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9 results on '"MESH: Mucormycosis"'

1. Posttraumatic mucormycosis: a nationwide study in France and review of the literature

Author

Emmanuel GYAN, Norbert IFRAH, Stéphane Bretagne, Frédéric GRENOUILLET, Marcio Junior, Sophie Cassaing, Jean-Francois Viel, Bruno MARCHOU, Raoul Herbrecht, Daniel Ajzenberg, Catherine Quantin, Cedric Arvieux, Mikael Hivelin, Stéphane Ranque, Boualem Sendid, Centre d'infectiologie Necker-Pasteur [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Centre National de Référence Mycologie et Antifongiques-Mycologie Moléculaire (CNRMA), Institut Pasteur [Paris], Service d'informatique médicale et biostatistiques [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Necker - Enfants Malades [AP-HP], Service de Chirurgie plastique, reconstructrice et esthétique [Pompidou], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpital Européen Georges Pompidou [APHP] (HEGP), Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Université Paris Descartes - Paris 5 (UPD5), Service de parasitologie et de mycologie médicales [Amiens], Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie, Matrice extracellulaire et dynamique cellulaire - UMR 7369 (MEDyC), SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Universitaire de Reims (CHU Reims), Pathogénie des infections systémiques (UMR_S 570), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Université Paris Descartes - Paris 5 (UPD5), Laboratoire de parasitologie et de mycologie médicales [CHU Amiens], CHU Amiens-Picardie, Institut Pasteur [Paris] (IP)-CHU Necker - Enfants Malades [AP-HP], Institut Pasteur [Paris] (IP), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Centre National de Référence Mycologie et Antifongiques-Mycologie Moléculaire ( CNRMA ), Assistance publique - Hôpitaux de Paris (AP-HP)-Hôpital Européen Georges Pompidou [APHP] ( HEGP ) -Université Paris Descartes - Paris 5 ( UPD5 ), Université de Picardie Jules Verne ( UPJV ) -CHU Amiens-Picardie, Matrice extracellulaire et dynamique cellulaire - UMR 7369 ( MEDyC ), Centre National de la Recherche Scientifique ( CNRS ) -Université de Reims Champagne-Ardenne ( URCA ) -SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne ( URCA ) -Université de Picardie Jules Verne ( UPJV ) -Université de Reims Champagne-Ardenne ( URCA ) -Université de Picardie Jules Verne ( UPJV ), Centre Hospitalier Universitaire de Reims ( CHU Reims ), Pathogénie des infections systémiques ( UMR_S 570 ), and Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS )

Subjects
MESH : Aged, Poison control, MESH: Aged, 80 and over, MESH : Child, MESH: Child, MESH : Female, Young adult, MESH: Aged, MESH: Middle Aged, biology, [ SDV.SPEE ] Life Sciences [q-bio]/Santé publique et épidémiologie, General Medicine, MESH : Adult, MESH : Wounds and Injuries, 3. Good health, MESH : Mucorales, MESH: Young Adult, Cohort, Coinfection, MESH: Mucorales, Antifungal, medicine.medical_specialty, medicine.drug_class, MESH : Male, MESH : Young Adult, MESH : Adolescent, Internal medicine, Injury prevention, medicine, MESH : Middle Aged, MESH : Mucormycosis, MESH : France, MESH : Aged, 80 and over, MESH: Mucormycosis, MESH: Adolescent, MESH: Humans, business.industry, MESH : Humans, Mucormycosis, MESH: Adult, Saksenaea vasiformis, medicine.disease, biology.organism_classification, MESH: Male, Surgery, MESH: France, MESH: Wounds and Injuries, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, business, MESH: Female
Abstract

International audience; Data on clinical, mycologic characteristics, and outcome of posttraumatic mucormycosis are scarce and often limited to case reports. From the French nationwide "RetroZygo" study, we compared posttraumatic mucormycosis cases with other forms of mucormycosis. We also reviewed reports of posttraumatic mucormycosis in the English-language literature from 1993 to 2013. We included all proven or probable cases for which underlying condition, route of infection, surgical and antifungal treatments, and outcome were detailed. From our cohort, posttraumatic mucormycosis (n = 16) differed significantly from other forms (n = 85) by rarity of underlying disease (31.2% vs 81%, p

Published
2014
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2. Local control of rhino-orbito-cerebral mucormycosis dramatically impacts survival

Author

O. Lortholary, Dea Garcia-Hermoso, Philippe Herman, Caroline Elie, Romain Kania, P. Vironneau, Fanny Lanternier, Gloria Morizot, Service d'oto-rhino-laryngologie, Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpital Lariboisière, Centre National de Référence des Mycoses invasives et antifongiques - Mycologie moléculaire (CNRMA), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Service des Maladies infectieuses et tropicales [CHU Necker], Université Paris Descartes - Paris 5 (UPD5)-CHU Necker - Enfants Malades [AP-HP]-Institut des Maladies Génétiques Imagine [Paris], CHU Necker - Enfants Malades [AP-HP], Appendix 1. Members of the French Mycosis Study Group and ENT surgeons in this study Besançon (A. Dussaucy, J.F. Viel, F. Grenouillet, G. Blasco, F. Legrand, J. C. Navellou, E. Plouvier, L. Tavernier, B. Kantelip), Bordeaux (A. Kostrzewa, B. Couprie, J. P. Bebear, E. Blanchart, L. De Gabory, M. Dupon, C. Deminière), Dijon (C. Quantin, D. Caillot, C. Duvillard), Nice (G. Daideri, M. Gari-Toussaint, H. Hyvernat, M. Poiree, P. M. Roger), Nimes (C. Lechiche, L Lachaud), Perpignan (M. Saada, C. Billotet, J.M. Lopez), Rennes (A. Fresnel, X. Carsin, J. P. Gangneux, C. Arvieux, T. Lamy de la Chapelle, N. Rioux), Toulouse (L. Molinier, S. Cassaing, M. Alvarez, L. Esposito, B. Marchou, X. Brousset, E. Uro-Coste, E. Serrano). Those in Paris are as follows: Bichat (C. Chochillon, O. Brugière, M. Wolff, V. Joly, H. Adle- Biassette), Lariboisière (T. Meas, R. Kania, P.Herman, E. Sauvaget), Necker (C. Lebihan, M. E. Bougnoux), Pitié Salpétrière (A. Fekkar, A. Datry, L. Bodin, Y. Samson), Saint-Louis (C. Lacroix, P. Ribaud, A. Xhaard, A. Bergeron-Lafaurie, O. Marie, J. Pavie, E. Raffoux)., Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Lariboisière-Fernand-Widal [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), and Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Male, MESH: Combined Modality Therapy, Antifungal Agents, MESH: Skull Base, MESH: Brain Diseases, MESH: Paranasal Sinus Diseases, Rhino orbito cerebral mucormycosis, mucormycosis, necrosis, surgery, 0302 clinical medicine, MESH: Aged, 80 and over, MESH: Bone Diseases, Infectious, Orbital Diseases, 030212 general & internal medicine, Sinusitis, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, Aged, 80 and over, Skull Base, MESH: Aged, Brain Diseases, 0303 health sciences, MESH: Middle Aged, General Medicine, Middle Aged, Bone Diseases, Infectious, Combined Modality Therapy, 3. Good health, Survival Rate, Infectious Diseases, MESH: Young Adult, Female, Adult, Microbiology (medical), medicine.medical_specialty, MESH: Survival Rate, sinusitis, Young Adult, 03 medical and health sciences, Paranasal Sinus Diseases, medicine, Humans, MESH: Debridement, Reference standards, Aged, Retrospective Studies, MESH: Mucormycosis, MESH: Humans, MESH: Orbital Diseases, 030306 microbiology, business.industry, Mucormycosis, MESH: Adult, MESH: Retrospective Studies, Surgical procedures, medicine.disease, MESH: Antifungal Agents, MESH: Male, Surgery, Debridement, Local control, business, MESH: Female
Abstract

International audience; Surgery and antifungals are the reference standard for rhino-orbito-cerebral mucormycosis (ROCM) treatment. The impact of local control on survival of 22 consecutive ROCM adults was studied on day 90: none vs. 75% died, respectively, with or without local control (p

Published
2014
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3. [Laboratory diagnosis of mucormycosis]

Author

Dea Garcia-Hermoso, Centre National de Référence des Mycoses invasives et antifongiques - Mycologie moléculaire (CNRMA), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), and Dea Garcia Heroso déclare participer à des conférences pour l’entreprise Gilead Sciences

Subjects
Mucorales, medicine.medical_specialty, MESH: Diabetes Mellitus, MESH: Organ Transplantation, Disease, MESH: Phenotype, General Biochemistry, Genetics and Molecular Biology, Organ transplantation, 03 medical and health sciences, 0302 clinical medicine, MESH: Immunocompromised Host, Medicine, 030212 general & internal medicine, Intensive care medicine, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, Histological examination, MESH: Mucormycosis, Alternative methods, 0303 health sciences, MESH: Humans, biology, 030306 microbiology, business.industry, Mucormycosis, General Medicine, biology.organism_classification, medicine.disease, 3. Good health, MESH: DNA, Fungal, MESH: Mucorales, business, Solid organ transplantation
Abstract

International audience; Mucormycosis are deep infections caused by ubiquitous filamentous fungi of the order of Mucorales. The disease occurs mostly in immunocompromised, diabetic or solid organ transplant recipients. There are currently no specific diagnostic guidelines for mucormycosis. The histological examination and culture of the clinical sample remain the most useful approaches for diagnosis. Furthermore, alternative methods to the fungal culture are yet to be standardized. Here we review the current microbiological approaches used for the diagnosis and identification of Mucorales.; Les mucormycoses sont des infections invasives provoquées par des champignons filamenteux ubiquitaires appartenant l’ordre des Mucorales. Elles surviennent notamment chez des patients immunodéprimés, diabétiques ou ayant subi une transplantation d’organe. À l’heure actuelle, il n’existe pas de recommandations françaises pour le diagnostic de laboratoire des mucormycoses. Ce diagnostic repose principalement sur l’examen histologique et la mise en culture de l’échantillon clinique. Il n’existe pas de techniques standardisées qui soient une alternative au diagnostic microbiologique. Nous récapitulons ici les méthodes microbiologiques utilisées dans la démarche diagnostique de la maladie ainsi que dans l’identification des Mucorales.

Published
2013
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4. [Laboratory diagnosis of mucormycosis]

Author

Garcia-Hermoso, Dea, Centre National de Référence des Mycoses invasives et antifongiques - Mycologie moléculaire (CNRMA), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Dea Garcia Heroso déclare participer à des conférences pour l’entreprise Gilead Sciences, and Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)

Subjects
MESH: Diabetes Mellitus, MESH: Humans, MESH: Organ Transplantation, Organ Transplantation, MESH: Phenotype, MESH: DNA, Fungal, Immunocompromised Host, Phenotype, Diabetes Mellitus, Mucorales, MESH: Immunocompromised Host, MESH: Mucorales, Humans, Mucormycosis, DNA, Fungal, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, MESH: Mucormycosis
Abstract

International audience; Mucormycosis are deep infections caused by ubiquitous filamentous fungi of the order of Mucorales. The disease occurs mostly in immunocompromised, diabetic or solid organ transplant recipients. There are currently no specific diagnostic guidelines for mucormycosis. The histological examination and culture of the clinical sample remain the most useful approaches for diagnosis. Furthermore, alternative methods to the fungal culture are yet to be standardized. Here we review the current microbiological approaches used for the diagnosis and identification of Mucorales.; Les mucormycoses sont des infections invasives provoquées par des champignons filamenteux ubiquitaires appartenant l’ordre des Mucorales. Elles surviennent notamment chez des patients immunodéprimés, diabétiques ou ayant subi une transplantation d’organe. À l’heure actuelle, il n’existe pas de recommandations françaises pour le diagnostic de laboratoire des mucormycoses. Ce diagnostic repose principalement sur l’examen histologique et la mise en culture de l’échantillon clinique. Il n’existe pas de techniques standardisées qui soient une alternative au diagnostic microbiologique. Nous récapitulons ici les méthodes microbiologiques utilisées dans la démarche diagnostique de la maladie ainsi que dans l’identification des Mucorales.

Published
2013
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5. Experimental murine model of disseminated infection by Saksenaea vasiformis : successful treatment with posaconazole

Author

Françoise Dromer, A. W. Fothergill, Enrique Calvo, Dea Garcia-Hermoso, Eduardo Alvarez, Emilio Mayayo, Josep Guarro, Deanna A. Sutton, Valentina Salas, F. Javier Pastor, Universitat Rovira i Virgili, The University of Texas at San Antonio (UTSA), Centre National de Référence des Mycoses invasives et antifongiques - Mycologie moléculaire (CNRMA), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), and Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Male, Posaconazole, Antifungal Agents, Fungus, Microbial Sensitivity Tests, Microbiology, murine model, 03 medical and health sciences, Mice, 0302 clinical medicine, Amphotericin B, MESH: Amphotericin B, medicine, Animals, Mucormycosis, MESH: Animals, 030212 general & internal medicine, MESH: Mice, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, MESH: Treatment Outcome, MESH: Mucormycosis, 0303 health sciences, MESH: Microbial Sensitivity Tests, biology, 030306 microbiology, Broth microdilution, General Medicine, Saksenaea vasiformis, Triazoles, biology.organism_classification, MESH: Antifungal Agents, Virology, In vitro, posaconazole, MESH: Male, 3. Good health, Disease Models, Animal, Infectious Diseases, Treatment Outcome, S. vasiformis, MESH: Triazoles, Murine model, Mucorales, MESH: Mucorales, Immunocompetence, MESH: Disease Models, Animal, medicine.drug
Abstract

International audience; We have determined the in vitro activity of amphotericin B (AMB) and posaconazole (PSC) against Saksenaea vasiformis using broth microdilution and disk diffusion methods and determined the minimal fungicidal concentration (MFC). PSC was found to have the greatest in vitro activity in all cases and was the most efficacious in prolonging survival and reducing the fungal load in an immunocompetent murine model of disseminated infection caused by four strains of the fungus.

Published
2012
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6. Molecular Phylogeny and Proposal of Two New Species of the Emerging Pathogenic Fungus Saksenaea

Author

Françoise Dromer, Josep Guarro, Joan Cano, Damien Hoinard, Dea Garcia-Hermoso, Michael G. Rinaldi, A. W. Fothergill, Alberto M. Stchigel, Deanna A. Sutton, Eduardo Alvarez, Universitat Rovira i Virgili, Centre National de Référence des Mycoses invasives et antifongiques - Mycologie moléculaire (CNRMA), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), The University of Texas at San Antonio (UTSA), This work was supported by the Spanish Ministerio de Ciencia y Tecnología grant CGL 2009-08698/BOS, with cofunding from Fondo Europeo de Desarrollo Regional (FEDER)., We thank Catalina Nuñez for technical assistance. The NRCMA gratefully acknowledges the technical help of Laure Diancourt and Coralie Tran at the sequencing facility (PF-8, Genotyping of Pathogens and Public Health, Institut Pasteur)., and Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)

Subjects
MESH: Sequence Analysis, DNA, Antifungal Agents, Saksenaea, 0302 clinical medicine, Peptide Elongation Factor 1, Environmental Microbiology, 030212 general & internal medicine, DNA, Fungal, Mycological Typing Techniques, MESH: Phylogeny, Phylogeny, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, 0303 health sciences, Fungal protein, MESH: Microbial Sensitivity Tests, biology, Sporangium, Temperature, MESH: Environmental Microbiology, MESH: Naphthalenes, Pathogenic fungus, Spores, Fungal, MESH: Temperature, Molecular phylogenetics, Mucorales, MESH: Mucorales, MESH: Fungal Proteins, Itraconazole, Microbiology (medical), MESH: Terbinafine, Molecular Sequence Data, Mycology, Microbial Sensitivity Tests, Naphthalenes, Microbiology, Fungal Proteins, 03 medical and health sciences, MESH: Spores, Fungal, MESH: Mycological Typing Techniques, DNA, Ribosomal Spacer, Humans, Mucormycosis, MESH: Peptide Elongation Factor 1, Internal transcribed spacer, Terbinafine, MESH: DNA, Ribosomal Spacer, MESH: Mucormycosis, MESH: Humans, MESH: Molecular Sequence Data, 030306 microbiology, Saksenaea vasiformis, Sequence Analysis, DNA, Triazoles, biology.organism_classification, MESH: Antifungal Agents, MESH: DNA, Fungal, MESH: Triazoles, MESH: Itraconazole
Abstract

Saksenaea is a monotypic genus belonging to the order Mucorales and capable of producing severe human infections. Through a polyphasic study based on analysis of the sequences of the internal transcribed spacer (ITS) region, domains D1 and D2 of the 28S rRNA gene, and the elongation factor 1α (EF-1α) gene, as well as by evaluation of relevant morphological and physiological characteristics of a set of clinical and environmental strains, we have demonstrated that Saksenaea vasiformis is a complex of species. We propose as new species Saksenaea oblongispora , characterized by oblong sporangiospores and unable to grow at 42°C, and Saksenaea erythrospora , characterized by large sporangiophores and sporangia and by ellipsoid sporangiospores, biconcave in the lateral view. Itraconazole, posaconazole, and terbinafine were active against all isolates included in the study, while amphotericin B, voriconazole, and the echinocandins showed low activity.

Published
2010
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7. New evidence of the involvement of Lichtheimia corymbifera in farmer's lung disease

Author

Bénédicte Rognon, Stéphane Bretagne, Sandrine Roussel, Jean-Charles Dalphin, Anne-Pauline Bellanger, Laurence Millon, Charline Candido, Gabriel Reboux, Françoise Botterel, Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Parasitologie-Mycologie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Service de parasitologie [Mondor], Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), and Assistance publique - Hôpitaux de Paris (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 ( UPEC UP12 )

Subjects
MESH: Inflammation, MESH: Interleukin-13, Allergy, MESH : RNA, Messenger, Lichtheimia corymbifera, Gene Expression, MESH : Farmer's Lung, 0302 clinical medicine, Absidia, MESH: Up-Regulation, MESH : Up-Regulation, [ SDV.MP.MYC ] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, 0303 health sciences, Interleukin-13, biology, Farmer's lung, General Medicine, Precipitin, MESH : Epithelial Cells, 3. Good health, Up-Regulation, Infectious Diseases, MESH : Mucorales, MESH: Epithelial Cells, MESH: Mucorales, Mucorales, Hypersensitivity pneumonitis, MESH : Interleukin-8, MESH: Cell Line, Tumor, MESH: Gene Expression, Microbiology, 03 medical and health sciences, Cell Line, Tumor, MESH : Interleukin-13, medicine, Humans, Mucormycosis, MESH : Mucormycosis, RNA, Messenger, MESH: RNA, Messenger, MESH: Mucormycosis, Pneumonitis, MESH: Farmer's Lung, Inflammation, MESH : Inflammation, MESH: Humans, MESH : Cell Line, Tumor, 030306 microbiology, MESH : Humans, fungi, Interleukin-8, Epithelial Cells, biology.organism_classification, medicine.disease, MESH: Interleukin-8, Spore, MESH : Gene Expression, 030228 respiratory system, Immunology, Farmer's Lung
Abstract

International audience; Farmer's lung disease (FLD) is a form of hypersensitivity pneumonitis resulting from recurrent exposure to moldy plant materials. We investigated and compared the initial response of respiratory epithelium after exposure to extracts of Sacharopolyspora rectivirgula, Lichtheimia corymbifera (formerly Absidia corymbifera), Eurotium amstelodami and Wallemia sebi. The two criteria for selection of these species were their high prevalence in the hay handled by FLD patients and the presence of high levels of specific precipitins to these molds in FLD patients' sera. Hydrosoluble extracts were prepared from spores and hyphae grown in culture under optimal conditions for each of the four species. Confluent A549 cells were inoculated with one of the four calibrated soluble extracts. Two mediators, one inflammatory (Interleukin (IL)-8) and one allergic (IL-13), were quantified using real-time PCR and ELISA assay, after four exposure periods (30 min, 2 h, 4 h and 8 h). S. rectivirgula and L. corymbifera extracts were the only ones which induced a marked upregulation of IL-8, as shown by both real-time PCR and ELISA assay 8 h after the initial contact. This study adds to the growing body of evidence that L. corymbifera should be recognized as an etiologic agent of FLD along with S. rectivirgula.

Published
2010
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8. Usefulness of molecular biology performed with formaldehyde-fixed paraffin embedded tissue for the diagnosis of combined pulmonary invasive mucormycosis and aspergillosis in an immunocompromised patient

Author

Véronique Hofman, Michele Baumann, Nicolas Venissac, Dea Garcia-Hermoso, Paul Hofman, Martine Gari-Toussaint, Bernard Padovani, Abdelmajid Dhouibi, Catherine Butori, Gieri Cathomas, Laboratory of Clinical and Experimental Pathology, Louis Pasteur Hospital, Human Biobank, Department of Radiology, Laboratory of Mycology, Archet II Hospital, Centre National de Référence Mycologie et Antifongiques-Mycologie Moléculaire (CNRMA), Institut Pasteur [Paris], Laboratory for Molecular and Infectious Disease Pathology, Cantonal Institute for Pathology, Department of Thoracic Surgery, and Institut Pasteur [Paris] (IP)

Subjects
Male, MESH: Fatal Outcome, Pathology, Tissue Fixation, MESH: Paraffin Embedding, Case Report, MESH: Formaldehyde, Aspergillosis, Polymerase Chain Reaction, MESH: Kidney Transplantation, Aspergillus fumigatus, Fatal Outcome, 0302 clinical medicine, MESH: Rhizopus, MESH: Immunocompromised Host, MESH: Fixatives, Lung, MESH: Aged, Invasive Pulmonary Aspergillosis, 0303 health sciences, Paraffin Embedding, biology, General Medicine, MESH: Predictive Value of Tests, 3. Good health, MESH: Staining and Labeling, medicine.anatomical_structure, 030220 oncology & carcinogenesis, MESH: Immunosuppressive Agents, MESH: Aspergillus fumigatus, MESH: Tomography, X-Ray Computed, Bronchoalveolar Lavage Fluid, Immunosuppressive Agents, Rhizopus, medicine.drug, lcsh:RB1-214, Mucorales, MESH: Pneumonia, MESH: Invasive Pulmonary Aspergillosis, Rhizopus microsporus, medicine.medical_specialty, Histology, Pathology and Forensic Medicine, Fixatives, Immunocompromised Host, Necrosis, 03 medical and health sciences, Predictive Value of Tests, Formaldehyde, medicine, lcsh:Pathology, Humans, Mucormycosis, MESH: Lung, MESH: Mucormycosis, Aged, MESH: Necrosis, Voriconazole, MESH: Humans, Staining and Labeling, MESH: Bronchoalveolar Lavage Fluid, 030306 microbiology, MESH: Polymerase Chain Reaction, Pneumonia, biology.organism_classification, medicine.disease, Kidney Transplantation, MESH: Male, MESH: Tissue Fixation, Tomography, X-Ray Computed, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

Immunocompromised patients who develop invasive filamentous mycotic infections can be efficiently treated if rapid identification of the causative fungus is obtained. We report a case of fatal necrotic pneumonia caused by combined pulmonary invasive mucormycosis and aspergillosis in a 66 year-old renal transplant recipient. Aspergillus was first identified during the course of the disease by cytological examination and culture (A. fumigatus) of bronchoalveolar fluid. Hyphae of Mucorales (Rhizopus microsporus) were subsequently identified by culture of a tissue specimen taken from the left inferior pulmonary lobe, which was surgically resected two days before the patient died. Histological analysis of the lung parenchyma showed the association of two different filamentous mycoses for which the morphological features were evocative of aspergillosis and mucormycosis. However, the definitive identification of the associative infection was made by polymerase chain reaction (PCR) performed on deparaffinized tissue sections using specific primers for aspergillosis and mucormycosis. This case demonstrates that discrepancies between histological, cytological and mycological analyses can occur in cases of combined mycotic infection. In this regard, it shows that PCR on selected paraffin blocks is a very powerful method for making or confirming the association of different filamentous mycoses and that this method should be made available to pathology laboratories.

Published
2010
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9. Molecular and phenotypic evaluation of Lichtheimia corymbifera (formerly Absidia corymbifera) complex isolates associated with human mucormycosis: rehabilitation of L. ramosa

Author

Dea Garcia-Hermoso, Françoise Dromer, Damien Hoinard, Eric Dannaoui, Jean-Charles Gantier, Frédéric Grenouillet, Mycologie moléculaire, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Chrono-environnement (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), and Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)

Subjects
Male, MESH: Sequence Analysis, DNA, Antifungal Agents, Lichtheimia corymbifera, MESH : Aged, chemistry.chemical_compound, Peptide Elongation Factor 1, MESH: Aged, 80 and over, Absidia, RNA, Ribosomal, 28S, Genotype, MESH : Female, MESH: Absidia, DNA, Fungal, Mycological Typing Techniques, Clade, MESH : Mycological Typing Techniques, MESH : DNA, Ribosomal Spacer, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, [ SDV.MP.MYC ] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, Aged, 80 and over, MESH: Aged, MESH: Microbial Sensitivity Tests, 0303 health sciences, MESH: Middle Aged, Melezitose, Middle Aged, MESH : Adult, MESH: RNA, Ribosomal, 28S, MESH : Phenotype, Phenotype, MESH : Peptide Elongation Factor 1, Female, Adult, Microbiology (medical), Adolescent, Sequence analysis, MESH : Male, MESH : Antifungal Agents, Mycology, Microbial Sensitivity Tests, Biology, MESH: Phenotype, Microbiology, 03 medical and health sciences, Species Specificity, MESH: Mycological Typing Techniques, MESH : Adolescent, DNA, Ribosomal Spacer, Humans, Mucormycosis, MESH: Species Specificity, MESH : Species Specificity, MESH : Middle Aged, MESH : Mucormycosis, MESH: Peptide Elongation Factor 1, Internal transcribed spacer, MESH : Aged, 80 and over, MESH: DNA, Ribosomal Spacer, Ribosomal DNA, Aged, MESH: Mucormycosis, 030304 developmental biology, MESH: Adolescent, MESH: Humans, 030306 microbiology, MESH : Humans, MESH: Adult, Sequence Analysis, DNA, MESH: Antifungal Agents, biology.organism_classification, MESH: Male, MESH: DNA, Fungal, MESH : RNA, Ribosomal, 28S, chemistry, MESH : Microbial Sensitivity Tests, MESH : Absidia, MESH : DNA, Fungal, MESH: Female, MESH : Sequence Analysis, DNA
Abstract

Thirty-eight isolates (including 28 isolates from patients) morphologically identified as Lichtheimia corymbifera (formerly Absidia corymbifera ) were studied by sequence analysis (analysis of the internal transcribed spacer [ITS] region of the ribosomal DNA, the D1-D2 region of 28S, and a portion of the elongation factor 1α [EF-1α] gene). Phenotypic characteristics, including morphology, antifungal susceptibility, and carbohydrate assimilation, were also determined. Analysis of the three loci uncovered two well-delimited clades. The maximum sequence similarity values between isolates from both clades were 66, 95, and 93% for the ITS, 28S, and EF-1α loci, respectively, with differences in the lengths of the ITS sequences being detected (763 to 770 bp for isolates of clade 1 versus 841 to 865 bp for isolates of clade 2). Morphologically, the shapes and the sizes of the sporangiospores were significantly different among the isolates from both clades. On the basis of the molecular and morphological data, we considered isolates of clade 2 to belong to a different species named Lichtheimia ramosa because reference strains CBS 269.65 and CBS 270.65 (which initially belonged to Absidia ramosa ) clustered within this clade. As neotype A. corymbifera strain CBS 429.75 belongs to clade 1, the name L. corymbifera was conserved for clade 1 isolates. Of note, the amphotericin B MICs were significantly lower for L. ramosa than for L. corymbifera ( P < 0.005) but were always ≤0.5 μg/ml for both species. Among the isolates tested, the assimilation of melezitose was positive for 67% of the L. ramosa isolates and negative for all L. corymbifera isolates. In conclusion, this study reveals that two Lichtheimia species are commonly associated with mucormycosis in humans.

Published
2009
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