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Little is known about the ecology of the Indo-Pacific finless porpoise Neophocaena phocaenoides or the Indo-Pacific humpback dolphin Sousa chinensis in Southeast Asia. The present study describes the distribution and habitat preferences of these species around the Langkawi Archipelago of Malaysia. Vessel-based passive acoustic monitoring surveys were conducted 5 times between 2012 and 2013. Both species mainly preferred relatively shallow waters, especially on the east sides of the islands at

Background: Animal-borne sensors ('bio-loggers') can record a suite of kinematic and environmental data, which are used to elucidate animal ecophysiology and improve conservation efforts. Machine learning techniques are used for interpreting the large amounts of data recorded by bio-loggers, but there exists no common framework for comparing the different machine learning techniques in this domain. This makes it difficult to, for example, identify patterns in what works well for machine learning-based analysis of bio-logger data. It also makes it difficult to evaluate the effectiveness of novel methods developed by the machine learning community., Methods: To address this, we present the Bio-logger Ethogram Benchmark (BEBE), a collection of datasets with behavioral annotations, as well as a modeling task and evaluation metrics. BEBE is to date the largest, most taxonomically diverse, publicly available benchmark of this type, and includes 1654 h of data collected from 149 individuals across nine taxa. Using BEBE, we compare the performance of deep and classical machine learning methods for identifying animal behaviors based on bio-logger data. As an example usage of BEBE, we test an approach based on self-supervised learning. To apply this approach to animal behavior classification, we adapt a deep neural network pre-trained with 700,000 h of data collected from human wrist-worn accelerometers., Results: We find that deep neural networks out-perform the classical machine learning methods we tested across all nine datasets in BEBE. We additionally find that the approach based on self-supervised learning out-performs the alternatives we tested, especially in settings when there is a low amount of training data available., Conclusions: In light of these results, we are able to make concrete suggestions for designing studies that rely on machine learning to infer behavior from bio-logger data. Therefore, we expect that BEBE will be useful for making similar suggestions in the future, as additional hypotheses about machine learning techniques are tested. Datasets, models, and evaluation code are made publicly available at https://github.com/earthspecies/BEBE , to enable community use of BEBE., Competing Interests: Declarations. Ethics approval and consent to participate: All animal behavior datasets except the Crow dataset were reported in previous publications. Crow behavior data were collected in accordance with ASAB/ABS guidelines and Spanish regulations for animal research, and were authorized by Junta de Castilla y León (licence: EP/LE/681-2019). Competing interest: The authors declare no competing interests., (© 2024. The Author(s).)

Northern elephant seals (Mirounga angustirostris) have been integral to the development and progress of biologging technology and movement data analysis, which continue to improve our understanding of this and other species. Adult female elephant seals at Año Nuevo Reserve and other colonies along the west coast of North America were tracked annually from 2004 to 2020, resulting in a total of 653 instrument deployments. This paper outlines the compilation and curation process of these high-resolution diving and location data, now accessible in two Dryad repositories. The code used for data processing alongside the corresponding workflow is available through GitHub and Zenodo. This data set represents 3,844,927 dives and 596,815 locations collected from 475 individual seals with 178 repeat samplings over 17 years. We anticipate that these data will stimulate further analysis and investigation into elephant seal biology and aid in developing new analytical approaches for large marine predators., Competing Interests: Competing interests: The authors declare no competing interests., (© 2024. The Author(s).)

Previous studies have described two distinct vascular systems in cetacean fins. However, these studies have been limited to Delphinoidea species, with little information on their three-dimensional structures. In this study, the anatomical analysis of the caudal and dorsal fins of a dwarf sperm whale was conducted using X-ray computed tomography and gross dissection with staining, providing the first confirmation of the two vascular systems in the fins of the family Kogiidae. This finding suggested that these vascular systems are common across Odontoceti species. In addition, we observed unique three-dimensional structures in the superficial veins, which formed a reticulate pattern in both fins and converged at the centerline at the base of the dorsal fin, potentially influencing individual's heat reduction capabilities., Competing Interests: The authors declare no conflicts of interest., (© 2024 The Author(s). Ecology and Evolution published by John Wiley & Sons Ltd.)

We developed the "Itappachi" universal motion platform for measuring radiation doses under simulated respiratory motion in radiation therapy. The interplay effect, resulting from respiratory motion, degrades dose delivery precision in advanced treatments such as volumetric modulated arc therapy. The Itappachi platform is designed for precise dose measurement in dynamic scenarios through its ability to simulate respiratory motion. The platform features a large surface (580 mm × 380 mm), capable of supporting weights up to 56.8 kg, and moves with an amplitude of ±25 mm. It requires no silicone oil for maintenance, and it is controlled via Wi-Fi using user-owned devices, thereby reducing costs. Its motion accuracy was confirmed, with a maximum displacement error of 0.12 mm. Using the platform, we conducted dose measurements with a Delta4 Phantom under static, moving without gated irradiation, and moving with gated irradiation conditions. Gamma index analysis revealed excellent agreement for static and dynamic gated conditions (99.4%), while significant dose degradation occurred in the non-gated dynamic condition (32.6%). The Itappachi platform provides a cost-effective and accurate solution for dose measurement under respiratory motion by which it supports improved quality assurance in radiation therapy., Competing Interests: Human subjects: All authors have confirmed that this study did not involve human participants or tissue. Animal subjects: All authors have confirmed that this study did not involve animal subjects or tissue. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: Dr. Shinichiro Mori and Mr. Kazuhide Uchida are employed by Perfect Imaging Laboratory Inc., Chiba, Japan. Dr. Kiyoshi Yoda is employed by Anzai Medical Co., Ltd., Tokyo, Japan. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Intellectual property info: The relevant patent is submitted to the Japanese Patent. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work., (Copyright © 2024, Tohyama et al.)

The role of the gut microbiota in the gut-brain axis has attracted attention in recent years. Some gut microbiota produces γ-aminobutyric acid (GABA), a major inhibitory neurotransmitter in mammals, in vitro , but the correlation between gut microbiota composition and intestinal GABA concentration, as well as the action of intestinal GABA in vivo , are poorly understood. Herein, we found that the intestinal GABA concentration was increased in mice by the intervention of the gut microbiota with neomycin or Bifidobacterium bifidum TMC3115 (TMC3115). Administration of TMC3115 reduced anxiety without affecting serum levels of serotonin, corticosterone, or GABA. We further found that intestinal epithelial cells expressed GABA receptor subunits and mediated mitogen-activated protein kinase signaling upon GABA stimulation. In addition, administration of TMC3115 induced mitogen-activated protein kinase signaling in colonic epithelial cells but not in small intestinal epithelial cells in mice. These results indicate that GABA produced by the gut microbiota, mainly in the colon, may affect host behavioral characteristics via GABA receptors expressed in intestinal epithelial cells without being transferred to the blood. This study suggests a novel mechanism by which intestinal GABA exerts physiological effects, even in the presence of the blood-brain barrier., Competing Interests: GH, KY, and KM are employees of Takanashi Milk Products Co., Ltd. Kanagawa, Japan. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Ikegami, Narabayashi, Nakata, Yamashita, Sugi, Fuji, Matsufuji, Harata, Yoda, Miyazawa, Nakanishi and Takahashi.)

Research on predator-prey interactions between sharks and cetaceans remain limited. Here, we report on a video of a shortfin mako shark circling a finless porpoise with a damaged caudal fin in the Seto Inland Sea, Japan. The finless porpoise was neither emaciated nor inactive, but unable to swim effectively due to the complete lack of a caudal fin. Some circumstantial evidence, including a bite mark on the porpoise's head, strongly suggests that the mako shark attacked it. Furthermore, the possible time difference between the two injuries the porpoise sustained may reflect the shark's hunting tactics. While mako sharks primarily feed on small fish and cephalopods, this observation suggests they also may prey on live cetaceans more often than previously thought., Competing Interests: The authors have no conflict of interests to declare that are relevant to the content of this article., (© 2024 The Author(s). Ecology and Evolution published by John Wiley & Sons Ltd.)

The way goal-oriented birds adjust their travel direction and route in response to wind significantly affects their travel costs. This is expected to be particularly pronounced in pelagic seabirds, which utilize a wind-dependent flight style called dynamic soaring. Dynamic soaring seabirds in situations without a definite goal, e.g. searching for prey, are known to preferentially fly with crosswinds or quartering-tailwinds to increase the speed and search area, and reduce travel costs. However, little is known about their reaction to wind when heading to a definite goal, such as homing. Homing tracks of wandering albatrosses ( Diomedea exulans ) vary from beelines to zigzags, which are similar to those of sailboats. Here, given that both albatrosses and sailboats travel slower in headwinds and tailwinds, we tested whether the time-minimizing strategies used by yacht racers can be compared to the locomotion patterns of wandering albatrosses. We predicted that when the goal is located upwind or downwind, albatrosses should deviate their travel directions from the goal on the mesoscale and increase the number of turns on the macroscale. Both hypotheses were supported by track data from albatrosses and racing yachts in the Southern Ocean confirming that albatrosses qualitatively employ the same strategy as yacht racers. Nevertheless, albatrosses did not strictly minimize their travel time, likely making their flight robust against wind fluctuations to reduce flight costs. Our study provides empirical evidence of tacking in albatrosses and demonstrates that man-made movement strategies provide a new perspective on the laws underlying wildlife movement., Competing Interests: Competing interests statement:The authors declare no competing interest.

Functional foods with probiotics are safe and effective dietary supplements to improve overweight and obesity. Thus, altering the intestinal microflora may be an effective approach for controlling or preventing obesity. This review aims to summarize the experimental method used to study probiotics and obesity, and recent advances in probiotics against obesity. In particular, we focused on studies (in vitro and in vivo) that used probiotics to treat obesity and its associated comorbidities. Several in vitro and in vivo (animal and human clinical) studies conducted with different bacterial species/strains have reported that probiotics promote anti-obesity effects by suppressing the differentiation of pre-adipocytes through immune cell activation, maintaining the Th1/Th2 cytokine balance, altering the intestinal microbiota composition, reducing the lipid profile, and regulating energy metabolism. Most studies on probiotics and obesity have shown that probiotics are responsible for a notable reduction in weight gain and body mass index. It also increases the levels of anti-inflammatory adipokines and decreases those of pro-inflammatory adipokines in the blood, which are responsible for the regulation of glucose and fatty acid breakdown. Furthermore, probiotics effectively increase insulin sensitivity and decrease systemic inflammation. Taken together, the intestinal microbiota profile found in overweight individuals can be modified by probiotic supplementation which can create a promising environment for weight loss along enhancing levels of adiponectin and decreasing leptin, tumor necrosis factor (TNF)-α, interleukin (IL)-6, monocyte chemotactic protein (MCP)-1, and transforming growth factor (TGF)-β on human health.

Waste beverages are utilized as resources in various valuable, albeit energy-consuming, waste-to-energy processes. There is a growing need for alternative cost-effective methods to harness their potential. This study explored the feasibility of employing waste beverages as feedstock for the counterpart component of a TiO 2 -based composite photocatalyst. Several commonly available carbonated soft drinks from the Japanese market have been investigated to achieve this goal. The investigation revealed that a mild hydrothermal treatment condition could transform all examined beverages into carbonaceous materials suitable for fabricating a core-shell structure with TiO 2 , resulting in a remarkably efficient visible light active photocatalyst. Notably, a pH-adjusted photocatalyst derived from Coca Cola® exhibited superior visible light photodegradability toward dye molecules and enhanced bactericidal efficacy compared to the counterpart derived from pure sucrose. The heightened visible light photocatalytic activity can be attributed to the distinctive carboxy-rich surface functional groups, based on the findings of experimental analyses and density functional theory calculations. The bidentate-type bonding of these groups with TiO 2 induces a modified interfacial bond structure that facilitates the efficient transfer of photoexcited carriers. This study presents a novel avenue for the effective utilization and recycling of waste beverages, and adds value under environmentally benign conditions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Rare behaviors displayed by wild animals can generate new hypotheses; however, observing such behaviors may be challenging. While recent technological advancements, such as bio-loggers, may assist in documenting rare behaviors, the limited running time of battery-powered bio-loggers is insufficient to record rare behaviors when employing high-cost sensors (e.g. video cameras). In this study, we propose an artificial intelligence (AI)-enabled bio-logger that automatically detects outlier readings from always-on low-cost sensors, e.g. accelerometers, indicative of rare behaviors in target animals, without supervision by researchers, subsequently activating high-cost sensors to record only these behaviors. We implemented an on-board outlier detector via knowledge distillation by building a lightweight outlier classifier supervised by a high-cost outlier behavior detector trained in an unsupervised manner. The efficacy of AI bio-loggers has been demonstrated on seabirds, where videos and sensor data captured by the bio-loggers have enabled the identification of some rare behaviors, facilitating analyses of their frequency, and potential factors underlying these behaviors. This approach offers a means of documenting previously overlooked rare behaviors, augmenting our understanding of animal behavior., (© The Author(s) 2024. Published by Oxford University Press on behalf of National Academy of Sciences.)

Plastic pollution is distributed patchily around the world's oceans. Likewise, marine organisms that are vulnerable to plastic ingestion or entanglement have uneven distributions. Understanding where wildlife encounters plastic is crucial for targeting research and mitigation. Oceanic seabirds, particularly petrels, frequently ingest plastic, are highly threatened, and cover vast distances during foraging and migration. However, the spatial overlap between petrels and plastics is poorly understood. Here we combine marine plastic density estimates with individual movement data for 7137 birds of 77 petrel species to estimate relative exposure risk. We identify high exposure risk areas in the Mediterranean and Black seas, and the northeast Pacific, northwest Pacific, South Atlantic and southwest Indian oceans. Plastic exposure risk varies greatly among species and populations, and between breeding and non-breeding seasons. Exposure risk is disproportionately high for Threatened species. Outside the Mediterranean and Black seas, exposure risk is highest in the high seas and Exclusive Economic Zones (EEZs) of the USA, Japan, and the UK. Birds generally had higher plastic exposure risk outside the EEZ of the country where they breed. We identify conservation and research priorities, and highlight that international collaboration is key to addressing the impacts of marine plastic on wide-ranging species., (© 2023. The Author(s).)

Background and aim Magnetic resonance (MR) imaging has been increasingly adopted in the field of radiotherapy, and the most advanced MR image-guided radiotherapy is known as MR-guided online adaptive radiotherapy (MRgOART), which integrates MRI and linac systems. Few attempts have yet been made to directly compare treatment outcomes between the MRgOART and standard computed tomography (CT)-guided radiotherapy (CTgRT). Besides, it is reported that the biologically equivalent dose (BED) may be a good predictor of the local control (LC) and the overall survival (OS) for liver tumors. The purpose of this study is to compare the BEDs between the MRgOART and the CTgRT by way of virtual isotoxic planning for liver tumors. The hypothesis of this study is therefore that the MRgOART increases LC and OS as compared to the CTgRT. Materials and methods Using the five patient cases available, isotoxic planning was performed. For CTgRT, an internal target volume (ITV) was defined, and the planning target volume (PTV) was created by adding an isotropic margin of 10 mm. For MRgRT, a gross tumor volume (GTV) was defined, and the PTV was created by adding an isotropic margin of 5 mm. Each tumor size was virtually adjusted so that the CTgRT plans resulted in BED <100 Gy under the condition that the nearest organs at risk receive maximum tolerated doses. Subsequently, the BED was recalculated for MRgOART plans with the adjusted tumor size. Results and discussion It was found that the BEDs of the MRgOART plans always exceeded 100 Gy and were approximately 20 Gy larger than those of the corresponding CTgRT plans. Literature shows that superior overall survival rates for liver tumors were observed when BED was >100 Gy as compared to BED <100 Gy, suggesting that MR-guided adaptive planning may potentially lead to better treatment outcomes for liver tumors. We have also observed a case where the duodenum largely moved and abutted the liver after the CT images were acquired, indicating a significant disadvantage of the standard CTgRT because such abutting is not observable by the cone-beam CT immediately before treatment. Conclusion A highly accelerated evidence-creation procedure to suggest the clinical superiority of MRgOART has been arguably proposed with promising results. The sample size is small and limits the extent to which the findings in this study can be generalized. Further virtual clinical trials within the radiotherapy community are awaited with more clinical outcomes data., Competing Interests: The authors have declared financial relationships, which are detailed in the next section., (Copyright © 2023, Yoda et al.)

Cyclones can cause mass mortality of seabirds, sometimes wrecking thousands of individuals. The few studies to track pelagic seabirds during cyclones show they tend to circumnavigate the strongest winds. We tracked adult shearwaters in the Sea of Japan over 11 y and found that the response to cyclones varied according to the wind speed and direction. In strong winds, birds that were sandwiched between the storm and mainland Japan flew away from land and toward the eye of the storm, flying within ≤30 km of the eye and tracking it for up to 8 h. This exposed shearwaters to some of the highest wind speeds near the eye wall (≤21 m s -1 ) but enabled them to avoid strong onshore winds in the storm's wake. Extreme winds may therefore become a threat when an inability to compensate for drift could lead to forced landings and collisions. Birds may need to know where land is in order to avoid it. This provides additional selective pressure for a map sense and could explain why juvenile shearwaters, which lack a map sense, instead navigating using a compass heading, are susceptible to being wrecked. We suggest that the ability to respond to storms is influenced by both flight and navigational capacities. This may become increasingly pertinent due to changes in extreme weather patterns.

Body-mounted accelerometers provide a new prospect for estimating power use in flying birds, as the signal varies with the two major kinematic determinants of aerodynamic power: wingbeat frequency and amplitude. Yet wingbeat frequency is sometimes used as a proxy for power output in isolation. There is, therefore, a need to understand which kinematic parameter birds vary and whether this is predicted by flight mode (e.g. accelerating, ascending/descending flight), speed or morphology. We investigate this using high-frequency acceleration data from (i) 14 species flying in the wild, (ii) two species flying in controlled conditions in a wind tunnel and (iii) a review of experimental and field studies. While wingbeat frequency and amplitude were positively correlated, R 2 values were generally low, supporting the idea that parameters can vary independently. Indeed, birds were more likely to modulate wingbeat amplitude for more energy-demanding flight modes, including climbing and take-off. Nonetheless, the striking variability, even within species and flight types, highlights the complexity of describing the kinematic relationships, which appear sensitive to both the biological and physical context. Notwithstanding this, acceleration metrics that incorporate both kinematic parameters should be more robust proxies for power than wingbeat frequency alone.

The largest extinct volant birds ( Pelagornis sandersi and Argentavis magnificens ) and pterosaurs ( Pteranodon and Quetzalcoatlus ) are thought to have used wind-dependent soaring flight, similar to modern large birds. There are 2 types of soaring: thermal soaring, used by condors and frigatebirds, which involves the use of updrafts to ascend and then glide horizontally; and dynamic soaring, used by albatrosses, which involves the use of wind speed differences with height above the sea surface. Previous studies have suggested that P. sandersi used dynamic soaring, while A. magnificens and Quetzalcoatlus used thermal soaring. For Pteranodon , there is debate over whether they used dynamic or thermal soaring. However, the performance and wind speed requirements of dynamic and thermal soaring for these species have not yet been quantified comprehensively. We quantified these values using aerodynamic models and compared them with that of extant birds. For dynamic soaring, we quantified maximum travel speeds and maximum upwind speeds. For thermal soaring, we quantified the animal's sinking speed circling at a given radius and how far it could glide losing a given height. Our results confirmed those from previous studies that A. magnificens and Pteranodon used thermal soaring. Conversely, the results for P. sandersi and Quetzalcoatlus were contrary to those from previous studies. P. sandersi used thermal soaring, and Quetzalcoatlus had a poor ability both in dynamic and thermal soaring. Our results demonstrate the need for comprehensive assessments of performance and required wind conditions when estimating soaring styles of extinct flying species., (© The Author(s) 2022. Published by Oxford University Press on behalf of the National Academy of Sciences.)

With the accumulation of knowledge on the relation between psychological stress and gut microbiota, there is growing interest in controlling stress and/or mood disorders via probiotic supplementation. We aimed to examine the effect of probiotic Bifidobacterium bifidum TMC3115 (TMC3115) supplementation using a sub-chronic and mild social defeat stress murine model in this study. TM3115 supplementation maintained body weight gain and alleviated a polydipsia-like symptom induced by the stress. In the analyses of fecal and cecal bacterial profiles, expansions of Proteobacteria in stressed mice and increases in Actinobacteria and Bifidobacterium in mice supplemented with TMC3115 were observed. There was no marked difference in the diversity of cecal bacteria between the tested mice. Elevated serum levels of inflammatory markers such as tumor necrosis factor (TNF)-α and interleukin (IL)-6 were observed in the stressed mice, while TMC3115 only reduced the IL-6 level. These findings suggest that TMC3115 supplementation confers tolerance to psychosocial stress in the host through modulation of the gut microbiota and alleviation of stress-induced inflammatory responses. Furthermore, it may be expected to exert prevention and treatment of disorders related to peripheral IL-6, including depression.

Animals exhibit remarkable navigation abilities as if they have an internal compass. Head direction (HD) cells encoding the animal's heading azimuth are found in the brain of several animal species; the HD cell signals are dependent on the vestibular nuclei, where magnetic responsive cells are present in birds. However, it is difficult to determine whether HD cell signals drive the compass orientation in animals, as they do not necessarily rely on the magnetic compass under all circumstances. Recording of HD cell activities from the medial pallium of shearwater chicks ( Calonectris leucomelas ) just before their first migration, during which they strongly rely on compass orientation, revealed that shearwater HD cells prefer a north orientation. The preference remained stable regardless of geolocations and environmental cues, suggesting the existence of a magnetic compass regulated by internally generated HD signals. Our findings provide insight into the integration of the direction and magnetoreception senses.