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Increased systemic RNA oxidative damage and diagnostic value of RNA oxidative metabolites during Shigella flexneri-induced intestinal infection

Yttrium stabilized zirconia (YSZ) used as the state-of-the-art electrolyte for solid oxide fuel cells (SOFCs) requires high temperature (over 800 °C) to realize sufficient oxygen ion conductivity. Thus, the high operational temperature is the main restriction for the commercial process of YSZ-based SOFCs. To obtain decent ionic conductivity at intermediate-low temperatures, Sr-free cathode LaNiO3 is introduced into YSZ to construct a novel LaNiO3-YSZ composite electrolyte, which is sandwiched by two Ni0.8Co0.15Al0.05LiO2-δ (NCAL) electrodes to assemble systematical fuel cells. This device presents an excellent peak output of 1045 mW cm-2 at 600 °C and even 399 mW cm-2 at 450 °C. A series of characterizations indicates that the oxygen ion conductivity of the LaNiO3-YSZ composite is significantly promoted in comparison with that of pure YSZ, and the LaNiO3 component has certain proton conductivity after hydrogenation. Both of the two factors contributes to the superior performance of such devices at intermediate-low temperatures. Furthermore, the sharp decrease in electronic conductivity for LaNiO3 in hydrogen atmosphere combined with Schottky junction at the anode-electrolyte interface eliminates the short-circuiting problem. Our work demonstrates that incorporating Sr-free cathode LaNiO3 into the YSZ electrolyte is an efficient strategy to boost the performance and reduce the operational temperature of YSZ-based SOFCs.

Recently, due to the unique advantage in ionic conductivity, semiconductor–ionic conductor composite (SIC) has been widely applied as electrolyte for solid oxide fuel cell (SOFC), and the suitable anode and cathode should be chosen to match the SIC electrolyte. Inspired by this ideology, the semiconductor lanthanum nitrate (LaNiO3) with perovskite structure was prepared by sol-gel method and then composed with ionic conductor yttrium-stabilized zirconia (YSZ) to form LaNiO3-YSZ SIC, which was applied as electrolyte membrane, simultaneously the Li2CO3–LaNiO3 composite and Ni0.8Co0.15Al0.05LiO2 (NCAL) were respectively utilized as anode and cathode for fuel cell construction. Through optimizing the electrolyte composition, such device based on LaNiO3-YSZ electrolyte and Li2CO3–LaNiO3 anode received a supreme performance of 505 mW/cm2 at 550 °C, and the maximum power density can also maintain at 350 mW/cm2 even as the temperature dropped to 475 °C. The excellent performance is attributed to the decent ionic conductivity of LaNiO3-YSZ electrolyte and the outstanding catalysis activity of electrodes. In addition, we have found that the addition of Li2CO3 component into anode can substantially enhance the cell performance. Our result demonstrates that the multifunctional LaNiO3 can be simultaneously applied as electrolyte and anode for low-temperature SOFC.

As a prominent cryptographic tool, geometric searchable encryption (GSE) can be applied in many scenarios, such as location-based services (LBS), social networks and vehicle networks. Unfortunately, most of existing searchable encryption schemes supporting the functionality of geometric range searches suffer from false positives, which will lead people to make a wrong decision and further raise some serious consequences such as financial loss. In addition, some of them are designed under a symmetric system, which is not enough flexible deployed in LBS since in a symmetric system only a private key holder creates ciphertext, whereas in a public-key system anyone who holds a public key can produce ciphertext. In this paper, we intend to design a novel GSE scheme without any false positive under a public-key system supporting arbitrary geometric area searches, which is able to guarantee an accurate query result. Toward this goal, we develop a novel technique in handling the relation between a point and any convex polygon in combination with an inner product encryption, which is able to support arbitrary convex polygon range searches without any false positive. A comprehensive experiment demonstrates that, compared with the known schemes, our scheme possesses a 100% accuracy as well as an acceptable efficiency in the sense that it can guarantee that all files retrieved by users are exactly matched ones. Finally, we provide two practical examples of our GSE scheme: privacy-preserving friend-nearby notification with a common point of interest and privacy-preserving parking monitor and guiding system.

The emergence of Internet technology has changed the technological innovation path and knowledge acquisition paradigm of enterprises and promoted the construction of digital technology, digital platform, and digital infrastructure. A large number of companies have begun to create brand communities and obtain suggestions for product improvement and innovation from the communities. However, obtaining innovative opinions and inspiration through the community to the final realization is not achieved overnight. It needs to go through the process of knowledge sharing to user interaction, from user interaction to knowledge creation and discovery and from knowledge discovery to innovation contribution. To study the efficiency of Internet community innovation, it is necessary to decompose the innovation process into two parts: problem adoption and problem solution, and then analyze them separately to get the influence combination and path. Taking Xiaomi (one of the world’s largest mobile phone manufacturers) online community as an example, this article decomposes the process of issue solution into adoption stage and solution stage by crawling the relevant data of posts through web crawlers, takes the adoption efficiency and problem-solving efficiency as antecedents, and uses fuzzy set qualitative comparative analysis method to analyze the combination of conditions that affect the former two. It is of great significance to improve the knowledge sharing and innovation research of the open innovation community.

A series of linear amphiphilic pentablock terpolymer PAAx-b-PS48-b-PEO46-b-PS48-b-PAAx (AxS48O46S48Ax) with various lengths x of the PAA block (x = 15, 40, 60, and 90) were synthesized via a two-step atom transfer radical polymerization (ATRP) using Br-poly(ethylene oxide)-Br (Br-PEO46-Br) as the macroinitiator, styrene (St) as the first monomer, and tert-butyl acrylate (tBA) as the second monomer, followed with the hydrolysis of PtBA blocks. The AxS48O46S48Ax pentablock terpolymers formed micelles in dilute aqueous solution, of which the morphologies were dependent on the length x of the PAA block. Cryogenic transmission electron microscopy (cryo-TEM), dynamic light scattering (DLS), and zeta potential measurement were employed to investigate the morphologies, chain structures, size, and size distribution of the obtained micelles. The morphology of AxS48O46S48Ax micelles changed from spherical vesicles with ordered porous membranes to long double nanotubes, then to long nanotubes with inner modulated nanotubes or short nanotubes, and finally, to spherical micelles or large compound vesicles with spherical micelles inside when x increased from 15 to 90. The hydrophobic PS blocks formed the walls of vesicles and nanotubes as well as the core of spherical micelles. The hydrophilic PEO and PAA block chains were located on the surfaces of vesicle membranes, nanotubes, and spherical micelles. The PAA block chains were partially ionized, leading to the negative zeta potential of AxS48O46S48Ax micelles in dilute aqueous solutions.

Objective: To explore the relationship between miR-7-5p and brain edema after intracerebral hemorrhage and the role of butylphthalide (NBP) in brain edema after intracerebral hemorrhage.Method: Routine blood testing, C-reactive protein results, and computed tomography data were collected 1, 7, and 14 days after intracerebral hemorrhage in six patients. Levels of MMP-9, ZO-1, occludin, IL-6, TNF-α, and miR-7-5p were detected in each patient's serum. Sixty male Sprague–Dawley rats were randomly divided into sham operation, intracerebral hemorrhage, and NBP treatment groups. Dry–wet weight was used to assess brain edema, and Evans blue staining was used to assess the permeability of the blood–brain barrier. Expression levels of IL-6, TNF-α, ZO-1 and occludin, PI3K, AKT, p-AKT, AQP4, and miR-7-5p were analyzed in the rat brains.Result: The blood neutrophil–lymphocyte ratio (NLR) on day 1 was associated with the area of brain edema on day 7. The expression of miR-7-5p decreased after intracerebral hemorrhage, and as a result, the inhibition of the PI3K/AKT pathway was weakened. The decreased inhibition of the PI3K/AKT pathway resulted in an increase in AQP4 expression, which further aggravated brain edema. NBP can upregulate the expression of miR-7-5p, affecting these pathways to reduce brain edema.Conclusion: After intracerebral hemorrhage, miR-7-5p expression in brain tissue is reduced, which may increase the expression of AQP4 by activating the PI3K/AKT pathway. NBP can inhibit this process and reduce brain edema.

The sensitivity and response time of glutamate sensors based on glutamate oxidase immobilized on planar platinum microelectrodes have been improved to near the theoretical performance limits predicted by a detailed mathematical model. Microprobes with an array of electroenzymatic sensing sites have emerged as useful tools for the monitoring of glutamate and other neurotransmitters in vivo; and implemented as such, they can be used to study many complex neurological diseases and disorders including Parkinson’s disease and drug addiction. However, less than optimal sensitivity and response time has limited the spatiotemporal resolution of these promising research tools. A mathematical model has guided systematic improvement of an electroenzymatic glutamate microsensor constructed with a 1-2 μm-thick crosslinked glutamate oxidase layer and underlying permselective coating of polyphenylenediamine and Nafion reduced to less than 200 nm thick. These design modifications led to a nearly 6-fold improvement in sensitivity to 320 ± 20 nA μM(−1) cm(−2) at 37 °C and a ~10-fold reduction in response time to 80 ± 10 ms. Importantly, the sensitivity and response times were attained while maintaining a low detection limit and excellent selectivity. Direct measurement of the transport properties of the enzyme and polymer layers used to create the biosensors enabled improvement of the mathematical model as well. Subsequent model simulations indicated that the performance characteristics achieved with the optimized biosensors approach the theoretical limits predicted for devices of this construction. Such high-performance glutamate biosensors will be more effective in vivo at a size closer to cellular dimension and will enable better correlation of glutamate signaling events with electrical recordings.

Microgels with a thermo-sensitive poly(N-isopropylacrylamide) (polyNIPAm) backbone and bis-imidazolium (VIM) ionic cross-links, denoted as poly(NIPAm-co-VIM), were successfully prepared. The as-synthesized ionic microgels were converted to nanoreactors, denoted as Au@PNI MGs, upon generation and immobilization of gold nanoparticles (Au NPs) of 5–8 nm in size into poly(NIPAm-co-VIM). The content of Au NPs in microgels could be regulated by controlling the 1,6-dibromohexane/vinylimidazole molar ratio in the quaternization reaction. The microgel-based nanoreactors were morphologically spherical and uniform in size, and presented reversible thermo-sensitive behavior with volume phase transition temperatures (VPTTs) at 39–40 °C. The Au@PNI MGs were used for the reduction of 4-nitrophenol, of which the catalytic activity could be modulated by temperature.

This is a post-peer-review, pre-copyedit version of an article published in Microchimica Acta. The final authenticated version is available online at: 10.1007/s00604-017-2568-6 &nbsp

We synthesized a series of poly(N-isopropylacrylamide)-based ionic hydrogels with dicationic ionic liquid crosslinking structures. The obtained ionic hydrogels showed typical feature of poly(ionic liquid) in aqueous solutions, of which the quaternized imidazolium moieties could interfacially interact with anionic dyes, such as methyl orange (MO), methyl blue (MB), congo red (CR), orange G (OG), thymol blue (TB) and bromothymol blue (BTB), which were strongly dependent on the chemical structures of the dyes.

The novel microgels, poly[di(ethylene glycol) methyl ether methacrylate-co-2-methoxyethyl acrylate] poly(DEGMMA-co-MEA) microgels, were synthesized. The poly(DEGMMA-co-MEA) microgels were thermo-sensitive and exhibited a volume phase transitive temperature (VPTT) of 14–22 °C. The incorporation of hydrophobic comonomer MEA shifted the VPTT of poly(DEGMMA-co-MEA) microgels to lower temperatures. The interfacial interaction of poly(DEGMMA-co-MEA) microgels and three model proteins, namely fibrinogen, bovine serum albumin and lysozyme, was investigated by quartz crystal microbalance (QCM). An injection sequence of “microgel-after-protein” was then established for the real-time study of the interaction of proteins and the microgels at their swollen and collapsed states by using QCM technique. The results indicated that the interfacial interaction of poly(DEGMMA-co-MEA) microgels and adsorbed protein layers was mainly determined by the electrostatic interaction. Because poly(DEGMMA-co-MEA) microgels were negatively charged in Tris-HCl buffer solution (pH = 7.4), the microgels did not adsorb on negatively charged fibrinogen and bovine serum albumin layers but strongly adsorbed on positively charged lysozyme layer. Stronger interaction between lysozyme and the microgels at collapsed state (i.e. at 37 °C) was observed. Furthermore, the incorporation of MEA might weaken the interaction between poly(DEGMMA-co-MEA) microgels and proteins.

A magnetic bead-based SELEX was applied to identify 37 single-stranded DNA aptamers specific for tobramycin after ten rounds of selection. The aptamers were classified into nine families according to sequence analysis. Among them, several aptamers with typical sequences were selected and their dissociation constants (Kds) were determined by a fluorescent method. An aptamer termed “Ap 32”, with a Kd value of 56.8 ± 4.6 nM, possesses the highest affinity and satisfactory specificity. Theoretical modeling showed that nucleotides 14–18 and 26–29 play a most significant role in the interaction between aptamer and tobramycin. Subsequently, the sequence of Ap 32 was optimized through rationally designed truncation. The truncated aptamer Ap 32–2 consists of 34 nucleotides and has a Kd that is similar to the original one. It was chosen as the optimal aptamer for use in the assay and was immobilized on gold nanoparticles. On addition of tobramycin, the color turns from red to purple. The findings were used to design a photometric assay (best performed at 520 nm) that has a linear response in the 100 nM to 1.4 μM concentration range, with a 37.9 nM detection limit. The method was successfully applied to the determination of tobramycin in (spiked) honey samples.

A highly selective and sensitive optical sensor was developed to colorimetric detect trace Fe3+ ions in aqueous solution. The sensor was the sulfasalazine (SSZ) functionalized microgels (SSZ-MGs), which were fabricated via in-situ quaternization reaction. The obtained SSZ-MGs had hydrodynamic radius of about 259 &plusmn, 24 nm with uniform size distribution at 25 &deg, C. The SSZ-MG aqueous suspensions can selectively and sensitively response to Fe3+ ions in aqueous solution at 25 &deg, C and pH of 5.6, which can be quantified by UV-visible spectroscopy and also easily distinguished by the naked eye. Job&rsquo, s plot indicated that the molar binding ratio of SSZ moiety in SSZ-MGs to Fe3+ was close to 1:1 with an apparent association constant of 1.72 &times, 104 M&minus, 1. A linear range of 0&ndash, 12 &mu, M with the detection limit of 0.110 &mu, M (0.006 mg/L) was found. The obtained detection limit was much lower than the maximum allowance level of Fe3+ ions in drinking water (0.3 mg/L) regulated by the Environmental Protection Agency (EPA) of the United States. The existence of 19 other species of metal ions, namely, Ag+, Li+, Na+, K+, Ca2+, Ba2+, Cu2+, Ni2+, Mn2+, Pb2+, Zn2+, Cd2+, Co2+, Cr3+, Yb3+, La3+, Gd3+, Ce3+, and Bi3+, did not interfere with the detection of Fe3+ ions.

Self-diffusion and structural properties of n-alkanes have been studied by molecular dynamics simulation in the temperature range between the melting pressure curve and 600 K at pressures up to 300 MPa. The simulated results of lower n-alkanes are in good agreement with the existing experimental data, and support the reliability of results of the simulations of self-diffusion coefficients obtained at the extreme conditions. We predict the self-diffusion coefficients for methane, ethane, propane and n-butane at the similar reduced temperatures and pressures to draw a comparison between them. Then the correlation between self-diffusion and structural properties are further investigated by calculating the coordination numbers. Moreover, we define four distances and their corresponding relative deviations to characterize the flexibility of long-chain n-alkanes. The simulated results show that the self-diffusion of n-alkane molecules is mainly affected by the close packing, and the flexibility has a strong impact on the self-diffusion of longer n-alkane molecules.

Utilizing the hydrolysis and condensation of the methoxysilyl moieties, organic-inorganic hybrid poly(N-isopropylacrylamide-co-acrylamide-co-3-(trimethoxysilyl)propylmethacrylate) P(NIPAM-co-AM-co-TMSPMA) microgels were prepared via two different methods. The first method was that the microgels were post-fabricated from the crosslinkable linear P(NIPAM-co-AM-co-TMSPMA) terpolymer aqueous solutions above the lower critical solution temperature (LCST) of the terpolymer. For the second method, the microgels were directly synthesized by conventional surfactant free emulsion copolymerization of NIPAM, AM, and TMSPMA. The hydrodynamic diameter and stability of the resultant P(NIPAM-co-AM-co-TMSPMA) microgels strongly depend on the pH and temperature of the microgel aqueous solution. The hydrodynamic diameters of the microgels decreased with increasing the measuring temperature. The phase transition temperature of the microgels was found to be around 34°C, which was independent of the initial terpolymer concentration and shifted to lower temperature with increasing the preparation temperature. Increasing the initial amount of AM will enhance the instability of the microgels at high pH values. Moreover, the P(NIPAM-co-AM-co-TMSPMA) microgels obtained from the linear terpolymer had more homogeneous microstructures as compared with the corresponding NIPAM/AM/TMSPMA microgels prepared by one step emulsion copolymerization as revealed by light scattering measurements.

Silver nanoparticles (Ag NPs) stabilized by a thermoresponsive polymer, poly(N-isopropylacrylamide) (PNIPAM), have been synthesized by the reduction of silver ions with NaBH(4) in aqueous solutions. The obtained Ag NPs are very stable at room temperature due to the extended coil conformation of the PNIPAM chain at temperatures below its volume phase transition temperature ( approximately 32 degrees C). At higher temperatures (such as 45 degrees C) above the phase transition of PNIPAM, only minute aggregation between Ag NPs was observed, showing that the collapsed PNIPAM chains still retain the ability to stabilize Ag NPs. The PNIPAM-stabilized Ag NPs were then characterized as a function of the thermal phase transition of PNIPAM by UV-vis spectroscopy, dynamic light scattering, transmission electron microscopy, and cyclic voltammeter. Consistent results were obtained showing that the phase transition of PNIPAM has some effect on the optical properties of Ag NPs. Switchable electrochemical response of the PNIPAM-stabilized Ag NPs triggered by temperature change was observed.

The contributions of the dynamic fluctuations and the frozen-in inhomogeneities to the total light scattering intensity observed in poly(N-isopropylacrylamide)/clay nanocomposite hydrogels were analyzed by applying the nonergodic method proposed by Pusey and van Megen. Approximately 90% of the total scattering intensity corresponds to the frozen-in component. The scattering intensity of the fluctuating component is smaller by far than that of a pure clay suspension, indicating that the thermal fluctuations of the clay particles are largely suppressed upon network formation. Accordingly, the fluctuating component consists of two contributions: one due to the polymer chains and the other, smaller one representing the residual mobility of the clay particles. The latter depends on how tightly the clay particles are fixed in the network. The dynamic features of the nanocomposite hydrogels are described by two relaxation modes. The fast one is purely diffusive and can be related to a dynamic correlation length of 6-8 nm, which is similar to that of a corresponding polymer solution. The relaxation time of the slow mode varies appreciably with sample position even though the data had been treated with the nonergodic method.