49 results on '"Pucci D"'
Search Results
2. Spatially Clustered Movement Patterns and Segregation of Subadult Chinook Salmon within the Salish Sea
- Author
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Arostegui, M. C., primary, Smith, J. M., additional, Kagley, A. N., additional, Spilsbury-Pucci, D., additional, Fresh, K. L, additional, and Quinn, T. P., additional
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- 2017
- Full Text
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3. Nature Cell Biology
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Youssef KK, Lapouge G, Bouvrxe9e K, Rorive S, Brohxe9e S, Appelstein O, Larsimont JC, Sukumaran V, Van de Sande B, Pucci D, Dekoninck S, Berthe JV, Aerts S, Salmon I, Del Marmol V, and Blanpain C.
- Published
- 2012
4. Autonomous Shopping Cart Platform for People with Mobility Impairments
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Marchetti, L., Pucci, D., Morin, Pascal, Visual servoing in robotics, computer vision, and augmented reality (Lagadic), Inria Sophia Antipolis - Méditerranée (CRISAM), Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Inria Rennes – Bretagne Atlantique, Institut National de Recherche en Informatique et en Automatique (Inria)-SIGNAUX ET IMAGES NUMÉRIQUES, ROBOTIQUE (IRISA-D5), Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), CentraleSupélec-Télécom Bretagne-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de Recherche en Informatique et en Automatique (Inria)-École normale supérieure - Rennes (ENS Rennes)-Université de Bretagne Sud (UBS)-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-CentraleSupélec-Télécom Bretagne-Université de Rennes 1 (UR1), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École normale supérieure - Rennes (ENS Rennes)-Université de Bretagne Sud (UBS)-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Advanced Image Understanding and Autonomous Systems (AROLAG), Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Institut des Systèmes Intelligents et de Robotique (ISIR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-Télécom Bretagne-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-Télécom Bretagne-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Télécom Bretagne-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), and Marchand, Eric
- Subjects
[INFO.INFO-RB] Computer Science [cs]/Robotics [cs.RO] ,[INFO.INFO-RB]Computer Science [cs]/Robotics [cs.RO] - Abstract
International audience; Providing a platform able to interact with a spe- cific user is a challenging problem for assistance technologies. Among the many platforms accomplishing this task, we address the problem of designing an autonomous shopping cart. We assume that the shopping cart is set-up on a unicycle-like robot endowed with two sensors: an RGB-D camera and a planar laser range finder. To combine the information from these two sensors, a data fusion algorithm has been developed using a particle filter, augmented with a k-clustering step to extract person estimations. The problem of stabilizing the robot's position at a fixed distance from the user has been solved through classical control design. Results on a real mobile platform verify the effectiveness of the approach here proposed.
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- 2012
5. Responsabilità professionale e gestione del rischio in psichiatria
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Ferracuti, Stefano and Pucci, D.
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incidenti ,sucidio ,responsabilità dell'equipe ,Responsabilità professionale ,colpa - Published
- 2012
6. Immigrazione, traumi, difficoltà vitali e psicopatologia: uno studio preliminare in un setting di medicina generale
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Carrer, S, Meuti, Valentina, Catino, E, Pucci, D, Lafuente, M, Colosimo, F, Aragona, M, Mazzetti, M, Maisano, B, and Geraci, S.
- Published
- 2011
7. Complicanze ostetriche e schizofrenia in uno studio multicentrico in una popolazione italiana
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Bersani, Giuseppe, Pesaresi, L., Pucci, D., Manuali, G., and Amore, M.
- Published
- 2008
8. La stagionalità di nascita nella schizofrenia: incremento nei mesi primaverili e decremento in autunno delle nascite di pazienti schizofrenici
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Pucci, D., Gherardelli, S., Pesaresi, L., Conforti, F., and Bersani, Giuseppe
- Published
- 2007
9. La stagionalità dell'esordio come potenziale indice di sottotipizzazione nel disturbo di panico
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Pesaresi, L., Clemente, R., Limpido, L., Palazzo, L., Pucci, D., and Bersani, Giuseppe
- Published
- 2007
10. Brain abnormalities in schizophrenia. A qualitative comparative study of schizophrenic patients and control individuals assessed by magnetic resonance imaging
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Bersani, Giuseppe, Quartini, A., Piperopoulos, O., Iannitelli, A., Paolemili, M., Pucci, D., DI BIASI, Claudio, Gualdi, G., and Pancheri, Paolo
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Adult ,Male ,Analysis of Variance ,Chi-Square Distribution ,Adolescent, Adult, Analysis of Variance, Case-Control Studies, Cerebral Ventricles ,pathology, Chi-Square Distribution, Humans, Magnetic Resonance Imaging ,methods, Male, Middle Aged, Schizophrenia ,pathology ,Adolescent ,Middle Aged ,Magnetic Resonance Imaging ,methods ,Cerebral Ventricles ,Case-Control Studies ,Schizophrenia ,Humans - Abstract
The present study examined, by means of Magnetic Resonance Imaging (MRI), the qualitative brain abnormalities in a group of 58 schizophrenic patients compared to a group of 58 matched control individuals. The possible relationships between these abnormalities and the demographic and clinical features of the participants in the study were also investigated. Schizophrenic patients presented a higher percentage of bland-moderate enlargement of the periencephalic-subarachnoid spaces (p=0.01) and a widespread cerebral atrophy, the latter below the threshold of significance (p=0.06). In the subset of patients with ventricular asymmetry (right larger than left) the age was significantly lower compared to the age of patients without this abnormality (p=0.04). In the subset of patients with cerebellar cisterns enlargement the age as well as the age of onset was higher in comparison to the one of patients without this abnormality (p=0.02; p=0.006). Taking together with previous studies, these findings underline the importance of qualitative assessment of brain morphology in research and clinical evaluation of patients with schizophrenia.
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- 2006
11. Caratteristiche del profilo circadiano premorboso in soggetti con Disturbi Depressivi
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Russo, D., Fiorentini, S., Pucci, D., Limpido, L., LO GALBO, G., and Bersani, Giuseppe
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- 2006
12. Studio RMN delle alterazioni qualitative cerebrali nella schizofrenia
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Quartini, A., Piperopoulos, O., Iannitelli, A., Paolemili, M., Pucci, D., DI BIASI, C., Gualdi, G., and Bersani, Giuseppe
- Published
- 2005
13. Degradation of mutant p53H175 protein by Zn(II) through autophagy
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Garufi, A, primary, Pucci, D, additional, D’Orazi, V, additional, Cirone, M, additional, Bossi, G, additional, Avantaggiati, M L, additional, and D’Orazi, G, additional
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- 2014
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14. Crystallization and co-crystallization of ZnIIheteroleptic complexes: modulation of properties
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Crispini, A., primary, Pucci, D., additional, Szerb, E., additional, Mastropietro, T., additional, Talarico, A. M., additional, Sanz, B., additional, and Ghedini, M., additional
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- 2012
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15. DIELECTRIC RELAXATION INVESTIGATIONS OF NEW SYNTHETIZED ORGANO-METALLIC LIQUID CRYSTALS
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Beica, T., ALEXE IONESCU, A. L., Ionescu, A. T., Miraldi, Elio, Pucci, D., and Rajteri, M.
- Published
- 1995
16. The 'organic fluorine' in action in the construction of organometallic molecular materials
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Crispini, A., primary, Aiello, I., additional, Ghedini, M., additional, Mastropietro, T., additional, Aprea, A., additional, Pucci, D., additional, Deda, M. L., additional, Amati, M., additional, and Lelj, F., additional
- Published
- 2011
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17. Adult Cancer Survivorship: Evolution, Research, and Planning Care
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Jacobs, L. A., primary, Palmer, S. C., additional, Schwartz, L. A., additional, DeMichele, A., additional, Mao, J. J., additional, Carver, J., additional, Gracia, C., additional, Hill-Kayser, C. E., additional, Metz, J. M., additional, Hampshire, M. K., additional, Vachani, C., additional, Pucci, D., additional, and Meadows, A. T., additional
- Published
- 2009
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18. trans-2,2'-Dichloro-4,4'-dimethylazobenzene
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Crispini, A., primary, Ghedini, M., additional, and Pucci, D., additional
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- 1998
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19. Paraplegic Gait Programs
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Pucci, D, primary and Skopek, K., additional
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- 1994
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20. Low placental growth factor across pregnancy identifies a subset of women with preterm preeclampsia: type 1 versus type 2 preeclampsia?
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Powers RW, Roberts JM, Plymire DA, Pucci D, Datwyler SA, Laird DM, Sogin DC, Jeyabalan A, Hubel CA, Gandley RE, Powers, Robert W, Roberts, James M, Plymire, Daniel A, Pucci, Dominick, Datwyler, Saul A, Laird, Don M, Sogin, David C, Jeyabalan, Arun, Hubel, Carl A, and Gandley, Robin E
- Abstract
Preeclampsia is a heterogeneous syndrome affecting 3% to 5% of all pregnancies. An imbalance of the antiangiogenic and proangiogenic factors, soluble receptor fms-like tyrosine kinase 1 and placental growth factor (PGF), is thought to contribute to the pathophysiology of preeclampsia. Maternal plasma PGF and soluble receptor fms-like tyrosine kinase 1 were quantified by specific immunoassays in cross-sectional samples from 130 preeclamptic subjects and 342 normotensive controls at delivery and longitudinally in samples from 50 women who developed preeclampsia and 250 normotensive controls. Among women who developed preeclampsia, 46% (n=23) evidenced a pattern of consistently low maternal PGF across pregnancy below the lower 95% CI of controls from 15 weeks' gestation to term. In contrast, the remaining 54% (n=27) of women who developed preeclampsia had maternal PGF concentrations similar to or above (n=7) those of normotensive controls. Subjects with low PGF across pregnancy who developed preeclampsia evidenced significantly higher blood pressure in early pregnancy (P<0.05) and, after diagnosis, earlier gestational age at delivery (P<0.05) and more preterm birth (P<0.05) compared with preeclamptic patients with high PGF. A significant subset of women who develop preeclampsia show evidence of consistently low PGF across pregnancy. Low PGF with preeclampsia was associated with preterm delivery compared with preeclamptic patients with high PGF. Identifying women with consistently low plasma PGF during pregnancy may provide a greater understanding of preeclampsia pathophysiology and may provide more focused research and clinical activities. [ABSTRACT FROM AUTHOR]
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- 2012
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21. Feasibility trial of electroacupuncture for aromatase inhibitor-related arthralgia in breast cancer survivors.
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Mao JJ, Bruner DW, Stricker C, Farrar JT, Xie SX, Bowman MA, Pucci D, Han X, and DeMichele A
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- 2009
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22. Rehabilitation activities in a psychiatry emergency unit spdc di monterotondo (rm) dsmdp asl roma 5.
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Mari, L., Forte, A., Brunelleschi, M., De Rossi, P., Pucci, D., Gubbini, S., De Luca, V., Pallottino, S., Madia, P., Bianciardi, E., Manzi, A., and Nicolò, G.
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PSYCHIATRIC emergencies ,SOCIAL skills education ,COGNITIVE remediation ,CLIENT satisfaction ,STRESS management ,EVIDENCE-based psychiatry - Abstract
Introduction: Psychiatric rehabilitation activities (PRA) aim at restoring community functioning and improving well-being and quality of life of patients, guiding them towards independence and taking responsibility for their own existence. PRA suitable to psychiatry emergency settings are crucial as they prepare effective postdischarge therapeutic programs. Our psychiatry emergency unit designed rapid group PRA based on a combination of personcentred, evidence-based interventions. Objectives: The aim of our study is to assess several aspects of our PRA: participation rate, degree of participants' satisfaction, perceived utility, and the quality of climate within the group. Methods: Self-report questionnaires were administered in our psychiatry emergency unit to acute patients during their hospitalization. Patients underwent our PRA, a structured weekly program based on a combination of cognitive-behavioural interventions such as Psycho-education, Cognitive Remediation and Social Skills Training. Results: From April to December 2018, 195 self-report questionnaires were administered. 88.5% of participants described PRA as very useful to their recovery process, 87% considered themselves very satisfied with the climate within the group and 86.1% of the sample was very satisfied with the quality of time spent on PRA. Conclusions: Monitoring our group program resulted in a positive feedback on PRA, with high rates of perceived efficacy and satisfaction. High acceptability makes this intervention a promising approach able to promote awareness and recovery in acute patients. The therapeutic effects of PRA possibly unfold through the enhanced ability to cope with stress and to manage personal vulnerabilities, along with an improvement of cognitive functions. [ABSTRACT FROM AUTHOR]
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- 2020
23. Two-wavelength operation of a double-pass planar dye laser amplifier
- Author
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Burlamacchi, P., Coisson, R., Pratesi, R., and Pucci, D.
- Abstract
The simultaneous multiple line operation of a double pass dye laser amplifier is reported. Standard schemes have been used to generate two independently tunable lines and to scan one narrow line linearly around a fixed one. A simple, new scheme is illustrated, which allows symmetric splitting of narrow laser lines.
- Published
- 1977
24. ATP-citrate lyase phosphorylation in rat adipose tissue.
- Author
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Pucci, D L, Ramakrishna, S, and Benjamin, W B
- Abstract
Previous in vitro studies demonstrated that ATP-citrate lyase is phosphorylated by cyclic AMP-dependent protein kinase at peptide A, containing a phosphoserine residue, and by ATP-citrate lyase kinase at peptide B, containing both phosphoserine and phosphothreonine residues (Ramakrishna, S., Pucci, D. L., and Benjamin, W. B. (1983) J. Biol. Chem. 258, 4950-4956). In the present study, trypsin-digested, radiolabeled ATP-citrate lyase from rat epididymal fat pads was analyzed by high performance liquid chromatography. Phosphorylation occurred at three amino acid residues within two different peptide sequences; one (peptide a) contained phosphoserine and the other (peptide b) contained phosphoserine and phosphothreonine. The retention times and molecular weights were the same for peptides a and A and peptides b and B. Isoproterenol action increased peptide a phosphorylation and, to a lesser extent, peptide b phosphorylation. Insulin action also increased peptide a phosphorylation, but did not increase peptide b phosphorylation.
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- 1983
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25. Dependence of ATP-citrate lyase kinase activity on the phosphorylation of ATP-citrate lyase by cyclic AMP-dependent protein kinase.
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Ramakrishna, S, Pucci, D L, and Benjamin, W B
- Abstract
ATP-citrate lyase from rat liver and adipose tissue is phosphorylated by either ATP-citrate lyase kinase or catalytic subunit of cyclic AMP-dependent protein kinase to 0.5-0.6 mol/subunit. We previously demonstrated that the site phosphorylated by ATP-citrate lyase kinase (peptide B) is different from that phosphorylated by catalytic subunit of cyclic AMP-dependent protein kinase (peptide A) (Ramakrishna, S., Pucci, D. L., and Benjamin, W.B. (1981) J. Biol. Chem. 256, 10213-10216). ATP-citrate lyase phosphorylation by both protein kinases added simultaneously was increased synergistically. When ATP-citrate lyase was first phosphorylated by catalytic subunit of cyclic AMP-dependent protein kinase, the net phosphorylation of the fragments subsequently phosphorylated by lyase kinase increased about 6-fold. However, when ATP-citrate lyase was first phosphorylated by lyase kinase, there was no effect on the subsequent phosphorylation of the enzyme by cyclic AMP-dependent protein kinase. Alkaline phosphatase-dephosphorylated ATP-citrate lyase was phosphorylated by catalytic subunit of cyclic AMP-dependent protein kinase to 0.9-1.0 mol/subunit. However, dephospho-ATP-citrate lyase was not phosphorylated by lyase kinase. The addition of both protein kinases simultaneously phosphorylated ATP-citrate lyase up to 2 mol/subunit. Phosphorylation of dephospho-ATP-citrate lyase first by catalytic subunit of cyclic AMP-dependent protein kinase and ATP enabled the lyase to be phosphorylated by lyase kinase. Peptide mapping and phosphoamino acid analysis of dephospho-ATP-citrate lyase phosphorylated by catalytic subunit of cyclic AMP-dependent protein kinase and/or lyase kinase conclusively showed that phosphorylation of ATP-citrate lyase by ATP-citrate lyase kinase was completely dependent on peptide A phosphorylation by cyclic AMP-dependent protein kinase. Furthermore, increased phosphorylation when both protein kinases were added simultaneously was due to increased phosphorylation at peptide B.
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- 1983
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26. Un programma metodologicamente avanzato per la rilevazione della qualità percepita - lo studio EUSAM (Esperienze degli Utenti in Salute Mentale)
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Gigantesco, A., Tarolla, E., Del Re, D., Cascavilla, I., Orso, L., Dario, C., Luciani, A., Chiaia, E., Andronico, F., Oggiano F, Pacifico G., Caviglia, B., Rivano, O., Veltro, F., Iannone, C., Stefano Ferracuti, Pucci, D., Cicchetti, R., Franzò, G., Mascioli, M., Savino, A., and Triggiani, A.
27. Palladium(II) complexes with the cyclometallated C-Y-N(sp^2)-Pd-C(sp^2) ring (Y = N,C) and (R)-(+)-hydroxy-methylene camphor
- Author
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Ghedini, M., Neve, F., Pucci, D., and Cesarotti, E.
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- 1992
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28. A Novel Approach for an Integrated Straw tube-Microstrip Detector
- Author
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E. Basile, F. Bellucci, L. Benussi, M. Bertani, S. Bianco, M.A. Caponero, D. Colonna, F. Di Falco, F.L. Fabbri, F. Felli, M. Giardoni, A. La Monaca, G. Mensitieri, B. Ortenzi, M. Pallotta, A. Paolozzi, L. Passamonti, D. Pierluigi, C. Pucci, A. Russo, G. Saviano, F. Casali, M. Bettuzzi, D. Bianconi, F. Baruffaldi, E. Perilli, F. Massa, E. b., Basile, Bellucci, Francesco, L., Benussi, M., Bertani, S., Bianco, M. d., Caponero, D. b., Colonna, F. b., Di, F., Fabbri, F. b., Felli, M., Giardoni, A. L., Monaca, G. c., Mensitieri, B., Ortenzi, M., Pallotta, A. b., Paolozzi, L., Passamonti, D., Pierluigi, C. b., Pucci, A., Russo, G. b., Saviano, F., Casali, M., Bettuzzi, D., Bianconi, F., Baruffaldi, E., Perilli, F., Massa, Basile E., Bellucci F., Benussi L., Bertani M., Bianco S., Caponero M.A., Colonna D., Di Falco F., Fabbri F.L., Felli F., Giardoni M., La Monaca A., Mensitieri G., Ortenzi B., Pallotta M., Paolozzi A., Passamonti L., Pierluigi D., Pucci D., Russo A., Saviano G., Casali F., Bettuzzi M., Bianconi D., Baruffaldi F., Perilli E., and Massa F.
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Straw tube ,Nuclear and High Energy Physics ,Materials science ,Physics - Instrumentation and Detectors ,Silicon ,Mechanical tension, Silicon microstrips, Test beam performances, Tracking ,Detector ,elementary particles ,chemistry.chemical_element ,FOS: Physical sciences ,Instrumentation and Detectors (physics.ins-det) ,Fibre-reinforced plastic ,Straw ,tracking ,Microstrip devices ,Mechanical tension ,Carbon fiber reinforced plastics, Crystal lattices, Elementary particles, Mechanical properties, Performance, Radiation detectors, Tensile testing ,Nuclear Energy and Engineering ,Test beam ,chemistry ,Electrical and Electronic Engineering ,Composite material ,Silicon microstrip detectors ,detectors - Abstract
We report on a novel concept of silicon microstrips and straw tubes detector, where integration is accomplished by a straw module with straws not subjected to mechanical tension in a Rohacell $^{\circledR}$ lattice and carbon fiber reinforced plastic shell. Results on mechanical and test beam performances are reported on as well., Accepted by Transactions on Nuclear Science (2005). 11 pages, 9 figures, uses lnfprep.sty
- Published
- 2005
29. Modeling and Calibration of Pressure-Sensing Insoles via a New Plenum-Based Chamber.
- Author
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Belli I, Sorrentino I, Dussoni S, Milani G, Rapetti L, Tirupachuri Y, Valli E, Vanteddu PR, Maggiali M, and Pucci D
- Abstract
This paper proposes a novel method to reliably calibrate a pair of sensorized insoles utilizing an array of capacitive tactile pixels ( taxels ). A new calibration setup is introduced that is scalable and suitable for multiple kinds of wearable sensors and a procedure for the simultaneous calibration of each of the sensors in the insoles is presented. The calibration relies on a two-step optimization algorithm that, firstly, enables determination of a relevant set of mathematical models based on the instantaneous measurement of the taxels alone, and, then, expands these models to include the relevant portion of the time history of the system. By comparing the resulting models with our previous work on the same hardware, we demonstrate the effectiveness of the novel method both in terms of increased ability to cope with the non-linear characteristics of the sensors and increased pressure ranges achieved during the experiments performed.
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- 2023
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30. Psychological Characteristics of Women with Perinatal Depression Who Require Psychiatric Support during Pregnancy or Postpartum: A Cross-Sectional Study.
- Author
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Terrone G, Bianciardi E, Fontana A, Pinci C, Castellani G, Sferra I, Forastiere A, Merlo M, Marinucci E, Rinaldi F, Falanga M, Pucci D, Siracusano A, and Niolu C
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- Female, Pregnancy, Humans, Cross-Sectional Studies, Postpartum Period psychology, Parturition, Anxiety psychology, Risk Factors, Depression epidemiology, Depression psychology, Depression, Postpartum epidemiology, Depression, Postpartum psychology
- Abstract
Antenatal depression may be distinct from postpartum depression in terms of prevalence, severity of symptoms, comorbidities, prognosis, and risk factors. Although risk factors for perinatal depression have been identified, it is unclear whether there are differences in the onset of perinatal depression (PND). This study explored the characteristics of women requiring mental health support during pregnancy or postpartum. A sample of 170 women (58% in pregnancy; 42% postpartum) who contacted the SOS-MAMMA outpatient clinic was recruited. Clinical data sheets and self-report questionnaires (EPDS, LTE-Q, BIG FIVE; ECR; BSQ; STICSA) were administered, hypothesizing possible risk factors, such as personality traits, stressful life events, body dissatisfaction, attachment style, and anxiety. Hierarchical regression models were carried out in the pregnancy (F
10;36 = 8.075, p < 0.001, adjR2 = 0.877) and postpartum groups (F10;38 = 3.082, p < 0.05, adjR2 = 0.809). Recent stressful life events and conscientiousness were associated with depression in both the pregnant (29.3%, 25.5% of variance) and postpartum groups (23.8%, 20.7% of variance). In pregnant women, "openness" (11.6%), body dissatisfaction (10.2%), and anxiety (7.1%) symptoms were predictive of depression. In the postpartum group, "neuroticism" (13.8%) and insecure romantic attachment dimensions (13.4%; 9.2%) were the strongest predictors. Perinatal psychological interventions should consider the differences between mothers with depression during pregnancy and postpartum.- Published
- 2023
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31. Joint-Based Action Progress Prediction.
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Pucci D, Becattini F, and Del Bimbo A
- Abstract
Action understanding is a fundamental computer vision branch for several applications, ranging from surveillance to robotics. Most works deal with localizing and recognizing the action in both time and space, without providing a characterization of its evolution. Recent works have addressed the prediction of action progress, which is an estimate of how far the action has advanced as it is performed. In this paper, we propose to predict action progress using a different modality compared to previous methods: body joints. Human body joints carry very precise information about human poses, which we believe are a much more lightweight and effective way of characterizing actions and therefore their execution. Estimating action progress can in fact be determined based on the understanding of how key poses follow each other during the development of an activity. We show how an action progress prediction model can exploit body joints and integrate it with modules providing keypoint and action information in order to be run directly from raw pixels. The proposed method is experimentally validated on the Penn Action Dataset.
- Published
- 2023
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32. Salivary cotinine level and treatment response in muscle invasive bladder cancer: A pilot study.
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Gotschall JW, Major CK, Jacobs LA, Blauch A, Pucci D, Palmer SC, and Mamtani R
- Abstract
Competing Interests: Dr. Mamtani reported receiving honoraria from Flatiron Health and research funding from Merck. He also served in an advisory role for Genetech/Roche and Seattle Genetics/Astellas. No other disclosures were reported.
- Published
- 2022
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33. Assessing the efficiency of exoskeletons in physical strain reduction by biomechanical simulation with AnyBody Modeling System.
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Fritzsche L, Galibarov PE, Gärtner C, Bornmann J, Damsgaard M, Wall R, Schirrmeister B, Gonzalez-Vargas J, Pucci D, Maurice P, Ivaldi S, and Babič J
- Abstract
Introduction: Recently, many industrial exoskeletons for supporting workers in heavy physical tasks have been developed. However, the efficiency of exoskeletons with regard to physical strain reduction has not been fully proved, yet. Several laboratory and field studies have been conducted, but still more data, that cannot be obtained solely by behavioral experiments, are needed to investigate effects on the human body., Methods: This paper presents an approach to extend laboratory and field research with biomechanical simulations using the AnyBody Modeling System. Based on a dataset recorded in a laboratory experiment with 12 participants using the exoskeleton Paexo Shoulder in an overhead task, the same situation was reproduced in a virtual environment and analyzed with biomechanical simulation., Results: Simulation results indicate that the exoskeleton substantially reduces muscle activity and joint reaction forces in relevant body areas. Deltoid muscle activity and glenohumeral joint forces in the shoulder were decreased between 54 and 87%. Simultanously, no increases of muscle activity and forces in other body areas were observed., Discussion: This study demonstrates how a simulation framework could be used to evaluate changes in internal body loads as a result of wearing exoskeletons. Biomechanical simulation results widely agree with experimental measurements in the previous laboratory experiment and supplement such by providing an insight into effects on the human musculoskeletal system. They confirm that Paexo Shoulder is an effective device to reduce physical strain in overhead tasks. The framework can be extended with further parameters, allowing investigations for product design and evaluation., Competing Interests: Authors state no conflict of interests. Some authors work for Ottobock SE & Co. KGaA, a manufacturer of industrial exoskeletons., (© The Author(s) 2021.)
- Published
- 2021
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34. Editorial: Bridging the Gap Between the Lab and the Real World: Future Perspectives for Legged Robots.
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Focchi M, Pucci D, and Del Prete A
- Abstract
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
- Published
- 2020
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35. Learning to Avoid Obstacles With Minimal Intervention Control.
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Duan A, Camoriano R, Ferigo D, Huang Y, Calandriello D, Rosasco L, and Pucci D
- Abstract
Programming by demonstration has received much attention as it offers a general framework which allows robots to efficiently acquire novel motor skills from a human teacher. While traditional imitation learning that only focuses on either Cartesian or joint space might become inappropriate in situations where both spaces are equally important (e.g., writing or striking task), hybrid imitation learning of skills in both Cartesian and joint spaces simultaneously has been studied recently. However, an important issue which often arises in dynamical or unstructured environments is overlooked, namely how can a robot avoid obstacles? In this paper, we aim to address the problem of avoiding obstacles in the context of hybrid imitation learning. Specifically, we propose to tackle three subproblems: (i) designing a proper potential field so as to bypass obstacles, (ii) guaranteeing joint limits are respected when adjusting trajectories in the process of avoiding obstacles, and (iii) determining proper control commands for robots such that potential human-robot interaction is safe. By solving the aforementioned subproblems, the robot is capable of generalizing observed skills to new situations featuring obstacles in a feasible and safe manner. The effectiveness of the proposed method is validated through a toy example as well as a real transportation experiment on the iCub humanoid robot., (Copyright © 2020 Duan, Camoriano, Ferigo, Huang, Calandriello, Rosasco and Pucci.)
- Published
- 2020
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36. A Novel Sensorised Insole for Sensing Feet Pressure Distributions.
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Sorrentino I, Andrade Chavez FJ, Latella C, Fiorio L, Traversaro S, Rapetti L, Tirupachuri Y, Guedelha N, Maggiali M, Dussoni S, Metta G, and Pucci D
- Subjects
- Biomechanical Phenomena, Foot Orthoses, Humans, Pressure, Wearable Electronic Devices, Biosensing Techniques, Foot physiology, Gait physiology, Touch physiology
- Abstract
Wearable sensors are gaining in popularity because they enable outdoor experimental monitoring. This paper presents a cost-effective sensorised insole based on a mesh of tactile capacitive sensors. Each sensor's spatial resolution is about 4 taxels/cm 2 in order to have an accurate reconstruction of the contact pressure distribution. As a consequence, the insole provides information such as contact forces, moments, and centre of pressure. To retrieve this information, a calibration technique that fuses measurements from a vacuum chamber and shoes equipped with force/torque sensors is proposed. The validation analysis shows that the best performance achieved a root mean square error (RMSE) of about 7 N for the contact forces and 2 N m for the contact moments when using the force/torque shoe data as ground truth. Thus, the insole may be an alternative to force/torque sensors for certain applications, with a considerably more cost-effective and less invasive hardware.
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- 2020
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37. Objective and Subjective Effects of a Passive Exoskeleton on Overhead Work.
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Maurice P, Camernik J, Gorjan D, Schirrmeister B, Bornmann J, Tagliapietra L, Latella C, Pucci D, Fritzsche L, Ivaldi S, and Babic J
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- Arm, Arm Injuries prevention & control, Biomechanical Phenomena, Electromyography, Healthy Volunteers, Humans, Male, Neurophysiological Monitoring, Patient Acceptance of Health Care, Psychomotor Performance, Workload, Young Adult, Exoskeleton Device adverse effects, Musculoskeletal Diseases rehabilitation, Prosthesis Design, Upper Extremity
- Abstract
Overhead work is a frequent cause of shoulder work-related musculoskeletal disorders. Exoskeletons offering arm support have the potential to reduce shoulder strain, without requiring large scale reorganization of the workspace. Assessment of such systems however requires to take multiple factors into consideration. This paper presents a thorough in-lab assessment of PAEXO, a novel passive exoskeleton for arm support during overhead work. A list of evaluation criteria and associated performance metrics is proposed to cover both objective and subjective effects of the exoskeleton, on the user and on the task being performed. These metrics are measured during a lab study, where 12 participants perform an overhead pointing task with and without the exoskeleton, while their physical, physiological and psychological states are monitored. Results show that using PAEXO reduces shoulder physical strain as well as global physiological strain, without increasing low back strain nor degrading balance. These positive effects are achieved without degrading task performance. Importantly, participants' opinions of PAEXO are positive, in agreement with the objective measures. Thus, PAEXO seems a promising solution to help prevent shoulder injuries and diseases among overhead workers, without negatively impacting productivity.
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- 2020
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38. Six-Axis Force Torque Sensor Model-Based In Situ Calibration Method and Its Impact in Floating-Based Robot Dynamic Performance.
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Andrade Chavez FJ, Traversaro S, and Pucci D
- Abstract
A crucial part of dynamic motions is the interaction with other objects or the environment. Floating base robots have yet to perform these motions repeatably and reliably. Force torque sensors are able to provide the full description of a contact. Despite that, their use beyond a simple threshold logic is not widespread in floating base robots. Force torque sensors might change performance when mounted, which is why in situ calibration methods can improve the performance of robots by ensuring better force torque measurements. The Model-Based in situ calibration method with temperature compensation has shown promising results in improving FT sensor measurements. There are two main goals for this paper. The first is to facilitate the use and understanding of the method by providing guidelines that show their usefulness through experimental results. Then the impact of having better FT measurements with no temperature drift are demonstrated by proving that the offset estimated with this method is still useful days and even a month from the time of estimation. The effect of this is showcased by comparing the sensor response with different offsets simultaneously during real robot experiments. Furthermore, quantitative results of the improvement in dynamic behaviors due to the in situ calibration are shown. Finally, we show how using better FT measurements as feedback in low and high level controllers can impact the performance of floating base robots during dynamic motions. Experiments were performed on the floating base robot iCub.
- Published
- 2019
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39. Simultaneous Floating-Base Estimation of Human Kinematics and Joint Torques.
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Latella C, Traversaro S, Ferigo D, Tirupachuri Y, Rapetti L, Andrade Chavez FJ, Nori F, and Pucci D
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- Biomechanical Phenomena, Humans, Models, Theoretical, Motion, Shoes, Torque, Wearable Electronic Devices, Joints physiology, Models, Biological, Monitoring, Physiologic methods, Walking physiology
- Abstract
The paper presents a stochastic methodology for the simultaneous floating-base estimation of the human whole-body kinematics and dynamics (i.e., joint torques, internal and external forces). The paper builds upon our former work where a fixed-base formulation had been developed for the human estimation problem. The presented approach is validated by presenting experimental results of a health subject equipped with a wearable motion tracking system and a pair of shoes sensorized with force/torque sensors while performing different motion tasks, e.g., walking on a treadmill. The results show that joint torque estimates obtained by using floating-base and fixed-base approaches match satisfactorily, thus validating the present approach.
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- 2019
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40. Does the efficacy of asenapine in bipolar disorder increase in the presence of comorbidity with a substance use disorder? A naturalistic study.
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De Filippis S, Cuomo I, Kotzalidis GD, Pucci D, Zingaretti P, Porrari R, Fini C, Motta P, Caloro M, and Girardi P
- Abstract
Background: Asenapine is a second-generation antipsychotic approved in Europe for treating moderate-to-severe manic episodes in adults affected by type I bipolar disorder (BD-I). We aimed to compare its efficacy in psychiatric inpatients with BD-I, with or without substance use disorder (SUD)., Methods: We administered flexible asenapine doses ranging from 5-20 mg/day to 119 voluntarily hospitalized patients with Diagnostic and Statistical Manual of Mental Disorders, 4th Edition, Text Revision (DSM-IV-TR) BD-I diagnosis, with or without SUD. Patients were assessed with clinician-rated questionnaires [i.e. Brief Psychiatric Rating Scale (BPRS), Young Mania Rating Scale (YMRS), Hamilton Depression Rating Scale (HDRS), Hamilton Anxiety Rating Scale (HARS), and Global Assessment of Functioning (GAF)]. Assessments were carried out at baseline (T0, prior to treatment), and 3 (T1), 7 (T2), 15 (T3), and 30 days (T4) after starting treatment for all clinical scales and at T0 and T4 for the GAF., Results: Patients improved on all scales ( p < 0.001) across all timepoints, as shown both by paired-sample comparisons and by applying a repeated-measures, generalized linear model (GLM). Patients without comorbid SUD showed greater reductions in BPRS scores at T2 and T3, greater reduction in YMRS scores at T3, and lower HARS scores at all timepoints. HDRS scores did not differ between the two groups at any timepoint. However, the reduction in HARS scores in the comorbid group was stronger than in the BD-I only group, albeit not significantly. Side effects were few and mild-to-moderate., Conclusions: The open-label design and the relatively short observation period may expose to both type I and type II statistical errors (false positive and false negatives). Asenapine showed effectiveness and safety in hospitalized BD-I patients. Its effect was stronger in patients without comorbid SUD., Competing Interests: Conflict of interest statement: The authors declare that there is no conflict of interest.
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- 2017
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41. A fluorescent curcumin-based Zn(II)-complex reactivates mutant (R175H and R273H) p53 in cancer cells.
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Garufi A, Trisciuoglio D, Porru M, Leonetti C, Stoppacciaro A, D'Orazi V, Avantaggiati M, Crispini A, Pucci D, and D'Orazi G
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- Animals, Brain Neoplasms genetics, Cations, Divalent chemistry, Cations, Divalent pharmacology, Cell Death, Cell Line, Tumor, Coordination Complexes chemistry, Disease Models, Animal, Gene Expression, Glioblastoma genetics, Humans, Mice, Mice, Nude, Mutation, Protein Conformation, Random Allocation, Tumor Suppressor Protein p53 genetics, Tumor Suppressor Protein p53 metabolism, Brain Neoplasms metabolism, Coordination Complexes pharmacology, Curcumin analogs & derivatives, Curcumin pharmacology, Glioblastoma metabolism, Zinc chemistry, Zinc pharmacology
- Abstract
Background: Mutations of the p53 oncosuppressor gene are amongst the most frequent aberration seen in human cancer. Some mutant (mt) p53 proteins are prone to loss of Zn(II) ion that is bound to the wild-type (wt) core, promoting protein aggregation and therefore unfolding. Misfolded p53 protein conformation impairs wtp53-DNA binding and transactivation activities, favouring tumor growth and resistance to antitumor therapies. Screening studies, devoted to identify small molecules that reactivate mtp53, represent therefore an attractive anti-cancer therapeutic strategy. Here we tested a novel fluorescent curcumin-based Zn(II)-complex (Zn-curc) to evaluate its effect on mtp53 reactivation in cancer cells., Methods: P53 protein conformation was examined after Zn-curc treatment by immunoprecipitation and immunofluorescence assays, using conformation-specific antibodies. The mtp53 reactivation was evaluated by chromatin-immunoprecipitation (ChIP) and semi-quantitative RT-PCR analyses of wild-type p53 target genes. The intratumoral Zn-curc localization was evaluated by immunofluorescence analysis of glioblastoma tissues of an ortothopic mice model., Results: The Zn-curc complex induced conformational change in p53-R175H and -R273H mutant proteins, two of the most common p53 mutations. Zn-curc treatment restored wtp53-DNA binding and transactivation functions and induced apoptotic cell death. In vivo studies showed that the Zn-curc complex reached glioblastoma tissues of an ortothopic mice model, highlighting its ability to crossed the blood-tumor barrier., Conclusions: Our results demonstrate that Zn-curc complex may reactivate specific mtp53 proteins and that may cross the blood-tumor barrier, becoming a promising compound for the development of drugs to halt tumor growth.
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- 2013
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42. Traumatic events, post-migration living difficulties and post-traumatic symptoms in first generation immigrants: a primary care study.
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Aragona M, Pucci D, Mazzetti M, Maisano B, and Geraci S
- Subjects
- Adolescent, Adult, Aged, Ambulatory Care Facilities, Anxiety psychology, Depression psychology, Educational Status, Female, Humans, Italy epidemiology, Male, Middle Aged, Primary Health Care, Risk, Socioeconomic Factors, Stress Disorders, Post-Traumatic epidemiology, Surveys and Questionnaires, Young Adult, Emigrants and Immigrants psychology, Emigration and Immigration statistics & numerical data, Stress Disorders, Post-Traumatic psychology, Wounds and Injuries psychology
- Abstract
Objectives: To study potentially traumatic events (PTE), post-traumatic stress disorder (PTSD), anxiety, depression, somatization and post-migration living difficulties (PMLD) in primary care immigrants., Design: Patients self-rated transculturally validated questionnaires. Those with and without PTSD were compared on all variables. The influence of the number of PTE and of PMLD on PTSD was measured., Results: 391 patients completed the questionnaires. Prevalence of PTSD was 10.2%. PTE and PMLD were frequent in the whole sample but more common in PTSD subjects. Either the number of PTE and of PMLD significantly increased the likelihood to have a PTSD., Conclusions: PTE, PMLD, PTSD and related conditions (anxiety, depression and somatization) are frequent among immigrants in primary care, and either PTE and PMLD significantly influence resulting psychopathology. The implications in clinical practice are discussed.
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- 2013
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43. The role of post-migration living difficulties on somatization among first-generation immigrants visited in a primary care service.
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Aragona M, Pucci D, Carrer S, Catino E, Tomaselli A, Colosimo F, Lafuente M, Mazzetti M, Maisano B, and Geraci S
- Subjects
- Adult, Female, Humans, Italy epidemiology, Male, Middle Aged, Social Conditions, Socioeconomic Factors, Stress Disorders, Post-Traumatic epidemiology, Stress Disorders, Post-Traumatic psychology, Surveys and Questionnaires, Primary Health Care statistics & numerical data, Somatoform Disorders epidemiology, Somatoform Disorders psychology, Transients and Migrants psychology
- Abstract
The role of post-migration living difficulties (PMLD) on somatization was studied in 101 first generation immigrants visited in primary care. Premigratory traumas and post-traumatic stress disorder (PTSD) were also assessed. About one third of patients somatized. Sociodemographic variables were similar in somatizers and non-somatizers. Premigratory traumas, PTSD and the likelihood to report at least one serious or very serious PMLD were higher in somatizers. Four kinds of PMLD were more frequent in somatizers: worries about unavailability of health assistance, working problems, discrimination and poor social help. Traumas and PTSD influenced the effect of PMLD on somatization. Findings suggest that in specific samples of primary care immigrants severe premigratory traumas increase the sensitivity to PMLD and in turn distress due to PMLD amplifies the tendency to somatize.
- Published
- 2011
- Full Text
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44. Functional properties of metallomesogens modulated by molecular and supramolecular exotic arrangements.
- Author
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Crispini A, Ghedini M, and Pucci D
- Abstract
New concepts for the synthesis of metallomesogens have been recently developed in order to use the metal centre as a scaffold for grafting different functionalities and inducing non-conventional shapes and properties in the resulting complexes. Our strategy was based on the synthesis of mesogenic coordination complexes whose molecular architectures are controlled by the modulation of different and tunable molecular motifs: the nature of the metal ion and the surrounding ligands as central unit, the number of flexible chains at the periphery, and the nature of counter-ions in ionic complexes. The appropriate choice of molecular construction motifs allows control at global architectures and induces pre-selected properties from the level of single molecule to supramolecular network, confirming that metal coordination provides a helpful tool for obtaining multifunctional soft materials.
- Published
- 2009
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45. Development of fully automated determination of marker-specific immunoglobulin G (IgG) avidity based on the avidity competition assay format: application for Abbott Architect cytomegalovirus and Toxo IgG Avidity assays.
- Author
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Curdt I, Praast G, Sickinger E, Schultess J, Herold I, Braun HB, Bernhardt S, Maine GT, Smith DD, Hsu S, Christ HM, Pucci D, Hausmann M, and Herzogenrath J
- Subjects
- Animals, Automation, Cytomegalovirus immunology, Cytomegalovirus Infections immunology, Humans, Immunoglobulin M immunology, Sensitivity and Specificity, Toxoplasma immunology, Toxoplasmosis immunology, Antibodies, Protozoan immunology, Antibodies, Viral immunology, Antibody Affinity, Cytomegalovirus Infections diagnosis, Immunoglobulin G immunology, Toxoplasmosis diagnosis
- Abstract
Determination of the avidity of immunoglobulin G (IgG) directed against a specific marker has become an established diagnostic tool for identifying or excluding acute infections with pathogens. A novel assay format termed AVIcomp (avidity competition based on mass action) circumventing the conventional chaotropic format has been developed for determination of the avidity of marker-specific IgG in patient specimens. Its applications for cytomegalovirus (CMV) and Toxoplasma gondii are presented. Specific high-avidity IgG from the patient specimen is selectively blocked using a soluble antigen in a sample pretreatment reagent, and the amount of remaining specific low-avidity IgG is determined relative to that in an untreated control. The comparison of the conventional chaotropic format, represented by the Radim CMV IgG Avidity assay, and the newly developed AVIcomp method, as exemplified by the Architect CMV IgG Avidity assay, on blood drawn within 4 months after seroconversion revealed a sensitivity of 100% (97.3% by an alternative calculation) for the AVIcomp format versus 87.5% (75.7% by an alternative calculation) for the chaotropic avidity assay. The specificity on 312 CMV IgG reactive and CMV IgM nonreactive specimens from pregnant women was 100% for the AVIcomp assay and 99.7% for the conventional avidity assay. The Architect Toxo IgG Avidity assay showed an agreement of 97.2% with the bioMérieux Vidas Toxo IgG Avidity Assay employing chaotropic reagents. These performance data suggest that the AVIcomp format shows superior sensitivity and equivalent specificity for the determination of IgG avidity to assays based on the chaotropic method and that the AVIcomp format may also be applicable to other disease states.
- Published
- 2009
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- View/download PDF
46. Employee thoughts on influenza vaccine: here we go again.
- Author
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Chan-Tompkins NH, Sahud A, Pucci D, and Herbert C
- Subjects
- Data Collection, Humans, Influenza, Human psychology, Attitude of Health Personnel, Immunization Programs statistics & numerical data, Influenza Vaccines administration & dosage, Influenza, Human prevention & control, Occupational Health Services statistics & numerical data
- Published
- 2008
- Full Text
- View/download PDF
47. Characteristics of HCV positive patients in an Italian urban psychiatric unit.
- Author
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Raja M, Azzoni A, and Pucci D
- Abstract
Objectives: 1) to assess the prevalence of hepatitis C virus (HCV) infection in a population of acute psychiatric in-patients; 2) to find out relationships between HCV comorbidity and clinical features of psychiatric patients., Methods: Prospective observational study in a 6-year period., Results: 2396 cases (1492 patients) were admitted in the considered period. Forty-two patients (2.8%) were affected by HCV infection. HCV infection was more frequent in patients with less years of education, lower social class, lower last year best Global Assessment of Functioning score, more hostile or violent behavior in hospital, with a lifetime history of previous suicide attempt, and with substance-related disorders., Conclusion: HCV infection in psychiatric patients constitutes a major threat to the health of psychiatric patients and is related with unfavorable social background, worse global functioning, hostile or violent behavior, substance-related disorders. It appears also to be a significant risk of suicidal behavior.
- Published
- 2006
- Full Text
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48. Acridine Orange based platinum(II) complexes inducing cytotoxicity and cell cycle perturbation in spite of GSTP1 up-regulation.
- Author
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Valentini A, Pucci D, Crispini A, Federici G, and Bernardini S
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- Cell Line, Tumor, Humans, Acridine Orange chemistry, Acridine Orange toxicity, Cell Cycle drug effects, Glutathione S-Transferase pi genetics, Platinum chemistry, Platinum toxicity, Up-Regulation drug effects
- Abstract
A series of new ionic Pt(II) complexes of general formula [Pt(II)(A)n(Cl)(AO)]X (A=en, NH3; n=1, 2; X-=BF4-, NO3-, PF6-, CF3SO3-), 1-5, containing Acridine Orange (AO) bound to the metal atom through the endocyclic N atom, have been tested in human melanoma cells (M14, JR8 and PLF2), human neuroblastoma cell line SH-SY5Y and its cis-platin resistant subline SH-SY5Yres. The Pt(II) compounds, and in particular complexes 1 and 4, exhibit higher cytotoxic activity at lower concentration compared to cis-DDP in melanoma cells, affecting cell growth behavior and causing cell cycle perturbation. Moreover, M14 and JR8 cell lines were not able to rescue the impairment due to the new Pt(II) complexes since perturbation of cell cycle phases and cell proliferation inhibition were found after 72 h of recovery time. In order to evaluate whether GSTP1 may play a role in chemo-resistance of our melanoma model, we investigated the effect of the treatment with these Pt(II) compounds on GSTP1 gene expression. Up-regulation of GSTP1, evaluated by Qreal-time PCR was observed after treatment with complexes 1 and 4, showing that the effect of these Pt(II) compounds is GSTP1 indipendent. The lack of resistance of the new Pt(II)-AO complexes and their cytotoxicity, cell growth and cell cycle recovery in melanoma cells provide the basis for the development of new platinum anticancer compounds, directed to those tumors that over express GSTs enzymes.
- Published
- 2006
- Full Text
- View/download PDF
49. ATP-citrate lyase kinase and cyclic AMP-dependent protein kinase phosphorylate different sites on ATP-citrate lyase.
- Author
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Ramakrishna S, Pucci DL, and Benjamin WB
- Subjects
- Adipose Tissue enzymology, Animals, Cyclic AMP pharmacology, Liver enzymology, Muscles enzymology, Peptide Fragments analysis, Phosphorylation, Protein Binding, Rabbits, Rats, ATP Citrate (pro-S)-Lyase metabolism, Protein Kinases metabolism
- Abstract
ATP-citrate lyase was phosphorylated by highly purified cyclic AMP-independent protein kinase (ATP-citrate lyase kinase) or the catalytic subunit of cyclic AMP-dependent protein kinase. Each kinase phosphorylated ATP-citrate lyase equally but the combination of both kinases increased ATP-citrate lyase phosphorylation additively. When ATP-citrate lyase was phosphorylated with each kinase and partially digested with either L-1-tosylamido-2-phenylmethyl chloromethyl ketone-treated trypsin or Staphylococcus aureus protease followed by electrophoresis of the proteolytic products on sodium dodecyl sulfate-polyacrylamide gels or when the phosphorylated lyase was completely digested by these proteases followed by chromatography and electrophoresis, the results showed that the site phosphorylated by ATP-citrate lyase kinase was different from that phosphorylated by the catalytic subunit of cyclic AMP-dependent protein kinase. Only phosphoserine was found in lyase phosphorylated by the catalytic subunit of cyclic AMP-dependent protein kinase whereas phosphoserine and phosphothreonine were found in ATP-citrate lyase phosphorylated by lyase kinase.
- Published
- 1981
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