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The consumption of dietary acrylamide (ACR), a carcinogen, results in the dysfunction of various organs and the immune system. However, the impact of ACR exposure on the progression of infectious diseases is unknown. This study investigated the effect of ACR on the progression of malaria infection using a mouse model of malaria. C57BL/6 mice were continuously treated with ACR at a dose of 20 mg/kg bodyweight/day for six weeks (long-term exposure) or phosphate-buffered saline (PBS). Next, the mice were infected with the rodent malaria parasite, Plasmodium berghei NK65 (PbNK). Parasitemia and survival rate were analyzed in the different treatment groups. Magnetic resonance imaging (MRI) and histopathological analyses were performed to evaluate the effect of ACR exposure on the morphology of various organs. Long-term ACR exposure exacerbated PbNK-induced multiorgan dysfunction. MRI and histopathological analysis revealed signs of encephalomeningitis and acute respiratory distress syndrome in the PbNK-infected long-term ACR exposure mice, which decreased the survival rate of mice, but not in the PbNK-infected long-term PBS exposure group. These findings enhance our understanding of the impact of ACR on the progression of infectious diseases, such as malaria.

アンケートをもとに, 病理解剖された臓器の処理問題について考察した.同時に病理検査室における廃棄物・廃液問題や, 剖検を取り巻く法律や医学界の状況についても言及した.多くの施設では剖検臓器を火葬しており, 感染性廃棄物の処理方法として, さらには人道的立場からも適切と思われた.逆にホルマリンは多くの施設で垂れ流し状態であり, 早急な対策が必要であり, その一つとして安価で簡便な消石灰による中和法を紹介した.厚生省の臨床研修病院やいくつかの臨床系学会の認定医制度や認定施設制度では, 一定数以上の剖検施行が要件として挙げられているなど, 病理解剖は病理だけの問題にとどまらない.多方面の関係者の間で剖検を取り巻く諸問題を討議する必要があると思われる.

Olig2 is a recently identified transcription factor involved in the phenotype definition of cells in the oligodendroglial lineage. The expression of Olig2 transcript has been demonstrated in human oligodendroglial tumors, although the protein expression has not been studied extensively. We developed a polyclonal antibody to human Olig2 and analyzed it immunohistochemically. The antibody depicted a single distinct band of predicted molecular weight by Western blotting, and did not cross-react with human Olig1. In normal human brain tissue, the nuclei of oligodendrocytes of interfascicular, perivascular, and perineuronal disposition were clearly labeled by the antibody. Similarly, the nuclei of oligodendroglial tumors were labeled. There was no apparent correlation between the staining intensity and histological grade. Astrocytic components within the tumors were generally less or not stained. Astrocytic tumors were also positive with the Olig2 antiserum to a lesser extent, and the difference between oligodendroglial and astrocytic tumors was demonstrated by a statistical analysis. Olig2 and glial fibrillary acidic protein were expressed in a mutually exclusive manner, and Olig2 expression was cell-cycle related. Neither central neurocytoma nor schwannoma cases were stained. Our antibody was demonstrated to be useful in recognizing normal oligodendrocytes on paraffin sections, and applicable in diagnosis of some brain tumors.

Since 1972, many prosthesis for the knee joint have been reported. They are, however, designed only for hinge and rolling functions neglecting the screw-home function. Surgical damage by massive bone resection would not permit secondary operation even if it is required and only osteosynthesis or hinge prosthesis are indicated thereafter. Musculo-tendinous stability being absolutely required on surgery, ligamentous transfer is often accompanyed in those prosthesis.In view of the above, we deviced a new functional endoprosthesis for the knee joint which satisfies all three functional phases of knee motion and maintains sufficient stability.The functional endoprosthesis (Keio type) is indicated for osteoarthritis, rheumatoid arthritis and some type of condylar fracture. Operative damage by this endoprosthesis is fairly less and secondly reconstruction using any type of similar prosthesis may still remain the treatment of second choice.