Search

Background: Leucocyte‐ and platelet‐rich fibrin (L‐PRF) is a blood‐derived biomaterial rich in leucocytes and platelets embedded in a high‐density fibrin network that can be compressed into a membrane and used in surgical applications to stimulate tissue regeneration and wound healing, especially in oral cavity. This study aimed to determine the combined effects of the growth factors and cells present in L‐PRF on fibroblasts that directly face the L‐PRF membranes placed during surgical procedures. Methods: The effect of L‐PRF from six donors on the expression of 84 key wound healing genes in normal human gingival fibroblasts was tested by RT‐qPCR. Results: L‐PRF significantly regulated the expression of 33 fibroblast genes (39%), including interleukins, myofibroblast‐, extracellular matrix‐ and angiogenesis‐associated genes, and matrix metalloproteinase‐1 and −3. L‐PRF regulated fibroblast gene expression both time‐ and dose‐dependently, and the effects were mediated by mitogen‐activated protein kinases ERK1/2, JNK and p38. L‐PRF also stimulated fibroblast wound closure and promoted the ability of fibroblasts to induce endothelial tube formation. L‐PRF‐induced gene expression changes in fibroblast were similar to those observed in early human and pig wounds. Conclusions: This study provides new insights into the biological mechanism by which L‐PRF regulates key gingival fibroblast functions important in wound healing. [ABSTRACT FROM AUTHOR]

Abstract: Aim: The objective of this proof‐of‐concept study was to investigate the effects of a new guided bone regeneration technique with a tissue engineering approach. Materials and Methods: This single cohort observational study evaluated the outcome of the leucocyte‐ and platelet‐rich fibrin (L‐PRF) Block for horizontal bone augmentation in the maxilla. The L‐PRF Block is prepared by mixing a particulated biomaterial with chopped L‐PRF membranes at a 50:50 ratio and adding liquid fibrinogen to glue all together. Horizontal augmentation was assessed linearly and volumetrically immediately after surgery and 5–8 months later by matching consecutive cone beam computed tomography (CBCTs). Results: Ten patients (mean age of 50.7 years [±17.2]) representing 15 sites with horizontal alveolar deficiencies were included. Superimposition of pre‐operative and posthealing CBCT scans showed an average linear horizontal bone gain of 4.6 mm (±2.3), 5.3 mm (±1.2) and 4.4 mm (±2.3), measured at 2, 6 and 10 mm from the alveolar crest, respectively. The volumetric gain was 1.05 cm3 (±0.7) on average. The resorption rate after 5–8 months was 15.6% (±6.7) on average. Conclusions: L‐PRF Block may be a suitable technique to augment deficient alveolar ridges. [ABSTRACT FROM AUTHOR]

Aim To analyse the effect of leucocyte- and platelet-rich fibrin (L-PRF) on bone regeneration procedures and osseointegration. Materials and Methods An electronic and hand search was conducted in three databases (MEDLINE, EMBASE and Cochrane). Only randomized clinical trials, written in English where L-PRF was applied in bone regeneration and implant procedures, were selected. No follow-up restrictions were applied. Results A total of 14 articles were included and processed. Three subgroups were created depending on the application: sinus floor elevation (SFE), alveolar ridge preservation and implant therapy. In SFE, for a lateral window as well as for the trans-alveolar technique, histologically faster bone healing was reported when L-PRF was added to most common xenografts. L-PRF alone improved the preservation of the alveolar width, resulting in less buccal bone resorption compared to natural healing. In implant therapy, better implant stability over time and less marginal bone loss were observed when L-PRF was applied. Meta-analyses could not be performed due to the heterogeneity of the data. Conclusions Despite the lack of strong evidence found in this systematic review, L-PRF might have a positive effect on bone regeneration and osseointegration. [ABSTRACT FROM AUTHOR]