1. Identification and cloning of human mitochondrial translational release factor 1 and the ribosome recycling factor
- Author
-
Yuelin Zhang and Linda L. Spremulli
- Subjects
Ribosomal Proteins ,DNA, Complementary ,Saccharomyces cerevisiae Proteins ,Molecular Sequence Data ,Biophysics ,Ribosome Recycling Factor ,Biochemistry ,Ribosome ,Mitochondrial Proteins ,Complete sequence ,Structural Biology ,Complementary DNA ,Escherichia coli ,Genetics ,Mitochondrial ribosome ,Humans ,Coding region ,Amino Acid Sequence ,Cloning ,biology ,Proteins ,Peptide Chain Termination, Translational ,Molecular biology ,Mitochondria ,Cell biology ,biology.protein ,Release factor ,Sequence Alignment ,Transcription Factors - Abstract
The complete sequence of the cDNA for the coding region of human mitochondrial translational release factor 1 has been obtained from human EST clones and 5′RACE. This sequence has been analyzed to provide insights into the relationship between release factors from bacteria and from mammalian mitochondria. The complete cDNA for the human mitochondrial ribosome recycling factor has been assembled using EST clones. This factor has been expressed in Escherichia coli and purified as a His-tagged protein.
- Published
- 1998