201. Andrographolide ameliorates OVA-induced lung injury in mice by suppressing ROS-mediated NF-κB signaling and NLRP3 inflammasome activation
- Author
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Qiang Xu, Yang Sun, Xiaoling Yang, Shuang Peng, Chunhong Jiang, Wen Liu, Jian Gao, and Wenjie Guo
- Subjects
0301 basic medicine ,Inflammasomes ,Andrographolide ,Anti-Inflammatory Agents ,Pharmacology ,Antioxidants ,NF-κB ,chemistry.chemical_compound ,0302 clinical medicine ,Medicine ,Anti-Asthmatic Agents ,Lung ,NF-kappa B ,Research Paper: Immunology ,ROS ,Inflammasome ,respiratory system ,Oncology ,030220 oncology & carcinogenesis ,Cytokines ,Immunology and Microbiology Section ,Female ,Diterpenes ,Bronchoalveolar Lavage Fluid ,Signal Transduction ,medicine.drug ,Ovalbumin ,Lung injury ,03 medical and health sciences ,Immune system ,NLRP3 ,In vivo ,Immunity ,NLR Family, Pyrin Domain-Containing 3 Protein ,Animals ,Immune response ,Dose-Response Relationship, Drug ,business.industry ,andrographolide ,Pneumonia ,asthma ,In vitro ,respiratory tract diseases ,Mice, Inbred C57BL ,Disease Models, Animal ,Oxidative Stress ,030104 developmental biology ,Gene Expression Regulation ,chemistry ,Immunology ,Reactive Oxygen Species ,business - Abstract
// Shuang Peng 1,* , Jian Gao 1,* , Wen Liu 1 , Chunhong Jiang 2 , Xiaoling Yang 2 , Yang Sun 1 , Wenjie Guo 1 and Qiang Xu 1 1 State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China 2 State Key Laboratory of Innovative Nature Medicine and TCM Injections, Jiangxi Qingfeng Pharmaceutical Co., Ltd., Ganzhou, China * These authors have contributed equally to this work Correspondence to: Qiang Xu, email: // Wenjie Guo, email: // Yang Sun, email: // Keywords : andrographolide; asthma; NF-κB; NLRP3; ROS; Immunology and Microbiology Section; Immune response; Immunity Received : August 15, 2016 Accepted : October 14, 2016 Published : October 26, 2016 Abstract In this study, we attempted to explore the effect and possible mechanism of Andrographolide on OVA-induced asthma. OVA challenge induced significant airway inflammatory cell recruitment and lung histological alterations, which were ameliorated by Andrographolide. The protein levels of cytokines in bron-choalveolar fluid (BALF) and serum were reduced by Andrographolide administration as well as the mRNA levels in lung tissue. Mechanically, Andrographolide markedly hampered the activation of nuclear factor-κB (NF-κB) and NLRP3 inflammasome both in vivo and vitro thus decreased levels of TNF-α and IL-1β. Finally, we confirmed that ROS scavenging was responsible for Andrographolide’s inactivation of NF-κB and NLRP3 inflammasome signaling. Our study here revealed the effect and possible mechanism of Andrographolide on asthma, which may represent a new therapeutic approach for treating this disease.
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- 2016