19 results on '"Descours, Ghislaine"'
Search Results
2. Severe bilateral pleuropneumonia caused by Legionella sainthelensi: a case report
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Kamus, Laure, Roquebert, Bénédicte, Allyn, Jérôme, Allou, Nicolas, Valance, Dorothée, Simon, Charles, Jaffar-Bandjee, Marie-Christine, Descours, Ghislaine, Jarraud, Sophie, and Miltgen, Guillaume
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- 2021
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3. Transmission of Legionnaires' Disease through Toilet Flushing
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Couturier, Jeanne, Ginevra, Christophe, Nesa, Didier, Adam, Marine, Gouot, Cyril, Descours, Ghislaine, Campese, Christine, Battipaglia, Giorgia, Brissot, Eolia, Beraud, Laetitia, Ranc, Anne-Gaelle, Jarraud, Sophie, and Barbut, Frederic
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Fragrances (Toiletries) -- Analysis ,Disease transmission -- Analysis ,Ruxolitinib -- Analysis ,Spiramycin -- Analysis ,Genomics -- Analysis ,Health - Abstract
Legionella pneumophila is a gram-negative bacterium usually found in small amounts in water in both nature and built environments. In larger amounts, it can be responsible for a severe pneumonia [...]
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- 2020
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4. Optimized decision algorithm for the microbiological diagnosis of osteoarticular infections in adults using synovial fluid samples: a prospective study in two French hospitals including 423 samples of synovial fluid.
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Dupieux, Céline, Descours, Ghislaine, Verhoeven, Paul, Grattard, Florence, Benito, Yvonne, Vandenesch, François, Cazorla, Céline, Ferry, Tristan, Lustig, Sébastien, Boyer, Bertrand, Boisset, Sandrine, Carricajo, Anne, and Laurent, Frédéric
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- 2024
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5. Hyper-inflammatory profile and immunoparalysis in patients with severe Legionnaires' disease.
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Allam, Camille, Mouton, William, Testaert, Hugo, Ginevra, Christophe, Fessy, Noémie, Ibranosyan, Marine, Descours, Ghislaine, Beraud, Laetitia, Guillemot, Johann, Chapalain, Annelise, Albert-Vega, Chloé, Richard, Jean-Christophe, Argaud, Laurent, Friggeri, Arnaud, Labeye, Vanessa, Jamilloux, Yvan, Freymond, Nathalie, Venet, Fabienne, Lina, Gérard, and Doublet, Patricia
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LEGIONNAIRES' disease ,SEPSIS ,IMMUNITY ,MULTIPLE organ failure - Abstract
Introduction: Severe Legionnaires' disease (LD) can lead to multi-organ failure or death in 10%-30% of patients. Although hyper-inflammation and immunoparalysis are well described in sepsis and are associated with high disease severity, little is known about the immune response in LD. This study aimed to evaluate the immune status of patients with LD and its association with disease severity. Methods: A total of 92 hospitalized LD patients were included; 19 plasmatic cytokines and pulmonary Legionella DNA load were measured in 84 patients on the day of inclusion (day 0, D0). Immune functional assays (IFAs) were performed from whole blood samples collected at D2 and stimulated with concanavalin A [conA, n = 19 patients and n = 21 healthy volunteers (HV)] or lipopolysaccharide (LPS, n = 14 patients and n = 9 HV). A total of 19 cytokines (conA stimulation) and TNF-α (LPS stimulation) were quantified from the supernatants. The Sequential Organ Failure Assessment (SOFA) severity score was recorded at D0 and the mechanical ventilation (MV) status was recorded at D0 and D8. Results: Among the 84 patients, a higher secretion of plasmatic MCP-1, MIP1-β, IL-6, IL-8, IFN-γ, TNF-α, and IL-17 was observed in the patients with D0 and D8 MV. Multiparametric analysis showed that these seven cytokines were positively associated with the SOFA score. Upon conA stimulation, LD patients had a lower secretion capacity for 16 of the 19 quantified cytokines and a higher release of IL-18 and MCP-1 compared to HV. IL-18 secretion was higher in D0 and D8 MV patients. TNF-α secretion, measured after ex vivo LPS stimulation, was significantly reduced in LD patients and was associated with D8 MV status. Discussion: The present findings describe a hyper-inflammatory phase at the initial phase of Legionella pneumonia that is more pronounced in patients with severe LD. These patients also present an immunoparalysis for a large number of cytokines, except IL-18 whose secretion is increased. An assessment of the immune response may be relevant to identify patients eligible for future innovative host-directed therapies. [ABSTRACT FROM AUTHOR]
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- 2023
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6. The clinical presentation of Legionella arthritis reveals the mode of infection and the bacterial species: case report and literature review
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Ibranosyan, Marine, Beraud, Laetitia, Lemaire, Hélène, Ranc, Anne-Gaëlle, Ginevra, Christophe, Jarraud, Sophie, and Descours, Ghislaine
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- 2019
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7. Non-Legionella pneumophila serogroup 1 pneumonia: Diagnosis of a nosocomial legionellosis with the Biofire Pneumonia plus panel
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Courboules, Camille, primary, Dournon, Nathalie, additional, Lawrence, Christine, additional, Noussair, Latifa, additional, Descours, Ghislaine, additional, Sivadon-Tardy, Valérie, additional, Jarraud, Sophie, additional, Herrmann, Jean-Louis, additional, Gaillard, Jean-Louis, additional, Espinasse, Florence, additional, El Sayed, Faten, additional, and Roux, Anne-Laure, additional
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- 2022
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8. Evolution of Bordetella pertussis over a 23-year period in France, 1996 to 2018
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Bouchez, Valérie, Guillot, Sophie, Landier, Annie, Armatys, Nathalie, Matczak, Soraya, Toubiana, Julie, Brisse, Sylvain, Brieu, Nathalie, Hamdad, Farida, Kempf, Marie, Pailhoriès, Hélène, Jensen, Cécile, Lehours, Philippe, Guiraud, Jennifer, Le Bars, Hervé, Isnard, Christophe, Wilhelm, Nathalie, Le Coustumier, Alain, Delmas, Julien, De Briel, Dominique, Souply, Laurent, Aberrane, Saïd, Coudé, Marie, Garnier, Fabien, Descours, Ghislaine, Jean-Pierre, Hélène, Alauzet, Corentine, Gibaud, Sophie-Anne, Bonacorsi, Stéphane, Brasme, Lucien, Lemée, Ludovic, Koebel, Christelle, Lanotte, Philippe, Bland, Stéphane, Petitprez, Hélène, Raffenot, Didier, Levast, Marion, Doucet-Populaire, Florence, Bourgeois-Nicolaos, Nadège, Burucoa, Christophe, Grattard, Florence, Marque-Juillet, Stéphanie, Biodiversité et Epidémiologie des Bactéries pathogènes - Biodiversity and Epidemiology of Bacterial Pathogens, Institut Pasteur [Paris], Centre national de Référence de la Coqueluche et autres Bordetelloses - National Reference Center for Whooping Cough and other Bordetella infections (CNR), Collège doctoral [Sorbonne universités], Sorbonne Université (SU), Département de Pédiatrie et maladies infectieuses [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université de Paris (UP), This work was performed with the financial support of the Institut Pasteur and of Santé Publique France., French pertussis microbiology study group: Nathalie Brieu, Farida Hamdad, Marie Kempf, Hélène Pailhoriès, Cécile Jensen, Philippe Lehours, Jennifer Guiraud, Hervé Le Bars, Christophe Isnard, Nathalie Wilhelm, Alain Le Coustumier, Julien Delmas, Dominique De Briel, Laurent Souply, Saïd Aberrane, Marie Coudé, Fabien Garnier, Ghislaine Descours, Hélène Jean-Pierre, Corentine Alauzet, Sophie-Anne Gibaud, Stéphane Bonacorsi, Lucien Brasme, Ludovic Lemée, Christelle Koebel, Philippe Lanotte, Stéphane Bland, Hélène Petitprez, Didier Raffenot, Marion Levast, Florence Doucet-Populaire, Nadège Bourgeois-Nicolaos, Christophe Burucoa, Florence Grattard, Stéphanie Marque-Juillet, Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Collège Doctoral, Université Paris Cité (UPCité), and Institut Pasteur [Paris]-Institut Pasteur [Paris]
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Serotype ,Bordetella pertussis ,population evolution ,Whooping Cough ,Epidemiology ,[SDV]Life Sciences [q-bio] ,Fimbria ,MESH: Pertussis Toxin ,MESH: Virulence Factors, Bordetella ,Pertussis toxin ,Microbiology ,pertactin ,MESH: Bordetella pertussis ,03 medical and health sciences ,0302 clinical medicine ,Antigen ,Virology ,MESH: Child ,medicine ,Humans ,Virulence Factors, Bordetella ,030212 general & internal medicine ,Child ,Whooping cough ,030304 developmental biology ,Pertussis Vaccine ,vaccine antigen deficiency ,0303 health sciences ,Surveillance ,MESH: Humans ,biology ,MESH: Bacterial Outer Membrane Proteins ,Public Health, Environmental and Occupational Health ,MESH: Whooping Cough ,biology.organism_classification ,medicine.disease ,fimbriae serotype ,3. Good health ,respiratory tract diseases ,Vaccination ,MESH: France ,Pertussis Toxin ,MESH: Pertussis Vaccine ,France ,Pertactin ,Bacterial Outer Membrane Proteins - Abstract
Background Bordetella pertussis is the main agent of whooping cough. Vaccination with acellular pertussis vaccines has been largely implemented in high-income countries. These vaccines contain 1 to 5 antigens: pertussis toxin (PT), filamentous haemagglutinin (FHA), pertactin (PRN) and/or fimbrial proteins (FIM2 and FIM3). Monitoring the emergence of B. pertussis isolates that might partially escape vaccine-induced immunity is an essential component of public health strategies to control whooping cough. Aim We aimed to investigate temporal trends of fimbriae serotypes and vaccine antigen-expression in B. pertussis over a 23-year period in France (1996–2018). Methods Isolates (n = 2,280) were collected through hospital surveillance, capturing one third of hospitalised paediatric pertussis cases. We assayed PT, FHA and PRN production by Western blot (n = 1,428) and fimbriae production by serotyping (n = 1,058). Molecular events underlying antigen deficiency were investigated by genomic sequencing. Results The proportion of PRN-deficient B. pertussis isolates has increased steadily from 0% (0/38) in 2003 to 48.4% (31/64) in 2018 (chi-squared test for trend, p 481 insertion within the prn gene or a 22 kb genomic inversion involving the prn promoter sequence, indicative of convergent evolution. FIM2-expressing isolates have emerged since 2011 at the expense of FIM3. Conclusions B. pertussis is evolving through the rapid increase of PRN-deficient isolates and a recent shift from FIM3 to FIM2 expression. Excluding PRN, the loss of vaccine antigen expression by circulating B. pertussis isolates is epidemiologically insignificant.
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- 2021
9. Legionella antibiotic susceptibility testing: is it time for international standardization and evidence-based guidance?
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Portal, Edward, Descours, Ghislaine, Ginevra, Christophe, Mentasti, Massimo, Afshar, Baharak, Chand, Meera, Day, Jessica, Echahidi, Fedoua, Franzin, Laura, Gaia, Valéria, Lück, Paul Christian, Meghraoui, Alaeddine, Moran-Gilad, Jacob, Ricci, Maria Luisa, Lina, Gerard, Uldum, Søren Anker, Winchell, Jonas, Howe, Robin, Bernard, Kathryn, Spiller, O.B., Chalker, Victoria V.J., Jarraud, Sophie, Portal, Edward, Descours, Ghislaine, Ginevra, Christophe, Mentasti, Massimo, Afshar, Baharak, Chand, Meera, Day, Jessica, Echahidi, Fedoua, Franzin, Laura, Gaia, Valéria, Lück, Paul Christian, Meghraoui, Alaeddine, Moran-Gilad, Jacob, Ricci, Maria Luisa, Lina, Gerard, Uldum, Søren Anker, Winchell, Jonas, Howe, Robin, Bernard, Kathryn, Spiller, O.B., Chalker, Victoria V.J., and Jarraud, Sophie
- Abstract
Legionella pneumophila, a Gram-negative bacillus, is the causative agent of Legionnaire's disease, a form of severe community-acquired pneumonia. Infection can have high morbidity, with a high proportion of patients requiring ICU admission, and up to 10% mortality, which is exacerbated by the lack of efficacy of typical empirical antibiotic therapy against Legionella spp. The fastidious nature of the entire Legionellaceae family historically required inclusion of activated charcoal in the solid medium to remove growth inhibitors, which inherently interferes with accurate antimicrobial susceptibility determination, an acknowledged methodological shortfall, now rectified by a new solid medium that gives results comparable to those of microbroth dilution. Here, as an international Legionella community (with authors representing various international reference laboratories, countries and clinical stakeholders for diagnosis and treatment of legionellosis), we set out recommendations for the standardization of antimicrobial susceptibility testing methods, guidelines and reference strains to facilitate an improved era of antibiotic resistance determination., SCOPUS: ar.j, DecretOANoAutActif, info:eu-repo/semantics/published
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- 2021
10. Macrolide-resistant Bordetella pertussis infection in newborn girl, France
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Guillot, Sophie, Descours, Ghislaine, Gillet, Yves, Etienne, Jerome, Floret, Daniel, and Guiso, Nicole
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Nucleotide sequencing -- Analysis -- Health aspects ,Genes -- Analysis -- Health aspects ,Drug resistance in microorganisms -- Care and treatment -- Analysis -- Health aspects ,DNA sequencing -- Analysis -- Health aspects ,Drug resistance -- Care and treatment -- Analysis -- Health aspects ,Antibacterial agents -- Media coverage -- Analysis -- Health aspects ,Bordetella pertussis -- Analysis -- Health aspects ,RNA -- Analysis -- Health aspects ,Disease transmission -- Care and treatment -- Analysis -- Health aspects ,Erythromycin -- Media coverage -- Analysis -- Health aspects ,Health - Abstract
Bordetella pertussis, the causative agent of whooping cough, continues to circulate among children and adolescents even in regions with high vaccine coverage. Antimicrobial drug treatment contributes substantially to controlling transmission [...]
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- 2012
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11. Adaptation of Amoeba Plate Test To Recover Legionella Strains from Clinical Samples
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Descours, Ghislaine, Hannetel, H., Reynaud, J. V., Ranc, Anne Gaëlle, Beraud, L., Kolenda, C., Campese, C., Lina, Gérard, Ginevra, Christophe, Jarraud, Sophie, Pathogenèse des légionelles- Legionella pathogenesis (LegioPath), Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre National de Référence Legionella, Santé publique France - French National Public Health Agency [Saint-Maurice, France], Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
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Adult ,Aged, 80 and over ,Male ,Microbiological Techniques ,Legionella culture ,Legionellosis ,Time Factors ,Legionnaires' disease ,amoeba plate test ,Sputum ,Legionella ,Bacteriology ,Middle Aged ,Sensitivity and Specificity ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,amoebic coculture ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,Humans ,[SDV.IMM]Life Sciences [q-bio]/Immunology ,Female ,Prospective Studies ,Amoeba ,isolation ,Aged - Abstract
International audience; The isolation of Legionella from respiratory samples is the gold standard for diagnosis of Legionnaires' disease (LD) and enables epidemiological studies and outbreak investigations. The purpose of this work was to adapt and to evaluate the performance of an amoebic coculture procedure (the amoeba plate test [APT]) for the recovery of Legionella strains from respiratory samples, in comparison with axenic culture and liquid-based amoebic coculture (LAC). Axenic culture, LAC, and APT were prospectively performed with 133 respiratory samples from patients with LD. The sensitivities and times to results for the three techniques were compared. Using the three techniques, Legionella strains were isolated in 46.6% (n = 62) of the 133 respiratory samples. The sensitivity of axenic culture was 42.9% (n = 57), that of LAC was 30.1% (n = 40), and that of APT was 36.1% (n = 48). Seven samples were positive by axenic culture only; for those samples, there were \textless10 colonies in total. Five samples, all sputum samples, were positive by an amoebic procedure only (5/5 samples by APT and 2/5 samples by LAC); all had overgrowth by oropharyngeal flora with axenic culture. The combination of axenic culture with APT yielded a maximal isolation rate (i.e., 46.6%). Overall, the APT significantly reduced the median time for Legionella identification to 4 days, compared with 7 days for LAC (P \textless 0.0001). The results of this study support the substitution of LAC by APT, which could be implemented as a second-line technique for culture-negative samples and samples with microbial overgrowth, especially sputum samples. The findings provide a logical basis for further studies in both clinical and environmental settings.
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- 2018
12. KKL-35 Exhibits Potent Antibiotic Activity against Legionella Species Independently of trans -Translation Inhibition
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Brunel, Romain, primary, Descours, Ghislaine, additional, Durieux, Isabelle, additional, Doublet, Patricia, additional, Jarraud, Sophie, additional, and Charpentier, Xavier, additional
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- 2018
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13. Ribosomal Mutations Conferring Macrolide Resistance in Legionella pneumophila
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Descours, Ghislaine, primary, Ginevra, Christophe, additional, Jacotin, Nathalie, additional, Forey, Françoise, additional, Chastang, Joëlle, additional, Kay, Elisabeth, additional, Etienne, Jerome, additional, Lina, Gérard, additional, Doublet, Patricia, additional, and Jarraud, Sophie, additional
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- 2017
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14. Legionella pneumophila et macrolides : de l’antibiogramme à la caractérisation des mécanismes moléculaires impliqués dans la résistance
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Descours, Ghislaine, Pathogenèse des légionelles- Legionella pathogenesis (LegioPath), Centre International de Recherche en Infectiologie - UMR (CIRI), Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Université Claude Bernard - Lyon I, Sophie Jarraud, Christophe Ginevra, STAR, ABES, École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
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Therapeutic failure ,Resistance ,Co-culture amibienne ,Legionella ,23S rRNA ,Résistance ,Ribosome ,Protéines ribosomales L4 / L22 ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Échecs thérapeutiques ,Amoebal co-culture ,Macrolides ,[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,L4 / L22 proteins ,ARNr 23S - Abstract
Macrolides, alone or in combination, are recommended as first-line therapy of Legionnaires’ disease. Legionella pneumophila is characterized by a constant in vitro susceptibility to macrolides. Resistant variants have never been detected, in contrast with several therapeutic failures observed in patients receiving an adequate treatment. Legionellae isolation from respiratory secretions is needed for antibiotic susceptibility testing, but the reported culture sensitivity reaches only 24% in France. In this work, we improved Legionellae isolation rate by culture and amoebal co-culture. In a second time, we were interested in hypothetic mechanisms involved in therapeutic failures like antibiotic antagonisms and acquired resistance. We found no antagonistic effects for macrolide/fluoroquinolones and macrolide/rifampin combinations in an intracellular Legionella model, which made irrelevant our first hypothesis. In a last step, we propagated Legionella strains with increasing macrolides concentrations. This procedure provided us high-level macrolides-resistant mutants. We found that mutations in the genes encoding L4 and L22 proteins were correlated with low-level resistance. Mutations in domain V of the 23S rRNA were associated with intermediate or high-level resistance. We found a correlation between the kind of nucleotide substitution or the number of mutated ribosomal operons and the resistance level. The speed and efficiency of in vitro selection of L. pneumophila macrolide-resistant mutants suggest a potential acquisition of macrolide resistance in patients under therapy and emphasize the need to explore clinical Legionella macrolide susceptibility in order to track down resistance, Les macrolides sont recommandés seuls ou en association dans le traitement des légionelloses. Cependant, malgré une antibiothérapie adaptée et l'absence de souches résistantes décrites, des échecs thérapeutiques sont régulièrement observés. L'isolement de souches de Legionella à partir de prélèvements respiratoires est indispensable à la réalisation d'antibiogrammes mais il n'est obtenu que dans 24% des cas en France. Au cours de ce travail, nous avons tout d'abord amélioré le rendement d'isolement des souches cliniques, notamment par des techniques de co-culture amibienne, avant de nous intéresser aux mécanismes pouvant expliquer les échecs thérapeutiques (antagonisme entre antibiotiques, sélection de résistance). Nous avons pu montrer, dans un modèle de croissance intracellulaire de Legionella pneumophila, une absence d'antagonisme entre macrolides et fluoroquinolones ou rifampicine invalidant la première hypothèse. Nous avons ensuite généré des lignées de mutants hautement résistants aux macrolides. Nous avons mis en évidence des mutations dans les gènes codant les protéines ribosomales L4 et L22 associées à un faible niveau de résistance. Des mutations dans les gènes codant l'ARN ribosomal 23S étaient associées à un niveau plus élevé de résistance, dépendant de la nature de la mutation et du nombre de copies du gène mutées. La facilité d'obtention de mutants résistants aux macrolides in vitro suggère une potentielle acquisition de résistance in vivo au décours de l'antibiothérapie et justifie une recherche systématique de cette résistance dans les prélèvements cliniques
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- 2013
15. Reassessment of the Role of Rapid Antigen Detection Tests in Diagnosis of Invasive Group A Streptococcal Infections
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Gazzano, Vincent, primary, Berger, Anne, additional, Benito, Yvonne, additional, Freydiere, Anne-Marie, additional, Tristan, Anne, additional, Boisset, Sandrine, additional, Carricajo, Anne, additional, Poyart, Claire, additional, Vandenesch, François, additional, and Descours, Ghislaine, additional
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- 2016
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16. Macrolide-ResistantBordetella pertussisInfection in Newborn Girl, France
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Guillot, Sophie, primary, Descours, Ghislaine, additional, Gillet, Yves, additional, Etienne, Jérome, additional, Floret, Daniel, additional, and Guiso, Nicole, additional
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- 2012
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17. Legionella pneumophila Sequence Type 1/Paris Pulsotype Subtyping by Spoligotyping
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Ginevra, Christophe, primary, Jacotin, Nathalie, additional, Diancourt, Laure, additional, Guigon, Ghislaine, additional, Arquilliere, Romain, additional, Meugnier, Hélène, additional, Descours, Ghislaine, additional, Vandenesch, Francois, additional, Etienne, Jerome, additional, Lina, Gérard, additional, Caro, Valérie, additional, and Jarraud, Sophie, additional
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- 2012
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18. Legionella pneumophilaSequence Type 1/Paris Pulsotype Subtyping by Spoligotyping
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Ginevra, Christophe, Jacotin, Nathalie, Diancourt, Laure, Guigon, Ghislaine, Arquilliere, Romain, Meugnier, Hélène, Descours, Ghislaine, Vandenesch, Francois, Etienne, Jerome, Lina, Gérard, Caro, Valérie, and Jarraud, Sophie
- Abstract
ABSTRACTEndemic strains of Legionella pneumophilasequence type 1 (ST1), in particular the ST1/Paris pulsotype, are dispersed worldwide and represent about 10% of culture-proven clinical cases of Legionnaires' disease in France. The high rate of isolation of this strain from both clinical and environmental samples makes identification of the source of infection difficult during epidemiological investigations. The full-length genome sequence of this strain was recently determined, and it revealed the presence of a CRISPR/cascomplex. The aim of this study was to develop and evaluate a spoligotyping tool based on the diversity of this CRISPR locus that would allow the accurate subtyping of the L. pneumophilaserogroup 1 ST1/Paris pulsotype. The CRISPR loci of 28 L. pneumophilaST1/Paris pulsotype isolates were sequenced, and 42 different spacers regions were characterized. A membrane-based spoligotyping method was developed and used to determine the subtypes of 406 L. pneumophilaisolates, including 233 with the ST1/Paris pulsotype profile that were collected in France from 2000 to 2011. A total of 46 different spoligotypes were detected, and 41 of these were specifically identified in the ST1/Paris pulsotype isolates. In 27 of 33 epidemiological investigations, the environmental source of contamination was confirmed by comparing spoligotypes of clinical isolates with those of environmental isolates. With an index of discrimination of 79.72% (95% confidence interval, 75.82 to 83.63), spoligotyping of the L. pneumophilaST1/Paris pulsotype has the potential to be a useful complementary genotyping tool for discriminating isolates with undistinguishable pulsed-field gel electrophoresis (PFGE) and ST genotypes, which could help to identify environmental sources of infection.
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- 2012
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19. KKL-35 Exhibits Potent Antibiotic Activity against LegionellaSpecies Independently of trans-Translation Inhibition
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Brunel, Romain, Descours, Ghislaine, Durieux, Isabelle, Doublet, Patricia, Jarraud, Sophie, and Charpentier, Xavier
- Abstract
ABSTRACTtrans-Translation is a ribosome-rescue system that is ubiquitous in bacteria. Small molecules defining a new family of oxadiazole compounds that inhibit trans-translation have been found to have broad-spectrum antibiotic activity. We sought to determine the activity of KKL-35, a potent member of the oxadiazole family, against the human pathogen Legionella pneumophilaand other related species that can also cause Legionnaires' disease (LD). Consistent with the essential nature of trans-translation in L. pneumophila, KKL-35 inhibited the growth of all tested strains at submicromolar concentrations. KKL-35 was also active against other LD-causing Legionellaspecies. KKL-35 remained equally active against L. pneumophilamutants that have evolved resistance to macrolides. KKL-35 inhibited the multiplication of L. pneumophilain human macrophages at several stages of infection. No resistant mutants could be obtained, even during extended and chronic exposure. Surprisingly, KKL-35 was not synergistic with other ribosome-targeting antibiotics and did not induce the filamentation phenotype observed in cells defective for trans-translation. Importantly, KKL-35 remained active against L. pneumophilamutants expressing an alternate ribosome-rescue system and lacking transfer-messenger RNA, the essential component of trans-translation. These results indicate that the antibiotic activity of KKL-35 is not related to the specific inhibition of trans-translation and its mode of action remains to be identified. In conclusion, KKL-35 is an effective antibacterial agent against the intracellular pathogen L. pneumophilawith no detectable resistance development. However, further studies are needed to better understand its mechanism of action and to assess further the potential of oxadiazoles in treatment.
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- 2017
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