1. CRA(Crosolic Acid) isolated from Actinidia valvata Dunn.Radix induces apoptosis of human gastric cancer cell line BGC823 in vitro via down-regulation of the NF-κB pathway
- Author
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Xiaohua Guo, Qi-Lai Cheng, Zhao-Wen Liu, Yi-Jian Cheng, Hongliang Li, and Ying-Chen Li
- Subjects
0301 basic medicine ,genetic structures ,Actinidia ,Population ,Down-Regulation ,Apoptosis ,Biology ,Toxicology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Stomach Neoplasms ,Cell Line, Tumor ,Survivin ,Humans ,education ,Cell Proliferation ,education.field_of_study ,Plant Extracts ,NF-kappa B ,NF-κB ,General Medicine ,Molecular biology ,Triterpenes ,In vitro ,IκBα ,030104 developmental biology ,chemistry ,Biochemistry ,Cell culture ,030220 oncology & carcinogenesis ,Corosolic acid ,Signal Transduction ,Food Science - Abstract
A natural ursolic compound, 2α,3β-dihydroxy-urs-12-en-28-oic acid (corosolic acid, CRA) was isolated from the root of Actinidia valvata Dunn. (A. valvata Radix). Since a large number of triterpenoid compound has marked anticancer effects toward various types of cancer cell lines in vitro, this study was carried out to investigate the anticancer effect of CRA in human gastric cancer cell line BGC823 cells and the underlying apoptotic mechanism of CRA was examined in BGC823 cell lines. The results showed that CRA significantly suppressed the viability of BGC823 cells in a concentration- and time-dependent manner. CRA also significantly increased the sub G1 population by cell cycle analysis in a concentration dependent manner. Exposure to CRA decreased p65, bcl-2, Fas, smac mRNA and protein expression, and increased IκBα, bax, survivin mRNA and protein expression. Results of immunofluorescence staining and EMSA further indicated CRA induced apoptosis by inhibiting nuclear translocation of nuclear factor NF-κB subunit p65. Consistently overall, our findings suggest that CRA induces apoptosis via inhibition of NF-κB (p65) expression level and activation of IκBα in BGC cells as a potent anticancer candidate for gastric cancer treatment.
- Published
- 2017