1. Synchrotron microbeam radiation therapy induces hypoxia in intracerebral gliosarcoma but not in the normal brain
- Author
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Emmanuel L. Barbier, Audrey Bouchet, Benjamin Lemasson, Elke Bräuer-Krisch, Nicolas Coquery, Jean A. Laissue, Claire Rome, Céline Le Clec'h, Emmanuel Brun, Raphaël Serduc, Chantal Rémy, Marine Potez, Geraldine LeDuc, Thomas Christen, Anaïck Moisan, INSERM U836, équipe 7, Nanomédecine et cerveau, European Synchrotron Radiation Facility (ESRF)-Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Neuro-imagerie fonctionnelle et métabolique (ANTE-INSERM U836, équipe 5), Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), European Synchrotron Radiation Facility (ESRF), Pathology Institute, University of Bern, Faculty of Physics, Ludwig Maximilians University, INSERM U836, équipe 6, Rayonnement synchrotron et recherche médicale, Conseil Régional Rhône-Alpes, Ligue contre le cancer, comité de la Drôme, Association pour la recherche contre le cancer, Ludwig-Maximilians-Universität München (LMU), Ludwig-Maximilians University [Munich] (LMU), and Serduc, Raphael
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Pathology ,medicine.medical_specialty ,Gliosarcoma ,Synchrotron Microbeam Radiation Therapy ,Brain Tumors ,vessel radiation responses ,X-Ray Therapy ,Tumor vasculature ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,0302 clinical medicine ,Vessel density ,Microbeam radiation therapy ,medicine ,Animals ,Radiology, Nuclear Medicine and imaging ,[SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] ,Glucose Transporter Type 1 ,medicine.diagnostic_test ,Tumor hypoxia ,Brain Neoplasms ,business.industry ,Brain ,Magnetic resonance imaging ,Hematology ,Oxygenation ,9l gliosarcoma ,medicine.disease ,Magnetic Resonance Imaging ,Rats ,3. Good health ,Oxygen ,Oncology ,Oxygen Saturation ,030220 oncology & carcinogenesis ,Rat ,[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] ,Nuclear medicine ,business ,Synchrotrons ,MRI - Abstract
International audience; PURPOSE: Synchrotron microbeam radiation therapy (MRT) is an innovative irradiation modality based on spatial fractionation of a high-dose X-ray beam into lattices of microbeams. The increase in lifespan of brain tumor-bearing rats is associated with vascular damage but the physiological consequences of MRT on blood vessels have not been described. In this manuscript, we evaluate the oxygenation changes induced by MRT in an intracerebral 9L gliosarcoma model. METHODS: Tissue responses to MRT (two orthogonal arrays (2×400Gy)) were studied using magnetic resonance-based measurements of local blood oxygen saturation (MR_SO2) and quantitative immunohistology of RECA-1, Type-IV collagen and GLUT-1, marker of hypoxia. RESULTS: In tumors, MR_SO2 decreased by a factor of 2 in tumor between day 8 and day 45 after MRT. This correlated with tumor vascular remodeling, i.e. decrease in vessel density, increases in half-vessel distances (×5) and GLUT-1 immunoreactivity. Conversely, MRT did not change normal brain MR_SO2, although vessel inter-distances increased slightly. CONCLUSION: We provide new evidence for the differential effect of MRT on tumor vasculature, an effect that leads to tumor hypoxia. As hypothesized formerly, the vasculature of the normal brain exposed to MRT remains sufficiently perfused to prevent any hypoxia.
- Published
- 2013
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