76 results on '"Guo, Sheng"'
Search Results
2. CDK6 is a novel predictive and prognosis biomarker correlated with immune infiltrates in multiple human neoplasms, including small cell lung carcinoma
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Li, Guo-Sheng, Huang, Zhi-Guang, Li, Dong-Ming, Tang, Yu-Lu, Zheng, Jin-Hua, Yang, Lin, Feng, Yue, Peng, Jun-Xi, Li, Jing-Xiao, Tang, Yu-Xing, Zeng, Neng-Yong, Jin, Mei-Hua, Tian, Jia, Liu, Jun, Zhou, Hua-Fu, Chen, Gang, and Chen, Feng
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- 2023
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3. MMP12 serves as an immune cell–related marker of disease status and prognosis in lung squamous cell carcinoma
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Wei Zhang, Guo-Sheng Li, Xiang-Yu Gan, Zhi-Guang Huang, Rong-Quan He, Hong Huang, Dong-Ming Li, Yu-Lu Tang, Deng Tang, Wen Zou, Jun Liu, Yi-Wu Dang, Gang Chen, Hua-Fu Zhou, Jin-Liang Kong, and Hui-ping Lu
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Lung squamous cell carcinoma ,MMP12 ,Gene expression ,Prognosis ,Clinical value ,Immunity ,Medicine ,Biology (General) ,QH301-705.5 - Abstract
Background Worldwide, lung squamous cell carcinoma (LUSC) has wreaked havoc on humanity. Matrix metallopeptidase 12 (MMP12) plays an essential role in a variety of cancers. This study aimed to reveal the expression, clinical significance, and potential molecular mechanisms of MMP12 in LUSC. Methods There were 2,738 messenger RNA (mRNA) samples from several multicenter databases used to detect MMP12 expression in LUSC, and 125 tissue samples were validated by immunohistochemistry (IHC) experiments. Receiver operator characteristic (ROC) curves, Kaplan–Meier curves, and univariate and multivariate Cox regression analyses were used to assess the clinical value of MMP12 in LUSC. The potential molecular mechanisms of MMP12 were explored by gene enrichment analysis and immune correlation analysis. Furthermore, single-cell sequencing was used to determine the distribution of MMP12 in multiple tumor microenvironment cells. Results MMP12 was significantly overexpressed at the mRNA level (p
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- 2023
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4. SYNJ2 is a novel and potential biomarker for the prediction and treatment of cancers: from lung squamous cell carcinoma to pan-cancer
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Wei Hou, Guo-Sheng Li, Li Gao, Hui-Ping Lu, Hua-Fu Zhou, Jin-Liang Kong, Gang Chen, Shuang Xia, and Hong-Yu Wei
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Gene expression ,Prognosis ,Prediction ,Treatment ,Biomarker ,Immune ,Internal medicine ,RC31-1245 ,Genetics ,QH426-470 - Abstract
Abstract Background The roles and clinical values of synaptojanin 2 (SYNJ2) in lung squamous cell carcinoma (LUSC) remain unclear. Methods A total of 2824 samples from multi-center were collected to identify the expression of SYNJ2 in LUSC by using Wilcoxon rank-sum test, t-test, and standardized mean difference (SMD), and 194 in-house samples were also included to validate SYNJ2 expression in LUSC. The clinical roles of SYNJ2 were investigated via receiver operating characteristic (ROC) curves, univariate Cox regression analysis, and Kaplan–Meier plots. The underlying mechanisms of SYNJ2 in LUSC were explored by gene set enrichment analysis and immune correlation analysis. Further, a pan-cancer analysis based on 10,238 sapiens was performed to promote the understating of the expression and clinical significance of SYNJ2 in multiple human cancers. Results SYNJ2 was found to be significantly upregulated in LUSC at both mRNA and protein levels (p
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- 2022
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5. SYNJ2 is a novel and potential biomarker for the prediction and treatment of cancers: from lung squamous cell carcinoma to pan-cancer
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Hou, Wei, Li, Guo-Sheng, Gao, Li, Lu, Hui-Ping, Zhou, Hua-Fu, Kong, Jin-Liang, Chen, Gang, Xia, Shuang, and Wei, Hong-Yu
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- 2022
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6. Prognostic signature of esophageal adenocarcinoma based on pyroptosis-related genes
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Li, Guo-Sheng, He, Rong-Quan, Liu, Jun, He, Juan, Fu, Zong-Wang, Yang, Lin-Jie, Ma, Jie, Yang, Li-Hua, Zhou, Hua-Fu, Zeng, Jiang-Hui, and Chen, Gang
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- 2022
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7. Prognostic signature of esophageal adenocarcinoma based on pyroptosis-related genes
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Guo-Sheng Li, Rong-Quan He, Jun Liu, Juan He, Zong-Wang Fu, Lin-Jie Yang, Jie Ma, Li-Hua Yang, Hua-Fu Zhou, Jiang-Hui Zeng, and Gang Chen
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Adenocarcinoma of esophagus ,Pyroptosis ,Prognosis ,Gene expression ,Internal medicine ,RC31-1245 ,Genetics ,QH426-470 - Abstract
Abstract Background The role of pyroptosis-related genes (PRGs) in esophageal adenocarcinoma (EAC) remains unknown. Methods In this study, the first PRGs prognostic signature (PPS) of EAC was constructed based on the results of multivariate stepwise Cox regression analysis. Based on 1,047 samples of EAC and normal esophagus (NE), differentially expressed PRGs were selected for the establishment of the PPS. The discrimination effect of this PPS was detected by receiver operating characteristic curves, and the prognosis value of this PPS was determined through Cox regression analysis and Kaplan-Meier curves. Net benefits of the EAC patients from the nomogram (constructed based on the PPS and some clinical parameters) were assessed via decision curve analysis. The potential molecular mechanism of the PPS in EAC was explored via gene set enrichment analysis. The ability of PPS to distinguish EAC and NE was evaluated based on the results of summary receiver operating characteristic curves. Results The significant prognostic value of PPS can be observed at all of the training cohort, test cohort, and validation cohort, such as its independent risk role in the prognosis of the EAC patients (hazard ratio > 0; 95% CI not including 0). The positive net benefits of the nomogram for the EAC patients can be detected via decision curve analysis, and the potential molecular mechanism of the PPS in EAC is likely related to cell pyroptosis. Last, some of the PRGs (particularly CASP5) included in this PPS specifically support its feasibility for identifying EAC (area under the curves > 0.7). Conclusions The construction of this PPS in EAC enhances the present understanding of the relationship between PRGs and EAC, thus representing a novel approach to the clinical identification and management of EAC based on PRGs.
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- 2022
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8. Combination of transcriptional biomarkers and clinical parameters for early prediction of sepsis indued acute respiratory distress syndrome
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Ren-Qi Yao, Zong Shen, Qi-Min Ma, Ping Ling, Chen-Ru Wei, Li-Yu Zheng, Yu Duan, Wei Li, Feng Zhu, Yu Sun, and Guo-Sheng Wu
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sepsis ,acute respiratory distress syndrome ,prediction ,biomarker ,gene expression ,Immunologic diseases. Allergy ,RC581-607 - Abstract
ObjectiveAs a common yet intractable complication of severe sepsis, acute respiratory distress syndrome (ARDS) is closely associated with poor clinical outcomes and elevated medical expenses. The aim of the current study is to generate a model combining transcriptional biomarkers and clinical parameters to alarm the development of ARDS in septic patients.MethodsGene expression profile (GSE66890) was downloaded from the Gene Expression Omnibus database and clinical data were extracted. Differentially expressed genes (DEGs) from whole blood leukocytes were identified between patients with sepsis alone and septic patients who develop ARDS. ARDS prediction model was constructed using backward stepwise regression and Akaike Information Criterion (AIC). Meanwhile, a nomogram based on this model was established, with subsequent internal validation.ResultsA total of 57 severe septic patients were enrolled in this study, and 28 (49.1%) developed ARDS. Based on the differential expression analysis, six DEGs (BPI, OLFM4, LCN2, CD24, MMP8 and MME) were screened. According to the outcome prediction model, six valuable risk factors (direct lung injury, shock, tumor, BPI, MME and MMP8) were incorporated into a nomogram, which was used to predict the onset of ARDS in septic patients. The calibration curves of the nomogram showed good consistency between the probabilities and observed values. The decision curve analysis also revealed the potential clinical usefulness of the nomogram. The area under the receiver operating characteristic (AUROC) for the prediction of ARDS occurrence in septic patients by the nomogram was 0.86 (95% CI = 0.767-0.952). A sensitivity analysis showed that the AUROC for the prediction of ARDS development in septic patients without direct lung injury was 0.967 (95% CI = 0.896-1.0).ConclusionsThe nomogram based on transcriptional biomarkers and clinical parameters showed a good performance for the prediction of ARDS occurrence in septic patients.
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- 2023
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9. Integrated mRNA-miRNA transcriptome profiling of blood immune responses potentially related to pulmonary fibrosis in forest musk deer.
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Wen-Hua Qi, Li-Fan Hu, Yu-Jiawei Gu, Xiao-Yan Zhang, Xue-Mei Jiang, Wu-Jiao Li, Jun-Sheng Qi, Guo-Sheng Xiao, and Hang Jie
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PULMONARY fibrosis ,GENE expression ,IMMUNE response ,T cell receptors ,TRANSCRIPTOMES - Abstract
Background: Forest musk deer (FMD, Moschus Berezovskii) is a critically endangered species world-widely, the death of which can be caused by pulmonary disease in the farm. Pulmonary fibrosis (PF) was a huge threat to the health and survival of captive FMD. MicroRNAs (miRNAs) and messenger RNAs (mRNAs) have been involved in the regulation of immune genes and disease development. However, the regulatory profiles of mRNAs and miRNAs involved in immune regulation of FMD are unclear. Methods: In this study, mRNA-seq and miRNA-seq in blood were performed to constructed coexpression regulatory networks between PF and healthy groups of FMD. The hub immune- and apoptosis-related genes in the PF blood of FMD were explored through Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. Further, protein-protein interaction (PPI) network of immune-associated and apoptosis-associated key signaling pathways were constructed based on mRNA-miRNA in the PF blood of the FMD. Immune hub DEGs and immune hub DEmiRNAs were selected for experimental verification using RT-qPCR. Results: A total of 2744 differentially expressed genes (DEGs) and 356 differentially expressed miRNAs (DEmiRNAs) were identified in the PF blood group compared to the healthy blood group. Among them, 42 DEmiRNAs were negatively correlated with 20 immune DEGs from a total of 57 correlations. The DEGs were significantly associated with pathways related to CD molecules, immune disease, immune system, cytokine receptors, T cell receptor signaling pathway, Th1 and Th2 cell differentiation, cytokine-cytokine receptor interaction, intestinal immune network for IgA production, and NODlike receptor signaling pathway. There were 240 immune-related DEGs, in which 186 immune-related DEGs were up-regulated and 54 immune-related DEGs were down-regulated. In the protein-protein interaction (PPI) analysis of immune-related signaling pathway, TYK2, TLR2, TLR4, IL18, CSF1, CXCL13, LCK, ITGB2, PIK3CB, HCK, CD40, CD86, CCL3, CCR7, IL2RA, TLR3, and IL4R were identified as the hub immune genes. The mRNA-miRNA coregulation analysis showed that let-7d, miR-324-3p, miR-760, miR-185, miR-149, miR-149-5p, and miR-1842-5p are key miRNAs that target DEGs involved in immune disease, immune system and immunoregulation. Conclusion: The development and occurrence of PF were significantly influenced by the immune-related and apoptosis-related genes present in PF blood. mRNAs and miRNAs associated with the development and occurrence of PF in the FMD. [ABSTRACT FROM AUTHOR]
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- 2024
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10. Genome-wide identification and expression analysis of the KNOX family and its diverse roles in response to growth and abiotic tolerance in sweet potato and its two diploid relatives.
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Jia, Li-Cong, Yang, Zi-Tong, Shang, Li-Li, He, Shao-Zhen, Zhang, Huan, Li, Xu, and Xin, Guo-Sheng
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GENE expression ,SWEET potatoes ,HOMEOBOX genes ,GENOMICS ,HOMEOBOX proteins ,GENE families - Abstract
KNOXs, a type of homeobox genes that encode atypical homeobox proteins, play an essential role in the regulation of growth and development, hormonal response, and abiotic stress in plants. However, the KNOX gene family has not been explored in sweet potato. In this study, through sequence alignment, genomic structure analysis, and phylogenetic characterization, 17, 12 and 11 KNOXs in sweet potato (I. batatas, 2n = 6x = 90) and its two diploid relatives I. trifida (2n = 2x = 30) and I. triloba (2n = 2x = 30) were identified. The protein physicochemical properties, chromosome localization, phylogenetic relationships, gene structure, protein interaction network, cis-elements of promoters, tissue-specific expression and expression patterns under hormone treatment and abiotic stresses of these 40 KNOX genes were systematically studied. IbKNOX4, -5, and − 6 were highly expressed in the leaves of the high-yield varieties Longshu9 and Xushu18. IbKNOX3 and IbKNOX8 in Class I were upregulated in initial storage roots compared to fibrous roots. IbKNOXs in Class M were specifically expressed in the stem tip and hardly expressed in other tissues. Moreover, IbKNOX2 and − 6, and their homologous genes were induced by PEG/mannitol and NaCl treatments. The results showed that KNOXs were involved in regulating growth and development, hormone crosstalk and abiotic stress responses between sweet potato and its two diploid relatives. This study provides a comparison of these KNOX genes in sweet potato and its two diploid relatives and a theoretical basis for functional studies. [ABSTRACT FROM AUTHOR]
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- 2024
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11. An NGS-based assay for accurate detection and quantification of immune gene expression in mouse tumor models.
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Xue, Jia, Chen, Xiaobo, An, Xiaoyu, Wang, Jingjing, Zang, Mingfa, Mao, Binchen, Guo, Sheng, Yang, Tao, Kumari, Rajendra, and Li, Qi-Xiang
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GENE expression ,LABORATORY mice ,TUMOR microenvironment ,BIOMARKERS ,DYNAMICAL systems ,PROTEIN expression - Abstract
Tumor microenvironment (TME) is a complex dynamic system with many tumor-interacting components including tumor-infiltrating leukocytes (TILs), cancer associated fibroblasts, blood vessels, and other stromal constituents. It intrinsically affects tumor development and pharmacology of oncology therapeutics, particularly immune-oncology (IO) treatments. Accurate measurement of TME is therefore of great importance for understanding the tumor immunity, identifying IO treatment mechanisms, developing predictive biomarkers, and ultimately, improving the treatment of cancer. Here, we introduce a mouse-IO NGS-based (NGSmIO) assay for accurately detecting and quantifying the mRNA expression of 1080 TME related genes in mouse tumor models. The NGSmIO panel was shown to be superior to the commonly used microarray approach by hosting 300 more relevant genes to better characterize various lineage of immune cells, exhibits improved mRNA and protein expression correlation to flow cytometry, shows stronger correlation with mRNA expression than RNAseq with 10x higher sequencing depth, and demonstrates higher sensitivity in measuring low-expressed genes. We describe two studies; firstly, detecting the pharmacodynamic change of interferon-γ expression levels upon anti-PD-1: anti-CD4 combination treatment in MC38 and Hepa 1–6 tumors; and secondly, benchmarking baseline TILs in 14 syngeneic tumors using transcript level expression of lineage specific genes, which demonstrate effective and robust applications of the NGSmIO panel. [ABSTRACT FROM AUTHOR]
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- 2024
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12. Identifying cellular senescence associated genes involved in the progression of end-stage renal disease as new biomarkers.
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Xi, Yu-jia, Guo, Qiang, Zhang, Ran, Duan, Guo-sheng, and Zhang, Sheng-xiao
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GENE ontology ,CHRONIC kidney failure ,CELLULAR aging ,GENE expression ,BIOMARKERS ,GENES - Abstract
Background: Cellular senescence plays an essential role in the development and progression of end-stage renal disease (ESRD). However, the detailed mechanisms phenomenon remains unclear. Methods: The mRNA expression profiling dataset GSE37171 was taken from the Gene Expression Omnibus (GEO) database. The cell senescence-associated hub genes were selected by applying protein–protein interaction (PPI), followed by correlation analysis, gene interaction analysis, Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. We next explored the relationships of hub genes with miRNAs, TFs, and diseases. The absolute abundance of eight immune cells and two stromal cells were calculated by MCPcount and the correlation of hub genes with these ten cells was analyzed. Lasso was used to selecting for trait genes. ROC curves and DCA decision curves were used to assess the accuracy and predictive power of the trait genes. Results: A total of 65 cellular senescence signature genes were identified among patients and controls. The PPI network screened out ten hub genes. GO and KEGG indicated that ten hub genes were associated with ESRD progression. Transcription factor gene interactions and common regulatory networks of miRNAs were also identified in the datasets. The hub genes were significantly correlated with immune cells and stromal cells. Then the lasso model was constructed to screen out the five most relevant signature genes (FOS, FOXO3, SIRT1, TP53, SMARCA4). The area under the ROC curve (AUC) showed that these five characteristic genes have good resolving power for the diagnostic model. Conclusions: Our findings suggested that cellular senescence-associated genes played an important role in the development of ESRD and immune regulation. [ABSTRACT FROM AUTHOR]
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- 2023
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13. Icariin promotes the proliferation and osteogenic differentiation of bone-derived mesenchymal stem cells in patients with osteoporosis and T2DM by upregulating GLI-1.
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Xia, Sheng-li, Ma, Zi-yuan, Wang, Bin, Gao, Feng, Guo, Sheng-yang, and Chen, Xu-han
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BIOMARKERS ,FLAVONOIDS ,BONE growth ,HERBAL medicine ,SEQUENCE analysis ,RNA ,OSTEOPOROSIS ,TYPE 2 diabetes ,TREATMENT effectiveness ,BIOINFORMATICS ,GENE expression ,CELL proliferation ,RESEARCH funding ,POLYMERASE chain reaction ,MESENCHYMAL stem cells ,CHINESE medicine ,THERAPEUTICS - Abstract
Background: The function of mesenchymal stem cells (MSCs) from patients with osteoporosis (OP) is impaired and worsens in patients with type 2 diabetes mellitus (T2DM). Icariin (ICA) is the major active flavonoid glucoside isolated from traditional Chinese herbal Epimedium pubescens, and confirmed able to improve bone mass of OP patients. Objective: To investigate the effect of ICA on the proliferation and osteogenic differentiation of bone-derived MSCs (BMSCs) from patients with OP and T2DM and uncover the potential mechanism. Methods: BMSCs were treated with ICA, and proliferation and osteogenic potency were evaluated using the 2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and detection of osteogenic markers (ALP, RUNX2, SPP1, COL1A1, and mineralized nodules) was performed. RNA sequencing and bioinformatic analysis were performed to identify differentially expressed genes (DEGs) after ICA treatment and screen proliferation- and osteogenic differentiation-related processes. Gene gain and loss were performed to confirm the role of the key candidate gene. Results: ICA significantly promoted the proliferation and osteogenic differentiation of BMSCs. A total of 173 DEGs were identified after ICA treatment. Six DEGs (GLI-1, IGF2, BMP6, WNT5A, PTHLH, and MAPK14) enriched in both proliferation- and osteogenic differentiation-related processes were screened; GLI-1 had the highest validated |log2FC| value. Overexpression of GLI-1 enhanced the proliferation and osteogenic differentiation of BMSCs, and knockdown of GLI-1 weakened the positive effect of ICA on BMSCs. Conclusion: ICA promoted the proliferation and osteogenic differentiation of impaired BMSCs by upregulating GLI-1. [ABSTRACT FROM AUTHOR]
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- 2023
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14. CircPVT1 promotes ER‐positive breast tumorigenesis and drug resistance by targeting ESR1 and MAVS.
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Yi, Jia, Wang, Lei, Hu, Guo‐sheng, Zhang, Yue‐ying, Du, Jiao, Ding, Jian‐cheng, Ji, Xiang, Shen, Hai‐feng, Huang, Hai‐hua, Ye, Feng, and Liu, Wen
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BREAST ,CANCER cell growth ,DRUG resistance ,BRCA genes ,TYPE I interferons ,GENE expression - Abstract
The molecular mechanisms underlying estrogen receptor (ER)‐positive breast carcinogenesis and endocrine therapy resistance remain incompletely understood. Here, we report that circPVT1, a circular RNA generated from the lncRNA PVT1, is highly expressed in ERα‐positive breast cancer cell lines and tumor samples and is functionally important in promoting ERα‐positive breast tumorigenesis and endocrine therapy resistance. CircPVT1 acts as a competing endogenous RNA (ceRNA) to sponge miR‐181a‐2‐3p, promoting the expression of ESR1 and downstream ERα‐target genes and breast cancer cell growth. Furthermore, circPVT1 directly interacts with MAVS protein to disrupt the RIGI–MAVS complex formation, inhibiting type I interferon (IFN) signaling pathway and anti‐tumor immunity. Anti‐sense oligonucleotide (ASO)‐targeting circPVT1 inhibits ERα‐positive breast cancer cell and tumor growth, re‐sensitizing tamoxifen‐resistant ERα‐positive breast cancer cells to tamoxifen treatment. Taken together, our data demonstrated that circPVT1 can work through both ceRNA and protein scaffolding mechanisms to promote cancer. Thus, circPVT1 may serve as a diagnostic biomarker and therapeutic target for ERα‐positive breast cancer in the clinic. Synopsis: Estrogen receptor (ER)α, encoded by ESR1, promotes mammary malignancies and is targeted in cancer therapy; however, resistance arises frequently. This study reports a novel circRNA, termed circPVT1, with dual mechanisms to enhance ER‐positive breast tumorigenesis, suggesting therapeutic opportunities.CircPVT1 is highly expressed in ERα‐positive breast tumor patients.CircPVT1 sponges miR‐181a‐2‐3p to stabilize ESR1 mRNA, activating the expression of estrogen/ERα‐target genes.CircPVT1 interacts with MAVS protein to disrupt RIGI–MAVS complex formation, repressing the expression of type I IFN and interferon‐stimulated genes.ASO‐targeting circPVT1 suppresses ERα‐positive breast cancer cell and tumor growth. [ABSTRACT FROM AUTHOR]
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- 2023
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15. CEP55: an immune-related predictive and prognostic molecular biomarker for multiple cancers.
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Li, Guo-Sheng, Zhang, Wei, Huang, Wan-Ying, He, Rong-Quan, Huang, Zhi-Guang, Gan, Xiang-Yu, Yang, Zhen, Dang, Yi-Wu, Kong, Jin-Liang, Zhou, Hua-Fu, and Chen, Gang
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CRISPRS ,GENE expression ,BIOMARKERS - Abstract
Background: Centrosomal protein 55 (CEP55) plays a significant role in specific cancers. However, comprehensive research on CEP55 is lacking in pan-cancer. Methods: In-house and multi-center samples (n = 15,823) were used to analyze CEP55 in 33 cancers. The variance of CEP55 expression levels among tumor and control groups was evaluated by the Wilcoxon rank-sum test and standardized mean difference (SMD). The clinical value of CEP55 in cancers was assessed using receiver operating characteristic (ROC) curves, Cox regression analysis, and Kaplan-Meier curves. The correlations between CEP55 expression and the immune microenvironment were explored using Spearman's correlation coefficient. Results: The data of clustered regularly interspaced short palindromic repeats confirmed that CEP55 was essential for the survival of cancer cells in multiple cancer types. Elevated CEP55 mRNA expression was observed in 20 cancers, including glioblastoma multiforme (p < 0.05). CEP55 mRNA expression made it feasible to distinguish 21 cancer types between cancer specimens and their control samples (AUC = 0.97), indicating the potential of CEP55 for predicting cancer status. Overexpression of CEP55 was correlated with the prognosis of cancer individuals for 18 cancer types, exhibiting its prognostic value. CEP55 expression was relevant to tumor mutation burden, microsatellite instability, neoantigen counts, and the immune microenvironment in various cancers (p < 0.05). The expression level and clinical relevance of CEP55 in cancers were verified in lung squamous cell carcinoma using in-house and multi-center samples (SMD = 4.07; AUC > 0.95; p < 0.05). Conclusion: CEP55 may be an immune-related predictive and prognostic marker for multiple cancers, including lung squamous cell carcinoma. [ABSTRACT FROM AUTHOR]
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- 2023
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16. The difference of cadmium accumulation between the indica and japonica subspecies and the mechanism of it
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Zhou, Quan, Shao, Guo-Sheng, Zhang, Ying-Xing, Dong, Qing, Wang, Hong, Cheng, Shi-Hua, Cao, Li-Yong, and Shen, Xi-Hong
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- 2017
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17. Transcriptome analysis and identification of genes associated with floral transition and fruit development in rabbiteye blueberry (Vaccinium ashei)
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Jia-Xin Xiao, Lida Wang, Bo Zhu, Hong Zhang, Xuan Gao, and Guo-Sheng Lv
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Blueberry Plants ,Gene Expression ,Plant Science ,Biochemistry ,Anthocyanins ,chemistry.chemical_compound ,Gene Expression Regulation, Plant ,Plant Hormones ,chemistry.chemical_classification ,Multidisciplinary ,biology ,Plant Biochemistry ,Bud ,Plant Anatomy ,Jasmonic acid ,Eukaryota ,food and beverages ,Berries ,Vernalization ,Plants ,Blueberries ,Horticulture ,Medicine ,Buds ,Florigen ,Research Article ,Signal Transduction ,Vaccinium ,Science ,Flowers ,Plant disease resistance ,Fruits ,Auxin ,DNA-binding proteins ,Genetics ,Gene Regulation ,Gibberellic acid ,Gene Expression Profiling ,fungi ,Organisms ,Biology and Life Sciences ,Proteins ,biology.organism_classification ,Hormones ,Regulatory Proteins ,chemistry ,Fruit ,Auxins ,Transcriptome ,Transcription Factors - Abstract
Flowering and fruit set are important traits affecting fruit quality and yield in rabbiteye blueberry (Vaccinium ashei). Intense efforts have been made to elucidate the influence of vernalization and phytohormones on flowering, but the molecular mechanisms of flowering and fruit set remain unclear. To unravel these mechanisms, we performed transcriptome analysis to explore blueberry transcripts from flowering to early fruit stage. We divided flowering and fruit set into flower bud (S2), initial flower (S3), bloom flower (S4), pad fruit (S5), and cup fruit (S6) based on phenotype and identified 1,344, 69, 658, and 189 unique differentially expressed genes (DEGs) in comparisons of S3/S2, S4/S3, S5/S4, and S6/S5, respectively. There were obviously more DEGs in S3/S2 and S5/S4 than in S4/S3, and S6/S5, suggesting that S3/S2 and S5/S4 represent major transitions from buds to fruit in blueberry. GO and KEGG enrichment analysis indicated these DEGs were mostly enriched in phytohormone biosynthesis and signaling, transporter proteins, photosynthesis, anthocyanins biosynthesis, disease resistance protein and transcription factor categories, in addition, transcript levels of phytohormones and transporters changed greatly throughout the flowering and fruit set process. Gibberellic acid and jasmonic acid mainly acted on the early stage of flowering development like expression of the florigen gene FT, while the expression of auxin response factor genes increased almost throughout the process from bud to fruit development. Transporter proteins were mainly associated with minerals during the early flowering development stage and sugars during the early fruit stage. At the early fruit stage, anthocyanins started to accumulate, and the fruit was susceptible to diseases such as fungal infection. Expression of the transcription factor MYB86 was up-regulated during initial fruit development, which may promote anthocyanin accumulation. These results will aid future studies exploring the molecular mechanism underlying flowering and fruit set of rabbiteye blueberry.
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- 2021
18. The expression of tumstatin is down-regulated in renal carcinoma
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Xu, Chun-xiao, Liu, Xian-xi, Hou, Guo-sheng, Yan, Yun-fei, Chen, Shi-min, Wang, Wei, Jiang, Guang-shui, Liu, Bin, and Xin, Jia-xuan
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- 2010
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19. The reversal effect of Ginsenoside Rh2 on drug resistance in human colorectal carcinoma cells and its mechanism
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Gui-wei Liu, Yan-hua Liu, Guo-sheng Jiang, and Wei-dan Ren
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0301 basic medicine ,Cancer Research ,ATP Binding Cassette Transporter, Subfamily B ,Ginsenosides ,Cell ,Gene Expression ,Flow cytometry ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Cell Movement ,Tumor Cells, Cultured ,medicine ,Humans ,Cytotoxicity ,Cell Proliferation ,medicine.diagnostic_test ,Chemistry ,Carcinoma ,Cell Cycle ,Drug Synergism ,Cell migration ,Cell Biology ,Cell cycle ,030104 developmental biology ,medicine.anatomical_structure ,Drug Resistance, Neoplasm ,Ginsenoside ,Apoptosis ,030220 oncology & carcinogenesis ,Cancer research ,Fluorouracil ,Multidrug Resistance-Associated Proteins ,Stem cell ,Colorectal Neoplasms ,Phytotherapy - Abstract
Recent studies hint that Ginsenoside is involved in cancer prevention and treatment. In this study, we investigated the effect of Ginsenoside Rh2 on drug resistance in human colorectal carcinoma (CRC) cells and its mechanism. The resistance reversion effect of Ginsenoside Rh2 in CRC cells was analyzed using CCK-8 assay. After treating with Ginsenoside Rh2, the cell cycle distribution and cellular apoptosis were analyzed by flow cytometry, cell migration was determined by transwell migration assay, the expression of drug-resistance genes and proteins were evaluated using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot, respectively. Ginsenoside Rh2 could enhance the cytotoxicity of 5-FU in drug-resistant CRC cells (LoVo/5-FU and HCT-8/5-FU). Treatment with Ginsenoside Rh2 could result in an increase of cell numbers in G0/G1 phase accompanied with a decrease in S-phase, and induced cellular apoptosis in drug-resistant CRC cells. In addition, the migration process and EMT process of drug-resistant CRC cells were suppressed by treatment of Ginsenoside Rh2. Compared to control group, expression of drug-resistance genes, such as MRP1, MDR1, LRP and GST, were negatively correlated to Ginsenoside Rh2. All these results indicated that Ginsenoside Rh2 could effectively reverse drug resistance in human colorectal carcinoma cell and its mechanism involved the prevention of cellular proliferation and migration, the promotion of cellular apoptosis and the alteration of drug-resistance genes, which suggested that Ginsenoside Rh2 may act as a promising candidate for drug resistance in human colorectal carcinoma chemotherapy.
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- 2018
20. Expression profile of PU.1 in CD4+T cells from patients with systemic lupus erythematosus.
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Xiang, Nan, Fang, Xuan, Sun, Xiao-Ge, Zhou, Ying-Bo, Ma, Yan, Zhu, Chen, Li, Xiang-Pei, Wang, Guo-sheng, Tao, Jin-hui, and Li, Xiao-Mei
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SYSTEMIC lupus erythematosus ,MONONUCLEAR leukocytes ,GENE expression ,ENZYME-linked immunosorbent assay - Abstract
Systemic lupus erythematosus (SLE) is a multisystem autoimmune disease with complex genetic predisposing factors involved. PU.1 is an important member of the ETS transcription factors family which has diverse functions such as regulating the proliferation, differentiation of immune cells and multiple inflammatory cytokines. Previous studies preliminary explored the relation between PU.1 and SLE. To further explain the potential role of PU.1 in the pathogenesis of SLE, 40 SLE patients and 20 age-sex matched healthy controls (HC) were recruited in this study. Flow cytometry was used to test the percentages of CD4
+ PU.1+ T cells in peripheral blood mononuclear cells (PBMCs) from patients with SLE and HC. Expression levels of PU.1 mRNA in CD4+ T cells from SLE patients and HC were analyzed by real-time transcription-polymerase chain reaction. Expression levels of plasma IL-1β, IL-9, IL-18, IL-6, IFN-α, TNF-α, IL-10 and TGF-β1 were measured by enzyme-linked immunosorbent assay. The percentage of CD4+ PU.1+ T cells in PBMCs from patients with SLE was significantly higher than that from HC (P < 0.001). In addition, the PU.1 mRNA expression in CD4+ T cells from SLE patients was increased than that from HC (P = 0.002). In SLE patients, no significant correlation was found between the percentage of CD4+ PU.1+ T cells and the expression of PU.1 mRNA in CD4+ T cells (P > 0.05). Associations of PU.1 mRNA expression in CD4+ T cells with major clinical and laboratory parameters of SLE patients were also analyzed, but no significant correlations were found. Consistent with previous studies, SLE patients had increased IL-1β, IL-18, IL-6, IFN-α, TNF-α and IL-10 plasma concentrations than HC (P < 0.01). The expression level of plasma TGF-β1 was significantly decreased in SLE patients than in HC (P < 0.001). In SLE patients, the expression level of IL-1β was positive correlated with PU.1 mRNA expression in CD4+ T cells (P = 0.001). Our study first time evaluated the expression profile of PU.1 in CD4+ T cells from SLE patients confirming that PU.1 may participate in the pathogenesis of SLE. [ABSTRACT FROM AUTHOR]- Published
- 2021
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21. Comparative study of carvedilol and quinidine for inhibiting hKv4.3 channel stably expressed in HEK 293 cells
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Gui-Rong Li, Wei-Yin Wu, Zhi-Quan Wang, Guo-Sheng Xiao, Yi-Gang Li, Yan Wang, Ling-Jun Jie, Rui Zhang, and Hai-Ying Sun
- Subjects
0301 basic medicine ,Quinidine ,Male ,Heart Ventricles ,Action Potentials ,Gene Expression ,Pharmacology ,03 medical and health sciences ,Inhibitory Concentration 50 ,0302 clinical medicine ,medicine ,Potassium Channel Blockers ,Myocyte ,Animals ,Humans ,In patient ,Myocytes, Cardiac ,IC50 ,Carvedilol ,Brugada syndrome ,Chemistry ,Protein Stability ,HEK 293 cells ,Peak current ,medicine.disease ,Kinetics ,030104 developmental biology ,HEK293 Cells ,Shal Potassium Channels ,Rabbits ,Ion Channel Gating ,030217 neurology & neurosurgery ,medicine.drug - Abstract
The inhibition of transient outward potassium current (Ito) is the major ionic mechanism for quinidine to treat Brugada syndrome; however, quinidine is inaccessible in many countries. The present study compared the inhibitory effect of the nonselective β-adrenergic blocker carvedilol with quinidine on human Kv4.3 (hKv4.3, encoding for Ito) channel and action potential notch using a whole-cell patch technique in HEK 293 cell line expressing K C N D 3 as well as in ventricular epicardial myocytes of rabbit hearts. It was found that carvedilol and quinidine inhibited hKv4.3 current in a concentration-dependent manner. The IC50 of carvedilol was 1.2 μM for inhibiting hKv4.3 charge area, while the IC50 of quinidine was 2.9 μM (0.2 Hz). Both carvedilol and quinidine showed typical o p e n channel blocking properties (i.e. decreasing the time to peak of activation and increasing the inactivation of hKv4.3), negatively shifted the V1/2 of activation and inactivation, and slowed the recovery from inactivation of the channel. Although carvedilol had weaker in use- and rate-dependent inhibition of hKv4.3 peak current than quinidine, its reduction of the charge area was more than quinidine at all frequencies (0.2–3.3 Hz). Moreover, the inhibitory effect of carvedilol on action potential notch was greater than quinidine. These results provide the novel information that carvedilol, like quinidine, significantly inhibits hKv4.3 and action potential notch, suggesting that carvedilol is likely an alternative drug for preventing malignant ventricular arrhythmias in patients with Brugada syndrome in countries where quinidine is unavailable.
- Published
- 2018
22. Treatment with Lingqi capsules suppresses colorectal cancer by inhibiting the hepatocyte growth factor/c-Met signal transduction pathway
- Author
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Guo‑Sheng Wang, Jin‑Long Zhao, and Huan Li
- Subjects
Cancer Research ,medicine.medical_specialty ,C-Met ,Gene Expression ,Capsules ,Biology ,Cell morphology ,Biochemistry ,Mice ,chemistry.chemical_compound ,Internal medicine ,Genetics ,medicine ,Animals ,RNA, Messenger ,Molecular Biology ,Cell Proliferation ,Oncogene ,Hepatocyte Growth Factor ,Cell growth ,Proto-Oncogene Proteins c-met ,Cell cycle ,Antineoplastic Agents, Phytogenic ,Xenograft Model Antitumor Assays ,Rats ,Tumor Burden ,Disease Models, Animal ,Endocrinology ,Oncology ,chemistry ,Apoptosis ,Cell culture ,Cancer research ,Molecular Medicine ,Hepatocyte growth factor ,Colorectal Neoplasms ,Drugs, Chinese Herbal ,Signal Transduction ,medicine.drug - Abstract
Lingqi capsules (LQCs) are commonly used in Chinese herbal medicine to support the immune system and inhibit tumor growth. The capsules are considered to have a direct effect on tumor cell proliferation and support tumor cell apoptosis. In the present study, the effects of LQC serum on the colorectal cancer cell line, LoVo, and on tumors induced by the cell line were investigated in nude mice. LQC serum was generated by feeding Wistar rats LQC and isolating the serum from blood samples obtained from the rats. The serum was then used to treat LoVo cells for 24, 48 and 72 h, after which the cell morphology and proliferation were assessed. In addition, nude mice were injected with 0.2 ml LoVo cells subcutaneously to produce tumors. After 24 h, xenografted nude mice were treated with 5.0, 2.5 or 1.25 g/kg/day LQC serum by gavage for 21 days and the tumor growth, morphology, apoptosis of tumor cells and expression profiles of hepatocyte growth factor (HGF) and its receptor, c‑Met, were investigated. Compared with the negative controls, inhibition of cell growth was clearly visible in the LoVo cells treated for 24, 48 and 72 h and this inhibition was enhanced as the exposure time and drug concentration increased. The growth of solid tumors induced by the transplantation of LoVo cells into nude mice was inhibited to differing degrees. Following LQC treatment, the apoptotic rates of the cells were increased, the protein and mRNA expression levels of HGF were downregulated and those of c‑Met were upregulated. These findings suggest that LQC treatment inhibits colorectal cancer by downregulating the HGF/c‑Met signal transduction pathway.
- Published
- 2014
23. The clinical significance of interleukin 24 and its potential molecular mechanism in laryngeal squamous cell carcinoma.
- Author
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Tang, Xiao-Zhun, Zhou, Xian-Guo, Zhang, Xiao-Guohui, Li, Guo-Sheng, Chen, Gang, Dang, Yi-Wu, Huang, Zhi-Guang, Li, Ming-Xuan, Liang, Yao, Yao, Yu-Xuan, Chen, Xiao-Yi, Rong, Min-Hua, and Huang, Su-Ning
- Subjects
SQUAMOUS cell carcinoma ,RECEIVER operating characteristic curves ,CELL anatomy ,GENE expression ,EXTRACELLULAR matrix - Abstract
Interleukin 24 (IL24) has been documented to be highly expressed in several cancers, but its role in laryngeal squamous cell carcinoma (LSCC) remains unclarified. In this study, to reveal the function and its clinical significance of IL24 in LSCC, multiple detecting methods were used comprehensively. IL24 protein expression was remarkably higher in LSCC (n = 49) than non-cancerous laryngeal controls (n = 26) as detected by in-house immunohistochemistry. Meanwhile, the IL24 mRNA expression was also evaluated based on high throughput data from Gene Expression Omnibus, The Cancer Genome Atlas, ArrayExpress and Oncomine databases. Consistently with the protein level, IL24 mRNA expression level was also predominantly upregulated in LSCC (n = 172) compared to non-cancerous laryngeal tissues (n = 81) with the standard mean difference (SMD) being 1.25 and the area under the curve (AUC) of the summary receiver operating characteristic (sROC) being 0.89 (95% CI = 0.86–0.92). Furthermore, the related genes of IL24 and the differentially expressed genes (DEGs) of LSCC were intersected and sent for Gene ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, and the protein-protein interaction (PPI) analyses. In the GO annotation, the top terms of biological process (BP), cellular component (CC) and molecular function (MF) were extracellular matrix organization, extracellular matrix, cytokine activity, respectively. The top pathway of KEGG was ECM-receptor interaction. The PPI networks indicated the top hub genes of IL24-related genes in LSCC were SERPINE1, TGFB1, MMP1, MMP3, CSF2, and ITGA5. In conclusion, upregulating expression of IL24 may enhance the occurrence of LSCC, which owns prospect diagnostic ability and therapeutic significance in LSCC. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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24. Regulations of berberine on gene expression of BMP4 transcriptional pathways to improve visceral white adipose tissues insulin resistance in type 2 diabetic hamsters
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Xu-Han Liu, Xin-Yu Li, Lan Huang, Ya-Li Liu, Guo-Sheng Li, and Zheng-Nan Gao
- Subjects
Male ,0301 basic medicine ,medicine.medical_specialty ,Berberine ,Adipose Tissue, White ,Hamster ,Adipose tissue ,Bone Morphogenetic Protein 4 ,White adipose tissue ,Intra-Abdominal Fat ,Biology ,03 medical and health sciences ,chemistry.chemical_compound ,Insulin resistance ,Adipose Tissue, Brown ,Cricetinae ,Internal medicine ,Brown adipose tissue ,Gene expression ,medicine ,Animals ,Humans ,Insulin ,Pharmacology (medical) ,General Pharmacology, Toxicology and Pharmaceutics ,PRDM16 ,Activating Transcription Factor 2 ,medicine.disease ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,Diabetes Mellitus, Type 2 ,Gene Expression Regulation ,Complementary and alternative medicine ,chemistry ,Female ,Insulin Resistance - Abstract
To study the effects of berberine on the gene mRNA expressions of BMP4 transcriptional pathways and brown/white adipose tissue conversion transcriptional pathways in visceral white adipose tissues(VWAT) in type 2 diabetic hamsters and explore the relevant mechanisms. The obese insulin-resistant hamster model were induced by using high-fat diet, and then the type 2 diabetic hamster model was created through injection with low-dose streptozotocin in the obese insulin-resistant hamster model. After the modeling, the hamsters were randomly divided into normal control, obese insulin-resistant, type 2 diabetic and berberine-treated diabetic groups. After the nine-week treatment, real-time quantitative PCR was used to measure the changes in gene mRNA expressions of VWAT BMP4 transcriptional pathways, brown/white adipose tissue conversion transcriptional pathways and their target genes in different groups. The results showed that the gene mRNA expressions of BMP4, BMPRⅡ, BMPRlA, Smad1, Smad5, Smad8, p38/MAPK, ATF2, PRDM16, C/EBPβ, PGC1α, PPARγ and brown adipose tissue-specific genes was decreased and that of Smad6, Smurf1 and white adipose tissue-specific genes was increased in VWAT of model hamsters. Treatment with berberine regulated BMP4 transcriptional pathways and brown adipose tissue transcriptional pathways and induced the gene mRNA expression of brown adipose tissue-specific genes in VWAT to develop browning gene phenotype of white adipose tissues, and then improved fat-induced insulin resistance. These findings indicated that BMP4 transcriptional pathways involved in the formation of fat-induced visceral white adipose tissues insulin resistance (FIVWATIR) and the browning molecular mechanism of white adipose tissues induced by berberine.
- Published
- 2016
25. The expression of tumstatin is down-regulated in renal carcinoma
- Author
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Guo-Sheng Hou, Yun-fei Yan, Chun-xiao Xu, Xian-Xi Liu, Jia-Xuan Xin, Wei Wang, Guang-shui Jiang, Bin Liu, and Shi-min Chen
- Subjects
Collagen Type IV ,Regulation of gene expression ,Tumstatin ,Antibodies, Neoplasm ,Angiogenesis ,Down-Regulation ,Kidney metabolism ,General Medicine ,Biology ,Kidney ,Autoantigens ,Molecular biology ,Kidney Neoplasms ,Angiogenesis inhibitor ,Gene Expression Regulation, Neoplastic ,Blot ,Type IV collagen ,Gene expression ,Genetics ,Cancer research ,Humans ,Cloning, Molecular ,Molecular Biology - Abstract
Tumstatin is the 28 kDa NC1 domain of the alpha3 chain of type IV collagen that inhibits pathological angiogenesis and suppresses endothelial cell proliferation and tumor growth. In the present paper, we expressed and purified recombinant human tumstatin protein and then prepared the anti-tumstatin polyclonal antibody. To investigate the expression of tumstatin in renal carcinoma, tumstatin protein was detected by western blotting using the prepared anti-tumstatin antibody and tumstatin mRNA levels were assayed by RT-PCR. The results showed that the expression of tumstatin gene was down-regulated in renal carcinoma tissues and cells. Our study suggests that as a novel endogenous angiogenesis inhibitor, tumstatin gene expression may be a marker for diagnosis, therapy and prognosis of renal carcinoma.
- Published
- 2009
26. [Effect of hepatocyte growth factor and transforming growth factor-β(1) on atrial fibroblasts fibrosis]
- Author
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Jian-cheng, Zhang, Jian-quan, Chen, Chun-xuan, Xu, Lin, Chen, Ya-zhou, Lin, and Guo-sheng, Wu
- Subjects
Adult ,Male ,Adolescent ,Hepatocyte Growth Factor ,Rheumatic Heart Disease ,Gene Expression ,Fibroblasts ,Middle Aged ,Fibrosis ,Actins ,Collagen Type I ,Transforming Growth Factor beta1 ,Young Adult ,Atrial Fibrillation ,Humans ,Female ,Heart Atria ,RNA, Messenger ,Cells, Cultured - Abstract
To investigate the effect of hepatocyte growth factor (HGF) and transforming growth factor-β(1) (TGFβ(1)) on the expression of α-smooth muscle actin (α-SMA) and collagen I in human atrial fibroblast in vitro, and to explore the possible molecular mechanism of atrial fibrosis in patients with atrial fibrillation (AF).Human atrial fibroblast, isolated from aseptic right atrial appendage tissues of 10 sinus rhythm (SR) and 10 chronic atrial fibrillation (CAF) patients, were cultured with HGF and TGFβ(1). mRNA expressions of collagen I and α-SMA were detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), the protein expression of α-SMA was determined by immunofluorescence and Western blot.(1) Compared with SR group, left atrium was significantly dilated in CAF group (t = 2.692, P0.05), the mRNA expression of collagen I and α-SMA of atrial fibroblasts were significantly upregulated (all P0.01), mRNA expression of collagen I was positively correlated with left atrial dimension (LAD) (r = 0.836, P = 0.014), AF duration (r = 0.739, P = 0.045) and α-SMA mRNA level (r = 0.886, P = 0.012). (2) Compared with SR group, the expression of α-SMA protein in CAF atrial fibroblasts were significantly increased (P0.01). (3) TGFβ(1) further stimulated while HGF significantly attenuated the expression of collagen I and α-SMA in CAF atrial fibroblasts (all P0.01).Increasing expression of collagen I and α-SMA in human atrial fibroblasts might promote atria remodeling leading to the development and sustaining of AF. HGF is involved in the negative regulation on the expression of α-SMA and collagen I.
- Published
- 2013
27. Effects of equol on multiple K+ channels stably expressed in HEK 293 cells.
- Author
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Deng, Xiu-Ling, Wang, Yan, and Xiao, Guo-Sheng
- Subjects
VOLTAGE-clamp techniques (Electrophysiology) ,CARDIOVASCULAR disease treatment ,POTASSIUM channels ,GENE expression ,ACTION potentials ,ANIMAL models in research - Abstract
The present study investigated the effects of equol on cardiovascular K
+ channel currents. The cardiovascular K+ channel currents were determined in HEK 293 cells stably expressing cloned differential cardiovascular K+ channels with conventional whole-cell patch voltage-clamp technique. We found that equol inhibited hKv1.5 (IC50 : 15.3 μM), hKv4.3 (IC50 : 29.2 μM and 11.9 μM for hKv4.3 peak current and charge area, respectively), IKs (IC50 : 24.7 μM) and IhERG (IC50 : 31.6 and 56.5 μM for IhERG.tail and IhERG.step , respectively), but not hKir2.1 current, in a concentration-dependent manner. Interestingly, equol increased BKCa current with an EC50 of 0.1 μM. It had no significant effect on guinea pig ventricular action potentials at concentrations of ≤3 μM. These results demonstrate that equol inhibits several cardiac K+ currents at relatively high concentrations, whereas it increases BKCa current at very low concentrations, suggesting that equol is a safe drug candidate for treating patients with cerebral vascular disorders. [ABSTRACT FROM AUTHOR]- Published
- 2017
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28. Lnc RNA- H19 Modulates Wnt/β-catenin Signaling by Targeting Dkk4 in Hindlimb Unloaded Rat.
- Author
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Li, Bing, Liu, Jun, Zhao, Jie, Ma, Jian‐xiong, Jia, Hao‐bo, Zhang, Yang, Xing, Guo‐sheng, and Ma, Xin‐long
- Subjects
OSTEOPOROSIS treatment ,NON-coding RNA ,WNT signal transduction ,GENE expression ,NUCLEOTIDE sequence - Abstract
Objective To investigate the biological functions of long noncoding RNA-H19 ( H19) in the pathogenesis of disuse osteoporosis ( DOP). Methods Fifty-four male Sprague Dawley ( SD) rats were randomly divided into three groups: baseline control ( BC, 6), age-matched control ( AC, 24), and hindlimb unloading ( HLU, 24). The rats in the BC group were sacrificed at the beginning of the experiment, while the AC and HLU rats were sacrificed at different times (7, 14, 21 and 28 days after HLU). The DOP model was verified by micro- CT scan, and quantitative real-time polymerase chain reaction (q RT-PCR) was used to quantify the expression of osteogenic genes ( OPG, Run X2 and OPG). Gene sequencing and bioinformatic analysis were performed to find H19 target genes and the associated signaling pathway, which were first verified on tissue samples. Further verification was performed by knocking down the H19 and related gene in rat osteoblast cell line ( UMR106 cell). Then, the changes of associated signaling pathway and osteogenic function were examined to confirm the prediction of the bioinformatic analysis. Results Micro- CT scans and quantitative real-time polymerase chain reaction (q RT-PCR) tests showed progressively deteriorated trabecular bone and decreased level of osteogenic genes in the metaphysis of distal femur during HLU, indicating the successful establishment of a DOP model. According to RNA sequencing, 1351 m RNA and 464 lnc RNA were abnormally expressed in response to mechanical unloading, in which the H19 decreased 2.86 fold in HLU rats. There were 1426 m RNA predicted to be the target genes of H19, and KEGG pathway analysis suggested that Wnt signaling pathway ( Wnt signaling) was the top pathway responsible for these target genes. In the Wnt-associated genes targeted by H19, 11 were differentially expressed between HLU and AC rats, among which Dkk4 increased 2.44 fold in HLU rats when compared to normal controls. These results of sequencing and bioinformatic analysis were confirmed by the low expression of H19, overexpression of Dkk4 and inhibited Wnt signaling observed in DOP rats. Subsequent in vitro cell assay further demonstrated that knockdown of H19 led to upregulation of Dkk4, and inhibition of Wnt signaling and osteogenic function in UMR106 cell. These effects can be greatly reversed after application of knocking down Dkk4. Conclusion Our findings demonstrated that low expression of H19, induced by mechanical unloading, leads to development of DOP through inhibition of Wnt signaling by promoting Dkk4 expression. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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29. Ultraviolet B exposure of peripheral blood mononuclear cells of patients with systemic lupus erythematosus inhibits DNA methylation
- Author
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Guo-Sheng Wang, Min Zhang, You Min Wang, M Kan, Xiang Pei Li, Hong Zhang, and Wen Chi Chen
- Subjects
Adult ,DNA (Cytosine-5-)-Methyltransferase 1 ,Male ,Exacerbation ,Adolescent ,Ultraviolet Rays ,DNA methyltransferase ,Peripheral blood mononuclear cell ,Pathogenesis ,Young Adult ,Rheumatology ,immune system diseases ,Gene expression ,Medicine ,Humans ,Lupus Erythematosus, Systemic ,DNA (Cytosine-5-)-Methyltransferases ,skin and connective tissue diseases ,Glucocorticoids ,Lupus erythematosus ,integumentary system ,business.industry ,DNA Methylation ,Middle Aged ,medicine.disease ,Immunology ,DNA methylation ,DNMT1 ,Cancer research ,Leukocytes, Mononuclear ,Female ,business - Abstract
Systemic lupus erythematosus (SLE) is an autoimmune inflammatory disease, in which sunlight (especially ultraviolet B (UVB) 290–320 nm) is known to induce exacerbation of disease. DNA methylation regulates gene expression, and hypomethylation is associated with abnormal cell function in SLE. The purpose of this study was to investigate the effect of UVB on DNA methylation in SLE and its significance in the pathogenesis of SLE. Forty-five patients with SLE and 20 healthy controls were enrolled in the study, which involved the investigation of DNA methylation and DNA methyltransferase 1 (DNMT1) of peripheral blood mononuclear cells with UVB irradiation. Our results demonstrate the following: The level of DNA methylation in patients with SLE was lower than that in the control group. DNA methylation was decreased after UVB irradiation at different dosages especially in patients with marlar rashes and leucopenia, but no significant difference was observed in the DNMT1 mRNA expression. DNA methylation levels in patients with active SLE were more sensitive to UVB. In conclusion, UVB exposure is able to inhibit DNA methylation, which subsequently takes part in the pathogenesis of SLE.
- Published
- 2009
30. Anti-inflammation role for mesenchymal stem cells transplantation in myocardial infarction
- Author
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Cuiyu Bao, Mingyan Hu, Guo-sheng Lin, Zhimin Hu, and Jun Guo
- Subjects
Male ,medicine.medical_specialty ,Immunology ,Interleukin-1beta ,Myocardial Infarction ,Gene Expression ,Inflammation ,Mesenchymal Stem Cell Transplantation ,Collagen Type I ,Muscle hypertrophy ,Rats, Sprague-Dawley ,Left coronary artery ,Internal medicine ,medicine.artery ,medicine ,Immunology and Allergy ,Animals ,Myocardial infarction ,Ventricular remodeling ,Tissue Inhibitor of Metalloproteinase-1 ,business.industry ,Interleukin-6 ,Tumor Necrosis Factor-alpha ,Myocardium ,Mesenchymal stem cell ,Mesenchymal Stem Cells ,medicine.disease ,Rats ,Transplantation ,Preload ,Myocarditis ,Collagen Type III ,Echocardiography ,Cardiology ,Hypertrophy, Left Ventricular ,medicine.symptom ,Matrix Metalloproteinase 1 ,business - Abstract
The aim of the present study was to investigate the role of anti-inflammation for MSCs transplantation in rat models of myocardial infarction. Rats with AMI induced by occlusion of the left coronary artery were randomized to MSCs transplantation group, MI group and sham operated group. The effects of MSCs transplantation on cardiac inflammation and left ventricular remodeling in non-infarcted zone were observed after 4 weeks of MI. We found that MSC transplantation (1) decreased protein production and gene expression of inflammation cytokines TNF-alpha, IL-1beta and IL-6, (2) inhibited deposition of type I and III collagen, as well as gene and protein expression of MMP-1 and TIMP-1, (3) attenuated LV cavitary dilation and transmural infarct thinning, thus prevent myocardial remodeling after myocardial infarction, and (4) increased EF, FS, LVESP and dp/dtmax (P < 0.01), decreased LVDd, LVEDV, LVEDP (P < 0.05). Anti-inflammation role for MSCs transplantation might partly account for the cardiac protective effect in ischemic heart disease.
- Published
- 2007
31. Chromic-P32 phosphate treatment of implanted pancreatic carcinoma: Mechanism involved
- Author
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Guan-Sheng Tong, Lu Liu, Yu Wang, Cheng Li, Guo-Sheng Feng, Wen Gao, Hong Gao, and Ying Huang
- Subjects
Male ,Pathology ,medicine.medical_specialty ,Cellular differentiation ,Tumor Cell Necrosis ,Mice, Nude ,Antineoplastic Agents ,Apoptosis ,Biology ,Flow cytometry ,Phosphates ,Mice ,Microscopy, Electron, Transmission ,Chromium Compounds ,Gene expression ,medicine ,Tumor Cells, Cultured ,Animals ,Humans ,Cell Nucleus ,Mice, Inbred BALB C ,medicine.diagnostic_test ,Gastroenterology ,Cell Differentiation ,General Medicine ,Proliferating cell nuclear antigen ,body regions ,Pancreatic Neoplasms ,Cell nucleus ,Lymphatic system ,medicine.anatomical_structure ,Basic Research ,biology.protein ,Neoplasm Transplantation - Abstract
AIM: To study the effects of chromic-P32 phosphate (32P colloids) interstitial administration in Pc-3 implanted pancreatic carcinoma, and investigate its anticancer mechanism. METHODS: Ninety-eight tumor bearing nude mice were killed at different time points after the injection of 32P colloids to the tumor core with observed radioactivity. The light microscopy, transmission electron microscopy (TEM) and immuno-histochemistry and flow cytometry were used to study the rates of tumor cell necrosis, proliferating cell nuclear antigen index, the micro vessel density (MVD). The changes of the biological response to the lymphatic transported 32P colloids in the inguinal lymph node (ILN) were dynamically observed, and the percentage of tumor cell apoptosis, and Apo2.7, caspase-3, Bcl-2, Bax-related gene expression were observed too. RESULTS: The half-life of effective medication is 13 d after injection of 32P colloids to the tumor stroma, in 1-6 groups, the tumor cell necrosis rates were 20%, 45%, 65%, 70%, 95% and 4%, respectively (F = 4.14-105.36, P
- Published
- 2005
32. Correlation of Surface Toll-Like Receptor 9 Expression with IL-17 Production in Neutrophils during Septic Peritonitis in Mice Induced by E. coli.
- Author
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Ren, Yunjia, Hua, Li, Meng, Xiuping, Xiao, Yue, Hao, Xu, Guo, Sheng, Zhao, Peiyan, Wang, Luowei, Dong, Boqi, Yu, Yongli, and Wang, Liying
- Subjects
PERITONITIS ,TOLL-like receptors ,INTERLEUKIN-17 ,GENE expression ,NEUTROPHILS ,LABORATORY mice ,DIAGNOSIS - Abstract
IL-17 is a proinflammatory cytokine produced by various immune cells. Polymorphonuclear neutrophils (PMNs) are the first line of defense in bacterial infection and express surface Toll-like receptor 9 (sTLR9). To study the relationship of sTLR9 and IL-17 in PMNs during bacterial infection, we infected mice with E. coli intraperitoneally to establish a septic peritonitis model for studying the PMNs response in peritoneal cavity. We found that PMNs and some of “giant cells” were massively accumulated in the peritoneal cavity of mice with fatal septic peritonitis induced by E. coli. Kinetically, the CD11b
+ PMNs were increased from 20–40% at 18 hours to >80% at 72 hours after infection. After E. coli infection, sTLR9 expression on CD11b+ and CD11b− PMNs and macrophages in the PLCs were increased at early stage and deceased at late stage; IL-17 expression was also increased in CD11b+ PMNs, CD11b− PMNs, macrophages, and CD3+ T cells. Using experiments of in vitro blockage, qRT-PCR and cell sorting, we confirmed that PMNs in the PLCs did increase their IL-17 expression during E. coli infection. Interestingly, sTLR9− CD11b+ Ly6G+ PMNs, not sTLR9+ CD11b+ Ly6G+ PMNs, were found to be able to increase their IL-17 expression. Together, the data may help understand novel roles of PMNs in septic peritonitis. [ABSTRACT FROM AUTHOR]- Published
- 2016
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33. HBV inhibits apoB production via the suppression of MTP expression
- Author
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Xinghui Liu, Fu-Bing Wang, Cheng-liang Zhu, and Guo-sheng Gao
- Subjects
Hepatitis B virus ,Apolipoprotein B ,Endocrinology, Diabetes and Metabolism ,Clinical Biochemistry ,Down-Regulation ,Gene Expression ,microsomal triglyceride transfer protein ,medicine.disease_cause ,Microsomal triglyceride transfer protein ,Endocrinology ,Hepatitis B, Chronic ,Western blot ,Gene expression ,medicine ,apolipoprotein B ,Humans ,RNA, Messenger ,chronic HBV infection ,lipid metabolism ,lcsh:RC620-627 ,Apolipoproteins B ,Biochemistry, medical ,Messenger RNA ,medicine.diagnostic_test ,biology ,hepatitisB virus ,Research ,Biochemistry (medical) ,Transfection ,Hep G2 Cells ,Molecular biology ,digestive system diseases ,Metabolism disorder ,lcsh:Nutritional diseases. Deficiency diseases ,Case-Control Studies ,biology.protein ,lipids (amino acids, peptides, and proteins) ,Carrier Proteins - Abstract
Background Liver dominates the production and secretion of apolipoprotein B (apoB) and evidence shows that liver malfunction induced by hepatitis B virus (HBV) infection could lead to apolipoprotein metabolism disorders. The present study was undertaken to assess the effects of HBV on apoB expression. Methods Clinical examination: serum apoB levels in patients with chronic HBV infection and in healthy individuals were measured by immunoturbidimetry using biochemical analyzer Olympus 5400. Cell study: mRNA and protein expression levels of apoB in HepG2 and HepG2.2.15 cells were measured by RT-PCR and Western blot. Alternatively, HBV infectious clone pHBV1.3 or control plasmid pBlue-ks were tranfected into HepG2 cells, and mRNA and protein expression levels of apoB, as well as the microsomal triglyceride transfer protein (MTP) in tranfected HepG2 cells were also measured by RT-PCR and western blot. Results Serum apoB level was much lower in chronic HBV patients as compared to healthy individuals (P < 0.05). Expression of apoB mRNA and protein was lower in HepG2.2.15 cells than in HepG2 cells. Similarly, expression of apoB mRNA and protein was lower in pHBV1.3 transfected HepG2 cells than in pBlue-ks transfected HepG2 cells. Expression of MTP mRNA and protein in pHBV1.3 transfected HepG2 cells was reduced in a dose-dependent fashion. Conclusion HBV infection plays an inhibitory effect on apoB expression.
- Published
- 2011
34. Correlation between mutation of MDR3 gene exon 6 and parenteral nutrition-associated cholestasis of preterm infants.
- Author
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XIU FANG YANG, GUO SHENG LIU, and BING YI
- Subjects
- *
GENE expression , *MULTIDRUG resistance , *CHOLESTASIS , *LEUCOCYTES , *ALLELES - Abstract
The aim of this study was to investigate the association between the mutation of multidrug resistance 3 (MDR3) exon 6 and parenteral nutrition-associated cholestasis (PNAC) in preterm infants. A total of 41 preterm infants with PNAC formed the experimental group, and 56 preterm infants receiving total parenteral nutrition (TPN) for >14 days but without cholestasis formed the control group. Genomic DNA was extracted from peripheral venous blood leukocytes. Polymerase chain reaction was used to amplify exon 6 of the MDR3 gene. The target band of MDR3 gene exon 6 was identified in all blood samples from all cases. We identified five cases with C. 504 C>T heterozygous mutations of exon 6 of the MDR3 gene and 14 cases with C. 504 C>T homozygous mutations in the experimental group. In the control group, we identified seven cases with the C. 504 C>T homozygous mutation and six cases with the C. 504 C>T heterozygous mutation. The distribution of the T/C allele frequency of C. 504 in exon 6 of the MDR3 gene between the experimental group and control group was statistically significant (P<0.05). Further analysis revealed the odds ratio of the T/C allele frequency of the C. 504 mutation in exon 6 of the MDR3 gene between the experimental group and control group to be 0.316. Point mutation C. 485 T>A was detected in one case in the experimental group. The C. 504 C>T and C. 485 T>A MDR3 mutations in exon 6 are possibly responsible for the development of PNAC in infants. C. 504 C>T may not be the only risk factor of neonatal PNAC. In order to further confirm the association between exon 6 of the MDR3 gene and PNAC, a large-sample multicenter study should be carried out. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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35. Expression of Ets-1 and FOXP3 mRNA in CD4CD25 T regulatory cells from patients with systemic lupus erythematosus.
- Author
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Xiang, Nan, Li, Xiang-Pei, Li, Xiao-Mei, Wang, Guo-Sheng, Tao, Jin-Hui, Pan, Hai-Feng, Fang, Xuan, Ma, Qian, and Yu, Ning
- Subjects
GENE expression ,FORKHEAD transcription factors ,MESSENGER RNA ,CD4 antigen ,T cells ,SYSTEMIC lupus erythematosus ,AUTOIMMUNE diseases ,PATIENTS ,PHYSIOLOGY - Abstract
Systemic lupus erythematosus (SLE) is a multisystem autoimmune disease with complex genetic predisposing factors involved. Ets-1 transcription factor plays an important role in the suppressive activity of CD4CD25 Treg cells and stable expression of FOXP3. To find its potential role in the pathogenesis of SLE, we investigate the mRNA expression of Ets-1 and FOXP3 mRNA in CD4CD25 Treg cells from patients with SLE. Real-time transcription-polymerase chain reaction analysis was used to determine the expression of Ets-1 and FOXP3 mRNA in CD4CD25 Treg cells from 36 patients with SLE and 18 sex-and-age-matched healthy controls. The Ets-1 mRNA expression level was decreased in patients with SLE [0.225 (0.135, 0.337)] than healthy controls [0.528 (0.303, 0.681)] ( P < 0.001). The expression levels of FOXP3 mRNA were lower in SLE patients [0.608 (0.272, 1.164)] than healthy controls [0.919 (0.690, 1.223)], but the difference was not significant ( P = 0.106). Significant reduction in Ets-1 and FOXP3 expression was also found in new-onset SLE subgroup when compared with healthy controls ( P < 0.001). The level of Ets-1 and FOXP3 mRNA was not significantly different in hyperactive and lower active SLE group when compared with inactive SLE group, respectively ( P > 0.05). There were no significant differences between SLE with lupus nephritis (LN) and SLE without LN either ( P > 0.05). Associations of Ets-1 and FOXP3 mRNA expression levels with major clinical and laboratory parameters of SLE patients were also analyzed. However, no significant association was found. Significant positive correlation was found between Ets-1 and FOXP3 mRNA expression in CD4CD25 Treg cells from SLE patients ( r = 0.698, P < 0.001). Our results found that the expression levels of Ets-1 mRNA were decreased in SLE patients and Ets-1 expression was positively correlated with the expression of FOXP3. It indicated that Ets-1 may play an important role in the stable expression of FOXP3 in CD4CD25 Treg cells. [ABSTRACT FROM AUTHOR]
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- 2014
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36. Silencing of PCDH10 in hepatocellular carcinoma via de novo DNA methylation independent of HBV infection or HBX expression.
- Author
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Fang, Song, Huang, Shi-feng, Cao, Ju, Wen, Yang-an, Zhang, Li-Ping, and Ren, Guo-Sheng
- Subjects
LIVER cancer ,DNA methylation ,GENE silencing ,HEPATITIS B ,GENE expression ,TUMOR suppressor genes - Abstract
PCDH10 is a key tumor suppressive gene for nasopharyngeal, esophageal, and other carcinomas with frequent methylation. In this study, we investigated the potential epigenetic modification of the PCDH10 gene by hepatitis B virus × protein (HBx), a pivotal factor in the progression of HBV replication and potential carcinogenesis. PCDH10 expression was found to be down-regulated in 9/13 (69.2 %) of hepatocellular carcinoma (HCC) cell lines. Decreased PCDH10 expression was correlated with the methylation status of the PCDH10 promoter. Treatment with the DNA methyltransferase inhibitor 5-aza-2′-deoxycytidine (Aza) was sufficient to restore PCDH10 mRNA expression by suppressing PCDH10 promoter methylation in HepG2 cells. Treatment with Trichostatin A alone had no significant effect on PCDH10 expression but enhanced the effect of Aza. PCDH10 methylation was further detected in 76 % (38 of 50) of HCC tissues compared with 40 % (20 of 50) of paired adjacent tissues, with no methylation detected in normal human liver tissues. There were significant correlations between methylation status of PCDH10 and tumor size, serum AFP levels, metastasis or TNM staging ( P < 0.05). Moreover, PCDH10 promoter methylation status was not associated with HBV infection in our panel of 50 primary HCC tumors, and transfection with HBX could not alter the status of PCDH10 promoter methylation. Collectively, these observations suggested that the expression of PCDH10 was silenced in HCC via de novo DNA methylation independent of HBV infection or HBX expression, and PCDH10 might form a potentially useful therapeutic target for HCC. [ABSTRACT FROM AUTHOR]
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- 2013
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37. Downregulation of mi R-101 in gastric cancer correlates with cyclooxygenase-2 overexpression and tumor growth.
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He, Xiao-Pu, Shao, Yun, Li, Xiao-Lin, Xu, Wei, Chen, Guo-Sheng, Sun, Huan-Huan, Xu, Hai-Chen, Xu, Xian, Tang, Dan, Zheng, Xi-Feng, Xue, Yi-Ping, Huang, Guo-Chang, and Sun, Wei-Hao
- Subjects
STOMACH cancer ,CYCLOOXYGENASE 2 ,GENE expression ,TUMOR growth ,DISEASE progression ,GENETIC transcription ,NON-coding RNA - Abstract
Cyclooxygenase-2 ( COX-2) plays an important role in the carcinogenesis and progression of gastric cancer. It has been demonstrated that COX-2 overexpression depends on different cellular pathways, involving both transcriptional and post-transcriptional regulation. Micro RNAs (mi RNAs) are small, noncoding RNAs that function as post-transcriptional regulators. Here, we characterize miR-101 expression and its role in the regulation of COX-2 expression, which in turn, will provide us with additional insights into the potential therapeutic benefits of exogenous mi R-101 for treatment of gastric cancer. Our results showed that mi R-101 levels in gastric cancer tissues were significantly lower than those in the matched normal tissue ( P < 0.01). Furthermore, lower levels of mi R-101 were associated with increased tumor invasion and lymph node metastasis ( P < 0.05). We also found an inverse correlation between mi R-101 and COX-2 expression in both gastric cancer specimens and cell lines. Significant decreases in COX-2 mRNA and COX-2 levels were observed in the pre-mi R-101-infected gastric cancer cells. One possible mechanism of interaction is that mi R-101 inhibited COX-2 expression by directly binding to the 3′- UTR of COX-2 m RNA. Overexpression of mi R-101 in gastric cancer cell lines also inhibited cell proliferation and induced apoptosis in vitro, as well as inhibiting tumor growth in vivo. These results collectively indicate that mi R-101 may function as a tumor suppressor in gastric cancer, with COX-2 as a direct target. [ABSTRACT FROM AUTHOR]
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- 2012
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38. Down-regulation of Z39Ig on macrophages by IFN-γ in patients with chronic HBV infection
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Guo, Sheng, Yang, Chengying, Mei, Feng, Wu, Shengxi, Luo, Na, Fei, Lei, Chen, Yongwen, and Wu, Yuzhang
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CELLULAR control mechanisms , *MACROPHAGES , *HEPATITIS B virus , *IMMUNOHISTOCHEMISTRY , *INTERFERONS , *VIRUS diseases , *GENE expression , *CELL physiology , *PATIENTS - Abstract
Abstract: Co-inhibitory signals from the B7 superfamily have been demonstrated to induce T cell dysfunction in chronic HBV infection (CHB). However, the expression and function of Z39Ig, a new inhibitor of the B7 superfamily, is still unclear in CHB. Here immunohistochemical staining showed that Z39Ig was restricted to macrophages and that its level was decreased significantly in CHB patients compared to healthy controls. Moreover, reduced Z39Ig expression was positively correlated with plasma HBV load but was inversely related to serum alanine aminotransaminase levels. Further, Z39Ig mRNA had a negative relation to IFN-γ in vivo, and IFN-γ also down-regulated Z39Ig expression on monocyte-derived macrophages (MDMs) in a time- and dose-dependent manner in vitro. Interestingly, Z39Ig expression on MDMs was restored when IFN-γ neutralizing antibodies were added to the T cell/MDM co-culture system, indicating that the IFN-γ derived from activated-T cells may contribute to the reduction of Z39Ig in the CHB environment. Our results suggest that T cells can opposite T cell hyporesponsiveness through dampening Z39Ig inhibitory signals from macrophages and thus maintain their anti-viral function in CHB. Therefore, decreasing Z39Ig signals from macrophages could contribute to CHB clinical therapy. [Copyright &y& Elsevier]
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- 2010
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39. The expression of tumstatin is down-regulated in renal carcinoma.
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Chun-xiao Xu, Xian-xi Liu, Guo-sheng Hou, Yun-fei Yan, Shi-min Chen, Wei Wang, Guang-shui Jiang, Bin Liu, and Jia-xuan Xin
- Abstract
Tumstatin is the 28 kDa NC1 domain of the α3 chain of type IV collagen that inhibits pathological angiogenesis and suppresses endothelial cell proliferation and tumor growth. In the present paper, we expressed and purified recombinant human tumstatin protein and then prepared the anti-tumstatin polyclonal antibody. To investigate the expression of tumstatin in renal carcinoma, tumstatin protein was detected by western blotting using the prepared anti-tumstatin antibody and tumstatin mRNA levels were assayed by RT-PCR. The results showed that the expression of tumstatin gene was down-regulated in renal carcinoma tissues and cells. Our study suggests that as a novel endogenous angiogenesis inhibitor, tumstatin gene expression may be a marker for diagnosis, therapy and prognosis of renal carcinoma. [ABSTRACT FROM AUTHOR]
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- 2010
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40. Expression and purification of the recombinant Fasciola gigantica cathepsin L1 in Escherichla coli.
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Guo Min, He Guo-sheng, Xu Mei-qian, Gao Xin-chun, and Yue Cheng
- Abstract
The article discusses a study on the gene expression and screening of the recombinant Fasciola gigantica cathepsin L1 (FgCL1) in Eschericia coli using polymerase chain reaction (PCR).
- Published
- 2008
41. Anti-Inflammation Role for Mesenchymal Stem Cells Transplantation in Myocardial Infarction.
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Jun Guo, Guo-sheng Lin, Cui-yu Bao, Zhi-min Hu, and Ming-yan Hu
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INFLAMMATION , *STEM cell transplantation , *MYOCARDIAL infarction , *GENE expression - Abstract
Abstract  The aim of the present study was to investigate the role of anti-inflammation for MSCs transplantation in rat models of myocardial infarction. Rats with AMI induced by occlusion of the left coronary artery were randomized to MSCs transplantation group, MI group and sham operated group. The effects of MSCs transplantation on cardiac inflammation and left ventricular remodeling in non-infarcted zone were observed after 4 weeks of MI. We found that MSC transplantation (1) decreased protein production and gene expression of inflammation cytokines TNF-α, IL-1β and IL-6, (2) inhibited deposition of type I and III collagen, as well as gene and protein expression of MMP-1 and TIMP-1, (3) attenuated LV cavitary dilation and transmural infarct thinning, thus prevent myocardial remodeling after myocardial infarction, and (4) increased EF, FS, LVESP and dp/dtmax (PâPâ [ABSTRACT FROM AUTHOR]
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- 2007
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42. Transcriptomic characterization and innovative molecular classification of clear cell renal cell carcinoma in the Chinese population.
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Zhao, Qiang, Xue, Jia, Hong, Baoan, Qian, Wubin, Liu, Tiezhu, Fan, Bin, Cai, Jie, Ji, Yongpeng, Liu, Jia, Yang, Yong, Li, Qixiang, Guo, Sheng, and Zhang, Ning
- Subjects
CHINESE people ,RENAL cell carcinoma ,RENAL cancer ,GENE fusion ,GENE expression - Abstract
Background: Large-scale initiatives like The Cancer Genome Atlas (TCGA) performed genomics studies on predominantly Caucasian kidney cancer. In this study, we aimed to investigate genomics of Chinese clear cell renal cell carcinoma (ccRCC). Methods: We performed whole-transcriptomic sequencing on 55 tumor tissues and 11 matched normal tissues from Chinese ccRCC patients. We systematically analyzed the data from our cohort and comprehensively compared with the TCGA ccRCC cohort. Results: It found that PBRM1 mutates with a frequency of 11% in our cohort, much lower than that in TCGA Caucasians (33%). Besides, 31 gene fusions including 5 recurrent ones, that associated with apoptosis, tumor suppression and metastasis were identified. We classified our cohort into three classes by gene expression. Class 1 shows significantly elevated gene expression in the VEGF pathway, while Class 3 has comparably suppressed expression of this pathway. Class 2 is characterized by increased expression of extracellular matrix organization genes and is associated with high-grade tumors. Applying the classification to TCGA ccRCC patients revealed better distinction of tumor prognosis than reported classifications. Class 2 shows worst survival and Class 3 is a rare subtype ccRCC in the TCGA cohort. Furthermore, computational analysis on the immune microenvironment of ccRCC identified immune-active and tolerant tumors with significant increased macrophages and depleted CD4 positive T-cells, thus some patients may benefit from immunotherapies. Conclusion: In summary, results presented in this study shed light into distinct genomic expression profiles in Chinese population, modified the stratification patterns by new molecular classification, and gave practical guidelines on clinical treatment of ccRCC patients. [ABSTRACT FROM AUTHOR]
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- 2020
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43. Molecular characterization of a teleost-specific toll-like receptor 22 (tlr22) gene from yellow catfish (Pelteobagrus fulvidraco) and its transcriptional change in response to poly I:C and Aeromonas hydrophila stimuli.
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Wei, Xiu-Ying, Wang, Jun, Guo, Sheng-Tao, Lv, Yun-Yun, Li, Yan-Ping, Qin, Chuan-Jie, Zou, Yuan-Chao, Shi, Qing-Chao, Hu, Peng, Xiong, Xiao-Qin, He, Yang, Li, Rui, Huang, Ze-Jin, Chen, Dun-Xue, and Wen, Zheng-Yong
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FLATHEAD catfish , *TOLL-like receptors , *AEROMONAS hydrophila , *PATTERN perception receptors , *FISH feeds , *GENE expression , *AMINO acid sequence - Abstract
Toll-like receptors (TLRs) are a class of pattern recognition receptors (PRRs) that can recognize pathogen-associated molecular patterns (PMPs) and play important roles in the innate immune system in vertebrates. In this study, we identified a teleost-specific tlr22 gene from yellow catfish (Pelteobagrus fulvidraco) and its immune roles in response to different pathogens were also determined. The open reading frame (ORF) of the tlr22 was 2892 bp in length, encoding a protein of 963 amino acids. Multiple protein sequences alignment, secondary and three-dimensional structure analyses revealed that TLR22 is highly conserved among different fish species. Phylogenetic analysis showed that the phylogenetic topology was divided into six families of TLR1 , TLR3 , TLR4 , TLR5 , TLR7 and TLR11 , and TLR22 subfamily was clustered into TLR11 family. Meanwhile, synteny and gene structure comparisons revealed functional and evolutionary conservation of the tlr22 gene in teleosts. Furthermore, tlr22 gene was shown to be widely expressed in detected tissues except barbel and eye, with highest expression level in liver. The transcription of tlr22 was significantly increased in spleen, kidney, liver and gill tissues at different timepoints after Poly I:C infection, suggesting TLR22 plays critical roles in defensing virus invasion. Similarly, the transcription of tlr22 was also dramatically up-regulated in spleen, kidney and gill tissues with different patterns after Aeromonas hydrophila infection, indicating that TLR22 is also involved in resisting bacteria invasion. Our findings will provide a solid basis for the investigation the immune functions of tlr22 gene in teleosts, as well as provide useful information for disease control and treatment for yellow catfish. • A teleost-specific toll-like receptor 22 (tlr22) gene was systematically identified in yellow catfish (Pelteobagrus fulvidraco). • Genetic synteny, gene structure, and phylogenetic analyses revealed that tlr22 is highly conserved in teleost. • The distribution pattern of tlr22 was determined, with highest expression level in liver. • Functional experiments revealed TLR22 is involved in resisting both bacterium and virus infections. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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44. Network pharmacology analysis and experimental verification of the antithrombotic active compounds of trichosanthis pericarpium (Gualoupi) in treating coronary heart disease.
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Xia, Kai-rou, Zhang, Xiao-yu, Zhang, Huang-qin, Su, Ke-lei, Shang, Er-xin, Xiao, Qing-ling, Li, Wei-wen, Guo, Sheng, Duan, Jin-ao, and Liu, Pei
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CHINESE medicine , *BIOLOGICAL models , *IN vitro studies , *RUTIN , *ARGININE , *CORONARY disease , *MELONS , *PROTEIN kinases , *PHENOMENOLOGICAL biology , *PHARMACEUTICAL chemistry , *FIBRINOLYTIC agents , *CELLULAR signal transduction , *DIETARY fats , *DESCRIPTIVE statistics , *OXIDATIVE stress , *BIOCHEMISTRY , *PLANT extracts , *RATS , *PROTHROMBIN , *BLOOD coagulation factors , *GENE expression , *ANIMAL experimentation , *AMINO acids , *FIBRINOGEN , *ORGANIC compounds , *INFLAMMATION - Abstract
Trichosanthis pericarpium (TP; Gualoupi, pericarps of Trichosanthes kirilowii Maxim) has been used in traditional Chinese medicine (TCM) to reduce heat, resolve phlegm, promote Qi, and clear chest congestion. It is also an essential herbal ingredient in the "Gualou Xiebai" formula first recorded by Zhang Zhongjing (from the Eastern Han Dynasty) in the famous TCM classic "Jin-Guì-Yào-Lüe" for treating chest impediments. According to its traditional description, Gualou Xiebai is indicated for symptoms of chest impediments, which correspond to coronary heart diseases (CHD). This study aimed to identify the antithrombotic compounds in Gualoupi for the treatment of CHD. A CHD rat model was established with a combination of high-fat diet and isoproterenol hydrochloride (ISO) administration via subcutaneous multi-point injection in the back of the neck. This model was used to evaluate the antithrombotic effect of two mainstream cultivars of TP ("HaiShi GuaLou" and "WanLou") by analyzing the main components and their effects. Network pharmacology, molecular docking-based studies, and a zebrafish (Danio rerio) thrombosis model induced by phenylhydrazine was used to validate the antithrombosis components of TP. TP significantly reduced the body weight of the CHD rats, improved myocardial ischemia, and reduced collagen deposition and fibrosis around the infarcted tissue. It reduced thrombosis in a dose-dependent manner and significantly reduced inflammation and oxidative stress damage. Cynaroside, isoquercitrin, rutin, citrulline, and arginine were identified as candidate active TP compounds with antithrombotic effects. The key potential targets of TP in thrombosis treatment were initially identified by molecular docking-based analysis, which showed that the candidate active compounds have a strong binding affinity to the potential targets (protein kinase C alpha type [PKCα], protein kinase C beta type [PKCβ], von Willebrand factor [vWF], and prostaglandin-endoperoxide synthase 1 [PTGS1], fibrinogen alpha [Fga], fibrinogen beta [Fgb], fibrinogen gamma [Fgg], coagulation factor II [F2], and coagulation factor VII [F7]). In addition, the candidate active compounds reduced thrombosis, improved oxidative stress damage, and down-regulated the expression of thrombosis-related genes (PKCα, PKCβ, vWF, PTGS1, Fga, Fgb, Fgg, F2, and F7) in the zebrafish model. Cynaroside, isoquercitrin, rutin, citrulline, and arginine were identified as the active antithrombotic compounds of TP used to treat CHD. Mechanistically, the active compounds were found to be involved in oxidative stress injury, platelet activation pathway, and complement and coagulation cascade pathways. [Display omitted] • GUALOUPI (TP) exhibits superior antithrombotic effects in treating CHD. • Cynaroside, etc were the antithrombotic active components of TP in treating CHD. • Three pathways such as platelet activation were important regulatory pathways. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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45. Overexpression of DNMT1 leads to hypermethylation of H19 promoter and inhibition of Erk signaling pathway in disuse osteoporosis.
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Li, Bing, Zhao, Jie, Ma, Jian-xiong, Li, Guo-min, Zhang, Yang, Xing, Guo-sheng, Liu, Jun, and Ma, Xin-long
- Subjects
- *
OSTEOPOROSIS , *DNA methyltransferases , *GENETIC overexpression , *GENE expression , *JAK-STAT pathway - Abstract
Disuse osteoporosis (DOP) is a common complication of the lack of mechanical loading. The precise mechanism underlying DOP remains unknown, although epigenetic modifications may be a major cause. Recently, cumulative research has revealed that DNA methyltransferase (DNMT) proteins can catalyze the conversion of cytosine to 5-methylcytosine (5mC), altering the epigenetic state of DNA. Here, we report that DNMT1 expression and lncRNA-H19 methylation are upregulated in the femoral tissues of DOP rats, accompanied with inhibited Erk signaling pathway. Overexpression of DNMT1 in UMR-106 cells mimics 5mC enrichment in the H19 promoter, inhibition of Erk signaling and impairment of osteogenesis, which can be rescued by 5′-aza-deoxycytidine (5′-Aza) treatment. Moreover, local intramedullary injection of Dnmt1 siRNA (siDNMT1) in Sprague-Dawley (SD) rats abrogated disuse lncRNA-H19 (H19) downregulation, Erk signaling inhibition, histopathological changes, and bone microstructure declines in the distal femur in vivo . Therefore, our data identify for the first time a new signaling cascade in DOP: mechanical unloading causes upregulation of DNMT1 and hypermethylation of H19 promoter, which subsequently leads to downregulation of lncRNA-H19 and inhibition of the ERK signaling, suggesting a new potential therapeutic target. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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46. Renal protective effect and action mechanism of Huangkui capsule and its main five flavonoids.
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Cai, Hong-Die, Su, Shu-Lan, Qian, Da-Wei, Guo, Sheng, Tao, Wei-Wei, Cong, Xu Dong, Tang, Renmao, and Duan, Jin-Ao
- Subjects
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REACTIVE oxygen species , *ANIMAL experimentation , *BIOFLAVONOIDS , *CHRONIC kidney failure , *GENE expression , *CHINESE medicine , *RATS , *WESTERN immunoblotting ,THERAPEUTIC use of plant extracts - Abstract
Ethnopharmacological relevance The flower of Abelmoschus manihot (Linn.) Medicus ( A. manihot), as a traditional Chinese Herbal medicine, was used widely in China with efficacy of inducing diuresis for treating strangurtia, and subdhing swelling and detoxicating. It has been reported that Huangkui capsule, prepared by the extract of the flower of A. manihot , can reduce the content of urinary protein, serum creatinine and serum urea nitrogen in nephropathy rats and processes renoprotective activity, while the action mechanism need to illuminate deeply. Aims of the study In this study, we investigated the protection effect of Huangkui capsule on tubulointerstitial fibrosis in chronic renal failure (CRF) rats and its mechanism against high glucose-induced epithelial to mesenchymal transition (EMT) in renal tubular epithelial cells (HK-2) of its bioactive components. Materials and methods The animals were divided into normal group, CRF model group and Huangkui capsule-treated group. Hematoxylin eosin (HE) staining and Masson staining were applied to observe pathological changes in renal tissue of different groups. Biochemical indicators including serum urea nitrogen (BUN), urine protein (UP) and serum creatinine (Scr) were measured according to the manufacturer's instructions of kits. HK-2 cell damaged model was established to access the protection effect and action mechanism of five main flavonoids from Huangkui capsule. The experimental cells were divided into eight groups: control group, model group, positive drug group and five main flavonoids treated groups. The dichlorodihydrofluorescein diacetate (DCFH-DA) assay was used to determine the reactive oxygen species (ROS) in different groups. Western blot was applied to analyze the expression of pathogenesis-related proteins in different groups. Results The results stated that Huangkui capsule significantly inhibited the elevation of Scr, BUN, UP, the expression of α-smooth muscle actin (α-SMA), phosphorylation-extracellular signal-regulated kinase (p-ERK1/2), NADPH Oxidase 1, NADPH Oxidase 2 and NADPH Oxidase 4 in adenine-induced CRF rats. The main bioactive components of quercetin (QT), hyperoside (HY), isoquercitrin (IQT), gossypetin-8- O - β -D-glucuronide (GG) and quercetin-3′- O -glucoside (QG) at the dosage of 100 µM, like NADPH oxidase inhibitor diphenyleneiodonium, exhibited a significant effect on inhibiting the expression of α-SMA, p-ERK1/2, NADPH Oxidase 1, NADPH Oxidase 2 and NADPH Oxidase 4 in high glucose-induced HK-2 cells, especially GG. Conclusions These results demonstrated that Huangkui capsule and the flavonoids components prevent tubulointerstitial fibrosis in CRF rat involvement in the action mechanism of inhibiting NADPH oxidase/ROS/ERK pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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47. Cytokine changes in response to TPO receptor agonist treatment in primary immune thrombocytopenia.
- Author
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Qu, Ming-ming, Liu, Xue-na, Liu, Xin-guang, Feng, Qi, Liu, Yang, Zhang, Xu, Liu, Shuang, Zhang, Lei, Li, Guo-sheng, Zhu, Yuan-yuan, Lv, Ming-yun, Peng, Jun, and Hou, Ming
- Subjects
- *
IDIOPATHIC thrombocytopenic purpura , *CYTOKINES , *THROMBOPOIETIN receptors , *MESSENGER RNA , *GENE expression , *THERAPEUTICS - Abstract
Thrombopoietin receptor agonists (TPO-RAs) have been clinically used in primary immune thrombocytopenia (ITP) with favorable outcomes, while their effect on cytokine regulation in ITP remains unknown. In the present study, plasma and mRNA expression levels of interleukin (IL)-2, interferon gamma (IFN-γ), IL-4, IL-17A, and transforming growth factor-β1 (TGF-β1) were determined by ELISA and real-time quantitative PCR in 26 corticosteroid-resistant/relapsed ITP patients receiving eltrombopag or rhTPO therapy and 15 healthy controls (HCs). Results showed that plasma and mRNA levels of IL-2, IFN-γ, IL-4, and IL-17A in ITP patients did not change significantly after TPO-RA treatment, whereas TGF-β1 levels increased remarkably. The pre- and post-treatment plasma and mRNA levels of IFN-γ and IL-2 were significantly higher, while the pre- and post-treatment IL-4 levels as well as the pre-treatment TGF-β1 levels were remarkably lower in ITP patients compared with HCs. There was no significant difference in TGF-β1 levels between TPO-RA-treated ITP patients and HCs. No statistical difference was found in plasma levels of IL-17A between ITP patients before or after treatment and HCs. However, the pre- and post-treatment mRNA expression of IL-17A and retinoic orphan receptor (ROR) γt in ITP patients were higher than that in HCs. Overall, these findings indicated that TPO-RA treatment could promote the secretion of TGF-β1, while it could not correct the Th1 and Th17 polarization in ITP patients. This study might improve our understanding of the mechanism of action of TPO-RAs and provide important information for optimizing therapeutic strategies for ITP. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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48. Thrombopoietin receptor agonists shift the balance of Fcγ receptors toward inhibitory receptor IIb on monocytes in ITP.
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Xin-guang Liu, Shuang Liu, Qi Feng, Xue-na Liu, Guo-sheng Li, Zi Sheng, Peng Chen, Yang Liu, Yu Wei, Xiao-yuan Dong, Ping Qin, Chengjiang Gao, Chunhong Ma, Lei Zhang, Ming Hou, and Jun Peng
- Subjects
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THROMBOPOIETIN receptor agonists , *CYTOKINE receptors , *GENE expression , *MONONUCLEAR leukocytes , *HEMATOPOIETIC growth factors , *PHYSIOLOGY - Abstract
Elevated expression of the activating Fcγ receptor (FcγR) I and FcγRIIa together with decreased expression of the inhibitory FcγRIIb are involved in the pathogenesis of primary immune thrombocytopenia (ITP). Thrombopoietin receptor agonists (TPO-RAs) have been used clinically for the management of ITP; however, little is known about the effect of TPO-RAs on FcγR modulation in ITP. In this prospective study, we measured the alteration in monocyte FcγR expression from 21 corticosteroid-resistant/relapsed patients with chronic ITP receiving eltrombopag therapy. Results showed that the mRNA and protein levels of FcγRIIb were significantly elevated after 6-week eltrombopag treatment. Concurrently, FcγRI and IIa levels decreased remarkably, whereas FcγRIII expression did not change. In vitro phagocytosis assays indicated that a shift in the balance of FcγR toward inhibitory FcγRIIb on monocytes was accompanied with a considerable decrease in monocyte/macrophage phagocytic capacity. The response to eltrombopag therapy in patients with ITP was associated with FcγR phenotype and functional changes of monocytes/macrophages. Moreover, the plasma transforming growth factor-bβ (TGF-bβ) concentrations increased significantly in eltrombopag responders. Modulation of monocyte FcγR balance by TPO-RAs was also found in a murine model of ITP established by transferring splenocytes from immunized CD61 knockout mice into CD61+ severe combined immunodeficient mice. Romiplostim administration in ITP mice significantly upregulated inhibitory FcγRII expression and downregulated activating FcγRI expression. These findings showed that recovery of platelet counts after TPO-RA treatment in ITP is associated with the restoration of FcγR balance toward the inhibitory FcγRIIb on monocytes, and suggested that thrombopoietic agents have a profound effect on immune modulation in ITP. This study is registered at ClinicalTrials.gov as #NCT01864512. [ABSTRACT FROM AUTHOR]
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- 2016
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49. The function of aquaporin4 in ischemic brain edema.
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Wen-Wen Wang, Cheng-long Xie, Li-Li Zhou, and Guo-Sheng Wang
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AQUAPORIN genetics , *CEREBRAL ischemia , *CEREBRAL edema , *CAUSES of death , *INTRACRANIAL pressure , *BRAIN damage , *GENE expression - Abstract
Cerebral ischemia injury is a primary cause of human death and long-term disability. We know that the cerebral edema induced by ischemia injury has a fatal effect on humans, which is the main cause of death for cerebral ischemia because it produces elevated intracranial pressure that leads to secondary brain damage, such as further impaired vascular perfusion and herniation of brain. Therefore, reducing the severity of brain edema has become the main therapeutic strategy for the treatment of CI. However, current treatment options for brain edema are limited and problematic. Therefore, finding novel strategies for overcoming this problem is crucial. Numerous studies demonstrated that cerebral edema may be attenuated via the regulation of AQP4 expression, thus initiating a novel therapeutic strategy against this possibly fatal condition. This review focuses on the role of AQP4 in ischemic brain edema, and its prospect as a therapeutic target. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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50. Recombinant Hepatitis B Virus Surface Antigen Formulated with B-Type CpG Oligodeoxynucleotide Induces Therapeutic Immunity Against Hepatitis B Virus Surface Antigen-Expressing Liver Cancer Cells in Mice.
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Zhou, Xiaojing, Wei, Hongfei, Sun, Peng, Wu, Xiuli, Wan, Min, Zhang, Peng, Guo, Sheng, Zhao, Tiesuo, Yu, Yongli, and Wang, Liying
- Subjects
- *
RECOMBINANT viruses , *HEPATITIS B virus , *CELL surface antigens , *CPG nucleotides , *GENE expression , *LIVER cancer , *CANCER immunotherapy , *CANCER cells , *LABORATORY mice - Abstract
AbstractTo develop a therapeutic vaccine against hepatitis B virus surface antigen (HBsAg)-expressing liver cancer, we tried to prepare a vaccine by formulating recombinant HBsAg with BW006, a B type CpG oligodeoxynucleotide (ODN) with Th1-biasing activity, and examined its potency of inducing therapeutic immunity against HBsAg-expressing liver cancer cells in mice. When applied therapeutically, BW006 could assist HBsAg to induce vigorous immune responses capable of inhibiting the growth of HBsAg-expressing liver cancer cells and prolonging the survival of mice bearing HBsAg-expressing liver cancer cells. In vivoand in vitroexperiments showed that the BW006-adjuvanted HBsAg enhanced the production of IgG2a antibodies, interferon-γ, and interleukin-12 and facilitated the generation of specific cytotoxic T lymphocyte that killed the HBsAg-expressing liver cancer cells. These results suggest that the BW006-adjuvanted HBsAg might be developed into a candidate tumor vaccine for the treatment of HBsAg-expressing liver cancer. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
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