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1. Esca‐affected grapevine leaf metabolome is clone‐ and vintage‐dependent

Author

Gaël Delorme, Florian Moret, Sophie Trouvelot, Christelle Lemaître-Guillier, Gilles Clément, Florence Fontaine, Marielle Adrian, Claire Grosjean, Agroécologie [Dijon], Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Chambre Régionale d’Agriculture de Bourgogne Franche-Comté (CRA BFC), Institut Jean-Pierre Bourgin (IJPB), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Bureau Interprofessionnel des Vins de Bourgogne, European Commission, Region Bourgogne-Franche-Comte, and Comite Champagne

Subjects
0106 biological sciences, 0301 basic medicine, Vintage, Physiology, [SDV]Life Sciences [q-bio], Metabolite, Clone (cell biology), Plant Science, Biology, 01 natural sciences, Vineyard, 03 medical and health sciences, chemistry.chemical_compound, Metabolomics, Genetics, Metabolome, Vitis, Cultivar, Plant Diseases, Disease expression, food and beverages, Cell Biology, General Medicine, Clone Cells, Plant Leaves, Horticulture, 030104 developmental biology, chemistry, 010606 plant biology & botany
Abstract

International audience; Esca is a complex grapevine trunk disease caused by wood-rotting ascomycetes and basidiomycetes and leading to several foliar and wood symptoms. Given that the esca expression can be influenced by several environmental, physiological, and genetic factors, foliar symptoms are inconsistent in incidence and prevalence and may appear 1 year but not the following. We have previously reported a clone-dependent expression of the disease in cv Chardonnay. Owing to metabolome analysis, we could discriminate the metabolite fingerprint of green leaves collected on diseased vines of clones 76 and 95. These clone-dependent fingerprints were year-dependent in intensity and nature. The present work was conducted to determine if the clone-dependent disease expression observed is specific to Chardonnay or if it also occurs in another cultivar. A plot located in the Jura vineyard (France) and planted with both 1004 and 1026 clones of Trousseau, a cultivar highly susceptible to esca, was thus selected and studied during 2017 and 2018. A year-dependent variation of the symptoms expression was first observed and a possible relationship with rainfall is hypothesized and discussed. Moreover, a higher percentage of the clone 1026 vines expressed disease, compared to the 1004 ones, suggesting the higher susceptibility of this clone. Finally, metabolomic analyses of the remaining green leaves (i.e, without symptom expression) of partial esca-apoplectic vines allowed us to confirm a clone-dependent metabolic response to the disease. The metabolite fingerprints obtained differed in nature and intensity to those previously reported for Chardonnay and also between years.

Published
2020
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2. In vitro inhibition of shikimate hydroxycinnamoyltransferase by acibenzolar acid, the first metabolite of the plant defence inducer acibenzolar-S-methyl

Author

Jonathan Negrel, Marielle Adrian, Agnès Klinguer, Agroécologie [Dijon], and Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
0106 biological sciences, 0301 basic medicine, Acibenzolar, Physiology, Stereochemistry, Metabolite, [SDV]Life Sciences [q-bio], shikimate hydroxycinnamoyltransferase, Quinic Acid, Shikimic Acid, Plant Science, 01 natural sciences, 03 medical and health sciences, chemistry.chemical_compound, acibenzolar acid, Thiadiazoles, Tobacco, Genetics, Inducer, Binding site, chemistry.chemical_classification, enzyme promiscuity, Quinic acid, 3-Mercaptobenzoic acid, 030104 developmental biology, Enzyme, chemistry, acibenzolar-S-Methyl, Putrescine, Acibenzolar-S-methyl, 010606 plant biology & botany
Abstract

International audience; Acibenzolar acid, the first metabolite formed in planta from the defence inducer acibenzolar-S-methyl (ASM), has been shown to be an inhibitor of the enzyme shikimate hydroxycinnamoyltransferase (HST), extracted from grapevine or tobacco cell suspension cultures. Using a purified recombinant Arabidopsis thaliana HST, the inhibition was found to be competitive, acibenzolar acid binding reversibly to the shikimate binding site of the HST:p-coumaroyl-CoA complex, with a Ki value of 250 μM. The other hydroxycinnamoyltransferases tested in the course of this study, using either hydroxypalmitic acid, putrescine, tyramine, or quinic acid as acyl acceptors were not, or only slightly, inhibited by acibenzolar acid. To understand the specificity of the interaction of acibenzolar acid with HST, we analyzed the structure-activity relationship of a series of benzoic or acibenzolar acid analogues, tested either as AtHST substrates or as inhibitors. This analysis confirmed previously published data on the substrate flexibility of HST and demonstrated that both the carboxyl group and the thiadiazole moiety of acibenzolar acid are playing an important role in the interaction with the shikimate binding site. Acibenzolar acid, which cannot form an ester bond with p-coumaric acid, was however a less potent inhibitor than protocatechuic or 3-hydroxybenzoic acids, which are used as acyl acceptors by HST. Our results show that the interaction of acibenzolar acid with HST, which is probably directly linked to the substrate promiscuity of HST, is unlikely to play a direct role in the defence-inducing properties of ASM in plants.

Published
2021
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3. Proteomics towards the understanding of elicitor induced resistance of grapevine against downy mildew

Author

Christelle Lemaître-Guillier, Benoît Poinssot, Christine Schaeffer-Reiss, Sophie Trouvelot, Marielle Adrian, Agnès Hovasse, Xavier Daire, Marie-Claire Héloir, Ghislaine Recorbet, Agroécologie [Dijon], Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté ( UBFC ), UMR 7178 Institut pluridisciplinaire Hubert Curien (IPHC) - Laboratoire de Spectrométrie de Masse BioOrganique, Centre National de la Recherche Scientifique ( CNRS ), Université de Strasbourg ( UNISTRA ), BIVB (Bureau Interprofessionnel des Vins de Bourgogne), Conseil Regional de Bourgogne [2013 - 9201AA0050S02328], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Laboratoire de Spectrométrie de Masse BioOrganique [Strasbourg] (LSMBO), Département Sciences Analytiques et Interactions Ioniques et Biomoléculaires (DSA-IPHC), Institut Pluridisciplinaire Hubert Curien (IPHC), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut Pluridisciplinaire Hubert Curien (IPHC), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), and Centre National de la Recherche Scientifique (CNRS)

Subjects
Proteomics, 0106 biological sciences, 0301 basic medicine, [SDV]Life Sciences [q-bio], Biophysics, Laminarin, 01 natural sciences, Biochemistry, Plasmopara viticola, 03 medical and health sciences, Gene Expression Regulation, Plant, Botany, Plant defense against herbivory, Vitis, Leaf proteome, Glucans, Pathogen, Disease Resistance, Plant Diseases, Plant Proteins, 2. Zero hunger, Peronospora, [ SDV ] Life Sciences [q-bio], biology, food and beverages, Induced resistance, Biotic stress, 2-DE, biology.organism_classification, Elicitor, Plant Leaves, Fungicide, 030104 developmental biology, Vitis vinifera, Downy mildew, Electrophoresis, Polyacrylamide Gel, Resistance Process, 010606 plant biology & botany
Abstract

Elicitors are known to trigger plant defenses in response to biotic stress, but do not systematically lead to effective resistance to pathogens. The reasons explaining such differences remain misunderstood. Therefore, elicitation and induced resistance (IR) were investigated through the comparison of two modified β-1,3 glucans applied on grapevine (Vitis vinifera) leaves before and after inoculation with Plasmopara viticola, the causal agent of downy mildew. The sulfated (PS3) and the shortened (H13) forms of laminarin are both known to elicit defense responses whereas only PS3 induces resistance against downy mildew. The analysis of the 2-DE gel electrophoresis revealed that PS3 and H13 induced distinct proteomic profiles after treatment and pathogen inoculation. Our results point out that the PS3-induced resistance is associated with the activation of the primary metabolism especially on amino acids and carbohydrates pathways. In addition, few proteins, such as the 12-oxophytodienoate reductase (OPR-like) related to the OPDA pathway, and an Arsenite-resistance protein (Serrate-like protein) could be considered as useful markers of induced resistance. Significance One strategy to reduce the application of fungicides is the use of elicitors which induce plant defense responses. Nonetheless, the elicitors do not systematically lead to resistance against pathogens. The lack of correlation between plant defense activation and induced resistance (IR) requires the investigation of what makes the specificity of elicitor-IR. In this study, the two β-glucans elicitors, sulfated (PS3) and short (H13) laminarins, were used in the grapevine/Plasmopara viticola interaction since only the first one leads to resistance against downy mildew. To disclose IR specificity, proteomic approach has been employed to compare the two treatments before and after P. viticola inoculation. The analysis of the 2-DE revealed that PS3 and H13 induced distinct proteomic profiles after treatment and pathogen inoculation. Significant increase of the number of proteins regulated by PS3, relative to both H13 and time-points, is correlated with the resistance process establishment. Our results point that the PS3-induced resistance requires the activation of the primary metabolism especially on amino acids and carbohydrates pathways. In addition, few proteins, such as the 12-oxophytodienoate reductase (OPR-like) related to the OPDA pathway, and an Arsenite-resistance protein (Serrate-like protein) could constitute useful markers of PS3 induced resistance.

Published
2017
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4. Recognition of Elicitors in Grapevine: From MAMP and DAMP Perception to Induced Resistance

Author

Marie-Claire Héloir, Marielle Adrian, Daphnée Brulé, Justine Claverie, Sylvain Cordelier, Xavier Daire, Stéphan Dorey, Adrien Gauthier, Christelle Lemaître-Guillier, Jonathan Negrel, Lucie Trdá, Sophie Trouvelot, Elodie Vandelle, Benoit Poinssot, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Unité de Recherche Vigne et Vins de Champagne Stress et Environnement - EA 4707 (URVVC), Plante - microbe - environnement : biochimie, biologie cellulaire et écologie (PMEBBCE), Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Institute of Experimental Botany ASCR, Poinssot, Benoît, Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD), Institute of Experimental Botany of the Czech Academy of Sciences (IEB / CAS), Czech Academy of Sciences [Prague] (CAS), ANRFrench National Research Agency (ANR), FederEuropean Union (EU), Regional Council of Bourgogne Franche-ComteRegion Bourgogne-Franche-Comte, Bureau Interprofessionnel des Vins de Bourgogne, and MESRI (Ministere de l'Enseignement Superieur, de la Recherche et de l'Innovation)

Subjects
0106 biological sciences, 0301 basic medicine, Damp, Vitis vinifera, innate immunity, defense responses, Pattern Recognition Receptor (PRR), MicrobeAssociated Molecular Pattern (MAMP), Damage-Associated Molecular Pattern (DAMP), Induced Resistance (IR), [SDV]Life Sciences [q-bio], MicrobeAssociated Molecular Pattern (MAMP), Context (language use), Plant Science, Review, Biology, lcsh:Plant culture, 01 natural sciences, Cell wall, 03 medical and health sciences, Microbe-Associated Molecular Pattern (MAMP), Plant defense against herbivory, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, lcsh:SB1-1110, MAMP, innate immunity, Damage-Associated Molecular Pattern (DAMP), ComputingMilieux_MISCELLANEOUS, 2. Zero hunger, Innate immune system, fungi, defense responses, induced resistance (IR), damage-associated molecular pattern (damp), microbe-associated molecular pattern (mamp), pattern recognition receptor (prr), vitis vinifera, Pattern recognition receptor, food and beverages, Induced Resistance (IR), Elicitor, Cell biology, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, 030104 developmental biology, Vitis vinifera, Pattern Recognition Receptor (PRR), 010606 plant biology & botany
Abstract

International audience; In a context of a sustainable viticulture, the implementation of innovative eco-friendly strategies, such as elicitor-triggered immunity, requires a deep knowledge of the molecular mechanisms underlying grapevine defense activation, from pathogen perception to resistance induction. During plant-pathogen interaction, the first step of plant defense activation is ensured by the recognition of microbe-associated molecular patterns, which are elicitors directly derived from pathogenic or beneficial microbes. Vitis vinifera, like other plants, can perceive elicitors of different nature, including proteins, amphiphilic glycolipid, and lipopeptide molecules as well as polysaccharides, thanks to their cognate pattern recognition receptors, the discovery of which recently began in this plant species. Furthermore, damage-associated molecular patterns are another class of elicitors perceived by V. vinifera as an invader's hallmark. They are mainly polysaccharides derived from the plant cell wall and are generally released through the activity of cell wall-degrading enzymes secreted by microbes. Elicitor perception and subsequent activation of grapevine immunity end in some cases in efficient grapevine resistance against pathogens. Using complementary approaches, several molecular markers have been identified as hallmarks of this induced resistance stage. This review thus focuses on the recognition of elicitors by Vitis vinifera describing the molecular mechanisms triggered from the elicitor perception to the activation of immune responses. Finally, we discuss the fact that the link between elicitation and induced resistance is not so obvious and that the formulation of resistance inducers remains a key step before their application in vineyards.

Published
2019
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5. Surfactin and fengycin contribute to the protection of a Bacillus subtilis strain against grape downy mildew by both direct effect and defence stimulation

Author

Lucile Jacquens, Marius Henkel, Qi Wang, Marielle Adrian, Xuewen Fang, Xun Zhang, Marie-Claire Héloir, Sophie Trouvelot, Mareen Geissler, Xin Su, Yan Li, China Agricultural University (CAU), Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, and University of Hohenheim

Subjects
0106 biological sciences, 0301 basic medicine, defence, [SDV]Life Sciences [q-bio], Mutant, Soil Science, Plant Science, Bacillus subtilis, 01 natural sciences, Peptides, Cyclic, surfactin, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, Lipopeptides, Plasmopara viticola, Gene Expression Regulation, Plant, Vitis, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Molecular Biology, Pathogen, Glucans, Plant Diseases, Oomycete, Peronospora, biology, downy mildew, Callose, fengycin, food and beverages, Original Articles, biology.organism_classification, Plant Leaves, 030104 developmental biology, chemistry, lipopeptide, Downy mildew, Original Article, Surfactin, Agronomy and Crop Science, 010606 plant biology & botany
Abstract

International audience; Bacillus subtilis GLB191 (hereafter GLB191) is an efficient biological control agent against the biotrophic oomycete Plasmopara viticola, the causal agent of grapevine downy mildew. In this study, we show that GLB191 supernatant is also highly active against downy mildew and that the activity results from both direct effect against the pathogen and stimulation of the plant defences (induction of defence gene expression and callose production). High-performance thin-layer chromatography analysis revealed the presence of the cyclic lipopeptides fengycin and surfactin in the supernatant. Mutants affected in the production of fengycin and/or surfactin were thus obtained and allowed us to show that both surfactin and fengycin contribute to the double activity of GLB191 supernatant against downy mildew. Altogether, this study suggests that GLB191 supernatant could be used as a new biocontrol product against grapevine downy mildew.

Published
2019
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6. The grapevine (Vitis vinifera) LysM receptor kinases VvLYK1-1 and VvLYK1-2 mediate chitooligosaccharide-triggered immunity

Author

Justine Claverie, Freddy Boutrot, Lena Stransfeld, Daphnée Brulé, Lucie Trdá, Marie-Claire Héloir, Pablo Tornero, Laura J. Davies, Clizia Villano, Annick Chiltz, Benoît Darblade, Marielle Adrian, Cyril Zipfel, Benoît Poinssot, Ian B. Dry, University of Zurich, Dry, Ian B, Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Centre National de la Recherche Scientifique (CNRS), University of Naples Federico II, Commonwealth Scientific and Industrial Research Organisation [Canberra] (CSIRO), Elicityl SA, Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Norwich Research Park, The Sainsbury Laboratory [Norwich] (TSL), ANR (PATRIC project) [ANR-13-KBBE-0001], Regional Council of Bourgogne Franche-Comte (PARI grant) [2016-9201AAO050S016 36, 2015-9201AAO048502578, 29000907], Regional Council of Bourgogne Franche-Comte (FEDER) [2015-9201AAO048502578, 29000907, BG0005888], INRA [2015-9201AAO048502578, 29000907], Gatsby Charitable Foundation, Biotechnology and Biological Sciences Research Council (BBSRC) [BB/G024936/1], Dry, Ian B., Poinssot, Benoît, Brule, Daphnee, Villano, Clizia, Davies, Laura J., Trda, Lucie, Claverie, Justine, Heloir, Marie-Claire, Chiltz, Annick, Adrian, Marielle, Darblade, Benoıt, Tornero, Pablo, Stransfeld, Lena, Boutrot, Freddy, Zipfel, Cyril, and Poinssot, Benoit

Subjects
0106 biological sciences, 0301 basic medicine, pattern recognition receptor, [SDV]Life Sciences [q-bio], Resistance, Mutant, Arabidopsis, Oligosaccharides, Chitin, Immune responses, Plant Science, 580 Plants (Botany), Erysiphe necator, 01 natural sciences, immune response, chemistry.chemical_compound, 10126 Department of Plant and Microbial Biology, Gene expression, 1110 Plant Science, Plant Immunity, Vitis, pathogen-associated molecular pattern, Vitis vinifera, immune responses, resistance, Research Articles, Phylogeny, Plant Proteins, 2. Zero hunger, Pattern recognition receptor, Cell biology, Elicitor, [SDE]Environmental Sciences, 1305 Biotechnology, Research Article, Biotechnology, macromolecular substances, Pathogen-associated molecular pattern, Biology, pathogen‐associated molecular pattern, Protein Serine-Threonine Kinases, 03 medical and health sciences, Ascomycota, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, 1102 Agronomy and Crop Science, 10211 Zurich-Basel Plant Science Center, Gene, Plant Diseases, Chitosan, Arabidopsis Proteins, fungi, 15. Life on land, biology.organism_classification, 030104 developmental biology, chemistry, Agronomy and Crop Science, 010606 plant biology & botany
Abstract

[EN] Chitin, a major component of fungal cell walls, is a well-known pathogen-associated molecular pattern (PAMP) that triggers defense responses in several mammal and plant species. Here, we show that two chitooligosaccharides, chitin and chitosan, act as PAMPs in grapevine (Vitis vinifera) as they elicit immune signalling events, defense gene expression and resistance against fungal diseases. To identify their cognate receptors, the grapevine family of LysM receptor kinases (LysM-RKs) was annotated and their gene expression profiles were characterized. Phylogenetic analysis clearly distinguished three V. vinifera LysM-RKs (VvLYKs) located in the same clade as the Arabidopsis CHITIN ELICITOR RECEPTOR KINASE1 (AtCERK1), which mediates chitin-induced immune responses. The Arabidopsis mutant Atcerk1, impaired in chitin perception, was transformed with these three putative orthologous genes encoding VvLYK1-1, -2, or -3 to determine if they would complement the loss of AtCERK1 function. Our results provide evidence that VvLYK1-1 and VvLYK1-2, but not VvLYK1-3, functionally complement the Atcerk1 mutant by restoring chitooligosaccharide-induced MAPK activation and immune gene expression. Moreover, expression of VvLYK1-1 in Atcerk1 restored penetration resistance to the non-adapted grapevine powdery mildew (Erysiphe necator). On the whole, our results indicate that the grapevine VvLYK1-1 and VvLYK1-2 participate in chitin- and chitosan-triggered immunity and that VvLYK1-1 plays an important role in basal resistance against E. necator., This work has been financially supported by ANR (PATRIC project, grant ANR-13-KBBE-0001) (to BP), the Regional Council of Bourgogne Franche-Comte (PARI grant 2016-9201AAO050S016 36 and FEDER grant BG0005888) and INRA for the funding of Justine Claverie's PhD (grants 2015-9201AAO048502578 and 29000907), by the Gatsby Charitable Foundation (to CZ), and by the Biotechnology and Biological Sciences Research Council (BBSRC) grants BB/G024936/1 (ERA-PG PRR CROP (to CZ).

Published
2019
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7. Clone-Dependent Expression of Esca Disease Revealed by Leaf Metabolite Analysis

Author

Sophie Trouvelot, Christian Coelho, Marielle Adrian, Gilles Clément, Florence Fontaine, Guillaume Morvan, Claire Grosjean, Jonathan Negrel, Florian Moret, Christelle Lemaître-Guillier, Lucile Jacquens, Grégory Mouille, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Chambre d’Agriculture de Bourgogne Franche-Comté, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Institut Universitaire de la Vigne et du Vin 'Jules Guyot' (IUVV Jules Guyot), Université de Bourgogne (UB), Procédés Alimentaires et Microbiologiques [Dijon] (PAM), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Chambre d'Agriculture de l'Yonne (CA 89), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Adrian, Marielle, Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, and European Union (Feder program), Region Bourgogne Franche Comte, Bureau Interprofessionnel des Vins de Bourgogne

Subjects
0106 biological sciences, 0301 basic medicine, [SDV]Life Sciences [q-bio], Esca, Clone (cell biology), Context (language use), Disease, Plant Science, Biology, lcsh:Plant culture, 01 natural sciences, 03 medical and health sciences, Metabolomics, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Metabolome, lcsh:SB1-1110, Cultivar, grapevine trunk diseases, ComputingMilieux_MISCELLANEOUS, Original Research, 2. Zero hunger, clone, food and beverages, Metabolite analysis, metabolomics, Vitis vinifera, 3D fluorescence, Horticulture, 030104 developmental biology, Shoot, [SDV.IB]Life Sciences [q-bio]/Bioengineering, 010606 plant biology & botany
Abstract

International audience; Grapevine trutk diseases, especially Esca, are of major concern since they gradually alter vineyards worldwide and cause heavy economic losses. The expression of Esca disease symptoms depends on several factors, including the grapevine cultivar. In this context, a possible clone-dependent expression of the Esca disease was studied. Two clones of 'Chardonnay' grown in the same plot were compared according to their developmental and physiological traits, metabolome, and foliar symptom expression. Analysis of their leaf metabolome highlighted differences related to symptom expression. Interestingly, the content of a few specific metabolites exhibited opposite variations in leaves of symptomatic shoots of clones 76 and 95. Altogether this study showed a clone-dependent expression of Esca disease in 'Chardonnay' and the relevance of GC-MS and 3D fluorescence methods to analyze the impact of the disease on the leaf metabolome.

Published
2019
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8. L’holobionte « vigne » face au dépérissement ou quand le « vivre ensemble » n’est plus à l’équilibre

Author

Sophie TROUVELOT, Héloise Mahé, Pauline Bettenfeld, Evert van Schaik, Marielle Adrian, François Dal, Olivier Fernandez, Florence Fontaine, Claire Grosjean, Philippe Larignon, Gregory Mouille, Fabrice Martin, Sylvain Pabion, Olivier Zekri, Samuel Mondy, Daniel Wipf, Pierre-Emmanuel Courty, ProdInra, Migration, Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Comité National des Interprofessions des Vins, Partenaires INRAE, Université de Reims Champagne-Ardenne (URCA), Sicavac, Chambre Régionale d’Agriculture de Bourgogne Franche-Comté (CRA BFC), Institut Français de la Vigne et du Vin (IFV), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Château de Marsannay, and Pépinières Mercier Frères

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology
Abstract

Prod 2019-165 SPE EA IPM BIOmE GenoSol INRA UB; National audience

Published
2019

9. ) Clone-dependent expression of grapevine esca disease

Author

Florian Moret, Christelle Guillier, Claire Grosjean, Gilles Clément, Christian Coelho, Lucile Jacquens, Jonathan Negrel, Régis Gougeon, Guillaume Morvan, Gregory Mouille, Sophie TROUVELOT, Florence Fontaine, Marielle Adrian, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Chambre d’Agriculture de Bourgogne Franche-Comté, Institut Universitaire de la Vigne et du Vin 'Jules Guyot' (IUVV Jules Guyot), Université de Bourgogne (UB), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Universidad de la Rioja. Rioja, ESP., Chambre d'Agriculture de l'Yonne (CA 89), Université de Reims Champagne-Ardenne (URCA), Université de Bourgogne Franche-Comté (COMUE) (UBFC). FRA., University of British Columbia (UBC). CAN., and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.IDA]Life Sciences [q-bio]/Food engineering, food and beverages, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.IDA] Life Sciences [q-bio]/Food engineering, ComputingMilieux_MISCELLANEOUS
Abstract

International audience; The occurrence of grapevine trunk diseases (GTDs) increases gradually within vineyards, leading to important economic losses. Several factors could affect grapevine susceptibility to GTDs, especially climate, vine age, soil fertilization, and also rootstock, cultivar and clone. For a cultivar, the level of disease expression can vary with region and from year to year. In this context, our objective was to assess if the expression of esca disease, one of the most widespread GTDs, was also clone-dependent. Two clones (76 and 95) of the “Chardonnay” cultivar, grown in the same plot, were compared according to their developmental and physiological traits, metabolome, and esca foliar symptom expression. Agronomical data and symptom expression were recorded during summer 2015, and metabolome analyses were performed in leaf samples collected from visually healthy vines as control (C), and from both symptomatic (D+) and asymptomatic (D-) shoots of esca-affected vines. The bud burst percentage and the fertility ratio were significantly lower for clone 76 than for clone 95, whereas the plant vigor was similar. The percentage of vines expressing the apoplectic and chronic forms of esca disease was low but slightly higher for clone 95. Global GC-MS analysis highlighted a clone-dependent metabolic fingerprint of disease expression. Additional targeted HPLC analyses showed opposite variations in the accumulation of trans-caffeoyltartaric acid, quercetin-O-glucoside, quercetin-O-galactoside, and kaempferol-O-glucoside (higher levels in control leaves of clone 76 than in diseased ones, and the opposite for clone 95). An original approach, 3D fluorescent analysis, was also used to compare samples and also pointed out significant differences in disease expression between clones. Altogether, this study highlights a clone-dependent metabolic response related to esca-disease expression, and the potential of the 3D fluorescence analysis as a new method to detect it. It would be interesting to extend the analysis to other clones and varieties.

Published
2019

10. Dual mode of action of grape cane extracts against Botrytis cinerea

Author

Benoît Poinssot, Annick Chiltz, Marie-Claire Héloir, Jonathan Negrel, Marielle Adrian, Agnès Klinguer, Gicele Sbardelotto De Bona, Simone Vincenzi, Elisa Angelini, Nadia Bertazzon, Universitad de Padua, Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), Universita degli Studi di Padova, Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Centre National de la Recherche Scientifique (CNRS), Consiglio per la Ricerca in Agricoltura e l’analisi dell’economia agraria (CREA), National Scientific and Technological Development Council (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico) (CNPq/Brazil) [206331/2014-2], and University of Padua

Subjects
[SDV]Life Sciences [q-bio], Resveratrol, resveratrol, stilbenes, chemistry.chemical_compound, Botrytis cinerea, Gene Expression Regulation, Plant, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Vitis, Cane, Vitis vinifera, Plant Diseases, Plant Proteins, chemistry.chemical_classification, phytoalexin, biology, Mycelium, Plant Stems, Plant Extracts, Phytoalexin, fungi, Dual mode, food and beverages, General Chemistry, defense response, cane extract, biology.organism_classification, Fungicides, Industrial, grapevine, Plant Leaves, Horticulture, chemistry, [SDE]Environmental Sciences, Natural source, Botrytis, General Agricultural and Biological Sciences
Abstract

International audience; Crude extracts of Vitis vinifera canes represent a natural source of stilbene compounds with well characterized antifungals properties. In our trials, exogenous application of a stilbene extract (SE) obtained from grape canes on grapevine leaves reduces the necrotic lesions caused by Botrytis cinerea. The SE showed to possess a direct antifungal activity by inhibiting the mycelium growth. The activation of some grapevine defense mechanism was also investigated. H2O2 production and activation of mitogen-activated protein kinase (MAPK) phosphorylation cascades as well as accumulation of stilbenoid phytoalexins were explored on grapevine cell suspension. Moreover, the transcription of genes encoding for proteins affecting defense responses was analyzed on grapevine plants. The SE induced some grapevine defense mechanisms including MAPK activation, and the expression of pathogenesis-related (PR) genes and of a gene encoding the glutathione-S-transferase 1 (GST1). By contrast, treatment of grapevine leaves with SE negatively regulates de novo stilbene production.

Published
2019
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11. L’holobionte vigne, un levier biologique pour lutter contre les dépérissements ?

Author

Sophie TROUVELOT, Héloise Mahé, Pauline Bettenfeld, Evert van Schaik, Marielle Adrian, François Dal, Olivier Fernandez, Florence Fontaine, Claire Grosjean, Philippe Larignon, Gregory Mouille, Fabrice Martin-Laurent, Sylvain Pabion, Olivier Zekri, Samuel Mondy, Daniel Wipf, Pierre-Emmanuel Courty, Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Comité National des Interprofessions des Vins, Partenaires INRAE, SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Sicavac, Chambre régionale d'agriculture, Institut Français de la Vigne et du Vin (IFV), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Château de Marsannay, Pépinières Mercier Frères, and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.IDA] Life Sciences [q-bio]/Food engineering, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2019

12. Expression clone-dépendante de l’Esca chez la vigne

Author

Florian Moret, Christelle Guillier, Claire Grosjean, Gilles Clément, Christia Coelho, Lucile JACQUENS, Jonathan Negrel, Régis Gougeon, Guillaume Morvan, Gregory Mouille, Sophie Trouvelot, Florence Fontaine, Marielle Adrian, Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Chambre d’Agriculture de Bourgogne Franche-Comté, Institut National de la Recherche Agronomique (INRA), Université de Bourgogne (UB), Université de Montpellier (UM), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université de Reims Champagne-Ardenne (URCA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.IDA] Life Sciences [q-bio]/Food engineering
Abstract

National audience; L’incidence des maladies du bois de la vigne (MDB) augmente graduellement au sein des vignobles, causant d’importantes pertes économiques. Le coût mondial du remplacement de vignes malades est estimé à plus d’1,5 milliards de dollars par an [1]. En France, presque 13% du vignoble est affecté [2]. Plusieurs facteurs pourraient influencer la sensibilité aux MDB, notamment le climat, l’âge de la vigne, la fertilisation du sol, le porte-greffe ainsi que le cépage [3]. Actuellement, aucun cépage tolérant aux MDB n’a pu être identifié et le niveau de l’expression des MDB pour un cépage donné peut varier en fonction de la région et d’une année à l’autre [3], et probablement selon notre hypothèse en fonction du clone. Dans ce contexte, notre objectif a été de déterminer si l’expression de l’Esca, une des MDB les plus répandues, était clone-dépendante. Deux clones (76 et 95) du cépage Chardonnay plantés dans la même parcelle ont été comparés selon leurs traits développementaux et physiologiques, leur métabolome, et l’expression foliaire des symptômes d’Esca. Les paramètres agronomiques des vignes furent mesurés durant l’été 2015, et les analyses du métabolome ont été réalisées sur des échantillons de feuilles, dénuées de symptômes foliaires, prélevées sur des vignes asymptomatiques en tant que contrôle (C), et sur des rameaux symptomatiques (D+) et asymptomatiques (D-) de vignes exprimant l’Esca. Les données de GC-MS montrent que l’expression des symptômes d’Esca induit des changements dans le métabolome des feuilles. Certains métabolites présentaient un différentiel d’accumulation entre les feuilles C et D+ au sein de chaque clone. De plus, une différence de profil métabolique entre les feuilles C et D- n’a été observée que pour le clone 95. Enfin, certains métabolites sont inversement accumulés chez les 2 clones pour les échantillons de feuilles D+, avec des quantités plus faibles que chez le contrôle pour le clone 76 et plus élevées pour le clone 95. Il s’agit notamment du kaempferol, kaempferol-3-O-glucoside, gentiobiose et quercétine. La complexité de ces résultats met en avant la difficulté de détecter, chez le Chardonnay, une empreinte métabolique commune aux 2 clones testés associée à l’expression de l’Esca. Enfin, une analyse par fluorescence 3D a également permis de discriminer les échantillons D+ de chaque clone (et de leur contrôle), montrant ainsi le potentiel de cette méthodologie pour étudier l’impact de l’esca sur le métabolome foliaire. Ainsi, cette étude montre que des clones d’un même cépage peuvent répondre, d’un point de vue métabolique, de façon différente à une atteinte d’Esca. Elle sera prochainement publiée dans la revue Frontiers in Plant Science.

Published
2018

13. A Plant Extract Acts Both as a Resistance Inducer and an Oomycide Against Grapevine Downy Mildew

Author

Tristan Richard, Marie-Claire Héloir, Ambrine Bougaud, Jonathan Negrel, Sophie Trouvelot, Marielle Adrian, Lucile Jacquens, Agnès Klinguer, Annick Chiltz, Stéphanie Cluzet, Yuko Krzyzaniak, Josep Valls, Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Unité de Recherche Oenologie [Villenave d'Ornon], and Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)

Subjects
0106 biological sciences, 0301 basic medicine, plant protection, oomycide, Zoospore, [SDV]Life Sciences [q-bio], Biological pest control, Plant Science, lcsh:Plant culture, Resveratrol, 01 natural sciences, induced resistance, Plasmopara viticola, 03 medical and health sciences, chemistry.chemical_compound, [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, lcsh:SB1-1110, Inducer, biocontrol, Original Research, Piceid, elicitor, biology, food and beverages, biology.organism_classification, Elicitor, Horticulture, 030104 developmental biology, chemistry, Vitis vinifera, [SDE]Environmental Sciences, Downy mildew, 010606 plant biology & botany
Abstract

International audience; Protecting vineyards from cryptogamic diseases such as downy mildew, caused by Plasmopara viticola, generally requires a massive use of phytochemicals. However, the issues on unintentional secondary effects on environment and human health, and the occurrence of P. viticola resistant strains, are leading to the development of alternative strategies, such as the use of biocontrol products. In this paper, we evidenced the ability of a plant extract to protect grapevine from P. viticola. Further experiments carried out both on cell suspensions and on plants revealed that plant extract activates typical defense-related responses such as the production of H2O2, the up-regulation of genes encoding pathogenesis-related proteins and stilbene synthase, as well as the accumulation of resveratrol or its derivative piceid. We also brought to light a strong direct effect of PE on the release and motility of P. viticola zoospores. Furthermore, we found out that PE application left dried residues on leaf surface, impairing zoospores to reach stomata. Altogether, our results highlight the different modes of action of a new biocontrol product able to protect grapevine against downy mildew.

Published
2018
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14. Toward the Identification of Two Glycoproteins Involved in the Stomatal Deregulation of Downy Mildew–Infected Grapevine Leaves

Author

Christelle Guillier, Delphine Pecqueur, Marielle Adrian, Patrick Ducoroy, Marie-Claire Héloir, Caroline Truntzer, Magdalena Gamm, Géraldine Lucchi, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Université de Bourgogne (UB), Plate-forme Protéomique CLIPP - Clinical and Innovation Proteomic Platform [Dijon] (CLIPP), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)-Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie Moléculaire de l'Université de Bourgogne [Dijon] (ICMUB), Université de Bourgogne (UB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Conseil Regional de Bourgogne, Bureau Interprofessionnel des Vins de Bourgogne (BIVB), International Society for Horticultural Science (ISHS). INT., and ProdInra, Migration

Subjects
[SDE] Environmental Sciences, Proteomics, Physiology, [SDV]Life Sciences [q-bio], stomata, Molecular Sequence Data, Plant Epidermis, Fungal Proteins, Cell wall, Plasmopara, Plasmopara viticola, chemistry.chemical_compound, Cell Wall, Botany, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, Computer Simulation, Vitis, Amino Acid Sequence, Pathogen, Abscisic acid, Phylogeny, proteomic, Glycoproteins, Plant Diseases, Plant Proteins, plant-microbe interaction, Fungal protein, Sequence Homology, Amino Acid, biology, fungi, Plant Stomata, food and beverages, General Medicine, Chromatography, Ion Exchange, biology.organism_classification, Apoplast, [SDV] Life Sciences [q-bio], Plant Leaves, Oomycetes, Biochemistry, chemistry, Vitis vinifera, Host-Pathogen Interactions, [SDE]Environmental Sciences, Downy mildew, guard cell, Agronomy and Crop Science
Abstract

SPE Pôle IPM UB; International audience; Stomata remain abnormally opened and unresponsive to abscisic acid in grapevine leaves infected by downy mildew. This deregulation occurs from 3 days post inoculation and increases concomitantly with leaf colonization by the pathogen. Using epidermal peels, we demonstrated that the active compound involved in this deregulation is located in the apoplast. Biochemical assays showed that the active compound present in the apoplastic fluids isolated from Plasmopara viticola infected grapevine leaves (IAF) is a CysCys bridge-independent, thermostable and glycosylated protein. Fractionation guided assays based on chromatography / stomatal response and proteomic analysis allowed the identification of both plant and pathogen proteins in the active fraction obtained from IAF. Further in silico analysis and discriminant filtrations based on the comparison between predictions and experimental indications lead to the identification of two V. vinifera proteins as candidates for the observed stomatal deregulation

Published
2015
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15. Assessment of the impact of PS3-induced resistance to downy mildew on grapevine physiology

Author

Marielle Adrian, Eric Bernaud, Christelle Lemaître-Guillier, Ian Li Kim Khiook, Gilles Clément, Marie-Claire Héloir, Sophie Trouvelot, Lucile Jacquens, Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Université Bourgogne Franche-Comté [COMUE] (UBFC), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris Saclay (COmUE), Conseil Regional de Bourgogne Franche Comte, Bureau Interprofessionnel des Vins de Bourgogne (BIVB), and LabEx Saclay Plant Sciences-SPS [ANR-10-LABX-0040-SPS]

Subjects
0106 biological sciences, 0301 basic medicine, Physiology, [SDV]Life Sciences [q-bio], Context (language use), Plant Science, Biology, 01 natural sciences, 03 medical and health sciences, Plasmopara viticola, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Genetics, Metabolomics, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Vitis, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, Glucans, Disease Resistance, Plant Diseases, 2. Zero hunger, Primary metabolite, Plant physiology, food and beverages, Herbaceous plant, biology.organism_classification, Elicitor, Fungicide, Plant Leaves, 030104 developmental biology, Oomycetes, Vitis vinifera, [SDE]Environmental Sciences, Downy mildew, Elicitor-induced resistance, Grapevine physiology, 010606 plant biology & botany
Abstract

International audience; Elicitor-induced resistance against diseases is an attractive strategy that could contribute to reduce the use of fungicides for plant protection. However, activation of defenses has an energetic cost that plants have to fuel by a mobilization of their primary metabolism with possible adverse effect on their physiology. In this context, this study was performed to determine whether elicitor-induced resistance of grapevine leaves against downy mildew impacted its development and metabolism. The elicitor PS3 (sulfated beta-glucan laminarin) was sprayed on grapevine herbaceous cuttings grown in greenhouses once or three times, and its impact was studied on young and older grapevine leaves, prior to, and after Plasmopara viticola inoculation. PS3 did not affect grapevine development during the time course of the experiment. A metabolomic analysis, mainly focused on primary metabolites, highlighted a leaf age dependent effect of PS3 treatment. Nitrogen compounds, and sugars to a lesser extent, were impacted. The results obtained complete the current knowledge of the impact of elicitor-induced resistance on plant physiology. They will be helpful to guide further experiments required to better determine the costs and benefits of elicitor-induced resistance in plants.

Published
2018
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16. Mycorhization et viticulture

Author

Alice Drain, Diederik van Tuinen, Eric Bernaud, Sophie TROUVELOT, Marielle Adrian, Daniel Wipf, Laurent Bonneau, Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, EL Mjiyad, Noureddine, and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, stress biotique, stress abiotique, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, association symbiotique, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, résistance de la vigne, mycorhization, viticulture, écosystème
Abstract

Prod 2017-372 SPE IPM INRA UB; National audience

Published
2017

17. A methodological framework to assess whether biostimulants can impact grapevine physiology and its responsiveness to elicitor-induced resistance

Author

Yuko Krzyzaniak, Marie-Claire Heloir, Estelle Moreau, Sophie TROUVELOT, Marielle Adrian, Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Parc Technopolitain Atalante, and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2017

18. Les biostimulants améliorent-ils la résistance induite par des éliciteurs contre le mildiou de la vigne ?

Author

Yuko Krzyzaniak, Marie-Claire Heloir, Estelle Moreau, Sophie TROUVELOT, Marielle Adrian, Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Parc Technolopitain Atalante, Partenaires INRAE, Université de Bourgogne Franche-Comté (COMUE) (UBFC). FRA., and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2017

19. 'Omics-' for a mapping of grapevine response to elicitors and identification of induced resistance markers

Author

Christelle Guillier, Marie-Claire Heloir, Xavier Daire, Agnès Hovasse, Christine Schaeffer-Reiss, Philippe Schmidt-Kopplin, Marianna Lucio, Chloé Roullier-Gall, Régis Gougeon, Sophie TROUVELOT, Benoît Poinssot, Marielle Adrian, Agroécologie [Dijon], Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté ( UBFC ), Laboratoire de Spectrométrie de Masse BioOrgan, Université de Strasbourg ( UNISTRA ), Centre National de la Recherche Scientifique ( CNRS ), Institut Pluridisciplinaire Hubert Curien ( IPHC ), Research Unit Analytical BioGeoChemistry ( BGC ), Helmholtz-Zentrum München ( HZM ), BGC Research Unit Analytical BioGeoChemistry, Germany Research Center Environmental Health, Analytical BioGeoChemistry, Institut Universitaire de la Vigne et du Vin 'Jules Guyot' ( IUVV Jules Guyot ), Université de Bourgogne ( UB ), ProdInra, Archive Ouverte, Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Université de Strasbourg (UNISTRA), Centre National de la Recherche Scientifique (CNRS), Institut Pluridisciplinaire Hubert Curien (IPHC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Research Unit Analytical BioGeoChemistry (BGC), Helmholtz-Zentrum München (HZM), Institut Universitaire de la Vigne et du Vin 'Jules Guyot' (IUVV Jules Guyot), Université de Bourgogne (UB), Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Germany Research Center for Environmental Health, Analytical BioGeoChemistry, and European Fondation for Plant Pathology (EFPP). INT.

Subjects
[SDV] Life Sciences [q-bio], elicitor, [ SDV ] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], fungi, omics, food and beverages, induced resistance, grapevine
Abstract

SPEIPMINRAUBCNRS; "Omics-" for a mapping of grapevine response to elicitors and identification of induced resistance markers . 12. European Fondation for Plant Pathology (EFPP) & 10. French Society for Plant Pathology Conference ‘Deepen Knwoledge in Plant Pathology for Innovative Agro-Ecology’

Published
2017

20. A combined use of biostimulants and resistance inducers to improve wheat protection

Author

Fourquez, A., Magnin-Robert, M., Randoux, B., Ph. Reignault, Siah, A., Halama, P., Gacoin, M., Gaveau, N., Yuko Krzyzaniak, Sophie TROUVELOT, Marie-Claire Heloir, Marielle Adrian, Agroécologie [Dijon], and Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement

Subjects
[SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017

21. Les biostimulants ont-ils un impact sur la résistance induite par des éliciteurs contre le mildiou de la vigne ?

Author

Yuko Krzyzaniak, Marie-Claire Heloir, Estelle Moreau, Sophie TROUVELOT, Marielle Adrian, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Parc Technopolitain Atalante, Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté ( UBFC ), Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut National de la Recherche Agronomique (INRA). FRA., and ProdInra, Archive Ouverte

Subjects
[SDV] Life Sciences [q-bio], biostimulants, modes d'action, [ SDV ] Life Sciences [q-bio], mildiou, [SDV]Life Sciences [q-bio], éliciteurs, physiologie, vigne
Abstract

SPEINRAUBIPM DOCT; La culture de la vigne constitue un intérêt économique majeur, mais la plupart des cépages sont sensibles aux maladies cryptogamiques telles que le mildiou. Protéger les vignobles contre ces maladies requiert souvent des fongicides de synthèse dont certains posent des problèmes environnementaux et sanitaires. Une stratégie potentielle serait d’utiliser des éliciteurs, molécules capables d’activer les réactions de défenses et d’établir un état de résistance face à une future attaque d’agents pathogènes. Cette stratégie est efficace sur des vignes cultivées en serres, mais reste non maîtrisée en conditions extérieures. Notre objectif est donc d’identifier des solutions susceptibles d’en améliorer l’efficacité afin de maîtriser cette stratégie au vignoble. Contrairement à un fongicide qui agit directement sur l’agent pathogène, l’induction de résistance sollicite la plante et peut être couteuse en énergie. Son efficacité peut donc être conditionnée par l’état physiologique de cette dernière. Or, d’autres composés appelés biostimulants, sont capables quant à eux d’améliorer l’état physiologique de la plante, en renforçant par exemple l’absorption racinaire, ou l’activité photosynthétique. L’hypothèse est donc que le renforcement de l’état physiologique par l’application de biostimulants permettrait d’aider la vigne à mettre en place plus efficacement ses mécanismes de défense, et à terme, d’accroitre la protection assurée par les éliciteurs. Les objectifs de cette thèse sont donc, pour le pathosystème vigne/Plasmopara viticola (agent du mildiou) : 1. Evaluer l’efficacité d’une série d’éliciteurs candidats seuls en protection contre le mildiou et étudier leurs modes d’action ; 2. Définir une méthodologie permettant d’étudier l’effet d’une série de biostimulants candidats, puis évaluer leurs effets seuls sur le développement (phénotypage aérien et racinaire) et le fonctionnement (activité photosynthétique, métabolisme primaire et phytohormones) de la vigne ; 3. Etudier l’effet combiné de biostimulants et d’éliciteurs sur la résistance résultante contre le mildiou.

Published
2017

22. Alterations in Grapevine Leaf Metabolism Occur Prior to Esca Apoplexy Appearance

Author

Marielle Adrian, Christophe Clément, Patricia Letousey, Mourad Harir, Alessandro Spagnolo, Lucile Jacquens, Armelle Vallat, Fanja Rabenoelina, Philippe Schmitt-Kopplin, Chloé Roullier-Gall, Florence Fontaine, Eliane Abou-Mansour, Maryline Magnin-Robert, Sophie Trouvelot, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, German Research Center for Environmental Health - Helmholtz Center München (GmbH), Chair of Analytical Food Chemistry, Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM), Université de Neuchâtel (UNINE), Plant Biology Department, and Cornell University [New York]

Subjects
0106 biological sciences, 0301 basic medicine, Physiology, [SDV]Life Sciences [q-bio], Biology, 01 natural sciences, Mass Spectrometry, Transcriptome, 03 medical and health sciences, chemistry.chemical_compound, Metabolomics, Gene Expression Regulation, Plant, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Vitis, RNA, Messenger, Genetic Association Studies, In Situ Hybridization, Plant Diseases, Plant Proteins, Regulation of gene expression, Fourier Analysis, Gene Expression Profiling, fungi, Fungi, Reproducibility of Results, food and beverages, General Medicine, Metabolism, Glutathione, Cyclotrons, Plant Leaves, Gene expression profiling, 030104 developmental biology, Biochemistry, chemistry, Shoot, [SDE]Environmental Sciences, Metabolome, Phloem, Agronomy and Crop Science, Metabolic Networks and Pathways, 010606 plant biology & botany
Abstract

IPM SPE UB; International audience; Esca disease is one of the major grapevine trunk diseases in Europe and the etiology is complex, since several inhabiting fungi are identified to be associated with this disease. Among the foliar symptom expressions, the apoplectic form may be distinguished and characterized by sudden dieback of shoots, leaf drop, and shriveling of grape clusters in a few days that can ultimately induce the plant death. To further understand this drastic event, we conducted transcriptomic and metabolomic analyses to characterize responses of leaves during the period preceding symptom appearance (20 and 7 days before foliar symptom expression) and at the day of apoplexy ex- pression. Transcriptomic and metabolomic analyses provide signatures for the apoplectic leaves and most changes concerning the metabolism of carbohydrates, amino acids, and phenyl- propanoids. In deciphering glutathione-S-transferase (GST), its preferential location in phloem, correlated with the upregulation of GST genes and a decrease of the glutathione level, offers further support to the putative role of glutathione during apo- plexy expression.

Published
2017
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23. Metabolomic and cytological approaches to better understand grapevine trunk diseaeses disorders

Author

Sophie Trouvelot, Christelle Guillier, Lucile JACQUENS, Antonin Douillet, Samuel Marchat, Claire Grosjean, Chloé Rouiller-Gall, Philippe Schmitt-Kopplin, Philippe Larignon, Florence Fontaine, Marielle Adrian, ProdInra, Migration, Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Research Unit Analytical BioGeoChemistry (BGC), Helmholtz-Zentrum München (HZM), Chair of Analytical Food Chemistry, Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM), Institut Français de la Vigne et du Vin (IFV), Unité de Recherche Vigne et Vins de Champagne Stress et Environnement - EA 4707 (URVVC), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), and Université de Reims Champagne Ardenne (URCA). FRA.

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2017

24. Biocontrol of grapevine aerial and root pathogens by Paenibacillus sp strain B2 and paenimyxin in vitro and in planta

Author

Baodong Chen, Xiaolin Li, Zhipeng Hao, Silvio Gianinazzi, Diederik van Tuinen, Daniel Wipf, Marielle Adrian, Odile Chataignier, Vivienne Gianinazzi-Pearson, Léon Fayolle, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Centre National de la Recherche Scientifique (CNRS), Université Bourgogne Franche-Comté [COMUE] (UBFC), State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences [Changchun Branch] (CAS), AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, College of Resources and Environmental Sciences, China Agricultural University (CAU), Conseil Regional de Bourgogne, France [079201A AO40S3619], National Natural Science Foundation of China [41571250], China Scholarship Council, Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté ( UBFC ), Centre National de la Recherche Scientifique ( CNRS ), Université Bourgogne Franche-Comté ( UBFC ), Chinese Academy of Sciences [Changchun Branch] ( CAS ), and China Agricultural University ( CAU )

Subjects
0106 biological sciences, 0301 basic medicine, [SDV]Life Sciences [q-bio], 01 natural sciences, Xiphinema index, Defence related genes, Microbiology, 03 medical and health sciences, Pathosystem, Paenibacillus, Botany, Plant defense against herbivory, Botrytis cinerea, Oomycete, [ SDV ] Life Sciences [q-bio], biology, fungi, food and beverages, Biocontrol, biology.organism_classification, Paenimyxin, 030104 developmental biology, Insect Science, Plasmopara viticola, Chitinase, biology.protein, Agronomy and Crop Science, Grapevine pathogens, 010606 plant biology & botany
Abstract

Paenibacillus sp. strain B2, isolated from the mycorrhizosphere of Sorghum bicolor , was previously shown to possess antagonistic activity against fungi and bacteria due to the secretion of the peptide paenimyxin. The effects of strain B2 and paenimyxin were assessed against grapevine pathogens as they potentially represent a non-polluting complement or an alternative biocontrol agent to existing disease management strategies. In this study, the effects of Paenibacillus sp. strain B2 and paenimyxin were analyzed in vitro or in planta on the aerial or root pathogens of grapevine Botrytis cinerea , Plasmopara viticola and Xiphinema index . The bacterium significantly reduced B. cinerea development in vitro , and substantially decreased X. index numbers in soil and gall formation in the root system of grapevine plants. In vitro , paenimyxin inhibited the mycelial growth of B. cinerea , the mobility of the zoospores of P. viticola and X . index activity. Supplying paenimyxin at the time of inoculation of leaf tissue with P. viticola decreased infection sites, compared to application prior to fungal inoculation. Using the P. viticola /grapevine pathosystem, we showed that paenimyxin acts as a biopesticide against the oomycete but does not trigger plant defense. Conversely, Paenibacillus sp. strain B2 can induce the expression of the defense genes CHI (chitinase), PR-3 (proteinase inhibitor) and LOX (lipoxygenase). Altogether, the results obtained highlight the interest of Paenibacillus sp. strain B2 and paenimyxin for the biocontrol of different grapevine diseases.

Published
2017
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25. Solutions innovantes pour la santé de la vigne et du blé associant applications de biostimulants et SDP à des technologies d'imagerie et d'agroéquipements

Author

Marie-Claire Heloir, Sophie TROUVELOT, Marielle Adrian, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, ProdInra, Archive Ouverte, Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté ( UBFC ), and Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement

Subjects
[SDV] Life Sciences [q-bio], [ SDV ] Life Sciences [q-bio], [SDV]Life Sciences [q-bio]
Abstract

IPMSPEUB; Solutions innovantes pour la santé de la vigne et du blé associant applications de biostimulants et SDP à des technologies d'imagerie et d'agroéquipements. Journée Recherches Vigne et Vin en Bourgogne

Published
2016

26. Grapevine trunk diseases : a true economical difficulty

Author

Marielle Adrian, Christophe Bertsch, Sophie TROUVELOT, Philippe Larignon, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA)), Institut Français de la Vigne et du Vin (IFV), and Institut Universitaire de la Vigne et du Vin 'Jules Guyot' (IUVV). FRA.

Subjects
[SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2016

27. Identification of reference genes suitable for qRT-PCR in grapevine and application for the study of the expression of genes involved in pterostilbene synthesis

Author

Jani Kelloniemi, David Wendehenne, Benoît Poinssot, Marie-Claire Héloir, Marielle Adrian, Magdalena Gamm, Plante - microbe - environnement : biochimie, biologie cellulaire et écologie (PMEBBCE), and Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)

Subjects
0106 biological sciences, Pterostilbene, [SDV]Life Sciences [q-bio], Statistics as Topic, reference gene, Phenylalanine ammonia-lyase, resveratrol, Genes, Plant, o-methyltransferase, 01 natural sciences, 03 medical and health sciences, chemistry.chemical_compound, Gene Expression Regulation, Plant, Reference genes, Stilbenes, Gene expression, Genetics, Vitis, Molecular Biology, Gene, DNA Primers, 030304 developmental biology, Botrytis cinerea, 2. Zero hunger, Regulation of gene expression, 0303 health sciences, biology, Reverse Transcriptase Polymerase Chain Reaction, Reproducibility of Results, General Medicine, Reference Standards, biology.organism_classification, grapevine, 3. Good health, Biochemistry, chemistry, Plasmopara viticola, plasmopara viticola, Software, 010606 plant biology & botany
Abstract

International audience; The recent publication of the grapevine genome sequence facilitates the use of qRT-PCR to study gene expression changes. For this approach, reference genes are commonly used to normalize data and their stability of expression should be systematically validated. Among grapevine defenses is the production of the antimicrobial stilbenic phytoalexins, notably the highly fungitoxic pterostilbene, which plays a crucial role in grapevine interaction with Plasmopara viticola and Botrytis cinerea. As a resveratrol O-methyltransferase (ROMT) gene involved in pterostilbene synthesis was recently identified, we investigated the accumulation of the corresponding transcripts to those of two other stilbene biosynthesis related genes phenylalanine ammonia lyase (PAL) and stilbene synthase (STS) in response to pathogen infection. Using three computer-based statistical methods and C-t values or LRE method generated values as input data, we have first identified two reference genes (VATP16 and 60SRP) suitable for normalization of qPCR expression data obtained in grapevine leaves and berries infected by P. viticola and B. cinerea, respectively. Next, we have highlighted that the expression of ROMT is induced in P. viticola-infected leaves and also in B. cinerea-infected berries, confirming the involvement of pterostilbene in grapevine defenses.

Published
2011
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28. Application foliaire et biodisponibilité des stimulateurs de défenses des plantes. Spécificités et limites en viticulture

Author

Sophie TROUVELOT, Franck Paris, Yuko Krzyzaniak, Samuel Marchat, Grégory Lecollinet, Christian Gauvrit, Laurent Legentil, Vincent Ferrières, Xavier Daire, Marielle Adrian, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Laboratoires Goëmar S.A.S., Ecole Nationale Supérieure de Chimie de Rennes, Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Ecole Nationale Supérieure de Chimie de Rennes (ENSCR), Goëmar – Parc Technopolitain Atalante, Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), ProdInra, Archive Ouverte, Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), EL Mjiyad, Noureddine, Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Rennes-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES), Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté ( UBFC ), Institut des Sciences Chimiques de Rennes ( ISCR ), Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -Ecole Nationale Supérieure de Chimie de Rennes-Institut National des Sciences Appliquées ( INSA ) -Centre National de la Recherche Scientifique ( CNRS ), Centre National de la Recherche Scientifique ( CNRS ), and Parc Technopolitain Atalante

Subjects
[SDE] Environmental Sciences, [ SDV ] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], éliciteur, hydrophile, stimulateur de défenses des plantes, [SDV] Life Sciences [q-bio], biodisponibilité, sdp, [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, cuticule, vigne, ComputingMilieux_MISCELLANEOUS
Abstract

IPMSPEINRAUBDOCT; Application foliaire et biodisponibilité des stimulateurs de défenses des plantes. Spécificités et limites en viticulture

Published
2016

29. Les stimulateurs de défense des plantes (SDP) en viticulture - Un tour d’horizon sur les principes, les applications et les perspectives

Author

Xavier Daire, Annick Chiltz, Christelle Guillier, Marie-Claire Heloir, Lucile JACQUENS, Agnès Klinguer, Benoît Poinssot, Sophie Trouvelot, Marielle Adrian, Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté ( UBFC ), and ProdInra, Archive Ouverte

Subjects
[SDV] Life Sciences [q-bio], [ SDV ] Life Sciences [q-bio], induction de résistance, [SDV]Life Sciences [q-bio], éliciteur, vigne
Abstract

IPMSPEINRAUBCNRS; Les stimulateurs de défense des plantes (SDP) en viticulture - Un tour d’horizon sur les principes, les applications et les perspectives

Published
2016

30. L'uB au coeur de la viticulture durable

Author

Elsa Fachinetti, Juliette Brey-Xambeu, Régis Gougeon, Florian Humbert, Benjamin Bois, Daniel Wipf, Marielle Adrian, Dominique Delmas, Olivier Mathieu, Maria Nikolantonaki, Christian Coelho, Elias Bou-Maroun, Jérôme Rossignol, Hervé Alexandre, Laurent Gautier, Olivier Jacquet, Clémentine Hugol-Gential, Mathilde Rivière, Aurélie Boudenia, Université de Bourgogne (UB), Conservatoire d’Espaces Naturels de Bourgogne (CEN de Bourgogne), Institut Universitaire de la Vigne et du Vin 'Jules Guyot' (IUVV Jules Guyot), Procédés Alimentaires et Microbiologiques (PAM), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Maison des Sciences de l'Homme de Dijon (MSH Dijon (MSHD)), Université de Bourgogne (UB)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), Biogéosciences [UMR 6282] [Dijon] (BGS), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Lipides - Nutrition - Cancer (U866) (LNC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bourgogne (UB)-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Université Bourgogne Franche-Comté [COMUE] (UBFC), Laboratoire Interdisciplinaire Carnot de Bourgogne (LICB), Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Centre Interlangues - Texte, Image, Langage (TIL), Centre Georges Chevrier. Sociétés & Sensibilités [Dijon - UMR7366] (CGC), Communications, Médiations, Organisations, Savoirs [Dijon] (CIMEOS), Université de Bourgogne (UB)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Accueil et Service Support, Service Commun de Formations Continue et par Alternance (SEFCA), Université de Bourgogne, Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Archive Ouverte

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], viticulture durable
Abstract

SPEIPMUB; Ancrée au cœur d’une région viticole historique, l’université de Bourgogne soutient de multiples projets de recherche viti-vinicoles et s’investit dans la formation des futurs professionnels du secteur. Elle est d’ailleurs la seule université française à disposer d’un domaine expérimental, situé à Marsannay-la-Côte. En activité depuis 1955, il apporte à la fois un support pédagogique aux étudiants de l’institut Jules Guyot, qui y effectuent différentes manipulations en conditions réelles, et répond à des demandes d’expérimentations émanant des viticulteurs et vignerons de la région. Dans ce cadre, il teste de nouveaux produits, matériaux ou micro-organismes susceptibles de mieux protéger les vignes et d’optimiser la vinification, notamment dans une optique de durabilité. Une préoccupation grandissante au sein de la filière viti-vinicole. Une action conjointe des chercheurs et des professionnels Permettre aux professionnels d’accéder à des méthodes et des outils favorables à une viticulture durable passe par une relation étroite entre professionnels du secteur et chercheurs. C’est dans ce but qu’a été créé, en juillet 2015, le pôle Bourgogne Vigne et Vin. Ce groupement d’intérêt public, porté à l'origine par l'université de Bourgogne, le conseil régional de Bourgogne et le Bureau Interprofessionnel des Vins de Bourgogne (BIVB) met en relation les besoins du monde professionnel et les recherches menées à l’uB. Et ce ne sont pas les initiatives qui manquent ! A l’université, une quinzaine de laboratoires sont impliqués dans la thématique Vigne et Vin à différentes échelles. Les recherches menées autour de la viticulture durable rassemblent des équipes issues de spécialités très variées, de la physico-chimie à la climatologie, en passant par les sciences humaines ou la santé.

Published
2016

31. Evidence for foliar uptake of sulfated laminarin into grapevine depending on surfactant use and leaf surface

Author

Yuko Krzyżaniak, Franck Paris, Christian Gauvrit, Lecollinet, G., Vincent Ferrières, Marielle Adrian, Laurent Legentil, Xavier Daire, Sophie Trouvelot, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Ecole Nationale Supérieure de Chimie de Rennes (ENSCR), Laboratoires Goëmar S.A.S., and Association Française de Protection des Plantes (AFPP). FRA.

Subjects
microscopie, induction de résistance, Vitis vinifera, perméabilité cuticulaire, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, polysaccharides, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Abstract

SPE IPM UB CT?; International audience; Certains ß-1,3-glucanes, en particulier la laminarine sulfatée (PS3), sont connus pour être des inducteurs de résistance de la vigne contre l’agent pathogène Plasmopara viticola. Bien que leur efficacité soit incontestable, la protection induite reste variable au vignoble. Pour être perçue par la plante, ils nécessitent de franchir les barrières constitutives, et notamment la cuticule. Ainsi, leur faible efficacité au champ pourrait être liée par une pénétration limitée à travers la cuticule des feuilles, suite à leur pulvérisation. Dans cette étude, nous avons mis au point une méthode pour analyser les comportements de diffusion du PS3 dans les feuilles de Vitis vinifera, à l’aide d’un microscope biphotonique. Nos résultats ont révélé que ce polysaccharide est vraisemblablement capable de pénétrer à travers la cuticule des feuilles de vigne, lorsqu’il est formulé dans un adjuvant adéquat. Par ailleurs, les stomates, les parois anticlines et les trichomes se sont révélés être des zones de diffusion préférentielle pour cette molécule. Il a été intéressant de constater que son taux de pénétration était plus important sur la face abaxiale des feuilles (stomatique), que la face adaxiale (sans stomate). De plus, nous sommes parvenus à lier le taux de pénétration, avec l’efficacité de PS3 en tant qu’inducteur de résistance contre le mildiou.

Published
2015

32. Le matériel végétal en viticulture : un levier d’adaptation face aux évolutions

Author

Sophie TROUVELOT, Marielle Adrian, ProdInra, Migration, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, and Crédit Mutuel. Dijon, FRA.

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Bourgogne, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, viticulture, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2015

33. Sesquiterpene volatile organic compounds (VOCs) are markers of elicitation by sulfated laminarine in grapevine

Author

Jörg-Peter Schnitzler, Sophie Trouvelot, Xavier Daire, Marielle Adrian, Karine Gourrat, J. B. Winkler, Malik Chalal, Frank Jamois, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institute of Biochemical Plant Pathology (BIOP), German Research Center for Environmental Health - Helmholtz Center München (GmbH), Centre des Sciences du Goût et de l'Alimentation (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), and Laboratoires Goëmar S.A.S.

Subjects
[SDV]Life Sciences [q-bio], Defence mechanisms, Plant Science, lcsh:Plant culture, Sesquiterpene, Terpene, chemistry.chemical_compound, Laminarin, PTR-QMS, SPME-GC-MS, downy mildew, methylsalicylate, plant defenses, terpenes, Botany, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Plant defense against herbivory, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, lcsh:SB1-1110, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, Original Research, Ptr-qms, Spme-gc-ms, Downy Mildew, Methylsalicylate, Plant Defenses, Terpenes, food and beverages, Elicitor, Horticulture, chemistry, [SDE]Environmental Sciences, Downy mildew, Methyl salicylate
Abstract

SPE IPM UB CT1; International audience; Inducing resistance in plants by the application of elicitors of defense reactions is an attractive plant protection strategy, particularly for grapevine (Vitis vinifera), which is susceptible to severe fungal diseases. Although induced resistance (IR) can be successful under controlled conditions, in most cases, IR is not sufficiently effective for practical disease control under outdoor conditions. Progress in the application of IR requires a better understanding of grapevine defense mechanisms and the ability to monitor defense markers to identify factors, such as physiological and environmental factors, that can impact IR in the vineyard. Volatile organic compounds (VOCs) are well-known plant defense compounds that have received little or no attention to date in the case of grape-pathogen interactions. This prompted us to investigate whether an elicitor, the sulfated laminarin (PS3), actually induces the production of VOCs in grapevine. An online analysis (proton-transfer-reaction quadrupole mass spectrometry) of VOC emissions in dynamic cuvettes and passive sampling in gas-tight bags with solid-phase microextraction-GC-MS under greenhouse conditions showed that PS3 elicited the emission of VOCs. Some of them, such as (E,E)-α-farnesene, may be good candidates as biomarkers of elicitor-IR, whereas methyl salicylate appears to be a biomarker of downy mildew infection. A negative correlation between VOC emission and disease severity suggests a positive role of VOCs in grape defense against diseases.

Published
2015
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34. Soybean and casein hydrolysates induce grapevine immune responses and resistance against Plasmopara viticola

Author

Suzanne Balacey, Nihed Lachhab, Maurizio Boselli, Simona Marianna Sanzani, Annick Chiltz, Antonio Ippolito, Benoît Poinssot, Marielle Adrian, Università degli Studi di Bari Aldo Moro, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, University of Verona (UNIVR), project 'Thirsty Grapes' - Regione Veneto [2307790], project 'ECO_P4' - Regione Puglia [PON02_00186_2866121], and Conseil Regional de Bourgogne

Subjects
0106 biological sciences, phytoalexins, [SDV]Life Sciences [q-bio], Plant Science, resveratrol, Resveratrol, 01 natural sciences, immune response, induced resistance, Transcriptome, chemistry.chemical_compound, immunité induite, Soybean hydrolysate, Original Research Article, Pathogen, 2. Zero hunger, chemistry.chemical_classification, 0303 health sciences, biology, Phytoalexin, food and beverages, Casein hydrolysate, Plasmopara viticola, [SDE]Environmental Sciences, plant immunity, résistance induite, lcsh:Plant culture, grapevine, Hydrolysate, Microbiology, éliciteur de résistance, 03 medical and health sciences, Immunity, protein hydrolysates, Botany, mildiou de la vigne, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, hydrolysat de protéine, protéine pr, lcsh:SB1-1110, 030304 developmental biology, Vitis vinifera, extrait de soja, gène de défense, hydrolysat de caséine, biology.organism_classification, chemistry, Downy mildew, 010606 plant biology & botany
Abstract

International audience; Plasmopara viticola, the causal agent of grapevine downy mildew, is one of the most devastating grape pathogen in Europe and North America. Although phytochemicals are used to control pathogen infections, the appearance of resistant strains and the concern for possible adverse effects on environment and human health are increasing the search for alternative strategies. In the present investigation, we successfully tested two protein hydrolysates from soybean (soy) and casein (cas) to trigger grapevine resistance against P. viticola. On Vitis vinifera cv. Marselan plants, the application of soy and cas reduced the infected leaf surface by 76 and 63%, as compared to the control, respectively. Since both hydrolysates might trigger the plant immunity, we investigated their ability to elicit grapevine defense responses. On grapevine cell suspensions, a different free cytosolic calcium signature was recorded for each hydrolysate, whereas a similar transient phosphorylation of two MAP kinases of 45 and 49 kDa was observed. These signaling events were followed by transcriptome reprogramming, including the up-regulation of defense genes encoding pathogenesis-related (PR) proteins and the stilbene synthase enzyme responsible for the biosynthesis of resveratrol, the main grapevine phytoalexin. Liquid chromatography analyses confirmed the production of resveratrol and its dimer metabolites, delta- and epsilon-viniferins. Overall, soy effects were more pronounced as compared to the cas ones. Both hydrolysates proved to act as elicitors to enhance grapevine immunity against pathogen attack.

Published
2014
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35. Image analysis methods for assessment of H2O2 production and Plasmopara viticola development in grapevine leaves: application to the evaluation of resistance to downy mildew

Author

Charles Schneider, Marielle Adrian, Marie-Claire Héloir, Xavier Daire, Ian Li Kim Khiook, Benjamin Bois, Sophie Trouvelot, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Biogéosciences [UMR 6282] [Dijon] (BGS), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Institut Universitaire de la Vigne et du Vin 'Jules Guyot' (IUVV Jules Guyot), Université de Bourgogne (UB), Financial support from the BIVB (Bureau Interprofessionnel des Vins de Bourgogne) and the Conseil Régional de Bourgogne., Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Biogéosciences [Dijon] ( BGS ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Institut Universitaire de la Vigne et du Vin 'Jules Guyot' ( IUVV Jules Guyot ), Université de Bourgogne ( UB ), and Agricultural University of Athens (AUA). GRC.

Subjects
0106 biological sciences, Microbiology (medical), Antifungal, medicine.drug_class, [SDV]Life Sciences [q-bio], H2O2, Context (language use), 01 natural sciences, Microbiology, Image analysis, 03 medical and health sciences, Plasmopara viticola, medicine, Plant defense against herbivory, Image Processing, Computer-Assisted, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Vitis, image analysis, downy mildew, grapevine, resistance tests, Molecular Biology, [ SDV.MP.MYC ] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, Analysis method, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, 030304 developmental biology, Disease Resistance, Plant Diseases, 2. Zero hunger, 0303 health sciences, Resistance (ecology), biology, Resistance tests, Reproducibility of Results, food and beverages, Hydrogen Peroxide, biology.organism_classification, Fungicide, Plant Leaves, Horticulture, Agronomy, Oomycetes, [SDE]Environmental Sciences, Downy mildew, Grapevine, 010606 plant biology & botany
Abstract

article i nfo The grapevine downy mildew (Plasmopara viticola) provokes severe damages and destroys the harvest in the absence of an effective protection. Numerous fungicide treatments are thus generally necessary. To promote a sustainable production, alternative strategies of protection including new antifungal molecules, resistant geno- types or elicitor-induced resistance are under trial. To evaluate the relevance of these strategies, resistance tests are required. In this context, three image analysis methods were developed to read the results of tests performed to assessP.viticolasporulation and mycelial development, and H 2 O 2 production in leaves. They have been validated using elicitors of plant defenses. These methods are reliable, innovative, rapid, and their modular concept allows their further adaptation to other host-pathogen systems. © 2013 Elsevier B.V. All rights reserved.

Published
2013
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36. The grapevine flagellin receptor VvFLS2 differentially recognizes flagellin-derived epitopes from the endophytic growth-promoting bacterium Burkholderia phytofirmans and plant pathogenic bacteria

Author

Stéphan Dorey, Marielle Adrian, Christophe Clément, Freddy Boutrot, Cyril Zipfel, Benoît Poinssot, Olivier Fernandez, Xavier Daire, Marie-Claire Héloir, Jani Kelloniemi, Lucie Trdá, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), The Sainsbury Laboratory [Norwich] (TSL), Agence Nationale de la Recherche, Comite National des Interprofessions des Vins d'appelation d'origine (Genoplante 'Safegrape' project) [08-GENO-148G], Conseil Regional de Bourgogne, Bureau Interprofessionnel des Vins de Bourgogne (PARI AGRALE12), Region Champagne-Ardenne, INTERREG IV program France-Wallonie-Vlaanderen (Phytobio project), Gatsby Charitable Foundation, and Biotechnology and Biological Sciences Research Council (BBSRC) [BB/G024936/1]

Subjects
0106 biological sciences, Physiology, Burkholderia phytofirmans, [SDV]Life Sciences [q-bio], flg22, Arabidopsis, Colony Count, Microbial, Plant Science, medicine.disease_cause, 01 natural sciences, Epitopes, Endophytes, Arabidopsis thaliana, Plant Immunity, Vitis, Disease Resistance, Plant Proteins, 0303 health sciences, biology, microbe-associated molecular pattern (MAMP), Xanthomonas campestris, PGPR, [SDE]Environmental Sciences, Botrytis, pattern recognition receptor (PRR), Burkholderia, Molecular Sequence Data, Context (language use), Receptors, Cell Surface, Microbiology, 03 medical and health sciences, Species Specificity, medicine, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Computer Simulation, Amino Acid Sequence, flagellin sensing, 030304 developmental biology, Plant Diseases, fungi, Cell Membrane, Genetic Complementation Test, Pathogenic bacteria, biology.organism_classification, Vitis vinifera, Mutation, biology.protein, Reactive Oxygen Species, Flagellin, Bacteria, 010606 plant biology & botany
Abstract

International audience; The role of flagellin perception in the context of plant beneficial bacteria still remains unclear. Here, we characterized the flagellin sensing system flg22-FLAGELLIN SENSING 2 (FLS2) in grapevine, and analyzed the flagellin perception in the interaction with the endophytic plant growth-promoting rhizobacterium (PGPR) Burkholderia phytofirmans. The functionality of the grapevine FLS2 receptor, VvFLS2, was demonstrated by complementation assays in the Arabidopsis thaliana fls2 mutant, which restored flg22-induced H2O2 production and growth inhibition. Using synthetic flg22 peptides from different bacterial origins, we compared recognition specificities between VvFLS2 and AtFLS2. In grapevine, flg22-triggered immune responses are conserved and led to partial resistance against Botrytis cinerea. Unlike flg22 peptides derived from Pseudomonas aeruginosa or Xanthomonas campestris, flg22 peptide derived from B. phytofirmans triggered only a small oxidative burst, weak and transient defense gene induction and no growth inhibition in grapevine. Although, in Arabidopsis, all the flg22 epitopes exhibited similar biological activities, the expression of VvFLS2 into the fls2 background conferred differential flg22 responses characteristic for grapevine. These results demonstrate that VvFLS2 differentially recognizes flg22 from different bacteria, and suggest that flagellin from the beneficial PGPR B. phytofirmans has evolved to evade this grapevine immune recognition system.

Published
2013
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37. How Plasmopara viticola is able to alter grapevine leaf physiology

Author

Magdalena Gamm, Marie-Claire Héloir, Sophie TROUVELOT, Nathalie Vaillant Gaveau, Gérard Alcaraz, Christophe Clément, Marielle Adrian, ProdInra, Migration, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Unité de recherche Plantes et Systèmes de Culture Horticoles (PSH), Institut National de la Recherche Agronomique (INRA), Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), and Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Université Victor Segalen - Bordeaux 2-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2012

38. Induced resistance in grapevine: from concept to vineyard application

Author

Benoît Poinssot, Lucie Trdá, Jani-Petri Kelloniemi, Emilie Steimetz, Marie-Claire Heloir, Sophie TROUVELOT, Xavier Daire, Marielle Adrian, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, Pattern Recognition Receptors (PRR), plant innate immunity, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, Microbe Associated Molecular Patterns (MAMP)
Abstract

SPE IPM Chapitre 11; International audience

Published
2012

39. Effects of resveratrol on the ultrastructure of Botrytis cinerea conidia and biological significance in plant/pathogen interactions

Author

Philippe Jeandet, Marielle Adrian, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, UNITE DE RECHERCHE VIGNES ET VINS DE CHAMPAGNE - STRESS ET ENVIRONNEMENT - EA2069 (URVV - SE), and Université de Reims Champagne-Ardenne (URCA)

Subjects
0106 biological sciences, [SDV]Life Sciences [q-bio], Resveratrol, 01 natural sciences, Conidium, chemistry.chemical_compound, Botrytis cinerea, Drug Discovery, Stilbenes, DISEASE RESISTANCE, Vitis, Pathogen, chemistry.chemical_classification, ELECTRON-MICROSCOPY, 0303 health sciences, biology, Phytoalexin, food and beverages, Biological activity, General Medicine, Spores, Fungal, VITIS-VINIFERA LEAVES, Antimicrobial, ABC TRANSPORTER BCATRB, 3. Good health, Host-Pathogen Interactions, [SDE]Environmental Sciences, Grapevine, Botrytis, STILBENE PHYTOALEXINS, METABOLISM, Microbiology, 03 medical and health sciences, Phytoalexins, Botany, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, PLANTS, PHYTOALEXIN PHASEOLLIN, Mode of action, 030304 developmental biology, Plant Diseases, Pharmacology, fungi, biology.organism_classification, chemistry, GRAPEVINE LEAVES, CAUSAL AGENT, 010606 plant biology & botany
Abstract

International audience; Many roles have been ascribed to stilbenes, namely as antimicrobial, deterrent or repellent compounds in plants, protecting them from attacks by fungi, bacteria, nematodes or herbivores, acting both as constitutive and active defense (phytoalexin) compounds. More recently, stilbenes (especially resveratrol and its derivatives) were acclaimed for their wondrous effects and wide range of purported healing and preventive powers as cardioprotective, antitumor, neuroprotective and antioxidant agents. Although there is a huge number of works concerning the role of resveratrol in human health, reports on the antifungal activity of this compound are still scarce. This study was thus conducted in order to investigate the toxicity of resveratrol at an ultrastructural level to dormant conidia of Botrytis cinerea, the causal microorganism for gray mold. In grapevine particularly, this disease can affect all the green organs but is particularly damaging for ripening berries. Observations using transmission electron microscopy showed the occurrence of damages on conidia treated with sub-lethal doses, that is, 60 mu g/mL (2.6 x 10(-4) M) of resveratrol, a concentration usually reached in grapevine leaves and grape berries challenged by this pathogen. These results provide further data about the overall mode of action of this phytoalexin and its role in the B. cinerea/grapevine interaction. (C) 2012 Elsevier B.V. All rights reserved.

Published
2012
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40. Influence of leaf age on induced resistance in grapevine against Plasmopara viticola

Author

Katia Gindro, Adeline Bordier, Marielle Adrian, Benoît Poinssot, Xavier Daire, Emilie Steimetz, Sophie Trouvelot, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Agroscope, and Laboratoires Goëmar S.A.S.

Subjects
0106 biological sciences, Zoospore, [SDV]Life Sciences [q-bio], Plant Science, Age-related resistance, 01 natural sciences, induced resistance, 03 medical and health sciences, Laminarin, chemistry.chemical_compound, vitis vinifera, Botany, Genetics, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Colonization, priming, 030304 developmental biology, Oomycete, 0303 health sciences, age-related resistance, biology, Inoculation, food and beverages, biology.organism_classification, chemistry, Plasmopara viticola, [SDE]Environmental Sciences, Downy mildew, plant development, plasmopara viticola, 010606 plant biology & botany
Abstract

International audience; Sulfated laminarin (PS3) has previously been shown to induce resistance of grapevine leaves against the oomycete Plasmopara viticola, the causal agent of grape downy mildew. Here, we observed that the level of PS3-induced resistance (PS3-IR) was higher in the adult leaf (in position P3) than in the younger, not fully expanded leaf (in position P1, located above P3). By investigating grapevine defense reactions upon PS3 treatment and inoculation, we found that the production of H2O2, of phytoalexins, and the deposition of phenolics were more abundant in P3 than in P1 leaves. In addition, PS3 significantly reduced stomatal colonization by zoospores only in P3 leaves. Thus, the capacity of an adult leaf to express a higher level of defense reactions during PS3-IR may partly explain why it exhibits a more elevated resistance when compared to a young leaf, still in growth. These findings have likely practical consequences in induced resistance application

Published
2012
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41. Activation of Grapevine Defense Mechanisms: Theoretical and Applied Approaches

Author

Sophie Trouvelot, Xavier Daire, Benoît Poinssot, Magdalena Gamm, Marielle Adrian, Marie-Claire Héloir, Agroécologie [Dijon], and Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement

Subjects
grapevine, defence réactions, induced résistance, elicitors, 0106 biological sciences, chemistry.chemical_classification, 0303 health sciences, Innate immune system, [SDV]Life Sciences [q-bio], Phytoalexin, Defence mechanisms, food and beverages, Biology, Resveratrol, 01 natural sciences, Cell biology, Elicitor, Cell wall, 03 medical and health sciences, chemistry.chemical_compound, chemistry, [SDE]Environmental Sciences, Plant defense against herbivory, Limited disease, 030304 developmental biology, 010606 plant biology & botany
Abstract

part 4.; International audience; Grapevine, as other plants, possesses an innate immune system that usually prevents infection by pathogens. General elicitors are compounds of different biochemical families capable of inducing plant defense reactions. In grapevine, the cascade of defense events induced by elicitors has been studied among others in cell suspensions. The perception of the elicitor triggers signaling events that allow the activation of defense genes encoding PR proteins and other proteins involved in phytoalexin production and cell wall reinforcement. The grapevine phytoalexins resveratrol and derivated compounds have been largely studied. In addition to their antimicrobial activity, they may also contribute to cell wall reinforcement. The mode of action and activity of elicitors depends on their chemical structure. Elicitors are of particular interest for crop protection since they can not only elicit defenses in a broad spectrum of plants, but are also mostly deprived of toxicity and suitable for industrial production from abundant sources. In spite of promising results, on the whole, application of induced resistance in the vineyard still often suffers from inconsistency and provides only limited disease control up to now.

Published
2011
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42. Laminarin elicits defense responses in grapevine and induces protection against botrytis cinerea and plasmopara viticola

Author

Aziz Aziz, Bernard Lambert, Annie Bézier, Alain Pugin, Jean-Marie Joubert, Xavier Daire, Marielle Adrian, Benoît Poinssot, UNITE DE RECHERCHE VIGNES ET VINS DE CHAMPAGNE - STRESS ET ENVIRONNEMENT - EA2069 (URVV - SE), Université de Reims Champagne-Ardenne (URCA), Plante - microbe - environnement : biochimie, biologie cellulaire et écologie (PMEBBCE), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD), Laboratoires Goëmar S.A.S., Aziz, Aziz, Poinssot, Benoît, and Pugin, Alain

Subjects
0106 biological sciences, Glycoside Hydrolases, Physiology, [SDV]Life Sciences [q-bio], grapevine, botrytis cinerea, plasmopara viticola, induce protection, 01 natural sciences, laminarine, 03 medical and health sciences, Laminarin, chemistry.chemical_compound, Gene Expression Regulation, Plant, Polysaccharides, Phytoalexins, Botany, Vitis, Phycomycetes, Vitis vinifera, Glucans, 030304 developmental biology, Botrytis cinerea, DNA Primers, 0303 health sciences, Uncinula necator, botrytis de la vigne, biology, Base Sequence, Cell Death, Plant Extracts, Terpenes, Chitinases, fungi, food and beverages, General Medicine, Fungi imperfecti, biology.organism_classification, Eutypa lata, chemistry, Oomycetes, Plasmopara viticola, Botrytis, vigne cultivée, Agronomy and Crop Science, Sesquiterpenes, 010606 plant biology & botany, Signal Transduction
Abstract

International audience; Grapevine (Vittis vinifera L.) is susceptible to many pathogens, such as Botrytis cinerea, Plasmopara viticola, Uncinula necator, and Eutypa lata. Phytochemicals are used intensively in vineyards to limit pathogen infections, but the appearance of pesticide-resistant pathogen strains and a desire to protect the environment require that alternative strategies be found. In the present study, the, beta-1,3-glucan laminarin derived from the brown algae Laminaria digitata was shown both to be an efficient elicitor of defense responses in grapevine cells and plants and to effectively reduce B. cinerea and P viticola development on infected grapevine plants. Defense reactions elicited by laminarin in grapevine cells include calcium influx, alkalinization of the extracellular medium, an oxidative burst, activation of two mitogen-activated protein kinases, expression of 10 defense-related genes with, different kinetics and intensities, increases in chitinase and beta-1,3-glucanase activities, and the production of two phytoalexins (resveratrol and epsilon-viniferin). Several of these effects were checked and confirmed in whole plants. Laminarin did not induce cell death. When applied to grapevine plants, laminarin reduced infection by B. cinerea and R viticola by approximately 55 and 75%, respectively. Our data describing a large set of defense reactions in grapevine indicate that the activation of defense responses using elicitors could be a valuable strategy to protect plants against pathogens.

Published
2003
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43. Associer biostimulants, SDP, systèmes d’imagerie et de pulvérisation au service de la santé du vignoble

Author

Yuko Krzyzaniak, Olivier Brousse, Frédéric Cointault, Marie-Claire Heloir, Estelle Moreau, Yvan Picaud, Christophe Salon, Jean-Claude Simon, Corinne Trarieux, Sophie TROUVELOT, Nathalie Vaillant-Gaveau, Marielle Adrian, ProdInra, Archive Ouverte, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Centre National de la Recherche Scientifique (CNRS), Goëmar-Arysta LifeScience, Orange Labs, Bureau Interprofessionnel des Vins de Bourgogne (BIVB), Unité de Recherche Vigne et Vins de Champagne Stress et Environnement - EA 4707 (URVVC), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté ( UBFC ), Centre National de la Recherche Scientifique ( CNRS ), Bureau Interprofessionnel des Vins de Bourgogne ( BIVB ), Unité de Recherche Vigne et Vins de Champagne Stress et Environnement - EA 4707 ( URVVC ), Université de Reims Champagne-Ardenne ( URCA ) -SFR Condorcet, and Université de Reims Champagne-Ardenne ( URCA ) -Université de Picardie Jules Verne ( UPJV ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Reims Champagne-Ardenne ( URCA ) -Université de Picardie Jules Verne ( UPJV ) -Centre National de la Recherche Scientifique ( CNRS )

Subjects
[SDV] Life Sciences [q-bio], SDP, [ SDV ] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], vigne, biostimulant
Abstract

Prod?IPMGEAPSISPEEAINRAUBAGROSUPDOCT; Associer biostimulants, SDP, systèmes d’imagerie et de pulvérisation au service de la santé du vignoble

44. Membres du comité d’organisation

Author

Philippe Larignon, Marielle Adrian, Pierre-Emmanuel Courty, Florian Humbert, Sophie TROUVELOT, Claire Grosgean, Daniel Wipf, Jean-Louis Lacroix, Institut Français de la Vigne et du Vin (IFV), Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut Universitaire de la Vigne et du Vin 'Jules Guyot' (IUVV Jules Guyot), Université de Bourgogne (UB), Chambre d’Agriculture de Bourgogne Franche-Comté, and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

45. Mycorhization et viticulture : État des lieux et services écosystémiques rendus au vignoble

Author

Alice Drain, Diederik van Tuinen, Eric Bernaud, Sophie TROUVELOT, Marielle Adrian, Daniel Wipf, Laurent Bonneau, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, ProdInra, Migration, and EL Mjiyad, Noureddine

Subjects
[SDE] Environmental Sciences, vignoble, mycorhization, viticulture, [SDV]Life Sciences [q-bio], champignon, écosystème, biodiversité, nutrition minérale, [SDV] Life Sciences [q-bio], stress, [SDE]Environmental Sciences, association symbiotique, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, biomasse, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, agriculture raisonnée, agent pathogène, sécheresse, salinité
Abstract

Prod 2017-371 SPE IPM INRA UB; National audience

46. Biostimulation of grapevine : mode of action and possible agronomic uses

Author

Yuko Krzyżaniak, Sophie TROUVELOT, Marie-Claire Héloir, Fourquez, P., Jean-Bernard Magnin-Robert, Randoux, B., Siah, A., Patrice Halama, Emmanuelle Moreau, Marielle Adrian, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV), Université du Littoral Côte d'Opale (ULCO), Biotechnologie et Gestion des Agents Pathogènes en agriculture (BioGAP), Institut Supérieur d'Agriculture de Lille (ISA)-Institut Charles Viollette (ICV) - EA 7394 (ICV), Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de la Recherche Agronomique (INRA)-Université d'Artois (UA)-Institut Supérieur d'Agriculture-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de la Recherche Agronomique (INRA)-Université d'Artois (UA)-Institut Supérieur d'Agriculture, Laboratoires Goëmar S.A.S., Institut National de la Recherche Agronomique (INRA). FRA., Université d'Artois (UA)-Institut National de la Recherche Agronomique (INRA)-Université du Littoral Côte d'Opale (ULCO)-Institut Supérieur d'Agriculture-Université de Lille-Université d'Artois (UA)-Institut National de la Recherche Agronomique (INRA)-Université du Littoral Côte d'Opale (ULCO)-Institut Supérieur d'Agriculture-Université de Lille, Institut Supérieur d'Agriculture de Lille (ISA), and Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Institut Charles Viollette (ICV) - EA 7394 (ICV)

Subjects
biostimulants, elicitors, [SDV]Life Sciences [q-bio], physiology, [SDE]Environmental Sciences, fungi, grapevine, induced resistance, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, food and beverages
Abstract

SPE IPM UB INRA; National audience; Although there is a growing interest for the use of biostimulants in agriculture, only few methods allowing a precise description of their effects on plants have been reported. In the IRIS+ FUI project, two major and highly different worldwide crops, wheat (annual, monocotyledon) and grapevine (perennial, broadleaf), were chosen to deepen our knowledge of such compounds and explore their potential additional interest. The first objective is to develop in greenhouse conditions, a panel of tools and methods to study the impact of a series of biostimulants on the development (aerial and root system biomass measurements and corresponding phenotyping), and the physiology (photosynthetic activity, primary and secondary metabolites) of both plants. The second objective is to check if biostimulants, via their effects on the plant physiology, could be associated to resistance inducer-based control strategies against fungal aerial diseases. Unlike fungicides which directly target path! ogens, resistance inducers request plant metabolism dedicated to defense, a fitnesscostly process. Hence, an improvement of both crops' physiological status by biostimulation is expected to increase its responsiveness to resistance inducer application.

47. Oligosaccharide-triggered plant immunity and plant protection

Author

Alain Pugin, Benoît Poinssot, Sophie TROUVELOT, Marielle Adrian, Xavier Daire, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

48. Membre du comité d'organisation

Author

Philippe Larignon, Marielle Adrian, Pierre-Emmanuel Courty, Florian Humbert, Sophie TROUVELOT, Claire Grosjean, Daniel Wipf, Christelle Guillier, Institut Français de la Vigne et du Vin (IFV), Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut Universitaire de la Vigne et du Vin 'Jules Guyot' (IUVV Jules Guyot), Université de Bourgogne (UB), Chambre d’Agriculture de Bourgogne Franche-Comté, and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

49. Principaux effets de l’arsénite de sodium sur la physiologie de la vigne

Author

Julie Vallet, Guillaume Robert-Siegwald, Christelle Guillier, Marielle Adrian, Sophie TROUVELOT, Lucile Jacquens, Aurélie Songy, Christophe Clément, Philippe Larignon, Marc-Henri Lebrun, Florence Fontaine, SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), BIOlogie et GEstion des Risques en agriculture (BIOGER), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut Français de la Vigne et du Vin (IFV), Institut Français de la Vigne et du Vin (IFV). FRA., and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology
Abstract

National audience; Dans le cadre du projet CASDAR V1301, l’impact d’un traitement à l’arsénite de sodium sur la physiologie de la vigne a été étudié chez le Chardonnay. Deux approches complémentaires, transcriptomique et métabolomique, ont été réalisées sur différents organes (tiges herbacées et tronc en distinguant le bois sain et la zone d’interaction bois sain / bois altéré) de ceps non traités sans symptômes foliaires (témoins), non traités avec symptômes foliaires et traités (n’exprimant donc plus les symptômes et qualifiés de « guéris »). Les échantillons ont été prélevés 6 mois après le traitement (avant vendanges). Parmi les principaux résultats, l’analyse transcriptomique montre que les tiges herbacées présentent des différences d'expression des gènes plus importantes que le bois. Ainsi, les ceps témoins et malades ont des profils d'expression spécifiques et le traitement arsénite conduit lui aussi à un profil d'expression particulier. Parmi ces gènes, nous avons pu identifier un groupe ayant une réponse de type « stigmate de l'infection », car ils sont différentiellement exprimés chez les ceps malades par rapport aux ceps témoins et de façon similaire chez les ceps malades et les ceps « guéris» par le traitement arsénite. Un autre groupe de gènes présente une réponse de type « guérison», car ils sont différentiellement exprimés chez les ceps malades par rapport aux ceps témoins, et avec le même profil d’expression chez les ceps témoins et les ceps ayant été traités à l’arsénite. Ces expériences montrent que l’infection du bois de la vigne a un impact important sur la physiologie des parties aériennes et qu’il est ainsi possible d’identifier des gènes marqueurs de l’infection au niveau de la tige herbacée. De plus, le traitement à l’arsénite a un effet important sur le transcriptome de la vigne et certains gènes pourraient être considérés comme des marqueurs de la « guérison » de la vigne. En complément, l’analyse métabolomique nous a permis de mettre en évidence des signatures métaboliques spécifiques du traitement arsénite tant au niveau des tiges herbacées que des zones ligneuses (zones d'interaction bois sain altéré). Le traitement arsénite impacte donc aussi le métabolome de la vigne et de potentiels métabolites marqueurs de « guérison » sont en cours d’identification. La détermination d’indicateurs de « guérison », gènes et ou métabolites, permettrait de tester plus facilement l’efficacité de futures stratégies de protection contre l’esca. Enfin l’impact du traitement arsénite s'est également confirmé à l'échelle cellulaire (histologie) où des réponses structurales et défensives sont encore observées, 7 mois post-traitement, dans les différents compartiments de la plante.

50. Induced resistance as a strategy for vineyard protection

Author

Emilie Steimetz, Abdelwahad Echairi, Sophie TROUVELOT, Benoît Poinssot, Annick Chiltz, Christelle Guillier, Eric Bernaud, Agnès Klinguer, Marie-Claire Héloir, Marielle Adrian, Xavier Daire, ProdInra, Migration, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, New Ag International., and Réseau Vigne et Vins Septentrional (RVVS). FRA.

Subjects
[SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], fungi, food and beverages, defense elicitor, induced resistance, [SDV] Life Sciences [q-bio], Vitis vinifera, [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Grapevine, plant triggered immunity, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience; As most grown grapevine Vitis vinifera varieties are susceptible to diseases such as downy and powdery mildews, numerous treatments are required to ensure a satisfactory yield and harvest quality. However, the use of phytochemical fungicides has serious drawbacks: some of them are potentially harmful for the environment and human health and contribute to the selection of resistant pathogen strains. Nowadays, in an objective of sustainable viticulture, there is increasing societal request, political incitation and winegrower’s awareness to reduce the use of pesticides. For these reasons, alternative strategies of protection are under research. In our laboratory, we are studying one alternative consisting in the activation of the grapevine defense reactions by compounds called elicitors. Plants are almost constantly in contact with potentially pathogen microorganisms such as oomycetes, fungi or bacteria. However, due to defense mechanisms, disease is finally an exceptional outcome in plant-pathogen interactions. General elicitors are compounds of different biochemical families capable of inducing plant defense reactions. Their perception by the plant triggers signaling events that allow the activation of defense genes encoding PR proteins and other proteins involved in phytoalexin production and cell wall reinforcement. Elicitors possess a potentially high interest for crop protection since they can not only elicit defenses in a broad spectrum of plants, but are also mostly deprived of toxicity and suitable for industrial production from abundant sources. Such a strategy could therefore be included in an integrated pest management or biological control strategy. We propose to sum up the main axis of our research about elicitors and the identification of the key mechanisms involved in induced resistance against downy mildew.

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