1. Angiogenic Aptamer-Modified Tetrahedral Framework Nucleic Acid Promotes Angiogenesis In Vitro and In Vivo
- Author
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Shuanglin Peng, Dexuan Xiao, Mengting Liu, Dan Zhao, Yunfeng Lin, Qing He, Yue Sun, Jiajie Li, and Qirong Li
- Subjects
Materials science ,Angiogenesis ,Aptamer ,Mice, Nude ,Neovascularization, Physiologic ,02 engineering and technology ,Mice ,03 medical and health sciences ,Tissue engineering ,Cell Movement ,In vivo ,Nucleic Acids ,Human Umbilical Vein Endothelial Cells ,Animals ,Humans ,General Materials Science ,Cells, Cultured ,Cell Proliferation ,030304 developmental biology ,0303 health sciences ,Tissue Engineering ,Aptamers, Nucleotide ,021001 nanoscience & nanotechnology ,In vitro ,Nanostructures ,Cell biology ,Transplantation ,Endothelial stem cell ,Nucleic acid ,Angiogenesis Inducing Agents ,0210 nano-technology - Abstract
In a search for a solution to large-area soft and hard tissue defects, whether or not tissue regeneration or tissue-substitutes transplantation is used, the problems with angiogenesis need to be solved urgently. Thus, a new and efficient proangiogenic approach is needed. Nanoengineering systems have been considered one of the most promising approaches. In this study, we modify the tetrahedral framework nucleic acid (tFNA) for the first time with two different angiogenic DNA aptamers to form aptamer-tFNA nanostructures, tFNA-Apt02 and tFNA-AptVEGF, and the effects of them on angiogenesis both in vitro and in vivo are investigated. We develop new nanomaterials for enhancing angiogenesis to solve the problem of tissue engineering vascularization and ischemic diseases. The results of our study confirm that tFNA-Apt02 and tFNA-AptVEGF has a stronger ability to accelerate endothelial cell proliferation and migration, tubule formation, spheroid sprouting, and angiogenesis in vivo. We first demonstrate that the engineered novel tFNA-Apt02 and tFNA-AptVEGF have promoting effects on angiogenesis both in vitro and in vivo and provide a theoretical basis and opportunity for their application in tissues engineering vascularization and ischemic diseases.
- Published
- 2021
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