49 results on '"Alp, Alpaslan"'
Search Results
2. A household survey; contamination related characteristics of COVID-19
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Atılmış, Duygu, primary, Erik, Hanife, additional, Özaltun, Şahin, additional, Dizman, Gülçin, additional, Karahan, Gizem, additional, Önder, İlke, additional, Alp, Alpaslan, additional, and Akın, Levent, additional
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- 2023
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3. Epstein-barr virus-positive leiomyosarcoma in immunocompetent patients
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Al-tarawneh, Hassan, primary, Alp, Alpaslan, additional, Gedikoglu, Gokhan, additional, and Kosemehmetoglu, Kemal, additional
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- 2023
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4. Clinical Validation and Evaluation of a Colorimetric SARS-CoV-2 RT-LAMP Assay Against RT-PCR.
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Erdem, Murat, Andaç-Özketen, Ayşe, Özketen, Ahmet Çağlar, Karahan, Gizem, Tozluyurt, Abdullah, Palaz, Fahreddin, Alp, Alpaslan, and Ünal, Serhat
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- 2023
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5. Sensitivity Affected by Disease Severity and Serum Sampling Time: a Performance Evaluation of Six SARS-CoV-2 Antibody Immunoassays
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Şener, Burçin, primary, Kırbaş, Ekin, additional, Sancak, Banu, additional, Gözalan, Ayşegül, additional, Evren, Ebru, additional, Karahan, Zeynep Ceren, additional, Zeytinoğlu, Ayşın, additional, Dinç, Bedia, additional, Alp, Alpaslan, additional, Dizman, Gülçin Telli, additional, Metan, Gökhan, additional, Birengel, Serhat, additional, Gülten, Ezgi, additional, Taşbakan, Meltem, additional, and Ayhan, Müge, additional
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- 2022
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6. Hacettepe Sağlık Kohortu (HU-CoVaCS): Çalışma Tasarımı, Başlangıç Viziti Değerlendirmesi ve COVID-19 Aşılıların İlk Üç Aylık Takip Verileri.
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Karadoğan, Eda, Uzar, Hanife, Sertçelik, Ahmet, Temizer, Mithat, Kara, Şeyma Aliye, Özyürek, Damla, Has-Akdağ, Fahriye, Özden, Mustafa Enes, Alp, Alpaslan, Pınar, Aslı, Güçiz-Doğan, Bahar, Şener, Burçin, Şahan, Ceyda, Yüce, Deniz, Ayhan-Başer, Duygu, Aksoy, Hilal, Fidancı, İzzet, Özcebe, Lütfiye Hilal, Akova, Murat, and Portakal, Oytun
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IMMUNIZATION ,COVID-19 vaccines ,MEDICAL students ,CASE-control method ,DESCRIPTIVE statistics ,LONGITUDINAL method - Abstract
Copyright of Klimik Journal / Klimik Dergisi is the property of DOC Design & Informatics Co. Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2023
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7. Epidemiologic trends in HBV infections at a reference centre in Turkey: an 11-year retrospective analysis
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Ergunay, Koray, Balaban, Yasemin, Cosgun, Erdal, Alp, Alpaslan, Simsek, Halis, Sener, Burcin, Tatar, Gonca, and Hascelik, Gulsen
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- 2012
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8. MEMS biosensors for detection of methicillin resistant Staphylococcus aureus
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Ceylan Koydemir, Hatice, Külah, Haluk, Özgen, Canan, Alp, Alpaslan, and Hasçelik, Gülşen
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- 2011
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9. Human Ace D/I Polymorphism Could Affect the Clinicobiological Course of COVID-19
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Aladag, Elifcan, primary, Tas, Zahit, additional, Ozdemir, Bilgesu Safak, additional, Akbaba, Tayfun Hilmi, additional, Akpınar, Meltem Gulsun, additional, Goker, Hakan, additional, Unalan-Altintop, Tugce, additional, Inkaya, Ahmet Cagkan, additional, Alp, Alpaslan, additional, Metan, Gokhan, additional, Haznedaroglu, Ibrahim Celalettin, additional, Balci-Peynircioglu, Banu, additional, and Sayinalp, Nilgun, additional
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- 2021
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10. Mapping of serological testing and SARS-CoV-2 seroprevalence studies performed in 20 European countries, March-June 2020
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Bubba, Laura, primary, Simmonds, Peter, additional, Fischer, Thea K, additional, Harvala, Heli, additional, Weseslindtner, Lukas, additional, Spuchhammer-Stockl, Elisabeth, additional, Marijke, Reynders, additional, Dedeic Ljubovic, Amela, additional, Aleksandrova, Dora, additional, Tabain, Irena, additional, Rainetova, Petra, additional, Jirincova, Helena, additional, Bang, Didi, additional, Fickenscher, Helmut, additional, Krumbholz, Andi, additional, Panning, Marcus, additional, Huzly, Daniela, additional, Eis-Hübinger, Anna M, additional, Papa, Anna, additional, Sourvinos, George, additional, Zafiropoulos, Alexandros, additional, Ásgeirsson, Asgeir Erlendur, additional, Connell, Jeff, additional, Percivalle, Elena, additional, Cassaniti, Irene, additional, Karen, Couderé, additional, Lind, Andreas, additional, Nordbo, Svein Arne, additional, de Sao Jose Nascimento, Maria, additional, Mesquita, Joao Rodrigo, additional, Brito, Maria, additional, Guiomar, Raquel, additional, Rodrigues, Ana Paula, additional, Fonseca e Silva, Daniela, additional, Abreu, Ana, additional, Dessa, Paulo, additional, Popescu, Corneliu Petru, additional, Berginc, Natasa, additional, ProsencTrilar, Katarina, additional, Poljak, Mario, additional, Rabella, Nuria, additional, Esperalba, Juliana, additional, Perez-Olmeda, Mayte, additional, Fernández-García, Aurora, additional, Bogdanovic, Gordana, additional, Muschiol, Sandra, additional, Bohn Christiansen, Claus, additional, Can Sarınoglu, Rabia, additional, Karahasan Yagci, Aysegul, additional, Zeytinoglu, Aysin, additional, Soylu, Mehmet, additional, Sener, Burcin, additional, and Alp, Alpaslan, additional
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- 2021
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11. Lack of awareness of Hepatitis B screening and vaccination in high-risk groups
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BALABAN, H. Yasemin, primary, ASLAN, Abdullah Tarık, additional, AYAR, Şefika, additional, DAĞ, Osman, additional, ALP, Alpaslan, additional, ŞİMŞEK, Cem, additional, VAHABOV, Cavanşir, additional, YILDIRIM, Tolga, additional, GÖKER, Hakan, additional, BÜYÜKAŞIK, Yahya, additional, and ŞİMŞEK, Halis, additional
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- 2021
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12. Hepatitis E Virus Prevalence and Associated Risk Factors in High-Risk Groups: A Cross-Sectional Study.
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Balaban, Hatice Yasemin, Aslan, Abdullah Tarık, Akdoğan-Kittana, Fatma Nur, Alp, Alpaslan, Dağ, Osman, Ayar, Şefika Nur, Vahabov, Cavanşir, Şimşek, Cem, Yıldırım, Tolga, Göker, Hakan, Ergünay, Koray, Erdem, Yunus, Büyükaşık, Yahya, and Şimşek, Halis
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- 2022
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13. Outcome of noncritical COVID-19 patients with early hospitalization and early antiviral treatment outside the ICU
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ÇALIK BAŞARAN, Nursel, primary, UYAROĞLU, Oğuz Abdullah, additional, TELLİ DİZMAN, Gülçin, additional, ÖZIŞIK, Lale, additional, ŞAHİN, Taha Koray, additional, TAŞ, Zahit, additional, İNKAYA, Ahmet Çağkan, additional, KARAHAN, Sevilay, additional, ALP, Şehnaz, additional, ALP, Alpaslan, additional, METAN, Gökhan, additional, ZARAKOLU, Pınar, additional, SAİN GÜVEN, Gülay, additional, ÖZ, Şerife Gül, additional, TOPELİ, Arzu, additional, UZUN, Ömrüm, additional, AKOVA, Murat, additional, and ÜNAL, Serhat, additional
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- 2021
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14. Is COVID-19 a risk factor for invasive pulmonary aspergillosis in critically ill patients?
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Er, Berrin, primary, Er, Ahmet Görkem, additional, Metan, Gökhan, additional, Halaçlı, Burçin, additional, Ortaç Ersoy, Ebru, additional, Hazırolan, Gülşen, additional, Alp, Alpaslan, additional, Sarıbaş, Zeynep, additional, Arıkan Akdağlı, Sevtap, additional, Topeli, Arzu, additional, and Uzun, Ömrüm, additional
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- 2021
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15. Pneumococcal carriage in children with COVID-19
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Aykac, Kubra, primary, Ozsurekci, Yasemin, additional, Cura Yayla, Burcu Ceylan, additional, Evren, Kubra, additional, Lacinel Gurlevik, Sibel, additional, Oygar, Pembe Derin, additional, Yucel, Mihriban, additional, Karakoc, Ayse Esra, additional, Alp, Alpaslan, additional, Cengiz, Ali Bulent, additional, and Ceyhan, Mehmet, additional
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- 2021
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16. Evaluation of 2015-2016 MOTAKK HBV DNA and HCV RNA External Quality Assessment National Program Results
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Karatayli, Ersin, Soydemir, Ege, Aksoy, Zeynep Busra, Kizilpinar, Mehtap, Altay Kocak, Aylin, Karatayli, Senem Ceren, Yurdcu, Esra, Yildirim, Umut, Guriz, Haluk, Bozdayi, Gulendam, Yurdaydin, Cihan, Ilhan, Osman, Yildirim, Yasin, Bozdayi, A. Mithat, Oguz, Acelya Yalcintas, Baris, Ahmet, Alp, Alpaslan, Aksozek, Alper, Sayiner, Arzu, Karagul, Aydan, Ordu, Aylin, Istanbullu, Aye, Otlu, Baris, Aridogan, Buket, Aksu, Burak, Buruk, C. Kurtulus, Karahan, Ceren, Guney, Cakir, Toksoz, Devrim, Yildirim, Dilara, Colak, Dilek, Daglar, Duygu Eren, Findik, Duygu, Kas, Elif, Caliskan, Emel, Zeyrek, Fadile Yildiz, Arslan, Fatma, Demir, Feyza, Milletli, Fikriye, Kibar, Filiz, Ozdincer, Furkan, Dundar, Gulnur, Arslan, Hande, Agca, Harun, Aliskan, Hikmet Eda, Guducuoglu, Huseyin, Fidan, Isil, Akyar, Isin, Afsar, Ilhan, Kaleli, Ilknur, Donmez, Ismail, Yanik, Kemalettin, Midilli, Kenan, Cubukcu, Kivanc, Ozdemir, Mehmet, Acar, Melek, Yalinay, Meltem, Kuskucu, Mert Ahmet, Bakici, Mustafa Zahir, Aydin, Neriman, Yilmaz, Neziha, Ceken, Nihan, Ziyade, Nihan, Yilmaz, Nisel, Ozgumus, Osman Birol, Gitmisoglu, Ozlem, Demirgan, Recep, Kesli, Recep, Guckan, Ridvan, Sertoz, Ruchan, Akgun, Sadik, Aksaray, Sebahat, Tezcan, Seda, Kaygusuz, Sedat, Gokahmetoglu, Selma, Mese, Sevim, Bayik, Seyit Ahmet, Akcali, Sinem, Gurcan, Saban, Karsligil, Tekin, Us, Tercan, Ozekinci, Tuncer, Pilgir, Tulin, Aslan, Ugur, Dinc, Ugur, Coskun, Umut Safiye Say, Cetinkol, Yeliz, Keskin, Yusuf, Ayaydin, Zeynep, Toraman, Zulal Asci, [Karatayli, Ersin -- Kizilpinar, Mehtap -- Altay Kocak, Aylin -- Karatayli, Senem Ceren -- Yurdcu, Esra -- Bozdayi, A. Mithat] Ankara Univ, Hepatol Inst, Ankara, Turkey -- [Soydemir, Ege -- Aksoy, Zeynep Busra] Ankara Univ, Biotechnol Inst, Ankara, Turkey -- [Altay Kocak, Aylin] Baskent Univ, Dept Med Microbiol, Fac Med, Ankara, Turkey -- [Yildirim, Umut] Tomurcuk Technol, Cyberpk, Ankara, Turkey -- [Guriz, Haluk] Ankara Univ, Fac Med, Cebeci Cent Lab, Ankara, Turkey -- [Bozdayi, Gulendam] Gazi Univ, Dept Med Microbiol, Fac Med, Ankara, Turkey -- [Yurdaydin, Cihan] Ankara Univ, Div Gastroenterol, Fac Med, Ankara, Turkey -- [Ilhan, Osman] Ankara Univ, Div Hematol, Fac Med, Ankara, Turkey -- [Yildirim, Yasin] Ankara Univ, Therapeut Apheresis Ctr, Div Hematol, Fac Med, Ankara, Turkey -- [Oguz, Acelya Yalcintas] Iontek Lab, Istanbul, Turkey -- [Baris, Ahmet] RTA Lab, Kocaeli, Turkey -- [Alp, Alpaslan] Hacettepe Univ, Hastaneleri Merkez Lab, Ankara, Turkey -- [Aksozek, Alper] Mugla Sitki Kocman Univ EAH, Mikrobiyoloji Lab, Mugla, Turkey -- [Sayiner, Arzu] Dokuz Eylul Univ, Tip Fak, Tibbi Mikrobiyoloji AD, Izmir, Turkey -- [Karagul, Aydan] Antalya Egitim & Arastirma Hastanesi, Antalya, Turkey -- [Ordu, Aylin] Sisli Florence Nightingale Hastanesi, Mol Mikrobiyoloji Lab, Istanbul, Turkey -- [Istanbullu, Aye] Medipol Hastanesi, Istanbul, Turkey -- [Otlu, Baris] Malatya Univ, Tibbi Mikrobiyoloji AD, Malatya, Turkey -- [Aridogan, Buket] Suleyman Demirel Univ, Tibbi Mikrobiyoloji AD, Isparta, Turkey -- [Aksu, Burak] Marmara Univ, Tip Fak, Tibbi Mikrobiyoloji AD, Istanbul, Turkey -- [Buruk, C. Kurtulus] KATU, Tip Fak, Tibbi Mikrobiyoloji AD, Trabzon, Turkey -- [Karahan, Ceren] Ankara Univ, Tip Fak, Ibn I Sina Hastanesi Merkez Mikrobiyoloji Lab, Ankara, Turkey -- [Guney, Cakir] Ozel SYNLAB Merkezi Lab, Ankara, Turkey -- [Toksoz, Devrim] Referans Klin Lab, Istanbul, Turkey -- [Yildirim, Dilara] Sivas Numune Hastanesi, Mikrobiyoloji Lab, Sivas, Turkey -- [Colak, Dilek] Akdeniz Univ Hastanesi, Merkez Lab, Antalya, Turkey -- [Daglar, Duygu Eren] Aydin Devlet Hastanesi, Tibbi Mikrobiyoloji Lab, Aydin, Turkey -- [Findik, Duygu -- Aslan, Ugur] Selcuk Univ, Tibbi Mikrobiyoloji Lab, Tip Fak, Konya, Turkey -- [Kas, Elif] Ankara 2 Bolge KHB Genel Sekreterligi, Ankara, Turkey -- [Caliskan, Emel] Duzce Univ, Tip Fak, Tibbi Mikrobiyoloji AD, Duzce, Turkey -- [Zeyrek, Fadile Yildiz] Harran Univ, Tip Fak, Tibbi Mikrobiyoloji AD, Sanliurfa, Turkey -- [Arslan, Fatma] Kayseri Egitim & Arastirma Hastanesi, Kayseri, Turkey -- [Demir, Feyza] SB SBU Van EAH Mikrobiyoloji Bolumu, Van, Turkey -- [Milletli, Fikriye] Ahi Evran Univ, EAH Mikrobiyoloji Lab, Kirsehir, Turkey -- [Kibar, Filiz] Cukurova Univ, Tip Fak, Tibbi Mikrobiyoloji AD, Adana, Turkey -- [Ozdincer, Furkan] Gelisim Tip Lab, Istanbul, Turkey -- [Dundar, Gulnur] Ctr Lab Mol Mikrobiyoloji, Istanbul, Turkey -- [Arslan, Hande] Baskent Univ, Enfeksiyon Hastaliklari & Klin Mikrobiyoloji AD, Ankara, Turkey -- [Agca, Harun] Uludag Univ, Tip Fak, Mikrobiyoloji AD, Bursa, Turkey -- [Aliskan, Hikmet Eda] Baskent Univ, Tibbi Mikrobiyoloji AD, Adana Uygulama & Arastirma Merkezi Mikrobiyoloji, Adana, Turkey -- [Guducuoglu, Huseyin] Yuzuncu Yil Univ, Tip Fak, Tibbi Mikrobiyoloji AD, Van, Turkey -- [Fidan, Isil] Gazi Univ, Tip Fak, Tibbs Mikrobiyoloji AD, Ankara, Turkey -- [Akyar, Isin] Acibadem Labmed Mikrobiyoloji Lab, Istanbul, Turkey -- [Afsar, Ilhan] IKCU Ataturk EAH Tibbi Mikrobiyoloji Lab, Izmir, Turkey -- [Kaleli, Ilknur] Pamukkale Univ, Tibbi Mikrobiyoloji Merkez Lab, Denizli, Turkey -- [Donmez, Ismail] Usak Devlet Hastanesi Mikrobiyoloji Lab, Usak, Turkey -- [Yanik, Kemalettin] Ondokuz Mayis Univ, Tip Fak, Tibbi Mikrobiyoloji AD, Samsun, Turkey -- [Midilli, Kenan -- Kuskucu, Mert Ahmet] Istanbul Univ, Cerrahpa Tip Fak, Tibbi Mikrobiyoloji AD, Istanbul, Turkey -- [Cubukcu, Kivanc] Trabzon Kanuni EAH Klin Mikrobiyoloji Lab, Trabzon, Turkey -- [Ozdemir, Mehmet] Necmettin Erbakan Univ Hastanesi, Tibbi Mikrobiyoloji AD, Konya, Turkey -- [Acar, Melek] Samsun Egitim & Arastirma Hastanesi, Mikrobiyoloji Lab, Samsun, Turkey -- [Yalinay, Meltem] Gazi Univ, Tip Fak, Tibbi Mikrobiyoloji AD, Ankara, Turkey -- [Bakici, Mustafa Zahir] Cumhuriyet Univ, Uygulama & Arastirma Hastanesi, Mikrobiyoloji Lab, Sivas, Turkey -- [Aydin, Neriman] Adnan Menderes Univ, Tip Fak, Tibbi Mikrobiyoloji AD, Aydin, Turkey -- [Yilmaz, Neziha] Bozok Univ, Mikrobiyoloji AD, Yozgat, Turkey -- [Ceken, Nihan] Balikesir Devlet Hastanesi, Mikrobiyoloji Lab, Balikesir, Turkey -- [Ziyade, Nihan] Istanbul Adli Tip Kurumu Baskanligi Postmortem Mi, Istanbul, Turkey -- [Yilmaz, Nisel] Tepecik Egitim & Arastirma Hastanesi, Tibbi Mikrobiyoloji Lab, Izmir, Turkey -- [Ozgumus, Osman Birol] Recep Tayyip Erdogan Univ, Tip Fak, Tibbi Mikrobiyoloji AD, Rize, Turkey -- [Gitmisoglu, Ozlem] Necip Fazil Sehir Hastanesi, Mikrobiyoloji Lab, Kahramanmaras, Turkey -- [Demirgan, Recep] Anatolia Genet Lab, Istanbul, Turkey -- [Kesli, Recep] Afyon Kocatepe Univ, Tibbi Mikrobiyoloji AD, Afyon, Turkey -- [Guckan, Ridvan] Amasya Univ, Sabuncuoglu Serefeddin EAH, Mikrobiyoloji Klin, Amasya, Turkey -- [Sertoz, Ruchan] Ege Univ, Tip Fak, Tibbi Mikrobiyoloji AD, Izmir, Turkey -- [Akgun, Sadik] Adiyaman Univ, EAH Tibbi Mikrobiyoloji Lab, Adiyaman, Turkey -- [Aksaray, Sebahat] Haydarpasa Numune Hastanesi, Tibbi Mikrobiyoloji Lab, Istanbul, Turkey -- [Tezcan, Seda] Mersin Univ, Tip Fak, Tibbi Mikrobiyoloji AD, Mersin, Turkey -- [Kaygusuz, Sedat] Kirikkale Univ, Enfeksiyon Hastaliklan & Klin Mikrobiyoloji AD, Kirikkale, Turkey -- [Gokahmetoglu, Selma] Erciyes Univ, Tip Fak, Mikrobiyoloji AD, Kayseri, Turkey -- [Mese, Sevim] Istanbul Univ, Istanbul Tip Fak, Tibbi Mikrobiyoloji AD, Istanbul, Turkey -- [Bayik, Seyit Ahmet] Adana Numune EAH, Tibbi Mikrobiyoloji Lab, Adana, Turkey -- [Akcali, Sinem] Celal Bayar Univ, Tip Fak, Tibbi Mikrobiyoloji AD, Manisa, Turkey -- [Gurcan, Saban] Trakya Univ, Tip Fak, Tibbi Mikrobiyoloji AD, Tekirdag, Turkey -- [Karsligil, Tekin] Gaziantep Univ, Tibbi Mikrobiyoloji AD, Gaziantep, Turkey -- [Us, Tercan] Eskisehir Osmangazi Univ, Tibbi Mikrobiyoloji AD, Eskisehir, Turkey -- [Ozekinci, Tuncer] Dicle Univ, Tip Fak, Tibbi Mikrobiyoloji AD, Diyarbakir, Turkey -- [Pilgir, Tulin] Viromed Lab, Ankara, Turkey -- [Dinc, Ugur] Corlu Devlet Hastanesi, Mikrobiyoloji Lab, Corlu, Turkey -- [Coskun, Umut Safiye Say] TOKAT GOP Univ Hastanesi, Mikrobiyoloji Lab, Tokat, Turkey -- [Cetinkol, Yeliz] Ordu Univ EAH, Tibbi Mikrobiyoloji Lab, Ordu, Turkey -- [Keskin, Yusuf] Cukurova Devlet Hastanesi, Mikrobiyoloji Lab, Adana, Turkey -- [Ayaydin, Zeynep] Gazi Yasargil EAH, Mikrobiyoloji Lab, Diyarbakir, Turkey -- [Toraman, Zulal Asci] Firat Univ, Tip Fak, Tibbi Mikrobiyoloji AD, Elazig, Turkey, Sayiner, Ayca -- 0000-0001-6750-2353, Yurdcu, Esra -- 0000-0002-1441-6408, Kibar, Filiz -- 0000-0003-2983-2399, Otlu, Baris -- 0000-0002-6220-0521, BOZDAYI, ABDURRAHMAN MITHAT -- 0000-0002-2785-1804, Ondokuz Mayıs Üniversitesi, Çukurova Üniversitesi, Tıp Fakültesi, Kırşehir Ahi Evran Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri, Tıbbi Mikrobiyoloji ABD, İç Hastalıkları, Ege Üniversitesi, and Kırıkkale Üniversitesi
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Quality Control ,Microbiology (medical) ,Hepatitis B virus ,medicine.medical_specialty ,Turkey ,Standardization ,HCV RNA ,Hepacivirus ,Microbiology ,Molecular microbiology ,CMV Negative ,External quality assessment ,Proficiency testing ,Humans ,Medicine ,Medical physics ,Hepatitis-B ,General Immunology and Microbiology ,Clinical Laboratory Techniques ,business.industry ,Tests ,Quality control ,Hepatitis B ,Hepatitis C ,external quality control ,viral load ,Clinical Microbiology ,Infectious Diseases ,HBV DNA ,DNA, Viral ,HIV-1 ,Conformity assessment ,Proficiency ,RNA, Viral ,Real-Time Pcr ,business ,Viral load - Abstract
WOS: 000454987700003, PubMed ID: 30522421, MOTAKK, as a national external quality control program has been launched to evaluate the molecular detection of viral infections including HBV DNA and HCV RNA in molecular microbiology diagnostic laboratories in Turkey. This program is prepared in compliance with ISO 17043:2010 (Conformity assessment general requirements for proficiency testing) standards, and aims to take the place of external quality control programs from abroad, contributing to standardization and accuracy of molecular diagnostic tests in our country. The aim of this study was to evaluate 2015 and 2016 results of the MOTAKK External Quality Control Program for HBV DNA and HCV RNA viral load. The calls were announced on the web page of MOTAKK (www.motakk.org). The quality control samples were sent to participating laboratories in 2015 and 2016. Main stocks were prepared from patients with chronic hepatitis B and C who had viral load detection with reference methods according to WHO reference materials for viral load studies to improve quality control sera. From these main stocks, samples with different viral loads were prepared from dilutions of plasma with HBV, HCV, HAV, HIV, Parvovirus B19 and CMV negative serologic markers. Quality control samples were sent to the participating laboratories along with the negative samples in the cold chain. The laboratories accomplished the related tests within 2-3 weeks and entered their results on the MOTAKK web page. These results were analysed according to ISO 13528 (Statistical methods for use in proficiency testing by interlaboratory comparison) and scoring reports were created by a software developed by MOTAKK and sent to participating labs. Each laboratory evaluated their own results in comparison with the other laboratory results, reassessed the tests via observing the distance from the mean result and the reference values. The number of laboratories participating in the HBV DNA and HCV RNA external quality control program was 70-73 in 2015-2016. Participants were able to comply with the program tools, registering, entering results and receiving the results reports problem. In HBV panel, 72.6-89.1% and 84.7-90.3% of the participant laboratories were in 1 standard deviation (SD) in 2015-2016, respectively. In HCV panel, 70.8-89.1% and 84.7-90.3% of the participant laboratories were in 1 SD in 2015-2016, respectively. A national external quality control program for HBV DNA and HCV RNA in Turkey has been prepared for the first time with this project and implemented successfully. All the data provided in the MOTAKK external quality control program final report, compensate all the data provided by the quality control program final reports from abroad; additionally, the report allows comparison of used technologies and commercial products.
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- 2018
17. A COVID-19 first evaluation clinic at a university hospital in Turkey.
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TELLİ DİZMAN, Gülçin, METAN, Gökhan, AYAZ CEYLAN, Çağlayan Merve, ALTUNAY, Hayriye, UZUN, Mertcan, GÜRSOY, Gamze, TAŞ, Zahit, KARAHAN, Gizem, AHMADOVA, Farida, SARICAOĞLU, Tuğba, Cansu ÇALIŞKAN, Zeynep, ALP, Alpaslan, Çağla SÖNMEZER, Meliha, Çağkan İNKAYA, Ahmet, Görkem ER, Ahmet, ALP, Şehnaz ÖZYAVUZ, Abdullah UYAROĞLU, Oğuz, DURUSU TANRIÖVER, Mine, ÇALIK BAŞARAN, Nursel, and DURHAN, Gamze
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SARS-CoV-2 ,COVID-19 ,UNIVERSITY hospitals ,FERRITIN ,MEDICAL personnel ,CLINICS ,COVID-19 pandemic ,VIRUS diseases - Abstract
Background/aim: We aimed to analyze the usefulness of such a reserved area for the admission of the patients’ symptoms suggesting COVID-19 and compare the demographic and clinical characteristics of the patients with COVID-19 and without COVID-19 who were admitted to C1 during the first month of the COVID-19 outbreak in our hospital. Materials and methods: A new area was set up in Hacettepe University Adult Hospital to limit the contact of COVID-19 suspicious patients with other patients, which was named as COVID-19 First Evaluation Outpatient Clinic (C1). C1 had eight isolation rooms and two sampling rooms for SARS-CoV-2 polymerase-chain-reaction (PCR). All rooms were negative-pressurized. Patients who had symptoms that were compatible with COVID-19 were referred to C1 from pretriage areas. All staff received training for the appropriate use of personal protective equipment and were visited daily by the Infection Prevention and Control team. Results: One hundred and ninety-eight (29.4%) of 673 patients who were admitted to C1were diagnosed with COVID-19 between March 20, 2020, and April 19, 2020. SARS-CoV-2 PCR was positive in 142 out of 673 patients. Chest computerized tomography (CT) was performed in 421 patients and COVID-19 was diagnosed in 56 of them based on CT findings despite negative PCR. Four hundred and ninety-three patients were tested for other viral and bacterial infections with multiplex real-time reverse-transcriptase PCR (RTPCR). Blood tests that included complete blood count, renal and liver functions, d-dimer levels, ferritin, C- reactive protein, and procalcitonin were performed in 593 patients. Only one out of 44 healthcare workers who worked at C1 was infected by SARS-CoV-2. Conclusion: A well-planned outpatient care area and teamwork including internal medicine, microbiology, and radiology specialists under the supervision of infectious diseases specialists allowed adequate management of the mild-to-moderate patients with suspicion of COVID-19. [ABSTRACT FROM AUTHOR]
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- 2022
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18. Helicobacter pylori Tanısında Kültür, Real-Time-PCR, Elisa ve Histopatolojik İnceleme Yöntemlerinin Karşılaştırılması
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Maçin, Salih, Alp, Alpaslan, Şener, Burçin, Sökmensüer, Cenk, Orhan, Diclehan, Özen, Hasan, Kav, Taylan, and Selçuk Üniversitesi
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Tıbbi Araştırmalar Deneysel ,Genel ve Dahili Tıp - Abstract
Introduction: There are several methods used for the diagnosis of Helicobacterpylori infections, and there is an increasing demand for theuse of non-invasive, more rapid tests. The aim of the present study wasto compare different diagnostic methods.Methods: A total of 87 patients who had undergone esophagogastroduodenoscopywere included in the study. Biopsy samples obtainedfrom these patients were used for culture, real-time polymerase chainreaction (RT-PCR), and histopathological examination. Stool sampleswere also collected from these patients and were tested using the Helicobacterpylori stool antigen (HpSA) kit. Histopathological examinationwas accepted as the gold standard test.Results: H. pylori was identified by histological examination in 77/87(87.5%) patients, whereas it was negative in 10/87 (12.5%) patients. Furthermore,positive results were obtained in 55 (63.2%), 71 (81.6%), and 77(87.5%) patients using the culture method, HpSA analysis, and RT-PCR method,respectively. The sensitivity and specificity of culture, HpSA, and PCRtests were determined as 71.4% and 100%, 87% and 60%, and 97.4% and80%, respectively. Antibiotic susceptibility tests were performed on 48 outof the 55 culture positive samples. Resistance to clarithromycin was foundin 28 (58.3%), metronidazole in 14 (29.2%), and levofloxacin in 4 (8.3%) ofthe isolates. Resistance to amoxicillin and tetracycline was not observed.Conclusion: There are currently several invasive and non-invasive diagnostictests for the detection of H. pylori infections. Each test has someadvantages and disadvantages. The diagnostic method of choice shouldbe easy and applicable to all age groups., Amaç: Helicobacter pylori enfeksiyonlarının tanısında çeşitli yöntemlerkullanılmaktadır. Invazif olmayan, daha hızlı testlerin kullanımıiçin artan bir talep vardır. Bu çalışmanın amacı farklı tanı yöntemlerinikarşılaştırmaktır.Yöntemler: Çalışma özofagogastroduodenoskopi uygulanan 87 hastayıkapsamaktadır. Bu hastalardan alınan biyopsi örneklerindenkültür, Real-time PCR ve histopatolojik inceleme yapıldı. Ayrıca tümhastalardan dışkı örnekleri de alınıp H.pylori dışkı antijen testi (HpSA)uygulandı. Histopatolojik inceleme altın standart test olarak kabuledildi.Bulgular: Histopatolojik inceleme sonucu; 87 hastanın 77’si H. pyloripozitif (%87.5), 10’u ise (%12.5) negatif olarak belirlenmiştir. Kültür,HpSA ve Real-time PCR’nin duyarlılık ve özgüllükleri sırasıyla %71.4 ve%100, %87 ve %60, %97.4 ve % 80 olarak saptanmıştır. Kültür yöntemiylepozitif saptanan 55 örneğin 48’inde antibiyotik duyarlılık testleriçalışılmıştır. Klaritromisin direnci 28 (% 58.3), metronidazol direnci14 (% 29.2) ve levofloksasin direnci 4 (% 8.3) izolatta saptandı. Hiçbirizolatta amoksisilin ve tetrasiklin direnci saptanmadı.Sonuç: Günümüzde, Helicobacter pylori enfeksiyonlarının tanısındainvazif olan ve invazif olmayan çeşitli tanı testleri kullanılmaktadır.Her testin bazı avantajları ve dezavantajları vardır. Seçilen tanı yöntemi,tüm yaş gruplarına kolaylıkla uygulanabilir olmalıdır.
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- 2018
19. Evaluation of 2015-2016 MOTAKK HBV DNA and HCV RNA external quality assessment national program results
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Karataylı, Ersin, Soydemir, Ege, Aksoy, Zeynep Büşra, Kızılpınar, Mehtap, Altay Koçak, Aylin, Karataylı, Senem Ceren, Yurdcu, Esra, Yıldırım, Umut, Güriz, Haluk, Bozdayı, Gülendam, Yurdaydın, Cihan, İlhan, Osman, Yıldırım, Yasin, Bozdayı, A. Mithat, Yalçıntaş Oğuz, Açelya, Barış, Ahmet, Alp, Alpaslan, Aksözek, Alper, Sayıner, Arzu, Karagül, Aydan, Ordu, Aylin, İstanbullu, Ayşe, Otlu, Barış, Arıdoğan, Buket, Aksu, Burak, Buruk, C. Kurtuluş, Karahan, Ceren, Güney, Çakır, Toksöz, Devrim, Yıldırım, Dilara, Çolak, Dilek, Eren Dağlar, Duygu, Fındık, Duygu, Kaş, Elif, Çalışkan, Emel, Zeyrek, Fadile Yıldız, Arslan, Fatma, Demir, Feyza, Milletli, Fikriye, Kibar, Filiz, Özdinçer, Furkan, Dündar, Gülnur, Arslan, Hande, Ağca, Harun, Alışkan, Hikmet Eda, Güdücüoğlu, Hüseyin, Fidan, Işıl, Akyar, Işın, Afşar, İlhan, Kaleli, İlknur, Dönmez, İsmail, Yanık, Kemalettin, Midilli, Kenan, Çubukçu, Kıvanç, Özdemir, Mehmet, Acar, Melek, Yalınay, Meltem, Kuşkucu, Mert Ahmet, Bakıcı, Mustafa Zahir, Aydın, Neriman, Yılmaz, Neziha, Çeken, Nihan, Ziyade, Nihan, Yılmaz, Nisel, Özgümüş, Osman Birol, Gitmişoğlu, Özlem, Demirgan, Recep, Keşli, Recep, Güçkan, Rıdvan, Sertoz, Ruchan, Akgün, Sadık, Aksaray, Sebahat, Bayık, Seyit Ahmet, Akçalı, Sinem, Gürcan, Şaban, Karslıgil, Tekin, Us, Tercan, Özekinci, Tuncer, Pılgır, Tülin, Aslan, Uğur, Dinç, Uğur, Say Coşkun, Umut Safiye, Çetinkol, Yeliz, Keskin, Yusuf, Ayaydın, Zeynep, and Aşçı Toraman, Zulal
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Viral Yük ,HBV DNA ,HCV RNA ,Dış Kalite Kontrol ,External Quality Control ,Viral Load - Abstract
Ülkemizde, moleküler mikrobiyoloji tanı laboratuvarlarında yapılan HBV DNA ve HCV RNA viral yük saptama testlerinin ulusal bir dış kalite kontrol programında değerlendirilmesi amacıyla MOTAKK (Moleküler Tanıda Kalite Kontrol) Ulusal Programı başlatılmıştır. ISO 17043:2010 (Uygunluk değerlendirmesi- Yeterlilik deneyi için genel şartlar) standartlarına uyularak hazırlanan bu program, ülkemizde yapılan moleküler tanı testlerinin, daha standart ve doğru yapılmasına katkıda bulunarak, yurt dışından sağlanan dış kalite kontrol (DKK) programlarının yerini almayı amaçlamaktadır. Bu çalışmada, MOTAKK DKK Programı kapsamındaki HBV DNA ve HCV RNA viral yük 2015 ve 2016 sonuçlarının değerlendirilmesi amaçlanmıştır. Yapılan çağrılar MOTAKK web sayfası (www.motakk.org) üzerinden ilan edilmiştir. Web sayfası üzerinden kayıt olan katılımcı laboratuvarlara, 2015 ve 2016 yıllarında birer kalite kontrol paneli gönderilmiştir. Çevrimlerde kullanılan paneller, HBV, HCV, HIV, HAV, Parvovirüs B19 ve CMV serolojik belirteçleri negatif olan plazma ile dilüsyon yapılan değişik viral yüklere sahip örnekler ile hazırlanmış, negatif örneklerle beraber soğuk zincirde katılımcı laboratuvarlara ulaştırılmıştır. Laboratuvarlar ilgili testleri 2-3 hafta içerisinde sonuçlandırarak, MOTAKK web sayfasına sonuçlarını girmiştir. MOTAKK tarafından geliştirilen bir yazılım ile katılımcı laboratuvarların sonuçları ISO 13528’e uygun olarak analiz edilerek sonuç raporları oluşturulmuş ve web sayfasına yüklenerek katılımcılara iletilmiştir. Katılımcılar, çalışma sonucunu diğer laboratuvar sonuçları ile karşılaştırmalı olarak değerlendirme imkanına sahip olmuş ve referans değerlere ve ortalama sonuçla ilgili farklılıkları görerek kullandıkları testleri tekrar değerlendirmiştir. HBV DNA ve HCV RNA DKK programına, 2015-2016 yıllarında 70-73 laboratuvar katılmıştır. Katılımcılar, program araçlarına yüksek uyum göstererek kayıt, sonuç girme ve sonuç raporlarının alınmasını aksaksız olarak gerçekleştirmişlerdir. HBV panelinde; 2015 yılında katılımcı laboratuvarların %72.6-89.1’inin, 2016 yılında ise %84.7-90.3’ünün 1 standart sapma (SS) aralığında yer aldığı görülmüştür. HCV panelinde ise; katılımcıların %70.8-89.1’i 1 SS’de, ikinci çağrının yapıldığı 2016 yılında ise, %84.7-90.3’ünün 1 SS’nin içerisinde yer aldığı görülmüştür. Bu proje ile Türkiye’de HBV DNA ve HCV RNA ile ilgili ilk kez ulusal bir DKK programı hazırlanmış ve başarıyla uygulanmıştır. MOTAKK, DKK programı sonuç raporlarında sağlanan bilgiler, yurt dışından sağlanan kalite kontrol programı sonuç raporlarının sağladığı tüm bilgileri karşılamakta; ek olarak kullanılan teknolojilerin ve ticari ürünlerin sağlıklı karşılaştırmalarına olanak sağlamaktadır. MOTAKK, as a national external quality control program has been launched to evaluate the molecular detection of viral infections including HBV DNA and HCV RNA in molecular microbiology diagnostic laboratories in Turkey. This program is prepared in compliance with ISO 17043:2010 (Conformity assessment general requirements for proficiency testing) standards, and aims to take the place of external quality control programs from abroad, contributing to standardization and accuracy of molecular diagnostic tests in our country. The aim of this study was to evaluate 2015 and 2016 results of the MOTAKK External Quality Control Program for HBV DNA and HCV RNA viral load . The calls were announced on the web page of MOTAKK (www.motakk.org). The quality control samples were sent to participating laboratories in 2015 and 2016. Main stocks were prepared from patients with chronic hepatitis B and C who had viral load detection with reference methods according to WHO reference materials for viral load studies to improve quality control sera. From these main stocks, samples with different viral loads were prepared from dilutions of plasma with HBV, HCV, HAV, HIV, Parvovirus B19 and CMV negative serologic markers. Quality control samples were sent to the participating laboratories along with the negative samples in the cold chain. The laboratories accomplished the related tests within 2-3 weeks and entered their results on the MOTAKK web page. These results were analysed according to ISO 13528 (Statistical methods for use in proficiency testing by interlaboratory comparison) and scoring reports were created by a software developed by MOTAKK and sent to participating labs. Each laboratory evaluated their own results in comparison with the other laboratory results, reassessed the tests via observing the distance from the mean result and the reference values. The number of laboratories participating in the HBV DNA and HCV RNA external quality control program was 70-73 in 2015-2016. Participants were able to comply with the program tools, registering, entering results and receiving the results reports without problem. In HBV panel, 72.6-89.1% and 84.7-90.3% of the participant laboratories were in 1 standard deviation (SD) in 2015-2016, respectively. In HCV panel, 70.8-89.1% and 84.7-90.3% of the participant laboratories were in 1 SD in 2015-2016, respectively. A national external quality control program for HBV DNA and HCV RNA in Turkey has been prepared for the first time with this project and implemented successfully. All the data provided in the MOTAKK external quality control program final report, compensate all the data provided by the quality control program final reports from abroad; additionally, the report allows comparison of used technologies and commercial products.
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- 2018
20. Analysis of Fusobacterium nucleatum and Streptococcus gallolyticus in saliva of colorectal cancer patients
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Guven, Deniz Can, primary, Dizdar, Omer, additional, Alp, Alpaslan, additional, Akdoğan Kittana, Fatma Nur, additional, Karakoc, Derya, additional, Hamaloglu, Erhan, additional, Lacin, Sahin, additional, Karakas, Yusuf, additional, Kilickap, Saadettin, additional, Hayran, Mutlu, additional, and Yalcin, Suayib, additional
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- 2019
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21. Genotyping of Mycobacterium tuberculosis clinical isolates in two cities of Turkey: Description of a new family of genotypes that is phylogeographically specific for Asia Minor
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Durmaz Riza, Alp Alpaslan, Saribas Zeynep, Gunal Selami, Allix Caroline, Zozio Thierry, Fauville-Dufaux Maryse, Rastogi Nalin, and Sola Christophe
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Microbiology ,QR1-502 - Abstract
Abstract Background Population-based bacterial genetics using repeated DNA loci is an efficient approach to study the biodiversity and phylogeographical structure of human pathogens, such as Mycobacterium tuberculosis, the agent of tuberculosis. Indeed large genetic diversity databases are available for this pathogen and are regularly updated. No population-based polymorphism data were yet available for M. tuberculosis in Turkey, at the crossroads of Eurasia. Results A total of 245 DNAs from Mycobacterium tuberculosis clinical isolates from tuberculosis patients residing in Turkey (Malatya n = 147 or Ankara n = 98) were genotyped by spoligotyping, a high-throughput genotyping method based on the polymorphism of the Direct Repeat locus. Thirty-three spoligotyping-defined clusters including 206 patients and 39 unique patterns were found. The ST41 cluster, as designated according to the international SpolDB3 database project, represented one fourth and when gathered to three genotypes, ST53, ST50 and ST284, one half of all the isolates. Out of 34 clinical isolates harboring ST41 which were further genotyped by IS6110 and by MIRU-VNTR typing, a typical 2-copy IS6110-RFLP pattern and a "215125113322" MIRU-VNTR pattern were observed among 21 clinical isolates. Further search in various databases confirms the likely Turkish-phylogeographical specificity of this clonal complex. Conclusion We described a new phylogeographically-specific clone of M. tuberculosis, designated LAM7-TUR. Further investigations to assess its frequency within all regions of Turkey and its phylogeographical origin and phylogenetic position within the global M. tuberculosis phylogenetic tree will shed new light on its endemicity in Asia Minor.
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- 2005
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22. Out-patient management of patients with COVID-19 on home isolation.
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Ayaz, Caglayan Merve, Dizman, Gulcin Telli, Metan, Gokhan, Alp, Alpaslan, and Unal, Serhat
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- 2020
23. Prevalence and seasonal distribution of viral etiology of respiratory tract infections in inpatients and outpatients of the pediatric population: 10 year follow-up
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Aykaç, Kübra, primary, Karadağ-Öncel, Eda, additional, Bayhan, Cihangül, additional, Tanır-Başaranoğlu, Sevgen, additional, Akın, Mustafa Şenol, additional, Özsürekci, Yasemin, additional, Alp, Alpaslan, additional, Cengiz, Ali Bülent, additional, Kara, Ateş, additional, and Ceyhan, Mehmet, additional
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- 2018
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24. In vitro and in vivo fitness costs associated with Mycobacterium tuberculosis RpoB mutation H526D
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Rifat, Dalin, primary, Campodónico, Victoria L, additional, Tao, Jing, additional, Miller, James A, additional, Alp, Alpaslan, additional, Yao, Yufeng, additional, and Karakousis, Petros C, additional
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- 2017
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25. Multicenter evaluation of crystal violet decolorization assay (CVDA) for rapid detection of isoniazid and rifampicin resistance in Mycobacterium tuberculosis
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Coban, Ahmet Yilmaz, primary, Akbal, Ahmet Ugur, additional, Bicmen, Can, additional, Albay, Ali, additional, Sig, Ali Korhan, additional, Uzun, Meltem, additional, Selale, Deniz Sertel, additional, Ozkutuk, Nuri, additional, Surucuoglu, Suheyla, additional, Albayrak, Nurhan, additional, Ucarman, Nilay, additional, Ozkutuk, Aydan, additional, Esen, Nuran, additional, Ceyhan, Ismail, additional, Ozyurt, Mustafa, additional, Bektore, Bayhan, additional, Aslan, Gonul, additional, Delialioğlu, Nuran, additional, and Alp, Alpaslan, additional
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- 2016
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26. A Fully Microfabricated Electrochemical Sensor And Its Implementation For Detection Of Methicillin Resistance In Staphylococcus Aureus
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Koydemir, Hatice Ceylan, Kulah, Haluk, Alp, Alpaslan, Uner, Aysegul H., Hascelik, Gulsen, and Ozgen, Canan
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On-chip detection of biological analytes can enable diagnosis at the point of care. Combining the advantages of microelectromechanical system (MEMS) technology and molecular methods, we present the design of an integrated microfluidic platform, a microelectrochemical sensor (mu ECS), and its implementation for the detection of methicillin resistance in Staphylococcus aureus. This platform is capable of electrochemically sensing the target analyte in a microfluidic reactor without the usage of bulky electrodes, rendering it useful for in vitro diagnostics. In our experiments, the functionality of the sensor was tested for detecting specific DNA sequences of mecA gene (an indicator of methicillin resistance) over a range of concentrations of DNA (down to 10 pM). Synthetic oligonucleotides and bacterial PCR product were used as a target analyte in Hoechst 33258 marker-based detection and horseradish peroxidase-based detection, respectively. The results revealed that this platform has high sensitivity and selectivity. Also, its compatibility to MEMS processes enables its use with different applications ranging from detecting various types of cancers to endemics. The designed mu ECS can enable the detection of biological analytes of interest at low cost and high throughput.
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- 2014
27. Distribution of Spoligotyping Defined Genotypic Lineages among Drug-Resistant Mycobacterium tuberculosis Complex Clinical Isolates in Ankara, Turkey
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Kisa, Ozgul, primary, Tarhan, Gulnur, additional, Gunal, Selami, additional, Albay, Ali, additional, Durmaz, Riza, additional, Saribas, Zeynep, additional, Zozio, Thierry, additional, Alp, Alpaslan, additional, Ceyhan, Ismail, additional, Tombak, Ahmet, additional, and Rastogi, Nalin, additional
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- 2012
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28. Therapeutic effects of Mycobacterium phlei cell wall extracts
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ORHAN, BAĞNU, primary, BÜBER, ESRA, primary, KESKİN, MEHMET SELÇUK, primary, ÇELİKBIÇAK, ÖMÜR, primary, SALİH, BEKİR, primary, ALP, ALPASLAN, primary, SARIBAŞ, ZEYNEP, primary, ÖZEN, ABDURRAHİM HALUK, primary, and AÇAN, NACİYE LEYLA, primary
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- 2012
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29. In vitroand in vivofitness costs associated with Mycobacterium tuberculosisRpoB mutation H526D
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Rifat, Dalin, Campodónico, Victoria L, Tao, Jing, Miller, James A, Alp, Alpaslan, Yao, Yufeng, and Karakousis, Petros C
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Aim:There is controversy regarding the potential fitness costs of rifampicin (RIF) resistance-conferring mutations in the Mycobacterium tuberculosis(Mtb) rpoBgene. We characterized the pathogenicity of an Mtb RpoB H526D mutant. Materials & methods:A mutant containing the RpoB H526D mutation was isolated from wild-type Mtb grown on RIF-containing plates and complemented for determination of in vitroand in vivofitness costs. Results:The RpoB H526D mutant showed reduced survival relative to control strains during progressive hypoxia and delayed growth following resuscitation from nutrient starvation (p < 0.05), which was associated with reduced expression of the resuscitation-promoting factor genes rpfB, rpfCand rpfE. Relative to the isogenic wild-type strain, the mutant showed significantly attenuated growth and long-term survival as well as reduced inflammation in mouse lungs. Conclusion & future perspective:Our data suggest that RpoB H526D mutation confers a fitness cost during growth-limiting conditions in vitroand in mouse lungs.
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- 2017
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30. Rapid Determination of Rifampin Resistance in Clinical Isolates of Mycobacterium tuberculosis by Real-Time PCR
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Kocagoz, Tanil, primary, Saribas, Zeynep, additional, and Alp, Alpaslan, additional
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- 2005
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31. Genotyping of Mycobacterium tuberculosis clinical isolates in two cities of Turkey: Description of a new family of genotypes that is phylogeographically specific for Asia Minor
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Zozio, Thierry, primary, Allix, Caroline, additional, Gunal, Selami, additional, Saribas, Zeynep, additional, Alp, Alpaslan, additional, Durmaz, Riza, additional, Fauville-Dufaux, Maryse, additional, Rastogi, Nalin, additional, and Sola, Christophe, additional
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- 2005
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32. Rapid Detection of Rifampin Resistance in Mycobacterium tuberculosis Isolates by Heteroduplex Analysis and Determination of Rifamycin Cross-Resistance in Rifampin-Resistant Isolates
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Sarıbaş, Zeynep, primary, Kocagöz, Tanıl, additional, Alp, Alpaslan, additional, and Günalp, Ayfer, additional
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- 2003
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33. Rapid Determination of Rifampin Resistance in Clinical Isolates of Mycobacterium tuberculosisby Real-Time PCR
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Kocagoz, Tanil, Saribas, Zeynep, and Alp, Alpaslan
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ABSTRACTReal-time PCR was used to determine rifampin resistance in clinical isolates of Mycobacterium tuberculosis. Ninety-six rifampin-resistant isolates and 23 rifampin-susceptible isolates were included in the study. A 305-bp region covering the 81-bp “rifampin resistance-determining region” of rpoBwas amplified. Two hybridization probe pairs that covered the most frequent mutation sites in rpoB, codon regions 526 to 531 and 513 to 516, were used. The results obtained by real-time PCR were compared to those obtained by the proportion method. For detection of rifampin resistance, the real-time PCR assay yielded a sensitivity of 92.7% and a specificity of 100%. Real-time PCR is a very rapid method, and it can be especially helpful for the reporting of resistant clinical isolates in a very short period of time.
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- 2005
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34. Hacettepe Üniversitesi Hastanelerinde Görevli Sağlık Çalışanlarından Koronavirüs Hastalığı-2019 için Test Edilenlerin Özellikleri.
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Sertçelik, Ahmet, Dizman, Gülçin Telli, Atılmış, Duygu, Şahan, Ceyda, Kalaycı, Defne, Alp, Alpaslan, Çakır, Banu, Zarakol, Işkın Pınar, and Metan, Gökhan
- Abstract
Copyright of Mediterranean Journal of Infection, Microbes & Antimicrobials is the property of Galenos Yayinevi Tic. LTD. STI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2021
35. Hepatitis B virus screening and immunization rates in special risk groups.
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Balaban, Yasemin, Aslan, Abdullah Tarık, Akdoğan, Fatma Nur, Alp, Alpaslan, Ayar, Osman Dağ,Şefika, Mustafayev, Fuad, Şimsek, Cem, Vahabov, Javansir, Yıldırım, Tolga, Göker, Hakan, Ergunay, Koray, Hasçelik, Gülşen, Erdem, Yunus, Büyükaşık, Yahya, Purnak, Tugrul, and Şimşek, Halis
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- 2019
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36. Hepatitis E virus epidemiology of patients with chronic hepatitis C in Turkey.
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Balaban, Yasemin, Aslan, Abdullah Tarık, Akdoğan, Fatma Nur, Alp, Alpaslan, Ayar, Osman Dağ,Şefika, Mustafayev, Fuad, Şimşek, Cem, Vahabov, Javansir, Yıldırım, Tolga, Göker, Hakan, Ergünay, Koray, Hasçelik, Gülşen, Erdem, Yunus, Büyükaşık, Yahya, Purnak, Tuğrul, and Şimşek, Halis
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- 2019
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37. Rapid Detection of Rifampin Resistance in Mycobacterium tuberculosisIsolates by Heteroduplex Analysis and Determination of Rifamycin Cross-Resistance in Rifampin-Resistant Isolates
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Saribas¸, Zeynep, Kocago¨z, Tanil, Alp, Alpaslan, and Gu¨nalp, Ayfer
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ABSTRACTDirect heteroduplex analysis and a universal heteroduplex generator assay were performed to detect rifampin resistance rapidly in Turkish Mycobacterium tuberculosisisolates. Cross-resistance to rifapentine, rifabutin, and rifalazil was investigated. A relationship between specific mutations and resistance patterns, which can guide the choice of an appropriate therapeutic regimen for tuberculosis patients, was identified.
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- 2003
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38. The Determination of S-aureus and S.aureus Enterotoxin -A Producing Strain by Real-Time PCR at Meatball Meals of Two Hospitals in Ankara, Turkey
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Uyar, Muhemmet Fatih, Alp, Alpaslan, Hasan Cenk Mirza, Ruh, Emrah, Kizil, Mevlude, Dikmen, Derya, Buyuktuncer, Zehra, Sener, Burcin, and Beyhan, Yasemin
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This research was planned and carried out in order to determine Staphylococcus aureus and Staphylococcal Enterotoxin A presence quickly in meatball meals cooked in two different hospitals with two different cooking systems by using molecular and classical microbiological methods. Total 60 samples of meals with meatballs (30 samples from each hospital) were included in the research. S.aureus and S.aureus Enterotoxin A producing bacteria was detected in 6.6% of the samples of hospital A which used new production techniques by using both molecular and classic microbiologic methods. S.aureus and S.aureus Enterotoxin A producing bacteria was detected in 9.9% of samples of Hospital B which used traditional methods by using both microbiologic methods. No statistical difference was found out between two microbiologic methods in terms of S.aureus identification. Consequently, hygienic rules must be obeyed during all phases, the conditions must be improved and sustainable training of personnel must be ensured in order to prevent food poisoning cases in hospital food service systems. Also, molecular microbiologic methods used for determination of nutrient pathogens are faster and more functional, so usage of these methods is crucial.
39. Mycobacterium tuberculosis klinik izolatlarında yeni antitüberküloz ilaçlar olan bedaquiline ve Delamanid'in MİK değerlerinin ve dirence neden olan mutasyonların saptanması
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Akdoğan Kittana, Fatma Nur, Alp, Alpaslan, and Tıbbi Mikrobiyoloji Anabilim Dalı
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Mikrobiyoloji ,Drug resistance ,Mycobacterium tuberculosis ,Microbiology - Abstract
Bu çalışmanın amacı, dirençli tüberküloz hastalarından izole edilen 101 M. tuberculosis klinik izolatının bedaquiline ve delamanid minimal inhibitör konsantrasyon (MİK) değerlerinin fenotipik olarak MGIT-960 otomatize sıvı kültür sisteminde bir araştırma yazılımı olan TB/eXİST ile saptanması ve dirence neden olan mutasyonların Sanger dizileme ve tüm genom analizi (WGS) yöntemleriyle genotipik olarak araştırılmasıdır. Suşların 31'i İsviçre Zürih Üniversitesi'nde, 70'i ise Türkiye'deki hastalardan izole edilmiştir. İsviçre suşlarında bedaquiline ve klofazimin arasında çapraz direnç varlığı da araştırılmıştır. Fenotipik duyarlılık sonuçlarına göre İsviçre ve Türkiye suşlarının bedaquiline, delamanid ve yalnızca İsviçre suşlarının klofazimin MİK dağılımları ve ortalama MİK değerleri sırasıyla
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- 2020
40. The microbiological diagnosis of tuberculous meningitis: results of Haydarpasa-1 study
- Author
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Ebru Kurşun, Derya Ozturk-Engin, Branislava Savic, Rok Čivljak, Bojana Beović, Mehmet Parlak, Elif Sahin-Horasan, Oguz Karabay, Selma Alabay, Emine Parlak, Esmeray Mutlu Yilmaz, Ahmad Khalifa, Uner Kayabas, Saim Dayan, Hakan Erdem, Sukran Kose, V Kirova, Jean-Paul Stahl, Gonul Sengoz, Bruno Cacopardo, Canan Agalar, Arjan Harxhi, Gorana Cosic, Yves Hansmann, Catalina Luca, Alpaslan Alp, Aysegul Ulu-Kilic, Mehmet Bitirgen, Kadriye Kart Yaşar, Özcan Deveci, Isik Somuncu Johansen, Mustafa Namiduru, Katell Andre, I Masse-Chabredier, H. Cem Gul, Serkan Oncu, Mustafa Sunbul, Akram Al-Mahdawi, Ayhan Akbulut, Gulden Yilmaz, Serda Gulsun, Oral Oncul, S Chadapaud, Soline Simeon, Melanie Catroux, Oğuz Reşat Sipahi, Recep Tekin, Ayşe Seza Inal, Mucahit Yemisen, Filiz Pehlivanoglu, Olga Dulovic, Asuman Inan, B. Lakatos, Bahar Kandemir, Nazif Elaldi, Ghaydaa A. Shehata, Ahmet Karakaş, Alper Şener, Mihai Nechifor, Muge Ozguler, Alexandru Crisan, Nurgul Ceran, Çukurova Üniversitesi, OMÜ, Ege Üniversitesi, [Erdem, H. -- Oncul, O.] GATA Haydarpasa Training Hosp, Dept Infect Dis & Clin Microbiol, Istanbul, Turkey -- [Ozturk-Engin, D. -- Inan, A. -- Ceran, N.] Haydarpasa Numune Training & Res Hosp, Dept Infect Dis & Clin Microbiol, Istanbul, Turkey -- [Elaldi, N.] Cumhuriyet Univ Sch Med, Dept Infect Dis & Clin Microbiol, Sivas, Turkey -- [Gulsun, S.] Diyarbakir Training & Res Hosp, Dept Infect Dis & Clin Microbiol, Diyarbakir, Turkey -- [Sengoz, G. -- Pehlivanoglu, F.] Haseki Training & Res Hosp, Dept Infect Dis & Clin Microbiol, Istanbul, Turkey -- [Crisan, A.] Victor Babes Univ Med & Pharm, Dept Infect Dis, Timisoara, Romania -- [Johansen, I. S.] Odense Univ Hosp, Dept Infect Dis Q, DK-5000 Odense, Denmark -- [Nechifor, M.] Gr T Popa Univ Med & Pharm, Dept Pharmacol, Iasi, Romania -- [Al-Mahdawi, A.] Baghdad Teaching Hosp, Dept Neurol, Baghdad, Iraq -- [Civljak, R.] Univ Zagreb Sch Med, Dr Fran Mihaljev Univ Hosp Infect Dis, Dept Infect Dis, Zagreb, Croatia -- [Ozguler, M. -- Akbulut, A.] Firat Univ Sch Med, Dept Infect Dis & Clin Microbiol, Elazig, Turkey -- [Savic, B.] Univ Belgrade, Inst Microbiol & Immunol, Natl Reference Lab TB, Fac Med, Belgrade, Serbia -- [Cacopardo, B.] Univ Catania, Infect Dis Sect, Dept Clin & Mol Biomed, Catania, Italy -- [Inal, A. S.] Cukurova Univ Sch Med, Dept Infect Dis & Clin Microbiol, Adana, Turkey -- [Namiduru, M.] Gaziantep Univ Sch Med, Dept Infect Dis & Clin Microbiol, Gaziantep, Turkey -- [Dayan, S. -- Tekin, R. -- Deveci, O.] Dicle Univ Sch Med, Dept Infect Dis & Clin Microbiol, Diyarbakir, Turkey -- [Kayabas, U.] Inonu Univ Sch Med, Dept Infect Dis & Clin Microbiol, Malatya, Turkey -- [Parlak, E. -- Parlak, M.] Ataturk Univ Sch Med, Dept Infect Dis & Clin Microbiol, Erzurum, Turkey -- [Khalifa, A.] Damascus Hosp, Dept Neurol, Damascus, Syria -- [Kursun, E.] Baskent Univ Sch Med, Dept Infect Dis & Clin Microbiol, Adana, Turkey -- [Sipahi, O. R.] Ege Univ Sch Med, Dept Infect Dis & Clin Microbiol, Izmir, Turkey -- [Yemisen, M.] Istanbul Univ Cerrahpasa Sch Med, Dept Infect Dis & Clin Microbiol, Istanbul, Turkey -- [Bitirgen, M. -- Kandemir, B.] Necmettin Erbakan Univ Sch Med, Dept Infect Dis & Clin Microbiol, Konya, Turkey -- [Dulovic, O.] Univ Belgrade, Clin Infect & Trop Dis, Clin Ctr Serbia, Fac Med, Belgrade, Serbia -- [Luca, C.] Gr T Popa Univ Med & Pharm, Dept Infect Dis, Iasi, Romania -- [Stahl, J. P.] Joseph Fourier Univ & Univ Hosp Grenoble, Dept Infect Dis, Grenoble, France -- [Simeon, S.] Univ Hosp Pontchaillou, Dept Infect & Trop Dis, Rennes, France -- [Ulu-Kilic, A. -- Alabay, S.] Erciyes Univ Sch Med, Dept Infect Dis & Clin Microbiol, Kayseri, Turkey -- [Yasar, K.] Bakrkoy Dr Sadi Konuk Training & Res Hosp, Dept Infect Dis & Clin Microbiol, Istanbul, Turkey -- [Yilmaz, G.] Ankara Univ Sch Med, Dept Infect Dis & Clin Microbiol, Ankara, Turkey -- [Yilmaz, E.] Uludag Univ Sch Med, Dept Infect Dis & Clin Microbiol, Bursa, Turkey -- [Beovic, B.] Univ Med Ctr, Dept Infect Dis, Ljubljana, Slovenia -- [Catroux, M.] Univ Poitiers Hosp, Dept Infect Dis, Poitiers, France -- [Lakatos, B.] St Laszlo Hosp, Dept Infect Dis, Budapest, Hungary -- [Sunbul, M.] Ondokuz Mayis Univ Sch Med, Dept Infect Dis & Clin Microbiol, Samsun, Turkey -- [Sahin-Horasan, E.] Mersin Univ Sch Med, Dept Infect Dis & Clin Microbiol, Mersin, Turkey -- [Kose, S.] Tepecik Training & Res Hosp, Dept Infect Dis & Clin Microbiol, Izmir, Turkey -- [Shehata, G.] Assiut Univ Hosp, Dept Neurol & Psychiat, Assiut, Egypt -- [Andre, K.] Dax Hosp, Dept Infect Dis, Dax, France -- [Alp, A.] Hacettepe Univ, Sch Med, Deparment Med Microbiol, Ankara, Turkey -- [Cosic, G.] Univ Novi Sad, Fac Med, Dept Prevent & Control Dis, IPH Vojvodina, Novi Sad, Serbia -- [Gul, H. Cem -- Karakas, A.] Gulhane Mil Med Acad, Dept Infect Dis & Clin Microbiol, Ankara, Turkey -- [Chadapaud, S.] Marie Jose Treffot Hosp, Dept Infect Dis, Hyeres, France -- [Hansmann, Y.] Univ Hosp, Dept Infect Dis, Strasbourg, France -- [Harxhi, A.] Univ Hosp Ctr Tirana, Infect Dis Serv, Tirana, Albania -- [Kirova, V.] Univ Clin Infect Dis & Febrile Condit, Skopje, Macedonia -- [Masse-Chabredier, I.] Aurillac Hosp, Dept Infect Dis, Aurillac, France -- [Oncu, S.] Adnan Menderes Univ Sch Med, Dept Infect Dis & Clin Microbiol, Aydin, Turkey -- [Sener, A.] Onsekiz Mart Univ Sch Med, Dept Infect Dis & Clin Microbiol, Canakkale, Turkey -- [Karabay, O.] Sakarya Univ Sch Med, Dept Infect Dis & Clin Microbiol, Sakarya, Turkey -- [Agalar, C.] Fatih Sultan Mehmet Training & Res Hosp, Dept Infect Dis & Clin Microbiol, Istanbul, Turkey, Inal, Ayse Seza -- 0000-0002-1182-7164, Ghaydaa, Shehata -- 0000-0002-3631-893X, johansen, isik somuncu -- 0000-0002-2189-9823, Karabay, Oguz -- 0000-0003-0502-432X, Karakas, Ahmet -- 0000-0002-0553-8454, Kart Yasar, Kadriye -- 0000-0003-2963-4894, Stahl, Jean Paul -- 0000-0002-0086-3557, Elaldi, Nazif -- 0000-0002-9515-770X, ALP, ALPASLAN -- 0000-0001-7856-7590, and Civljak, Rok -- 0000-0001-8766-7438
- Subjects
Microbiology (medical) ,Adult ,Male ,medicine.medical_specialty ,Adolescent ,QUANTIFERON-TB GOLD ,diagnosis ,Adenosine Deaminase ,Culture ,Tuberculous meningitis ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Internal medicine ,Diagnosis ,medicine ,Tuberculosis ,Humans ,Meningitis ,030212 general & internal medicine ,Precision Medicine ,Aged ,Retrospective Studies ,culture ,meningitis ,PCR ,tuberculosis ,Aged, 80 and over ,Bacteriological Techniques ,business.industry ,General Medicine ,Mycobacterium tuberculosis ,Middle Aged ,medicine.disease ,3. Good health ,Surgery ,Löwenstein–Jensen medium ,Infectious Diseases ,Early Diagnosis ,Tuberculosis, Meningeal ,Positive culture ,Female ,business ,030217 neurology & neurosurgery ,Interferon-gamma Release Tests - Abstract
WOS: 000345825900004, PubMed ID: 24849547, We aimed to provide data on the diagnosis of tuberculous meningitis (TBM) in this largest case series ever reported. The Haydarpasa-1 study involved patients with microbiologically confirmed TBM in Albania, Croatia, Denmark, Egypt, France, Hungary, Iraq, Italy, Macedonia, Romania, Serbia, Slovenia, Syria and Turkey between 2000 and 2012. A positive culture, PCR or Ehrlich-Ziehl-Neelsen staining (EZNs) from the cerebrospinal fluid (CSF) was mandatory for inclusion of meningitis patients. A total of 506 TBM patients were included. The sensitivities of the tests were as follows: interferon- release assay (Quantiferon TB gold in tube) 90.2%, automated culture systems (ACS) 81.8%, Lowenstein Jensen medium (L-J) 72.7%, adenosine deaminase (ADA) 29.9% and EZNs 27.3%. CSF-ACS was superior to CSF L-J culture and CSF-PCR (p
- Published
- 2013
41. Yataklı tedavi kurumlarında farklı iki yöntemle üretilen köfteli yemeklerdeki Staphylococcus aureus ve Stafilokokal Enterotoksin A varlığının moleküler mikrobiyolojik yöntemlerle saptanması
- Author
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Uyar, Muhemmet Fatih, Beyhan, Yasemin, Alp, Alpaslan, and Beslenme ve Diyetetik Anabilim Dalı
- Subjects
Staphylococcus aureus ,Nutrition and Dietetics ,Mikrobiyoloji ,Beslenme ve Diyetetik ,Microbiology - Abstract
Bu araştırma iki farklı yemek üretimi ile hizmet veren iki farklı yataklı tedavi kurumunda (A kurumu ? kendi personeli ile yeni üretim sistemi, B kurumu ? özel firmadan yerinde geleneksel üretim sistemi) üretilen köfteli yemeklerde Staphylococcus aureus ve Enteroktoksin A varlığının moleküler mikrobiyolojik yöntemlerle hızlı bir şekilde tanımlanması amacıyla planlanmış ve yürütülmüştür. Çalışmaya her kurumdan 30'ar olmak üzere toplam 60 köfteli yemek örneği dahil edilmiştir. Araştırma sonunda, geleneksel üretim sistemiyle hizmet veren B kurumunun mutfak bölümlerden et hazırlama ve sebze hazırlama bölümlerinin yüzey toplam bakteriyel yük skorları sırasıyla A=610.80 ? B=26745, A=62.40 ? B=5316 olmak üzere A kurumuna göre anlamlı şekilde (p0.05). Sonuç olarak, toplu beslenme sistemlerinde besin zehirlenmelerinin önüne geçilebilmesi için tüm aşamalarda hijyen kurallarına uyulması gerekmektedir. Bunun için de koşulların iyileştirilmesinin yanı sıra personelin eğitimlerinin sürekliliği sağlanmalıdır. Aynı zamanda besin patojenlerinin saptanması için daha hızlı ve pratik olması açısından moleküler mikrobiyolojik yöntemlerin kullanımının önemli olduğu sonucuna da varılmıştır. This research was planned and carried out in order to determine Staphylococcus aureus and Staphylococcal Enterotoxin A presence quickly in meatball meals cooked in two different hospitals (hospital A- new production system with its own staff, hospital B-traditional production system from private sector) with two different cooking systems by using molecular and classical microbiological methods. Total 60 samples of meals with meatballs (30 samples from each hospital) were included in the research. At the end of the research, it was found out that surface total bacterial load scores (respectively; A=610.80 ? B=26745, A=62.40 ? B=5316) of meat and vegetable preparation areas in the kitchen of hospital B which used traditional cooking systems were considerably (p
- Published
- 2009
42. Mycobacterium tuberculosis klinik izolatlarında kinolon direncinin klasik ve moleküler yöntemlerle saptanması
- Author
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Ruh, Emrah, Alp, Alpaslan, and Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı
- Subjects
Mikrobiyoloji ,Ciprofloxacin ,Clinical Microbiology and Infectious Diseases ,Klinik Bakteriyoloji ve Enfeksiyon Hastalıkları ,Tuberculosis ,Mycobacterium tuberculosis ,Microbiology - Abstract
Tüberküloz hastalığı, tedavide kullanılan birincil ilaçlara karşı gelişen dirençten dolayı günümüzde önemli bir sağlık sorunu olmaya devam etmektedir. Dünya Sağlık Örgütü, özellikle çok ilaca dirençli olgularda umut vaad eden kinolon grubu antibiyotiklerin kullanılmasını önermektedir. Bu çalışmaya, 59'u çok ilaca dirençli, 41'i birincil ilaçlardan en az birine dirençli olan 100 Mycobacterium tuberculosis klinik izolatı dahil edilerek, siprofloksasin, sparfloksasin ve moksifloksasinin bu izolatlara olan etkinliği test edilmiştir. Agar dilüsyon yöntemi sonucunda üç kinolon birlikte değerlendirildiğinde, 100 izolatın 6'sının dirençli, 35'inin orta derecede dirençli, 59'unun ise duyarlı olduğu belirlenmiştir. MİK değerleri ile dirençli izolatlar ve orta derecede dirençli izolatların sayıları dikkate alındığında, sparfloksasin ve moksifloksasinin, siprofloksasine göre daha etkili olduğu görülmüştür. Mycobacterium tuberculosis'de kinolon direncine en sık neden olan gyrA genindeki mutasyonların saptanması için DNA dizi analizi yapılmış ve 6 dirençli izolatın birinde Ala90Glu ve Asp94Gly, diğerinde ise Met81Leu mutasyonu saptanmıştır. Orta derecede dirençli olan izolatların ise birinde Tyr84Asp, diğerinde His87Asn mutasyonu saptanmıştır. Ser95Thr polimorfizmi, DNA dizi analizi yapılan izolatların %80'inde saptanmıştır. Ayrıca, bu izolatların % 73'ünde saptanan Asp120Gly dönüşümünün 4 duyarlı izolatın 3'ünde de görülmesi, bunun da bir polimorfizm olduğunu düşündürmektedir. Dirençli ve orta derecede dirençli izolatların çoğunda, sadece bu polimorfizmlerin oluşması, özellikle düşük düzey dirençli izolatlarda kinolon direncinin, ilacın hücre içinde birikimini engelleyen başka bir mutasyon sonucu oluştuğu fikrini vermektedir. Bu çalışmada ayrıca, kısa sürede sonuç verebilen floresans rezonans enerji transferi teknolojisinin uygulandığı real-time PCR yönteminin Mycobacterium tuberculosis'de kinolon direncini belirlemedeki etkinliği araştırılmıştır. Dirençli, orta derecede dirençli ve duyarlı izolatların EST değerlerinin benzer oranlarda dağılım göstermesinden dolayı bu yöntemin kinolon direncini saptama konusunda tek başına yeterli olmadığı sonucuna varılmıştır. Tuberculosis continues to be a serious health issue due to the resistance of the infecting organism against the first line drugs being used in the therapy. World Health Organisation recommends the fluoroquinolones as alternative optimising drugs especially in the treatment of multi-drug resistant cases. One hundred Mycobacterium tuberculosis clinical isolates, of which 59 were multi-drug resistant and 41 were resistant to at least one of the first line drugs, were included in this study and the activity of ciprofloxacin, sparfloxacin and moxifloxacin against these isolates was studied. By the agar proportion method, 6 isolates were determined as resistant, and 35 were classified as moderately resistant. The remaining 59 isolates were susceptible against the fluoroquinolones tested. According to the MIC results and the number of resistant and moderately resistant isolates, sparfloxacin and moxifloxacin were found to be more active than ciprofloxacin. In order to detect the mutations in the gyrA gene, which remains as the major cause of the fluoroquinolone resistane, DNA sequencing procedure was performed. Among the 6 resistant isolates, Ala90Glu and Asp94Gly were detected in one isolate and Met81Leu in the other. Tyr84Asp was detected in one isolate and His87Asn was detected in the other among the moderately resistant isolates. The natural Ser95Thr polymorphism was detected in 80% of the isolates sequenced. Moreover, Asp120Gly which was detected in 73% of these isolates, was also detected in 3 of 4 susceptible isolates, suggesting that this is another polymorphism. The detection of these polymorphisms as the most common mutations also suggests that, fluoroquinolone resistance may possibly develop due to another mechanism which prevents the accumulation of the drug in the cell. In this study, the ability of real-time PCR- fluorescence resonance energy transfer technology in detection of fluoroquinolone resistance of Mycobacterium tuberculosis was also examined. Since the melting temperatures of resistant, moderately resistant and susceptible isolates were similar to each other, this method alone was not able to detect the fluoroquinolone resistance. 97
- Published
- 2008
43. Identification of Malassezia Species in Basal Cell Carcinoma Lesions by Conventional and Molecular Methods.
- Author
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Koç Yıldırım S, Akdoğan Kıttana FN, Ersoy Evans S, Alp A, Arikan-Akdagli S, and Karaduman A
- Published
- 2024
- Full Text
- View/download PDF
44. Epstein-Barr Virus-Positive Leiomyosarcoma in Immunocompetent Patients.
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Al-Tarawneh H, Alp A, Gedikoglu G, and Kosemehmetoglu K
- Subjects
- Female, Humans, Male, Herpesvirus 4, Human genetics, Immunocompromised Host, Epstein-Barr Virus Infections complications, Epstein-Barr Virus Infections pathology, Leiomyosarcoma pathology, Smooth Muscle Tumor pathology
- Abstract
Objective: Epstein-Barr Virus-Associated Smooth Muscle Tumor (EBV-SMT) is a rare tumor with a higher rate of occurrence in unusual locations in the setting of immunodeficiency. In this study, we evaluated a cohort of ordinary leiomyosarcomas (LMS) for the presence of EBV and described the clinicopathological features deviating from routinely diagnosed cases of EBV-SMT., Material and Method: The sections of tissue microarrays including 93 classical LMS occurring in various locations were hybridized with EBER and stained for LMP1 antibody using the Leica Bond Autostainer. EBV real-time PCR assay was performed in 2 EBER-positive cases., Results: Among the 93 LMS cases, 2 non-uterine cases (2.2%) were positive for EBER and negative for LMP1, and were referred to as `EBV-positive LMS`. Both were females in their 6th decade without immunosuppression. EBV real-time PCR assay revealed the presence of EBV in one of the cases. Tumors were located in the pancreas and chest wall. Morphologically, tumors were rather myxoid, multinodular, and composed of long fascicles of spindle cells with intermediate- to high-grade features. High mitotic activity and focal necrosis were present, whereas no accompanying lymphocytes were detected. One of the patients developed metastatic disease after 3 years., Conclusion: EBV-positive LMS occurring in immunocompetent patients has features distinct from classical EBV-SMT seen in immunosuppressed patients.
- Published
- 2024
- Full Text
- View/download PDF
45. Outcome of noncritical COVID-19 patients with early hospitalization and early antiviral treatment outside the ICU
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Çalık Başaran N, Uyaroğlu OA, Telli Dizman G, Özışık L, Şahin TK, Taş Z, İnkaya AÇ, Karahan S, Alp Ş, Alp A, Metan G, Zarakol P, Sain Güven G, Öz ŞG, Topeli A, Uzun Ö, Akova M, and Ünal S
- Subjects
- Adult, Aged, Aged, 80 and over, Drug Therapy, Combination, Early Medical Intervention, Early Warning Score, Female, Humans, Intensive Care Units, Length of Stay, Male, Middle Aged, Prospective Studies, SARS-CoV-2, Severity of Illness Index, Treatment Outcome, Young Adult, Amides therapeutic use, Anti-Bacterial Agents therapeutic use, Antiviral Agents therapeutic use, Azithromycin therapeutic use, Hospitalization, Hydroxychloroquine therapeutic use, Pyrazines therapeutic use, COVID-19 Drug Treatment
- Abstract
Background/aim: Despite the fact that the COVID-19 pandemic has been going on for over 5 months, there is yet to be a standard management policy for all patients including those with mild-to-moderate cases. We evaluated the role of early hospitalization in combination with early antiviral therapy with COVID-19 patients in a tertiary care university hospital., Materials and Methods: This was a prospective, observational, single-center study on probable/confirmed COVID-19 patients hospitalized in a tertiary care hospital on COVID-19 wards between March 20 and April 30, 2020. The demographic, laboratory, and clinical data were collected., Results: We included 174 consecutive probable/confirmed COVID-19 adult patients hospitalized in the Internal Medicine wards of the University Adult Hospital between March 20 and April 30, 2020. The median age was 45.5 (19–92) years and 91 patients (52.3%) were male. One hundred and twenty (69%) were confirmed microbiologically, 41 (23.5%) were radiologically diagnosed, and 13 (7.5%) were clinically suspected (negative microbiological and radiological findings compatible with COVID-19); 35 (20.1%) had mild, 107 (61.5%) moderate disease, and 32 (18.4%) had severe pneumonia. Out of 171 cases, 130 (74.3%) showed pneumonia; 80 were typical, and 50 showed indeterminate infiltration for COVID-19. Patients were admitted within a median of 3 days (0-14 days) after symptoms appear. The median duration of hospitalization was 4 days (0-28 days). In this case series, 13.2% patients were treated with hydroxychloroquine alone, 64.9% with hydroxychloroquine plus azithromycin, and 18.4% with regimens including favipiravir. A total of 15 patients (8.5%) were transferred to the ICU. Four patients died (2.2%)., Conclusion: In our series, 174 patients were admitted to the hospital wards for COVID-19, 69% were confirmed with PCR and/or antibody test. At the time of admission, nearly one fifth of the patients had severe diseases. Of the patients, 95.4% received hydroxychloroquine alone or in combination. The overall case fatality rate was 2.2%., Competing Interests: The authors declare that they have no conflict of interest. This study was carried out without any support from funding agencies in the public, commercial, or nonprofit sectors., (This work is licensed under a Creative Commons Attribution 4.0 International License.)
- Published
- 2021
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- View/download PDF
46. Is COVID-19 a risk factor for invasive pulmonary aspergillosis in critically ill patients?
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Er B, Er AG, Metan G, Halaçlı B, Ortaç Ersoy E, Hazırolan G, Alp A, Sarıbaş Z, Arıkan Akdağlı S, Topeli A, and Uzun Ö
- Published
- 2021
- Full Text
- View/download PDF
47. Out-patient management of patients with COVID-19 on home isolation.
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Ayaz CM, Dizman GT, Metan G, Alp A, and Unal S
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- Adult, Bed Occupancy, COVID-19, COVID-19 Testing, Clinical Laboratory Techniques methods, Coronavirus Infections diagnosis, Coronavirus Infections epidemiology, Coronavirus Infections transmission, Female, Health Personnel statistics & numerical data, Humans, Male, Occupational Diseases diagnosis, Occupational Diseases drug therapy, Occupational Diseases epidemiology, Outpatients, Pandemics, Pneumonia, Viral diagnosis, Pneumonia, Viral epidemiology, Pneumonia, Viral transmission, Retrospective Studies, SARS-CoV-2, Symptom Assessment, Turkey epidemiology, Ambulatory Care methods, Antiviral Agents therapeutic use, Betacoronavirus, Coronavirus Infections drug therapy, Patient Isolation methods, Pneumonia, Viral drug therapy
- Abstract
The rapidly increased number of patients with COVID-19 resulted in the shortage of hospital beds. An outpatient follow-up plan was developed for COVID-19 patients with stable clinical condition and no concomitant diseases. The records of COVID-19 first admission clinic were retrospectively reviewed to identify the COVID-19 patients who were followed on home isolation as outpatients between March 17, 2020 and April 18, 2020 in Ankara, Turkey. Demographic and clinical characteristics of the patients, compliance with isolation rules, re-admission rates, and outcomes were investigated. A total of 41 patients with COVID-19 were followed on home isolation without hospitalization. The median age of the patients was 36 years. Twenty-four (58.5%) of 41 patients were female. Twenty-nine (70.7%) patients were healthcare workers. The most common symptoms at admission were cough, myalgia/arthralgia, and loss of smell and/or taste. Fourteen (34.1%) patients were asymptomatic on the first admission. Anti-viral treatment was given to 27 (65.8%) of 41 patients. Four of 41 patients were readmitted to the outpatient clinic and hospitalized. Three patients had worsening respiratory symptoms and pneumonia was detected in CT scans. One patient was hospitalized because of disseminated herpes zoster infection. Two patients who jeopardized the isolation rules were isolated and monitored at another hospital by provincial health directorate teams. If adequate conditions are provided, follow-up on home isolation seems to be a feasible method in carefully selected patients. However, these patients should be monitored closely by an experienced team during the isolation period.
- Published
- 2020
48. Advancement in POCT Molecular Testing: The Multiplex PCR POCT Devices for Infectious Diseases.
- Author
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Alp A
- Abstract
Rapid and accurate diagnostic tests are very important for the global control of infectious diseases. The point of care diagnosis has become a promising strategy in recent years. Different kind of point of care testing devices has been introduced into the market in the last decade. These devices must provide a low-cost, robust, sensitive, specific, and practical analysis in order to replace the conventional clinical laboratory diagnostic test algorithms when needed. The successful implementation of point of care diagnostics has a potential to increase the strength of infectious diseases surveillance programs. Finally, the rapid progress in point of care diagnosis can stimulate a shift from a centralized diagnostic model to a decentralized patient-centered approach.
- Published
- 2018
49. Rapid detection of rifampin resistance in Mycobacterium tuberculosis isolates by heteroduplex analysis and determination of rifamycin cross-resistance in rifampin-resistant isolates.
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Saribaş Z, Kocagöz T, Alp A, and Günalp A
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- Drug Resistance, Bacterial, Drug Resistance, Multiple, Humans, Microbial Sensitivity Tests, Mycobacterium tuberculosis isolation & purification, Antibiotics, Antitubercular pharmacology, Heteroduplex Analysis methods, Mycobacterium tuberculosis drug effects, Rifampin pharmacology, Rifamycins pharmacology
- Abstract
Direct heteroduplex analysis and a universal heteroduplex generator assay were performed to detect rifampin resistance rapidly in Turkish Mycobacterium tuberculosis isolates. Cross-resistance to rifapentine, rifabutin, and rifalazil was investigated. A relationship between specific mutations and resistance patterns, which can guide the choice of an appropriate therapeutic regimen for tuberculosis patients, was identified.
- Published
- 2003
- Full Text
- View/download PDF
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