1. A variant of LEAFY reveals its capacity to stimulate meristem development by inducingRAX1
- Author
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Vojislava Grbic, Sophie Périgon, Marie Monniaux, Nela Mihajlovic, Gilles Vachon, François Parcy, Gabrielle Tichtinsky, Edwige Moyroud, Robin Michard, Marie Le Masson, Emmanuel Thévenon, Anna Kalinina, Camille Sayou, Hicham Chahtane, Laboratoire de physiologie cellulaire végétale (LPCV), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Recherche Agronomique (INRA)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), Department of Biology, University of Western Ontario, University of Western Ontario (UWO), Charmful ANR (Agence Nationale de la Recherche) program, Natural Sciences and Engineering Research Council of Canada, Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)
- Subjects
0106 biological sciences ,Mutant ,Arabidopsis ,plant ,Plant Science ,01 natural sciences ,LFY ,Gene Expression Regulation, Plant ,Arabidopsis thaliana ,transcription factor ,chemistry.chemical_classification ,0303 health sciences ,Crystallography ,Gene Expression Regulation, Developmental ,food and beverages ,RAX1 ,Plants, Genetically Modified ,flower ,Cell biology ,DNA-Binding Proteins ,Inflorescence ,Meristem ,Flowers ,Biology ,Models, Biological ,03 medical and health sciences ,Auxin ,Two-Hybrid System Techniques ,Axillary bud ,Botany ,meristem development ,Genetics ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Nucleotide Motifs ,Leafy ,Transcription factor ,Alleles ,030304 developmental biology ,angiospermeae ,Arabidopsis Proteins ,fungi ,Cell Biology ,biology.organism_classification ,Protein Structure, Tertiary ,LEAFY ,Plant Leaves ,chemistry ,Seedlings ,Mutation ,gene expression ,Protein Multimerization ,auxin ,Transcription Factors ,010606 plant biology & botany - Abstract
International audience; In indeterminate inflorescences, floral meristems develop on the flanks of the shoot apical meristem, at positions determined by auxin maxima. The floral identity of these meristems is conferred by a handful of genes called floral meristem identity genes, among which the LEAFY (LFY) transcription factor plays a prominent role. However, the molecular mechanism controlling the early emergence of floral meristems remains unknown. A body of evidence indicates that LFY may contribute to this developmental shift, but a direct effect of LFY on meristem emergence has not been demonstrated. We have generated a LFY allele with reduced floral function and revealed its ability to stimulate axillary meristem growth. This role is barely detectable in the lfy single mutant but becomes obvious in several double mutant backgrounds and plants ectopically expressing LFY. We show that this role requires the ability of LFY to bind DNA, and is mediated by direct induction of REGULATOR OF AXILLARY MERISTEMS1 (RAX1) by LFY. We propose that this function unifies the diverse roles described for LFY in multiple angiosperm species, ranging from monocot inflorescence identity to legume leaf development, and that it probably pre-dates the origin of angiosperms.
- Published
- 2013