84 results on '"Ning-Ning Li"'
Search Results
2. Molecular mechanisms underlying the BIRC6-mediated regulation of apoptosis and autophagy
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Shuo-Shuo Liu, Tian-Xia Jiang, Fan Bu, Ji-Lan Zhao, Guang-Fei Wang, Guo-Heng Yang, Jie-Yan Kong, Yun-Fan Qie, Pei Wen, Li-Bin Fan, Ning-Ning Li, Ning Gao, and Xiao-Bo Qiu
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Science - Abstract
Abstract Procaspase 9 is the initiator caspase for apoptosis, but how its levels and activities are maintained remains unclear. The gigantic Inhibitor-of-Apoptosis Protein BIRC6/BRUCE/Apollon inhibits both apoptosis and autophagy by promoting ubiquitylation of proapoptotic factors and the key autophagic protein LC3, respectively. Here we show that BIRC6 forms an anti-parallel U-shaped dimer with multiple previously unannotated domains, including a ubiquitin-like domain, and the proapoptotic factor Smac/DIABLO binds BIRC6 in the central cavity. Notably, Smac outcompetes the effector caspase 3 and the pro-apoptotic protease HtrA2, but not procaspase 9, for binding BIRC6 in cells. BIRC6 also binds LC3 through its LC3-interacting region, probably following dimer disruption of this BIRC6 region. Mutation at LC3 ubiquitylation site promotes autophagy and autophagic degradation of BIRC6. Moreover, induction of autophagy promotes autophagic degradation of BIRC6 and caspase 9, but not of other effector caspases. These results are important to understand how the balance between apoptosis and autophagy is regulated under pathophysiological conditions.
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- 2024
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3. Seedling Petri-dish inoculation method: A robust, easy-to-use and reliable assay for studying plant–Ralstonia solanacearum interactions
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Peng CAO, Jia-lan CHEN, Ning-ning LI, Shuang-xi ZHANG, Rong-bo WANG, Ben-jin LI, Pei-qing LIU, Yu-yan AN, and Mei-xiang ZHANG
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bacterial wilt ,Ralstonia solanacearum ,Petri-dish inoculation method ,virulence ,resistance ,defense priming ,Agriculture (General) ,S1-972 - Abstract
Ralstonia solanacearum causes a lethal bacterial wilt disease in many crops, leading to huge losses in crop production every year. Understanding of plant–R. solanacearum interactions will aid to develop efficient strategies to control the disease. As a soilborne pathogen, R. solanacearum naturally infects plants via roots. A huge limitation in studying plant–R. solanacearum interactions is the large variation of R. solanacearum infection assay due to the variable soil conditions and uneven inoculum exposure. Here, we developed a robust and reliable Petri-dish inoculation method which allows consistent and stable infection in young plant seedlings. This method is easy to use, takes about only 10 days from seed germination to the completion of inoculation assay, and requires less inoculum of bacteria as well as growth chamber space. We proved the efficacy of the seedling Petri-dish inoculation method by analyzing plant defense primed by molecular patterns, resistance of defense-related plant mutants, and virulence of R. solanacearum mutants. Furthermore, we demonstrated that the seedling Petri-dish inoculation method can be applied to other host plants such as tobacco and has great potential for high-throughput screening of resistant plant germplasms to bacterial wilt in the future.
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- 2023
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4. Invasive clonal plants possess greater capacity for division of labor than natives in high patch contrast environments
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Jin Zhang, Wen-Hua You, Ning-Ning Li, and Dao-Lin Du
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plant invasion ,clonal plants ,clonal integration ,heterogeneity ,root to shoot ratio ,Plant culture ,SB1-1110 - Abstract
Invasion success of clonal plants is closely related to their unique clonal life history, and clonal division of labor is a crucial clonal trait. However, so far, it is unclear whether invasive alien clonal species generally possess a greater capacity for division of labor than native species and whether this pattern is affected by environmental conditions. To test whether patch contrast affects the differences in the capacity for division of labor between invasive alien and native clonal plants, we selected five pairs of exotic invasive and native clonal plant species that are congeneric and co-occurring in China as experimental materials. We grew the clonal fragment pairs of these invasive and native plants under high, low, or no contrast of reciprocal patchiness of light and nutrient, respectively, with ramet connections either severed (division of labor prevented) or kept intact (division of labor allowed). The results showed that connection significantly decreased the proportion of biomass allocated to roots in distal (younger) ramets, whereas it increased in proximal (older) ramets of all studied plants under high -contrast treatments. This clear pattern strongly indicated the occurrence of division of labor. Furthermore, the connection had a more pronounced effect on the pattern of biomass allocation of invasive alien plants, resulting in a greater increase in biomass for invasive alien plants compared to native plants. These findings suggest that the invasive alien plants possess a greater capacity for division of labor, which may confer a competitive advantage to them over natives, thus facilitating their invasion success in some heterogeneous habitats such as forest edges where light and soil nutrients show a high negative correlation.
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- 2023
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5. Clinical features of Cytomegalovirus retinitis in patients with acquired immunodeficiency syndrome and efficacy of the current therapy
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Qing Zhao, Ning-ning Li, You-xin Chen, and Xin-yu Zhao
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Cytomegalovirus retinitis ,acquired immunodeficiency syndrome ,clinical feature ,efficacy ,systematic review ,meta-analysis ,Microbiology ,QR1-502 - Abstract
BackgroundCytomegalovirus retinitis (CMVR) is the most common and sight-threatening opportunistic retinal infection in patients with acquired immunodeficiency syndrome (AIDS) and several controversies remain to be settled. We aimed to summarize the current evidence and clarify the clinical features and prognosis of CMVR in AIDS patients.MethodsThe databases PubMed, EMBASE, and Ovid from inception to April 2022 were searched to identify the relevant studies. R software version 3.6.3 was used to perform the statistical analyses. Results in proportion with 95% confidence interval (CI) were calculated using the Freeman-Tukey variant of arcsine square transformation.ResultsWe finally included 236 studies comprising 20,214 patients. CMVR in AIDS was male-dominated (88%, 95%CI 86%-89%), with 57% (95%CI 55%-60%) aged
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- 2023
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6. Protein deubiquitylase USP3 stabilizes Aurora A to promote proliferation and metastasis of esophageal squamous cell carcinoma
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Ke Shi, Jin Zhong Zhang, Liang Yang, Ning-Ning Li, Ying Yue, Xiu-Hong Du, Xiu-Zhi Zhang, Yu Cheng Lu, and Dan Guo
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Aurora A ,USP3 ,Ubiquitination ,EMT ,Esophageal squamous cell carcinoma ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Aurora A kinase is a cell cycle regulator that is dysregulated in several different malignancies. Nevertheless, its regulatory mechanisms are still not fully understood. Here, we report that ubiquitin specific peptidase 3 (USP3) promotes proliferation and metastasis of esophageal squamous cell carcinoma (ESCC) cells by mediating deubiquitination of Aurora A. Analysis of human clinical samples indicated that USP3 and Aurora A are highly expressed in ESCC. Cellular experiments confirmed that high expression of USP3 and Aurora A in ESCC cells promoted malignant cell proliferation and invasion. In this mechanism, USP3 leads to suppression of Aurora A ubiquitination, resulting less proteasome degradation. We constructed the deubiquitinated mimetic K143R of Aurora A and found that K143R significantly promoted the proliferation and invasion of ESCC cells and was not regulated by the deubiquitination of USP3. Moreover, Aurora A K143R potentiated the kinase activity of Aurora A in ESCC cells. Thus, our findings demonstrate that the tumorigenic feature of ESCC is in part mediated by USP3-facilitated deubiquitination of Aurora A.
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- 2021
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7. Circulating activated lymphocyte subsets as potential blood biomarkers of cancer progression
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Ying‐Yi Wang, Na Zhou, Hong‐sheng Liu, Xiao‐Lei Gong, Rui Zhu, Xiao‐Yuan Li, Zhao Sun, Xu‐Hong Zong, Ning‐Ning Li, Chang‐Ting Meng, Chun‐Mei Bai, and Tai‐Sheng Li
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cancer development ,cancer progression ,clinicopathologic characteristics ,lymphocyte subsets ,marker ,peripheral blood ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract The objective of this study was to predict the value of lymphocyte subsets in cancer progression. Peripheral blood was obtained from 327 untreated patients with cancer and 158 healthy volunteers. Levels of lymphocyte subsets were determined by flow cytometry. There were decreased levels of natural killer (NK) cells, CD8+ T cells, and naïve CD4+/CD4+ T cells in untreated patients with cancer compared to those in healthy controls. Inversely, there were elevated levels of the following T‐cell percentages in cancer patients compared to those in healthy controls: memory CD4+/CD4+, CD8+ T cells, HLA‐DR/CD8+, CD8+ CD38+/CD8+, and CD4+/CD8+. In addition, there are a decreasing trend in terms of CD4+ T‐cell counts and an increase CD8+ HLA‐DR/CD8+ T‐cell and CD8+ CD38+/CD8+ T‐cell percentages in the advanced stage. An increasing trend with advanced tumor stage and the percentages of CD8+ HLA‐DR/CD8+ T cells and CD8+ CD38+/CD8+ T cells was shown in this study. There are a negative correlation for CD4+ T‐cell counts and positive correlation for percentages of CD8+ HLA‐DR/CD8+ T cell and CD8+ CD38+/CD8+ T cells with the lymph node metastasis. In the presence of distant metastatic spread, we observed higher NK‐cell counts, CD8+ HLA‐DR/CD8+ T‐cell percentages, CD8+ CD38+/CD8+ T‐cell percentages, as well as lower CD4+ T‐cell counts than those in the absence of distant metastases spread. Abnormal levels of NK cell, CD8+ T cells, memory CD4+/CD4+, naïve CD4+/ CD4+, CD8+ HLA‐DR/CD8+, CD8+ CD38+/CD8+, and CD4+/CD8+ can be a potential blood biomarkers of cancer development. CD4+ T‐cell counts and percentages of CD8+ HLA‐DR/ CD8+ and CD8+ CD38+/ CD8+ can predict the cancer progression.
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- 2020
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8. The Effect of Yijinjing on the Cognitive Function of Patients With Chronic Schizophrenia
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Hui Gao, Chao Luo, Si-Jing Tu, Ru-Ping Lu, Lin-Na Jiang, Hui-Jun Qiao, Qu Lin, Ning-Ning Li, and Jian-Hua Chen
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aerobic exercise ,cognitive function ,psychosomatic medicine ,Qigong ,schizophrenia ,traditional Chinese medicine ,Psychiatry ,RC435-571 - Abstract
Background: Patients with chronic schizophrenia present cognitive impairment, which affects their social function and prevents them from reintegrating into society. Yijinjing is a traditional Chinese aerobic exercise that has a putative psychosomatic effect on improving cognitive function.Methods: From January to May 2021, 40 patients with chronic schizophrenia were recruited and randomly divided into a control group and a Yijinjing group. In the 12-week intervention, the patients in the control group received conventional treatment, whereas patients in the Yijinjing group performed Yijinjing exercise (40 min/session, twice a week) in addition to receiving conventional treatment. The Positive and Negative Syndrome Scale (PANSS), the Insight and Treatment Attitude Questionnaire (ITAQ), the Rosenberg Self-esteem Scale (SES), and the Mini Mental State Examination (MMSE) were used to measure clinical symptoms and cognitive function at 0, 6, and 12 weeks.Results: The demographic information was not significantly different between groups. At baseline, the scores of all the scales were not statistically different between groups. After 12 weeks of intervention, compared to those at baseline, the scores of the negative scale (t = 19.00, p < 0.0001), general psychopathology scale (t = 15.98, p < 0.0001), and total score (t = 15.47, p < 0.0001) of the PANSS and SES (t = 5.378, p < 0.0001) had significantly decreased, and the scores of the ITAQ (t = 7.984, p < 0.0001) and MMSE (t = 6.750, p < 0.0001) had significantly increased in Yijinjing group; the score of the MMSE increased in the control group as well (t = 2.491, p = 0.0222). Compared to the respective scores in the control group, the negative scale score (t = 2.953, p = 0.0054) significantly decreased, and the ITAQ (t = 3.043, p = 0.0042) and MMSE (t = 2.2.68, p = 0.0291) scores significantly increased in the Yijinjing group after 12 weeks of intervention.Conclusion: These results provide a preliminary indication that Yijinjing exercise had the potential to improve cognitive function and negative symptoms in patients with chronic schizophrenia. A larger-scale study to determine the trajectory of change in the longer term should be undertaken.
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- 2021
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9. Surgery-related characteristics, efficacy, safety and surgical team satisfaction of three-dimensional heads-up system versus traditional microscopic equipment for various vitreoretinal diseases
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Xin-yu Zhao, Qing Zhao, Ning-ning Li, Li-hui Meng, Wen-fei Zhang, Er-qian Wang, and You-xin Chen
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Cellular and Molecular Neuroscience ,Ophthalmology ,Sensory Systems - Abstract
Purpose To compare the three-dimensional (3D) heads-up surgery with the traditional microscopic (TM) surgery for various vitreoretinal diseases. Methods A medical record review of patients that underwent 3D heads-up or TM vitreoretinal surgeries was performed from May 2020 to October 2021 in this retrospective case–control study. Main outcome measures included surgery-related characteristics, efficacy, safety, and satisfaction feedback from the surgical team. Results A total of 220 (47.6%) and 242 (52.4%) eyes were included in the 3D and TM groups, respectively. The 3D heads-up system significantly benefits delicate surgical steps, like the epiretinal membrane (ERM) peeling for ERM and internal limiting membrane peeling for idiopathic macular holes (P P = 0.049), while no difference by TM surgery (P = 0.45). For the satisfaction feedback, the 3D heads-up system was rated significantly higher in most subscales and the overall score (P P P > 0.05). Conclusion The efficacy and safety of the 3D heads-up surgery were generally comparable to the TM surgery. The 3D heads-up system could significantly benefit delicate surgical steps and achieve better surgical team satisfaction.
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- 2022
10. [Clinical and gastroscopic features of children with cyclic vomiting syndrome: an analysis of 63 cases]
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Lin, Song, Yu-Jia, Li, Jian, Wang, Ning-Ning, Li, Yan-Yan, DU, Lan, He, and Xi-Wei, Xu
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Male ,Sleepiness ,Vomiting ,Bile Reflux ,Anorexia ,Abdominal Pain ,Gastric Mucosa ,Clinical Research ,Gastritis ,Gastroscopy ,Humans ,Female ,Child ,Esophagitis, Peptic ,Retrospective Studies - Abstract
OBJECTIVE: To study the clinical and gastroscopic features of children with cyclic vomiting syndrome. METHODS: A retrospective analysis was performed on the medical data of 63 children with cyclic vomiting syndrome who were hospitalized and followed up in Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University from August 2019 to March 2022. RESULTS: Among the 63 children, there were 30 boys and 33 girls, with a mean age of 6.11 years, a mean course of disease of 2.57 years, and a mean vomiting period of 4.04 days. The most common accompanying symptom was listlessness or somnolence (55/63, 87%), followed by anorexia (45/63, 71%), abdominal pain or abdominal discomfort (40/63, 63%), constipation (19/63, 30%), salivation (12/63, 19%), nausea (11/63, 17%), headache (11/63, 17%), fever (6/63, 10%), and rash (1/63, 2%). All 63 children underwent gastroscopy, among whom 3 had no marked abnormalities, 22 (35%) had chronic superficial gastritis or chronic non-atrophic gastritis alone, and 38 (60%) had other abnormal changes aside from chronic gastritis (16 children with reflux esophagitis, 12 with bile reflux gastritis, 13 with duodenitis, 10 with erosive gastritis, and 5 with gastric or duodenal ulcer). Among the 63 children, 42 underwent pathological examinations of gastric mucosa, among whom 5 had no marked abnormalities, 34 had mild chronic gastritis, 2 had moderate chronic gastritis, and 1 had severe chronic gastritis. Among the 63 children, 15 received 24-hour dynamic esophageal pH monitoring during the interictal period, among whom 9 children were found to have pathological acid reflux. CONCLUSIONS: In addition to recurrent vomiting, most children with cyclic vomiting syndrome also have the symptoms such as somnolence or listlessness, anorexia, and abdominal pain. The main manifestation on gastroscopy is chronic gastritis, and most children may also have reflux esophagitis, bile reflux gastritis, and erosive gastritis. Mild chronic gastritis is the main pathological change of gastric mucosa.
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- 2022
11. Microbiome‐driven anticancer therapy: A step forward from natural products
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Yunxuan Guan, Di Wu, Hui Wang, and Ning‐Ning Liu
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anticancer ,cancer ,chemotherapy ,immunotherapy ,microbiota ,Microbiology ,QR1-502 - Abstract
Abstract Human microbiomes, considered as a new emerging and enabling cancer hallmark, are increasingly recognized as critical effectors in cancer development and progression. Manipulation of microbiome revitalizing anticancer therapy from natural products shows promise toward improving cancer outcomes. Herein, we summarize our current understanding of the human microbiome‐driven molecular mechanisms impacting cancer progression and anticancer therapy. We highlight the potential translational and clinical implications of natural products for cancer prevention and treatment by developing targeted therapeutic strategies as adjuvants for chemotherapy and immunotherapy against tumorigenesis. The challenges and opportunities for future investigations using modulation of the microbiome for cancer treatment are further discussed in this review.
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- 2024
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12. Multi-omics analysis of human tendon adhesion reveals that ACKR1-regulated macrophage migration is involved in regeneration
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Xinshu Zhang, Yao Xiao, Bo Hu, Yanhao Li, Shaoyang Zhang, Jian Tian, Shuo Wang, Zaijin Tao, Xinqi Zeng, Ning-Ning Liu, Baojie Li, and Shen Liu
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Biology (General) ,QH301-705.5 ,Physiology ,QP1-981 - Abstract
Abstract Tendon adhesion is a common complication after tendon injury with the development of accumulated fibrotic tissues without effective anti-fibrotic therapies, resulting in severe disability. Macrophages are widely recognized as a fibrotic trigger during peritendinous adhesion formation. However, different clusters of macrophages have various functions and receive multiple regulation, which are both still unknown. In our current study, multi-omics analysis including single-cell RNA sequencing and proteomics was performed on both human and mouse tendon adhesion tissue at different stages after tendon injury. The transcriptomes of over 74 000 human single cells were profiled. As results, we found that SPP1+ macrophages, RGCC+ endothelial cells, ACKR1+ endothelial cells and ADAM12+ fibroblasts participated in tendon adhesion formation. Interestingly, despite specific fibrotic clusters in tendon adhesion, FOLR2+ macrophages were identified as an antifibrotic cluster by in vitro experiments using human cells. Furthermore, ACKR1 was verified to regulate FOLR2+ macrophages migration at the injured peritendinous site by transplantation of bone marrow from Lysm-Cre;R26R tdTomato mice to lethally irradiated Ackr1 −/− mice (Ackr1 −/− chimeras; deficient in ACKR1) and control mice (WT chimeras). Compared with WT chimeras, the decline of FOLR2+ macrophages was also observed, indicating that ACKR1 was specifically involved in FOLR2+ macrophages migration. Taken together, our study not only characterized the fibrosis microenvironment landscape of tendon adhesion by multi-omics analysis, but also uncovered a novel antifibrotic cluster of macrophages and their origin. These results provide potential therapeutic targets against human tendon adhesion.
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- 2024
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13. KRAS, NRAS, BRAF signatures, and MMR status in colorectal cancer patients in North China
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Shen-Yi Lian, Lu-Xin Tan, Xin-Zhi Liu, Lu-Jing Yang, Ning-Ning Li, Qing Feng, Ping Wang, Yue Wang, Dong-Bo Qiao, Li-Xin Zhou, Ting-Ting Sun, Lin Wang, Ai-Wen Wu, and Zhong-Wu Li
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General Medicine - Published
- 2023
14. Study on elastic mechanical properties of novel 2D negative Poisson’s ratio structure: Re-entrant hexagon nested with star-shaped structure
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Yu-Tong Jin, Yan-Hui Qie, Ning-Ning Li, and Nai-Wen Li
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Ceramics and Composites ,Civil and Structural Engineering - Published
- 2022
15. Global fungal-host interactome mapping identifies host targets of candidalysin
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Tian-Yi Zhang, Yao-Qi Chen, Jing-Cong Tan, Jin-An Zhou, Wan-Ning Chen, Tong Jiang, Jin-Yin Zha, Xiang-Kang Zeng, Bo-Wen Li, Lu-Qi Wei, Yun Zou, Lu-Yao Zhang, Yue-Mei Hong, Xiu-Li Wang, Run-Ze Zhu, Wan-Xing Xu, Jing Xi, Qin-Qin Wang, Lei Pan, Jian Zhang, Yang Luan, Rui-Xin Zhu, Hui Wang, Changbin Chen, and Ning-Ning Liu
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Science - Abstract
Abstract Candidalysin, a cytolytic peptide toxin secreted by the human fungal pathogen Candida albicans, is critical for fungal pathogenesis. Yet, its intracellular targets have not been extensively mapped. Here, we performed a high-throughput enhanced yeast two-hybrid (HT-eY2H) screen to map the interactome of all eight Ece1 peptides with their direct human protein targets and identified a list of potential interacting proteins, some of which were shared between the peptides. CCNH, a regulatory subunit of the CDK-activating kinase (CAK) complex involved in DNA damage repair, was identified as one of the host targets of candidalysin. Mechanistic studies revealed that candidalysin triggers a significantly increased double-strand DNA breaks (DSBs), as evidenced by the formation of γ-H2AX foci and colocalization of CCNH and γ-H2AX. Importantly, candidalysin binds directly to CCNH to activate CAK to inhibit DNA damage repair pathway. Loss of CCNH alleviates DSBs formation under candidalysin treatment. Depletion of candidalysin-encoding gene fails to induce DSBs and stimulates CCNH upregulation in a murine model of oropharyngeal candidiasis. Collectively, our study reveals that a secreted fungal toxin acts to hijack the canonical DNA damage repair pathway by targeting CCNH and to promote fungal infection.
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- 2024
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16. Dysregulation of iron homeostasis and methamphetamine reward behaviors in Clk1-deficient mice
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Peng-ju Yan, Zhaoxiang Ren, Zhi-feng Shi, Liu-shuai Zhu, Ning-ning Li, Chaojun Han, Chun-lei Wan, John L. Waddington, and Xuechu Zhen
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medicine.medical_specialty ,Iron ,Hippocampus ,Striatum ,Article ,Methamphetamine ,chemistry.chemical_compound ,Mice ,Reward ,Internal medicine ,medicine ,Animals ,Homeostasis ,Pharmacology (medical) ,Dopamine transporter ,Pharmacology ,Gene knockdown ,biology ,General Medicine ,Meth ,Psychological dependence ,Conditioned place preference ,Corpus Striatum ,Rats ,Endocrinology ,nervous system ,chemistry ,biology.protein ,medicine.drug - Abstract
Chronic administration of methamphetamine (METH) leads to physical and psychological dependence. It is generally accepted that METH exerts rewarding effects via competitive inhibition of the dopamine transporter (DAT), but the molecular mechanism of METH addiction remains largely unknown. Accumulating evidence shows that mitochondrial function is important in regulation of drug addiction. In this study, we investigated the role of Clk1, an essential mitochondrial hydroxylase for ubiquinone (UQ), in METH reward effects. We showed that Clk1(+/−) mutation significantly suppressed METH-induced conditioned place preference (CPP), accompanied by increased expression of DAT in plasma membrane of striatum and hippocampus due to Clk1 deficiency-induced inhibition of DAT degradation without influencing de novo synthesis of DAT. Notably, significantly decreased iron content in striatum and hippocampus was evident in both Clk1(+/−) mutant mice and PC12 cells with Clk1 knockdown. The decreased iron content was attributed to increased expression of iron exporter ferroportin 1 (FPN1) that was associated with elevated expression of hypoxia-inducible factor-1α (HIF-1α) in response to Clk1 deficiency both in vivo and in vitro. Furthermore, we showed that iron played a critical role in mediating Clk1 deficiency-induced alteration in DAT expression, presumably via upstream HIF-1α. Taken together, these data demonstrated that HIF-1α-mediated changes in iron homostasis are involved in the Clk1 deficiency-altered METH reward behaviors.
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- 2021
17. DFT study of the fouling deposition process in the steam generator by simulating the adsorption of Fe2+ on Fe3O4 (0 0 1)
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San-Chuan Pan, Tetsuo Shoji, Dongbai Sun, Tong Zhang, Jian Xu, Ning-ning Li, Lu Ren, and Hongying Yu
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education.field_of_study ,Materials science ,010304 chemical physics ,Organic Chemistry ,Population ,Thermodynamics ,Ionic bonding ,010402 general chemistry ,01 natural sciences ,Catalysis ,0104 chemical sciences ,Computer Science Applications ,Inorganic Chemistry ,Bond length ,Adsorption ,Molecular geometry ,Computational Theory and Mathematics ,Chemisorption ,0103 physical sciences ,CASTEP ,Density functional theory ,Physical and Theoretical Chemistry ,education - Abstract
In order to reveal the fouling problem on the outer surface of the steam generator (SG) tube in the secondary circuit condition of pressurized water reactor (PWR) nuclear power plant, based on the density functional theory (DFT) method, the Cambridge sequential total energy program package (CASTEP) is used to simulate seven kinds of highly symmetric adsorption structure models of termination with tetrahedral Fe (A termination) and termination with octahedral Fe (B termination) on Fe3O4 (0 0 1) surface. The adsorption energies and stable adsorption conformations are calculated. The results show that the most stable adsorption structures of the Fe2+/Fe3O4 (0 0 1) configurations are Fe2+ above Fe-O bond of B layer termination (Fe3O4(001) A-b). During the adsorption, the Fe-Fe, Fe-O bond length, and Fe-Fe-O bond angle of (0 0 1) surface change, and the atomic positions parallel and perpendicular to (0 0 1) surface change correspondingly. The change happened to the surface layer is the most drastic one. The calculation of charge population, the density of states (DOS), and electron local function of Fe2+/Fe3O4 (0 0 1) optimal adsorption configuration show that there is electron transfer between Fe2+ and Fe3O4 (0 0 1), and the adsorption type is chemisorption. Among them, Fe (Fe2+)-Fe (Fe3O4) forms a metal bond, and Fe (Fe2+)-O (Fe3O4) forms the ionic bond. The results illustrate the interaction between free Fe2+ and Fe3O4 is the reason of the nucleation and agglomeration of Fe3O4 scale and it provides the foundation for the further research on Fe3O4 scale deposition.
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- 2021
18. 'Nutrient–fungi–host' tripartite interaction in cancer progression
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Di Wu, Yun‐Xuan Guan, Chen‐Hao Li, Quan Zheng, Zuo‐Jing Yin, Hui Wang, and Ning‐Ning Liu
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anticancer treatment ,cancer ,fungi ,mycobiome ,nutrients ,Computer applications to medicine. Medical informatics ,R858-859.7 - Abstract
Abstract The human microbiome exhibits a profound connection with the cancer development, progression, and therapeutic response, with particular emphasis on its components of the mycobiome, which are still in the early stages of research. In this review, we comprehensively summarize cancer‐related symbiotic and pathogenic fungal genera. The intricate mechanisms through which fungi impact cancer as an integral member of both gut and tissue‐resident microbiomes are further discussed. In addition, we shed light on the pivotal physiological roles of various nutrients, including cholesterol, carbohydrates, proteins and minerals, in facilitating the growth, reproduction, and invasive pathogenesis of the fungi. While our exploration of the interplay between nutrients and cancer, mediated by the mycobiome, is ongoing, the current findings have yet to yield conclusive results. Thus, delving into the relationship between nutrients and fungal pathogenesis in cancer development and progression would provide valuable insights into anticancer therapy and foster precision nutrition and individualized treatments that target fungi from bench to bedside.
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- 2024
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19. Vancomycin-assisted green synthesis of reduced graphene oxide for antimicrobial applications
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Yi Jian Li, Li Qun Xu, Xuefeng Hu, Ning Ning Li, Yunbing Wang, Jieyu Zhang, Chang Ming Li, and Yan Biao Liao
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Male ,Staphylococcus aureus ,Surface Properties ,medicine.drug_class ,Reducing agent ,Oxide ,Microbial Sensitivity Tests ,02 engineering and technology ,Glycopeptide antibiotic ,Bacterial growth ,010402 general chemistry ,medicine.disease_cause ,01 natural sciences ,law.invention ,Rats, Sprague-Dawley ,Biomaterials ,chemistry.chemical_compound ,Colloid and Surface Chemistry ,Vancomycin ,law ,medicine ,Animals ,Particle Size ,Graphene ,Oxides ,Staphylococcal Infections ,021001 nanoscience & nanotechnology ,Antimicrobial ,Anti-Bacterial Agents ,Rats ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,chemistry ,Graphite ,0210 nano-technology ,Nuclear chemistry ,medicine.drug - Abstract
Chemical reduction of graphene oxide (GO) is a simple and inexpensive method for the large-scale production of graphene-based materials. A suitable reducing agent, especially a green reductant, is in high demand for the production of reduced GO (RGO). Vancomycin, a glycopeptide antibiotic used to treat a variety of Gram-positive bacterial infections, was used to chemically reduce GO at a weak alkaline pH. As far as we know, this is the first report of reduction of GO by a glycopeptide antibiotic. The resulting vancomycin-decorated RGO (RGO-Van) was characterized by UV-visible adsorption and Raman spectroscopies, X-ray diffraction pattern and atomic force microscopy (AFM). The antibacterial effect of the RGO-Van suspension was investigated by the bacterial growth curves. The RGO-Van sheet can be fabricated into paper-like film through vacuum filtration. The antibacterial property of the as-obtained RGO-Van film was assessed by the inhibition zone test, and the bacterial adhesion assay. The antibacterial efficacy of the RGO-Van film was also verified by treatment of wound infection caused by Staphylococcus aureus (S. aureus) in a rat infection model.
- Published
- 2018
20. Computational study of Fe3O4 adsorption behaviour on the secondary side of the heat exchange tube in the steam generator
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Hongying Yu, Tetsuo Shoji, Wen-Yang Jia, Dongbai Sun, Dongyang Zhang, Ning-ning Li, Lu Ren, Jian Xu, and Tong Zhang
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Materials science ,General Computer Science ,Diffusion ,Boiler (power generation) ,General Physics and Astronomy ,Thermodynamics ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Radial distribution function ,01 natural sciences ,0104 chemical sciences ,Mean squared displacement ,Computational Mathematics ,Adsorption ,Mechanics of Materials ,Agglomerate ,Deposition (phase transition) ,General Materials Science ,0210 nano-technology ,Scaling - Abstract
Based on the scaling problem on the outer surface of the steam generator tube in the secondary circuit condition of pressurized water reactor, the molecular dynamic was used to calculate the adsorption characteristics of Fe3O4. The trajectory, mean squared displacement, radial distribution function and adsorption energy of Fe3O4 agglomeration and deposition process were further analyzed through variation of temperature and concentration. When the temperature was between 510 K and 530 K, the diffusion coefficient of Fe2+ and OH− could reach the minimum value of 2.255 × 10-5 cm2/s and 2.638 × 10-5 cm2/s, respectively. During variation of Fe2+ concentration, the size of agglomerate particles increased continuously, and the diffusion coefficient of H2O can reach the minimum value of 3.78 × 10-5 cm2/s. Fe2+ with positive charges can effectively agglomerate with H2O and OH−, forming the tetrahedral and octahedral structures of Fe3O4 crystal. At this time, a small number of OH− were adsorbed at the interface of the matrix, forming the double electrical layer and affecting the deposition of agglomerated Fe3O4. Apart from this, high temperature is beneficial to inhibit scaling deposition in part. Likewise, low concentration of the dissolved Fe2+ and OH− can also lead to weaker deposition of agglomerated Fe3O4 on the outer scaling layer of the secondary side in the steam generator.
- Published
- 2021
21. Host–microbiota interaction during cancer progression from bulk to single‐cell level
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Yong‐Jing Ma, Run‐Ze Zhu, and Ning‐Ning Liu
- Subjects
cancer ,host–microbiota interaction ,polymorphic microbiome ,single‐cell analysis ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Abstract Background Cancer, a global threat to human health, refers to a large crowd of lethal diseases that can start in almost all tissues or organs of our body when abnormal cells grow uncontrollably and beyond their usual boundaries, followed by invading adjacent normal tissues and spreading to distant organs. Recently, substantial evidence has demonstrated polymorphic microbiome as an emerging cancer characteristic present in a variety of tumour types. By releasing metabolites or other signalling molecules, microbiota can affect immune cell differentiation and activity, including both innate and adaptive immunological responses, as well as the growth and multiplication of cancer cells. It was now employed as a biomarker to predict the patients’ survival rate and the degree of cancer progression. Main To investigate the underlying mechanism and the technology development of how polymorphic microbiome influence the tumour microenvironment and subsequently cancer progression, we have carried out systematic literature review about polymorphic microbiome and tumour development from bulk to single‐cell level. Conclusions Here, we provide an overview of the current advancements of host–microbiota interactions during cancer progression from bulk to single‐cell level and discuss the challenges and opportunities in future, looking forward to ascertain the specific activity of different somatic cell types with or without the existence of various multi‐kingdom microbiota and their by‐products and supply a holistic and elaborate investigation of host‐microbiota interaction in the development of cancer.
- Published
- 2024
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22. DFT studies on the interaction of Fe2+/Fe3O4(1 1 1) and OH-/Fe3O4(1 1 1) during the adsorption process in the steam generators of nuclear power plants
- Author
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San-Chuan Pan, Hongying Yu, Ning-ning Li, Dongbai Sun, Tong Zhang, Lu Ren, Dongyang Zhang, Tetsuo Shoji, and Jian Xu
- Subjects
education.field_of_study ,Materials science ,Population ,Nucleation ,02 engineering and technology ,Crystal structure ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Electron transfer ,Colloid and Surface Chemistry ,Adsorption ,Chemical bond ,Density of states ,Physical chemistry ,Density functional theory ,0210 nano-technology ,education - Abstract
Based on the fouling problem on the outer surface of the steam generator tube in the secondary circuit condition of pressurized water reactors, the density functional theory was used to calculate the adsorption characteristics of Fe2+, OH− on Fe3O4 surface. Eight kinds of highly symmetric adsorption configurations of Fe2+ and OH− on Fe3O4 (1 1 1) surface under two kinds of Fetet1- and Feoct2-terminated surfaces were established. Meantime, the interaction mechanisms and electronic structures of Fe2+/Fe3O4(1 1 1), OH−/Fe3O4(1 1 1) before and after the adsorption process were studied. The results demonstrated that the most stable adsorption site of Fe2+/Fe3O4(1 1 1) is the Fe3O4(111) tet-b configuration. After Fe2+ adsorbed on Fe3O4(111) tet-b sites, the lengths of Fe-Fe and Fe-O bonds and the Fe-Fe-O angle changed. The parallel and vertical directions of top atoms in Fe3O4(1 1 1) surface changed and the shift of topmost atoms was the largest, indicating that the Fe3O4 crystal lattice parameters can be affected by the Fe2+ adsorption. The charge population, density of states, and electron local function results indicated that there were 0.5e to 0.81e electron transfer between Fe2+ and Fe3O4 (1 1 1). The electron transfer of OH-/Fe3O4(111) oct-Oh is from 0.18e to 0.63e. Both of the Fe2+ and OH- can form the chemical bond on the Fe3O4(1 1 1) surface. The results illustrate the nucleation and agglomeration of Fe3O4 scale and provide the foundation for the further research on Fe3O4 scale deposition.
- Published
- 2021
23. Thyroid metastasis from non-small cell lung cancer
- Author
-
Jun, Cao, Yan-Er, Yu, Ning-Ning, Li, Yuan-Xi, Wu, Jia-Ni, Shi, and Mei-Yu, Fang
- Subjects
endocrine system ,endocrine system diseases ,Original Article - Abstract
Non-thyroid malignancies to the thyroid gland resulting from distant metastases are extremely rare, and such cases are rarely seen in clinical settings. The question of how a tumor metastasizes to the thyroid remains unanswered. Here we report a case of lung adenocarcinoma metastasizing to the thyroid gland. The article covers the pathological features, treatments, examination reports, and the postoperative follow-up reviews of the patient. In this article, we discuss the diagnostic method, the spread route, the prognosis, the mechanism and above all, the treatment. In addition, we searched the PubMed and ISI Web of Science databases for articles published in English using the key words "lung", "thyroid", and "metastasis", and we reviewed nearly all the reports about thyroid malignancies being metastasized from lung cancer. This rare case emphasizes the importance of the multifaceted comprehensiveness of the cephalometry diagnosis, pathological diagnosis, and immunohistochemical analysis to ensure that such rare cases are not missed. We declare that all cases of thyroid malignancies metastasized from the lungs shall be reported at large for further clinical research.
- Published
- 2019
24. New therapeutic strategies for IPF: Based on the 'phagocytosis-secretion-immunization' network regulation mechanism of pulmonary macrophages
- Author
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Zinan Qin, Hua-Qiang Zhai, Tian Zhang, Xin Liu, Guo-Xiu Liu, Ning-Ning Li, Jiajia Chen, Min Gu, and Si-Yu Li
- Subjects
0301 basic medicine ,Chemokine ,Network regulation ,Phagocytosis ,Pulmonary Fibrosis ,RM1-950 ,Pathogenesis ,03 medical and health sciences ,0302 clinical medicine ,Immunity ,Traditional Chinese medicines ,Pulmonary fibrosis ,Macrophages, Alveolar ,medicine ,Humans ,Secretion ,Pharmacology ,biology ,Mechanism (biology) ,business.industry ,Macrophages ,Interstitial lung disease ,General Medicine ,Fibroblasts ,medicine.disease ,030104 developmental biology ,030220 oncology & carcinogenesis ,Immunology ,biology.protein ,Cytokines ,Immunization ,Therapeutics. Pharmacology ,business - Abstract
Pulmonary fibrosis is a chronic and progressive interstitial lung disease of known and unknown etiology. Over the past decades, macrophages have been recognized to play a significant role in IPF pathogenesis. According to their anatomical loci, macrophages can be divided to alveolar macrophages (AMs) subtypes and interstitial macrophages subtypes (IMs) with different responsibility in the damage defense response. Depending on diverse chemokines and cytokines in local microenvironments, macrophages can be induced and polarized to either classically activated (M1) or alternatively activated (M2) phenotypes in different stages of immunity. Therefore, we hypothesize that there is a “phagocytosis-secretion-immunization” network regulation of pulmonary macrophages related to a number of chemokines and cytokines. In this paper, we summarize and discuss the role of chemokines and cytokines involved in the “phagocytosis-secretion-immunization” network regulation mechanism of pulmonary macrophages, pointing toward novel therapeutic approaches based on the network target regulation in the field. Therapeutic strategies focused on modifying the chemokines, cytokines and the network are promising for the pharmacotherapy of IPF. Some Traditional Chinese medicines may have more superiorities in delaying the progression of pulmonary fibrosis for their multi-target activities of this network regulation.
- Published
- 2019
25. Fault Detection of Liquid-Propellant Rocket Engines Based on LSSVM
- Author
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Ning-ning Li, Wei Xue, Liang Xu, Xiang Guo, and Song-bo Zhao
- Subjects
Support vector machine ,Artificial neural network ,Control theory ,Liquid-propellant rocket ,business.industry ,Computer science ,Least squares support vector machine ,Particle swarm optimization ,Rocket engine ,business ,Fault (power engineering) ,Fault detection and isolation - Abstract
In terms of fault diagnosis of liquid-propellant rocket engine, the fault diagnosis accuracy of the traditional method is low the characteristics of engine fault data are small sample and nonlinear variation. In order to improve the accuracy of sensor fault diagnosis and overcome the scarcity of related samples, the particle swarm optimization (PSO) with strong global searching ability is used to optimize the LS-SVM parameters, and the LSSVM optimal parameter values are obtained by iteration to improve the model fitting accuracy and generalization ability. At the same time, the traditional support vector machine and BP neural network model are used for the detection. The simulation results show that the least squares support vector machine (SVM) detection method based on particle swarm optimization has the advantages of high precision and high speed. It has certain effect and positive significance for improving the safety of liquid-propellant rocket engine test and engine failure loss.
- Published
- 2018
26. Fatal pulmonary Kaposi sarcoma in an HIV seronegative AIDS patient
- Author
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Tai-sheng Li, Huan-ling Wang, Ning-ning Li, Ding-rong Zhong, Yi Xiao, Hong Zhang, Wei Zhang, and Yan Liu
- Subjects
0301 basic medicine ,Pulmonary and Respiratory Medicine ,medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,AIDS-Related Opportunistic Infections ,030106 microbiology ,Mucocutaneous zone ,virus diseases ,medicine.disease ,Virology ,Dermatology ,03 medical and health sciences ,0302 clinical medicine ,Fine-needle aspiration ,Acquired immunodeficiency syndrome (AIDS) ,Immunology and Allergy ,Medicine ,030212 general & internal medicine ,Sarcoma ,business ,Genetics (clinical) ,Immunodeficiency ,HIV Seronegativity ,Lung Kaposi Sarcoma - Abstract
Introduction Although HIV antibody tests have been widely accepted in clinical diagnosis of HIV infection, they may not be sufficient to diagnose all subjects with HIV infection. Except negative result of antibody test in the well-known" acute window phase", in rare cases, patients do not develop HIV antibodies despite demonstrable infection. Primary pulmonary Kaposi sarcoma (KS) without mucocutaneous involvement accounts for only 0-15% of all AIDS-related KS. KS is rare among Chinese subjects, especially in persons of Han descent. Methods A case of seronegative AIDS with primary pulmonary Kaposi sarcoma (KS) was reported. It's a 46-year-old Chinese man presented with sore throat, hemoptysis, fever, dyspnea and multiple lung nodules. The lung lesions grew over a 5-month period so as the symptoms worsened. The possibility of AIDS was discounted by his physicians because of the repeatedly negative HIV antibodies tests despite the ELISA tests or Western blot tests. Histopathologic diagnosis of fine needle lung biopsy in local hospital was undetermined. After admission, HIV infection was eventually confirmed by plasma HIV RNA testing. Histopathologic diagnosis of Lung Kaposi sarcoma was made through repeated fine needle aspiration biopsy as well as the review of former one. Multiple antibiotics and chemotherapy were administrated with no clinical effect due to advanced stage and the patient passed away soon after diagnosis. Results This is the first case of seronegative HIV-1 infection with presentation of primary pulmonary KS. Conclusion This case underscores the importance of plasma RNA test in conjunction with HIV antibody test for some rare patients with HIV infection who present with severe immunodeficiency and opportunistic infections or malignancy.
- Published
- 2016
27. Development and treatment of colorectal cancer: Insights from multi‐kingdom microbiota
- Author
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Yue‐Mei Hong, Dingka Song, Ning‐Ning Liu, and Hui Wang
- Subjects
cancer therapeutics ,colorectal cancer ,diet ,multi‐kingdom microbiota ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 ,Geriatrics ,RC952-954.6 - Abstract
Abstract Colorectal cancer (CRC) is a multistage and highly heterogeneous malignant disease that mostly occurred in aged people accompanied by microbiota alteration. Emerging evidence has uncovered the role of bacterial microbiota in the initiation and development of CRC. However, the effects of nonbacterial members inhabiting the human body, such as fungi, archaea, and viruses, have been largely ignored. The multi‐kingdom microbiota can be altered by dietary exposures and probiotic supplements. Furthermore, the efficacy of antitumor therapeutic strategies, such as radiotherapy, chemotherapy, and immunotherapy, are also closely associated with the alteration of multi‐kingdom microbiota. In this review, we describe CRC‐associated multi‐kingdom microbiota dysbiosis and the role of daily diet on CRC progression through microbiota alteration. We then discuss the impact of microbiota in different CRC therapies and highlight the advances as well as challenges in understanding how multi‐kingdom microbiome impacts the outcome of CRC.
- Published
- 2023
- Full Text
- View/download PDF
28. Quaternized poly(2-(dimethylamino)ethyl methacrylate)-grafted agarose copolymers for multipurpose antibacterial applications
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Jiu Cun Chen, Ning Ning Li, Guodong Fu, En-Tang Kang, and Li Qun Xu
- Subjects
Biofouling ,chemistry.chemical_compound ,Tertiary amine ,Atom-transfer radical-polymerization ,Chemistry ,General Chemical Engineering ,Polymer chemistry ,Self-healing hydrogels ,Copolymer ,Agarose ,General Chemistry ,Methacrylate ,Antibacterial activity - Abstract
Polymeric quaternary ammonium salts (QAS) have been widely studied for their antimicrobial activities. However, most of them were evaluated and used in solution form, which may limit their other applications, in medical and wound care and surface biofouling prevention. The development of antibacterial agents for use both in solution and in surface biofouling prevention are highly interesting and desirable. In this work, quaternized poly(2-(dimethylamino)ethyl methacrylate)-grafted agarose (Agr-g-QPDMAEMA) copolymers were synthesized via a combination of atom transfer radical polymerization (ATRP) and quaternization of tertiary amine moieties. The resulting Agr-g-QPDMAEMA copolymers can be dissolved in aqueous media at low concentrations to evaluate their antibacterial activity in the solution form. They can also be gelated as hydrogels on substrate surfaces to inhibit bacterial adhesion, biofilm formation and bacterial colonization. The Agr-g-QPDMAEMA copolymer hydrogels can be further fabricated into antibacterial patches to inhibit the bacteria growth on the contaminated surfaces.
- Published
- 2015
29. Profiles of metabolic gene expression in the white adipose tissue, liver and hypothalamus in leptin knockout (Lep
- Author
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Lei-Jian, Guan, Kai-Xuan, Xu, Shu-Yang, Xu, Ning-Ning, Li, Xin-Ru, Wang, Yan-Kai, Xia, and Di, Wu
- Subjects
Lep ΔI14/ΔI14 ,Original Article ,microarray analysis ,hypothalamus ,liver ,white adipose - Abstract
Leptin deficiency is principally linked to metabolic disorders. Leptin knockout (LepΔI14/ΔI14) Sprague Dawley rats created by CRISPR/Cas9 is a new model to study metabolic disorders. We used a whole rat genome oligonucleotide microarray to obtain tissue-specific gene expression profiles of the white adipose tissue, liver and hypothalamus inLepΔI14/ΔI14 and wild-type (WT) rats. We found 1,651 differentially expressed (enriched) genes in white adipose tissue, 916 in the liver, and 306 in the hypothalamus in theLepΔI14/ΔI14 rats compared to WT. Gene ontology category and KEGG pathway analysis of the relationships among differentially expressed genes showed that these genes were represented in a variety of functional categories, including fatty acid metabolism, molecular transducers and cellular processes. The reliability of the data obtained from microarray was verified by quantitative real-time PCR on 14 representative genes. These data will contribute to a greater understanding of different metabolic disorders, such as obesity and diabetes.
- Published
- 2017
30. Primary Cardiac Synovial Sarcoma
- Author
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Ji-Gang Wang and Ning-Ning Li
- Subjects
Adult ,Male ,Pulmonary and Respiratory Medicine ,Oncology ,medicine.medical_specialty ,Pathology ,Adolescent ,Risk Assessment ,Disease-Free Survival ,Cohort Studies ,Heart Neoplasms ,Sarcoma, Synovial ,Young Adult ,Rare Diseases ,Internal medicine ,medicine ,Overall survival ,Humans ,Neoplasm Invasiveness ,Cardiac Surgical Procedures ,Survival analysis ,Aged ,Neoplasm Staging ,business.industry ,Biopsy, Needle ,Rare entity ,Middle Aged ,Prognosis ,Key features ,Immunohistochemistry ,Magnetic Resonance Imaging ,Survival Analysis ,Cardiac Synovial Sarcoma ,Female ,Surgery ,Neoplasm Recurrence, Local ,Cardiology and Cardiovascular Medicine ,business - Abstract
Primary cardiac synovial sarcoma is an extremely rare entity. The clinical and pathologic characteristics are still poorly understood, and prognostic factors influencing overall survival are still unknown. In the present study, all characteristics of reported patients, including sex, age, clinical presentations, laboratory tests, electrocardiogram, imaging findings, pathology, location, therapy, and follow-up were carefully reviewed and survival analysis was performed. The present study has summarized some key features and may provide an effective consultation for the diagnosis and treatment of the tumor.
- Published
- 2013
31. Primary Discussion on LNG Terminal Fire Prevention System Design
- Author
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Kai Liu, Yu-bao Liu, and Ning-ning Li
- Subjects
Engineering ,Architectural engineering ,LNG terminal ,endocrine system ,Unit system ,business.industry ,media_common.quotation_subject ,Fire prevention ,General Medicine ,Fire prevention system ,Fire risk ,Unit (housing) ,Execution system ,Detection ,Risk analysis (engineering) ,Terminal (electronics) ,Systems design ,business ,Function (engineering) ,Alarm ,Engineering(all) ,media_common - Abstract
At present, our nation attaches more and more importance to development of LNG industry, and LNG terminal is an important part of LNG industry chain. Aiming at current problem of being no basis for LNG terminal fire prevention system design, this article puts forward the primary solution on fire prevention design for such sites by combining fire risk of LNG terminal, requirements of relevant regulations both at home and abroad and practical experience. In this article, LNG terminal fire prevention system consists of detection unit, execution system and extinguishing and alarm unit system. This article introduces the composition and function of each part.
- Published
- 2013
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- View/download PDF
32. Editorial: New insights into the role of mycobiome in diseases
- Author
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Wenjuan Wu, Jun Ding, Xiaoqi Zheng, Hui Wang, and Ning-Ning Liu
- Subjects
mycobiome ,fungi ,interaction ,multi-omic ,diseases ,Microbiology ,QR1-502 - Published
- 2023
- Full Text
- View/download PDF
33. Editorial: Microbiome in IBD: From Composition to Therapy, Volume II
- Author
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Ning-Ning Liu, Peijian He, Zhanju Liu, Ruixin Zhu, Yinglei Miao, Chenggong Yu, and Lixin Zhu
- Subjects
inflammatory bowel disease ,microbiome ,ulcerative colitis ,Crohn’s disease ,interaction ,Therapeutics. Pharmacology ,RM1-950 - Published
- 2023
- Full Text
- View/download PDF
34. Biomimetic anchors applied to the host-guest antifouling functionalization of titanium substrates
- Author
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Li Qun Xu, Xiao Yan Cai, Jiu Cun Chen, En-Tang Kang, and Ning Ning Li
- Subjects
Biofouling ,Surface Properties ,Inorganic chemistry ,Oxide ,chemistry.chemical_element ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Bacterial Adhesion ,Biomaterials ,Contact angle ,chemistry.chemical_compound ,Colloid and Surface Chemistry ,Adsorption ,Biomimetic Materials ,Escherichia coli ,Particle Size ,Titanium ,Molecular Structure ,Adhesion ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Titanium oxide ,chemistry ,Chemical engineering ,Surface modification ,0210 nano-technology ,Protein adsorption - Abstract
A biomimetic strategy was developed for the construction of antifouling titanium oxide (Ti(oxide)) surfaces based on host-guest interactions. Two catecholic derivatives, dopamine 4-(phenylazo)benzamide (AZODopa) and dopamine 1-adamantanecarboxamide (AdaDopa) were synthesized and immobilized onto the Ti(oxide) surfaces. The guest molecules-anchored Ti(oxide) surfaces were further functionalized with zwitterionic heptakis[6-deoxy-6-(N-3-sulfopropyl-N,N-dimethylammonium ethyl sulfanyl)]-β-cyclodextrin (SBCD) and hydrophilic β-CD polymer (CDP). The surface elemental compositions and hydrophobic/hydrophilic properties of the Ti(oxide) surfaces before and after modification were characterized by X-ray photoelectron spectroscopy (XPS) and static water contact angle measurements, respectively. The antifouling properties of the modified Ti(oxide) surfaces were evaluated by the protein adsorption and bacterial adhesion assays. The zwitterionic SBCD- and hydrophilic CDP-functionalized Ti(oxide) surfaces can reduce the adsorption of bovine plasma fibrinogen and adhesion of Escherichia coli, as compared to the pristine and guest molecules-anchored Ti(oxide) surfaces.
- Published
- 2016
35. Neutron reflectometry of anionic surfactants on sapphire: A strong maximum in the adsorption near the critical micelle concentration
- Author
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Adrian R. Rennie, Robert Thomas, and Ning Ning Li
- Subjects
Sodium ,Inorganic chemistry ,Thermodynamics of micellization ,Analytical chemistry ,chemistry.chemical_element ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Biomaterials ,Colloid and Surface Chemistry ,Adsorption ,chemistry ,Pulmonary surfactant ,Critical micelle concentration ,Caesium ,Neutron reflectometry ,0210 nano-technology ,Saturation (chemistry) - Abstract
The adsorption of the anionic surfactants, lithium, sodium and cesium dodecylsulfates, and sodium decylsulfonate, on the positively charged C-plane (0001) of sapphire (alumina) has been measured using neutron reflection. For each of the four surfactants there is a strong maximum in the adsorption at about the critical micelle concentration. The maximum becomes more marked from lithium to cesium. The measurements were reproduced over a range of different physical conditions and could not be accounted for in terms of impurities. The maximum is explained quantitatively by using the combination of a mass action model to calculate the mean activity of the surfactant, and a cooperative model of the adsorption (Frumkin), in which saturation of the layer is not attained until well above the critical micelle concentration.
- Published
- 2016
36. PEGylated Fluorescent Nanoparticles from One-Pot Atom Transfer Radical Polymerization and 'Click Chemistry'
- Author
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Li Qun Xu, Jiu Cun Chen, En-Tang Kang, Ning Ning Li, and Bin Zhang
- Subjects
Materials science ,Polymers and Plastics ,Atom-transfer radical-polymerization ,fluorescent nanoparticles ,technology, industry, and agriculture ,Chain transfer ,ATRP ,click chemistry ,one-pot synthesis ,PEGylated ,cellular imaging ,General Chemistry ,Fluorene ,lcsh:QD241-441 ,chemistry.chemical_compound ,lcsh:Organic chemistry ,Suzuki reaction ,Polymerization ,chemistry ,Polymer chemistry ,Click chemistry ,Copolymer ,Ethylene glycol ,health care economics and organizations - Abstract
The preparation of PEGylated fluorescent nanoparticles (NPs) based on atom transfer radical polymerization (ATRP) and “click chemistry” in one-pot synthesis is presented. First, poly(p-chloromethyl styrene-alt-N-propargylmaleimide) (P(CMS-alt-NPM)) copolymer was prepared via reversible addition-fragmentation chain transfer (RAFT) polymerization. Subsequently, the azido-containing fluorene-based polymer, poly[(9,9-dihexylfluorene)-alt-(9,9-bis-(6-azidohexyl)fluorene)] (PFC6N3), was synthesized via Suzuki coupling polymerization, followed by azidation. Finally, the PEGylated fluorescent NPs were prepared via simultaneous intermolecular “click” cross-linking between P(CMS-alt-NPM) and PFC6N3 and the ATRP of poly(ethylene glycol) methyl ether methacrylate (PEGMMA) using P(CMS-alt-NPM) as the macroinitiator. The low cytotoxicity of the PEGylated fluorescent NPs was revealed by incubation with KB cells, a cell line derived from carcinoma of the nasopharynx, in an in vitro experiment. The biocompatible PEGylated fluorescent NPs were further used as a labeling agent for KB cells.
- Published
- 2015
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37. MicroRNA expression profiles in familial hypertrophic cardiomyopathy with myosin-binding protein C3 (MYBPC3) gene mutations
- Author
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Li-rong Lin, Xue-qun Hu, Li-hong Lu, Jia-zhen Dai, Ning-ning Lin, Re-hua Wang, Zhang-xin Xie, and Xue-mei Chen
- Subjects
Familial hypertrophic cardiomyopathy ,MYBPC3 ,Gene mutation ,MicroRNA-sequencing ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Abstract Familial hypertrophic cardiomyopathy (FHCM) is an autosomal dominant inherited disease caused by mutations in genes encoding cardiac sarcomere proteins. MicroRNAs (miRNAs) play an important role in the pathogenesis of FHCM. In the present study, we aimed to determine the miRNA profile in FHCM patients with myosin-binding protein C3 (MYBPC3) gene mutations. We recruited three FHCM patients and age- and sex-matched controls. The three probands all had hypertrophic obstructive cardiomyopathy with severe myocardial hypertrophy, and two of the three had a history of sudden cardiac death, representing a “malignant” phenotype. We then compared the miRNA expression profiles of three FHCM patients carrying MYBPC3 gene mutations with those of the normal control group using miRNA sequencing technology. Differentially expressed miRNAs were verified using real-time polymerase chain reaction (qPCR). Target genes and signaling pathways of the identified differentially expressed miRNAs were predicted using bioinformatics analysis. A total of 33 significantly differentially expressed miRNAs were detected in the peripheral blood of the three probands, of which 28 were upregulated, including miR-208b-3p, and 5 were downregulated. Real-time PCR confirmed the upregulated expression of miR-208b-3p in FHCM patients (P
- Published
- 2022
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- View/download PDF
38. Efficacy of intravitreal conbercept combined with panretinal photocoagulation for severe nonproliferative diabetic retinopathy without macular edema
- Author
-
Ning Zhao, Jian Guan, Na Cai, and Ning-Ning Liu
- Subjects
conbercopt ,panretinal photocoagulation ,severe nonproliferative diabetic retinopathy ,Ophthalmology ,RE1-994 - Abstract
AIM: To assess efficacy of intravitreal conbercept (IVC) injection in combination with panretinal photocoagulation (PRP) vs PRP alone in patients with severe nonproliferative diabetic retinopathy (SNPDR) without macular edema (ME). METHODS: Forty-eight patients with SNPDR without ME (56 eyes) were divided into the PRP group and IVC+PRP group (the pulse group) in this retrospective clinical study. Conbercept was intravitreally administered to patients in the pulse group 1wk before treatment with PRP and followed up for 1, 3, and 6mo. The best-corrected visual acuity (BCVA, logMAR), center foveal thickness (CFT), visual acuity (VA) improvement, and adverse reactions were compared between groups. RESULTS: In the PRP group, the BCVA reduced at 1 and 3mo before improving at 6mo. In the pulse group, baseline BCVA decreased continuously at 1mo, increased at 3 and 6mo. BCVA in the pulse group was better than that in the PRP group at 1, 3, and 6mo. There was an increase in CFT in the PRP group during follow-up compared with baseline. In the pulse group, CFT was increased at 1mo relative to baseline, steadily decreased to the baseline level at 3 and 6mo. There was a more significant reduction in CFT in the pulse group during follow-up compared with the PRP group. The effective rates of VA in the PRP and the pulse groups were 81.48% and 100%, respectively. CONCLUSION: As PRP pretreatment, a single dose of IVC administration has beneficial effects for preventing PRP-induced foveal thickening and increasing VA in patients with SNPDR without ME.
- Published
- 2022
- Full Text
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39. Soil erodibility affected by vegetation restoration on steep gully slopes on the Loess Plateau of China
- Author
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Guanghui Zhang, Han-yue Yang, Baojun Zhang, Ning-ning Li, and Hao Wang
- Subjects
Hydrology ,010504 meteorology & atmospheric sciences ,ved/biology ,Soil organic matter ,ved/biology.organism_classification_rank.species ,Soil Science ,04 agricultural and veterinary sciences ,Loess plateau ,Environmental Science (miscellaneous) ,01 natural sciences ,Shrub ,Hydraulic conductivity ,040103 agronomy & agriculture ,Cohesion (geology) ,Root mass ,0401 agriculture, forestry, and fisheries ,Environmental science ,Soil conservation ,0105 earth and related environmental sciences ,Earth-Surface Processes - Abstract
Vegetation restoration influences near soil-surface characteristics and thus likely affects soil erodibility. This study was performed to quantify the effects of vegetation restoration on soil erodibility on steep gully slopes, and to identify the potential influencing factors on the Loess Plateau. Three shrub and four grass types distributed on different gully slopes were selected, and six erodibility indicators and an integrated erodibility index (IEI) were applied to indirectly evaluate the effects of vegetation restoration on soil erodibility. The former included the soil erodibility K factor, aggregate stability (the mean weight diameter, MWD, and the mean number of drop impacts, MND), saturated hydraulic conductivity (Ks), cohesion (Coh), and penetration resistance (PR), and the latter was calculated using these indicators and a weighted integration method. The results showed that vegetation restoration on steep gully slopes was effective in reducing soil erodibility on the Loess Plateau, and grasses seemed more effective than shrubs. Compared with the control, the K of vegetation-restored gully slopes decreased by 4.1–24.0%, and MWD, MND, Ks, Coh, and PR increased by 64.0–284.3, 51.4–269.5, 100.5–417.4, 10.1–172.2, and 63.3–278.9% respectively. Consequently, the IEI of the vegetation-restored gully slopes declined by 33.1–81.9%, and the mean reduction percentage of the four grasses was 1.5 times that of the three shrubs. The variation in soil erodibility was closely related to the changes in the soil organic matter content and root mass density with vegetation restoration. The results will help in understanding the soil conservation mechanisms of vegetation restoration on steep gully slopes.
- Published
- 2018
40. Beneficial insights into postbiotics against colorectal cancer
- Author
-
Dingka Song, Xiuli Wang, Yongjing Ma, Ning-Ning Liu, and Hui Wang
- Subjects
postbiotics ,CRC ,gut microbiota ,nutritional intervention ,immune regulation ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Colorectal cancer (CRC) is one of the most prevalent and life-threatening cancer types with limited therapeutic options worldwide. Gut microbiota has been recognized as the pivotal determinant in maintaining gastrointestinal (GI) tract homeostasis, while dysbiosis of gut microbiota contributes to CRC development. Recently, the beneficial role of postbiotics, a new concept in describing microorganism derived substances, in CRC has been uncovered by various studies. However, a comprehensive characterization of the molecular identity, mechanism of action, or routes of administration of postbiotics, particularly their role in CRC, is still lacking. In this review, we outline the current state of research toward the beneficial effects of gut microbiota derived postbiotics against CRC, which will represent the key elements of future precision-medicine approaches in the development of novel therapeutic strategies targeting gut microbiota to improve treatment outcomes in CRC.
- Published
- 2023
- Full Text
- View/download PDF
41. Fatal pulmonary Kaposi sarcoma in an HIV seronegative AIDS patient
- Author
-
Hong, Zhang, Huan-Ling, Wang, Ding-Rong, Zhong, Yan, Liu, Ning-Ning, Li, Wei, Zhang, Yi, Xiao, and Tai-Sheng, Li
- Subjects
Male ,Acquired Immunodeficiency Syndrome ,Fatal Outcome ,Lung Neoplasms ,AIDS-Related Opportunistic Infections ,HIV Seronegativity ,HIV ,Humans ,HIV Infections ,Middle Aged ,Tomography, X-Ray Computed ,Sarcoma, Kaposi - Abstract
Although HIV antibody tests have been widely accepted in clinical diagnosis of HIV infection, they may not be sufficient to diagnose all subjects with HIV infection. Except negative result of antibody test in the well-known" acute window phase", in rare cases, patients do not develop HIV antibodies despite demonstrable infection. Primary pulmonary Kaposi sarcoma (KS) without mucocutaneous involvement accounts for only 0-15% of all AIDS-related KS. KS is rare among Chinese subjects, especially in persons of Han descent.A case of seronegative AIDS with primary pulmonary Kaposi sarcoma (KS) was reported. It's a 46-year-old Chinese man presented with sore throat, hemoptysis, fever, dyspnea and multiple lung nodules. The lung lesions grew over a 5-month period so as the symptoms worsened. The possibility of AIDS was discounted by his physicians because of the repeatedly negative HIV antibodies tests despite the ELISA tests or Western blot tests. Histopathologic diagnosis of fine needle lung biopsy in local hospital was undetermined. After admission, HIV infection was eventually confirmed by plasma HIV RNA testing. Histopathologic diagnosis of Lung Kaposi sarcoma was made through repeated fine needle aspiration biopsy as well as the review of former one. Multiple antibiotics and chemotherapy were administrated with no clinical effect due to advanced stage and the patient passed away soon after diagnosis.This is the first case of seronegative HIV-1 infection with presentation of primary pulmonary KS.This case underscores the importance of plasma RNA test in conjunction with HIV antibody test for some rare patients with HIV infection who present with severe immunodeficiency and opportunistic infections or malignancy.
- Published
- 2015
42. Repurposing the FDA‐approved anticancer agent ponatinib as a fluconazole potentiator by suppression of multidrug efflux and Pma1 expression in a broad spectrum of yeast species
- Author
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Lin Liu, Tong Jiang, Jia Zhou, Yikun Mei, Jinyang Li, Jingcong Tan, Luqi Wei, Jingquan Li, Yibing Peng, Changbin Chen, Ning‐Ning Liu, and Hui Wang
- Subjects
Biotechnology ,TP248.13-248.65 - Abstract
Summary Fungal infections have emerged as a major global threat to human health because of the increasing incidence and mortality rates every year. The emergence of drug resistance and limited arsenal of antifungal agents further aggravates the current situation resulting in a growing challenge in medical mycology. Here, we identified that ponatinib, an FDA‐approved antitumour drug, significantly enhanced the activity of the azole fluconazole, the most widely used antifungal drug. Further detailed investigation of ponatinib revealed that its combination with fluconazole displayed broad‐spectrum synergistic interactions against a variety of human fungal pathogens such as Candida albicans, Saccharomyces cerevisiae and Cryptococcus neoformans. Mechanistic insights into the mode of action unravelled that ponatinib reduced the efflux of fluconazole via Pdr5 and suppressed the expression of the proton pump, Pma1. Taken together, our study identifies ponatinib as a novel antifungal that enhances drug activity of fluconazole against diverse fungal pathogens.
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- 2022
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43. Conjugation of Lectin to Poly(ε-caprolactone)-block-glycopolymer Micelles for In Vitro Intravesical Drug Delivery
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Xuefeng Hu, Jiu Cun Chen, Xiao Yan Cai, Li Qun Xu, and Ning Ning Li
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Materials science ,Polymers and Plastics ,micelles ,Glycopolymer ,chemical and pharmacologic phenomena ,macromolecular substances ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Micelle ,Article ,lcsh:QD241-441 ,chemistry.chemical_compound ,glycopolymer ,lcsh:Organic chemistry ,lectin ,mucoadhesion ,reactive pentafluorophenyl ester ,bladder cancer ,polycyclic compounds ,Copolymer ,Organic chemistry ,biology ,technology, industry, and agriculture ,Chain transfer ,General Chemistry ,musculoskeletal system ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Polymerization ,chemistry ,Concanavalin A ,Drug delivery ,Biophysics ,biology.protein ,0210 nano-technology ,Caprolactone - Abstract
Amphiphilic poly(ε-caprolactone)-block-poly[2-(α-d-mannopyranosyloxy) ethyl acrylamide] (PCL-b-PManEA) block copolymers were synthesized via a combination of ring-opening polymerization (ROP), reversible addition-fragmentation chain transfer (RAFT) polymerization and reactive ester-amine reaction. The PCL-b-PManEA block copolymers can self-assemble into micelles and encapsulate anticancer drug doxorubicin (DOX). To enhance mucoadhesive property of the resulting DOX-loaded PCL-b-PManEA micelles, Concanavalin A (ConA) lectin was further conjugated with the micelles. Turbidimetric assay using mucin shows that the DOX-loaded PCL-b-PManEA@ConA micelles are mucoadhesive. DOX release from the DOX-loaded PCL-b-PManEA@ConA micelles in artificial urine at 37 °C exhibits an initial burst release, followed by a sustained and slow release over three days. Confocal laser scanning microscope (CLSM) images indicate that the DOX-loaded PCL-b-PManEA@ConA micelles can be effectively internalized by UMUC3 human urothelial carcinoma cells. The DOX-loaded PCL-b-PManEA@ConA micelles exhibit significant cytotoxicity to these cells.
- Published
- 2016
44. ANLN promotes carcinogenesis in oral cancer by regulating the PI3K/mTOR signaling pathway
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Bing Wang, Xiao-li Zhang, Chen-xi Li, Ning-ning Liu, Min Hu, and Zhong-cheng Gong
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ANLN ,Oral cancer ,mTOR ,PI3K signaling pathway ,Specialties of internal medicine ,RC581-951 - Abstract
Abstract Background Oral cancer is a malignant disease that threatenshuman life and greatly reducespatientquality of life. ANLN was reported to promote the progression of cancer. This study aims to investigate the role of ANLNin oral cancer and the underlying molecular mechanism. Methods ANLN expression was downregulated by RNAi technology. The effect of ANLN on cell behaviors, including proliferation, cell cycle progression, invasion, and apoptosis, was detected. Western blotting analysis was used to explore the mechanism by whichANLN functions in oral cancer. Results Data from TCGA database showed that ANLN was expressed at significantly higher levels in tumor tissues thanin normal control tissues. Patients with higher ANLN expression exhibitedshorter survivaltimes. ANLN was alsoabundantly expressedin the cancer cell lines CAL27 and HN30. When ANLN was knocked down in CAL27 and HN30 cells, cell proliferation and colony formation weredecreased. The cell invasion ability was also inhibited. However, the cell apoptosis rate was increased. In addition, the levels of critical members of the PI3K signaling pathway, includingPI3K, mTOR, Akt, and PDK-1, were significantlyreducedafter ANLN was knocked down in CAL27 cells. Conclusions ANLN contributes to oral cancerprogressionand affects activation ofthe PI3K/mTOR signaling pathway. This study providesa new potential targetfor drug development and treatment in oral cancer.
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- 2021
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45. Identification of microbial markers across populations in early detection of colorectal cancer
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Yuanqi Wu, Na Jiao, Ruixin Zhu, Yida Zhang, Dingfeng Wu, An-Jun Wang, Sa Fang, Liwen Tao, Yichen Li, Sijing Cheng, Xiaosheng He, Ping Lan, Chuan Tian, Ning-Ning Liu, and Lixin Zhu
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Science - Abstract
Abstract Associations between gut microbiota and colorectal cancer (CRC) have been widely investigated. However, the replicable markers for early-stage adenoma diagnosis across multiple populations remain elusive. Here, we perform an integrated analysis on 1056 public fecal samples, to identify adenoma-associated microbial markers for early detection of CRC. After adjusting for potential confounders, Random Forest classifiers are constructed with 11 markers to discriminate adenoma from control (area under the ROC curve (AUC) = 0.80), and 26 markers to discriminate adenoma from CRC (AUC = 0.89), respectively. Moreover, we validate the classifiers in two independent cohorts achieving AUCs of 0.78 and 0.84, respectively. Functional analysis reveals that the altered microbiome is characterized with increased ADP-l-glycero-beta-d-manno-heptose biosynthesis in adenoma and elevated menaquinone-10 biosynthesis in CRC. These findings are validated in a newly-collected cohort of 43 samples using quantitative real-time PCR. This work proves the validity of adenoma-specific markers across multi-populations, which would contribute to the early diagnosis and treatment of CRC.
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- 2021
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46. Stress- and metabolic responses of Candida albicans require Tor1 kinase N-terminal HEAT repeats.
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Wanjun Qi, Maikel Acosta-Zaldivar, Peter R Flanagan, Ning-Ning Liu, Niketa Jani, José F Fierro, María T Andrés, Gary P Moran, and Julia R Köhler
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Immunologic diseases. Allergy ,RC581-607 ,Biology (General) ,QH301-705.5 - Abstract
Whether to commit limited cellular resources toward growth and proliferation, or toward survival and stress responses, is an essential determination made by Target of Rapamycin Complex 1 (TORC1) for a eukaryotic cell in response to favorable or adverse conditions. Loss of TORC1 function is lethal. The TORC1 inhibitor rapamycin that targets the highly conserved Tor kinase domain kills fungal pathogens like Candida albicans, but is also severely toxic to human cells. The least conserved region of fungal and human Tor kinases are the N-terminal HEAT domains. We examined the role of the 8 most N-terminal HEAT repeats of C. albicans Tor1. We compared nutritional- and stress responses of cells that express a message for N-terminally truncated Tor1 from repressible tetO, with cells expressing wild type TOR1 from tetO or from the native promoter. Some but not all stress responses were significantly impaired by loss of Tor1 N-terminal HEAT repeats, including those to oxidative-, cell wall-, and heat stress; in contrast, plasma membrane stress and antifungal agents that disrupt plasma membrane function were tolerated by cells lacking this Tor1 region. Translation was inappropriately upregulated during oxidative stress in cells lacking N-terminal Tor1 HEAT repeats despite simultaneously elevated Gcn2 activity, while activation of the oxidative stress response MAP kinase Hog1 was weak. Conversely, these cells were unable to take advantage of favorable nutritional conditions by accelerating their growth. Consuming oxygen more slowly than cells containing wild type TOR1 alleles during growth in glucose, cells lacking N-terminal Tor1 HEAT repeats additionally were incapable of utilizing non-fermentable carbon sources. They were also hypersensitive to inhibitors of specific complexes within the respiratory electron transport chain, suggesting that inefficient ATP generation and a resulting dearth of nucleotide sugar building blocks for cell wall polysaccharides causes cell wall integrity defects in these mutants. Genome-wide expression analysis of cells lacking N-terminal HEAT repeats showed dysregulation of carbon metabolism, cell wall biosynthetic enzymes, translational machinery biosynthesis, oxidative stress responses, and hyphal- as well as white-opaque cell type-associated genes. Targeting fungal-specific Tor1 N-terminal HEAT repeats with small molecules might selectively abrogate fungal viability, especially when during infection multiple stresses are imposed by the host immune system.
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- 2022
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47. Antifungal Nanomaterials: Current Progress and Future Directions
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Jia-Le Xu, Yu-Xuan Luo, Sheng-Hao Yuan, Li-Wen Li, and Ning-Ning Liu
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antifungal ,nanomaterial ,nanoparticle ,Computer applications to medicine. Medical informatics ,R858-859.7 - Abstract
Fungal infection poses a severe threat to human health worldwide resulting in a serious problem in clinic. Due to the limited arsenal of existing antifungal drugs, the nanomaterials were thus regarded as the candidate for developing new antifungal drugs. On the one hand, the antifungal nanomaterials are divided into inorganic nanomaterials, organic nanomaterials, and hybrid nanomaterials, among which inorganic nanoparticles include metal and semiconducting categories. On the other hand, they can also be divided into inorganic particles, organic structures, and mixed nanostructures. Currently various directions for the research and development of antifungal nanomaterials are undergoing. To improve the antifungal effect, the chemical modification of nanomaterials and combination with the available drugs are two strategies widely used. In addition, optimizing the synthetic process of nanomaterials is also a major method to broaden their antifungal application. This review focuses on the current research progress and cutting-edge technologies of antifungal nanomaterials in the field of pharmacodynamics, synthesis and combination of drugs. The nanomaterial will provide a promising and broadly effective antifungal strategy and represent a potentially repositionable candidate for the treatment of fungal infections.
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- 2021
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48. Candidalysin: From Mechanism of Action to Biomarker Development and Therapeutic Response
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Yao-Qi Chen, Qian Li, Tian-Yi Zhang, and Ning-Ning Liu
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candidalysin ,candida albicans ,cytolytic peptide toxin ,fungal infection ,ece1 ,Computer applications to medicine. Medical informatics ,R858-859.7 - Abstract
The incidence of systemic fungal infection is increasing, and millions of people around the world suffer from fungal infections. Candida albicans is one of the most frequently isolated fungal pathogens in clinical settings. As a polymorphic organism, the transition between yeast and hyphae is critical for C. albicans virulence and pathogenesis. However, the mechanism of hyphae-associated virulence remains unclear. Candidalysin is the first human fungal cytolytic peptide toxin originating from the hyphae-specific gene, ECE1. This review will summarize the most recent progress underlying candidalysin-mediated epithelial damage and host defense pathways, which might shed new light on the development of a novel antifungal strategy and early diagnostic biomarker.
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- 2021
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49. Microbiome dysbiosis in lung cancer: from composition to therapy
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Ning-Ning Liu, Qiang Ma, Yang Ge, Cheng-Xiang Yi, Lu-Qi Wei, Jing-Cong Tan, Qiao Chu, Jing-Quan Li, Peng Zhang, and Hui Wang
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract The correlations between microbiota dysbiosis and cancer have gained extensive attention and been widely explored. As a leading cancer diagnosis worldwide, lung cancer poses a great threat to human health. The healthy human lungs are consistently exposed to external environment and harbor a specific pattern of microbiota, sharing many key pathological and physiological characteristics with the intestinal tract. Although previous findings uncovered the critical roles of microbiota in tumorigenesis and response to anticancer therapy, most of them were focused on the intestinal microbiota rather than lung microbiota. Notably, the considerable functions of microbiota in maintaining lung homeostasis should not be neglected as the microbiome dysbiosis may promote tumor development and progression through production of cytokines and toxins and multiple other pathways. Despite the fact that increasing studies have revealed the effect of microbiome on the induction of lung cancer and different disease status, the underlying mechanisms and potential therapeutic strategies remained unclear. Herein, we summarized the recent progresses about microbiome in lung cancer and further discussed the role of microbial communities in promoting lung cancer progression and the current status of therapeutic approaches targeting microbiome to alleviate and even cure lung cancer.
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- 2020
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50. Fungal commensalism modulated by a dual-action phosphate transceptor
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Yuanyuan Wang, Jia Zhou, Yun Zou, Xiaoqing Chen, Lin Liu, Wanjun Qi, Xinhua Huang, Changbin Chen, and Ning-Ning Liu
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Pho84 ,Candida albicans ,gastrointestinal commensalism ,dual-action activity ,Biology (General) ,QH301-705.5 - Abstract
Summary: Successful host colonization by fungi in fluctuating niches requires response and adaptation to multiple environmental stresses. However, our understanding about how fungal species thrive in the gastrointestinal (GI) ecosystem by combing multifaceted nutritional stress with respect to homeostatic host-commensal interactions is still in its infancy. Here, we discover that depletion of the phosphate transceptor Pho84 across multiple fungal species encountered a substantial cost in gastrointestinal colonization. Mechanistically, Pho84 enhances the gastrointestinal commensalism via a dual-action activity, coordinating both phosphate uptake and TOR activation by induction of the transcriptional regulator Try4 and downstream commensalism-related transcription. As such, Pho84 promotes Candida albicans commensalism, but this does not translate into enhanced pathogenicity. Thus, our study uncovers a specific nutrient-dependent dual-action regulatory pathway for Pho84 on fungal commensalism.
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- 2022
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