1. LIPUS inhibits inflammation and catabolism through the NF‐κB pathway in human degenerative nucleus pulposus cells.
- Author
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Yi, Weiwei, Chen, Qing, Liu, Chuan, Li, Kaiting, Tao, Bailong, Tian, Guihua, Zhou, Lu, Li, Xiaohong, Shen, Jieliang, Liu, Bo, Hu, Zhenming, Wang, Dawu, and Bai, Dingqun
- Subjects
INTERLEUKINS ,REVERSE transcriptase polymerase chain reaction ,CYTOKINES ,WESTERN immunoblotting ,INFLAMMATION ,METABOLISM ,GENE expression ,MATRIX metalloproteinases ,DNA-binding proteins ,TUMOR necrosis factors ,FLUORESCENT antibody technique ,DISCITIS ,CELL lines ,POLYMERASE chain reaction ,LUMBAR vertebrae ,ULTRASONICS - Abstract
Background: Low-intensity pulsed ultrasound (LIPUS) is a safe and noninvasive rehabilitative physical therapy with anti-inflammatory effects. The current study investigated the effect of LIPUS on the inflammation of nucleus pulposus (NP) cells and its underlying mechanism. Methods: Human NP cells were acquired from lumbar disc herniation tissue samples and cultured for experiments. Human NP cells were treated with LPS and then exposed to LIPUS (15 mW/cm
2 , 30 mW/cm2 and 60 mW/cm2 ) for 20 min daily for 3 days to determine the appropriate intensity to inhibit the expression of the inflammatory factors TNF-α and IL-1β. The gene and protein expression of aggrecan, collagen II, MMP-3 and MMP-9 was measured by real‐time PCR and western blotting, respectively. The activity of the nuclear factor‐kappa B (NF‐κB) pathway was examined by western blotting and immunofluorescence. After pretreatment with the NF-κB inhibitor PDTC, the expression of TNF-α, IL-1β, MMP-3 and MMP-9 was measured by real‐time PCR. Results: LIPUS at intensities of 15 mW/cm2 , 30 mW/cm2 and 60 mW/cm2 inhibited LPS-induced NP cell expression of the inflammatory factors TNF-α and IL-1β, especially at 30 mW/cm2 . LIPUS significantly upregulated the gene and protein expression of aggrecan and collagen II and downregulated the gene and protein expression of MMP-3 and MMP-9 in LPS-induced NP cells. The NF‐κB signaling pathway was inhibited by LIPUS through inhibiting the protein expression of p-P65 and the translocation of P65 into the nucleus in LPS-induced NP cells. In addition, LIPUS had similar effects as the NF-κB inhibitor PDTC by inhibiting the NF-κB signaling pathway, inflammation and catabolism in LPS-induced human degenerative nucleus pulposus cells. Conclusion: LIPUS inhibited inflammation and catabolism through the NF‐κB pathway in human degenerative nucleus pulposus cells. [ABSTRACT FROM AUTHOR]- Published
- 2021
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