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The article reports the development of nested Reverse Transcriptase Polymerase Chain Reaction way to detect avian metapneumovirus, a virus that infects poultry chickens. It states according to Genbank, a nucleotide sequence database, two primers were prepared. It mentions that a particular base pair was amplified from the RNA of metapneumovirus though it was negative for avian influenza virus (AIV), Newcastle disease virus (NDV) and bronchitis virus (IBV).

The article focuses on the pathogenecity of Ornithobacterium rhinotracheale (ORT) on specific-pathogen free (SPF) chicken. It mentions that SPF chicks were artificially infected through the trachea, digestive tract and intramuscular challenge. The study reveals that ORT is mostly associated with tracheal infections and the target organs of ORT are lungs and trachea.

The article offers information on a study which examines the purification and characterization of the protease from Actinobacillus pleuropneumoniae serotype 7 (APS7). It notes that APS7, which was isolated from Shandong province in China, is cultivated in the lysogeny broth (LB) medium for 48 hours. It mentions that the purified protease indicated that its activity could be inhibited by ethylenediaminetetraacetic acid (EDTA).

The article offers information on the investigation of immunodepressive viruses including Marek's disease virus (MDV), reticuloendotheliosis virus (REV), and chicken anemia virus (CAV) which were obtained from the liver samples of 102 chickens with phymatosis from the provinces of Dongying, Rizhao, Weifang, and Liaocheng in Shandong, China.