1. Fungicidal and anti-biofilm activities of trimethylchitosan-stabilized silver nanoparticles against Candida species in zebrafish embryos
- Author
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Xin-an Chen, Chih-Hua Lee, Shao-Hung Wang, Jenn-jong Young, Yi-Chuan Cheng, Jang-Jih Lu, Cheng-Cheung Chen, and Tein-Yao Chang
- Subjects
Antifungal Agents ,Embryo, Nonmammalian ,Silver ,Metal Nanoparticles ,02 engineering and technology ,Microbial Sensitivity Tests ,Biochemistry ,Silver nanoparticle ,Microbiology ,Cell Line ,Candida tropicalis ,03 medical and health sciences ,Minimum inhibitory concentration ,Mice ,Structural Biology ,Animals ,Cytotoxicity ,Candida albicans ,Molecular Biology ,Zebrafish ,030304 developmental biology ,Candida ,Ovum ,0303 health sciences ,Chitosan ,biology ,Candida glabrata ,Cell Death ,Chemistry ,Biofilm ,General Medicine ,021001 nanoscience & nanotechnology ,biology.organism_classification ,Corpus albicans ,Biofilms ,0210 nano-technology - Abstract
Herein, positively surface-charged silver nanoparticles (AgNPs) capped with trimethylchitosan nitrate (TMCN) were synthesized using an environmentally friendly method. Nano-sized TMCN-AgNPs (~80 nm) with high zeta potential (>30 mV) provide sufficient static repulsion to stabilize colloid AgNPs in aqueous solutions without aggregation for >3 months. In in vitro cell cycle assays, TMCN-AgNPs showed low cytotoxicity towards L929 cells. A microdilution inhibition assay demonstrated the antifungal potential of TMCN-AgNPs, with a minimum inhibitory concentration of 0.06 mM against Candida tropicalis ATCC 750, and 0.46 mM against both Candida albicans ATCC 76615 and Candida glabrata ATCC 15545. Moreover, the addition of TMCN-AgNPs at 0.23 mM significantly reduced biofilm formation in 96-well plates with C. albicans and C. tropicalis. Importantly, when zebrafish eggs were infected with Candida cells, 0.23 mM TMCN-AgNPs greatly diminished the amount of biofilm on eggs and rescued the survival of embryos by up to 70%.
- Published
- 2019