16 results on '"Shuran Wang"'
Search Results
2. Identification of serum immunoglobulin M (IgM) of crucian carp (Carassius auratus gibelio) and immune response to cyprinid herpesvirus 2 infection
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Shuran Wang, Haotian Wu, Longlong Wang, Pinhua Wang, Liqun Lu, Yousheng Jiang, Ye Zhang, and Dan Xu
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Immunoglobulin M ,Crucian carp ,Polyclonal antibody ,Cyprinid herpesvirus 2 ,Immunoprotection ,Aquaculture. Fisheries. Angling ,SH1-691 - Abstract
Crucian carp (Carassius auratus gibelio), an extensively cultivated freshwater fish, was one of the model species for the study of fish immunology. Polyclonal antibodies were advantageous molecular tools for studying teleost immune system. Specifically, polyclonal antibodies reacting with immunoglobulins (Ig) were used successfully in studies of the teleost fishes. In the present study, we produced polyclonal antibody against CH2 domains of crucian carp IgM, and measured the in vivo dynamics of IgM mRNA caused by CyHV-2 infection. The recombinant protein IgM with relative molecular weight about 53 KD was correctly expressed in prokaryotic cells. The specificity of the polyclonal antibody was evaluated by Western blotting and results revealed that the antibody not only specifically recognized crucian carp serum but also cross-reacted with grass carp serum. Quantitative RT-PCR analysis demonstrated the expression of IgM mRNA changed significantly after CyHV-2 infection. The expression of IgM in the kidney increased and reached a maximum at 6 h post-infection (hpi), while dropped to a low level at 5 days post-infection (dpi). In conclusion, the expression of IgM was significantly upregulated in the kidney of crucian carp infected with CyHV-2, indicating that IgM played a potential role in systemic immunity against viral infection. Polyclonal antibody against crucian carp IgM had certain clinical relevance, which might provide insight into the early stage of virus infection and prevention of the disease.
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- 2024
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3. Synergistic Effects of Nutrients on Musculoskeletal Health in Gerontology: Understanding the Combined Impact of Macronutrients and Micronutrients
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Yupeng Liu, Keyu Qian, Xiaodong Shi, Yuqi Jing, Hangqian He, Yiling Li, Dapeng Li, and Shuran Wang
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osteoporosis ,sarcopenia ,musculoskeletal ,aging ,nutrition ,Nutrition. Foods and food supply ,TX341-641 - Abstract
With the global aging population, addressing prevalent age-related conditions such as osteoporosis and sarcopenia is crucial. Traditional nutritional strategies focusing on single nutrients like calcium, vitamin D, or protein have limitations, prompting a nuanced exploration of the relationship between aging, nutrition, and musculoskeletal health. This cross-sectional study examines the complex interplay between dietary intake of macronutrients, common micronutrients, and water, as well as their association with musculoskeletal health in adults aged 50 to 80 years, using U.S. National Health and Nutrition Examination Survey data (NHANES). Employing multiple linear regression, restricted cubic splines, weighted quantile sum (WQS), and quantile-based g-computation (QGC) regression models, our initial analysis using the WQS model revealed that a one-quartile increase in mixed macronutrient intake was associated with a significant 0.009 unit increase in bone mineral density (BMD) and a 0.670 unit increase in grip strength, while a similar increase in mixed micronutrient intake showed a 0.007 unit increase in BMD and a 0.442 unit increase in grip strength. Our findings highlight the importance of a balanced dietary approach in promoting musculoskeletal health in the elderly, offering holistic strategies for overall well-being.
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- 2024
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4. Determination of Vascular Endothelial Growth Factor-B Concentrations in Aqueous Humor and Plasma of Neovascular Age-Related Macular Degeneration and Polypoidal Choroidal Vasculopathy Patients Before and After Anti-VEGF Therapy
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Huiying Zhou, Xinyu Zhao, Shuran Wang, and Youxin Chen
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Polypoidal choroidal vasculopathy ,Neovascular age-related macular degeneration ,VEGF-B ,Plasma ,Aqueous humor ,Anti-VEGF therapy ,Ophthalmology ,RE1-994 - Abstract
Abstract Introduction Anti-vascular endothelial growth factor (anti-VEGF) injection was widely used in patients with neovascular age-related macular degeneration (nAMD) and polypoidal choroidal vasculopathy (PCV); however, the systemic and local levels of vascular endothelial growth factor (VEGF)-B were seldom detected before. This study was conducted to detect and compare the aqueous humor and plasma VEGF-B levels in nAMD and PCV before and after anti-VEGF therapy. Methods Concentrations of VEGF-B in aqueous humor and plasma of individuals with nAMD (n = 10), PCV (n = 22), and age-related cataract controls (n = 12) were measured by enzyme-linked immunosorbent assay. Ranibizumab was injected intravitreally in patients monthly for three consecutive months. Before each injection in patients and at the baseline of controls, blood and aqueous humor samples were collected. Best-corrected visual acuity (BCVA) and central retinal thickness (CRT) were collected before each injection in patient groups. The differences of BCVA, CRT, and VEGF-B levels in aqueous humor and plasma between groups before and after anti-VEGF therapy were compared. Results VEGF-B was overexpressed in aqueous humor and plasma of nAMD and PCV groups compared with control group (P 0.05). Moreover, there were no obvious difference in levels of VEGF-B in aqueous humor and plasma within the treatment groups after anti-VEGF treatment (P > 0.05). The mean CRT in the nAMD group was thinner than that in the PCV group at baseline (P
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- 2022
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5. An SOC-Based Bidirectional Virtual DC Machine Control for Energy Storage Systems in Offshore Isolated Island DC Microgrids
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Shuran Wang, Zhanshan Wang, Xiuchong Liu, and Xiaolu Ye
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virtual DC machine control ,state of charge balance ,energy storage system ,offshore isolated island DC microgrid ,Naval architecture. Shipbuilding. Marine engineering ,VM1-989 ,Oceanography ,GC1-1581 - Abstract
In order to achieve the state of charge (SOC) balance of distributed energy storage systems (ESSs) in offshore isolated island DC microgrids and enhance the inertia and damping characteristics of DC microgrids, an SOC-based bidirectional virtual DC machine (VDCM) control is presented. The control proposed has the following three improvements. Firstly, the improved VDCM control removes the power loop and torque loop on the basis of ensuring inertia and damping characteristics. Secondly, the inertia time constant and damping constant, which are related to the bus voltage difference and the change rate of bus voltage difference, are introduced to improve bus voltage dynamic performance. The proposed method overcomes the shortcoming in that traditional VDCM control cannot adaptively suppress bus voltage oscillation according to the bus voltage fluctuation amplitude. Thirdly, an improved SOC-based VDCM virtual armature resistance (VAR) is proposed to achieve rapid SOC balance. The improved SOC-based VDCM VAR is an SOC-related function added to the initial VAR. This enables the proposed VAR to have a certain adaptive ability, which can adjust the energy absorbed or released by energy storage units according to the SOC state. Compared with other controls, the control proposed takes less time to achieve SOC balance. Finally, the effectiveness and superiority of the scheme are proven by simulation cases.
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- 2023
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6. The effect of calcium supplementation in people under 35 years old: A systematic review and meta-analysis of randomized controlled trials
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Yupeng Liu, Siyu Le, Yi Liu, Huinan Jiang, Binye Ruan, Yufeng Huang, Xuemei Ao, Xudong Shi, Xiaoyi Fu, and Shuran Wang
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calcium ,peak bone mass ,calcium supplementation ,osteoporosis ,Medicine ,Science ,Biology (General) ,QH301-705.5 - Abstract
Background: The effect of calcium supplementation on bone mineral accretion in people under 35 years old is inconclusive. To comprehensively summarize the evidence for the effect of calcium supplementation on bone mineral accretion in young populations (≤35 years). Methods: This is a systematic review and meta-analysis. The Pubmed, Embase, ProQuest, CENTRAL, WHO Global Index Medicus, Clinical Trials.gov, WHO ICTRP, China National Knowledge Infrastructure (CNKI), and Wanfang Data databases were systematically searched from database inception to April 25, 2021. Randomized clinical trials assessing the effects of calcium supplementation on bone mineral density (BMD) or bone mineral content (BMC) in people under 35 years old. Results: This systematic review and meta-analysis identified 43 studies involving 7,382 subjects. Moderate certainty of evidence showed that calcium supplementation was associated with the accretion of BMD and BMC, especially on femoral neck (standardized mean difference [SMD] 0.627, 95% confidence interval [CI] 0.338–0.915; SMD 0.364, 95% CI 0.134–0.595; respectively) and total body (SMD 0.330, 95% CI 0.163–0.496; SMD 0.149, 95% CI 0.006–0.291), also with a slight improvement effect on lumbar spine BMC (SMD 0.163, 95% CI 0.008–0.317), no effects on total hip BMD and BMC and lumbar spine BMD were observed. Very interestingly, subgroup analyses suggested that the improvement of bone at femoral neck was more pronounced in the peripeak bone mass (PBM) population (20–35 years) than the pre-PBM population (
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- 2022
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7. Effect of high-protein vs. high-fat snacks before lunch on glycemic variability in prediabetes: A study protocol for a randomized controlled trial
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Yupeng Liu, Huinan Jiang, Binye Ruan, Yi Liu, Siyu Le, Xiaoyi Fu, and Shuran Wang
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glycemic variability ,pre-meal ,snack ,high-protein ,high-fat ,randomized controlled trial ,Nutrition. Foods and food supply ,TX341-641 - Abstract
BackgroundChina has the largest number of patients with Type 2 Diabetes Mellitus (T2DM), and it tends to increasingly grow in the future, putting an enormous burden on disease control and prevention in China. While glycemic variability (GV) came to be an important indicator of blood glucose control in diabetic patients, studies suggested that premeal snacks may help blood glucose control, but there are still some problems to be researched. Therefore, we designed this trial to evaluate which kind of premeal snacks would lead to better effects on GV under two diet patterns in pre-diabetes subjects and to evaluate assessments of acceptability and compliance, behavior, and metabolism changes in individuals will be described.Methods and analysisThe study is a single-center, open-label, multiparallel group, randomized controlled trial. A total of 32 male and female volunteers will be randomized into 4 groups in a single allocated ratio of soy milk (powder) snack, milk (powder) snack, almonds snack, and placebo control with 250 ml of water taken 30 min before lunch, respectively. The study consists of two intervention periods over 11 days. The first intervention period under habitual diet conditions from D3 to D6 (4 days), during which all subjects are asked to maintain their habitual eating and daily activities similar to the run-in period. The second intervention consists of prelunch snacks with standard meals. We will examine both the effect of GV and various metabolic and behavioral outcomes potentially associated with the interventions. At the end of this study, we will assess the acceptability and maintainability of the intervention through interviews.Clinical trial registrationChinese Clinical Trial Registry, identifier ChiCTR2200058935.
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- 2022
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8. The F-Box Protein CG5003 Regulates Axon Pruning and the Integrity of the Drosophila Mushroom Body
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Mengying Yang, Yige Guo, Shuran Wang, Changyan Chen, Yung-Heng Chang, and Margaret Su-chun Ho
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F-box ,axon pruning ,mushroom body ,Drosophila ,synaptic structure ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Protein homeostasis serves as an important step in regulating diverse cellular processes underlying the function and development of the nervous system. In particular, the ubiquitination proteasome system (UPS), a universal pathway mediating protein degradation, contributes to the development of numerous synaptic structures, including the Drosophila olfactory-associative learning center mushroom body (MB), thereby affecting associated function. Here, we describe the function of a newly characterized Drosophila F-box protein CG5003, an adaptor for the RING-domain type E3 ligase (SCF complex), in MB development. Lacking CG5003 ubiquitously causes MB γ axon pruning defects and selective CG5003 expression in pan-neurons leads to both γ axon and α/β lobe abnormalities. Interestingly, change in CG5003 expression in MB neurons does not cause any abnormalities in axons, suggesting that CG5003 functions in cells extrinsic to MB to regulate its development. Mass spectrum analysis indicates that silencing CG5003 expression in all neurons affects expression levels of proteins in the cell and structural morphogenesis, transcription regulator activity, and catalytic activity. Our findings reinforce the importance of UPS and identify a new factor in regulating neuronal development as exemplified by the synaptic structure MB.
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- 2021
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9. Selection of the Reference Gene for Expression Normalization in Salsola ferganica under Abiotic Stress
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Shuran Wang and Sheng Zhang
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Salsola ferganica ,abiotic stress ,reference gene ,qRT-PCR ,Genetics ,QH426-470 - Abstract
Salsola ferganica is a natural desert herbaceous plant in the arid area of western and northwestern China. Because of its salt tolerance and drought resistance, it is of great significance in desert afforestation and sand-fixing capacity. There has been much research on the genes involved in plants under desert stresses in recent years. The application of the best internal reference genes for standardization was a critical procedure in analyzing the gene expression under different types. Even so, the reference gene has not been reported in the application of gene expression normalization of S. ferganica. In this study, nine reference genes (TUA-1726, TUA-1760, TUB, GAPDH, ACT, 50S, HSC70, APT, and U-box) in S. ferganica were adopted and analyzed under six different treatments (ABA, heat, cold, NaCl, methyl viologen (MV), and PEG). The applicability of candidate genes was evaluated by statistical software, including geNorm, NormFinder, BestKeeper, and RefFinder, based on their stability values in all the treatments. These results indicated that the simultaneous selection of two stable reference genes would fully standardize the optimization of the normalization research. To verify the feasibility of the above internal reference genes, the CT values of AP2/ERF transcription factor family genes were standardized using the most (ACT) and least (GAPDH) stable reference genes in S. ferganica seedlings under six abiotic stresses. The research showed that HSC70 and U-box were the most appropriate reference genes in ABA stressed samples, and ACT and U-box genes were the optimal references for heat-stressed samples. TUA-1726 and U-box showed the smallest value in gene expression levels of cold treatment. The internal reference groups of the best applicability for the other samples were U-box and ACT under NaCl treatment, ACT and TUA-1726 under MV stress, HSC70 and TUB under PEG treatment, and ACT in all samples. ACT and U-box showed higher stability than the other genes based on the comprehensive stability ranking of RefFinder, as determined by the geometric mean in this study. These results will contribute to later gene expression studies in other closely related species and provide an important foundation for gene expression analysis in S. ferganica.
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- 2022
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10. Structural Features and Potent Antidepressant Effects of Total Sterols and β-sitosterol Extracted from Sargassum horneri
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Donghai Zhao, Lianwen Zheng, Ling Qi, Shuran Wang, Liping Guan, Yanan Xia, and Jianhui Cai
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Sargassum horneri ,sterols ,β-sitosterol ,FST ,TST ,monoamine neurotransmitters ,Biology (General) ,QH301-705.5 - Abstract
The purified total sterols and β-sitosterol extracted from Sargassum horneri were evaluated for their antidepressant-like activity using the forced swim test (FST) and tail suspension test (TST) in mice. Total sterols and β-sitosterol significantly reduced the immobility time in the FST and TST. Total sterols were administered orally for 7 days at doses of 50, 100, and 200 mg/kg, and β-sitosterol was administered intraperitoneally at doses of 10, 20, and 30 mg/kg. β-sitosterol had no effect on locomotor activity in the open field test. In addition, total sterols and β-sitosterol significantly increased NE, 5-HT, and the metabolite 5-HIAA in the mouse brain, suggesting that the antidepressant-like activity may be mediated through these neurotransmitters.
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- 2016
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11. Stenting versus medical treatment for idiopathic intracranial hypertension: a matched- control study.
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Raynald, Hongchao Yang, Xu Tong, Xiaochuan Huo, Xiaoqing Li, Lian Liu, Binbin Sui, Hui Qu, Kehui Dong, Yilong Wang, Shuran Wang, Zhongrong Miao, and Dapeng Mo
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CONFIDENCE intervals ,SURGICAL stents ,TREATMENT effectiveness ,COMPARATIVE studies ,RESEARCH funding ,ODDS ratio ,INTRACRANIAL hypertension ,CRANIAL sinuses ,LONGITUDINAL method ,PAPILLEDEMA ,EVALUATION - Abstract
Background This prospective cohort study compared the outcomes of stenting and medical treatment for patients with idiopathic intracranial hypertension (IIH) and venous sinus stenosis (VSS). Methods In this single- center cohort study, patients with IIH and VSS were evaluated between January 2014 and December 2019 with follow- up periods of 1, 3, and 6 months. The patients received either stenting or medical treatment. The two groups underwent 1:1 matching using propensity score analysis, and the clinical outcomes were compared. Results Following 1:1 matching, 36 patients who underwent stenting and 36 who underwent medical treatment were matched. The median improvements in the papilledema Frisn grade were greater in the stenting group at 1 month (2 vs 0), 3 months (-3 vs -1), and 6 months (-3 vs -1) than in the medical treatment group. Patients who received stenting treatment had a significantly higher prevalence of complete resolution of their respective symptoms (headache, tinnitus, or visual disturbances) at 3 months (58.3% vs 13.9%, OR 8.68, 95% CI 2.74 to 27.52) and 6 months (80.6% vs 22.2%, OR 14.50, 95% CI 4.64 to 45.32) than those receiving medical treatment. Conclusions This matched- control study shows that stenting has a greater efficacy rate and rapid resolution of papilledema and its respective symptoms compared with medical treatment. [ABSTRACT FROM AUTHOR]
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- 2023
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12. Trophodynamics of Organic Pollutants in Pelagic and Benthic Food Webs of Lake Dianchi: Importance of Ingested Sediment As Uptake Route.
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Senrong Fan, Beili Wang, Hang Liu, Shixiong Gao, Tong Li, Shuran Wang, Yong Liu, Xueqin Liu, and Yi Wan
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- 2017
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13. Sulforaphane Attenuates Homocysteine-Induced Endoplasmic Reticulum Stress through Nrf-2-Driven Enzymes in Immortalized Human Hepatocytes.
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Canxia He, Baolong Li, Wei Song, Zhongqing Ding, Shuran Wang, and Yujuan Shan
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- 2014
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14. Correlations of macronutrient-induced functional magnetic resonance imaging signal changes in human brain and gut hormone responses.
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Jie Li, Ran An, Yanping Zhang, Xiaoling Li, and Shuran Wang
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BRAIN ,RADIOGRAPHY ,ANALYSIS of variance ,APPETITE ,BLOOD sugar ,STATISTICAL correlation ,CARBOHYDRATE content of food ,FAT content of food ,HORMONES ,HUNGER ,INSULIN ,MAGNETIC resonance imaging ,NUTRITIONAL requirements ,DIETARY proteins ,RESEARCH funding ,SATISFACTION ,STATISTICS ,DATA analysis ,GHRELIN ,DATA analysis software ,DESCRIPTIVE statistics - Abstract
Background: Body energy homeostasis is largely regulated by the interactions between appetite-related brain regions and gut hormones. Objective: We hypothesized that the sensitivity of appetite-related brain regions [eg, hypothalamus, insula, thalamus, parahippocampal/ hippocampal cortex, caudate, putamen, amygdala, and orbitofrontal cortex (OFC)] varies for each macronutrient, and the differential sensitivity is associated with gut hormone concentrations in humans. Design: Brain activation responses to ingested fat, glucose, protein, and water in the above-mentioned 8 brain regions of 14 healthy men were investigated by using functional magnetic resonance imaging. Fasting and postprandial plasma glucose, insulin, ghrelin, cholecystokinin (CCK), and glucagon-like peptide 1 (GLP-1) concentrations were measured. The relation of the blood oxygen level-dependent (BOLD) signal with plasma glucose and hormone concentrations was assessed by using Pearson's correlation analysis. Results: Ingested macronutrients similarly reduced the BOLD signal in the middle insula, thalamus, parahippocampal cortex, caudate, and lateral OFC. Protein ingestion reduced the BOLD signal in the amygdala more effectively than did fat and glucose ingestion. BOLD signal changes were positively correlated with circulating ghrelin concentrations and were negatively correlated with circulating insulin, CCK, and GLP-1 concentrations. The findings indicate variations in the correlation between brain activation and plasma hormone concentrations after ingestion of different macronutrients. Conclusions: The middle insula, thalamus, parahippocampal cortex, caudate, and lateral OFC, but not the amygdala, have similar sensitivities, to isocaloric and isovolumetric macronutrient solutions. Differential correlations exist between BOLD signal changes in activated brain regions and postprandial changes in plasma concentrations' of different gut hormones in response to the ingestion of different macronutrients. [ABSTRACT FROM AUTHOR]
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- 2012
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15. Improvement in chewing activity reduces energy intake in one meal and modulates plasma gut hormone concentrations in obese and lean young Chinese men.
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Jie Li, Na Zhang, Lizhen Hu, Ze Li, Rui Li, Cong Li, and Shuran Wang
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MASTICATION ,GASTROINTESTINAL hormones ,CHILDHOOD obesity ,CHOLECYSTOKININ - Abstract
Background: Mastication is the first step in ingesting food, but the effects of mastication on energy intake and gut hormones in both obese and lean subjects have not been extensively evaluated. Objective: The current study aimed to compare the differences in chewing activities between obese and lean subjects and to examine the effects of chewing on energy intake and gut hormone concentrations in both obese and lean subjects. Design: Sixteen lean and 14 obese young men participated in the current research. In study 1, we investigated whether the chewing factors of obese subjects were different from those of lean subjects. In study 2, we explored the effects of chewing on energy intake. A test meal consisting of 2200 kJ (68% of energy as carbohydrate, 21% of energy as fat, and 11% of energy as protein) was then consumed on 2 different sessions (15 chews and 40 chews per bite of 10 g of food) by each subject to assess the effects of chewing on plasma gut hormone concentrations. Results: Compared with lean participants, obese participants had a higher ingestion rate and a lower number of chews per 1 g of food. However, obese participants had a bite size similar to that of lean subjects. Regardless of status, the subjects ingested 11.9% less after 40 chews than after 15 chews. Compared with 15 chews, 40 chews resulted in lower energy intake and postprandial ghrelin concentration and higher postprandial glucagon-like peptide 1 and cholecystokinin concentrations in both lean and obese subjects. Conclusion: Interventions aimed at improving chewing activity could become a useful tool for combating obesity. [ABSTRACT FROM AUTHOR]
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- 2011
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16. Protective Effect of Sulforaphane on Human Vascular Endothelial Cells Against Lipopolysaccharide-Induced Inflammatory Damage.
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Yujuan Shan, Ruifang Zhao, Wei Geng, Na Lin, Xiaoxue Wang, Xiaoyan Du, and Shuran Wang
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VASCULAR endothelium ,CARDIOVASCULAR diseases ,BLOOD vessels ,PHOSPHORYLATION ,ENDOTHELINS - Abstract
Sulforaphane (SFN), mainly derived from cruciferous vegetables, has received much attention for its cancer chemopreventive property. Though there have been a few epidemiological studies supporting its beneficial effect on cardiovascular diseases, much experimental evidence are still required to understand its mechanism. In this study, human vascular endothelial cell, a barrier of blood, was used as an in vitro model to investigate the protective effect of sulforaphane on inflammatory damage induced by lipopolysaccharide (LPS). The results showed that sulforaphane inhibited the expression of COX-2 and iNOS stimulated by lipopolysaccharide in a dose- and time-dependent manner. Moreover, sulforaphane suppressed the phosphorylation of ERK1/2, JNK, and p38 activated by lipopolysaccharide. Pretreatment with SB202190, the specific inhibitor of p38, abolished the expression of COX-2 induced by LPS. Likewise, SP600125, inhibitor of JNK, abrogated iNOS expression stimulated by LPS. Moreover, pretreatment with anisomycin (AM), an activator of p38 and JNK, instead of LPS, the expression of COX-2 and iNOS is still inhibited by sulforaphane. Interestingly, SFN significantly induced HO-1 and TR expression down-regulated by LPS. Taken together, these data indicated that sulforaphane exhibited the protective role against the inflammatory injury in vascular endothelia cells, through inactivating p38 MAPK and JNK, as well as inducing phase 2 enzymes. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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