1. Analysis of Haemophilus species in patients with respiratory tract infections in Yaoundé, Cameroon
- Author
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Ariane Nzouankeu, Muhamed-Kheir Taha, Eva Hong, Ala-Eddine Deghmane, Eric-Walter Pefura-Yone, Aude Terrade, Véronique Penlap Beng, Marie-Christine Fonkoua, Serges Tchatchouang, Richard Njouom, Mélanie Denizon, Suzie Moyo Tetang Ndiang, Centre Pasteur du Cameroun, Réseau International des Instituts Pasteur (RIIP), Centre National de Référence des Méningocoques et Haemophilus influenzae - National Reference Center Meningococci and Haemophilus influenzae (CNR), Institut Pasteur [Paris], Université de Yaoundé I, Centre Hospitalier Essos [Yaoundé, Cameroun], Jamot hospital, This work was supported by the United States Department of Health and Human Services [grant number 6 DESP060001-01-01], the Institut Pasteur and the Institut Pasteur International Network (RIIP) through Traineeship Grants Calmette and Yersin., and Institut Pasteur [Paris] (IP)
- Subjects
Male ,0301 basic medicine ,Serotype ,MESH: Drug Resistance, Microbial ,Antibiotic resistance ,Antibiotics ,MESH: beta-Lactamases ,Quinolones ,Polymerase Chain Reaction ,0302 clinical medicine ,MESH: Child ,Ampicillin ,Cameroon ,030212 general & internal medicine ,Child ,Respiratory Tract Infections ,MESH: Microbial Sensitivity Tests ,Respiratory tract infections ,Drug Resistance, Microbial ,General Medicine ,Anti-Bacterial Agents ,3. Good health ,Ciprofloxacin ,Infectious Diseases ,Child, Preschool ,Female ,MESH: Haemophilus Infections ,medicine.drug ,Microbiology (medical) ,MESH: Mutation ,Haemophilus Infections ,Adolescent ,Respiratory tract infection ,medicine.drug_class ,030106 microbiology ,Microbial Sensitivity Tests ,Biology ,beta-Lactamases ,lcsh:Infectious and parasitic diseases ,Microbiology ,03 medical and health sciences ,MESH: Anti-Bacterial Agents ,Haemophilus ,Haemophilus species ,medicine ,Humans ,lcsh:RC109-216 ,MESH: Adolescent ,MESH: Humans ,MESH: Quinolones ,MESH: Child, Preschool ,typing ,MESH: Polymerase Chain Reaction ,MESH: Haemophilus influenzae ,MESH: Cameroon ,biology.organism_classification ,rpoB ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Haemophilus influenzae ,MESH: Male ,Typeing ,Whole genome sequencing ,MESH: Respiratory Tract Infections ,Mutation ,MESH: Ampicillin ,MESH: Female - Abstract
Objectives: To identifyHaemophilus species and characterise the antimicrobial susceptibility of isolates from patients with respiratory tract infections (RTIs) in Cameroon. Methods: Isolates (n = 95) were from patients with RTIs obtained from two hospitals in Yaoundé, Cameroon. Isolates were identified by biochemical assay, a polymerase chain reaction (PCR)-based method, matrix-assisted laser desorption ionization-time of flight (MALDI-TOF), and whole genome sequencing. Antibiotic minimum inhibitory concentrations were determined by E-test. Results: Haemophilus influenzae (H. influenzae) was the most prevalent species, varying from 76.8 to 84.2% according to the different methods. The isolates were mainly non-typeable (n = 70, 96%). Three H. influenzae isolates were capsulated (b, e and f). The isolates were genetically diverse and 40 unique sequence types were identified, including 11 new ones. Resistance to ampicillin was observed among 52 of 94 (55.3%), and 14 of the 52 (26.9%) produced TEM-1 β-lactamase. PBP3 mutations occurred in 40 of 52 (76.9%) ampicillin-resistant isolates. Eleven isolates were chloramphenicol-resistant, with eight of 10 (80%) producing chloramphenicol acetyltransferase. Four Haemophilus isolates were rifampicin-resistant, with two mutations in rpoB gene. Five isolates were ciprofloxacin-resistant and harboured mutations in the quinolone-resistance-determining regions of gyrA and parC genes. Conclusion: TheH. influenzae isolates were highly diverse and showed high levels of antibiotic resistance. H. influenzae serotype b is still circulating in the post-vaccination era.
- Published
- 2020