1. Exploring the metabolic profile of A. baumannii for antimicrobial development using genome-scale modeling.
- Author
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Leonidou, Nantia, Xia, Yufan, Friedrich, Lea, Schütz, Monika S., and Dräger, Andreas
- Subjects
CARBAPENEM-resistant bacteria ,METABOLIC models ,BACTERIAL metabolism ,BIOMASS production ,HUMAN genes ,ACINETOBACTER baumannii - Abstract
With the emergence of multidrug-resistant bacteria, the World Health Organization published a catalog of microorganisms urgently needing new antibiotics, with the carbapenem-resistant Acinetobacter baumannii designated as "critical". Such isolates, frequently detected in healthcare settings, pose a global pandemic threat. One way to facilitate a systemic view of bacterial metabolism and allow the development of new therapeutics is to apply constraint-based modeling. Here, we developed a versatile workflow to build high-quality and simulation-ready genome-scale metabolic models. We applied our workflow to create a metabolic model for A. baumannii and validated its predictive capabilities using experimental nutrient utilization and gene essentiality data. Our analysis showed that our model iACB23LX could recapitulate cellular metabolic phenotypes observed during in vitro experiments, while positive biomass production rates were observed and experimentally validated in various growth media. We further defined a minimal set of compounds that increase A. baumannii's cellular biomass and identified putative essential genes with no human counterparts, offering new candidates for future antimicrobial development. Finally, we assembled and curated the first collection of metabolic reconstructions for distinct A. baumannii strains and analyzed their growth characteristics. The presented models are in a standardized and well-curated format, enhancing their usability for multi-strain network reconstruction. Author summary: The emergence of multidrug-resistant bacteria, particularly carbapenem-resistant Acinetobacter baumannii, has become a severe global health threat. This pressing issue necessitated the development of new antibiotics, as highlighted by the World Health Organization. To address this need, we aimed to create comprehensive metabolic models to better understand bacterial metabolism and aid in developing novel therapeutic strategies. In this study, we developed a versatile workflow to construct high-quality, simulation-ready genome-scale metabolic models for bacterial pathogens. Applying this workflow, we constructed a metabolic model for A. baumannii and validated its accuracy using experimental data. The model successfully replicated observed metabolic phenotypes and identified essential genes without human counterparts, suggesting potential targets for new antibiotics. Additionally, we assembled and curated the first collection of metabolic reconstructions for distinct A. baumannii strains, analyzing their growth characteristics. These standardized and well-curated models enhance usability, facilitating multi-strain network reconstruction and further research. These findings provide a robust tool for understanding A. baumannii's metabolism, guiding the development of new antimicrobial therapies. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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