11 results on '"Jana Petrová"'
Search Results
2. (Atrial fibrallation in a cardiological practice - focused on amiodarone)
- Author
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Radim Kryza, Jana Petrová, and Karel Dvořák
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03 medical and health sciences ,0302 clinical medicine ,030212 general & internal medicine ,030204 cardiovascular system & hematology ,Cardiology and Cardiovascular Medicine - Abstract
Autoři uvaději sve vlastni zkusenosti s peroralnim podavanim amiodaronu u pacientů trpicich fibrilaci sini. Uvaději strucný přehled metabolismu amiodaronu, doporuceneho davkovani a nežadoucich ucinků. Při podavani nizkých davek a peclivem sledovani považuji amiodaron za bezpecne a vysoce ucinne antiarytmikum. © 2019, CKS.
- Published
- 2019
3. The Impact of Growth-Promoting Streptomycetes Isolated from Rhizosphere and Bulk Soil on Oilseed Rape (Brassica napus L.) Growth Parameters
- Author
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Ladislav Ducsay, Jana Maková, Jana Petrová, Juraj Medo, Soňa Javoreková, Nikola Lipková, and Renata Cinkocki
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Siderophore ,oilseed rape ,Geography, Planning and Development ,Bulk soil ,Brassica ,TJ807-830 ,Management, Monitoring, Policy and Law ,TD194-195 ,Streptomyces ,Renewable energy sources ,03 medical and health sciences ,GE1-350 ,030304 developmental biology ,0303 health sciences ,Rhizosphere ,biology ,Environmental effects of industries and plants ,030306 microbiology ,Renewable Energy, Sustainability and the Environment ,Inoculation ,fungi ,food and beverages ,biology.organism_classification ,Environmental sciences ,Horticulture ,Seedling ,PGPS ,Shoot - Abstract
Inoculation of Streptomyces to improve oilseed rape (Brassica napus L.) yields and minimise the use of chemical fertilisers is a promising sustainable strategy. In this study, we isolated 72 actinobacterial strains from rhizosphere of oilseed rape and maize and from bulk soil for screening and characterising their antimicrobial activity. Nine promising strains, identified as Streptomyces sp. by morphology, physiological characteristics, and 16S rRNA gene sequencing, were selected for their plant growth-promoting traits and in planta experiments. The actinobacterial strains were positive for IAA production, siderophore production, and HCN production. In planta experiments were conducted by soaking the oilseed rape seeds in the actinobacterial suspension, followed by plant growth under controlled conditions in a cultivate chamber (22–28 °C, 8 h dark/16 h light, constant humidity 80%). We recorded root and shoot length (cm) and seedling fresh weight (g). For most of the abovementioned parameters, a significant enhancement was observed with strain KmiRC20A118 treatment. The length of the root increased by 53.14%, the shoot length increased by 65.6%, and the weight of the fresh plant by 60% compared to the control. The integrated application of PGPS (Plant Growth Promoting Streptomyces) from the rhizosphere of oilseed rape is a promising strategy to improve the growth of oilseed rape.
- Published
- 2021
4. Reperfusion after pulmonary embolism - long-term follow-up, risk factors, clinical impact
- Author
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Pavel Jansa, Jana Vaclavkova, Vladimir Janovsky, Jana Petrová, Jan Mrózek, and Luboš Kraus
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Adult ,Male ,medicine.medical_specialty ,pulmonary embolism ,Computed Tomography Angiography ,Perfusion Imaging ,Deep vein ,lcsh:Medicine ,030204 cardiovascular system & hematology ,Multimodal Imaging ,General Biochemistry, Genetics and Molecular Biology ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,Internal medicine ,medicine ,Humans ,Thrombolytic Therapy ,Prospective Studies ,Risk factor ,Prospective cohort study ,Aged ,First episode ,business.industry ,lcsh:R ,Anticoagulants ,Middle Aged ,Tricuspid insufficiency ,medicine.disease ,Thrombosis ,Pulmonary hypertension ,Pulmonary embolism ,medicine.anatomical_structure ,030228 respiratory system ,risk factor ,Echocardiography ,incomplete reperfusion ,Reperfusion ,Cardiology ,Female ,business ,Follow-Up Studies - Abstract
Background and Aim. Thromboembolic disease is the third most common cardiovascular disorder and deep vein thrombosis carries the risk of pulmonary embolism (PE). Questions related to reperfusion after PE remain, especially risk factors. Incomplete reperfusion after PE is closely related to the development of chronic thromboembolic pulmonary hypertension. The aim of this study was to determine the relation between reperfusion after PE in the long term over a period of 24 months, laboratory results and clinical risk factors found during the initial PE event. Patients and Methods: 85 consecutive patients with a first episode of acute PE, diagnosed at 4 cardiology clinics, were followed up using clinical evaluation, scintigraphy and echocardiography (6, 12 and 24 months after the PE. 35 patients were in the low risk category (41%), 42 (49%) in the intermediate risk group and 8 (9%) in the high risk category. Results: Perfusion defects persisted in 20 patients (26%) after 6 months, in 19 patients (25%) after 12 months and in 14 patients (19%) after 24 months. The incidence was more frequent in older patients, with more serious (higher risk) PE, increased right ventricular internal diameter during the initial episode, and more significant tricuspid insufficiency in the initial echocardiography. Notably, higher hemoglobin levels were also shown as a significant risk factor. The presence of perfusion defects after 24 months correlated with a concurrent higher pulmonary pressure but not with either patient function or adverse events (recurrence of PE, re-hospitalization or bleeding). In 3 cases (4% of patients), long-term echocardiographic evidence of pulmonary hypertension was detected. Conclusion: Even after 24 months from acute PE with adequate anticoagulation treatment, incomplete reperfusion was found in 19% of patients with a corresponding risk of chronic thromboembolic pulmonary disease and hypertension.
- Published
- 2018
5. Bioactive fractions from cantabrian anchovy (Engraulis encrarischolus) viscera
- Author
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Marina Martínez, Jana Petrová, Ema Carina Rosas-Burgos, Frutos C. Marhuenda, Armando Burgos-Hernández, Paloma Nallely Nuncio-Jauregui, Miroslava Kačániová, Angel Antonio Carbonell-Barrachina, Universidad de Alicante. Departamento de Agroquímica y Bioquímica, and Grupo de Fotoquímica y Electroquímica de Semiconductores (GFES)
- Subjects
0301 basic medicine ,antioxidant ,Antioxidant ,DPPH ,fungistatic ,medicine.medical_treatment ,Ethyl acetate ,lcsh:TX341-641 ,Fractionation ,Ames test ,03 medical and health sciences ,chemistry.chemical_compound ,antibiotic ,lcsh:Technology (General) ,medicine ,Organic chemistry ,anchovy gut ,antimutagenicity ,Chromatography ,ABTS ,Butanol ,Bioquímica y Biología Molecular ,030104 developmental biology ,chemistry ,lcsh:T1-995 ,Antibacterial activity ,lcsh:Nutrition. Foods and food supply ,Food Science ,Biotechnology - Abstract
The potential of cantabrian anchovy (Engraulis encrarischolus) viscera as a source of bioactive compounds is of interest for both, pharmaceutical and food industries. Cantabrian anchovy guts and heads were freeze-dried, extracted with methanol and subjected to fractionation by solvent partitioning using hexane, ethyl acetate, and butanol. Fractions were tested for antimutagenic, antioxidant, antifungal, and antibacterial activity using the Ames test; DPPH, ABTS, and FRAP assays; the radial grown inhibition assay; and the microbroth dilution method, respectively. Five fractions were obtained from the anchovy gut methanolic extract, in addition to the hexane- (HF), ethyl acetate- (EAF), and butanol-soluble (BF) fractions, an aqueous-soluble fraction (ALF) and precipitated crystals (ACF) in this were also obtained. HF and EAF resulted to be antimutagenic, HF and ALF showed antifungal activity, BF and ACF showed the highest antioxidant potential, and HF and BF were antibacterial against several strains. Anchovy gut, which to the present study had not been reported for any bioactivity, has antimutagenic, antifungal, antioxidant, and antibacterial compounds, which need to be isolated for full characterization and study. Authors wish to acknowledge CONACyT for the financial support granted to Burgos-Hernández, A., (234469) and Rosas‑Burgos, E.C., (234051) to carry out a sabbatical-research staying at the Universidad Miguel Hernández de Elche at Orihuela, Alicante, Spain.
- Published
- 2016
6. The comparison of biological activity of chocolates made by different technological procedures
- Author
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Lucia Godočíková, Eva Ivanišová, Július Árvay, Miroslava Kačániová, and Jana Petrová
- Subjects
0301 basic medicine ,theobromine ,030109 nutrition & dietetics ,Antioxidant ,DPPH ,chocolate ,medicine.medical_treatment ,antioxidant activity ,lcsh:TX341-641 ,Crude fibre ,03 medical and health sciences ,chemistry.chemical_compound ,Human health ,chemistry ,Polyphenol ,flavonoids ,medicine ,Food science ,Caffeine ,lcsh:Nutrition. Foods and food supply ,Theobromine ,polyphenols ,caffeine ,Food Science ,medicine.drug - Abstract
Chocolate is one of the most consumed delicacies in the world. Nowadays, raw chocolates without vanilla or allergens are getting more attention. The aim of this study was to evaluate and compare the biological activity of different types of chocolate - cold processed chocolate and chocolate made by traditional way. Total content of fat, crude fibre, polyphenols, flavonoids, phenolic acids and metylxantines - theobromine and caffeine was evaluated. The antioxidant activity was determined by amethod using DPPH radical, reducing power method and phosphomolybdenum method. Both evaluated chocolates had similar content of fat and crude fibre, but sample of chocolate made by traditional way probably due to the higher content of cocoa mass had almost two times higher content of total polyphenols, flavonoids and phenolic acids as cold processed chocolate. Also the content of theobromine and caffeine was slightly higher in chocolate made by traditional way. This sample had the highest antioxidant activity - 93.68 mg TEAC.g-1 determined by phosphomolybdenum method, while in the sample of chocolate made by cold processed way was measeured value 50.82 mg TEAC.g-1. Similarly, reducing power of chocolate made by traditional way was almost two times higher, but antioxidant activity determined with DPPH method was similar in both samples (3.58 and 3.62 mg TEAC.g-1). The antioxidants and metylxantines in chocolates determine its potential to be asignificant source of biologicaly active compounds withfavorable effects to human health. It can be concluded in this study, that chocolate produced by conventional production technology can have more health-promoting ingredients reserved, but more extensive researches are still needed. 
- Published
- 2016
7. MICROBIOTA OF PINUS POLLEN AS ADJUVANT FACTOR OF ALLERGY
- Author
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Ján Brindza, Tetiana Shevtsova, Kateryna Garkava, Miroslava Kačániová, and Jana Petrová
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Allergy ,010504 meteorology & atmospheric sciences ,medicine.medical_treatment ,lcsh:QR1-502 ,Pinus species ,Biology ,medicine.disease_cause ,01 natural sciences ,Microbiology ,lcsh:Microbiology ,03 medical and health sciences ,0302 clinical medicine ,Pollen contamination ,Pollen ,Botany ,microbiota ,medicine ,microbiological quality ,Molecular Biology ,0105 earth and related environmental sciences ,flowering ,food and beverages ,pollen contamination ,Microbiological quality ,allergy ,medicine.disease ,%22">Pinus ,030228 respiratory system ,Immunology ,Adjuvant ,Food Science ,Biotechnology - Abstract
Bacteria, their endotoxin and mold found on pollen can be a reason of respiratory symptoms in sensitized individuals. This question concerns an anemophilous pollen more acute. In this work quantitative by dilution plating method and qualitative microbial analysis by MALDI-TOF MS Biotyper of pollen and other plants organs of Pinus sylvestris L., P. nigra Arnold, P. mugo Turra, P. armandii Franch., P. wallichiana A.B. Jacks from Nitra, Slovakia are performed which shows quantitative and species differences in mesophilic aerobic (0.00-6.27 log cfu/g) and anaerobic bacteria (0.00-3.70 log cfu/g), enterococci (0.00 log cfu/g), coliform bacteria (0.00-5.29 log cfu/g), lactobacilli (0.00-4.20 log cfu/g), microscopic fungi and yeasts (2.60-5.29 log cfu/g) content. Representatives of Pseudomonas (14), Bacillus (2), Acinetobacter (1), Arthrobacter (1), Pantoea (1), Klebsiella (1), Penicillium (6), Aspergillus (4), Cladosporium (1), Debaryomyces (1) genera were revealed on pine trees. The allergenic potential of the identified association of microorganisms on pollen has been evaluated based on published data. The results may be useful for aerobiologists, allergists and microbiologists, at least at the local level.
- Published
- 2016
8. Microbial Diversity of Betula Trees: Pollen, Catkins, Leaves Relatively of Flowering
- Author
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Kateryna Garkava, Tetiana Shevtsova, Ján Brindza, Miroslava Kačániová, and Jana Petrová
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0301 basic medicine ,medicine.disease_cause ,lcsh:Agriculture ,03 medical and health sciences ,Pollen ,Botany ,microbiota ,medicine ,microbiological quality ,Cultivar ,lcsh:QH301-705.5 ,flowering ,biology ,fungi ,lcsh:S ,food and beverages ,pollen contamination ,biology.organism_classification ,Pantoea agglomerans ,Yeast ,Coliform bacteria ,030104 developmental biology ,lcsh:Biology (General) ,Catkin ,Betula verrucosa Ehrh ,Bee pollen ,General Agricultural and Biological Sciences ,Mesophile - Abstract
Quantitative microbiological analysis by dilution plating method of pollen and additional male and female catkins, leaves of Betula verrucosa Ehrh. and its two cultivars: ‘Purpurea’ and ‘Youngii’ relatively of flowering period of Betula has been realized with the aim to provide new knowledge of the microbiological quality of anemophilous pollen for processing and its further application. Qualitative microbiological analysis with MALDI-TOF MS Biotyper was used in the identification of aerobic, anaerobic mesophilic bacteria and coliforms. Mixed microbiota was determined, consisting of aerobic (4.68–4.89 log cfu/g) and anaerobic (3.30–3.48 log cfu/g) mesophilic bacteria, lactobacilli (0–3.48 log cfu/g), coliform bacteria (0–4.57 log cfu/g), fungi and yeast (3.78–3.95 log cfu/g) on the pollen grains, that indicates acceptable quality in comparison with the microbiological quality parameters for bee pollen. Pantoea agglomerans was found associated with pollen of Betula verrucosa Ehrh. Recommendations on the collection of anemophilous pollen were established.
- Published
- 2016
9. ANTIMICROBIAL ACTIVITY OF CRUDE ETHANOLIC EXTRACTS FROM SOME MEDICINAL MUSHROOMS
- Author
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Lukáš Hleba, Jana Petrová, Miroslava Kačániová, Martin Rajtar, Jana Hutková, Attila Kántor, Matúš Kompas, and Juraj Čuboň
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0301 basic medicine ,Gram-negative bacteria ,Traditional medicine ,Gram-positive bacteria ,030106 microbiology ,Biology ,010402 general chemistry ,biology.organism_classification ,Antimicrobial ,01 natural sciences ,Microbiology ,0104 chemical sciences ,03 medical and health sciences ,chemistry.chemical_compound ,chemistry ,Medicinal fungi ,Molecular Biology ,Food Science ,Biotechnology - Abstract
In this paper the antimicrobial activity of 1 year old crude ethanolic extracts obtained from Cordyceps sinesis, Laricifomes officinalis, Oudemansiella mucida and Coprinus comatus were investigated. The antimicrobial activities of extracts against two Gram-positive bacteria (Bacillus thuringiensis, Staphylococcus aureus) and two Gram-negative bacteria (Klebsiella pneumoniae, Enterobacter aerogenes) were determined by disk diffusion and microbroth dilution method according by EUCAST in 96-well microplates. Microorganisms were obtained from Czech Collection of Microorganisms. Absorbance after and before the experiment were substracted, converted to binary system and obtained values to Probit analysis were used. Not all macromycetes ethanolic extracts showed antimicrobial activity against tested bacteria. Antimicrobial activity determined by MIC methodology showed extracts from Oudemansiella mucida, Cordyceps sinesis, Coprinus comatus in the tested range. Conversely, the best antimicrobial activity tested by disc diffusion methods showed extract from Laricifomes officinalis. Equally, more better studying of antimicrobial activity in these mushrooms will needed.
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- 2016
10. Yeast diversity in new, still fermenting wine 'federweisser'
- Author
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Jana Petrová, Attila Kántor, Miroslava Kačániová, and Jana Hutková
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0301 basic medicine ,Wine ,new wine ,food.ingredient ,biology ,030106 microbiology ,yeasts ,lcsh:TX341-641 ,Saccharomyces cerevisiae ,biology.organism_classification ,Yeast ,Pichia occidentalis ,03 medical and health sciences ,Horticulture ,food ,Hanseniaspora uvarum ,Botany ,Agar ,MALDI-TOF MS ,Fermentation ,Malt extract agar ,lcsh:Nutrition. Foods and food supply ,Metschnikowia pulcherrima ,Food Science - Abstract
The aim of this study was to isolate and identify yeasts in different new wine "federweisser" samples. We collected the samples at the end of the August 2015 and in the middle of the September 2015. Used 15 new wine samples in this study (5 white and 10 red) were from the local Slovak winemakers. Irsai Oliver (3), Moravian Muscat (2), Agria/Turan (1), Dornfelder (3), Blue Frankish (3), Pinot Noir (1) and Saint Laurent (2). Three cultivation media were used for detection of yeasts in "federweisser" samples. Malt extract agar base (MEA), Wort agar (WA) and Wild yeast medium (WYM) were used for the cultivation of yeasts. Cultivation was performed by spread plate method. Ethanol/formic acid extraction procedure was used for preparation of samples. MALDI-TOF Mass Spectrometer (Microflex LT/SH) (Bruker Daltonics, Germany) was used for the identification of yeasts. We identified seven different strains of Saccharomyces cerevisiae (23; 70%), two strains of Kloeckera apiculata [teleomorph Hanseniaspora uvarum] (7; 21%), and one strain of Pichia kluyveri (1; 3%), Pichia occidentalis [anamorph Candida sorbosa] (1; 3%) and Metschnikowia pulcherrima (1; 3%) in 15 new wine "federweisser" samples. Saccharomyces cerevisiae was dominant species in each new wine sample, and formed creamy convex colonies with circular edge. Metschnikowia pulcherrima formed convex to pulvinate, circular white-pink colored colonies, Kloeckera apiculata formed flat, circular smooth colonies with turquoise center with gray edge, Pichia occidentalis formed irregular pulvinate light-cream colored colonies, and Pichia kluyveri formed turquoise, convex, undulate and smooth colonies on Malt extract agar base with bromocresol green.  
- Published
- 2016
11. ANTIMICROBIAL ACTIVITY OF PULCHERRIMIN PIGMENT PRODUCED BY METSCHNIKOWIA PULCHERRIMA AGAINST VARIOUS YEAST SPECIES
- Author
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Lukáš Hleba, Miroslava Kačániová, Attila Kántor, Jana Hutková, and Jana Petrová
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0301 basic medicine ,biology ,030106 microbiology ,biology.organism_classification ,Antimicrobial ,Microbiology ,Yeast ,03 medical and health sciences ,Pigment ,visual_art ,Botany ,visual_art.visual_art_medium ,Molecular Biology ,Metschnikowia pulcherrima ,Food Science ,Biotechnology - Abstract
Metschnikowia pulcherrima is common yeast on grape berries and can grow on special cultivation media with iron (e.g. FeSO4.7H2O) (max. 0.02 g/L) to produce reddish (maroon) pigment pulcherrimin. Many studies confirm that pulcherrimin has antimicrobial activity against postharvest pathogens, microscopic fungi and other yeast species. In this study, two strains of M. pulcherrima, isolated from grapes and identified by MALDI-TOF mass spectrometry, were used : VML and CBS 610NT. Sixteen yeast species: Aureobasidium (1), Candida (5), Hanseniaspora (1), Kregenvanrija (1), Pichia (2), Rhodotorula (2), Saccharomyces (1), Zygosaccharomyces (2) and Wickerhamomyces (1) were selected to test the antimicrobial capacity of these two M. pulcherrima strains. The results showed CBS 610NT had a stronger ability than VML to inhibit the tested yeasts. Among the yeast, Candida spp. and Pichia manshurica were the most sensitive to the pigment pulcherrimin.
- Published
- 2015
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