1. [Effects of Bufalin on SYK and CBL family proteins in induction of HL-60 cell apoptosis].
- Author
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Qu XJ, Zhao MF, Liu YP, Teng YE, Qu JL, Zhang Y, Xu L, Li YC, and Hou KZ
- Subjects
- Cell Proliferation drug effects, Down-Regulation, Gene Expression Regulation, Leukemic, HL-60 Cells, Humans, Signal Transduction, Syk Kinase, Apoptosis drug effects, Bufanolides pharmacology, Intracellular Signaling Peptides and Proteins metabolism, Protein-Tyrosine Kinases metabolism, Proto-Oncogene Proteins c-cbl metabolism
- Abstract
The study was aimed to explore the mechanism of SYK and CBL family of ubiquitin ligases in Bufalin-induced HL-60 cells apoptosis. Cell viability was tested by trypan blue staining and apoptosis was detected by using flow cytometry. The expressions of CBL and CBL-b and the phosphorylation of SYK were detected by using immunoprecipitation and Western blot. The results showed that Bufalin inhibited HL-60 cell proliferation in time- and dose-dependent manners. IC(50) of suppressing cell viability at 24, 48 and 72 hours were about 26.3, 7.8 and 2.0 nmol/L respectively. The high dose of bufalin already induced apoptosis of HL-60 cells at 8 hours. SYK was quickly phosphorylated, and the expressions of CBL and CBL-b were down-regulated after treatment with Bufalin. It is concluded that SYK activation and CBL protein down-regulation may be involved in Bufalin-induced HL-60 cell apoptosis.
- Published
- 2009