1. Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure
- Author
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Lopez-Labrador, F.X., Brown, J.R., Fischer, N., Harvala, H., Boheemen, S. van, Cinek, O., Sayiner, A., Madsen, T.V., Auvinen, E., Kufner, V., Huber, M., Rodriguez, C., Jonges, M., Honemann, M., Susi, P., Sousa, H., Klapper, P.E., Perez-Cataluna, A., Hernandez, M., Molenkamp, R., Hoek, L. van der, Schuurman, R., Couto, N., Leuzinger, K., Simmonds, P., Beer, M., Hoper, D., Kamminga, S., Feltkamp, M.C.W., Rodriguez-Diaz, J., Keyaerts, E., Nielsen, X.C., Puchhammer-Stockl, E., Kroes, A.C.M., Buesa, J., Breuer, J., Claas, E.C.J., Vries, J.J.C. de, ESCV Network Next-Generation Seque, Virology, Medicum, Eeva Auvinen / Principal Investigator, Biosciences, HUSLAB, Department of Virology, University of Helsinki, and Helsinki University Hospital Area
- Subjects
0301 basic medicine ,Pathogen detection ,Standardization ,Computer science ,030106 microbiology ,Recommendations ,INFLUENZA-A VIRUS ,DIAGNOSIS ,VALIDATION ,DNA sequencing ,03 medical and health sciences ,0302 clinical medicine ,SDG 3 - Good Health and Well-being ,Virology ,Wet lab ,Viral metagenomics ,030212 general & internal medicine ,11832 Microbiology and virology ,Laboratory methods ,High-throughput sequencing ,Quality assessment ,Network on ,High-Throughput Nucleotide Sequencing ,DNA ,EFFICIENT TRANSLATION ,Data science ,3. Good health ,Infectious Diseases ,Metagenomics ,Viruses ,Next-generation sequencing ,3111 Biomedicine ,DEPLETION ,MESSENGER-RNA ,Clinical virology ,PATHOGEN DETECTION - Abstract
Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in a variety of sample types and infectious syndromes. mHTS is still in its early stages of translating into clinical application. To support the development, implementation and standardization of mHTS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mHTS for viral diagnostics to share methodologies and experiences, and to develop application recommendations. This manuscript aims to provide practical recommendations for the wet lab procedures necessary for implementation of mHTS for virus diagnostics and to give recommendations for development and validation of laboratory methods, including mHTS quality assurance, control and quality assessment protocols.
- Published
- 2021
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