1. Targeted exhaled breath analysis for detection of Pseudomonas aeruginosa in cystic fibrosis patients
- Author
-
Lieuwe D. J. Bos, Eric G. Haarman, Renate Kos, Els J.M. Weersink, Paul Brinkman, Anke H. Maitland-van der Zee, Aletta D. Kraneveld, Anne H. Neerincx, Harry G.M. Heijerman, Jane C. Davies, Peter J. Sterk, Ariana Lammers, Hettie M. Janssens, Marije G Gerritsen, Tamara Paff, Christof J. Majoor, Pediatric surgery, Pulmonary medicine, Amsterdam Reproduction & Development (AR&D), Cystic Fibrosis Trust, Pediatrics, Pulmonology, General Paediatrics, Graduate School, AII - Inflammatory diseases, AII - Amsterdam institute for Infection and Immunity, CCA -Cancer Center Amsterdam, APH - Personalized Medicine, CCA - Cancer biology and immunology, Intensive Care Medicine, ACS - Heart failure & arrhythmias, and Paediatric Pulmonology
- Subjects
0301 basic medicine ,Pulmonary and Respiratory Medicine ,Adult ,Male ,medicine.medical_specialty ,Pathogen detection ,Adolescent ,Respiratory System ,Amsterdam Mucociliary Clearance Disease (AMCD) Research Group and the Amsterdam UMC Breath Research Group ,medicine.disease_cause ,Cystic fibrosis ,Gastroenterology ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Internal medicine ,medicine ,Humans ,Pseudomonas Infections ,Longitudinal Studies ,Exhaled biomarkers ,Volatile Organic Compounds ,business.industry ,Pseudomonas aeruginosa ,VOC ,Respiratory pathogen ,1103 Clinical Sciences ,medicine.disease ,030104 developmental biology ,Cross-Sectional Studies ,030228 respiratory system ,Breath Tests ,Breath gas analysis ,Exhalation ,Pediatrics, Perinatology and Child Health ,Sputum ,Female ,medicine.symptom ,business - Abstract
Background: Pseudomonas aeruginosa (PA) is an important respiratory pathogen for cystic fibrosis (CF) patients. Routine microbiology surveillance is time-consuming, and is best performed on expectorated sputum. As alternative, volatile organic compounds (VOCs) may be indicative of PA colonisation. In this study, we aimed to identify VOCs associated with PA in literature and perform targeted exhaled breath analysis to recognize PA positive CF patients non-invasively. Methods: This study consisted of 1) a literature review to select VOCs of interest, and 2) a cross-sectional CF study. Definitions used: A) PA positive, PA culture at visit/chronically; B) PA free, no PA culture in ≥12 months. Exhaled VOCs were identified via quadrupole MS. The primary endpoint was the area under the receiver operating characteristics curve (AUROCC) of individual VOCs as well as combined VOCs against PA culture. Results: 241 VOCs were identified in literature, of which 56 were further evaluated, and 13 could be detected in exhaled breath in our cohort. Exhaled breath of 25 pediatric and 28 adult CF patients, PA positive (n=16) and free (n=28) was available. 3/13 VOCs were significantly (p
- Published
- 2021