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Abstract The present study was undertaken to know the suppression of weed growth through Dryopteris serrato-dentata (Beddome) Hayata (Dhekishak) and Blumea lacera Dc. (Shialmutra) residues and to estimate the manures and fertilizers effect with the Dhekishakand Shialmutra residues on boro rice at the Agronomy Field Laboratory, Bangladesh Agricultural University, Mymensingh, during the period from November 2022 to April 2023. There were two rice varieties viz., BRRI dhan29 (V1) and BRRI dhan89 (V2) having seven different treatments comprising of residues of Dryopteris serrato-dentata (Beddome) Hayata and Blumea lacera Dc., manures and fertilizers (t ha−1): Control (R1), Residues1.5 + Cowdung 5 (R2), Residues1.5 + Cowdung 2.5 + 50% of recommended dose Fertilizer (R3), Residues 1.5 + Vermicompost 5 (R4), Residues 1.5 + Vermicompost 2.5 + 50% of recommended dose Fertilizer (R5), Residues 1.5 + Trichocompost 10 (R6) and Residues 1.5 + Trichocompost 5 + 50% of recommended dose Fertilizer (R7) and was laid out in a randomized complete block design (RCBD) with three replications. Eleven weed species from seven families were found in this study. Minimum weed growth (1.68) was found in treatment V2R5 (BRRI dhan89 × Residues 1.5 + Vermicompost 2.5 + 50% of recommended dose Fertilizer) and the maximum was (67.40) in V2R1 (BRRI dhan89 × Control). The grain yield as well as the yielding parameters such as the effective tillers hill−1 (8.83), grains panicle−1 (124.98), grain yield (5.33 t ha−1), straw yield (6.49 t ha−1) were recorded highest in V2R5 (BRRI dhan89 × Residues 1.5 + Vermicompost 2.5 + 50% of recommended dose Fertilizer). It was obvious from the study that the residues had capability to suppress the weed growth and integrated manures and fertilizers enhances the yield performance of boro rice. Therefore, Dryopteris serrato-dentata (Beddome) Hayata and Blumea lacera Dc. residues combined with manures and fertilizers might be an efficient source of eco-friendly weed management and yield enhancement tool for sustainable boro rice production.

Abstract Intercropping systems have large heterogeneity in canopy and soil conditions, which could induce phenotypic plasticity. We review different kinds of observed plasticity and to what extent these influence crop performance and outline the diversity of signals that could occur in intercrops. Studying how plasticity is induced and quantifying the consequences for intercrop performance are relevant to better understand the consequences of mixing species and provides leads for breeding for intercrops.

Abstract Ber (Ziziphus mauritiana L.) commonly known as Indian jujube, is an underutilized seasonal fruit rich in nutrients and consumed directly. A punching type mechanism was developed to separate the seed and pulp of ber fruit, based on the analysis of its physical (length, width, thickness measured by vernier caliper) and mechanical properties (cutting and extruding forces measured by texture analyzer). The Pearson correlation coefficient showed that the physical parameters (width, thickness, geometric mean diameter, arithmetic mean diameter) were strongly correlated with the sphericity and surface area of the ber fruits and seeds, whereas length was not significantly correlated. The developed punching type de-seeding mechanism consists of a fruit resting seat, punching rod, guider, crank and lever mechanism, supporting stand, handle, and base. The performance of the developed de-seeder was analyzed, showing fruit pulp wastage from 1.89% to 8.62% and efficiency from 91.38% to 98.11%. The Pearson correlation coefficient indicated that the performance parameters (pulp wastage and efficiency) of de-seeder are negatively correlated. The capacity of the developed de-seeder varies from 15 – 17 kg/ h and 220 – 280 fruits/ h. The developed de-seeder reduces the labour requirement by 67%. In economic evaluation, the de-seeder has reduced the cost of operation (₹ h−1) by 73% and increase the net benefit (₹ year−1) by 6960 over manual operation. Its high efficiency and less pulp wastage make the machine preferable for progressive farmers. Graphical Abstract

Abstract To assess the agronomic performance and genetic stability for grain yield, a set of 36 spring wheat genotypes were grown consecutively for two crop seasons in six environments with varying sowing dates at ICAR-Indian Agricultural Research Institute, New Delhi using randomized complete block design with three replications. Significant genotypic differences were observed for all the studied morpho-physiological characters in both the crop seasons suggesting the presence of substantial genetic variability among the genotypes studied. Parametric and nonparametric stability analysis methods, along with advanced techniques like AMMI and GGE biplot analysis, were employed to understand genotype-environment interactions. Positive correlations were found between yield and stability parameters, highlighting the importance of selecting genotypes with both high yield and stability. Based on the wide-ranging evaluations, three out of 36 genotypes viz., DW1615, HD3262, and DW1631, emerged most promising ones consistently demonstrated superior performance across multiple methodologies. The study underscores the need to follow a multifaceted approach for genotype evaluation to ensure sustainable improvement in wheat production systems, enabling breeders to make informed decisions for resilience and productivity in diverse/varying environmental conditions.

Abstract Flavonoids are often decorated with methyl groups, which are catalyzed by flavonoid O-methyltransferases (FOMTs). Most FOMTs methylate flavonoids in a regiospecific manner. Because of the regiospecific nature of FOMTs, the synthesis of polymethoxyflavonoids is accomplished by multiple O-methylation steps. The multistep synthesis of dimethoxyflavonoids can be efficiently performed by a one-pot procedure using a multienzyme biocatalyst. For the one-pot production of dimethoxyflavonoids, fusion FOMTs were generated by the combination of two different regiospecific FOMTs. RdOMT10 (flavonoid 3-OMT), OsNOMT (flavonoid 7-OMT), and ObFOMT5 (flavonoid 4'-OMT) were used in the FOMT fusion. The fusion FOMTs (OsNOMT/ObFOMT5, OsNOMT/RdOMT10, and ObFOMT5/RdOMT10) were heterologously expressed in Escherichia coli. Activity assays of the recombinant fusion FOMTs demonstrated that OsNOMT/ObFOMT5, OsNOMT/RdOMT10, and ObFOMT5/RdOMT10 catalyze 7/4'-O-methylations, 7/3-O-methylations, and 4'/3-O-methylations of flavonoids, respectively. OsNOMT/ObFOMT5 and OsNOMT/RdOMT10 showed strong dimethylation activity towards diverse flavonoids and were therefore used in the site-selective bioconversion of flavonoids into dimethoxyflavonoids. The E. coli cells bearing OsNOMT/ObFOMT5 successfully converted flavonoids into 7,4'-dimethoxyflavonoids. The engineered E. coli expressing OsNOMT/RdOMT10 converted flavonoids into 3,7-dimethoxyflavonoids. This result indicates that the fusion FOMTs are useful multienzyme biocatalysts for the site-selective production of dimethoxyflavonoids by one-pot bioconversion.

Bu çalışmada, Samsun ili 19 Mayıs ilçesinde çalışma hayatında yer alan kadınların dondurulmuş besin tüketim alışkanlıklarının değerlendirilmesi amaçlanmıştır. Araştırma, 200 çalışan kadın birey ile yapılmıştır. Aktif olarak çalışan ve araştırmaya katılmaya gönüllü olan bireyler çalışmaya dahil edilmiştir. Bireylerin vücut ağırlığı ve boy uzunluğu ölçülmüştür. Bireylerin vücut ağırlığı ve boy uzunluğu kullanılarak beden kütle indeksi (BKİ) hesaplanmıştır. Araştırma sonuçlarında, bireylerin %76,0’sı dondurulmuş besin tüketirken %24,0’ü tüketmemektedir. Dondurulmuş besin gruplarından sırasıyla et ve et ürünleri, meyve ve sebze, hamur işleri daha fazla tüketilmektedir. Bireylerin dondurulmuş besin satın alma nedenleri, %41,4’nün zamandan tasarruf, %48,0’nin hazırlama kolaylığından, %6,6’nın reklamlardan etkilendiği, %18,4’nün mevsimsel farklılıktan satın aldıklarını belirtmişlerdir. Bireylerin satın almama nedenleri incelendiğinde, %25,0’i fiyatını yüksek bulmakta, %33,3’ü sağlıklı olduğuna inanmakta, %33,3’ü taze olmadığı ifade etmekte ve %4,2’i ise doyurucu olmadığını düşünmektedir. Tüketim şekli incelendiğinde, %61,8’i yardımcı yemek olarak, %42,8’i aperatif olarak, %17,1’i ana yemek olarak tükettiklerini belirtmişlerdir. Aile bireyleri içerisinde 15-30 yaş aralığında bireyler dondurulmuş besini en fazla tüketmektedir. Araştırmaya katılan bireylerin dondurulmuş besin tüketip tüketmeme durumuna göre yaş, medeni durum, eğitimleri arasında istatistiksel olarak anlamlı bir farklılık bulunmuştur. Genel olarak dondurulmuş besinler, çalışan bireyler ve aileler için kolaylık, beslenme ve lezzet arasında bir denge sunan kullanışlı ve pratik bir seçenek olabilir. Ancak dondurulmuş besinlerin güvenli etiketlemeye, depolama koşullarına, tüketim miktarına ve şekline de dikkat etmek gerekmektedir.

This research was conducted to determine the presence of Prunus necrotic ringspot virus (PNRSV) and Apple mosaic virus (ApMV) that can cause infection in rose (Rosa spp.) plants grown in Konya province. For this purpose, field and laboratory studies were carried out in areas where roses are mostly grown for landscaping purposes, and infection rates were calculated with the data obtained. The hypothesis of the study is that roses in Konya province may be infected with PNRSV and ApMV and the presence of these viruses can be determined. In line with this hypothesis, various rose growing areas in Konya province were selected as the research area. During the field studies in 2023, guided sampling was carried out and 94 leaf, branch and flower samples were collected from different rose varieties. The collected samples were tested for determining of PNRSV and ApMV infections by Double Antibody Sandwich (DAS) ELISA method, which is one of the serological testing methods in the laboratory. As a result of the tests, it was revealed that the single infections of PNRSV and ApMV were present in 12 and 11 samples of rose plants in Konya province. The total infection rate of both viruses in the province was calculated as 24.47%. Also, PNRSV+ApMV mixed infections were detected in 2 samples. In this study, the infections of PNRSV and ApMV on roses in Konya province were determined for the first time by serological methods. These results will serve as an important source of information for rose producers and agricultural engineers in the region and will allow the development of strategies to control the spread of viruses and minimize infections.

In the arid conditions of the Steppe of Ukraine, the species composition of weeds characteristic of this soil-climatic zone develops. The correctly chosen method of basic soil tillage determines the effectiveness of weed suppression while simultaneously improving the growing conditions for sunflower varieties and hybrids. The purpose of the study was to identify the species composition of weeds in the steppe zone and establish the effectiveness the methods of basic tillage of soil in the technology of growing sunflower hybrids aimed at suppressing weeds and reducing the quantity of weeds in agrocenoses. Research has established that weeds of sunflower agrophytocenoses are represented by 78 species that belong to 2 classes, 18 orders, 27 families, 62 genera. In the agrophytocenosis of sunflower, the largest number of 93.5-96.3% is represented by dicotyledonous weeds (Magnoliopsida). The species composition is dominated by weeds of the Compositae family (Asteraceae) of the dicotyledonous class (Magnoliopsida), which leads to a significant decrease in the yield level. According to the species composition, 3 groups of weeds of the Asteraceae family (Asteraceae) were identified, of which 1 group of weeds is the most harmful and leads to the formation of a minimum yield of sunflower hybrids Yason and Daryi of 1.77-1.79 t/ha. The use of non-moldboard tillage leads to a percentage increase in the share of air dry mass of weeds of the Asteraceae family by 11.1-13.1%, while reducing the yield of sunflower hybrids by 0.13-0.21 t/ha. Thus, with the established species composition of weeds and the quantity of weeds, the use of plowing in sunflower cultivation technology makes it possible to form a maximum yield of 2.07-2.24 t/ha, providing more effective weed suppression in sunflower agrophytocenoses with a minimum percentage of air-dry mass of weeds of the Asteraceae family to the total number of weeds of 21.1-24.1%.

The current study examined the dietary solid-state fermented yellow mealworm (Tenebrio molitor) larvae meal inclusion on growth performance, gut microbiota, body composition, liver and intestinal histology and histomorphometric parameters in the guppy (Poecilia reticulata) for 84 days. Guppies were fed diets included with no supplementation (C); 4 g/kg yellow mealworm larva meal (G1), 4 g/kg solid-state fermented with Lactobacillus brevis yellow mealworm larvae meal (G2), 4 g/kg solid-state fermented with Lactobacillus plantarum yellow mealworm larvae meal (G3), the combination of 2 g/kg solid-state fermented with L. brevis plus 2 g/kg solid-state fermented with L. plantarum yellow mealworm larvae meal (G4). For female guppies, the growth performance of the G4 group clearly differed from all groups with the synergistic effect of solid-state fermented with L. plantarum plus L. brevis. In male guppies, G3 and G4 groups showed the highest growth performance values among all groups. The intestinal microbiota of guppies was clearly varied with supplementation groups. Fusobacteria was the most abundant phylum in C, G1, G2 and G3 groups. However, Proteobacteria showed the most intensity in the G4 group. Intestinal villus height, width and surface area were positively affected in solid-state fermented yellow mealworm larvae meal supplementation groups, reaching higher values in G3 and G4 groups. In conclusion, solid-state fermented yellow mealworm larvae meal via 2 g/kg L. plantarum plus 2 g/kg L. brevis can improve growth performance by modulating the gut microbiota of guppies.

Mosquitoes play a critical role as disease vectors, making them significant in terms of both public health concerns and ecological balance. This study aims to identify mosquito specimens collected from Siirt city center and six different districts using morphological and molecular methods. A 658 bp fragment of the mitochondrial cytochrome c oxidase subunit 1 (COI) gene region was used for molecular diagnosis. The findings revealed four mosquito species: Culex theileri Theobald, 1903, Culex mimeticus Noè, 1899, Culex quinquefasciatus Say, 1823, and Anopheles superpictus Grassi, 1899. Mitochondrial gene PCR products were sequenced, and the sequences were uploaded to the NCBI database for public access. Phylogenetic analysis was conducted using these sequences to investigate the genetic distances and evolutionary relationships among the mosquito species. In the phylogenetic analysis, Chironomus kiiensis was used as an outgroup. The analysis revealed that C. quinquefasciatus and A. superpictus were had the highest genetic distance (0.16), while the closest genetic distance was observed between C. quinquefasciatus and C. theileri (0.06). This study is presented as a preliminary investigation into the genetic diversity, evolutionary relationships, and population dynamics of mosquito species in Siirt Province. Further studies with a larger sample size and additional sequences are needed to establish more comprehensive phylogenetic relationships. The molecular findings contribute significantly to the systematic and ecological studies of mosquitoes.

Bu çalışmanın amacı gebelikte, doğum sonrasında ve emzirme döneminde kullanılan tıbbi ve aromatik bitkiler hakkında genel bilgi vermek ve literatüre katkı sağlamaktır. Çalışmanın sonucunda; Adaçayı (Salvia officinalis L.), Nane (Mentha piperita L.), Melisa bitkisi (Melissa officinalis L.), Mayıs Papatyası (Matricaria chamomilla L.), bitkilerinin yaygın oranda; Ihlamur (Tilia tomentosa Moench), Kekik-Zahter (Thymus serpyllum L.), Isırgan Otu (Urtica dioica L.), Çörek otu (Nigella sativa L.), Badem (Amygdalus communis L.), Zencefil (Zingiber officinale Roscoe) bitkilerinin orta düzeyde; son olarak Ekinezya (Echinacea purpurea (L.) Moench), Sarımsak (Allium sativum L.), Zerdeçal (Curcuma longa L.), Biberiye (Rosmarinus officinalis L.), Sarı Kantaron (Hypericum perforatum L.), Keten tohumu (Linum usitatissimum L.), Ahududu yaprağı (Rubus idaerus L.), Çemen otu (Trigonella foenum-graecum L.) bitkilerinin ise az oranda kullanıldığı belirlenmiştir. Gebelerin, stres düzeylerini azaltmak, bulantı ve kusma gibi sorunlarla başa çıkmak amacıyla bitkisel ürünlere başvurduğu görülmektedir. Doğum sonrası dönemde de, stresin hafifletilmesinin yanı sıra süt üretimini artırmak için bitkisel çözümler tercih edilmektedir. Ancak, bazı bitkisel ürünlerin gebelik ve doğum sonrası dönemde kullanımı ile ilgili dikkat edilmesi gereken önemli noktalar bulunmaktadır. Böyle bitkilerin kullanımı gerek annenin gerekse bebeğin sağlığını olumsuz yönde etkileyebilmektedir. Bu nedenle, gebeler ve emziren annelerin, herhangi bir bitkisel ürün kullanmadan önce mutlaka bir sağlık profesyoneline danışmaları büyük önem taşımaktadır.

Bu çalışmanın amacı sulamalı buğday üretiminde, üretim girdilerinden kaynaklanan toplam sera gazı (GHG) emisyonlarının azaltılması da dahil olmak üzere, sulama yönetimi yoluyla buğday üretiminde enerji verimliliğini artırma ve çevresel etkiyi azaltma potansiyelini değerlendirmektir. Bu kapsamda gerekli olan üretim girdileri ve ürün verimine ilişkin veriler, 2018-2020 döneminde, Konya’da buğdayda tamamlayıcı sulama üzerine yürütülen tarla denemesinden elde edilmiştir. Bu bağlamda beş farklı sulama rejimi dikkate alınmış olup, bunlar: TTS, buğdayın üç kritik gelişme döneminde, 0-90 cm toprak katmanında eksilen nem miktarı kadar sulama; KTS-1, buğdayın üç kritik gelişme döneminde 90’ar mm su ile sulama; KTS-2, buğdayın üç kritik gelişme döneminde 70’er mm su ile sulama; KTS-3, buğdayın üç kritik gelişme döneminde 50’şer mm su ile sulama ve Y, yağışa dayalı konudur. Çalışmadan elde edilen sonuçlara göre, en yüksek dane verimi (7918 kg ha- 1) ve enerji çıktısı (285857 MJ ha-1) TTS uygulamasında elde edilirken, en iyi enerji üretkenliği (0,935 kg MJ-1) ile enerji verimliliği oranı (12,46) ve en düşük çevre kirliliği (2272 kgCO2 eşd ha-1) KTS-3 rejimi altında gerçekleşmiştir. Çalışma kapsamındaki enerji verimliliği ve çevresel kirlilik analizi önemli bulgulara yol açmıştır. Konya gibi tarım arazisi çok, su kaynakları kısıtlı olan bölgelerde, birim alandan (1 ha) yüksek verimin elde edildiği tam sulama (TTS) yerine, aynı ürün miktarının KTS-2 altında 1,04 ha ve KTS-3 rejimi altında ise 1,09 ha’dan üretilebileceği görülmüştür. Bu yolla, ürün miktarında bir azalış olmadan, sulama suyunda %32,4 ile %49 arasında bir tasarruf sağlanırken, sera gazı emisyonu ise %10,3 ile %15,6 arasında azaltılabilecektir.

The date palm (Phoenix dactylifera L.), primarily cultivated in desert regions like the Middle East, is a significant source of nutrition, rich in sugars, polysaccharides, and bioactive compounds such as carotenoids, flavonoids, phenolics, anthocyanins, and sterols. Notably, the polysaccharides in dates exhibit prebiotic properties, supporting beneficial gut microbiota. Research highlights that dates promote the growth of helpful bacteria, including Bifidobacterium and Lactobacillus, which enhance intestinal health, improve barrier function, and increase the production of short-chain fatty acids. Beyond gut health, dates offer additional benefits, including anti-inflammatory, antioxidant, cardiovascular, neuroprotective, and blood sugar-regulating effects. This review synthesizes recent findings on the prebiotic effects and broader health impacts of dates, suggesting their potential in dietary strategies for promoting health and preventing gastrointestinal disorders.

Bu araştırma, melezleme ıslahı yöntemi ile elde edilen 12 melez patates ailesine ait 714 klon arasından uygun özelliklere sahip çeşit adaylarının belirlenmesine, erken dönem seleksiyonlarının katkı sağlaması için yürütülmüştür. Araştırma 113O928 nolu TÜBİTAK-TOVAG projesi kapsamında polikarbon sera şartlarında yürütülmüştür. Araştırma sonucunda melez ailelerinin ortalama çıkış süresi 10,92 gün, bitki boyu, 55,24 cm, ana sap sayısı 1,75 adet olarak belirlenmiştir. Melez aileleri, %97,60 sarı, %2,40’ı kırmızı benekli kabuk rengine sahip iç rengi bakımından ise %75,85’i açık sarı, %13,05’i sarı ve %11,10’u beyaz olduğu belirlenmiştir. Çalışmada yer alan 12 melez ailesinin ocak başına ortalama yumru verimleri 133,93-410,97 g/ocak arasında değişmiş olup, ortalama 267,24 g/ocak şeklinde gerçekleşmiştir. Söz konusu melez ailelerinin bitki başına yumru sayıları 5,99-15,03 arasında değişmiş olup, ortalama 9,97 yumru/ocak olmuş, ortalama yumru ağırlıkları ise 12,58-37,37 g arasında ve ortalaması 24,77 g olarak gerçekleşmiştir. İncelenen 714 klondan 23’ü ele alınan kriterler bakımından daha üstün bulunarak, yapılan erken dönem seleksiyonlarında öne çıkmıştır. Üstün özellik gösteren bu 23 klon, devam eden ıslah süreçlerinin ileri aşamasında ümit var klon olarak değerlendirilmişlerdir.

Arıcılık kendine has özellikleri ile kırsal nüfus için iyi bir alternatif iş ve gelir kaynağıdır. Türkiye ekolojik olarak arıcılığa çok uygun bir doğaya sahip olmasına rağmen maalesef potansiyelinin çok azını kullanmaktadır. Bu çalışma, ekolojik yapısı bakımından arı yetiştiriciliğine uygun alanların fazlalığı, zengin bitki çeşitliğine sahip olması nedeni ile önemli bir konuma sahip olan Erzincan İli Kemaliye ilçesindeki arıcılık faaliyetlerinin mevcut durumunu değerlendirmek amacıyla yürütülmüştür. Bu kapsamda 2023 yılında Erzincan ili Kemaliye ilçesinde faaliyet gösteren 87 arı yetiştiricisi ile tam sayım yapılarak çalışmanın birincil verileri toplanmıştır. Anket çalışmasında 26 soru ile arıcıların demografik özelliklerinin yanı sıra, arıcılığın ekonomik analizi, arıcılık dışı faaliyetleri, üretilen arı ürünleri, bal verimleri, koloni sayıları, ana arı değişimi ile üretimi, hastalık ile zararlılarla mücadeleleri, gezginci arıcılık durumları, besleme şekil ile zamanları, koloni kayıpları, arıcılıkta karşılaştıkları önemli sorunları, pazarlama ve satış faaliyetleri gibi konular hakkında ayrıntılı veriler elde edilmiştir. Araştırma sonuçlarına göre Erzincan ili Kemaliye ilçesinde 1 kg bal maliyeti sabit arıcılarda 141,89 TL, göçer arıcılarda ise 107,20 TL olarak hesaplanmıştır. Kovan başına toplam maliyet sabit arıcılarda 1409,75 TL, göçer arıcılarda ise 1007,83 TL olduğu tespit edilmiştir.

Bu çalışmada Jersey ırkı sığırların süt yağı asit profili ve mevsimsel değişimi incelenmiştir. Araştırmada ilkine buzağılamış 15 baş Jersey ırkı sığır kullanılmıştır. Süt örnekleri yaz ve kış mevsimi olmak üzere iki defa alınmıştır. Yapılan analizler neticesinde mevsimin süt yağının yağ asitleri profili üzerinde sınırlı bir etkisi olduğunu tespit edilmiştir. Sadece 3 yağ asidinde (C21:0, C23:0, C18:1 trans11) mevsimsel değişkenlik gözlemlenmiştir (p

A hybrid camel Tülü (Bactrian male x Dromedary female F1) males are preferred in camel wrestling, which is a culture unique to Anatolia. In this study, changes of live weight (LW), daily weight gain (DWG), and body measurements (BMs) of Tülü calves in the first 6 months of age in a farm in Aydın province, Türkiye, were determined as well as developing equations to estimate LW from body measurements. Tülü calves average birth weight (BW) was 34.7±1.80 kg and reached 175.3±3.38 kg at the age of 6 months with a 0.768±0.03 kg DWG during this time. Although the monthly total weight gains and monthly DWG averages of the calves in the first 6 months were similar, the changes in monthly LW and BMs were statistically significant (P

Molecular approaches used to identify bacterial species use 16S rRNA and MLST to determine the genetic linkage of bacteria; MLST characterizes clonal linkages by examining differences in various gene loci. MLVA determines the genetic relationships of bacterial strains and biovar-level differences and assesses the copy number of repeated DNA sequences. Sequencing provides genetic data by identifying DNA sequences; Sanger sequencing is the basis for next-generation approaches. CRISPR modifies the genetic code and can correct mutations or control genes using Cas9. These methods are important for identifying bacterial species and annotating genomic information. The methods used for this purpose are brought together in this study. The explanation and detailed description of the methods examined will contribute to their use in the field of microbiology.

This study aimed to evaluate the biochemical and nutritional profiles of three cauliflower cultivars—Valentena, Carotena, and Snow White—focusing on chlorophyll, carotenoids, anthocyanins, vitamin C, flavonoids, phenolics, and antioxidant activity. Uniform curds were harvested at 60 days post-sowing from Baratia, Dumuria, Khulna, and analyzed at Khulna Agricultural University. Valentena exhibited the highest chlorophyll content (40.06±0.39 µg/100g FW chlorophyll a, 28.98±3.35 µg/100g FW chlorophyll b), superior lycopene (8.71±0.38 µg/100g FW) levels. Carotena showed the highest total carotenoid content (60.52±1.76 µg/100g FW) and β-carotene (26.99±0.44 µg/100g FW), while Snow White had the lowest values across most parameters. Valentena also led in anthocyanins (101.56±3.9 mg/L FW) and total flavonoids (79.56±10.36 mg/100g FW), with Carotena having the highest vitamin C content (60.05±2.93 µg/g FW). DPPH assays indicated that Valentena showed the most effective antioxidant (IC50 = 43.65±3.56 mg/mL FW), followed by Carotena and Snow White. Hierarchical clustering and pricipal component analysis (PCA) revealed distinct biochemical profiles: Valentena and Carotena shared similarities in carotenoids and antioxidant activity, whereas Snow White differed significantly. Linear discriminant analysis identified lycopene, chlorophyll b, and β-carotene as major differentiators, highlighting the diverse nutritional and antioxidant properties of these cauliflower varieties. The findings highlight the potential of Carotena and Valentena for health-conscious consumers seeking nutrient-rich, antioxidant benefits in functional meals.

This study was conducted in Pokhara, Kaski, Nepal in 2023 to investigate the effects of different pinching types and okra varieties on various growth and yield parameters. Treatments were arranged under a two-factor Randomized Complete Block Design (RCBD) with three replications. The treatments includes two okra varieties (Arka Anamika and Parbati) and 4 pinching types (apical bud pinching (P1), ABP along with 1 leaf pinching (P2), and ABP along with 2 leaf pinching (P3) and control (P4)). Parbati has exhibited a higher plant height (80.78 cm) than Arka Anamika (72.35 cm). Similarly, the P4 pinching type resulted in the tallest plant (85.91 cm). The P1 pinching type demonstrated the highest number of primary branches (4.87), while the control plots had the lowest (3.00). Arka Anamika showed a higher leaf count (40.77) than Parbati (37.19). Notably, the P1 recorded the highest leaf count (43.41), followed by P2 (37.19), with the control plots showing the lowest leaf count (32.76). The findings reveal the significant impact of pinching treatments on yield. Pinching type P1 produced the highest yield of 15.45 mt/ha, whereas the control group yielded the lowest at 9.31 mt/ha, which was comparable to the yield observed for pinching type P3 at 10.83 mt ha-1. P1 also exhibited the highest number of pods per plant (15.90). Varieties and pinching methods exhibited notable interactions in average pod weight, diameter, and length. P1 displayed the widest pods (5.97 cm), whereas P2 had the longest (13.18 cm). Additionally, it can be noted that P3 yielded the heaviest pods at 16.16g when compared to P2, which yielded 14.09 g. Pinching treatments significantly influenced number of days to flowering, with P3 demonstrating the longest duration. Economic analysis was performed for evaluating technical efficiency, facilitating informed and sustainable decisions. Economically, P1 demonstrated superior performance, yielding a higher gross return of NPR 540,808.3, a net return of NPR 418,708.3, and a benefit-cost ratio of 4.43.

In this study, the fruits of Pyracantha coccinea, known for their ornamental and medicinal properties, were analysed to evaluate their antioxidant capacity, mineral content, and heavy metal concentrations. The antioxidant potential of Pyracantha coccinea was determined using DPPH, CUPRAC, and ABTS tests. Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES), Atomic Absorption Spectroscopy (AAS), and Gerthard Dumatherm techniques were used to determine the mineral and nutrient composition of the plant. As a result of the evaluation, calcium (0.25±0.02%), protein (4.29±0.47%), potassium (0.39±0.01%), magnesium (0.197±0.01%), sodium (0.08±0.01%), iron (0.012µg/g DW), aluminium (138±9.6 µg/g DW), cobalt (0.541±0.11 µg/g DW), chromium (0.422±0.05 µg/g DW), manganese (20±1.7 µg/g DW), zinc (43.9±4.6 µg/g DW), % DPPH (76.92±0.48) % ABTS value (77.52±0.39) and CUPRAC values (0.771±0.045 for 100ppm) were determined. In particular, the high levels of chromium (Cr) and zinc (Zn) in the fruits exceed the thresholds considered safe for medicinal applications and suggest that the heavy metal content in plants for medicinal use should be critically evaluated within acceptable limits. This study aims to explore the nutritional value and safety of Pyracantha coccinea by examining its antioxidant properties, mineral content, and potential heavy metal contamination. The findings will help shed light on its potential benefits and risks, offering valuable insight for its use in health, nutrition, and environmental applications.

This research was carried out during the 2022 and 2023 growing seasons on Agricultural Research and Application Fields of Tokat Gaziosmanpaşa University. The study aimed to investigate the effects of different organic fertilizers and application rates on the yield and yield-related characteristics of black cumin (Nigella sativa L.). The experiment was designed using a split plot arrangement in a randomized complete blocks design with three replications. The main plots were assigned to five organic fertilizer types: Leonardite, Vermicompost, Solid Humus, Chicken Manure and Sheep Manure, while the subplots included in the same way in all fertilizer forms four fertilizer doses (0, 150, 300 and 600 kg/da). The study utilized “Çameli” variety, the only registered Nigella sativa L. variety in Turkiye. Key parameters examined over both years included plant height (cm), number of branches per plant (number), number of capsules per plant (number), number of grains per capsule (number), thousand seed weight (g), seed yield (kg/da). The results; revealed that the average plant height ranged from 39.76 to 54.33 cm, the number of branchers per plant, ranged from 6.22 to 6.35, the number of capsules per plant ranged from 13.43 to 14.88, the number of seed per capsule ranged from 98.56 to 104.00, thousand seed weight varied between 2.50 and 3.11 g, seed yield ranged from 135.26 to 135.41 kg/da. Statistically significant differences (p

Çalışmada, Japon bıldırcınlarının (Coturnix coturnix Japonica) yemlerine farklı oranlarda kekik yağı ilavesinin yumurta kalite parametreleri üzerindeki etkileri incelenmiştir. Çalışma, 6 haftalık yaşta 72 dişi Japon bıldırcını üzerinde gerçekleştirilmiştir ve bıldırcınlar kontrol, düşük kekik yağı (150 mg/kg) ve yüksek kekik yağı (300 mg/kg) gruplarına ayrılmıştır. Rasyona farklı oranlarda kekik yağı eklenmesinin bazı yumurta dış ve iç kalite parametreleri üzerine etkileri istatiksel olarak incelenmiştir. Yapılan tek yönlü varyans analizi (ANOVA) sonucundayumurta kabuk oranı, şekil indeksi ve sarı rengi gibi parametrelerin gruplar arasında istatiksel olarak anlamlı farklılıklar gösterdiği belirlenmiştir (P

This study aimed to investigate the antioxidant and enzyme inhibitory properties of Muscari armeniacum, a plant native to Türkiye, which is known for its bioactive compound content. Ethanol extracts of Muscari armeniacum were prepared and analyzed using a variety of bioanalytical methods to assess its potential health-promoting properties. The antioxidant capacity was evaluated through Fe³⁺-Fe²⁺ reduction capacity, CUPRAC, DPPH, and ABTS radical scavenging activities. Additionally, the total phenolic and flavonoid contents of the extracts were determined, as these compounds are critical indicators of antioxidant potential. The antioxidant activity of Muscari armeniacum was compared with five standard antioxidants commonly used for benchmarking: BHA, α-tocopherol, BHT, trolox, and ascorbic acid. The extract demonstrated a high flavonoid content (23.24 µg QE/mg extract) and a significant amount of phenolics (14.38 µg GAE/mg extract), with the flavonoid content being particularly noteworthy. In addition to its antioxidant properties, the enzymatic activity assays revealed that the extract significantly inhibited acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes, which are associated with neurodegenerative diseases such as Alzheimer’s. The IC₅₀ values were determined to be 54.14 mg/mL (R² = 0.9695) for AChE and 58.73 mg/mL (R² = 0.9609) for BChE, indicating moderate enzyme inhibition. These results suggest that Muscari armeniacum could serve as a valuable source of bioactive compounds with significant antioxidant and enzyme inhibitory activities. Its potential applications in the treatment of oxidative stress-related diseases and neurodegenerative disorders make it a promising candidate for further pharmacological studies. This research underscores the importance of exploring native plants for their medicinal and therapeutic potential, contributing to the development of alternative treatments for challenging health conditions.

Population growth has increased demand and diversified use of Irish potato which have increased its demand. The crop has become a major source of food and income for many households across the world. However, despite the high potential of about 30 tonnes per hectare (ha), smallholder farmers in Kenya realize low Irish potato yields ranging from 4-8 tonnes per ha due to limited uptake of agricultural technologies. The low yields calls for a profound understanding of the factors influencing the uptake agricultural technologies. The study analyzed the effects of the adoption of agricultural technologies on Irish potato yield in Ol Kalou Sub County. The study considered chemical fertilizer, certified seeds, fungicides, and farm machinery as the four main agricultural technologies that that affect yield. A descriptive cross-sectional research design was used to obtain data from a study population of 21,942 smallholder Irish potato farmers in Ol Kalou Sub County. A multiple-stage sampling technique was employed to generate a sample size of 385 respondents who provided primary data. Data collected was analyzed using endogenous switching regression model using STATA version 17. The study found that the average treatment effect on treated (ATT) was 10.21 bags per acre. In addition, the Endogenous switching regression model showed that the expected yield for the adopter increased by 51.83%. Out of the four technologies, the use of chemical fertilizers had the highest effect of 37% on yield. The study concluded that the adoption of agricultural technologies increases the yield of Irish potatoes. The study recommends that national and county governments should develop policy regulations such as training and extension services, market access, price support and public-private partnerships encouraging farmers to uptake agricultural technologies. The study also recommends that both levels of government should subsidize agricultural technologies, hence reducing the cost of adoption.

Yerel geleneksel yemekler bölgelerin tarihsel, coğrafi ve kültürel özelliklerini yansıtırken, bireylerin yeme alışkanlıkları ise sosyal ve ekonomik yapıya dair ipuçları sunar. Günümüzde, küresel endüstriyel gıda üretimi ve dağıtımı karşısında geleneksel gıda üretimi ciddi bir rekabet gücü kaybı yaşamaktadır. Geleneksel tarımsal ürünlerin üreticisi olan çiftçiler, yok olma tehlikesiyle karşı karşıyadır. Bu durum hem geleneksel ürünlerin kaybı hem de yeme-içme kültürümüzün önemli bir kısmının yok olması anlamına gelmektedir. Avrupa Birliği (AB) bu kaybın önlenmesi ve sorunun tüm boyutları ile değerlendirilip çözümü için kısa adı TRUEFOOD olan büyük bir proje yürütmüştür. Bu proje kapsamında AB, geleneksel gıdaların küresel rekabet koşullarında korunmasını sağlarken, üretim süreçlerinin çağın gerektirdiği gıda güvenliği standartlarına uyumlu hâle getirilmesini hedeflemiştir. Türkiye’de ise bu sürece parelel olarak başlatılan ve yaklaşık 20 yıldır düzenlenen Geleneksel Gıdalar Sempozyumu (GGS) serisi ile geleneksel gıdalarımız kayıt altına alınmaya çalışılmaktadır. Ayrıca çeşitli kurumlar AB’de olduğu gibi Coğrafi İşaret (Cİ) tescilleri ile “geleneksel gıdaları” korumaya çalışmaktadır. GGS serisinde sunulan birçok makalede, geleneksel gıdaların sadece ham madde ve üretim teknolojilerinden ibaret olmadığı; bununla birlikte toplumsal kimlik ifadesi, dini ritüel, dayanışmayı teşvik eden bir olgu ve bireylerin hatıralarını yansıtan bir bütün olduğu görülmektedir. Serinin gelecek sempozyumlarında ise “geleneksel gıdalarımızın” kayıt altına alınmasında onların hikâyelerinin ve sosyolojik boyutlarının birlikte inceleneceği bir gelenek oluşturulmasının kültürümüzün devamlılığı açısından büyük yararlar sağlayacağı düşünülmektedir.

Tarımsal mekanizasyon, tarım alanlarını geliştirmek, her türlü tarımsal üretimi yapmak ve ürünlerin işlenmesini de gerçekleştirmek amacıyla kullanılan tüm enerji kaynağı, mekanik araç ve gerecin tasarımı, yapımı, geliştirilmesi, pazarlanması, yayım ve eğitimi, işletilmesi ve kullanılması konularını içermektedir (Zeren ve ark., 1995). Tarımsal mekanizasyon göstergeleri kullanılarak tarımsal mekanizasyon düzeyinin belirlenmesi, yörelerin ve ülkelerin tarımsal mekanizasyon seviyelerinin ortaya konmasında önem arz etmektedir. Bu çalışmada Kırşehir ili Çiçekdağı ilçesinin tarımsal mekanizasyon özellikleri incelenmiştir. Çalışmada, Türkiye İstatistik Kurumu’nun (TUİK) 2023 ve 2024 yıllarına ait verileri ile Çiçekdağı İlçe Tarım Müdürlüğü’nden elde edilen veriler materyal olarak kullanılmıştır. Bu kapsamda Çiçekdağı ilçesinin tarım alet ve makine sayıları (traktör sayısı, biçerdöver sayısı, birinci ve ikinci sınıf toprak işleme alet ve makine sayısı, ekim-dikim ve bakım makinesi sayısı, bitki koruma makinesi sayısı ve hasat-harman makinesi sayısı) da çalışmaya dahil edilmiştir. Mekanizasyon düzeyi; traktör, biçerdöver, tarım aletleri sayısı tespit edilmiş olup ve tarımsal mekanizasyon düzeyi göstergeleri; traktör/1000 ha, ha/traktör, kW/ ha ve ortalama traktör gücü (kW) olarak belirlenmiştir.Verilere göre, traktör sayısı 1000 hektar başına 35,97’den 38,74’e yükselmiş olup , traktör başına düşen arazi alanı ise 27,79 hektardan 25,81 hektara gerilemiştir. Traktör başına düşen güç 2,49 kW/ha’dan 2,68 kW/ha’ya artarken, ortalama traktör gücü 69 kW seviyesinde sabit kalmıştır.

Soil pH, governed by the relative concentrations of hydrogen () and hydroxyl () ions, is a key factor affecting the chemical, physical, and biological properties of soils. Most soils in Türkiye are alkaline due to calcareous parent material and climatic influences, which restricts the availability of essential nutrients to plants. Sulphur applications are widely employed to reduce soil pH and increase nutrient bioavailability. The use of Sulphur for the amelioration of alkaline soils will continue to be a crucial strategy for enhancing agricultural sustainability in the future. This study investigates the effects of different Sulphur sources on the pH of sandy and clay-loam texture soils. This study investigates the effects of different Sulphur sources on the pH of sandy and clay loam textured soils. The soil samples used in the research were taken from Pınarbaşı and Melikgazi districts of Kayseri province, and soil samples were taken from both regions from a depth of 30 cm and from 20 randomly determined different points. Sulphur applications were applied at rates of (0, 0.02, 0.04 g 100 g-1) (X: powdered Sulphur ) and (0, 0.044, 0.088 g 100 g-1) (Y: granular Sulphur ) based on weight for clay-loam and sandy textured soils, respectively. Samples taken on days 0, 15, 30, 60, 90, 180, and 360 post-applications showed that the impact of Sulphur applications on soil pH change was significant across all treatments (p

The most important factor in both plant development and productivity, which mostly depends on nutrient availability in the soil and environment, is the constituents of soil nutrients. Soil is always losing its nutrients by leaching, surface runoff as well as through harvesting and cultural operations like: pruning. For maintaining the balance of agricultural ecosystem fertilizer application is necessary to improve soil condition and gain better yield. In case of tea industry for maintaining better and uniform production applying fertilizer is a widespread management technique in worldwide. Tea requires a lot of macro and micronutrients for growth because it cannot be grown without the usage of fertilizer and other nutrient supplies. The purpose of this field survey was to ascertain the nutritional condition of tea leaves as well as the signs of nutrient deficiencies in tea plants. In this study, deficiency symptoms of the essential nutrients were found out at different tea estates of Moulvibazar and Sylhet districts as well as photographs were taken. Photographs of the nutrient deficient tea plants were correlated with the established symptoms. The range of total nitrogen, phosphorus, potassium, calcium, magnesium and zinc content in the collected tea leaf samples were 2.95- 5.18%, 0.28- 0.49%, 0.56-1.88%, 0.12-0.49%, 0.07-0.08% and 0.002- 0.004%, respectively.

The study is aimed at investigating future changes in sustainability of olive farming by means of climate change, and changes in agricultural climatic suitableness and phenology of olive tree cultivation in the North Aegean sub-region of Türkiye mainly characterised with dry summer subtropical Mediterranean climate. According to projected changes based on RCP8.5 scenario, projected warming reaches an average of 5-6°C increase indicating most negative condition on olive cultivation. According to RCP8.5 scenario annual precipitation projections, present suitable areas of olive groves will decrease in the period of 2049-2073, and almost the entire study area will be in the category of medium suitable. With respect to projected Emberger Bioclimate classification, for RCP 8.5 scenario, there will be a significant increase in dry-sub humid areas in the period of 2049-2073. This increase will cover up the coastal areas in the period of 2074-2098, and even all the study region will be very likely characterised with dry-sub humid and semi-arid Mediterranean bioclimatic types except for some coastal areas. An increase of about 6°C is expected in maximum values of maximum air temperatures during the bud swelling periods in the spring, especially after 2050 under RCP8.5 scenario. This increase in extreme maximum temperatures may cause olive trees to bloom earlier and prolong the growth period. By regarding the high vernalization requirement of main olive variety in the study area, a 6°C temperature increase may significantly decrease olive yields and will force farmers to transition to new varieties with relatively low vernalization requirements. According to both RCP scenarios, there is a possibility of extension of suitable areas for olive cultivation towards low to mid-elevation plateaus and mid-elevation slopes of mountainous areas and high plateaus particularly facing suitable aspects to lower negative effects of projected future warming and dryness.

Bu çalışma, aç bırakılmadan kırılmış arpa tüketimine dayalı tüy dökümü uygulamasında, farklı sıcaklık ve canlı ağırlıkların yumurta tavuklarının performansı, yumurta kalitesi, bazı organ ağırlıkları ve bacak kemiği özellikleri üzerindeki etkilerini değerlendirmeyi amaçlamıştır. Çalışmada, zenginleştirilmiş kafeslerde yetiştirilen 80 haftalık yaşta 288 adet kahverengi yumurtacı tavuk (Lohmann Brown-Classic) kullanılmıştır. Tavuklar tüy dökümü öncesi canlı ağırlık (hafif, orta ve ağır) ve sıcaklık (kontrol ve döngüsel sıcaklık) gruplarına ayrılmıştır. Tüy dökümü döneminde, tavuklar kontrol grubunda 21-22°C'de, döngüsel sıcaklık grubunda ise günlük 10:00-18:00 saatleri arasında 32°C'de ve 18:00-10:00 saatleri arasında 21-22°C'de tutulmuştur. Tüy dökümü programı gruplardaki tavukların 35 günde canlı ağırlıklarının %25'ini kaybetmesini sağlamış ve bu dönemde tavuk ölümü olmamıştır. Tavukların tüy dökümü sonrası performansları perdeli bir kümeste üç ay bahar ve bir ay yaz mevsimi koşullarında incelenmiştir. Tüy dökümü periyodundaki döngüsel yüksek sıcaklık uygulaması, verim dönemi performansını etkilememiş, ancak yumurtaların kırılma direnci, Haugh birimi ve ak indeksini iyileştirmiştir. Ayrıca, canlı ağırlığı yüksek tavuklarda, tüy dökümü sonrası dönemde yumurta ağırlığı, tibia ağırlığı, uzunluğu, genişliği, kırılma direnci ile femurun ağırlığını ve kırılma direncini artmıştır. Yumurta verimi, yumurta ağırlığı, yemden yararlanma oranı ve yumurta kalite özellikleri tüy dökümü sonrası dönemde, tüy dökümü öncesine göre önemli ölçüde iyileşmiştir. Tüy dökümü döneminde tavuklarda, dalak ağırlığı artarken, yumurtalık, yumurta kanalı ve karaciğer ağırlıkları önemli ölçüde azalmıştır. Tüy dökümü döneminde uygulanan döngüsel yüksek sıcaklığın ikinci verim döneminde bazı yumurta kalite özelliklerini olumlu etkilediği ortaya çıkmıştır.

Plants need to accumalate heat to complete a particular or whole growth period. This accumalation depends on temperature thresholds above or below which plant growth ceases, and on air temperature. It can be speculated that more heat accumulation is available for plants due to rapid warming within three or four decades. This study presents more recent heat accumulation for plants, quantified using a useful index called growing degree day (GDD), for five locations (Söke, Kuşadası, Aydın, Sultanhisar and Nazilli) in Aydın, Türkiye, during the latest climatic normal period from 1991 to 2020. GDD values were calculated both in monthly basis from March through October, and in daily basis from March 1st to October 31st. Monthly GDD averages, as expected, showed a pattern that increased from March to July or August, then decreased thereafter till October. Range and standart deviation showed approximately an opposite pattern, suggesting higher uncertainity in relatively colder months. The results are expected to provide farmers or agricultural practitioners with the latest averages of GDD to predict plant growth and development.

Seed maturation in hemp is not homogeneous, and the harvest is done when the seeds are 70-80% mature. This study was carried out to determine the germination and emergence performance of hemp (Cannabis sativa L.) seeds at different maturation stages. Mature, semi-mature and immature seeds were used as material in the study. The study was carried out as two separate experiments, germination and emergence. The germination test was carried out in petri dishes and the emergence test in viols. Both experiments were carried out according to the randomized complete block design with 3 replications. Germination rate (%), shoot and root length (mm), shoot and root fresh weight (mg plant-1) and shoot and root dry weight (mg plant-1) parameters were investigated in the germination study. In the emergence study, the emergence rate (%), seedling and root length (mm), seedling and root fresh weight (mg plant-1), and seedling and root dry weight (mg plant-1) values were examined. In the germination study, semi-mature seeds germinated but did not develop. Therefore, no data could be obtained from other parameters except germination rate. In germination study, germination rate varied between 6.67 and 84.67%, shoot length 27.2-38 mm, root length 39.4-50.8 mm, shoot fresh weight 30.66-49.89 mg plant-1, root fresh weight 4.32-7.69 mg plant-1, shoot dry weight 3.25-7.99 mg plant-1, root dry weight 0.68-2.03 mg plant-1. In the emergence study, emergence rate ranged between 5.18-82.69%, shoot length 68-136.4 mm, root length 37.4-69.6 mm, shoot fresh weight 60.33-154.80 mg plant-1, root fresh weight 27.30-46.73 mg plant-1, shoot dry weight 3.97-10.24 mg plant-1, root dry weight 2.61-5.43 mg plant-1. In both experiments, the highest values obtained from all the examined traits were obtained from mature seeds. In both studies, the highest values were obtained from mature seeds for all traits examined. Semi-mature and immature seeds gave similar results in terms of the traits examined.

Promoting rooftop farming using suitable grow bag media is of utmost importance in densely populated cities like Lalitpur to sustain the human race. To assess the productivity and other economic characteristics of bell peppers, an experiment was conducted from Feb 2022 to Jun 2022 in different grow bag media on the premises of Vegetable Crop Development Centre, Khumaltar, Lalitpur. The experiment was conducted in Randomized Complete Block Design (RCBD), with four replications and five treatments with growing media: Coco peat: vermicompost (1:1), Soil: sand: FYM (Farmyard Manure) (1:1:1), Soil: Sand: FYM: Vermicompost (1.25:0.75:0.5:0.5), Soil: vermicompost (1:1), and Soil: ash: FYM (1:0.5:1) on the cultivar California Wonder. Growth parameters like plant height (47.865 cm), number of leaves (50.6), number of branches/plant (9.5), and stem diameter, and yield parameters like total fruit weight (1693.93 gm), average fruit weight (84.68 gm), number of fruits (42.75), fruit diameter (6.37 cm), and fruit length (7.22 cm) were observed during the experiment. The media consisting of T3 (Soil: Sand: FYM: Vermicompost (1.25:0.75:0.5:0.5)) proved to be statistically superior over the rest of the combinations for almost all aspects under investigation, whereas comparatively poor performance was observed in Coco peat: vermicompost (1:1) media. This study suggests that the grow bag media of Soil: Sand: FYM: vermicompost (1.25:0.75:0.5:0.5) gave the highest gross return and net return, with the highest B: C ratio (2.08) observed in the grow bag media of Soil: Sand: FYM (1:1:1).

The current study aimed to evaluate the effect of extraction solvents on the anti-bacterial and anti-fungal efficacy of the stem and roots of Ajuga integrifolia Buch. Ham. ex D. Extracts were prepared in different solvents and tested against fungi and bacteria species including, Agrobacterium tumefaciens, Xanthomonos oryzae, Citrobacter freundi, Alternaria alternata, A. solani and Aspergillus niger. Antibacterial efficacy of the Ajuga integrifolia was carried out by disc diffusion susceptibility method and antifungal efficacy by well diffusion susceptibility method. Methanol stem extract revealed efficacy against C. freundi by producing a 63% zone of inhibition at 3000µg/disc while methanol roots extract produced 77% ZI against A. tumifaciens. Methanol root and stem extracts produced an equal zone of inhibition (97%) at 1000 µg/ml concentration against A. alternata and A. solani. The results of the study clearly stated that the polarity of the solvents used in the extraction procedure affects the bioactivities of the extracts.

The Bangshi River flows through the industrial regions of Dhaka and Tangail, polluted by various wastes, including industrial effluents. This study assessed water quality and heavy metal concentrations in the river, focusing on two fish species, Tengra (Mystus nigriceps) and Tara baim (Macrognathus aral). Fish samples were taken from one station during the wet season, whereas water samples were taken from three stations throughout the dry season (June to August 2022) and wet season (February to May 2022). The parameters analyzed included water temperature, pH, dissolved oxygen (DO), biological oxygen demand (BOD), total dissolved solids (TDS), electrical conductivity (EC), alkalinity, and the concentrations of heavy metals such as lead (Pb), cadmium (Cd), copper (Cu), and manganese (Mn). Water temperature ranged from 30.5 to 32.3°C during the wet season and 19.91 to 20.5°C in the dry season, remaining within acceptable limits. pH values were within the standard range. However, EC exceeded irrigation standards during the dry season (828 to 903 µS/cm). TDS levels were 83 to 175 ppm in the wet season and 328 to 563 ppm in the dry season, with most dry season samples exceeding fish culture limits. DO values (1.98 to 4.26 ppm) were consistently below recommended levels, while BOD (2.33 to 4.83 ppm) and alkalinity (80.7 to 382.23 ppm) surpassed permissible thresholds, particularly in the dry season. Heavy metals in water were generally within safe limits except for Pb (4.40 to 5.002 ppm). In fish, Pb concentrations exceeded permissible levels (5.35 ppm in Tengra, 4.46 ppm in Tara baim), while Cd, Cu, and Mn were within acceptable thresholds. The elevated Pb levels likely stem from effluents released by glass, textile, and paint industries. These findings highlight potential risks to human health from consuming water and fish from the Bangshi River, underscoring the urgent need for improved pollution control strategies.

This research aims to control the quality of cocoa oil industry products through the application of the six sigma method. This research was carried out in the cocoa oil industry centre area in Southeast Sulawesi Province, which accommodates 3 (three) regions, namely; North Kolaka Regency, East Kolaka Regency, and Kolaka Regency. The population in this study were owners or managers of the cocoa oil industry, totalling 130 industries. The research sample used census techniques, namely; the entire population is used as a sample. Data collection techniques were carried out through interviews and direct observation at each industrial location during the cocoa oil production process. The analysis used in this research is Six Sigma analysis with the Define, Measure, Analysis, Improve, and Control (DMAIC) approach. The research results show that there is an average of 17.74% defective cocoa oil products for each production. In addition, the results of this study show that the types of defective cocoa oil products are smell mismatch (17.74%), color (3.71%), taste (7.60%), and water content (70.95%). The results of this research also provide information that the solution needed to control the quality of cocoa oil products is replacing production equipment and production support, establishing partnerships with cocoa bean suppliers from more profitable sources, improving the soft and hard skills of the workforce as well as mentoring, providing machines. more adequate packaging, as well as the use of ethanol solution in the extraction process.

Access to capital for farmers requires the intermediation function of Islamic financing amidst high business risks. The research aims to identify the appropriate construction of Islamic financing products in the agricultural sector to increase access to capital in the agricultural sector in Indonesia. The research method uses the Interpretative Structural Modeling (ISM) Analysis model. The study of Islamic agricultural financing was carried out with 3 structural elements namely purposes, constraints and policy recommendations. The findings of the research results establish 3 policy clusters. First, the construction of a parallel greeting contract model between Islamic Banking, Rice Mills, and Agents in the Warehouse Receipt System scheme. Second, strengthening farmers' access to Islamic financial institutions and adjusting Sharia financial contracts in farming capital at the central and regional levels. Third, Islamic Cooperative microfinance institutions and financial arrangements for Islamic financing in the agricultural sector. This research recommendation provides valuable insights and practical solutions for implementing Islamic financial products with an agribusiness system approach based on feasibility studies of financial arrangements and risk mitigation for agricultural financing.

Arabica coffee (Coffee arabica L.) is among the most popular and widely consumed drinks globally today. The current study aimed to detect and quantify of potential mycotoxins in coffee beans and to investigate their potential risk on HepG-2 human liver cancer cells and male albino rats. Eighteen fully matured coffee beans samples were collected from different geographical locations in Saudi Arabia. Mycotoxins detected and quantified utilizing high-performance liquid chromatography (HPLC). Cytotoxicity of the detected toxins was investigated utilizing the MTT assay method toward HepG-2 cells and male albino rats. Cytotoxic properties of the detected mycotoxins were investigated at doses of 31.25, 62.50, 125, 250 and 500 µg/ml following exposure durations of 24 and 48 h in comparison with control. HPLC analysis revealed the detection of four Alternaria toxins (ALT-Ts) namely Altenuene (ALT), Tenuazonic acid (TA), Alternariol (AOH), and Altenuisol being AOH reported as the frequently detected mycotoxin in 18 coffee beans samples representing 75 % with quantity ranged 0.98 to 8.97 μg/g. OTA was frequently detected (10 samples, 55.5%) in concentrations varied from 0.75 to 9.87 μg/g. In addition, Aflatoxin B1 was also detected with low quantity ranged 0.89 to 3.22 μg/g. Treatment of HepG-2 cell line with combined Alternaria toxins (ALT, TeA, AOH, and Altenuisol), Aflatoxin B1 (AFB1) and Ochratoxin A (OTA) as well as their combination triggered a decline in cell viability that was proportional to the dose administered. The elevated doses (62.50, 125, 250 and 500 µg/ml) notably reduced cell viability and induced cellular damages when contrasted with the lowest dose (31.25 µg/ml) and control. The IC50 for the tested mycotoxins registered for ALT-Ts, AFB1 and OTA as well as their combination were 115.95, 56.68, 59.86 and 31.05 µg/ml after 48 h of exposure. Secreting inflammatory markers (Interleukin-6 (IL-6), interleukin-1β (IL-1β) and COX-2), and oxidative markers (total antioxidant capacity (TAC), glutathione (GSH) and nitrite (NO) were downregulated by all mycotoxins with the highest decrease induced by their mixture. Malonaldehydes (MDA) and TAC were significantly increased by all toxins in treated rats. Hematological results revealed that AFB1, OTA and ALT-Ts-treated rats exhibited a notable decline in hemoglobin (Hb), red blood cells (RBCs), white blood cells (WBCs), and packed cell volume % (PCV). However, single and double dose-treated rats with OTA, Mix1 and Mix2 exhibited a marked increase in WBCs. The results demonstrated a notable decline in liver and kidney functions in all mycotoxins-treated rats. The studied organs (liver, kidney and spleen) exhibited significant damage, with the most severe alterations observed in the Mix1 and Mix2-treated group in comparison with the groups receiving individual treatment. This indicates that the mixture of AFB1, ALT-Ts and OTA had an enhanced toxic consequence against treated HepG-2 cell line and rats. Our results indicated the synergistic effect of the combined different mycotoxins against HepG-2 human liver cancer cells and male albino rats. This work could help fill in data gaps and enhance risk assessment for human health by addressing some of the possible risks associated with Alternaria toxins

The study aimed to test the cellulolytic ability of Trichoderma AA1 and AA2 inoculum as local Indonesian isolates and their application in the fermentation of Gliricidia sepium leaf meal. Measurement of cellulolytic ability includes qualitative cellulase activity (measurement of clear zone formation around fungal colonies) and quantitative cellulase activity (measurement of the amount of reducing sugar produced by broth enzymes from fungal cultures). Experiments of Gliricidia sepium fermentation using Trichoderma AA1 and AA2 were each designed using Completely Randomized Design. The treatments used consisted of fermentation duration of 0 (control), 2, 4, and 6 days—identification of Trichoderma AA1 and AA2 species using molecular identification method. The research results show that qualitative cellulase activities in Trichoderma AA2 were significantly higher compared to Trichoderma AA1. The CZD/CD ratio of Trichoderma AA1 and AA2 were 2.39±0.11 and 1.97±0.10, respectively. The CMC-ase activity of Trichoderma AA2 was 0.332±0.038 µmol/ml/min, and this was significantly higher compared to TrichodermaAA2, which was only 0.201±0.021 µmol/ml/min. Gliricidia sepium fermentation using AA1 and AA2 inoculum showed increased nutritional value, including soluble protein content, In-vitro digestibility of dry matter, and In-vitro digestibility of organic matter in 2 and 4 days of fermentation. There was a decrease in nutritional value in 6 days of fermentation. Trichoderma AA1 and AA2 were identified as Trichoderma koningiopsis and Trichoderma asperellum, respectively. The study concluded that T. Koningiopsis AA1 and T. Asperellum AA2 were proven to have cellulolytic abilities and could be used as inoculum in Gliricidia sepium fermentation to increase its nutritional value.

Water supply which is a resource that must be sufficient in paddy farming. Reservoirs are civil construction buildings in the field of hydrology that are used to hold water so that it does not go straight to the sea, so that it can be used to improve the availability of water supply to paddy fields that cannot be reached by technical irrigation. The purpose of this study was to differentiating between the effects on water availability, farmer productivity, and economic impacts of the construction of the reservoir on the community. This study was conducted in Sanjai Village, East Sinjai District, Sinjai Regency. A total of 55 respondents of this study were farmers whose paddy fields used water from the reservoir and planted paddy as the main commodity of their farming. The method used to analyse the data was descriptive analysis and paired sample mean different test. The results of the study showed that the effect of reservoirs on water availability in addition to increasing farmer productivity can also reduce the risk of agricultural business due to drought and increase opportunities for farming, especially during the dry season. In addition, the construction of the reservoir has affected the availability of water in Sanjai Village, which was experienced by farmers after the construction of the reservoir, as well as a decrease in the intensity of floods and droughts, the smooth supply of water for the needs of farmers in farming in paddy fields so that the availability of water for farming in paddy fields is more sufficient after the construction of the reservoir and the impact after the development of the reservoir, from an economic point of view, the community was increasing production and productivity. It can be concluded that the construction of the reservoir has been achieved and the function of the reservoir for farmers was very useful and can also change cropping patterns as well as improve crop cultivation.

This study analyzes the price fluctuations of cooking oil in the Indonesian consumer and wholesale markets using the Autoregressive Conditional Heteroskedasticity (ARCH) model across January 2018 to January 2024 period that encompasses the period of study. This study covered the period measuring from January 2018 to January 2024. As per conclusions drawn upon the tests for stationarity, the series has become non-stationary after a second difference. An analysis of the results indicates substantial and even large amounts. Consumer price’s ARCH (α) and GARCH (β) coefficients are 0.569707, and the coefficients for wholesale prices are 1.29 and -0.13 respectively. This indicates that there is the presence of persistent volatility (α + β > 0) in the market. In view of these results, it is very clear that certain policy measures need to be put in place. Such measures should include the stabilization of the delivery mechanism through proper stock management and strategic price mechanisms to avoid wide fluctuations in price. Two other factors that may help in price variations stability are increasing market openness and promoting location diversification of supply sources.

From different fields in Mosul city, we collected sixteen samples of soil surrounding the rhizosphere zone to isolate and characterize rhizobacterial species based on biochemical tests, 16S rRNA gene primer amplification using PCR and nucleotide sequence analysis, and similarity to global isolates in the gene bank. All isolates were bacilliform, with clear differences in their response to Gram staining and biochemical tests. On the other hand, the results showed that root colonizing bacteria could produce different amounts of indole-3-acetic acid (IAA). Molecular analysis tests based on the 16S rRNA primer gene were carried out to characterize the isolated bacteria at the molecular level and showed 99% homology with Azotobacter tropicalis SC39, Azotobacter chroococcum A11, Bacillus subtilis N22 and Ralstonia pickettii ULM005, which are registered worldwide in GenBank. It should be noted that in the diagnostic isolate R. pickettii both A and T were deleted, G was replaced by C and T was added at position 508. On the other hand, in the B. subtilis isolate, the nitrogenous bases A, G and G were deleted and the nitrogenous base G was replaced by the base C. The data for the third isolate, A. tropicalis, showed deletion of the nitrogenous bases C and T and replacement of G by A and A by T. In the fourth isolate, A. chroococcum, deletion of the nitrogenous base, replacement of C by A and C by T and addition of G, T and A in three positions were observed. These will be used as the basis for future scientific experiments to develop new biofertilizers from the rhizobacteria studied for the production of environmentally sustainable crops.

Cocoa is one of the main plantation crops in North Kolaka, Southeast Sulawesi, and is a strategic plantation commodity that tends to be important for the national economy because it provides employment, a source of foreign exchange, and a source of income for farmers. Manure is a waste product from livestock manure, one of which is cattle, which can be used to add nutrients and improve the physical and biological properties of soil. Integrated agriculture (crop-livestock integration) is an agricultural system characterized by the interrelationship between crop and livestock components in a farming business or an area. This study was conducted to determine the effect of manure on cocoa crop production, and to find out that feed made from cocoa fruit peel waste can increase the body weight of cattle. To find out how cattle manure and cattle feed using cocoa shell waste can be integrated in increasing cocoa production and increasing body weight in cattle. Based on the results of the research conducted, it was found that the cattle manure treatment showed the best results for each parameter observed in cocoa production, while in the cattle parameter, the provision of additional cocoa peel feed gave the highest results.

Food processing, such as cooking, can affect the bioactive components of the ingredients. In making rendang tempeh, several stages must be gone through, such as curry (gulai) and kalio formation; therefore, the production of rendang tempeh will take time and might be impacted by the changes in nutrients of the tempeh. This research was conducted to observe changes in chemical components, such as proximate components, antioxidants, fatty acids, and isoflavones, during the process of making tempeh rendang. This study processed tempeh into gulai, kalio, and rendang. Chemical analyses such as proximate analysis (water, fat, protein), antioxidant, fatty acid, and isoflavone analysis were carried out at each stage of making the rendang tempeh. Based on the study's results, it was known that the proximate profile increases during the rendang-making process. Linoleic acid, as the main fatty acid content in tempeh, was decreased, as was the isoflavone content. In this study, From the research, it was concluded that the cooking process changes the profile of the bioactive compounds in tempeh, which might be affected by the decrement of phytochemicals. In this study, the degradation pattern of phytochemical compounds, especially isoflavones, remains unknown, as well as whether the compounds are degraded or changed into aglycone forms with higher bioactivity. Therefore, the observation on molecular perspective on nutritional compounds of rendang tempeh during processing from tempe as raw material to rendang tempeh needs to be further conducted to obtain comprehensive information regarding the benefit of transformation of tempe to become a processed food "rendang tempeh."

Adopting biosecurity and vaccination technology is one of the steps that can be taken to overcome and minimize FMD disease in beef cattle farming. This study aims to analyze the farmer's adoption of biosecurity and vaccination technology in the spread of FMD. The research was conducted from November to December 2023 in Bone Regency, South Sulawesi Province. The sample size was 99 farmers whose animals were infected or not infected with FMD. Data collection methods began with observation and interviews using a questionnaire. The data analysis used was qualitative descriptive analysis using a rating scale. Research variables in the adoption process include knowledge, persuasion, decision, implementation, and confirmation. The results showed that the technology adoption process at the knowledge stage was categorized as moderate, the persuasion stage was categorized as high, the decision stage, the implementation stage, and the confirmation stage were categorized as moderate. The average difference from the stages of the technology adoption process of implementing biosecurity and vaccination as a whole is categorized as moderate with a score of 2.15. Keywords: Adoption technology, biosecurity, breeders, disease, vaccination, Bone Regency, farmer adoption.

Declining soil fertility amid the devastating effects of climate change is a threat to the state of food security in developing countries like Kenya. Enhancing production intervention through sustainable farming practices like integrated soil fertility management (ISFM) technologies has been promoted. However, there is a dearth of knowledge revealing the efficacy of such technologies, especially in dry regions of the country. Therefore, this study evaluated the effect of integrated soil fertility management technologies on sorghum productivity in the drier parts of Upper Eastern Kenya. Data was collected from 370 sorghum farmers using a semi-structured questionnaire. The data was analyzed using descriptive statistics (percentages) and Propensity Score Matching (PSM). The initial PSM stage revealed that various socioeconomic and institutional aspects (subsistence farming, access to extension services, perception of innovations, road infrastructure, ISFM awareness and ISFM costs) influence the adoption decision of ISFM. Further, the findings reveal that farmers who adopted ISFM technologies recorded increased sorghum productivity. Hence, the productivity of adopters was better than that of non-adopters. Improving adoption, therefore requires the facilitation of agricultural institutions and service providers who are key in imparting vital knowledge and creating awareness of productive technologies such as ISFM. Similarly, the results have wider policy effects for agriculture, especially in places where ISFM may be very important for long-term food security and agricultural productivity.

Fusarium sacchari is a pathogenic fungus that causes the pokkah boeng disease in sugarcane plants. The symptoms of pokkah boeng disease are systemic, spreading throughout all parts of the plant. The control measures implemented so far still rely on synthetic pesticides but have not yielded optimal results and are not environmentally friendly. One of the environmentally friendly control methods is the use of endophytic fungi. Endophytic fungi live within plant tissues without causing disease symptoms. This research aims to discover endophytic fungi derived from sugarcane and evaluate their ability to suppress the growth of the pathogen F. sacchari. The research began with the isolation of sugarcane tissue, followed by the morphological and molecular characterization of endophytic fungi and testing their inhibitory capacity against the pathogen F. sacchari. Morphological identification was based on colony and microscopic characteristics, while molecular identification was conducted using PCR and sequencing, with similarities based on GenBank. Inhibition tests were conducted through dual culture and volatile compound testing, as well as enzyme production. The research results obtained 38 endophytic fungal isolates from sugarcane plants that have the ability to inhibit the pathogen F. sacchari. Five of these isolates showed the highest inhibition against F. sacchari and produced cellulase, protease, and chitinase enzymes. Molecular identification results indicated that these five-sugarcane endophytic fungal isolates are Daldinia eschscholdzii, Hypoxylon pulicicidum, Trichoderma virens, Trichoderma harzianum, and Diaporthe phaseolorum. The endophytic fungus T. harzianum provides the highest inhibition rate of 83.33% in dual culture and 55.5% inhibition through the production of volatile compounds. Keywords: Endophytic fungi, inhibitory power, Fusarium sacchari, morphological characteristics, molecular characteristics, pokkah boeng disease, sugarcane plants.

Faculty of Economics, Tay Nguyen University, 567 Le Duan Street, Buon Ma Thuot City, Dak Lak Province, Vietnam. The study aims to evaluate the economic performance of Participatory Guarantee Systems (PGS) in certifying organic vegetable farmers in Northern Vietnam. Moreover, this study identifies the differentiation between prosperous and non-prosperous farmers by analyzing critical factors associated with farm management. With a multi-stage cluster sampling technique, the data were collected from three provinces in Northern Vietnam between March and April, 2023. The findings indicate that PGS farming has the potential to be more efficient than conventional farming, mainly due to reduced intermediate costs and access to niche markets. However, PGS farms are more labor-intensive due to their reliance on manual practices. In addition, the selling approaches and technical progress significantly impact economic viability. These findings highlighted the need to develop market access and enhance farmers' confidence in sustainable agricultural practices to advise the competitiveness of PGS farms. Keywords: Determinants, economic performance, participatory guarantee systems, organic farms, labor cost, market access, Vietnam.

Variations in macronutrient fertilization and spacing are two important components in increasing shallot yield. This study aims to determine the effect of fertilization and plant spacing on shallot yield. A split-plot design with three replications was applied. The main plots were plant spacing (D1: 20 x 20 cm, D2: 20 x 15 cm, D3: 20 x 10 cm); subplots were fertilization of TSP, NPK, KNO3, Urea, and ZA with four doses: F1 by 188, 600, 0, 180, 400 kg ha-1; F2 by 75, 225, 80, 57, 300 kg ha-1; F3 by 100, 300, 107, 76, 400 kg ha-1; and F4 by 125, 375, 135, 95, 500 kg ha-1, respectively. Data was analyzed using the Anova (Analysis of Varians) and Duncan tests. The number of leaves was positively correlated with wet bulb weight per clump (R2 = 86.36%), and dry bulb weight per clump (R2 = 72.40%). The optimum N, P, and K dosages to achieve the optimum relative yield were 126.85; 178.06; 95.25 kg ha-1. The D3F3 interaction (plant spacing of 20x10 cm with TSP, NPK, KNO3, Urea, and ZA fertilization by 100, 300, 107, 76, and 400 kg ha-1, respectively) enhanced the dry bulbs weight per plot by 84.50%.