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258 results on '"Sforza, Stefano"'

32. Protein Quality and Protein Digestibility of Vegetable Creams Reformulated with Microalgae Inclusion.

Author

Prandi, Barbara, Boukid, Fatma, Van De Walle, Simon, Cutroneo, Sara, Comaposada, Josep, Van Royen, Geert, Sforza, Stefano, Tedeschi, Tullia, and Castellari, Massimo

Subjects
SPIRULINA, NUTRITIONAL value, MICROALGAE, CHLORELLA vulgaris, VEGETABLES, DUNALIELLA, PROTEINS
Abstract

Microalgae are considered a valuable source of proteins that are used to enhance the nutritional value of foods. In this study, a standard vegetable cream recipe was reformulated through the addition of single-cell ingredients from Arthrospira platensis (spirulina), Chlorella vulgaris, Tetraselmis chui, or Nannochloropsis oceanica at two levels of addition (1.5% and 3.0%). The impact of microalgae species and an addition level on the amino acid profile and protein in vitro digestibility of the vegetable creams was investigated. The addition of microalgae to vegetable creams improved the protein content and the amino acid nutritional profile of vegetable creams, whereas no significant differences were observed in protein digestibility, regardless of the species and level of addition, indicating a similar degree of protein digestibility in microalgae species despite differences in their protein content and amino acid profile. This study indicates that the incorporation of microalgae is a feasible strategy to increase the protein content and nutritional quality of foods. [ABSTRACT FROM AUTHOR]

Published
2023
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34. Inhibition of DPP-IV Activity and Stimulation of GLP-1 Release by Gastrointestinally Digested Black Soldier Fly Prepupae.

Author

Luparelli, Anna Valentina, Miguéns-Gómez, Alba, Ardévol, Anna, Sforza, Stefano, Caligiani, Augusta, and Pinent, Montserrat

Subjects
HERMETIA illucens, DIGESTION, EDIBLE insects, GLYCEMIC control, BIOACTIVE glasses
Abstract

The beneficial effects of an insect-based diet on human health and, in particular, the regulatory ability of digested insects' proteins on the glycaemic response in humans are topics that need to be investigated deeper. In this work, we performed an in vitro study on the modulatory activity of gastrointestinal digested black soldier fly (BSF) prepupae on the enterohormone GLP-1 and its natural inhibitor, DPP-IV. We verified whether actions intended to valorise the starting insect biomass, i.e., insect-optimised growth substrates and prior fermentation, can positively impact human health. Our results highlight that the digested BSF proteins from all the prepupae samples had a high stimulatory and inhibitory ability on the GLP-1 secretion and the DPP-IV enzyme in the human GLUTag cell line. Gastrointestinal digestion significantly improved the DPP-IV inhibitory capacity of the whole insect protein. Moreover, it was seen that optimised diets or fermentation processes preceding the digestion, in any case, did not positively affect the efficacy of the answer. BSF was already considered one of the edible insects more suitable for human consumption for its optimal nutritional profile. The BSF bioactivity here shown, after simulated digestion, on glycaemic control systems makes this species even more promising. [ABSTRACT FROM AUTHOR]

Published
2023
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40. Chapter Six - Assessing food authenticity through protein and metabolic markers.

Author

Prandi, Barbara, Righetti, Laura, Caligiani, Augusta, Tedeschi, Tullia, Cirlini, Martina, Galaverna, Gianni, and Sforza, Stefano

Abstract

This chapter aims to address an issue of ancient origins, but more and more topical in a globalized world in which consumers and stakeholders are increasingly aware: the authenticity of food. Foods are systems that can also be very complex, and verifying the correspondence between what is declared and the actual characteristics of the product is often a challenging issue. The complexity of the question we want to answer (is the food authentic?) means that the answer is equally articulated and makes use of many different analytical techniques. This chapter will consider the chemical analyses of foods aimed at guaranteeing their authenticity and will focus on frontier methods that have been developed in recent years to address the need to respond to ever-increasing guarantees of authenticity. Targeted and non-targeted approaches will be considered for verifying the authenticity of foods, through the study of different classes of constituents (proteins, metabolites, lipids, flavors). The numerous approaches available (proteomics, metabolomics, lipidomics) and the related analytical techniques (LC-MS, GC-MS, NMR) are first described from a more general point of view, after which their specific application for the purposes of authentication of food is addressed. [ABSTRACT FROM AUTHOR]

Published
2022
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45. Effect of Parmigiano Reggiano Consumption on Blood Pressure of Spontaneous Hypertensive Rats.

Author

Basiricò, Loredana, Morera, Patrizia, Evangelista, Chiara, Galaverna, Gianni, Sforza, Stefano, Prandi, Barbara, Bernabucci, Umberto, and Nardone, Alessandro

Subjects
BLOOD pressure, DIASTOLIC blood pressure, SYSTOLIC blood pressure, HYPERTENSION, RATS, DIETARY supplements
Abstract

In recent years, due to the significant increase in hypertension, peptides which are able to reduce blood pressure have gained special interest by scientific research and food industry. Several bioactive peptides with ascertained ACE-inhibitory activity have been found in Parmigiano Reggiano (PR) cheese and/or mixtures deriving from its digestion in vitro, and this may be predictive of its potential antihypertensive effect in vivo. This study investigated the long-term effect of feeding (PR) cheese on blood pressure (BP) of spontaneously hypertensive rats (SHRs). A total of 30 male SHRs, 13 weeks old, were subdivided into 6 groups balanced for body weight and BP, to receive daily dietary supplementation with: 0.1–0.2–0.4–0.6 g PR/rat, captopril, and water. Systolic and diastolic BP were recorded every two weeks, for 10 weeks. Blood samples were collected at the end of the trial. Dietary integration with PR led to a transitory reduction in rats' pressure in the first 35 days of treatment and pressure decreased in a dose-dependent manner. In the second part of the study, the beneficial effect of PR antihypertensive peptides may have been masked and reduced by the increase in BP of rats linked to the rise in age of animals. No PR derived peptides were detected in rats' serum. Highlights: Parmigiano Reggiano (PR) cheese led to a transitory reduction in rats' pressure in the first 35 days of treatment. This effect was PR dose dependent. The highest amounts of PR tested did not increase both systolic and diastolic blood pressures of hypertensive rats. [ABSTRACT FROM AUTHOR]

Published
2022
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