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Xiao-Li Yang,1,&ast; Wen-Yao Xie,2,&ast; Yi-Min Cai,3 Hong-Ying Tang,1 Mei-Yi Tao,3 Zhou-Min Shen,3 Hong-Jiao Chen4 1Department of Liver Surgery, Hunan Provincial People’s Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha, 410005, People’s Republic of China; 2Interventional Operating Room, Hunan Provincial People’s Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha, 410005, People’s Republic of China; 3Department of Nursing, Hunan Provincial People’s Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha, 410005, People’s Republic of China; 4Medical College of Hunan Normal University, Changsha, 410013, People’s Republic of China&ast;These authors contributed equally to this studyCorrespondence: Yi-Min Cai, Nursing department, Hunan Provincial People’s Hospital (The First Affiliated Hospital of Hunan Normal University), No. 61, Jiefang West Road, Changsha, 410005, People’s Republic of China, Tel +86 13787129085, Fax +0731-83928047, Email cyimin2022@163.comPurpose: To understand existing negative emotions in patients with coronary heart disease after percutaneous coronary stent implantation (PCI) and analyse its influencing factors.Methods: Patients with coronary heart disease after PCI in three tertiary hospitals in Changsha City from April to September 2018 were selected as the research subjects. The self-designed general information questionnaire assessed irritability, depression and anxiety (IDA) on a self-rating scale. It was used to examine patients’ existing negative emotions with coronary heart disease after PCI and analyse the influencing factors.Results: 203 questionnaires were distributed, and 202 valid questionnaires were recovered, with an effective recovery rate of 99.5%. The IDA score of patients with coronary heart disease after PCI was 17.01± 7.60 points, the incidence of negative emotions was 63.8%, and the incidences of depression, anxiety and irritability were 39.6%, 8.4% and 15.8%, respectively. Negative emotion was taken as the dependent variable and a patient’s general data, such as lifestyle and disease, as the independent variables. A univariate analysis was conducted to obtain gender, age, educational level, marital status, work status, per capita monthly household income, sleep status, etc. Seven factors were identified as the influencing factors of negative emotions in patients with coronary heart disease after PCI, and the difference was statistically significant (P< 0.05).Conclusion: Most patients with coronary heart disease after PCI tend to exhibit negative emotions such as anxiety and depression. Medical staff should attach great importance to evaluating any negative feelings in this group and take timely targeted intervention measures to prevent and mitigate the occurrence and development of these adverse emotions in patients with coronary heart disease after PCI.Keywords: coronary heart disease, postoperative PCI, negative emotions, influencing factors

The aim of the present study was to investigate the PAQR3 gene expression and its methylation level in colorectal cancer tissues, as well as the association with colorectal cancer clinical data. In total, 54 cases of colorectal cancer tissue samples and normal adjacent tissue samples were collected between June, 2013 and July, 2014. RT-PCR and western blot analysis were used to detect the mRNA and protein levels of PAQR3 in colorectal samples, respectively. MSP was used to detect the methylation level of PAQR3 gene in colorectal samples, which was compared with colorectal data. The results showed that a decreased expression level of PAQR3 mRNA in colorectal cancer tissues and the expression reduction rate was 57.4% (31/54). Similarly, the expression level of PAQR3 protein was reduced in cancer tissues, and the reduction rate was 46.3% (25/54), while the protein expression reduction rate in cancer adjacent tissue was 5.6% (3/54), and the difference was statistically significant (P

A versatile strategy to fabricate newly-developed silica-modified membranes based on organic/inorganic hybridization for oil/water separation is described in this study. The modified membranes are easily obtained by a simple two-step immersion of polysulfone hollow fiber membranes with radial gradient microstructure (RGM-PSF) into a silicon solution of tetraethyl orthosilicate (TEOS) mixed with 3-glycidyloxypropyltrimethoxysilane (GOPTS) and subsequently into a lactic acid solution. Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS) and field emission scanning electron microscopy (FE-SEM) were utilized to characterize the surface compositions and microstructures. A micro/nano-scaled SiO2-based coating was generated roughly and continuously on the membrane surfaces, endowing them with superhydrophilicity and underwater superoleophobicity. Results of dead-end filtration experiments demonstrated that the hybridized RGM-PSF membranes could be applied in oil-in-water emulsion separation far more efficiently, with higher water permeate flux, better oil rejection ratio, and higher pressure endurance than those of the common ones. Therefore, this study has provided a facile approach to prepare silica-modified membranes with special wettability, showing prominent application prospects for oily wastewater treatment in practical processes.

Objective To evaluate the effect of low-concentration preoxygenation on physical work capacity with a heart rate of 170 beat per minute (PWC170) in recruits arrived on Qinghai-Tibet Plateau (3651m height above sea level), and investigate the possible correlation between new recruits' coping style and physical work capacity. Methods Thirty-nine recruits coming from plains (799m or 620m height above sea level) were involved in our study. They were randomly divided into 3 groups: experimental group I (n=12), experimental groupⅡ (n=12), and control group (n=15). The group I had 1L/min low concentration preoxygenation for 30min, and groupⅡ had 2L/min for 30min, once a day for 3 days. No preoxygenation in control group. The new recruits's PWC170 values were measured by stepping stairs for evaluating their physical ability, and the new recruits's coping responses were investigated with "Physical Training Coping Scale", and then those data compared with these of 98 soldiers stationed in Tibet (senior soldier group, stationed in Tibet for at least 2 years). Results Each basic PWC170 values showed no significant diffierence between group Ⅰand Ⅱ (P>0.05). Each basic PWC170 was significantly higher in the experimental groups Ⅰand Ⅱthan in control group (P0.05). Correlation analysis showed that there was a significant positive correlation between the "active response" factor and basic PWC170 value, and between "emotional management" factor and PWC170 value on second day between the two experimental groups (r=0.329 and 0.344 respectively, P

Objectives As a parenchymal cell, the photoreceptor is more susceptible to alterations in outer micro-environmental conditions than other cells. In the present study, we aimed to investigate inhibitory effects of zinc oxide (ZnO) nanoparticles on expression of manganese superoxide dismutase (MnSOD) in murine photoreceptor-derived cells. Materials and methods We investigated effects of ZnO nanoparticles on murine photoreceptor cell viability and on expression and activity of MnSOD using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, immunofluorescence analysis, flow cytometry, quantitative real-time PCR and enzyme-linked immunosorbent assay (ELISA). Results ZnO nanoparticles were found to have higher cytotoxic effects in concentration- and time-dependent manners, to elevate intracellular levels of hydrogen peroxide and hydroxyl radicals, and thus to induce overproduction of reactive oxygen species (ROS) and collapse of mitochondrial membrane potential, leading to cell damage. Moreover, ZnO nanoparticles also significantly reduced expression of MnSOD at both the mRNA and protein levels, reduced its activity, and further aggravated oxidative stress-mediated cell damage. Conclusion Overall, ZnO nanoparticle-induced cytotoxicity was associated with elevated levels of oxidative stress due to overproduction of ROS and reduced expression and activity of MnSOD.