1. Wastewater monitoring of SARS‐CoV‐2 gene for COVID‐19 epidemiological surveillance in Tucumán, Argentina.
- Author
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D'Arpino, María Cecilia, Sineli, Pedro Eugenio, Goroso, Gustavo, Watanabe, William, Saavedra, María Lucila, Hebert, Elvira María, Martínez, María Alejandra, Migliavacca, Julieta, Gerstenfeld, Silvina, Chahla, Rossana Elena, Bellomio, Augusto, and Albarracín, Virginia Helena
- Subjects
SARS-CoV-2 ,COVID-19 ,BIOMARKERS ,VIRAL load ,POLYMERASE chain reaction ,CITIES & towns - Abstract
Wastewater‐based epidemiology provides temporal and spatial information about the health status of a population. The objective of this study was to analyze and report the epidemiological dynamics of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) in the province of Tucumán, Argentina during the second and third waves of coronavirus disease 2019 (COVID‐19) between April 2021 and March 2022. The study aimed to quantify SARS‐CoV‐2 RNA in wastewater, correlating it with clinically reported COVID‐19 cases. Wastewater samples (n = 72) were collected from 16 sampling points located in three cities of Tucumán (San Miguel de Tucumán, Yerba Buena y Banda del Río Salí). Detection of viral nucleocapsid markers (N1 gene) was carried out using one‐step reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR). Viral loads were determined for each positive sample using a standard curve. A positive correlation (p < 0.05) was observed between viral load (copies/mL) and the clinically confirmed COVID‐19 cases reported at specific sampling points in San Miguel de Tucumán (SP4, SP7, and SP8) in both months, May and June. Indeed, the high viral load concurred with the peaks of COVID‐19 cases. This method allowed us to follow the behavior of SARS‐CoV‐2 infection during epidemic outbreaks. Thus, wastewater monitoring is a valuable epidemiological indicator that enables the anticipation of increases in COVID‐19 cases and tracking the progress of the pandemic. SARS‐CoV‐2 genome‐based surveillance should be implemented as a routine practice to prepare for any future surge in infections. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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