1. Optimization of the antimicrobial effect of blue light on methicillin-resistantStaphylococcus aureus(MRSA)in vitro
- Author
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Chukuka S. Enwemeka, Violet V. Bumah, Daniela S. Masson-Meyers, and Susan E. Cashin
- Subjects
Microbiological culture ,Strain (chemistry) ,Dermatology ,Bacterial growth ,Biology ,medicine.disease_cause ,biology.organism_classification ,Methicillin-resistant Staphylococcus aureus ,In vitro ,Microbiology ,Staphylococcus aureus ,medicine ,Surgery ,Irradiation ,Bacteria - Abstract
Background and Objective In previous studies, we showed that irradiation with 405 nm or 470 nm light suppresses up to 92% methicillin-resistant Staphylococcus aureus (MRSA) growth in vitro and that the remaining bacteria re-colonize. In this study, the aim was to develop a protocol that yields 100% MRSA growth suppression. Materials and Methods We cultured 3 × 106 and 5 × 106 CFU/ml USA300 strain of MRSA and then irradiated each plate with varying fluences of 1–60 J/cm2 of 405 nm or 470 nm light, either once or twice at 6 hours intervals. Next, we plated 7 × 106 CFU/ml and irradiated it with 45, 50, 55, or 60 J/cm2 fluence, once, twice, or thrice at the same 6 hours intervals. In a third experiment, the same culture density was irradiated with 0, 165, 180, 220, or 240 J/cm2, either once, twice, or thrice. Results Irradiation with either wavelength significantly reduced the bacterial colonies regardless of bacterial density (P
- Published
- 2015
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