagogastric varices screening endoscopy was 0.097 and 0.107 mg/kg, respectively. [ABSTRACT FROM AUTHOR]

Comprehensive, spatially explicit understanding of N loss from N input in China's maize (Zea mays L.) belt is needed to improve N management and develop N loss mitigation measures for specific locations. However, accurate estimation of N loss remains uncertain mainly because of spatial heterogeneity of emission factors (EFs) and activity data. Here, we built random‐forest models to predict EFs for each N loss pathway (dinitrogen, nitric oxide, nitrous oxide and ammonia emissions, nitrate leaching, and N runoff) considering soil and climate factors. We then used location‐ and crop‐specific activity data to understand N loss and surplus on a 1‐ × 1‐km grid. Results showed that the N surplus and total N loss averaged 135.4 (95% confidence interval [CI], 36.6–261.5) and 87.6 (95% CI, 32.9–162.5) kg N ha–1, respectively, and nitrate leaching and ammonia emissions were primary loss pathways. There was a significant correlation between N surplus and total N loss in spatial distribution, and regions with high N surplus and total N loss were mainly in the northern China Plain and Sichuan Basin, while regions with low surplus and loss could be found in Heilongjiang and Jilin provinces. Our results are beneficial to understand specific crop N budget and develop location‐specific N management strategies. Core Ideas: A spatially explicit assessment of N loss from N input was mapped in China's maize belt.The N surplus and total N loss averaged 135.4 and 87.6 kg N ha‐1, respectively.High N surplus and total N loss was recorded in the northern China Plain and Sichuan Basin. [ABSTRACT FROM AUTHOR]

Objective: To investigate the correlation between melatonin and osteoarthritis (OA) in rats. To explore the relevant mechanisms in the occurrence and development of osteoarthritis in rats, and to further understand the disease of osteoarthritis. Methods: Forty healthy 6‐month‐old male SD rats were randomly divided into two groups: sham and drug intervention groups. Pre‐OA modeling, enzyme‐linked immunosorbent assay was employed to detect the levels of IL‐1β, IL‐6, COX‐2, and melatonin in the serum of the rats in each group. For OA modeling, we administered an injection of papain into the knee cavity of all rats. The levels of IL‐1β, IL‐6, and COX‐2 in the serum of rats in each group were detected 2 weeks after the modeling. Additionally, 2 weeks after the modeling, the rats in the drug intervention group were intraperitoneally injected with melatonin antagonists. The rats in the sham group were intraperitoneally injected with normal saline for 2 weeks. The levels of IL‐1β, IL‐6, and COX‐2 in the serum of each group were measured at the second, third, and fourth weeks after the drug intervention, and the levels of melatonin in the serum were measured at the second week after the drug intervention. Finally, the rats were euthanized by cervical dislocation, and pathological sections were collected from the knee joint to observe the pathological tissue changes under a microscope, and Mankin score was determined. The independent samples t‐test method was used for analysis. Results: The imaging examination after the drug intervention showed that the modeling of knee osteoarthritis in rats was successful. In the pathological findings, HE staining showed a legible cartilage structure of each layer, with cartilage proliferation and partial cartilage tearing to the radial layer. The tide line was intact; toluidine blue staining revealed more obvious changes. The differences among the mean values of IL‐6, IL‐1β, and COX‐2 measured in each period were statistically significant (t = 5.50, p < 0.05). The measured mean values of IL‐6, IL‐1β, and COX‐2 revealed statistically significant differences among the groups (t = 2.01, p < 0.05). The intergroup comparison of the Mankin scores in each period showed statistically significant differences. Conclusion: Melatonin may inhibit inflammation and associated oxidative stress on the surface of knee cartilage. It may be related to the repair and regeneration of articular surface cartilage during the development of OA in the rat knee joint. [ABSTRACT FROM AUTHOR]

Nicotinamide adenine dinucleotide phosphate (NADPH)‐cytochrome P450 reductase (CPR) is involved in the metabolism of endogenous and exogenous substances, and detoxification of insecticides. RNA interference (RNAi) of CPR in certain insects causes developmental defects and enhanced susceptibility to insecticides. However, the CPR of Acyrthosiphon pisum has not been characterized, and its function is still not understood. In this study, we investigated the biochemical functions of A. pisum CPR (ApCPR). ApCPR was found to be transcribed in all developmental stages and was abundant in the embryo stage, and in the gut, head, and abdominal cuticle. After optimizing the dose and silencing duration of RNAi for downregulating ApCPR, we found that ApCPR suppression resulted in a significant decrease in the production of cuticular and internal hydrocarbon contents, and of cuticular waxy coatings. Deficiency in cuticular hydrocarbons (CHCs) decreased the survival rate of A. pisum under desiccation stress and increased its susceptibility to contact insecticides. Moreover, desiccation stress induced a significant increase in ApCPR mRNA levels. We further confirmed that ApCPR participates in CHC production. These results indicate that ApCPR modulates CHC production, desiccation tolerance, and insecticide susceptibility in A. pisum, and presents a novel target for pest control. [ABSTRACT FROM AUTHOR]

Orthogonal least squares (OLS) algorithm, as a popular greedy algorithm for sparse signal recovery, has attracted much attention in recent years. In this paper, the completely perturbed model is considered, y∼=Ax+b,A∼=A+E, $\tilde{\mathbf{y}}=\mathbf{A}\mathbf{x}+\mathbf{b},\tilde{\mathbf{A}}=\mathbf{A}+\mathbf{E},$ where x is K‐sparse. Assume that the matrix A∼ $\tilde{\mathbf{A}}$ has unit ℓ2‐norm columns and satisfies the restricted isometry property of order K + 1. Given stopping criterion ‖r∼k‖≤ε0 ${\Vert}{\tilde{\mathbf{r}}}^{k}{\Vert}\le {\varepsilon }_{0}$, then the authors prove that the OLS algorithm precisely recovers the support of the K‐sparse signal x in at most K iterations under the suitable constraint condition on x. In addition, the authors also show that the authors' conclusion is superior to the current results. [ABSTRACT FROM AUTHOR]

Our knowledge of the coordination of intergenerational inheritance and offspring metabolic reprogramming by gastrointestinal endocrine factors is largely unknown. Here, we showed that secretin (SCT), a brain‐gut peptide, is downregulated by overnutrition in pregnant mice and women. More importantly, genetic loss of SCT in the maternal gut results in undesirable phenotypes developed in offspring including enhanced high‐fat diet (HFD)‐induced obesity and attenuated browning of inguinal white adipose tissue (iWAT). Mechanistically, loss of maternal SCT represses iWAT browning in offspring by a global change in genome methylation pattern through upregulation of DNMT1. SCT functions to facilitate ubiquitination and degradation of DNMT1 by activating AMPKα, which contributes to the observed alteration of DNMT1 in progeny. Lastly, we showed that SCT treatment during pregnancy can reduce the development of obesity and improve glucose tolerance and insulin resistance in offspring of HFD‐fed females, suggesting that SCT may serve as a novel biomarker or a strategy for preventing metabolic diseases. Synopsis: Maternal secretin promotes white adipose tissue browning in the offspring by inducing a global change in genome methylation pattern through the DNA methyltransferase DNMT1. Genetic loss of secretin in the maternal gut results in disrupted glucose and lipid homeostasis in the offspring.Maternal secretin promotes white adipose tissue browning in the offspring.Depletion of maternal SCT leads to an AMPKAα dependent increase in DNMT1 protein levels in the white adipose tissue of the offspring.Maternal secretin reprograms white adipose tissue methylation of the offspring. [ABSTRACT FROM AUTHOR]

A growing body of evidence showed that environmental enrichment (EE) ameliorated footshock-induced fear behavior of posttraumatic stress disorder (PTSD). However, no research comprehensively tested the effect of EE, cue, and the combination of EE and cue in footshock-induced fear behavior of PTSD symptoms. The present study addressed this issue and examined whether the medial prefrontal cortex (mPFC, including the cingulate cortex 1 (Cg1), prelimbic cortex (PrL), and infralimbic cortex (IL)), the nucleus accumbens (NAc), the basolateral amygdala (BLA), and the hippocampus (e.g., CA1, CA3, and dentate gyrus (DG)) regulated the amelioration of the EE, cue, or the combination of EE and cue. The results showed that EE or cue could reduce fear behavior. The combination of EE and cue revealed a stronger decrease in fear behavior. The cue stimulus may play an occasion setting or a conditioned stimulus to modulate the reduction in fear behavior induced by footshock. Regarding the reduction of the EE in fear behavior, the Cg1 and IL of the mPFC and the NAc upregulated the c-Fos expression; however, the BLA downregulated the c-Fos expression. The mPFC (i.e., the Cg1, PrL, and IL) and the hippocampus (i.e., the CA1, CA3, and DG) downregulated the c-Fos expression in the suppression of the cue in fear behavior. The interaction of EE and cue in reduction of fear behavior occurred in the Cg1 and NAc for the c-Fos expression. The data of c-Fos mRNA were similar to the findings of the c-Fos protein expression. These findings related to the EE and cue modulations in fear behavior may develop a novel nonpharmacological treatment in PTSD. [ABSTRACT FROM AUTHOR]

In order to research the force state of the piers subjected to a ship collision under the protection of floating anti-collision facilities, this study uses nonlinear spring connections to simulate the impact of ship damping and incidental water quality in the collision area. BIM technology is used to realize a safety evaluation method for the anti-collision floating box protection when the ship is colliding with piers. Established a BIM-based parametric preprocessing model for ships, piers, and anti-collision facilities, and opened the interface with ABAQUS longitudinally. After realizing the parameter adjustment of the BIM model, the visual parameter adjustment can be realized without destroying the boundary conditions, load conditions, and meshing. Taking a rigid frame bridge as an example, the most disadvantage position of the bridge pier under ship collision is determined by the parameterization method. At the same time, multi-condition analysis was carried out on the ship impacting the pier anti-collision floating box at different angles, different tonnages, and different speeds. Finally, the analysis results are traced back to the BIM model, achieving the unified integration of BIM model information and finite element analysis results and the purpose of visual analysis of any working conditions. The results show that the use of BIM parameterization technology to achieve linkage with the finite element preprocessing model can improve the efficiency of multi-condition sensitivity analysis and achieve the purpose of visual dynamic adjustment. The safety assessment analysis of the pier under the protection of the anti-collision pontoon on the pier under various working conditions shows that the anti-collision pontoon effectively reduces the hazard of the ship colliding with the pier, and the impact force gradually increases with the change from the oblique collision to the frontal collision. The peak impact force increases with the weight of the ship and shows a nonlinear relationship, such that the peak value of impact force increases with the speed increase, and the speed and the peak values of impact force show basically a linear relationship. [ABSTRACT FROM AUTHOR]

Objective: To better understand the risks of bisphosphonates in order to develop guidance for appropriate clinical usage, to compared femoral fracture healing at different time points and to explore the effects of Residronate on fracture healing. Methods: Osteoporosis model was achieved by ovariectomy surgery, followed by surgical incision of left femoral shaft 4 weeks after ovariectomy surgery. Three days after fracture surgery, risedronateor saline was fed by intragastric administration. X ray examination was used to check the callus formation, Bone Mineral Density (BMD), Bone Mineral Content (BMC), biomechanical, imaging and micromorphological of bone tissue as well as the trabecular bone parameters were all examined. The femoral pathology tissue of each rat was used to analyze trabecular bone parameters, including trabecular bone volume/tissue volume (Tb. BV/TV), bone surface to tissue volume ratio (BS/TV), trabecular bone mineral density (Tb. BMD), trabecular bone number (Tb. N), trabecular bone thickness (Tb. Th) and small bone Trabecular bone space (Tb. Sp). Results: Via X‐ray and pathologically, risedronate treatment promoted the callus forming at the fracture site during the following 6 weeks after osteoporotic fracture by X‐ray (P < 0.01), increased the local bone mineral density (P < 0.01), and accelerated the fracture healing during the first 3 weeks (P <0.01), but delayed facture healing in the later 3 weeks (P < 0.01). Risedronate increased the bone continuity of fracture at 7th week, but this phenomenon was not found at the 10th week (P < 0.01). Delayed fracture healing occurred locally at the fracture site. At 7th week, Risedronate may promote cartilage cells proliferating at fracture site, increase the dense of bone trabeculae and the connection of bone trabeculae, thicken the bone cortex showing better fracture healing than OPF‐Saline groups (P < 0.01). However, these parameter did not continue during the 7th and 10th weeks. Comparing the first and the later 3 weeks, the rats in group Osteoporotic Fracture‐Risedronate (OPF‐RD) accelerated the local fracture healing in the first 3 weeks but not in the last 3 weeks, which is consistent for the BMD and BMC among each group (P < 0.05). Through evaluation of bone mineral density and bone mineral content, risedronate dramatically increased the BMD at the fracture site and resulted in reduction of BMC by risedronate at the fracture site (P < 0.05) among each group still exist, indicating dramatic (P < 0.05). Through load testing, Risedronate increased the structural strength and mechanical indexes of the new callus (P < 0.01). Conclusion: Risedronate can improve the structural strength and mechanical index of newborn callus. Longer than 7 weeks usage of third generation bisphosphonate of risedronate does not contribute to osteoporotic fracture. [ABSTRACT FROM AUTHOR]

Objective: To determine the asymmetry in the paraspinal muscle before pregnancy and evaluate its association with pregnancy‐associated lumbopelvic pain (LPP). Methods: This was a prospective case–control study conducted from January 2017 and December 2018. A total of 171 subjects (mean age ± SD, 27.4 ± 5.8 years) were finally divided into the LBP group, PGP group, and no LPP group. Each subject was asked to follow a standardized clinical imaging protocol before the pregnancy. The area of muscles (multifidus, erector spinae, and psoas muscles) on the axial slice at mid‐disc of L4–L5 and L5–S1 were segmented and then the cross‐sectional area (CSA) of a particular muscle was measured by outlining the innermost fascial border surrounding each muscle. The mean value of F‐CSA's ratio to T‐CSA (F/T CSA) was used to determine whether the bilateral paraspinal muscle was asymmetrical. Total muscle CSA (T‐CSA) represents the sum of CSA of interested three muscles. The signal intensity can distinguish fat and muscle tissue in a different range. Based on this, functional CSA (F‐CSA), represented by fat‐free area, was evaluated quantitively by excluding the signal of the deposits of intramuscular fat. Total muscle CSA (T‐CSA), functional CSA (F‐CSA), and the ratio of F‐CSA to T‐CSA (F/T CSA) were measured unilaterally and compared between groups. Logistic regression was performed to determine the risk factors for pregnancy‐associated LPP. The Pearson correlation coefficient was performed to test the relationship between asymmetry in F/T‐CSA and pain rating. Results: A total of 124 subjects (72.5%) (28.5 ± 5.2 years) had LPP during pregnancy. Forty‐eight (38.7%) individuals had low back pain (LBP) and 76 (61.3%) had pelvic girdle pain (PGP). Seventy‐six women (44.4%) were determined to have asymmetry in paraspinal muscle according to the definition in this methods section. The duration of follow‐up was 24 months postpartum. A total of 39 (31.5%) women unrecovered from LPP. F/T‐CSA was significantly decreased for LBP in the PGP group than in the and control group (0.03 ± 0.02 vs 0.05 ± 0.03 vs 0.12 ± 0.05, P < 0.001). Meanwhile, significant differences were detected in both groups (all P < 0.001). In patients with LBP, the level of paraspinal asymmetry, represented by the difference in F/T‐CSA, was positively correlated with pain scores (r = 0.52, P < 0.01). However, no statistically significant correlation between pain scores and paraspinal asymmetry was found in PGP (r = 0.42, P > 0.05). Asymmetry in the paraspinal muscle (adjusted OR = 1.5), LBP (adjusted OR = 1.6), LPP in a previous pregnancy (adjusted OR = 1.4), sick leave ≥90 days (adjusted OR = 1.2), and heavy labor (adjusted OR = 1.2) were risk factors for the unrecovered LPP during pregnancy. Conclusions: Asymmetrical muscular compositions could lead to abnormal biomechanics for the segmental motions. Lateral‐directed physical training and stretching may help decrease the occurrence and severity of this condition. [ABSTRACT FROM AUTHOR]

Objective Methods Results Conclusions To investigate the prevalence of autoimmune hepatitis (AIH), primary biliary cholangitis (PBC), and primary sclerosing cholangitis (PSC), and the impact of comorbidity of AIH, PBC, and PSC on hospitalization burden in patients with inflammatory bowel disease (IBD).Inpatients admitted to Peking Union Medical College Hospital from January 1, 1998 to December 31, 2021 were included. Odds ratio (OR) and the corresponding 95% confidence interval (CI) were calculated to compare the risk of AIH, PBC, and PSC between IBD and non‐IBD patients. Medical cost and length of hospitalization were compared between IBD patients with and without AIH, PBC, or PSC.Among the included 858 967 inpatients, there were 3059 patients with IBD. Additionally, there were 117 patients with AIH, 879 patients with PBC, and 35 patients with PSC, regardless of having IBD or not. Patients with IBD had a significantly higher risk of AIH (OR 4.87, 95% CI 1.20–19.71, p = 0.03) and PSC (OR 112.28, 95% CI 53.88–233.98, p < 0.01) than those without IBD. While there was no significant difference in the risk of PBC between patients with and without IBD (OR 1.60, 95% CI 0.67–3.86, p = 0.29). The medical cost of each hospitalization did not differ between IBD patients with and without AIH, PBC, or PSC.IBD patients had a higher risk of AIH and PSC. Comorbidity of AIH, PBC, or PSC has no significant effect on the average cost of each hospitalization in IBD patients. [ABSTRACT FROM AUTHOR]

Dielectric capacitors are widely studied for power supply systems because they can quickly store and release electrical energy. Among various kinds of dielectric materials, antiferroelectrics show promising features of high energy-storage density and efficiency. In this study, epitaxial antiferroelectric PbHfO3 films with different orientations are fabricated, in which remarkable anisotropies of polarization and energy storage properties are discovered. With the optimization of film orientation, much-improved energy density and excellent high-temperature efficiency are achieved in the PbHfO3 films. Moreover, the PbHfO3 films are fabricated onto flexible mica substrates, which exhibit excellent property robustness against mechanical bending. This study provides a fundamental understanding of the anisotropic antiferroelectric behaviors of epitaxial PbHfO3 films and provides a generalizable pathway for flexible energy-storage dielectric capacitors. [ABSTRACT FROM AUTHOR]

Coronavirus disease 2019 (COVID‐19) is the seventh member of the bat severe acute respiratory syndrome family. COVID‐19 can fuse their envelopes with the host cell membranes and deliver their genetic material. COVID‐19 attacks the respiratory system and stimulates the host inflammatory responses, enhances the recruitment of immune cells, and promotes angiotensin‐converting enzyme 2 activities. Patients with confirmed COVID‐19 may have experienced fever, dry cough, headache, dyspnea, acute kidney injury, acute respiratory distress syndrome, and acute heart injury. Several strategies such as oxygen therapy, ventilation, antibiotic or antiviral therapy, and renal replacement therapy are commonly used to decrease COVID‐19‐associated mortality. However, these approaches may not be good treatment options. Therefore, the search for an alternative‐novel therapy is urgently important to prevent the disease progression. Recently, microRNAs (miRNAs) have emerged as a promising strategy for COVID‐19. The design of oligonucleotide against the genetic material of COVID‐19 might suppress virus RNA translation. Several previous studies have shown that host miRNAs play an antiviral role and improve the treatment of patients with COVID‐19. miRNAs by binding to the 3′‐untranslated region (UTR) or 5′‐UTR of viral RNA play an important role in COVID‐19‐host interplay and viral replication. miRNAs interact with multiple pathways and reduce inflammatory biomarkers, thrombi formation, and tissue damage to accelerate the patient outcome. The information in this review provides a summary of the current clinical application of miRNAs for the treatments of patients with COVID‐19. [ABSTRACT FROM AUTHOR]

The association between different clinical states of chronic HBV infection and preterm birth (PTB) is still controversial. A retrospective cohort study among 57,386 pregnant women was conducted to examine the impact of chronic HBsAg positive, both HBsAg and HBeAg positive, and chronic active hepatitis on pregnancy complications related to the overall PTB and its subtypes (spontaneous and iatrogenic). A total of 54,245 pregnancies were included in the final study cohort, among which 2,151(4.0%) pregnant women were HBsAg positive. The PTB rate was 6.0% (129/2151) for HBV‐infected women while 4.5% (2319/52094) for those not. Compared with women not infected with HBV, multivariable‐adjusted analyses showed HBV‐infected women had a 33% higher risk of overall PTB (aRR 1.33 95%CI, 1.11–1.60), a 27% higher risk of spontaneous PTB (aRR 1.27, 95% CI, 1.02–1.57) and a 50% higher risk of iatrogenic PTB (aRR 1.50, 95%CI, 1.07–2.11). The PTB rate was 8.9% (35/395) for both HBsAg and HBeAg‐positive women and 16.2% (22/136) for women with active chronic hepatitis. Multivariable‐adjusted analyses showed women who were both HBsAg and HBeAg positive had a 47% higher risk of overall PTB (aRR 1.47, 95%CI, 1.04–2.09), a 2.03 times higher risk of spontaneous PTB (aRR 2.03, 95%CI, 1.38–2.99) and a 32% higher risk of iatrogenic PTB (aRR 1.32, 95%CI, 0.62–2.81), while women with chronic active hepatitis had a 3.84 times higher risk of overall PTB (aRR 3.84, 95%CI, 2.42–6.10), a 3.88 times higher risk of spontaneous PTB (aRR 3.88, 95%CI, 2.32–6.45) and a 3.01 times higher risk of iatrogenic PTB (aRR 3.01, 95%CI, 1.22–7.44). Different maternal clinical states of chronic HBV infection are independently associated with an increased risk of overall PTB and its subtypes (spontaneous and iatrogenic). [ABSTRACT FROM AUTHOR]

Our knowledge of the coordination of fuel usage in skeletal muscle is incomplete. Whether and how microRNAs are involved in the substrate selection for oxidation is largely unknown. Here we show that mice lacking miR‐183 and miR‐96 have enhanced muscle oxidative phenotype and altered glucose/lipid homeostasis. Moreover, loss of miR‐183 and miR‐96 results in a shift in substrate utilization toward fat relative to carbohydrates in mice. Mechanistically, loss of miR‐183 and miR‐96 suppresses glucose utilization in skeletal muscle by increasing PDHA1 phosphorylation via targeting FoxO1 and PDK4. On the other hand, loss of miR‐183 and miR‐96 promotes fat usage in skeletal muscle by enhancing intramuscular lipolysis via targeting FoxO1 and ATGL. Thus, our study establishes miR‐183 and miR‐96 as master coordinators of fuel selection and metabolic homeostasis owing to their capability of modulating both glucose utilization and fat catabolism. Lastly, we show that loss of miR‐183 and miR‐96 can alleviate obesity and improve glucose metabolism in high‐fat diet‐induced mice, suggesting that miR‐183 and miR‐96 may serve as therapeutic targets for metabolic diseases. Synopsis: miR‐183 and miR‐96 are critical for maintaining the oxidative phenotype of skeletal muscle and whole‐body homeostasis. They regulate fuel selection by coordinating glucose usage and fat catabolism via FoxO1, PDK4 and ATGL in skeletal muscle. Mice lacking miR‐183 and miR‐96 exhibit altered fuel utilization and glucose and lipid homeostasis.miR‐183 and miR‐96 promote glucose usage via FoxO1‐PDK4‐PDH axis in skeletal muscle.miR‐183 and miR‐96 repress intramuscular lipolysis and fatty acid oxidation by targeting ATGL.Loss of miR‐183 and miR‐96 ameliorates the deleterious effects of HFD feeding. [ABSTRACT FROM AUTHOR]

Background and Aims. Magnifying chromoendoscopy (ME-CE) through the observation of pit patterns is a productive way to distinguish between neoplastic and nonneoplastic polyps. Magnifying optical enhancement technology (ME-OE) is an emerging virtual chromoendoscopy imaging technology and appeared to be a promising approach. However, this information is currently not available. This study is aimed at comparing the differential diagnostic value of ME-CE and OE for neoplastic and nonneoplastic polyps. Patients and Methods. Consecutive patients undergoing colonoscopy were randomized (1 : 1) into examination by ME-OE or ME-CE. Histopathological findings were utilized as the reference standard. Accuracy, sensitivity, specificity, and positive and negative predictive values of two endoscopy methods were compared using ME-OE (were classified according to the JNET classification) and ME-CE (were classified according to the Kudo pit pattern classification), respectively, and the time to predict the histological polyp type was compared. And the agreements between the pathological and clinical diagnosis by ME-OE or ME-CE were analyzed. Results. A total of 365 polyps were found in the 220 patients included (ME-OE: 185; ME-CE: 180.202 had nonneoplastic polyps, 163 had neoplastic polyps). The diagnostic accuracy of ME-OE was higher than that of ME-CE (93% vs. 92%, p > 0.05). The average diagnosis time was lower in ME-OE than ME-CE (83 ± 26.4 s vs. 194 ± 17.7 s , p < 0.001). The agreements between the pathological and clinical diagnosis were at least substantial in both groups. Conclusion. ME-OE was superlative to ME-CE in predicting the histology of polyps. OE devoted classification would possibly similarly enhance the endoscopist performance. The trial is registered with ChiCT2000032075. [ABSTRACT FROM AUTHOR]

The complex environment in northern China is the main reason for degradation of expansive mudstone below the canals, which resulted in instability and damage of canal slopes. In this study, a serial of laboratory tests was conducted to explore the volume changes and mechanical behaviors of expansive mudstone below the canals in Xinjiang. The experimental program includes wet-dry (WD) and wet-dry-freeze-thaw (WDFT) tests, volume measurement, and unconfined compression tests. The test results show that during the WD cycles, the volume changes of expansive mudstones with a higher dry range would be more significant. The freeze-thaw process in the WDFT cycles resulted in a decrease of volume change ranges when the expansive mudstones had a relatively smaller dry range and a slight increase of volume change ranges when the expansive mudstones had a relatively larger dry range. In the meantime, the stress-strain relationships of expansive mudstones with different dry ranges all presented strain softening under the cycles of WD or WDFT. The first cycle resulted in a significant decrease of failure strength. After seven WD/WDFT cycles, the failure strength of expansive mudstones with different dry ranges decreased by 37.2%∼59.1%. In addition, the freeze-thaw process in the WDFT cycles promoted the softening of the stress-strain relationships and aggravated the failure strength attenuation of expansive mudstones. Through this study, we expect to provide a preliminary basis for the construction and maintenance of expansive mudstone canals in Xinjiang. [ABSTRACT FROM AUTHOR]

Amnion‐derived prostaglandin E2 (PGE2) and cortisol are key to labor onset. Identification of a common transcription factor driving the expression of both cyclooxygenase‐2 (COX‐2) and 11β‐hydroxysteroid dehydrogenase 1 (11β‐HSD1), the key enzymes in their production, may hold the key to the treatment of pre‐term labor. Here, we have found that the CCAAT enhancer binding protein δ (C/EBPδ) is such a transcription factor which underlies the feed‐forward induction of COX‐2 and 11β‐HSD1 expression by their own products PGE2 and cortisol in human amnion fibroblasts so that their production would be ensured in the amnion for the onset of labor. Moreover, the abundance of C/EBPδ in the amnion increases along with COX‐2 and 11β‐HSD1 at term and further increases at parturition. Knockout of C/EBPδ in mice delays the onset of labor further supporting the concept. In conclusion, C/EBPδ pathway may be speculated to serve as a potential pharmaceutical target in the amnion for treatment of pre‐term labor. [ABSTRACT FROM AUTHOR]

Purpose: Although left atrial (LA) expansion index predicts cardiovascular events, its efficacy for predicting cerebral events is unknown. Methods: This study enrolled 2205 patients who had sinus rhythm after echocardiography in their first visit. LA expansion index was calculated as (Volmax‐Volmin) x100%/Volmin, where Volmax was defined as maximal LA volume and Volmin as minimal LA volume. The study endpoint was ischemic stroke. Stroke subtype was classified as cardioembolic stroke (CE), noncardioembolic stroke with determined mechanism (NCE), or embolic stroke of undetermined source (ESUS). Results: Over a 10‐year (mean 9.7 years) follow‐up period, 128 (5.8%) participants reached endpoint, including 46 with CE, 33 with NCE, and 49 with ESUS. Regardless of stroke subtype, LA expansion index was lower in the event groups compared to the nonevent group. The lowest quartile of LA expansion index was associated with high CHA2DS2‐VASc score at enrollment and more events, including CE, ESUS, atrial fibrillation (AF), heart failure, and all‐cause mortality, relative to other quartiles. The LA expansion index was an independent predictor of CE (HR 0.82; 95% CI 0.723‐0.912, per 10% increase in LA expansion index; P <.0001) and ESUS (HR 0.92; 95% CI 0.881‐0.976, per 10% increase in LA expansion index; p 0.003). An LA expansion index <68% predicts the presence of AF after ESUS with 84% sensitivity and 70% specificity. Conclusion: LA expansion index is useful for predicting CE and ESUS. It is also associated with AF, heart failure hospitalization, and all‐cause mortality. [ABSTRACT FROM AUTHOR]

Introduction: Traditional methods to derive experimentally‐generated relative correction factors (RCFs) for the quantitative analysis of herbal multi‐components by single marker (QAMS) method require reference standards and multiple validations with different instruments and columns, which hampers high throughput implementation. Objectives: To effectively reduce the application amounts of raw material and provide higher and more stable accuracy, this study aimed to develop a method to computationally generate RCFs of herbal components. Materials and Methods: This strategy included the published data collection, calibration curves screening, computer algorithm‐based RCFs generation and accuracy validation. Results: Using the in silico approach, we have successfully produced 133 RCFs for the multi‐component quantitative analysis of 63 widely used herbs. Conclusion: Compared with conventional RCFs, this in silico method would be a low cost and highly efficient way to produce practical RCFs for the QAMS method. Here, an in‐silico based method was developed and successfully applied for the production of RCFs of 63 widely used herbs, which not only solved the problems associated with the absence of rare standard substances but also saved resources to improve work efficiency. This in‐silico method was of great practicability for its high throughput,high accuracy and low cost. [ABSTRACT FROM AUTHOR]

Whether BDNF protein and BDNF mRNA expression of the medial prefrontal cortex (mPFC; cingulated cortex area 1 (Cg1), prelimbic cortex (PrL), and infralimbic cortex (IL)), amygdala, and hippocampus (CA1, CA2, CA3, and dentate gyrus (DG)) was involved in fear of posttraumatic stress disorder (PTSD) during the situational reminder of traumatic memory remains uncertain. Footshock rats experienced an inescapable footshock (3 mA, 10 s), and later we have measured fear behavior for 2 min in the footshock environment on the situational reminder phase. In the final retrieval of situational reminder, BDNF protein and mRNA levels were measured. The results showed that higher BDNF expression occurred in the Cg1, PrL, and amygdala. Lower BDNF expression occurred in the IL, CA1, CA2, CA3, and DG. BDNF mRNA levels were higher in the mPFC and amygdala but lower in the hippocampus. The neural connection analysis showed that BDNF protein and BDNF mRNA exhibited weak connections among the mPFC, amygdala, and hippocampus during situational reminders. The present data did not support the previous viewpoint in neuroimaging research that the mPFC and hippocampus revealed hypoactivity and the amygdala exhibited hyperactivity for PTSD symptoms. These findings should be discussed with the previous evidence and provide clinical implications for PTSD. [ABSTRACT FROM AUTHOR]

BACKGROUND Camptothecin (CPT) and matrine (MAT) have potential as botanical pesticides against several pest species. However, the mechanisms of metabolic and physiological changes in pests induced by CPT and MAT are unknown. In this study, a toxicological test, an NMR‐based metabolomic study, an enzymatic test, and an RT quantitative PCR (RT‐qPCR) experiment were all conducted to examine the effect of CPT and MAT on Spodoptera litura. RESULTS: CPT (0.5–1%) exerted high toxicity against larvae of S. litura and caused growth stagnation and high mortality of larvae. A variety of metabolites were significantly influenced by 0.5% CPT, including several energy‐related metabolites such as trehalose, lactate, succinate, citrate, malate, and fumarate. In contrast, MAT showed low toxicity against larvae and induced almost no changes in hemolymph metabolites of S. litura. Enzymatic tests showed that trehalase activity was significantly decreased in larvae after feeding with 0.5% CPT. RT‐qPCR showed that the transcription levels of alanine aminotransferase, malate dehydrogenase, and isocitrate dehydrogenase were decreased while lactate dehydrogenase was increased in the 0.5% CPT‐treated group. CONCLUSIONS: These data indicate that one of the important mechanisms of CPT against S. litura larvae is via the inhibition of trehalose hydrolysis and glycolysis. Our findings also suggest that CPT exhibits a stronger toxicological effect than MAT against S. litura, which provides basic information for the application of CPT in the control of S. litura or other lepidoptera pests. [ABSTRACT FROM AUTHOR]

Wings are essential for insect fitness. A number of proteins and enzymes have been identified to be involved in wing terminal differentiation, which is characterized by the formation of the wing cuticle. Here, we addressed the question whether chitinase 10 (Cht10) may play an important role in chitin organization in the wings of the fruit fly Drosophila melanogaster. Initially, we first found that Cht10 expression coincides with the expression of the chitin synthase coding gene kkv. This suggests that the respective proteins may cooperate during wing differentiation. In tissue‐specific RNA interference experiments, we demonstrate that suppression of Cht10 causes an excess in chitin amounts in the wing cuticle. Chitin organization is severely disrupted in these wings. Based on these data, we hypothesize that Cht10 restricts chitin amounts produced by Kkv in order to ensure normal chitin organization and wing cuticle formation. In addition, we found by scanning electron microscopy that Cht10 suppression also affects the cuticle surface. In turn, cuticle inward permeability is enhanced in Cht10‐less wings. Moreover, flies with reduced Cht10 function are unable to fly. In conclusion, Cht10 is essential for wing terminal differentiation and function. [ABSTRACT FROM AUTHOR]

Do chronic fluoxetine treatments reduced footshock-induced posttraumatic stress disorder (PTSD) symptoms, including fear and comorbid depression, in the situational reminder phase? Moreover, are the subareas of the medial prefrontal cortex (mPFC), including the cingulate cortex 1 (Cg1), prelimbic cortex (PrL), infralimbic cortex (IL), and basolateral amygdala (BLA), involved in the fluoxetine amelioration of PTSD symptoms? These two crucial issues were addressed in the present study. All mice were injected with chronic fluoxetine or normal saline treatments for the adaptation (14 days), footshock fear conditioning (1 day), and situational reminder (3 days) phases. After adaptation, the mice were subjected to footshock (2 mA, 10 seconds) or nonfootshock and stayed 2 min in a footshock box for 2 min for fear conditioning. Later, they were placed in the footshock box for 2 min in the situational reminder phase. In the final session of the situational reminder phase, a forced swimming test (FST) and immunohistochemical staining were conducted. The results indicated that footshock induced fear and comorbid depression. Meanwhile, chronic fluoxetine treatments reduced fear and depression behaviors. The Cg1, PrL, IL, and BLA were seemingly to increase c-Fos expression after footshock-induced PTSD symptoms in the situational reminder phase. The fluoxetine treatments reduced only the BLA's c-Fos expression. The findings suggest that BLA contributes to the fluoxetine amelioration of PTSD symptoms; however, the mPFC, including the Cg1, PrL, and IL, did not mediate PTSD symptoms' amelioration stemming from fluoxetine. The present data might help us to further understand the neural mechanism of fluoxetine treatments in PTSD symptoms. [ABSTRACT FROM AUTHOR]

Heteroepitaxially flexible oxide systems have been intensely developed and considered as the most promising materials for leading the creation of next‐generation flexible electronic devices. Among them, perovskite manganites have attracted significant attention with their abundant and novel properties such as colossal magnetoresistance (CMR) and metal‐insulator transition. However, the requirement of high quality samples hampers this field, not to mention the advanced nanoengineering. In this study, fluorophlogopite mica (F‐mica) is selected as a flexible substrate to fabricate heteroepitaxial Pr0.5Ca0.5MnO3 (PCMO) with a nanocolumn structure. Through a precise control of thickness, different morphologies are realized to manipulate the magnetotransport properties (reduction of melting field). Moreover, thanks to the excellent flexibility of F‐mica, mechanical modulation of CMR (≈1000%) can be achieved in different flex modes while the magnetic properties remain unaffected. Detailed bending tests are performed to study the behavior of resistive change (≈30%). Through the combination of high flexibility, high quality PCMO, and well‐designed nanocolumn structure, the study exhibits the significant controllability of CMR via mechanical bending, and manifests the potential of such a heteroepitaxially flexible oxide system which can be applied on flexible magnetoresistive devices and sensors. [ABSTRACT FROM AUTHOR]