1. IL-33-primed human mast cells drive IL-9 production by CD4 + effector T cells in an OX40L-dependent manner.
- Author
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Battut L, Leveque E, Valitutti S, Cenac N, Dietrich G, and Espinosa E
- Subjects
- Humans, Coculture Techniques, Cells, Cultured, Lymphocyte Activation immunology, Interleukin-13 metabolism, Interleukin-13 immunology, Interleukin-33 immunology, Interleukin-9 metabolism, OX40 Ligand metabolism, Mast Cells immunology, Mast Cells metabolism, CD4-Positive T-Lymphocytes immunology, CD4-Positive T-Lymphocytes metabolism
- Abstract
Interleukin-33 (IL-33) is an alarmin released by epithelial cells in response to tissue damage. It activates resident immune sentinel cells, which then produce signals commonly associated with type 2 immune responses, particularly affecting infiltrating antigen-specific T cells. Given that mast cells (MCs) are a primary target of IL-33 and can shape T helper (Th) cell responses, we investigated the effect of IL-33 priming on the ability of MCs to influence Th cell cytokine production. To examine the Th cell/MC interaction, we developed human primary MC/memory CD4
+ T-cell coculture systems involving both cognate and non-cognate interactions. Our results demonstrated that IL-33-primed MCs, whether as bystander cells cocultured with activated effector T cells or functioning as antigen-presenting cells, promoted IL-9 and increased IL-13 production in Th cells via an OX40L-dependent mechanism. This indicates that MCs sense IL-33-associated danger, prompting them to direct Th cells to produce the key type 2 effector cytokines IL-9 and IL-13., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Battut, Leveque, Valitutti, Cenac, Dietrich and Espinosa.)- Published
- 2024
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